首页 > 最新文献

Turkish Journal of Gastroenterology最新文献

英文 中文
Scleromitrion diffusum (Willd.) R. J. Wang Inhibits Gastric Cancer via ERBB2/ERBB3/PI3K/AKT Pathway. Scleromitrion diffusum (Willd.) R. J. Wang通过ERBB2/ERBB3/PI3K/AKT途径抑制胃癌。
IF 1.4 4区 医学 Q4 GASTROENTEROLOGY & HEPATOLOGY Pub Date : 2024-11-01 DOI: 10.5152/tjg.2024.24152
Wei Ye, Qiu Zhao, Peng Li, Tong Zhou

Background/aims: This study aimed to evaluate the anticarcinogenic potential of Scleromitrion diffusum (Willd.) R. J. Wang (SD) extracts in vitro, along with exploring the underlying compatibility mechanisms.

Materials and methods: Scleromitrion diffusum (Willd.) R. J. Wang extract was prepared and gastric cancer (GC) cells were treated to detect the half maximal inhibitory concentration (IC50)/proliferation and migration/invasion by MTS method and transwell assay. The compatibility mechanisms of SD were analyzed by systems pharmacology strategy, combined with cellular experimental validation.

Results: Scleromitrion diffusum (Willd.) R. J. Wang extract showed inhibitory ability on the proliferation of the GC cell lines dose- and time-dependently. A total of 3 active ingredients are involved in anti-gastric cancer effects of SD, based on the top 50 pathways. The "herb-composition-target-pathway" network showed the multi-target and multi-pathway characteristics of SD. There were 52 related targets shared by SD and GC. The cellular experiments supported that SD significantly reduced ERBB2 and ERBB3 expression levels in GC cells. The overexpression of ERBB2 or ERBB3 partially offset the anti-tumor effects of SD.

Conclusion: Scleromitrion diffusum (Willd.) R. J. Wang inhibited gastric cancer growth and metastasis in vitro, which may be related to the inhibition of the ERBB2/ERBB3/PI3K/AKT pathway.

背景/目的:本研究旨在评估Scleromitrion diffusum (Willd.) R. J. Wang(SD)提取物的体外抗癌潜力,并探索其潜在的相容性机制:制备 Scleromitrion diffusum (Willd.) R. J. Wang(SD)提取物并处理胃癌(GC)细胞,采用 MTS 法和经孔法检测半数最大抑制浓度(IC50)/增殖和迁移/侵袭。通过系统药理学策略,结合细胞实验验证,分析了SD的相容性机制:结果:Scleromitrion diffusum(Willd.)根据前50条途径,共有3种有效成分参与了SD的抗胃癌作用。草药成分-靶点-通路 "网络显示了SD的多靶点、多通路特征。SD和GC共有52个相关靶点。细胞实验证明,SD能显著降低GC细胞中ERBB2和ERBB3的表达水平。ERBB2或ERBB3的过表达部分抵消了SD的抗肿瘤作用:结论:Scleromitrion diffusum (Willd.) R. J. Wang能在体外抑制胃癌的生长和转移,这可能与抑制ERBB2/ERBB3/PI3K/AKT通路有关。
{"title":"Scleromitrion diffusum (Willd.) R. J. Wang Inhibits Gastric Cancer via ERBB2/ERBB3/PI3K/AKT Pathway.","authors":"Wei Ye, Qiu Zhao, Peng Li, Tong Zhou","doi":"10.5152/tjg.2024.24152","DOIUrl":"10.5152/tjg.2024.24152","url":null,"abstract":"<p><strong>Background/aims: </strong>This study aimed to evaluate the anticarcinogenic potential of Scleromitrion diffusum (Willd.) R. J. Wang (SD) extracts in vitro, along with exploring the underlying compatibility mechanisms.</p><p><strong>Materials and methods: </strong>Scleromitrion diffusum (Willd.) R. J. Wang extract was prepared and gastric cancer (GC) cells were treated to detect the half maximal inhibitory concentration (IC50)/proliferation and migration/invasion by MTS method and transwell assay. The compatibility mechanisms of SD were analyzed by systems pharmacology strategy, combined with cellular experimental validation.</p><p><strong>Results: </strong>Scleromitrion diffusum (Willd.) R. J. Wang extract showed inhibitory ability on the proliferation of the GC cell lines dose- and time-dependently. A total of 3 active ingredients are involved in anti-gastric cancer effects of SD, based on the top 50 pathways. The \"herb-composition-target-pathway\" network showed the multi-target and multi-pathway characteristics of SD. There were 52 related targets shared by SD and GC. The cellular experiments supported that SD significantly reduced ERBB2 and ERBB3 expression levels in GC cells. The overexpression of ERBB2 or ERBB3 partially offset the anti-tumor effects of SD.</p><p><strong>Conclusion: </strong>Scleromitrion diffusum (Willd.) R. J. Wang inhibited gastric cancer growth and metastasis in vitro, which may be related to the inhibition of the ERBB2/ERBB3/PI3K/AKT pathway.</p>","PeriodicalId":51205,"journal":{"name":"Turkish Journal of Gastroenterology","volume":"35 11","pages":"831-838"},"PeriodicalIF":1.4,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11562600/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142645126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Mushroom Intoxication in Türkiye: A Nationwide Cohort Study Based on Demographic Trends, Seasonal Variations, and the Impact of Climate Change on Incidence 日本蘑菇中毒:一项基于人口趋势、季节变化和气候变化对发病率影响的全国性队列研究
IF 1.4 4区 医学 Q4 GASTROENTEROLOGY & HEPATOLOGY Pub Date : 2024-10-24 DOI: 10.5152/tjg.2024.24368
Dilara Turan Gökçe, Derya Arı, Naim Ata, Hale Gökcan, Ramazan İdilman, Mustafa Mahir Ülgü, Murat Harputluoglu, Mesut Akarsu, Zeki Karasu, Mustafa Okan Ayvalı, Şuayip Birinci, Meral Akdoğan Kayhan

