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Single-cell landscape of immunological responses in elderly patients with sepsis. 老年败血症患者免疫反应的单细胞图谱。
IF 5.2 2区 医学 Q1 GERIATRICS & GERONTOLOGY Pub Date : 2024-06-22 DOI: 10.1186/s12979-024-00446-z
Wanxue He, Chen Yao, Kaifei Wang, Zhimei Duan, Shuo Wang, Lixin Xie

Sepsis is a dysregulated host response to severe infections, and immune dysfunction plays a crucial role in its pathogenesis. Elderly patients, a special population influenced by immunosenescence, are more susceptible to sepsis and have a worse prognosis. However, the immunopathogenic mechanisms underlying sepsis in elderly patients remain unclear. Here, we performed single-cell RNA sequencing of peripheral blood samples from young and old subjects and patients with sepsis. By exploring the transcriptional profiles of immune cells, we analyzed immune cell compositions, phenotype shifts, expression heterogeneities, and intercellular communication. In elderly patients with sepsis, innate immune cells (e.g., monocytes and DCs) exhibit decreased antigen presentation, presenting an overactive inflammatory and senescent phenotype. However, the immunophenotype of T cells shifted to characterize effector, memory, and exhaustion. Moreover, we identified strong interferon-γ responses of T cells in both aging and sepsis groups and a deranged inflammaging status in elderly sepsis patients. Tregs in elderly patients with sepsis showed increased abundance and enhanced immunosuppressive effects. In addition, metabolism-associated pathways were upregulated in T cells in elderly patients with sepsis, and the lysine metabolism pathway was enriched in Tregs. Cell-cell interaction analysis showed that the expression profile of ligand-receptor pairs was probably associated with aggravated immune dysfunction in elderly patients with sepsis. A novel HLA-KIR interaction was observed between Tregs and CD8 + T cells. These findings illustrate the immunological hallmarks of sepsis in elderly patients, and highlight that immunosuppressive and metabolic regulatory pathways may undergo important alterations in elderly patients with sepsis.

败血症是宿主对严重感染的一种失调反应,免疫功能失调在其发病机制中起着至关重要的作用。老年患者是受免疫衰老影响的特殊人群,更易患败血症,且预后较差。然而,老年败血症的免疫致病机制仍不清楚。在此,我们对年轻和老年受试者以及败血症患者的外周血样本进行了单细胞 RNA 测序。通过探索免疫细胞的转录谱,我们分析了免疫细胞的组成、表型转变、表达异质性和细胞间通讯。在老年败血症患者中,先天性免疫细胞(如单核细胞和直流电细胞)的抗原呈递能力下降,呈现出过度活跃的炎症和衰老表型。然而,T 细胞的免疫表型却发生了转变,呈现出效应、记忆和衰竭的特征。此外,我们还发现,在衰老组和败血症组中,T 细胞都有很强的干扰素-γ 反应,而老年败血症患者的炎症状态失常。老年败血症患者的 Tregs 数量增加,免疫抑制作用增强。此外,老年脓毒症患者的 T 细胞中代谢相关通路上调,Tregs 中富含赖氨酸代谢通路。细胞-细胞相互作用分析表明,配体-受体对的表达谱可能与老年脓毒症患者免疫功能障碍加重有关。在Tregs和CD8 + T细胞之间观察到了一种新的HLA-KIR相互作用。这些发现说明了老年败血症患者的免疫学特征,并强调了老年败血症患者的免疫抑制和代谢调节途径可能会发生重要变化。
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引用次数: 0
Kinetics of pro- and anti-inflammatory spike-specific cellular immune responses in long-term care facility residents after COVID-19 mRNA primary and booster vaccination: a prospective longitudinal study in Japan. 日本一项前瞻性纵向研究:COVID-19 mRNA 初次接种和加强接种后长期护理机构居民促炎和抗炎尖峰特异性细胞免疫反应动力学。
IF 5.2 2区 医学 Q1 GERIATRICS & GERONTOLOGY Pub Date : 2024-06-22 DOI: 10.1186/s12979-024-00444-1
Tomoyuki Kakugawa, Yusuke Mimura, Yuka Mimura-Kimura, Keiko Doi, Yuichi Ohteru, Hiroyuki Kakugawa, Keiji Oishi, Masahiro Kakugawa, Tsunahiko Hirano, Kazuto Matsunaga

Background: The magnitude and durability of cell-mediated immunity in older and severely frail individuals following coronavirus disease 2019 (COVID-19) vaccination remain unclear. A controlled immune response could be the key to preventing severe COVID-19; however, it is uncertain whether vaccination induces an anti-inflammatory cellular immune response. To address these issues, a 48-week-long prospective longitudinal study was conducted. A total of 106 infection-naive participants (57 long-term care facility [LTCF] residents [median age; 89.0 years], 28 outpatients [median age; 72.0 years], and 21 healthcare workers [median age; 51.0 years]) provided peripheral blood mononuclear cell (PBMC) samples for the assessment of spike-specific PBMC responses before primary vaccination, 24 weeks after primary vaccination, and three months after booster vaccination. Cellular immune responses to severe acute respiratory syndrome coronavirus 2 spike protein were examined by measuring interferon (IFN)-γ, tumor necrosis factor (TNF), interleukin (IL)-2, IL-4, IL-6, and IL-10 levels secreted from the spike protein peptide-stimulated PBMCs of participants.

Results: LTCF residents exhibited significantly lower IFN-γ, TNF, IL-2, and IL-6 levels than healthcare workers after the primary vaccination. Booster vaccination increased IL-2 and IL-6 levels in LTCF residents comparable to those in healthcare workers, whereas IFN-γ and TNF levels in LTCF residents remained significantly lower than those in healthcare workers. IL-10 levels were not significantly different from the initial values after primary vaccination but increased significantly after booster vaccination in all subgroups. Multivariate analysis showed that age was negatively associated with IFN-γ, TNF, IL-2, and IL-6 levels but not with IL-10 levels. The levels of pro-inflammatory cytokines, including IFN-γ, TNF, IL-2, and IL-6, were positively correlated with humoral immune responses, whereas IL-10 levels were not.

