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Differential Responses of Emiliania huxleyi (Haptophyta) Strains to Copper Excess 赫胥黎Emiliania huxleyi (haptophyia)菌株对铜过量的差异反应
IF 1.3 4区 生物学 Q3 Agricultural and Biological Sciences Pub Date : 2018-11-01 DOI: 10.7872/crya/v39.iss4.2018.481
D. Mella-Flores, J. Machon, L. Contreras-Porcia, Sabina Mesa–Campbell, P. Dassow
Résumé Emiliania huxleyi est un coccolithophore cosmopolite qui présente un haut degré de variabilité génétique et physiologique aussi bien entre souches qu'entre les étapes de son cycle de vie. Ici, nous avons examiné si les réponses au cuivre (Cu) et au stress oxydatif varient selon l'origine environnementale ou l'étape du cycle de vie. Nous avons comparé les réponses à des concentrations toxiques de Cu et à des expositions à court terme au peroxyde d'hydrogène de douze souches (huit diploïdes et deux paires de souches où haploïdes and diploïdes partagent la même base génétique) provenant de différentes origines océanographiques. Les réponses mesurées comprenaient le taux de croissance, les proportions de coccolithes malformés/incomplètes (souches diploïdes), la motilité (souches haploïdes), la fluorescence variable (Fv/Fm, une mesure de la santé de photosystème), analyses de cytométrie en flux des pourcentages de cellules vivantes et l'accumulation des dérivés réactives de l'oxygène (DRO), et les activités des enzymes ascorbate peroxydase (AP) et glutathion peroxydase (GP). Bien qu'une importante variabilité ait été observable entre les réponses au stress de ces souches, aucune différence statistique n'a été détectée lorsque les souches ont été regroupées par origines. Dans les deux paires diploïdes/haploïdes, les diploïdes ont montré une plus grande sensibilité a l'inhibition de la croissance par Cu que leurs haploïdes correspondants, bien que cette différence n'était significatif que dans le cas de la paire diploide–haploide qui est issue d'un isolât de la mer de Tasman (RCC1216 / RCC1217). Les diploïdes accumulent rapidement DRO en réponse a une exposition aigue a Cu, alors que l'accumulation de DRO dans haploïdes était beaucoup plus faible. Notablement, il n'y a eu pas des différences dans les DRO induites par Cu entre les diploïdes sensibles et résistantes, ou entre les haploïdes sensibles et résistantes. Les différences de sensibilité au Cu entre les souches ont été reflétées dans les différences d'activité AP constitutive, mais aucune différence entre les souches dans l'activité GP constitutive ou induite n'a été détectée. Les différences dans l'activité AP constitutive, mais non dans l'activité GP induite par Cu, reflètent la sensibilité au Cu. Dans l'ensemble, nos résultats montrent une variabilité intraspécifique élevée parmi les génotypes et les phases du cycle de vie de E. huxleyi, ce qui pourrait refléter en partie les différences dans la tolérance au stress oxydatif.
Emiliania huxleyi是一种世界性的球藻,在品系和生命周期阶段之间都表现出高度的遗传和生理变异。在这里,我们研究了对铜(Cu)和氧化应激的反应是否随环境来源或生命周期阶段而变化。我们比较了来自不同海洋来源的12个菌株(8个二倍体和2对单倍体和二倍体具有相同遗传基础的菌株)对铜毒性浓度和短期过氧化氢暴露的反应。实测答案包含的生长速度、比例的coccolithes malformés /不完整(二倍体株)、运动(单倍体品系)、荧光测量变量(Fv / Fm),是一家健康photosystème)、流式细胞分析,预测流量的活细胞的百分比和氧反应衍生的积累(DRO ascorbate过氧化物酶的活动)和(AP)及谷胱甘肽过氧化物酶(GP)。虽然在这些菌株的应激反应之间可以观察到显著的变异性,但当菌株按来源分组时,没有发现统计学差异。单倍体/两双二倍体,二倍体显示更大的敏感性,抑制了增长的Cu及其相应的单倍体,虽然这种显著差异仅在双二倍体—haploide出路是谁的塔斯曼海(isolât RCC1216 / RCC1217)。二倍体在急性铜暴露后迅速积累DRO,而单倍体的DRO积累要低得多。值得注意的是,Cu诱导的DRO在敏感和抗性二倍体之间或敏感和抗性单倍体之间没有差异。菌株间铜敏感性的差异反映在构成性AP活性的差异上,但在构成性和诱导性GP活性方面没有检测到菌株间铜敏感性的差异。组成AP活性的差异,而Cu诱导的GP活性的差异,反映了对Cu的敏感性。总的来说,我们的结果表明,赫胥黎E. huxleyi的基因型和生命周期阶段具有很高的种内变异性,这可能部分反映了抗氧化应激能力的差异。