Background/aims: Mushroom intoxication poses a considerable public health risk due to its potential for severe toxicity and fatality. This study aims to investigate demographic trends, diagnostic locations, and mortality rates of patients with mushroom intoxication.

Materials and methods: This retrospective cohort study utilized data from the National Electronic Database of the Turkish Ministry of Health. The study focused on patients without chronic liver disease or prior liver transplantation presenting with mushroom intoxication between 2018 and 2023. Demographic information, diagnostic locations, and mortality rates were analyzed, considering a six-year period to ensure even seasonal distribution.

Results: Among 30459 individuals admitted with mushroom intoxication, 44.75% were male, with a mean age of 45.84 years. The Black Sea, Marmara, and Central Anatolia regions had the highest number of cases, with specific cities like Tokat, Bolu, Yozgat, and Kastamonu having the highest rates per 100,000 population in 2022. Mushroom intoxication predominantly occurred in May, June, October, and November. Hospitalization occurred in 8.9% of cases, with a 6.6% mortality rate within 90 days and 1.3% progressing to liver transplantation. Notably, mushroom intoxication cases increased by 130% in the first half of 2023, particularly in May and June, correlating with increased rainfall.

Conclusion: Mushroom intoxication is a serious public health issue, with morbidity and mortality influenced by climate factors. The study highlights a significant increase in cases in the first half of 2023, potentially linked to heightened rainfall and climate change.

背景/目的:蘑菇中毒因其潜在的严重毒性和致死率而构成相当大的公共卫生风险。本研究旨在调查蘑菇中毒患者的人口趋势、诊断地点和死亡率。材料和方法:这项回顾性队列研究利用了土耳其卫生部国家电子数据库的数据。该研究的重点是2018年至2023年期间没有慢性肝病或既往肝移植的蘑菇中毒患者。分析了人口统计信息、诊断地点和死亡率,考虑到六年的时间,以确保均匀的季节性分布。结果:30459例食用菌中毒患者中,男性占44.75%,平均年龄45.84岁。黑海、马尔马拉和安纳托利亚中部地区的病例数量最多,2022年,托卡特、博卢、约兹加特和卡斯塔莫努等特定城市每10万人的发病率最高。蘑菇中毒主要发生在5月、6月、10月和11月。8.9%的病例住院治疗,90天内死亡率为6.6%,1.3%进展为肝移植。值得注意的是,蘑菇中毒病例在2023年上半年增加了130%,特别是在5月和6月,这与降雨量增加有关。结论:食用菌中毒是严重的公共卫生问题,其发病率和死亡率受气候因素的影响。该研究强调,2023年上半年病例显著增加,可能与降雨量增加和气候变化有关。
{"title":"Mushroom Intoxication in Türkiye: A Nationwide Cohort Study Based on Demographic Trends, Seasonal Variations, and the Impact of Climate Change on Incidence","authors":"Dilara Turan Gökçe, Derya Arı, Naim Ata, Hale Gökcan, Ramazan İdilman, Mustafa Mahir Ülgü, Murat Harputluoglu, Mesut Akarsu, Zeki Karasu, Mustafa Okan Ayvalı, Şuayip Birinci, Meral Akdoğan Kayhan","doi":"10.5152/tjg.2024.24368","DOIUrl":"10.5152/tjg.2024.24368","url":null,"abstract":"<p><strong>Background/aims: </strong>Mushroom intoxication poses a considerable public health risk due to its potential for severe toxicity and fatality. This study aims to investigate demographic trends, diagnostic locations, and mortality rates of patients with mushroom intoxication.</p><p><strong>Materials and methods: </strong>This retrospective cohort study utilized data from the National Electronic Database of the Turkish Ministry of Health. The study focused on patients without chronic liver disease or prior liver transplantation presenting with mushroom intoxication between 2018 and 2023. Demographic information, diagnostic locations, and mortality rates were analyzed, considering a six-year period to ensure even seasonal distribution.</p><p><strong>Results: </strong>Among 30459 individuals admitted with mushroom intoxication, 44.75% were male, with a mean age of 45.84 years. The Black Sea, Marmara, and Central Anatolia regions had the highest number of cases, with specific cities like Tokat, Bolu, Yozgat, and Kastamonu having the highest rates per 100,000 population in 2022. Mushroom intoxication predominantly occurred in May, June, October, and November. Hospitalization occurred in 8.9% of cases, with a 6.6% mortality rate within 90 days and 1.3% progressing to liver transplantation. Notably, mushroom intoxication cases increased by 130% in the first half of 2023, particularly in May and June, correlating with increased rainfall.</p><p><strong>Conclusion: </strong>Mushroom intoxication is a serious public health issue, with morbidity and mortality influenced by climate factors. The study highlights a significant increase in cases in the first half of 2023, potentially linked to heightened rainfall and climate change.</p>","PeriodicalId":51205,"journal":{"name":"Turkish Journal of Gastroenterology","volume":" ","pages":"61-66"},"PeriodicalIF":1.4,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11736803/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142781573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Machine Learning Diagnostic Model for Hepatocellular Carcinoma Based on Liquid-Liquid Phase Separation and Ferroptosis-Related Genes. 基于液-液相分离和凋亡相关基因的肝癌机器学习诊断模型。
IF 1.4 4区 医学 Q4 GASTROENTEROLOGY & HEPATOLOGY Pub Date : 2024-10-07 DOI: 10.5152/tjg.2024.24101
Wenchao Chen, Ting Zhu, Xiaofan Pu, Linlin Zhao, Senhao Zhou, Xin Zhong, Suihan Wang, Tianyu Lin