Conclusions: Older and severely frail individuals may exhibit diminished spike-specific PBMC responses following COVID-19 vaccination compared to the general population. A single booster vaccination may not adequately enhance cell-mediated immunity in older and severely frail individuals to a level comparable to that in the general population. Furthermore, booster vaccination may induce not only a pro-inflammatory cellular immune response but also an anti-inflammatory cellular immune response, potentially mitigating detrimental hyperinflammation.

背景:接种2019年冠状病毒病(COVID-19)疫苗后,老年人和严重虚弱者的细胞介导免疫的程度和持久性仍不清楚。受控的免疫反应可能是预防严重 COVID-19 的关键;然而,目前还不确定接种疫苗是否会诱导抗炎细胞免疫反应。为了解决这些问题,我们开展了一项为期 48 周的前瞻性纵向研究。共有 106 名未受感染的参与者(57 名长期护理设施[LTCF]居民[中位年龄;89.0 岁]、28 名门诊患者[中位年龄;72.0 岁]和 21 名医护人员[中位年龄;51.0 岁])提供了外周血单核细胞 (PBMC) 样本,用于评估接种初级疫苗前、初级疫苗接种 24 周后和加强免疫 3 个月后的尖峰特异性 PBMC 反应。通过测量参与者的尖峰蛋白肽刺激的 PBMC 中分泌的干扰素 (IFN)-γ、肿瘤坏死因子 (TNF)、白细胞介素 (IL)-2、IL-4、IL-6 和 IL-10 水平,对严重急性呼吸系统综合征冠状病毒 2 尖峰蛋白的细胞免疫反应进行了检测:结果:接种初级疫苗后,LTCF 居民的 IFN-γ、TNF、IL-2 和 IL-6 水平明显低于医护人员。加强接种使 LTCF 居民的 IL-2 和 IL-6 水平上升,与医护人员相当,而 LTCF 居民的 IFN-γ 和 TNF 水平仍明显低于医护人员。IL-10水平在初次接种后与初始值无明显差异,但在所有亚组中在加强接种后均明显升高。多变量分析显示,年龄与 IFN-γ、TNF、IL-2 和 IL-6 水平呈负相关,但与 IL-10 水平无关。促炎细胞因子(包括 IFN-γ、TNF、IL-2 和 IL-6)的水平与体液免疫反应呈正相关,而 IL-10 的水平则不相关:结论:与普通人群相比,老年人和严重虚弱者接种 COVID-19 疫苗后可能会出现尖峰特异性 PBMC 反应减弱。单次加强免疫可能无法将老年和严重虚弱者的细胞介导免疫力提高到与普通人群相当的水平。此外,加强免疫不仅能诱导促炎症细胞免疫反应,还能诱导抗炎症细胞免疫反应,从而减轻有害的高炎症反应。
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引用次数: 0
Influenza virus infection exacerbates gene expression related to neurocognitive dysfunction in brains of old mice. 流感病毒感染会加剧老年小鼠大脑中与神经认知功能障碍有关的基因表达。
IF 5.2 2区 医学 Q1 GERIATRICS & GERONTOLOGY Pub Date : 2024-06-21 DOI: 10.1186/s12979-024-00447-y
Wenxin Wu, Jeremy S Alexander, J Leland Booth, Craig A Miller, Jordan P Metcalf, Douglas A Drevets

Background: Age > 65 years is a key risk factor for poor outcomes after human influenza infection. Specifically, in addition to respiratory disease, non-neurotropic influenza A virus (IAV) causes neuro-cognitive complications, e.g. new onset depression and increases the risk of dementia after hospitalization. This study aimed to identify potential mechanisms of these effects by determining differences between young and old mice in brain gene expression in a mouse model of non-neurotropic IAV infection.

Methods: Young (12 weeks) and old (70 weeks) C57Bl/6J mice were inoculated intranasally with 200 PFU H1N1 A/PR/34/8 (PR8) or sterile PBS (mock). Gene expression in lung and brain was measured by qRT-PCR and normalized to β-actin. Findings were confirmed using the nCounter Mouse Neuroinflammation Array (NanoString) and analyzed with nSolver 4.0 and Ingenuity Pathway Analysis (IPA, Qiagen).

Results: IAV PR8 did not invade the central nervous system. Young and old mice differed significantly in brain gene expression at baseline and during non-neurotropic IAV infection. Expression of brain Ifnl, Irf7, and Tnf mRNAs was upregulated over baseline control at 3 days post-infection (p.i.) only in young mice, but old mice expressed more Ifnl than young mice 7 days p.i. Gene arrays showed down-regulation of the Epigenetic Regulation, Insulin Signaling, and Neurons and Neurotransmission pathways in old mice 3 days p.i. while young mice demonstrated no change or induction of these pathways at the same time point. IPA revealed marked baseline differences between old and young mice. Gene expression related to Cognitive Impairment, Memory Deficits and Learning worsened in old mice relative to young mice during IAV infection. Aged mice demonstrate more severe changes in gene expression related to memory loss and cognitive dysfunction by IPA.

Conclusions: These data suggest the genes and pathways related to learning and cognitive performance that were worse at baseline in old mice were further worsened by IAV infection, similar to old patients. Early events in the brain triggered by IAV infection portend downstream neurocognitive pathology in old adults.

