No other studies have investigated the effect of cognitive behavioral therapy on discrimination, attitudes, empathy, and body perception toward older adults. The study aimed to investigate the effect of cognitive behavioral therapy on discrimination, attitudes, empathy, and body perception in older care students. A single-blind randomized controlled trial was conducted with a total of 53 older adult care students. Participants were randomized to the Cognitive Behavioral Therapy Group (CBTG) (n: 25) and Control Group (CG) (n: 28). Participants in the CBTG group received a four-module training on discrimination, attitudes, empathy, and body perception in terms of older care. Individuals were assessed before and after the intervention with the Ageism Attitude Scale (ADAS), the Kogan Scale of Attitudes Toward Older People (KAOP), and the Children’s Cognitive, Affective and Somatic Empathy Scales (CASES). A significant difference was observed between the two groups regarding the KAOP score (p < 0.05). The KAOP score of CBTG increased significantly after the intervention (p < 0.05). In addition, a significant improvement was observed in the ADAS (Negative discrimination towards older adults) score of the individuals in the intervention group (p < 0.05). The ADAS-negative discrimination score of the individuals in the CBTG group was higher than the control group (p < 0.05). Cognitive-behavioral training improved students’ attitudes toward older adults and negative discrimination. CBT is an effective method for improving discrimination and attitudes towards older adults.