Background/aims: Hepatocellular carcinoma (HCC) represents a primary liver malignancy with a multifaceted molecular landscape. The interplay between liquid-liquid phase separation (LLPS) and ferroptosis-a regulated form of cell death-has garnered interest in tumorigenesis. However, the precise role of LLPS and ferroptosis-related genes in HCC progression and prognosis remains obscure. Unraveling this connection could pave the way for innovative diagnosis and therapeutic strategies.

Materials and methods: The differentially expressed genes (DEGs) were identified based on 3 GEO datasets, followed by overlapping with LLPS-related and ferroptosis-related genes. Based on central hub genes, a diagnostic model was developed through LASSO regression and validated using KM survival analysis and real-time quantitative polymerase chain reaction (RT-qPCR). Then the effects of NRAS on the development of HCC and ferroptosis were also detected.

Results: We identified 24 DEGs overlapping among HCC-specific, LLPS, and ferroptosis-related genes. A diagnostic model, centered on 5 hub genes, was developed and validated. Lower expression of these genes corresponded with enhanced patient survival rates, and they were distinctly overexpressed in HCC cells. NRAS downregulation significantly inhibited HepG2 cell proliferation and migration (P < .01). Fe2+ content and ROS levels were both significantly increased in the si-NRAS group when compared to those in the si-NC group (P < .01), while opposite results were observed for the protein level of GPX4 and GSH content.

Conclusion: The diagnostic model with 5 hub genes (EZH2, HSPB1, NRAS, RPL8, and SUV39H1) emerges as a potential innovative tool for the diagnosis of HCC. NRAS promotes the carcinogenesis of HCC cells and inhibits ferroptosis.

