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Termite Microbial Symbiosis as a Model for Innovative Design of Lignocellulosic Future Biorefinery: Current Paradigms and Future Perspectives 白蚁微生物共生作为木质纤维素未来生物精炼创新设计的模型:当前范例与未来展望
Pub Date : 2024-03-01 DOI: 10.3390/biomass4010009
M. Dar, Rongrong Xie, H. Zabed, Shehbaz Ali, Daochen Zhu, Jianzhong Sun
The hunt for renewable and alternative fuels has driven research towards the biological conversion of lignocellulosic biomass (LCB) into biofuels, including bioethanol and biohydrogen. Among the natural biomass utilization systems (NBUS), termites represent a unique and easy-to-access model system to study host–microbe interactions towards lignocellulose bioconversion/valorization. Termites have gained significant interest due to their highly efficient lignocellulolytic systems. The wood-feeding termites apply a unique and stepwise process for the hydrolysis of lignin, hemicellulose, and cellulose via biocatalytic processes; therefore, mimicking their digestive metabolism and physiochemical gut environments might lay the foundation for an innovative design of nature-inspired biotechnology. This review highlights the gut system of termites, particularly the wood-feeding species, as a unique model for future biorefinery. The gut system of termites is a treasure-trove for prospecting novel microbial species, including protists, bacteria, and fungi, having higher biocatalytic efficiencies and biotechnological potentials. The significance of potential bacteria and fungi for harnessing the enzymes appropriate for lignocellulosic biorefinery is also discussed. Termite digestomes are rich sources of lignocellulases and related enzymes that could be utilized in various industrial processes and biomass-related applications. Consideration of the host and symbiont as a single functioning unit will be one of the most crucial strategies to expedite developments in termite-modeled biotechnology in the future.
对可再生和替代燃料的追求推动了将木质纤维素生物质(LCB)生物转化为生物燃料(包括生物乙醇和生物氢)的研究。在天然生物质利用系统(NBUS)中,白蚁是研究木质纤维素生物转化/价值化过程中宿主与微生物相互作用的一个独特且易于获取的模型系统。白蚁因其高效的木质纤维素分解系统而备受关注。以木材为食的白蚁通过生物催化过程,采用独特的逐步水解木质素、半纤维素和纤维素的过程;因此,模仿白蚁的消化代谢和肠道理化环境,可能会为创新设计自然启发生物技术奠定基础。本综述强调白蚁的肠道系统,尤其是以木材为食的白蚁,是未来生物炼制的独特模型。白蚁的肠道系统是发掘新型微生物物种(包括原生生物、细菌和真菌)的宝库,这些微生物物种具有更高的生物催化效率和生物技术潜力。此外,还讨论了潜在细菌和真菌对利用适合木质纤维素生物炼制的酶的重要意义。白蚁消化体是木质纤维素酶和相关酶的丰富来源,可用于各种工业流程和生物质相关应用。将宿主和共生体视为一个单一的功能单元,将是未来加快白蚁模式生物技术发展的最重要战略之一。
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引用次数: 0
Orange Peel Waste as a Source of Bioactive Compounds and Valuable Products: Insights Based on Chemical Composition and Biorefining 作为生物活性化合物和有价值产品来源的橘子皮废弃物:基于化学成分和生物精炼的见解
Pub Date : 2024-02-02 DOI: 10.3390/biomass4010006
M. Ortiz-Sanchez, C. A. Cardona Alzate, J. Solarte-Toro
Few valorization pathways have been implemented as alternatives to reduce the orange peel waste (OPW) disposal in landfills. OPW can be a source of income or economic savings in juice production factories since this waste is a potential source of value-added products (e.g., bioactive compounds) and energy vectors (e.g., biogas). Valorization alternatives should be based on (i) orange peel chemical composition, (ii) market analysis, and (iii) availability. Nevertheless, few literature papers have highlighted the chemical composition change caused by the different juice production schemes as a potential opportunity to obtain different value-added products and biorefinery schemes. Thus, the aims of this review paper are related to (i) reviewing different orange fruit processing pathways, (ii) analyzing several OPW chemical compositions reported in the open literature, (iii) providing a summary of OPW extraction pathways for bioactive compounds production, and (iv) evaluating the effect of applying different extraction methods on bioactive compound extraction performance. This review includes a description of the OPW matrix, market insights, packaging, physicochemical characterization, processing technologies, and suggested biorefinery approaches. Finally, different extraction methods for obtaining bioactive compounds from OPW are compared. As a result, the supercritical fluid extraction process has the highest extraction performance and selectivity since this method extracted a high amount of hesperidin (8.18 g/kg OPW db.). In conclusion, OPW is a source of bioactive compounds and valuable products that can be introduced in juice-producing factories to increase product portfolio or economic savings by changing the energy matrix.