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引用次数: 5
Molecular Systematics of the Siphonous Green Algal Genus Codium in Eastern Victoria, Australia 澳大利亚维多利亚州东部虹吸绿藻属Codium的分子系统学
IF 1.3 4区 生物学 Q3 Agricultural and Biological Sciences Pub Date : 2018-11-01 DOI: 10.7872/crya/v39.iss4.2018.391
M. Croce, P. Díaz-Tapia, Heroen Verbruggen
Abstract The widely distributed genus Codium is a major component of the marine macroalgal flora and has its largest species diversity in warm-temperate regions. Along the South coast of Australia, Codium is a diverse group, but the species diversity in the easternmost region of Victoria is not well-documented. In this study, we characterized the species diversity of Codium from Croajingolong National Park, Gabo Island Lighthouse Reserve and Cape Howe Marine National Park. The specimens were collected from a wide range of microhabitats during an expedition of the Bush Blitz program, and Codium species were delimited through a combination of molecular analyses of the chloroplast markers tufA and rbcL, and morphological observations. Six species-level clusters were found, corresponding to the native C. australicum, C. harveyi, C. lucasii.1 and C. fragile subsp. novae-zelandiae; and the exotic C. fragile subsp. fragile and C. tenue. The specimens of C. australicum showed variability in the architecture of the thallus that was attributed to the different habitats where they were collected. The utricles of C. harveyi were unusual in having a thick apical cell wall that protruded inwards and had internal projections. The species found in eastern Victoria are typical of the Flindersian and Peronian biogeographic provinces, and the low diversity of Codium species in this region is comparable to that of New South Wales.
Codium属分布广泛,是海洋大型藻区系的重要组成部分,物种多样性在暖温带地区最为丰富。沿澳大利亚南海岸,Codium是一个多样化的群体,但在维多利亚最东部地区的物种多样性没有很好的记录。本研究对croajingdragon国家公园、Gabo岛灯塔保护区和Cape Howe海洋国家公园的Codium物种多样性进行了研究。这些标本是在Bush Blitz计划的一次探险中从广泛的微生境中收集的,通过对叶绿体标记tufA和rbcL的分子分析和形态学观察相结合,对Codium物种进行了划分。共发现6个种级聚类,分别为原生C. australum、C. harveyi、C. lucasii。C.易碎亚种;novae-zelandiae;和外来的C.易碎亚种。C. tenue;australum的标本显示出菌体结构的多样性,这归因于它们收集的不同栖息地。哈维尔果的胞室不寻常的是有一个向内突出的厚的顶细胞壁和内部突出。在维多利亚州东部发现的物种是典型的弗林德斯和庇龙纪生物地理省,该地区的Codium物种多样性与新南威尔士州相当。
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引用次数: 0
Spotting the Spots: The Freshwater Brown Alga Heribaudiella fluviatilis (Areschoug) Svedelius within Stream Communities of Southeastern Europe 发现斑点:欧洲东南部溪流群落中的淡水褐藻Heribaudiella fluviatilis (Areschoug) Svedelius
IF 1.3 4区 生物学 Q3 Agricultural and Biological Sciences Pub Date : 2018-11-01 DOI: 10.7872/crya/v39.iss4.2018.449
N. Koletić, A. Alegro, N. Vuković, A. Rimac, V. Šegota
Abstract This paper reports the occurrence of the freshwater brown alga Heribaudiella fluviatilis (Areschoug) Svedelius in four new localities in southeastern Europe, as well as the first records for Croatia. Additionally, we report here a new type of substrate for H. fluviatilis, on stones in karstic rivers characterized by tufa formations. Despite a generally low number of findings, this and previous studies indicate that the species is not as rare as generally perceived; rather, it is overlooked, under-collected and therefore under-reported. The paper provides detailed diagnostic features of the thalli and habitats where the alga was found, with notes on corresponding vegetation, physical conditions and water chemistry. Additionally, along with the new records of the red alga Hildenbrandia rivularis (Liebmann) J. Agardh in Croatia, the associations of Heribaudiella-Hildenbrandia are reported for streams of Black Sea and Adriatic basins.