背景/目的:肝细胞癌(HCC)是一种具有多面分子结构的原发性肝脏恶性肿瘤。液-液相分离(LLPS)和细胞凋亡(一种受调控的细胞死亡形式)之间的相互作用已经引起了人们对肿瘤发生的兴趣。然而,LLPS和凋亡相关基因在HCC进展和预后中的确切作用仍不清楚。解开这种联系可以为创新的诊断和治疗策略铺平道路。材料和方法:基于3个GEO数据集鉴定差异表达基因(DEGs),并与llps相关基因和铁衰相关基因重叠。基于中心枢纽基因,通过LASSO回归建立诊断模型,并使用KM生存分析和实时定量聚合酶链反应(RT-qPCR)进行验证。检测NRAS对肝癌发生及铁下垂的影响。结果:我们在hcc特异性基因、LLPS基因和嗜铁相关基因中发现了24个基因重叠。建立了以5个枢纽基因为中心的诊断模型并进行了验证。这些基因的低表达与患者生存率的提高相对应,并且它们在HCC细胞中明显过表达。NRAS下调可显著抑制HepG2细胞的增殖和迁移(P < 0.01)。与si-NC组相比,si-NRAS组的Fe2+含量和ROS水平均显著升高(P < 0.01), GPX4蛋白水平和GSH含量则相反。结论:包含5个中心基因(EZH2、HSPB1、NRAS、RPL8和SUV39H1)的诊断模型有望成为HCC诊断的创新工具。NRAS促进肝癌细胞的癌变,抑制铁下垂。
{"title":"Machine Learning Diagnostic Model for Hepatocellular Carcinoma Based on Liquid-Liquid Phase Separation and Ferroptosis-Related Genes.","authors":"Wenchao Chen, Ting Zhu, Xiaofan Pu, Linlin Zhao, Senhao Zhou, Xin Zhong, Suihan Wang, Tianyu Lin","doi":"10.5152/tjg.2024.24101","DOIUrl":"10.5152/tjg.2024.24101","url":null,"abstract":"<p><strong>Background/aims: </strong>Hepatocellular carcinoma (HCC) represents a primary liver malignancy with a multifaceted molecular landscape. The interplay between liquid-liquid phase separation (LLPS) and ferroptosis-a regulated form of cell death-has garnered interest in tumorigenesis. However, the precise role of LLPS and ferroptosis-related genes in HCC progression and prognosis remains obscure. Unraveling this connection could pave the way for innovative diagnosis and therapeutic strategies.</p><p><strong>Materials and methods: </strong>The differentially expressed genes (DEGs) were identified based on 3 GEO datasets, followed by overlapping with LLPS-related and ferroptosis-related genes. Based on central hub genes, a diagnostic model was developed through LASSO regression and validated using KM survival analysis and real-time quantitative polymerase chain reaction (RT-qPCR). Then the effects of NRAS on the development of HCC and ferroptosis were also detected.</p><p><strong>Results: </strong>We identified 24 DEGs overlapping among HCC-specific, LLPS, and ferroptosis-related genes. A diagnostic model, centered on 5 hub genes, was developed and validated. Lower expression of these genes corresponded with enhanced patient survival rates, and they were distinctly overexpressed in HCC cells. NRAS downregulation significantly inhibited HepG2 cell proliferation and migration (P < .01). Fe2+ content and ROS levels were both significantly increased in the si-NRAS group when compared to those in the si-NC group (P < .01), while opposite results were observed for the protein level of GPX4 and GSH content.</p><p><strong>Conclusion: </strong>The diagnostic model with 5 hub genes (EZH2, HSPB1, NRAS, RPL8, and SUV39H1) emerges as a potential innovative tool for the diagnosis of HCC. NRAS promotes the carcinogenesis of HCC cells and inhibits ferroptosis.</p>","PeriodicalId":51205,"journal":{"name":"Turkish Journal of Gastroenterology","volume":" ","pages":"89-99"},"PeriodicalIF":1.4,"publicationDate":"2024-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11843271/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142781345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A Rare Cause of Severe Lower Gastrointestinal Bleeding in Ulcerative Colitis Patients in Remission: Giant Pseudopolyps. 溃疡性结肠炎缓解期患者严重下消化道出血的罕见原因:巨大假性息肉。
IF 1.4 4区 医学 Q4 GASTROENTEROLOGY & HEPATOLOGY Pub Date : 2024-10-07 DOI: 10.5152/tjg.2024.24274
Ali Atay, Ilhami Yuksel
{"title":"A Rare Cause of Severe Lower Gastrointestinal Bleeding in Ulcerative Colitis Patients in Remission: Giant Pseudopolyps.","authors":"Ali Atay, Ilhami Yuksel","doi":"10.5152/tjg.2024.24274","DOIUrl":"10.5152/tjg.2024.24274","url":null,"abstract":"","PeriodicalId":51205,"journal":{"name":"Turkish Journal of Gastroenterology","volume":" ","pages":"131-132"},"PeriodicalIF":1.4,"publicationDate":"2024-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11843260/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142780912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Circ_0008035 Promotes Gastric Cancer Development via the miR-429/SMAD2 Cascade. Circ_0008035 通过 miR-429/SMAD2 级联促进胃癌发展
IF 1.4 4区 医学 Q4 GASTROENTEROLOGY & HEPATOLOGY Pub Date : 2024-10-01 DOI: 10.5152/tjg.2024.23341
Yan Chen, Weigang Bian, Surong Chen

The vital roles of circular RNAs (circRNAs) in human tumorigenesis have attracted more attention. Circ_0008035 is one of the most up-regulated circRNAs in gastric cancer (GC). Herein, we explored the associated mechanism of circ_0008035 in GC. EdU incorporation experiments were performed to monitor cell proliferation ability. Cell cycle progression, apoptosis, angiogenesis, migration, and invasion were analyzed using flow cytometry, Tube formation, and Transwell assays respectively. Protein expression was detected by Western blot. Dual-luciferase reporter experiments were applied to demonstrate the relationship between circ_0008035 or SMAD family member 2 (SMAD2) and microRNA-429 (miR-429). Mouse xenograft assays were conducted for evaluation of the role of circ_0008035 in vivo. Circ_0008035 content was elevated in GC tissues (P < .0001) and cell lines (P < .001), and its deficiency hindered GC cell proliferation (P < .01), HUVEC angiogenesis (P < .05), and GC cell metastasis (P < .01) and triggered apoptosis (P < .01). Circ_0008035 could sponge miR-429 to up-regulate SMAD2 expression (P < .0001). Circ_0008035 absence restrained tumor growth in vivo (P < .01). MiR429 was a mediator of circ_0008035 function, and miR-429 hindered GC cell malignant phenotypes by SMAD2. Circ_0008035 aggravates GC cell malignant progression partially by targeting the miR-429/SMAD2 axis. Considering the inhibitory effect of circ_0008035 deficiency on GC progression, targeting circ_0008035 may be a potential approach to prevent or treat GC.