背景:年龄大于 65 岁是人类感染流感后出现不良后果的关键风险因素。具体而言,除呼吸系统疾病外,非神经性甲型流感病毒(IAV)还会导致神经认知并发症,如新发抑郁症,并增加住院后痴呆的风险。本研究旨在通过确定非神经性甲型流感病毒感染小鼠模型中年轻小鼠和年老小鼠大脑基因表达的差异,找出这些影响的潜在机制:年轻(12 周)和年老(70 周)的 C57Bl/6J 小鼠经鼻接种 200 PFU H1N1 A/PR/34/8 (PR8) 或无菌 PBS(模拟)。通过 qRT-PCR 检测肺部和脑部的基因表达,并与β-肌动蛋白进行归一化。研究结果由 nCounter Mouse Neuroinflammation Array(NanoString)确认,并用 nSolver 4.0 和 Ingenuity Pathway Analysis(IPA,Qiagen)进行分析:结果:IAV PR8没有侵入中枢神经系统。年轻小鼠和年老小鼠在基线和非神经性 IAV 感染期间的脑部基因表达有显著差异。基因阵列显示,感染后3天,老龄小鼠表观遗传调控、胰岛素信号和神经元与神经传导通路下调,而同一时间点,年轻小鼠的这些通路没有变化或诱导。IPA显示了老年小鼠和年轻小鼠之间明显的基线差异。在 IAV 感染期间,老年小鼠与认知障碍、记忆缺陷和学习相关的基因表达比年轻小鼠更差。通过IPA分析,老年小鼠与记忆丧失和认知功能障碍相关的基因表达发生了更严重的变化:这些数据表明,与学习和认知能力相关的基因和通路在基线时在老年小鼠中表现较差,但在感染 IAV 后进一步恶化,这与老年患者的情况类似。IAV感染引发的大脑早期事件预示着老年神经认知病理学的下游发展。
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引用次数: 0
Neuroinflammation in Alzheimer's disease: insights from peripheral immune cells. 阿尔茨海默病的神经炎症:外周免疫细胞的启示。
IF 7.9 2区 医学 Q1 GERIATRICS & GERONTOLOGY Pub Date : 2024-06-14 DOI: 10.1186/s12979-024-00445-0
Qiang Zhang, Guanhu Yang, Yuan Luo, Lai Jiang, Hao Chi, Gang Tian

Alzheimer's disease (AD) is a serious brain disorder characterized by the presence of beta-amyloid plaques, tau pathology, inflammation, neurodegeneration, and cerebrovascular dysfunction. The presence of chronic neuroinflammation, breaches in the blood-brain barrier (BBB), and increased levels of inflammatory mediators are central to the pathogenesis of AD. These factors promote the penetration of immune cells into the brain, potentially exacerbating clinical symptoms and neuronal death in AD patients. While microglia, the resident immune cells of the central nervous system (CNS), play a crucial role in AD, recent evidence suggests the infiltration of cerebral vessels and parenchyma by peripheral immune cells, including neutrophils, T lymphocytes, B lymphocytes, NK cells, and monocytes in AD. These cells participate in the regulation of immunity and inflammation, which is expected to play a huge role in future immunotherapy. Given the crucial role of peripheral immune cells in AD, this article seeks to offer a comprehensive overview of their contributions to neuroinflammation in the disease. Understanding the role of these cells in the neuroinflammatory response is vital for developing new diagnostic markers and therapeutic targets to enhance the diagnosis and treatment of AD patients.

阿尔茨海默病(AD)是一种严重的脑部疾病,其特征是存在β-淀粉样蛋白斑块、tau病理学、炎症、神经变性和脑血管功能障碍。慢性神经炎症的存在、血脑屏障(BBB)的破坏以及炎症介质水平的升高是阿尔茨海默病发病机制的核心。这些因素会促进免疫细胞渗入大脑,可能会加重 AD 患者的临床症状和神经元死亡。小胶质细胞是中枢神经系统(CNS)的常驻免疫细胞,在渐冻症中起着至关重要的作用,而最近的证据表明,在渐冻症中,包括中性粒细胞、T 淋巴细胞、B 淋巴细胞、NK 细胞和单核细胞在内的外周免疫细胞浸润了脑血管和脑实质。这些细胞参与免疫和炎症的调节,预计将在未来的免疫疗法中发挥巨大作用。鉴于外周免疫细胞在AD中的关键作用,本文试图全面概述它们对该病神经炎症的贡献。了解这些细胞在神经炎症反应中的作用,对于开发新的诊断标志物和治疗靶点以提高 AD 患者的诊断和治疗效果至关重要。
{"title":"Neuroinflammation in Alzheimer's disease: insights from peripheral immune cells.","authors":"Qiang Zhang, Guanhu Yang, Yuan Luo, Lai Jiang, Hao Chi, Gang Tian","doi":"10.1186/s12979-024-00445-0","DOIUrl":"10.1186/s12979-024-00445-0","url":null,"abstract":"<p><p>Alzheimer's disease (AD) is a serious brain disorder characterized by the presence of beta-amyloid plaques, tau pathology, inflammation, neurodegeneration, and cerebrovascular dysfunction. The presence of chronic neuroinflammation, breaches in the blood-brain barrier (BBB), and increased levels of inflammatory mediators are central to the pathogenesis of AD. These factors promote the penetration of immune cells into the brain, potentially exacerbating clinical symptoms and neuronal death in AD patients. While microglia, the resident immune cells of the central nervous system (CNS), play a crucial role in AD, recent evidence suggests the infiltration of cerebral vessels and parenchyma by peripheral immune cells, including neutrophils, T lymphocytes, B lymphocytes, NK cells, and monocytes in AD. These cells participate in the regulation of immunity and inflammation, which is expected to play a huge role in future immunotherapy. Given the crucial role of peripheral immune cells in AD, this article seeks to offer a comprehensive overview of their contributions to neuroinflammation in the disease. Understanding the role of these cells in the neuroinflammatory response is vital for developing new diagnostic markers and therapeutic targets to enhance the diagnosis and treatment of AD patients.</p>","PeriodicalId":51289,"journal":{"name":"Immunity & Ageing","volume":"21 1","pages":"38"},"PeriodicalIF":7.9,"publicationDate":"2024-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11177389/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141321985","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Terminally differentiated effector memory T cells associate with cognitive and AD-related biomarkers in an aging-based community cohort. 在一个以老龄化为基础的社区队列中,终末分化的效应记忆 T 细胞与认知和注意力缺失症相关的生物标志物有关。
IF 5.2 2区 医学 Q1 GERIATRICS & GERONTOLOGY Pub Date : 2024-06-12 DOI: 10.1186/s12979-024-00443-2
Edric Winford, Jenny Lutshumba, Barbara J Martin, Donna M Wilcock, Gregory A Jicha, Barbara S Nikolajczyk, Ann M Stowe, Adam D Bachstetter