作为减少垃圾填埋场橘皮废物(OPW)处理量的替代方法,很少有价值化途径得到实施。橘皮废物可以成为果汁生产厂的收入来源或经济节约,因为这种废物是增值产品(如生物活性化合物)和能源载体(如沼气)的潜在来源。增值替代品应基于 (i) 橙皮化学成分、(ii) 市场分析和 (iii) 可用性。然而,很少有文献强调不同果汁生产方案引起的化学成分变化是获得不同增值产品和生物精炼方案的潜在机会。因此,本综述论文的目的是:(i) 综述不同的橙果加工途径;(ii) 分析公开文献中报道的几种 OPW 化学成分;(iii) 总结生产生物活性化合物的 OPW 提取途径;(iv) 评估应用不同提取方法对生物活性化合物提取性能的影响。本综述包括对 OPW 基质、市场见解、包装、物理化学特征、加工技术和建议的生物精炼方法的描述。最后,比较了从 OPW 中提取生物活性化合物的不同提取方法。结果表明,超临界流体萃取工艺的萃取性能和选择性最高,因为这种方法萃取出了大量橙皮甙(8.18 克/千克 OPW db.)。总之,OPW 是一种生物活性化合物和有价值产品的来源,可以引入果汁生产厂,通过改变能源结构来增加产品组合或节约经济成本。
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引用次数: 0
State of the Art Review of Attributes and Mechanical Properties of Hempcrete 大麻混凝土属性和机械性能的最新进展综述
Pub Date : 2024-02-02 DOI: 10.3390/biomass4010004
Nima Asghari, A. Memari
The global surge in environmental pollution, largely attributed to industrialization, has fueled a pressing need for sustainable solutions. In response, the construction sector is increasingly focusing on bio-based materials such as hemp, recognized for its low environmental footprint and prominent carbon-negative quality. As designers, housebuilders, and an environmentally conscious society pivot towards ecological alternatives to standard building materials, hempcrete emerges as a promising candidate. As a composite material mainly made from hemp hurd/shiv, water, and lime, hempcrete offers the ability to sequester carbon long after its incorporation into structures. As a result, the hemp cultivation process—which can be completed within less than four months—ensures that more carbon is absorbed during production and deployment than emitted, e.g., per one study, sequestration on the order of 300 kg of CO2 per m3 of hempcrete. In comparison to concrete, hempcrete offers a more sustainable footprint, given its recyclability post life cycle. This state-of-the-art review paper delves deep into different aspects of hempcrete, summarizing its multifaceted attributes, particularly its compressive strength. Based on the study conducted, the paper also suggests strategies to augment this strength, thereby transitioning hempcrete from a non-load-bearing material to one capable of shouldering significant weight. As architects and designers consistently strive to align their projects with high ecological standards, focusing not just on aesthetic appeal but also environmental compatibility, hempcrete becomes an increasingly fitting solution for the future of construction.