循环 RNA(circRNA)在人类肿瘤发生中的重要作用已引起越来越多的关注。circ_0008035是胃癌(GC)中上调最多的circRNA之一。在此,我们探讨了circ_0008035在胃癌中的相关机制。我们进行了EdU掺入实验来监测细胞的增殖能力。使用流式细胞术、试管形成和 Transwell 试验分别分析了细胞周期进展、细胞凋亡、血管生成、迁移和侵袭。蛋白表达采用 Western 印迹法检测。应用双荧光素酶报告实验来证明 circ_0008035 或 SMAD 家族成员 2(SMAD2)与 microRNA-429 (miR-429)之间的关系。小鼠异种移植实验用于评估 circ_0008035 在体内的作用。Circ_0008035在GC组织(P < .0001)和细胞系(P < .001)中含量升高,其缺乏会阻碍GC细胞增殖(P < .01)、HUVEC血管生成(P < .05)和GC细胞转移(P < .01),并引发细胞凋亡(P < .01)。Circ_0008035 可以海绵状表达 miR-429,从而上调 SMAD2 的表达(P < .0001)。缺少Circ_0008035会抑制体内肿瘤的生长(P < .01)。MiR 429是circ_0008035功能的介导因子,miR-429通过SMAD2阻碍了GC细胞的恶性表型。Circ_0008035通过靶向miR-429/SMAD2轴,部分加剧了GC细胞的恶性进展。考虑到circ_0008035缺乏对GC进展的抑制作用,靶向circ_0008035可能是预防或治疗GC的一种潜在方法。
{"title":"Circ_0008035 Promotes Gastric Cancer Development via the miR-429/SMAD2 Cascade.","authors":"Yan Chen, Weigang Bian, Surong Chen","doi":"10.5152/tjg.2024.23341","DOIUrl":"https://doi.org/10.5152/tjg.2024.23341","url":null,"abstract":"<p><p>The vital roles of circular RNAs (circRNAs) in human tumorigenesis have attracted more attention. Circ_0008035 is one of the most up-regulated circRNAs in gastric cancer (GC). Herein, we explored the associated mechanism of circ_0008035 in GC. EdU incorporation experiments were performed to monitor cell proliferation ability. Cell cycle progression, apoptosis, angiogenesis, migration, and invasion were analyzed using flow cytometry, Tube formation, and Transwell assays respectively. Protein expression was detected by Western blot. Dual-luciferase reporter experiments were applied to demonstrate the relationship between circ_0008035 or SMAD family member 2 (SMAD2) and microRNA-429 (miR-429). Mouse xenograft assays were conducted for evaluation of the role of circ_0008035 in vivo. Circ_0008035 content was elevated in GC tissues (P < .0001) and cell lines (P < .001), and its deficiency hindered GC cell proliferation (P < .01), HUVEC angiogenesis (P < .05), and GC cell metastasis (P < .01) and triggered apoptosis (P < .01). Circ_0008035 could sponge miR-429 to up-regulate SMAD2 expression (P < .0001). Circ_0008035 absence restrained tumor growth in vivo (P < .01). MiR429 was a mediator of circ_0008035 function, and miR-429 hindered GC cell malignant phenotypes by SMAD2. Circ_0008035 aggravates GC cell malignant progression partially by targeting the miR-429/SMAD2 axis. Considering the inhibitory effect of circ_0008035 deficiency on GC progression, targeting circ_0008035 may be a potential approach to prevent or treat GC.</p>","PeriodicalId":51205,"journal":{"name":"Turkish Journal of Gastroenterology","volume":"35 10","pages":"795-804"},"PeriodicalIF":1.4,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11465158/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142480140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Inhibitory Effect of Aconitine on Colorectal Cancer Malignancy via Inducing Apoptosis and Suppression of Cell Motion. 乌头碱通过诱导凋亡和抑制细胞运动对结直肠癌恶性肿瘤的抑制作用。
IF 1.4 4区 医学 Q4 GASTROENTEROLOGY & HEPATOLOGY Pub Date : 2024-09-30 DOI: 10.5152/tjg.2024.24142
Xuehui Li, Jianglin Hu, Duan Tong, Taotao Yang, Ming Deng

Background/aims: The incidence of colorectal cancer (CRC) has been increasing in recent years worldwide. Aconitine is a diester diterpenoid alkaloid that exhibits an antitumor role in several cancers. Nevertheless, it remains unclear whether aconitine also has antitumor activity in CRC. This study aims to investigate the effects of aconitine on the malignant behaviors of CRC cells.

Materials and methods: 3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide (MTT) assay was utilized for cell viability assessment. Flow cytometry, western blotting, wound healing, and Transwell assays were implemented for examining the aconitine effect on CRC cell apoptosis, migration, and invasiveness. Animal experiments were performed to further elucidate aconitine's effect on CRC tumorigenesis.

Results: Aconitine time- and dose-dependently restrained CRC cell viability but was not cytotoxic to normal colorectal mucosa cells. Aconitine facilitated CRC cell apoptosis and hindered cell migration and invasiveness. Aconitine blocked tumor growth in xenograft mouse models.

Conclusion: Aconitine exerts an anti-CRC effect by promoting cell apoptosis and blocking cell migration and invasiveness.