Background and purpose: The immune response changes during aging and the progression of Alzheimer's disease (AD) and related dementia (ADRD). Terminally differentiated effector memory T cells (called TEMRA) are important during aging and AD due to their cytotoxic phenotype and association with cognitive decline. However, it is not clear if the changes seen in TEMRAs are specific to AD-related cognitive decline specifically or are more generally correlated with cognitive decline. This study aimed to examine whether TEMRAs are associated with cognition and plasma biomarkers of AD, neurodegeneration, and neuroinflammation in a community-based cohort of older adults.

Methods: Study participants from a University of Kentucky Alzheimer's Disease Research Center (UK-ADRC) community-based cohort of aging and dementia were used to test our hypothesis. There were 84 participants, 44 women and 40 men. Participants underwent physical examination, neurological examination, medical history, cognitive testing, and blood collection to determine plasma biomarker levels (Aβ42/Aβ40 ratio, total tau, Neurofilament Light chain (Nf-L), Glial Fibrillary Acidic Protein (GFAP)) and to isolate peripheral blood mononuclear cells (PBMCs). Flow cytometry was used to analyze PBMCs from study participants for effector and memory T cell populations, including CD4+ and CD8+ central memory T cells (TCM), Naïve T cells, effector memory T cells (TEM), and effector memory CD45RA+ T cells (TEMRA) immune cell markers.

Results: CD8+ TEMRAs were positively correlated with Nf-L and GFAP. We found no significant difference in CD8+ TEMRAs based on cognitive scores and no associations between CD8+ TEMRAs and AD-related biomarkers. CD4+ TEMRAs were associated with cognitive impairment on the MMSE. Gender was not associated with TEMRAs, but it did show an association with other T cell populations.

Conclusion: These findings suggest that the accumulation of CD8+ TEMRAs may be a response to neuronal injury (Nf-L) and neuroinflammation (GFAP) during aging or the progression of AD and ADRD. As our findings in a community-based cohort were not clinically-defined AD participants but included all ADRDs, this suggests that TEMRAs may be associated with changes in systemic immune T cell subsets associated with the onset of pathology.

背景和目的:在衰老和阿尔茨海默病(AD)及相关痴呆症(ADRD)进展过程中,免疫反应会发生变化。终末分化的效应记忆 T 细胞(称为 TEMRA)因其细胞毒性表型和与认知能力下降的关系而在衰老和老年痴呆症过程中发挥着重要作用。然而,目前还不清楚TEMRA的变化是专门针对与AD相关的认知能力下降,还是更普遍地与认知能力下降相关。本研究旨在研究 TEMRAs 是否与认知能力以及社区老年人队列中的 AD、神经变性和神经炎症的血浆生物标志物有关:我们利用肯塔基大学阿尔茨海默病研究中心(UK-ADRC)社区老年痴呆症队列中的研究参与者来验证我们的假设。共有 84 名参与者,其中女性 44 人,男性 40 人。参与者接受了体格检查、神经系统检查、病史、认知测试和采血,以确定血浆生物标志物水平(Aβ42/Aβ40比率、总tau、神经丝蛋白轻链(Nf-L)、神经纤维酸性蛋白(GFAP)),并分离外周血单核细胞(PBMC)。流式细胞术用于分析研究参与者外周血单核细胞的效应和记忆 T 细胞群,包括 CD4+ 和 CD8+ 中枢记忆 T 细胞(TCM)、新生 T 细胞、效应记忆 T 细胞(TEM)和效应记忆 CD45RA+ T 细胞(TEMRA)免疫细胞标记:结果:CD8+ TEMRA 与 Nf-L 和 GFAP 呈正相关。我们发现 CD8+ TEMRAs 与认知评分无明显差异,CD8+ TEMRAs 与 AD 相关生物标记物之间也无关联。CD4+ TEMRA 与 MMSE 的认知障碍有关。性别与TEMRAs无关,但与其他T细胞群有关联:这些研究结果表明,CD8+ TEMRAs 的积累可能是对衰老或 AD 和 ADRD 进展过程中神经元损伤(Nf-L)和神经炎症(GFAP)的反应。由于我们在社区队列中的发现并非临床定义的 AD 参与者,而是包括了所有 ADRD,这表明 TEMRAs 可能与病理发作相关的全身免疫 T 细胞亚群的变化有关。
{"title":"Terminally differentiated effector memory T cells associate with cognitive and AD-related biomarkers in an aging-based community cohort.","authors":"Edric Winford, Jenny Lutshumba, Barbara J Martin, Donna M Wilcock, Gregory A Jicha, Barbara S Nikolajczyk, Ann M Stowe, Adam D Bachstetter","doi":"10.1186/s12979-024-00443-2","DOIUrl":"10.1186/s12979-024-00443-2","url":null,"abstract":"<p><strong>Background and purpose: </strong>The immune response changes during aging and the progression of Alzheimer's disease (AD) and related dementia (ADRD). Terminally differentiated effector memory T cells (called T<sub>EMRA</sub>) are important during aging and AD due to their cytotoxic phenotype and association with cognitive decline. However, it is not clear if the changes seen in T<sub>EMRAs</sub> are specific to AD-related cognitive decline specifically or are more generally correlated with cognitive decline. This study aimed to examine whether T<sub>EMRAs</sub> are associated with cognition and plasma biomarkers of AD, neurodegeneration, and neuroinflammation in a community-based cohort of older adults.</p><p><strong>Methods: </strong>Study participants from a University of Kentucky Alzheimer's Disease Research Center (UK-ADRC) community-based cohort of aging and dementia were used to test our hypothesis. There were 84 participants, 44 women and 40 men. Participants underwent physical examination, neurological examination, medical history, cognitive testing, and blood collection to determine plasma biomarker levels (Aβ42/Aβ40 ratio, total tau, Neurofilament Light chain (Nf-L), Glial Fibrillary Acidic Protein (GFAP)) and to isolate peripheral blood mononuclear cells (PBMCs). Flow cytometry was used to analyze PBMCs from study participants for effector and memory T cell populations, including CD4<sup>+</sup> and CD8<sup>+</sup> central memory T cells (T<sub>CM</sub>), Naïve T cells, effector memory T cells (T<sub>EM</sub>), and effector memory CD45RA<sup>+</sup> T cells (T<sub>EMRA</sub>) immune cell markers.</p><p><strong>Results: </strong>CD8<sup>+</sup> T<sub>EMRAs</sub> were positively correlated with Nf-L and GFAP. We found no significant difference in CD8<sup>+</sup> T<sub>EMRAs</sub> based on cognitive scores and no associations between CD8<sup>+</sup> T<sub>EMRAs</sub> and AD-related biomarkers. CD4<sup>+</sup> T<sub>EMRAs</sub> were associated with cognitive impairment on the MMSE. Gender was not associated with T<sub>EMRAs</sub>, but it did show an association with other T cell populations.</p><p><strong>Conclusion: </strong>These findings suggest that the accumulation of CD8<sup>+</sup> T<sub>EMRAs</sub> may be a response to neuronal injury (Nf-L) and neuroinflammation (GFAP) during aging or the progression of AD and ADRD. As our findings in a community-based cohort were not clinically-defined AD participants but included all ADRDs, this suggests that T<sub>EMRAs</sub> may be associated with changes in systemic immune T cell subsets associated with the onset of pathology.</p>","PeriodicalId":51289,"journal":{"name":"Immunity & Ageing","volume":"21 1","pages":"36"},"PeriodicalIF":5.2,"publicationDate":"2024-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11167815/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141312299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Immune cell phenotypes and mortality in the Framingham Heart Study. 弗雷明汉心脏研究中的免疫细胞表型和死亡率。
IF 5.2 2区 医学 Q1 GERIATRICS & GERONTOLOGY Pub Date : 2024-06-12 DOI: 10.1186/s12979-024-00431-6
Ahmed A Y Ragab, Margaret F Doyle, Jiachen Chen, Yuan Fang, Kathryn L Lunetta, Joanne M Murabito