摘要报道了东南欧4个新发现的淡水褐藻Heribaudiella fluviatilis (Areschoug) Svedelius的出现,以及克罗地亚的首次记录。此外,我们在这里报告了一种新型的河流嗜血杆菌基质,在以凝灰岩地层为特征的喀斯特河流的石头上。尽管发现的数量普遍较少,但这项研究和以前的研究表明,该物种并不像人们通常认为的那样罕见;相反,它被忽视,收集不足,因此报告不足。本文提供了详细的菌体诊断特征和发现藻类的栖息地,并对相应的植被、物理条件和水化学进行了说明。此外,随着克罗地亚红藻Hildenbrandia rivularis (Liebmann) J. Agardh的新记录,在黑海和亚得里亚海盆地的河流中报道了Heribaudiella-Hildenbrandia的关联。
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引用次数: 6
Acknowledgments 致谢
IF 1.3 4区 生物学 Q3 Agricultural and Biological Sciences Pub Date : 2018-11-01 DOI: 10.7872/crya/v39.iss4.2018.533
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引用次数: 0
Table of volume 39 第三十九卷表
IF 1.3 4区 生物学 Q3 Agricultural and Biological Sciences Pub Date : 2018-11-01 DOI: 10.7872/crya/v39.iss4.2018.531
Chaouti Abdellatif, Reani Abdeltif, Machado, Margarida, Sabour Brahim, KUSBERWolf-Henning COCQUYT Christine, Dubrasquet Hélène, Reyes Janette, Sanchez Ramona Pinochet, Valdivia, Nelson, Marie-Laure
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引用次数: 0
Minouche Knoepffler–Péguy (1934–2018), the ‘Dame de Banyuls’ (the Lady of Banyuls)
IF 1.3 4区 生物学 Q3 Agricultural and Biological Sciences Pub Date : 2018-11-01 DOI: 10.7872/crya/v39.iss4.2018.525
C. Boudouresque
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引用次数: 0
New and Rare Psammothidium Species (Bacillariophyta, Achnanthidiaceae) from Northeastern Siberia 标题西伯利亚东北部沙棘属新罕种(硅藻门,沙棘科)
IF 1.3 4区 生物学 Q3 Agricultural and Biological Sciences Pub Date : 2018-11-01 DOI: 10.7872/crya/v39.iss4.2018.465
Marina G. Potapova
Abstract Three rare Psammothidium species were found in recently surveyed lakes of Northeastern Siberia, Russia. One of these species found in lakes of the Kolyma Lowland, is new for science and is formally described here as Psammothidium onufrii sp. nov. It is morphologically similar to the brackish–water species Achnanthes punctulata, but it lacks a central area on rapheless valve and possesses a large central area on the raphe valve. Psammothidium onufrii is characteristic for tundra thermokarst lakes with moderate mineral content. Psammothidium sacculum previously reported from a few subarctic and arctic locations was found in several lakes of the Kolyma Lowland and of the Magadan District. The frustule ultrastructure of this species is documented for the first time here. The third species, known as Achnanthes obliqua (W. Gregory) Hustedt and transferred here to Psammothidium, is a morphologically distinct and relatively rare diatom occasionally reported from northern regions of Eurasia and Western North America. In Northeastern Siberia it was found in sediments of relatively large shallow lakes with low to moderate mineral content. Detailed morphological characterization of the three Psammothidium species will facilitate their detection in environmental surveys and their use in paleoenvironmental reconstructions.