背景/目的:近年来,世界范围内结直肠癌(CRC)的发病率呈上升趋势。乌头碱是一种二酯二萜生物碱,在几种癌症中具有抗肿瘤作用。然而,乌头碱在结直肠癌中是否也具有抗肿瘤活性尚不清楚。本研究旨在探讨乌头碱对结直肠癌细胞恶性行为的影响。材料与方法:采用3-(4,5 -二甲基噻唑-2)- 2,5 -二苯基溴化四唑(MTT)法测定细胞活力。采用流式细胞术、western blotting、伤口愈合和Transwell实验检测乌头碱对结直肠癌细胞凋亡、迁移和侵袭的影响。动物实验进一步阐明乌头碱在结直肠癌发生中的作用。结果:乌头碱具有时间和剂量依赖性,可抑制结直肠癌细胞活力,但对正常结肠粘膜细胞无细胞毒性。乌头碱促进结直肠癌细胞凋亡,阻碍细胞迁移和侵袭。乌头碱阻断异种移植小鼠模型肿瘤生长。结论:乌头碱通过促进细胞凋亡、阻断细胞迁移和侵袭作用,具有抗结直肠癌的作用。
{"title":"Inhibitory Effect of Aconitine on Colorectal Cancer Malignancy via Inducing Apoptosis and Suppression of Cell Motion.","authors":"Xuehui Li, Jianglin Hu, Duan Tong, Taotao Yang, Ming Deng","doi":"10.5152/tjg.2024.24142","DOIUrl":"10.5152/tjg.2024.24142","url":null,"abstract":"<p><strong>Background/aims: </strong>The incidence of colorectal cancer (CRC) has been increasing in recent years worldwide. Aconitine is a diester diterpenoid alkaloid that exhibits an antitumor role in several cancers. Nevertheless, it remains unclear whether aconitine also has antitumor activity in CRC. This study aims to investigate the effects of aconitine on the malignant behaviors of CRC cells.</p><p><strong>Materials and methods: </strong>3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide (MTT) assay was utilized for cell viability assessment. Flow cytometry, western blotting, wound healing, and Transwell assays were implemented for examining the aconitine effect on CRC cell apoptosis, migration, and invasiveness. Animal experiments were performed to further elucidate aconitine's effect on CRC tumorigenesis.</p><p><strong>Results: </strong>Aconitine time- and dose-dependently restrained CRC cell viability but was not cytotoxic to normal colorectal mucosa cells. Aconitine facilitated CRC cell apoptosis and hindered cell migration and invasiveness. Aconitine blocked tumor growth in xenograft mouse models.</p><p><strong>Conclusion: </strong>Aconitine exerts an anti-CRC effect by promoting cell apoptosis and blocking cell migration and invasiveness.</p>","PeriodicalId":51205,"journal":{"name":"Turkish Journal of Gastroenterology","volume":" ","pages":"53-60"},"PeriodicalIF":1.4,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11736829/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142787633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Selenium Attenuates Ethanol-induced Hepatocellular Injury by Regulating Ferroptosis and Apoptosis. 硒通过调节铁凋亡和细胞凋亡减轻乙醇诱导的肝细胞损伤
IF 1.4 4区 医学 Q4 GASTROENTEROLOGY & HEPATOLOGY Pub Date : 2024-09-05 DOI: 10.5152/tjg.2024.24159
Feng Chen, Qianhui Li, Xiaomin Xu, Fei Wang

Ferroptosis is a newly identified type of cell death which is strongly linked to the development of several diseases. Whereas, the role of ferroptosis in the improvement of ethanol-induced hepatocytes injury by selenium has not been confirmed. In this study, an in vitro cell damage model was established using half inhibition concentration of ethanol to induce NCTC clone 1469. Cell activity, lipid peroxidation, apoptosis and the expression of markers related to ferroptosis pathway was determined. A mouse model of alcoholic liver disease (ALD) was constructed and the effectiveness of selenium and ferrostatin-1 in treating ALD in vivo was assessed by serum liver function tests, tissue staining and immunohistochemistry for ferroptosis related proteins. Pretreatment with selenomethionine and ebselen significantly improved ethanol-induced reduction in hepatocyte viability, elevated GSH levels and SOD enzyme activity, reduced MDA and iron content, while improving ethanol-induced changes in apoptosis levels and ferroptosis markers GPX4, SLC7A11, and ACSL4, with the effect of Selenomethionine being more significant. In vivo results also indicated that intervention with selenium or ferroptosis inhibitors significantly improved ethanol-induced liver tissue damage, significantly reduced serum ALT and AST levels, upregulated GPX4 and SLC7A11, but reduced ACSL4 protein levels in liver tissue. The process of ethanol damage to hepatocytes is regulated by the ferroptosis pathway. Selenium may exert a beneficial role in ethanol-induced hepatocyte injury by antagonizing oxidative stress and regulating apoptosis and ferroptosis pathways.