Background: Global life expectancy is rising, with the 60 + age group projected to hit 2 billion by 2050. Aging impacts the immune system. A notable marker of immune system aging is the presence of Aging-Related Immune Cell Phenotypes (ARIPs). Despite their importance, links between immune cell phenotypes including ARIPs and mortality are underexplored. We prospectively investigated 16 different immune cell phenotypes using flow cytometry and IL-6 in relation to survival outcome among dementia-free Framingham Heart Study (FHS) offspring cohort participants who attended the seventh exam (1998-2001).

Results: Among 996 participants (mean age 62 years, range 40 to 88 years, 52% female), the 19-year survival rate was 65%. Adjusting for age, sex, and cytomegalovirus (CMV) serostatus, higher CD4/CD8 and Tc17/CD8 + Treg ratios were significantly associated with lower all-cause mortality (HR: 0.86 [0.76-0.96], 0.84 [0.74-0.94], respectively), while higher CD8 regulatory cell levels (CD8 + CD25 + FoxP3 +) were associated with increased all-cause mortality risk (HR = 1.17, [1.03-1.32]). Elevated IL-6 levels correlated with higher all-cause, cardiovascular, and non-cardiovascular mortality (HR = 1.43 [1.26-1.62], 1.70 [1.31-2.21], and 1.36 [1.18-1.57], respectively). However, after adjusting for cardiovascular risk factors and prevalent cancer alongside age, sex, and CMV, immune cell phenotypes were no longer associated with mortality in our cohort. Nonetheless, IL-6 remained significantly associated with all-cause and cardiovascular mortality (HRs: 1.3 [1.13-1.49], 1.5 [1.12-1.99], respectively).

Conclusions: In 19-year follow-up, higher Tc17/CD8 + Treg and CD4/CD8 ratios were associated with lower all-cause mortality, while the CD8 + CD25 + FoxP3 + (CD8 + Treg) phenotype showed increased risk. Elevated IL-6 levels consistently correlated with amplified mortality risks. These findings highlight the links between immune phenotypes and mortality, suggesting implications for future research and clinical considerations.