全球环境污染激增,这主要归咎于工业化,因此迫切需要可持续的解决方案。为此,建筑行业越来越关注生物基材料,如大麻,因为大麻被公认为对环境影响小,具有显著的负碳品质。随着设计师、房屋建筑商和具有环保意识的社会将目光转向标准建筑材料的生态替代品,大麻混凝土应运而生。大麻混凝土是一种主要由大麻秆/秸秆、水和石灰制成的复合材料,它能够在进入建筑物后长期固碳。因此,大麻的种植过程可在不到四个月的时间内完成,确保在生产和使用过程中吸收的碳多于排放的碳,例如,根据一项研究,每立方米大麻混凝土可固存 300 千克二氧化碳。与混凝土相比,由于麻混凝土在生命周期后可回收利用,因此其足迹更具可持续性。这篇最新的综述论文深入探讨了麻混凝土的各个方面,总结了其多方面的属性,尤其是抗压强度。在研究的基础上,论文还提出了增强这种强度的策略,从而将麻混凝土从一种非承重材料转变为一种能够承受较大重量的材料。随着建筑师和设计师不断努力使他们的项目符合高生态标准,不仅注重美学吸引力,而且注重环境兼容性,麻混凝土越来越成为未来建筑的合适解决方案。
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引用次数: 0
State of the Art Review of Attributes and Mechanical Properties of Hempcrete 大麻混凝土属性和机械性能的最新进展综述
Pub Date : 2024-02-02 DOI: 10.3390/biomass4010004
Nima Asghari, A. Memari
The global surge in environmental pollution, largely attributed to industrialization, has fueled a pressing need for sustainable solutions. In response, the construction sector is increasingly focusing on bio-based materials such as hemp, recognized for its low environmental footprint and prominent carbon-negative quality. As designers, housebuilders, and an environmentally conscious society pivot towards ecological alternatives to standard building materials, hempcrete emerges as a promising candidate. As a composite material mainly made from hemp hurd/shiv, water, and lime, hempcrete offers the ability to sequester carbon long after its incorporation into structures. As a result, the hemp cultivation process—which can be completed within less than four months—ensures that more carbon is absorbed during production and deployment than emitted, e.g., per one study, sequestration on the order of 300 kg of CO2 per m3 of hempcrete. In comparison to concrete, hempcrete offers a more sustainable footprint, given its recyclability post life cycle. This state-of-the-art review paper delves deep into different aspects of hempcrete, summarizing its multifaceted attributes, particularly its compressive strength. Based on the study conducted, the paper also suggests strategies to augment this strength, thereby transitioning hempcrete from a non-load-bearing material to one capable of shouldering significant weight. As architects and designers consistently strive to align their projects with high ecological standards, focusing not just on aesthetic appeal but also environmental compatibility, hempcrete becomes an increasingly fitting solution for the future of construction.
全球环境污染激增,这主要归咎于工业化,因此迫切需要可持续的解决方案。为此,建筑行业越来越关注生物基材料,如大麻,因为大麻被公认为对环境影响小,具有显著的负碳品质。随着设计师、房屋建筑商和具有环保意识的社会将目光转向标准建筑材料的生态替代品,大麻混凝土应运而生。大麻混凝土是一种主要由大麻秆/秸秆、水和石灰制成的复合材料,它能够在进入建筑物后长期固碳。因此,大麻的种植过程可在不到四个月的时间内完成,确保在生产和使用过程中吸收的碳多于排放的碳,例如,根据一项研究,每立方米大麻混凝土可固存 300 千克二氧化碳。与混凝土相比,由于麻混凝土在生命周期后可回收利用,因此其足迹更具可持续性。这篇最新的综述论文深入探讨了麻混凝土的各个方面,总结了其多方面的属性,尤其是抗压强度。在研究的基础上,论文还提出了增强这种强度的策略,从而将麻混凝土从一种非承重材料转变为一种能够承受较大重量的材料。随着建筑师和设计师不断努力使他们的项目符合高生态标准,不仅注重美学吸引力,而且注重环境兼容性,麻混凝土越来越成为未来建筑的合适解决方案。
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引用次数: 0
Orange Peel Waste as a Source of Bioactive Compounds and Valuable Products: Insights Based on Chemical Composition and Biorefining 作为生物活性化合物和有价值产品来源的橘子皮废弃物:基于化学成分和生物精炼的见解
Pub Date : 2024-02-02 DOI: 10.3390/biomass4010006
M. Ortiz-Sanchez, C. A. Cardona Alzate, J. Solarte-Toro