铁突变是一种新发现的细胞死亡类型,与多种疾病的发生密切相关。然而,硒在改善乙醇诱导的肝细胞损伤方面的作用尚未得到证实。本研究使用半抑制浓度的乙醇诱导 NCTC 克隆 1469,建立了体外细胞损伤模型。研究测定了细胞活性、脂质过氧化、细胞凋亡以及与铁氧化途径相关的标志物的表达。构建了酒精性肝病(ALD)小鼠模型,并通过血清肝功能检测、组织染色和铁氧化相关蛋白的免疫组化来评估硒和铁前列素-1治疗体内酒精性肝病的效果。硒蛋氨酸和依布硒的预处理能明显改善乙醇诱导的肝细胞活力下降、GSH水平和SOD酶活性升高、MDA和铁含量降低,同时改善乙醇诱导的肝细胞凋亡水平和铁变态标志物GPX4、SLC7A11和ACSL4的变化,其中硒蛋氨酸的效果更为显著。体内研究结果也表明,硒或铁变态反应抑制剂的干预能明显改善乙醇诱导的肝组织损伤,显著降低血清谷丙转氨酶和谷草转氨酶水平,上调肝组织中 GPX4 和 SLC7A11,但降低 ACSL4 蛋白水平。乙醇对肝细胞的损伤过程受铁蛋白沉积途径的调控。硒可通过拮抗氧化应激、调节细胞凋亡和铁凋亡途径,在乙醇诱导的肝细胞损伤中发挥有益作用。
{"title":"Selenium Attenuates Ethanol-induced Hepatocellular Injury by Regulating Ferroptosis and Apoptosis.","authors":"Feng Chen, Qianhui Li, Xiaomin Xu, Fei Wang","doi":"10.5152/tjg.2024.24159","DOIUrl":"https://doi.org/10.5152/tjg.2024.24159","url":null,"abstract":"<p><p>Ferroptosis is a newly identified type of cell death which is strongly linked to the development of several diseases. Whereas, the role of ferroptosis in the improvement of ethanol-induced hepatocytes injury by selenium has not been confirmed. In this study, an in vitro cell damage model was established using half inhibition concentration of ethanol to induce NCTC clone 1469. Cell activity, lipid peroxidation, apoptosis and the expression of markers related to ferroptosis pathway was determined. A mouse model of alcoholic liver disease (ALD) was constructed and the effectiveness of selenium and ferrostatin-1 in treating ALD in vivo was assessed by serum liver function tests, tissue staining and immunohistochemistry for ferroptosis related proteins. Pretreatment with selenomethionine and ebselen significantly improved ethanol-induced reduction in hepatocyte viability, elevated GSH levels and SOD enzyme activity, reduced MDA and iron content, while improving ethanol-induced changes in apoptosis levels and ferroptosis markers GPX4, SLC7A11, and ACSL4, with the effect of Selenomethionine being more significant. In vivo results also indicated that intervention with selenium or ferroptosis inhibitors significantly improved ethanol-induced liver tissue damage, significantly reduced serum ALT and AST levels, upregulated GPX4 and SLC7A11, but reduced ACSL4 protein levels in liver tissue. The process of ethanol damage to hepatocytes is regulated by the ferroptosis pathway. Selenium may exert a beneficial role in ethanol-induced hepatocyte injury by antagonizing oxidative stress and regulating apoptosis and ferroptosis pathways.</p>","PeriodicalId":51205,"journal":{"name":"Turkish Journal of Gastroenterology","volume":"35 10","pages":"778-786"},"PeriodicalIF":1.4,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11465188/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142480142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The Impact and Mechanism of Action of Peptostreptococcus anaerobius on Chemotherapy Resistance in Human Colorectal Cancer. 厌氧肽链球菌对人类结直肠癌耐药性的影响和作用机制
IF 1.4 4区 医学 Q4 GASTROENTEROLOGY & HEPATOLOGY Pub Date : 2024-09-05 DOI: 10.5152/tjg.2024.24221
Guangcai Li, Wenwen Li, Hongsheng Dai, Xialian He, Lihong Shi, Xiaoqian Zhang

Peptostreptococcus anaerobius plays an important role in the development of colorectal cancer, and previous studies by our group have demonstrated that Peptostreptococcus anaerobius promotes resistance to 5-Fu chemotherapy in animal models of colorectal cancer. In this study, the effects of Peptostreptococcus anaerobius on chemotherapy resistance in colorectal cancer and its possible mechanism of action were investigated from the clinical point of view. Patients were selected according to exclusion and inclusion criteria and divided into sensitive and chemotherapy groups (n = 20/group). Fecal samples were collected from the patients. The bacterial 16S rRNA genes in the samples were sequenced and the abundance and varieties in the fecal bacteria were compared between the 2 groups. Immunohistochemistry and Western blotting were used to assess interleukin 23 levels in tumor tissues. Significantly elevated abundance of Peptostreptococcus was observed in fecal samples from chemoresistant colorectal cancer patients compared to those from chemosensitive individuals. Immunohistochemistry and Western blotting results showed that chemoresistant patients had higher levels of interleukin 23 relative to chemosensitive patients and the levels were positively associated with Peptostreptococcus. Peptostreptococcus may mediate the development of chemoresistant colorectal cancer by promoting the upregulation of interleukin 23. Efforts to target Peptostreptococcus thus have the potential to alter the prognosis of colorectal cancer patients.