摘要最近在俄罗斯西伯利亚东北部的湖泊中发现了三种罕见的沙棘。其中一种在科莱马低地的湖泊中发现的物种,在科学上是新的,在这里正式描述为Psammothidium onufrii sp. 11 .它在形态上与咸水物种Achnanthes punctulata相似,但它在无缝瓣上缺乏一个中心区域,而在缝瓣上有一个很大的中心区域。沙尘是冻土带热岩溶湖泊的特征,矿物含量适中。在科雷马低地和马加丹地区的几个湖泊中发现了以前报道的几个亚北极和北极地区的沙棘。本文首次记录了该物种的节肢超微结构。第三种硅藻,被称为Achnanthes obliqua (W. Gregory) Hustedt,并在这里转移到Psammothidium,是一种形态独特且相对罕见的硅藻,偶尔报道来自欧亚大陆北部地区和北美西部。在西伯利亚东北部,它被发现在相对较大的浅湖沉积物中,矿物质含量低至中等。对三种沙棘进行详细的形态鉴定,将有助于其在环境调查中的发现和古环境重建中的应用。
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引用次数: 5
New Taxa Proposed in Cryptogamie, Algologie, vol. 39, 2018 Cryptogamie中提出的新分类群,算法,vol. 39, 2018
IF 1.3 4区 生物学 Q3 Agricultural and Biological Sciences Pub Date : 2018-11-01 DOI: 10.7872/crya/v39.iss4.2018.535
C. E. Wetzel, M. J. Wynne
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引用次数: 0
Notes on the Marine Algae of the Bermudas. 16. Two New Epiphytic Species of Champia (Champiaceae, Rhodymeniales), C. hasselbringii and C. insularis 关于百慕大群岛海藻的注释。标题洋贝壳科洋贝壳属二新种:洋贝壳和洋贝壳
IF 1.3 4区 生物学 Q3 Agricultural and Biological Sciences Pub Date : 2018-11-01 DOI: 10.7872/crya/v39.iss4.2018.431
C. Schneider, Maura K. Griffith, C. Lane, G. Saunders
Abstract Using molecular-assisted alpha taxonomy, we have uncovered two new pseudocryptic species of Champia in Bermuda, as well as have demonstrated that the European C. parvula, a name previously applied to historical Bermuda collections, and C. farlowii recently described from southern New England, USA, are not part of the island flora. We present COI-5P and rbcL, as well as morphological, evidence to describe two endemic species for the islands, C. hasselbringii sp. nov. and C. insularis sp. nov. They are similar species with compressed but variable axes and a wealth of overlapping anatomical characteristics. However, the two species are distinguished not only by their genetics, but also by reproductive features that are commonly found in the small prostrate, epiphytic species. It is possible that C. hasselbringii could be the correct name for species reported in warm waters of the western Atlantic as C. compressa or C. vieillardii, species with type localities in South Africa and New Caledonia, respectively.
摘要利用分子辅助α分类学,我们在百慕大发现了两个新的假隐种,并证明了欧洲的C. parvula(以前用于百慕大历史标本的名称)和最近在美国新英格兰南部描述的C. farlowii不是岛屿植物群的一部分。我们提出了COI-5P和rbcL,以及形态学证据来描述岛上的两个特有物种,C. hasselbringii sp. 11和C. insularis sp. 11 .它们是相似的物种,具有压缩但可变的轴和丰富的重叠解剖特征。然而,这两个物种的区别不仅在于它们的遗传,还在于生殖特征,这些特征在小型匍匐附生物种中很常见。C. hasselbringii可能是在西大西洋温暖水域报告的C. compressa或C. vieillardii物种的正确名称,这些物种的模式地点分别在南非和新喀里多尼亚。
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引用次数: 4
Molecular-Assisted Revision of Red Macroalgal Diversity and Distribution along the Western Antarctic Peninsula and South Shetland Islands 南极半岛西部和南设得兰群岛红藻多样性和分布的分子辅助修正
IF 1.3 4区 生物学 Q3 Agricultural and Biological Sciences Pub Date : 2018-11-01 DOI: 10.7872/crya/v39.iss4.2018.409
Hélène Dubrasquet, Janette Reyes, R. P. Sanchez, N. Valdivia, M. Guillemin
Résumé La flore marine de la zone Antarctique est supposée bien connue car relativement peu d' espèces y sont observées par rapport à d'autres régions du monde. En utilisant la taxonomie traditionnelle combinée aux outils moléculaires, nous avons étudié la diversité et biogéographie des communautés de macroalgues rouges le long de la côte Ouest de la Péninsule Antarctique (WAP) et des îles Shetlands du Sud (SShs) en considérant un gradient bathymétrique. Nous avons également comparé nos deux méthodes d'identification en terme de précision et de nombre de taxa identifiés. Nos résultats ont démontré de faibles et homogènes niveaux de diversité à l'échelle