背景:全球人口的预期寿命正在延长,预计到 2050 年,60 岁以上的人口将达到 20 亿。衰老会影响免疫系统。免疫系统衰老的一个显著标志是出现与衰老相关的免疫细胞表型(ARIPs)。尽管ARIPs非常重要,但包括ARIPs在内的免疫细胞表型与死亡率之间的联系却未得到充分探索。我们使用流式细胞仪和 IL-6 对参加第七次检查(1998-2001 年)的无痴呆症的弗雷明汉心脏研究(FHS)后代队列参与者中 16 种不同的免疫细胞表型与生存结果的关系进行了前瞻性研究:在996名参与者(平均年龄62岁,年龄范围40-88岁,52%为女性)中,19年存活率为65%。调整年龄、性别和巨细胞病毒(CMV)血清状态后,CD4/CD8 和 Tc17/CD8 + Treg 比率越高,全因死亡率越低(HR:分别为 0.86 [0.76-0.96]、0.84 [0.74-0.94]),而 CD8 调节细胞水平越高(CD8 + CD25 + FoxP3 +),全因死亡风险越高(HR = 1.17,[1.03-1.32])。IL-6 水平升高与全因、心血管和非心血管死亡率升高相关(HR = 1.43 [1.26-1.62]、1.70 [1.31-2.21] 和 1.36 [1.18-1.57])。然而,在调整了心血管风险因素和流行性癌症以及年龄、性别和 CMV 后,我们队列中的免疫细胞表型不再与死亡率相关。然而,IL-6仍与全因死亡率和心血管死亡率显著相关(HRs:分别为1.3 [1.13-1.49]、1.5 [1.12-1.99]):在19年的随访中,较高的Tc17/CD8 + Treg和CD4/CD8比率与较低的全因死亡率相关,而CD8 + CD25 + FoxP3 + (CD8 + Treg)表型则显示风险增加。IL-6水平的升高与死亡率风险的增加一直相关。这些发现凸显了免疫表型与死亡率之间的联系,为今后的研究和临床考虑提供了参考。
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引用次数: 0
Activating A1 adenosine receptor signaling boosts early pulmonary neutrophil recruitment in aged mice in response to Streptococcus pneumoniae infection 激活 A1 腺苷受体信号可促进老龄小鼠在肺炎链球菌感染时早期肺中性粒细胞的募集
IF 7.9 2区 医学 Q1 GERIATRICS & GERONTOLOGY Pub Date : 2024-06-05 DOI: 10.1186/s12979-024-00442-3
Shaunna R. Simmons, Sydney E. Herring, Essi Y.I Tchalla, Alexsandra P. Lenhard, Manmeet Bhalla, Elsa N. Bou Ghanem
Streptococcus pneumoniae (pneumococcus) is a leading cause of pneumonia in older adults. Successful control of pneumococci requires robust pulmonary neutrophil influx early in infection. However, aging is associated with aberrant neutrophil recruitment and the mechanisms behind that are not understood. Here we explored how neutrophil recruitment following pneumococcal infection changes with age and the host pathways regulating this. Following pneumococcal infection there was a significant delay in early neutrophil recruitment to the lungs of aged mice. Neutrophils from aged mice showed defects in trans-endothelial migration in vitro compared to young controls. To understand the pathways involved, we examined immune modulatory extracellular adenosine (EAD) signaling, that is activated upon cellular damage. Signaling through the lower affinity A2A and A2B adenosine receptors had no effect on neutrophil recruitment to infected lungs. In contrast, inhibition of the high affinity A1 receptor in young mice blunted neutrophil recruitment to the lungs following infection. A1 receptor inhibition decreased expression of CXCR2 on circulating neutrophils, which is required for trans-endothelial migration. Indeed, A1 receptor signaling on neutrophils was required for their ability to migrate across endothelial cells in response to infection. Aging was not associated with defects in EAD production or receptor expression on neutrophils. However, agonism of A1 receptor in aged mice rescued the early defect in neutrophil migration to the lungs and improved control of bacterial burden. This study suggests age-driven defects in EAD damage signaling can be targeted to rescue the delay in pulmonary neutrophil migration in response to bacterial pneumonia.
肺炎链球菌(肺炎球菌)是导致老年人肺炎的主要原因。要成功控制肺炎球菌,就必须在感染早期让肺部中性粒细胞大量涌入。然而,衰老与中性粒细胞异常招募有关,其背后的机制尚不清楚。在这里,我们探讨了肺炎球菌感染后中性粒细胞的招募是如何随着年龄的增长而发生变化的,以及调节这一变化的宿主途径。肺炎球菌感染后,老年小鼠肺部的早期中性粒细胞募集明显延迟。与年轻对照组相比,老年小鼠的中性粒细胞在体外跨内皮迁移方面表现出缺陷。为了了解其中涉及的途径,我们研究了细胞外腺苷(EAD)信号的免疫调节作用。通过低亲和力 A2A 和 A2B 腺苷受体发出的信号对中性粒细胞招募到受感染肺部没有影响。相反,在幼鼠体内抑制高亲和力的 A1 受体则会阻碍中性粒细胞在感染后被招募到肺部。A1受体抑制降低了循环中性粒细胞上CXCR2的表达,而CXCR2是跨内皮迁移所必需的。事实上,嗜中性粒细胞上的A1受体信号传导是嗜中性粒细胞在感染时跨内皮细胞迁移的必要条件。衰老与中性粒细胞上 EAD 的产生或受体表达的缺陷无关。然而,激动老龄小鼠的 A1 受体可挽救中性粒细胞向肺部迁移的早期缺陷,并改善对细菌负荷的控制。这项研究表明,可以针对年龄驱动的EAD损伤信号传导缺陷来挽救中性粒细胞在应对细菌性肺炎时向肺部迁移的延迟。
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引用次数: 0
The role of blood related inflammatory factors on age-related macular degeneration (AMD) 血液相关炎症因子对老年性黄斑变性(AMD)的作用
IF 7.9 2区 医学 Q1 GERIATRICS & GERONTOLOGY Pub Date : 2024-06-05 DOI: 10.1186/s12979-024-00440-5
Habib Ojaghi, Shirin Poorsheykhian, Amin Najafi, Sohrab Iranpour
Age-related macular degeneration (AMD) is a significant retinal disease that leads to irreversible low vision, particularly in developing countries. The variation in AMD prevalence among different racial groups and highlighted role of inflammation on disease pathology from previous studies which yielded in inconsistent findings, It seems to be of great importance to do more investigation in this field. This case control study involved 204 participants, divided into four groups of equal size (51 individuals per group). Three groups represented AMD cases of varying severity according to Beckman classification (3 groups) and one healthy control group. Sampling was conducted exhaustively until the desired sample size was reached. The control group comprised healthy individuals without any infectious or inflammatory systemic, ophthalmic disease. Blood samples were collected to measure inflammatory factors, including lymphocytes, monocytes, neutrophils, neutrophil-to-lymphocyte ratio (NLR), and C-reactive protein (CRP). Collected data were analyzed by statistical methods in SPSS version 21. Of the participants, 51% were women, and their ages ranged from 47 to 89 years (62.2 ± 8). According to multiple logistic regression analysis, age exhibited a statistically significant positive association with AMD severity (P = 0.038, odds ratio [OR] = 1.034). ANOVA results indicated a significant association between neutrophil count and AMD severity (P < 0.001). As the disease severity increased, the number of neutrophils decreased. The mean ± SD neutrophil counts for early, intermediate and advanced AMD were 3849 ± 800, 3702 ± 734, and 3342 ± 823, respectively. No statistically significant associations were found between lymphocyte count, monocyte count, neutrophil-to-lymphocyte ratio, CRP, and AMD. There was a significant relationship between the number of neutrophils in peripheral blood and the severity of AMD in study participants which needs more evaluation for the potential utility of this factor in the prognosis of AMD. There was not any significant relationship among the other factors and AMD.
老年性黄斑变性(AMD)是一种严重的视网膜疾病,会导致不可逆的低视力,尤其是在发展中国家。由于不同种族群体的老年黄斑变性发病率存在差异,而且以往的研究强调炎症对疾病病理的作用,但得出的结论并不一致,因此在这一领域开展更多的调查似乎非常重要。这项病例对照研究涉及 204 名参与者,分为四组,每组 51 人,每组人数相等。根据贝克曼分类法,三组代表不同严重程度的 AMD 病例(3 组),另一组为健康对照组。抽样工作一直持续到达到所需的样本量为止。对照组由没有任何感染性或炎症性系统性眼科疾病的健康人组成。采集的血液样本用于测量炎症因子,包括淋巴细胞、单核细胞、中性粒细胞、中性粒细胞与淋巴细胞比值(NLR)和 C 反应蛋白(CRP)。收集的数据采用 SPSS 21 版的统计方法进行分析。在参与者中,51%为女性,年龄在47至89岁之间(62.2 ± 8)。根据多元逻辑回归分析,年龄与 AMD 严重程度呈显著正相关(P = 0.038,几率比 [OR] = 1.034)。方差分析结果表明,中性粒细胞计数与 AMD 严重程度有明显的相关性(P < 0.001)。随着疾病严重程度的增加,中性粒细胞的数量也随之减少。早期、中期和晚期AMD中性粒细胞计数的平均值(±SD)分别为3849±800、3702±734和3342±823。淋巴细胞计数、单核细胞计数、中性粒细胞与淋巴细胞比率、CRP与AMD之间没有统计学意义上的关联。研究参与者外周血中的中性粒细胞数量与老年性黄斑病变的严重程度有明显关系,需要进一步评估这一因素在老年性黄斑病变预后中的潜在作用。其他因素与老年性黄斑病变之间没有明显关系。
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引用次数: 0
Chemokine receptor 7 contributes to T- and B-cell filtering in ageing bladder, cystitis and bladder cancer. 趋化因子受体 7 对老化膀胱、膀胱炎和膀胱癌中的 T 细胞和 B 细胞过滤有促进作用。
IF 7.9 2区 医学 Q1 GERIATRICS & GERONTOLOGY Pub Date : 2024-05-18 DOI: 10.1186/s12979-024-00432-5
Jiang Zhao, Xing Luo, Chengfei Yang, Xiao Yang, Min Deng, Bishao Sun, Jingzhen Zhu, Zongming Dong, Yangcai Wang, Jia Li, Xingliang Yang, Benyi Li, Xiangwei Wang, Ji Zheng