Few valorization pathways have been implemented as alternatives to reduce the orange peel waste (OPW) disposal in landfills. OPW can be a source of income or economic savings in juice production factories since this waste is a potential source of value-added products (e.g., bioactive compounds) and energy vectors (e.g., biogas). Valorization alternatives should be based on (i) orange peel chemical composition, (ii) market analysis, and (iii) availability. Nevertheless, few literature papers have highlighted the chemical composition change caused by the different juice production schemes as a potential opportunity to obtain different value-added products and biorefinery schemes. Thus, the aims of this review paper are related to (i) reviewing different orange fruit processing pathways, (ii) analyzing several OPW chemical compositions reported in the open literature, (iii) providing a summary of OPW extraction pathways for bioactive compounds production, and (iv) evaluating the effect of applying different extraction methods on bioactive compound extraction performance. This review includes a description of the OPW matrix, market insights, packaging, physicochemical characterization, processing technologies, and suggested biorefinery approaches. Finally, different extraction methods for obtaining bioactive compounds from OPW are compared. As a result, the supercritical fluid extraction process has the highest extraction performance and selectivity since this method extracted a high amount of hesperidin (8.18 g/kg OPW db.). In conclusion, OPW is a source of bioactive compounds and valuable products that can be introduced in juice-producing factories to increase product portfolio or economic savings by changing the energy matrix.
作为减少垃圾填埋场橘皮废物(OPW)处理量的替代方法,很少有价值化途径得到实施。橘皮废物可以成为果汁生产厂的收入来源或经济节约,因为这种废物是增值产品(如生物活性化合物)和能源载体(如沼气)的潜在来源。增值替代品应基于 (i) 橙皮化学成分、(ii) 市场分析和 (iii) 可用性。然而,很少有文献强调不同果汁生产方案引起的化学成分变化是获得不同增值产品和生物精炼方案的潜在机会。因此,本综述论文的目的是:(i) 综述不同的橙果加工途径;(ii) 分析公开文献中报道的几种 OPW 化学成分;(iii) 总结生产生物活性化合物的 OPW 提取途径;(iv) 评估应用不同提取方法对生物活性化合物提取性能的影响。本综述包括对 OPW 基质、市场见解、包装、物理化学特征、加工技术和建议的生物精炼方法的描述。最后,比较了从 OPW 中提取生物活性化合物的不同提取方法。结果表明,超临界流体萃取工艺的萃取性能和选择性最高,因为这种方法萃取出了大量橙皮甙(8.18 克/千克 OPW db.)。总之,OPW 是一种生物活性化合物和有价值产品的来源,可以引入果汁生产厂,通过改变能源结构来增加产品组合或节约经济成本。
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引用次数: 0
Extraction of Cellulases Produced through Solid-State Fermentation by Trichoderma reesei CCT-2768 Using Green Coconut Fibers Pretreated by Steam Explosion Combined with Alkali 利用经蒸汽爆破结合碱处理的绿椰子纤维提取毛霉 CCT-2768 固态发酵产生的纤维素酶
Pub Date : 2024-02-02 DOI: 10.3390/biomass4010005
A. O. Campos, Estéfani A. Asevedo, Pedro F. Souza Filho, E. S. D. Santos
The industrial processing of coconut to produce valuable foods, such as water and milk, generates large volumes of waste, especially the fruit shell. Despite this, material can be used in bioprocess applications, e.g., the production of enzymes, its recalcitrance hinders the cultivation of microorganisms, and low productivity is usually achieved. In this study, the production of cellulolytic enzymes through solid-state fermentation (SSF) and their extraction was investigated using the green coconut fiber pretreated by steam explosion, followed by alkali. The fungus Trichoderma reesei CCT-2768 was cultivated, using an experimental design, to study the effect of the water activity and the amount of biomass in the reactor. The combination of the pretreatment strategies yielded more porous biomass, with less hemicellulose (5.38%, compared to 10.15% of the raw biomass) and more cellulose (47.77% and 33.96% in the pretreated and raw biomasses, respectively). The water activity significantly affected the production of cellulases, with maximum activity yielded at the highest investigated value (0.995). Lastly, the extraction of the enzymes from the cultivation medium was studied, and a 9 g/L NaCl solution recovered the highest CMCase and FPase activities (5.19 and 1.19 U/g, respectively). This study provides an important contribution to the valorization of the coconut residue through (i) the application of the steam explosion technology to optimize the production of cellulases using the SSF technology and (ii) their extraction using different solvents.