de la zone d'étude comme à échelle locale, avec au total environ 50 taxa identifiés. Nous avons également détecté trois complexes d'espèces cryptiques chez les genres Callophyllis, Curdiea et Georgiella, et amélioré l'identification de nos spécimens puisque 98% ont été identifiés jusqu'à l'espèce grâce grace aux outils moléculaires. Nos résultats ont aussi permis de révéler des différences significatives entre les assemblages de macroalgues rouges appartenant à trois sub-régions, correspondant à des latitudes différentes: SShs et partie Nord de la WAP (≈63°S), la partie centrale de la WAP (≈64°S) et la partie Centre-Sud de la WAP (≈67°S). Nos sub-régions ne correspondent pas à la séparation classique entre SShs/WAP proposée dans les études antérieures, et suggèrent d'une part, que le détroit de Bransfield n'est pas une barrière biogéographique forte pour les macroalgues rouge et d'autre part, que la dynamique actuelle et passée de la couverture de glace a pu jouer un rôle déterminant dans la distribution actuelle des assemblages de macroalgues rouges. Finalement, notre travail a permis de construire une banque de données génétiques associée à un herbier de collection de spécimens, et va permettre d'appuyer des travaux futurs pour mieux comprendre la diversité et distribution des communautés d'algues rouges dans la zone d'étude. Abstract The Antarctic marine flora is well known as a flora composed of relatively few species in comparison with other marine realms. Using molecular taxonomy as complementary tool for traditional taxonomy, we studied red macroalgal diversity and biogeography along the Western Antarctic Peninsula (WAP) and South Shetlands Islands (SShs) coasts and across a bathymetric gradient; in addition, we compared both methods of identification in terms of accuracy and number of observed taxa. Our results show low diversity levels at the regional and local scales, with around 50 taxa registered in total. Molecular tools allowed us to detect putative cryptic species within the genera Callophyllis, Curdiea and Georgiella, and to identify 98% of our specimens at species level. Our results also allowed us to identify significant differences between red macroalgal assemblages of three distinct biogeographic sub-regions: SShs and Northern part of the WAP (at ≈63°S), Central part of the
南极地区的海洋植物群被认为是众所周知的,因为与世界其他地区相比,在那里观察到的物种相对较少。利用传统分类学结合分子工具,我们研究了南极半岛西海岸(WAP)和南设得兰群岛(SShs)红大型藻类群落的多样性和生物地理,并考虑了水深梯度。我们还比较了两种鉴定方法的准确性和鉴定率的数量。我们的结果显示,在研究区域和局部范围内,多样性水平较低且均匀,共鉴定出约50个类群。我们还在Callophyllis属、Curdiea属和Georgiella属中检测了3个隐种复合体,并改进了标本的鉴定,利用分子工具鉴定了98%的物种。我们的结果还揭示了WAP的SShs和北部(≈63°S)、WAP的中部(≈64°S)和WAP的中南部(≈67°S)三个亚区域的大型红藻组合之间的显著差异。分离我们sub-régions并不符合经典SShs / WAP之间拟议的早期研究中,一方面表明,海峡Bransfield并不强大的生物地理屏障,对于红藻和另一方面,当前的势头,并从冰盖中起到了决定性的作用,组件的当前红藻的分布。最后,我们的工作建立了一个与标本采集植物标本室相关的遗传数据库,并将支持未来更好地了解研究区域红藻群落的多样性和分布的工作。= =地理= =根据美国人口普查,该地区的总面积为,其中土地和(2.641平方公里)水。利用分子分类学作为传统分类学的补充工具,我们研究了西南极半岛(WAP)和南设得兰群岛(SShs)沿岸和跨水深梯度的红色大型藻类多样性和生物地理学;此外,我们还比较了两种鉴定方法的准确性和观察到的比率数量。我们的结果显示,在区域和地方尺度上的多样性水平很低,总共记录了大约50个类群。= =地理= =根据美国人口普查,这个县的面积为,其中土地面积为,其中土地面积为。我们的研究结果还使我们能够确定三个不同生物地理子区域的大藻红组合之间的显著差异:WAP的SShs和北部部分(at≈63°S)、WAP的中部部分(at≈64°S)和WAP的中南部部分(at≈67°S)。Our sub-regions do not to the”,相当于分离SShs / WAP in前人权研究所与选择》that the Bransfield海峡is not a strong地理隔离for red macroalgae。由于我们的三个次区域对应三个纬度水平,我们认为过去和现在的海冰扰动动态可能影响了观察到的红藻群落的差异。我们认为,在本工作中建立与代金券标本相关的可访问遗传数据集将有利于今后对该区域大型藻类多样性和分布的研究。
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引用次数: 14
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Cryptogamie Algologie
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