Background: Research has suggested significant correlations among ageing, immune microenvironment, inflammation and tumours. However, the relationships among ageing, immune microenvironment, cystitis and bladder urothelial carcinoma (BLCA) in the bladder have rarely been reported.

Methods: Bladder single-cell and transcriptomic data from young and old mice were used for immune landscape analysis. Transcriptome, single-cell and The Cancer Genome Atlas Program datasets of BLCA and interstitial cystitis/bladder pain syndrome (IC/BPS) were used to analyse immune cell infiltration and molecular expression. Bladder tissues from mice, IC/BPS and BLCA were collected to validate the results.

Results: Eight types of immune cells (macrophages, B-cells, dendritic cells, T-cells, monocytes, natural killer cells, γδ T-cells and ILC2) were identified in the bladder of mice. Aged mice bladder tissues had a significantly higher number of T-cells, γδ T-cells, ILC2 and B-cells than those in the young group (P < 0.05). Three types of T-cells (NK T-cells, γδ T-cells and naïve T-cells) and three types of B-cells (follicular B-cells, plasma and memory B-cells) were identified in aged mice bladder. Chemokine receptor 7 (CCR7) is highly expressed in aged bladder, IC/BPS and BLCA (P < 0.05). CCR7 is likely to be involved in T- and B-cell infiltration in aged bladder, IC/BPS and BLCA. Interestingly, the high CCR7 expression on BLCA cell membranes was a prognostic protective factor.

Conclusions: In this study, we characterised the expression profiles of immune cells in bladder tissues of aged and young mice and demonstrated that CCR7-mediated T- and B-cell filtration contributes to the development of bladder ageing, IC/BPS and BLCA.