在对椰子进行工业加工以生产水和奶等有价值食品的过程中,会产生大量废物,尤其是果壳。尽管如此,这种材料仍可用于生物工艺应用,如生产酶,但其顽固性阻碍了微生物的培养,通常生产率较低。在本研究中,研究人员利用经蒸汽爆炸预处理后再用碱处理的绿色椰子纤维,通过固态发酵(SSF)生产纤维素分解酶并提取酶。通过实验设计,培养了毛霉 CCT-2768 来研究水活性和反应器中生物量的影响。预处理策略的组合产生了更多的多孔生物质,其中半纤维素较少(5.38%,而原生物质为 10.15%),纤维素较多(在预处理生物质和原生物质中分别为 47.77% 和 33.96%)。水活性对纤维素酶的产生有很大影响,在调查的最高值(0.995)下产生的活性最大。最后,研究了从培养基中提取酶的方法,9 g/L NaCl 溶液回收的 CMCase 和 FPase 活性最高(分别为 5.19 和 1.19 U/g)。这项研究通过(i)应用蒸汽爆炸技术,利用 SSF 技术优化纤维素酶的生产,以及(ii)使用不同溶剂萃取纤维素酶,为椰子残渣的价值化做出了重要贡献。
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引用次数: 0
Extraction of Cellulases Produced through Solid-State Fermentation by Trichoderma reesei CCT-2768 Using Green Coconut Fibers Pretreated by Steam Explosion Combined with Alkali 利用经蒸汽爆破结合碱处理的绿椰子纤维提取毛霉 CCT-2768 固态发酵产生的纤维素酶
Pub Date : 2024-02-02 DOI: 10.3390/biomass4010005
A. O. Campos, Estéfani A. Asevedo, Pedro F. Souza Filho, E. S. D. Santos
The industrial processing of coconut to produce valuable foods, such as water and milk, generates large volumes of waste, especially the fruit shell. Despite this, material can be used in bioprocess applications, e.g., the production of enzymes, its recalcitrance hinders the cultivation of microorganisms, and low productivity is usually achieved. In this study, the production of cellulolytic enzymes through solid-state fermentation (SSF) and their extraction was investigated using the green coconut fiber pretreated by steam explosion, followed by alkali. The fungus Trichoderma reesei CCT-2768 was cultivated, using an experimental design, to study the effect of the water activity and the amount of biomass in the reactor. The combination of the pretreatment strategies yielded more porous biomass, with less hemicellulose (5.38%, compared to 10.15% of the raw biomass) and more cellulose (47.77% and 33.96% in the pretreated and raw biomasses, respectively). The water activity significantly affected the production of cellulases, with maximum activity yielded at the highest investigated value (0.995). Lastly, the extraction of the enzymes from the cultivation medium was studied, and a 9 g/L NaCl solution recovered the highest CMCase and FPase activities (5.19 and 1.19 U/g, respectively). This study provides an important contribution to the valorization of the coconut residue through (i) the application of the steam explosion technology to optimize the production of cellulases using the SSF technology and (ii) their extraction using different solvents.