厌氧链球菌在结直肠癌的发病过程中起着重要作用,我们小组之前的研究表明,厌氧链球菌能促进结直肠癌动物模型对 5-Fu 化疗的耐药性。本研究从临床角度探讨了厌氧链球菌对结直肠癌化疗耐药的影响及其可能的作用机制。根据排除和纳入标准选择患者,将其分为敏感组和化疗组(n = 20/组)。研究人员采集了患者的粪便样本。对样本中的细菌 16S rRNA 基因进行测序,并比较两组患者粪便中细菌的数量和种类。免疫组化和 Western 印迹技术用于评估肿瘤组织中白细胞介素 23 的水平。与化疗敏感者的粪便样本相比,化疗耐受性结直肠癌患者粪便样本中肽链球菌的数量明显增加。免疫组织化学和 Western 印迹分析结果显示,化疗耐受性患者的白细胞介素 23 水平高于化疗敏感性患者,且白细胞介素 23 水平与eptostreptococcus 呈正相关。肽链球菌可能通过促进白细胞介素 23 的上调,介导化疗耐药结直肠癌的发展。因此,针对肽链球菌所做的努力有可能改变结直肠癌患者的预后。
{"title":"The Impact and Mechanism of Action of Peptostreptococcus anaerobius on Chemotherapy Resistance in Human Colorectal Cancer.","authors":"Guangcai Li, Wenwen Li, Hongsheng Dai, Xialian He, Lihong Shi, Xiaoqian Zhang","doi":"10.5152/tjg.2024.24221","DOIUrl":"https://doi.org/10.5152/tjg.2024.24221","url":null,"abstract":"<p><p>Peptostreptococcus anaerobius plays an important role in the development of colorectal cancer, and previous studies by our group have demonstrated that Peptostreptococcus anaerobius promotes resistance to 5-Fu chemotherapy in animal models of colorectal cancer. In this study, the effects of Peptostreptococcus anaerobius on chemotherapy resistance in colorectal cancer and its possible mechanism of action were investigated from the clinical point of view. Patients were selected according to exclusion and inclusion criteria and divided into sensitive and chemotherapy groups (n = 20/group). Fecal samples were collected from the patients. The bacterial 16S rRNA genes in the samples were sequenced and the abundance and varieties in the fecal bacteria were compared between the 2 groups. Immunohistochemistry and Western blotting were used to assess interleukin 23 levels in tumor tissues. Significantly elevated abundance of Peptostreptococcus was observed in fecal samples from chemoresistant colorectal cancer patients compared to those from chemosensitive individuals. Immunohistochemistry and Western blotting results showed that chemoresistant patients had higher levels of interleukin 23 relative to chemosensitive patients and the levels were positively associated with Peptostreptococcus. Peptostreptococcus may mediate the development of chemoresistant colorectal cancer by promoting the upregulation of interleukin 23. Efforts to target Peptostreptococcus thus have the potential to alter the prognosis of colorectal cancer patients.</p>","PeriodicalId":51205,"journal":{"name":"Turkish Journal of Gastroenterology","volume":"35 10","pages":"763-771"},"PeriodicalIF":1.4,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11465157/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142480144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Beyond the Usual Suspects: Unraveling the Etiology of Abdominal Pain with Behcet's Disease Intestinal Manifestation. 超越常规疑点:揭开白塞氏病肠道表现腹痛的病因。
IF 1.4 4区 医学 Q4 GASTROENTEROLOGY & HEPATOLOGY Pub Date : 2024-09-04 DOI: 10.5152/tjg.2024.24272
Idrıs Kurt, Abdulvahap Kahveci
{"title":"Beyond the Usual Suspects: Unraveling the Etiology of Abdominal Pain with Behcet's Disease Intestinal Manifestation.","authors":"Idrıs Kurt, Abdulvahap Kahveci","doi":"10.5152/tjg.2024.24272","DOIUrl":"https://doi.org/10.5152/tjg.2024.24272","url":null,"abstract":"","PeriodicalId":51205,"journal":{"name":"Turkish Journal of Gastroenterology","volume":"35 10","pages":"808-810"},"PeriodicalIF":1.4,"publicationDate":"2024-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11465361/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142480138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Esophageal Squamous Papillomatosis Associated Low-Grade Dysplasia Treated with Endoscopic Submucosal Dissection. 食管鳞状乳头状瘤病相关低度发育不良经内镜黏膜下剥离术治疗。
IF 1.4 4区 医学 Q4 GASTROENTEROLOGY & HEPATOLOGY Pub Date : 2024-09-02 DOI: 10.5152/tjg.2024.24178
Abdullah Murat Buyruk, Çağdaş Erdoğan
{"title":"Esophageal Squamous Papillomatosis Associated Low-Grade Dysplasia Treated with Endoscopic Submucosal Dissection.","authors":"Abdullah Murat Buyruk, Çağdaş Erdoğan","doi":"10.5152/tjg.2024.24178","DOIUrl":"10.5152/tjg.2024.24178","url":null,"abstract":"","PeriodicalId":51205,"journal":{"name":"Turkish Journal of Gastroenterology","volume":"35 9","pages":"752-754"},"PeriodicalIF":1.4,"publicationDate":"2024-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11391225/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142331959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
Turkish Journal of Gastroenterology
全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1