背景:研究表明,衰老、免疫微环境、炎症和肿瘤之间存在明显的相关性。然而,有关膀胱衰老、免疫微环境、膀胱炎和膀胱尿路上皮癌(BLCA)之间关系的研究却鲜有报道:方法:利用年轻小鼠和老年小鼠的膀胱单细胞和转录组数据进行免疫景观分析。利用BLCA和间质性膀胱炎/膀胱疼痛综合征(IC/BPS)的转录组、单细胞和癌症基因组图谱计划数据集分析免疫细胞浸润和分子表达。收集了小鼠、IC/BPS 和 BLCA 的膀胱组织来验证结果:结果:在小鼠膀胱中发现了八种类型的免疫细胞(巨噬细胞、B 细胞、树突状细胞、T 细胞、单核细胞、自然杀伤细胞、γδ T 细胞和 ILC2)。老龄小鼠膀胱组织中的 T 细胞、γδ T 细胞、ILC2 和 B 细胞数量明显高于年轻组(P 结论:老龄小鼠膀胱组织中的 T 细胞、γδ T 细胞、ILC2 和 B 细胞数量明显高于年轻组(P):在这项研究中,我们分析了老龄小鼠和年轻小鼠膀胱组织中免疫细胞的表达谱,并证明了 CCR7 介导的 T 细胞和 B 细胞滤过是膀胱老化、IC/BPS 和 BLCA 发展的原因。
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引用次数: 0
The neutrophil to lymphocyte ratio associates with markers of Alzheimer's disease pathology in cognitively unimpaired elderly people. 中性粒细胞与淋巴细胞的比率与认知能力未受损的老年人阿尔茨海默病病理标志物有关。
IF 7.9 2区 医学 Q1 GERIATRICS & GERONTOLOGY Pub Date : 2024-05-17 DOI: 10.1186/s12979-024-00435-2
Tovia Jacobs, Sean R Jacobson, Juan Fortea, Jeffrey S Berger, Alok Vedvyas, Karyn Marsh, Tianshe He, Eugenio Gutierrez-Jimenez, Nathanael R Fillmore, Moses Gonzalez, Luisa Figueredo, Naomi L Gaggi, Chelsea Reichert Plaska, Nunzio Pomara, Esther Blessing, Rebecca Betensky, Henry Rusinek, Henrik Zetterberg, Kaj Blennow, Lidia Glodzik, Thomas M Wisniweski, Mony J de Leon, Ricardo S Osorio, Jaime Ramos-Cejudo

Background: An elevated neutrophil-lymphocyte ratio (NLR) in blood has been associated with Alzheimer's disease (AD). However, an elevated NLR has also been implicated in many other conditions that are risk factors for AD, prompting investigation into whether the NLR is directly linked with AD pathology or a result of underlying comorbidities. Herein, we explored the relationship between the NLR and AD biomarkers in the cerebrospinal fluid (CSF) of cognitively unimpaired (CU) subjects. Adjusting for sociodemographics, APOE4, and common comorbidities, we investigated these associations in two cohorts: the Alzheimer's Disease Neuroimaging Initiative (ADNI) and the M.J. de Leon CSF repository at NYU. Specifically, we examined associations between the NLR and cross-sectional measures of amyloid-β42 (Aβ42), total tau (t-tau), and phosphorylated tau181 (p-tau), as well as the trajectories of these CSF measures obtained longitudinally.

Results: A total of 111 ADNI and 190 NYU participants classified as CU with available NLR, CSF, and covariate data were included. Compared to NYU, ADNI participants were older (73.79 vs. 61.53, p < 0.001), had a higher proportion of males (49.5% vs. 36.8%, p = 0.042), higher BMIs (27.94 vs. 25.79, p < 0.001), higher prevalence of hypertensive history (47.7% vs. 16.3%, p < 0.001), and a greater percentage of Aβ-positivity (34.2% vs. 20.0%, p = 0.009). In the ADNI cohort, we found cross-sectional associations between the NLR and CSF Aβ42 (β = -12.193, p = 0.021), but not t-tau or p-tau. In the NYU cohort, we found cross-sectional associations between the NLR and CSF t-tau (β = 26.812, p = 0.019) and p-tau (β = 3.441, p = 0.015), but not Aβ42. In the NYU cohort alone, subjects classified as Aβ + (n = 38) displayed a stronger association between the NLR and t-tau (β = 100.476, p = 0.037) compared to Aβ- subjects or the non-stratified cohort. In both cohorts, the same associations observed in the cross-sectional analyses were observed after incorporating longitudinal CSF data.

Conclusions: We report associations between the NLR and Aβ42 in the older ADNI cohort, and between the NLR and t-tau and p-tau in the younger NYU cohort. Associations persisted after adjusting for comorbidities, suggesting a direct link between the NLR and AD. However, changes in associations between the NLR and specific AD biomarkers may occur as part of immunosenescence.

背景:血液中中性粒细胞-淋巴细胞比率(NLR)升高与阿尔茨海默病(AD)有关。然而,NLR 升高也与许多其他疾病有关,而这些疾病都是导致阿尔茨海默病的风险因素,这促使人们研究 NLR 是直接与阿尔茨海默病的病理变化有关,还是潜在合并症的结果。在此,我们探讨了 NLR 与认知功能未受损(CU)受试者脑脊液(CSF)中的 AD 生物标志物之间的关系。在对社会人口统计学、APOE4 和常见合并症进行调整后,我们在两个队列中研究了这些关联:阿尔茨海默病神经影像学倡议(ADNI)和纽约大学的 M.J. de Leon CSF 储存库。具体来说,我们研究了NLR与淀粉样蛋白-β42(Aβ42)、总tau(t-tau)和磷酸化tau181(p-tau)的横断面测量值之间的关系,以及纵向获得的这些CSF测量值的轨迹:共纳入111名ADNI参与者和190名纽约大学参与者,他们被归类为CU,并提供了NLR、CSF和协变量数据。与纽约大学相比,ADNI 参与者的年龄更大(73.79 岁对 61.53 岁,P 结论:ADNI 参与者的年龄与 NLR 和 CSF 的相关性更大:我们报告了年龄较大的 ADNI 群体中 NLR 与 Aβ42 之间的关系,以及较年轻的纽约大学群体中 NLR 与 t-tau 和 p-tau 之间的关系。在对合并症进行调整后,两者之间的关系依然存在,这表明 NLR 与 AD 之间存在直接联系。不过,NLR与特定AD生物标志物之间的关联变化可能是免疫衰老的一部分。
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Immunity & Ageing
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