在对椰子进行工业加工以生产水和奶等有价值食品的过程中,会产生大量废物,尤其是果壳。尽管如此,这种材料仍可用于生物工艺应用,如生产酶,但其顽固性阻碍了微生物的培养,通常生产率较低。在本研究中,研究人员利用经蒸汽爆炸预处理后再用碱处理的绿色椰子纤维,通过固态发酵(SSF)生产纤维素分解酶并提取酶。通过实验设计,培养了毛霉 CCT-2768 来研究水活性和反应器中生物量的影响。预处理策略的组合产生了更多的多孔生物质,其中半纤维素较少(5.38%,而原生物质为 10.15%),纤维素较多(在预处理生物质和原生物质中分别为 47.77% 和 33.96%)。水活性对纤维素酶的产生有很大影响,在调查的最高值(0.995)下产生的活性最大。最后,研究了从培养基中提取酶的方法,9 g/L NaCl 溶液回收的 CMCase 和 FPase 活性最高(分别为 5.19 和 1.19 U/g)。这项研究通过(i)应用蒸汽爆炸技术,利用 SSF 技术优化纤维素酶的生产,以及(ii)使用不同溶剂萃取纤维素酶,为椰子残渣的价值化做出了重要贡献。
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引用次数: 0
Comparative Feasibility and Environmental Life Cycle Assessment of Cotton Stalks Gasification and Pyrolysis 棉秆气化和热解的可行性比较与环境生命周期评估
Pub Date : 2024-01-02 DOI: 10.3390/biomass4010002
I. Vaskalis, A. Zabaniotou
In a circular economy, significant emphasis is given to the energetic valorization of agricultural byproducts. Cotton stalks are suitable as a feedstock for the production of bioenergy due to their high energy content. This study’s main focal areas are the economic viability and environmental implications of a system that can gasify or pyrolyze 25,500 tons of cotton stalk annually. To learn more about how gasification and pyrolysis affect the environment, a life cycle assessment (LCA) was conducted. This analysis evaluates the whole value chain and covers all stages of the cotton supply chain from cradle to gate, including production, harvest, transportation, and utilization. According to the findings, both systems exhibit economic viability, generating sizable profits and having quick payback times. However, despite its larger initial expenditure of EUR 2.74 million, the pyrolysis unit ends up being the better option because it has a payback period of 1.58 years, a return on investment (ROI) of 58% and a net present value (NPV) of EUR 21.5 million. Gasification is still an economically attractive alternative with a lower initial investment (EUR 1.81 million), despite having a lower ROI (36%) and NPV (EUR 10.52 million), as well as a longer payback period (2.41 years). However, the environmental implications of the gasification option are generally higher than those of pyrolysis. The impacts of gasification on fossil depletion (FDP) were estimated to be 5.7 million kg oil eq., compared to 5.3 million kg oil eq. for pyrolysis. Similarly, gasification resulted in 41.55 million kg U235 eq. and pyrolysis in 41.5 million kg U235 eq. related to impacts on ionizing radiation (IRP_HE). Other impact categories that emerge as the most important are freshwater eutrophication (FEP) and marine eutrophication (MEP).
在循环经济中,农业副产品的能源价值化受到高度重视。棉花秆由于能量含量高,适合作为生产生物能源的原料。这项研究的重点领域是每年可气化或热解 25,500 吨棉花秆的系统的经济可行性和对环境的影响。为了进一步了解气化和热解对环境的影响,我们进行了生命周期评估(LCA)。这项分析评估了整个价值链,涵盖了棉花供应链从摇篮到大门的所有阶段,包括生产、收获、运输和利用。分析结果表明,两种系统都具有经济可行性,都能产生可观的利润,而且投资回收期较短。然而,尽管热解装置的初始支出较高,为 274 万欧元,但它最终成为更好的选择,因为它的投资回收期为 1.58 年,投资回报率为 58%,净现值为 2150 万欧元。气化技术虽然投资回报率(36%)和净现值(1052 万欧元)较低,投资回收期(241 年)也较长,但其初始投资较低(181 万欧元),在经济上仍是一种有吸引力的替代方案。不过,气化方案对环境的影响一般高于热解方案。据估计,气化对化石损耗(FDP)的影响为 570 万千克石油当量,而热解为 530 万千克石油当量。同样,在电离辐射影响(IRP_HE)方面,气化产生的铀-235当量为4155万千克,热解产生的铀-235当量为4150万千克。其他最重要的影响类别是淡水富营养化(FEP)和海洋富营养化(MEP)。
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引用次数: 0
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