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Sensitivity of Preliminary Blood Test on Various Floor Surfaces After Washing with Different Cleansing Products. : Original Work 用不同清洁产品清洗各种地板表面后进行初步血液检测的灵敏度:原创作品
Pub Date : 2024-01-18 DOI: 10.37506/5ftcva91
Oshin Hathi, Lav Kesharwani, M. Mishra
Blood is important evidence that can assist an investigator in solving a crime. It connects the suspect to a crime and aid in the reconstruction of the crime scene. Criminals frequently attempt to wipe away blood stained evidence at a crime scene. These efforts may result in the modification or partial or total elimination of blood on the stained parts. Numerous presumptive tests are employed to identify bloodstains on floor surfaces since they are frequently cleansed after a crime using various surfactants. However, the traces of stains remain there and if analysed with appropriate reagents will give conclusive results. In This study Blood-stained floor surfaces were cleaned using a commercially available and widely used floor cleaners and After repeated washings, the Kastle-Meyer (KM),Leucomalachite green (LMG), and Tetra methylbenzidine (TMB) tests were employed to determine the presence of blood on these surfaces, and their sensitivity were assessed, this study will help the forensic investigator to select the appropriate reagents for detection of blood stains on the various washed floor surfaces and effectiveness of reagents for detection of blood stains washed with various floor cleaners.
血液是协助调查人员破案的重要证据。它可以将犯罪嫌疑人与犯罪联系起来,并帮助重建犯罪现场。罪犯经常试图擦去犯罪现场沾有血迹的证据。这些努力可能会改变或部分或全部消除沾有血迹的部分。由于地板表面在案发后经常使用各种表面活性剂进行清洁,因此采用了大量的推定测试来识别地板表面的血迹。但是,血迹的痕迹仍然存在,如果使用适当的试剂进行分析,就会得出确定的结果。在这项研究中,使用市面上广泛使用的地板清洁剂对沾有血迹的地板表面进行清洁,经过反复清洗后,使用 Kastle-Meyer (KM)、白孔雀石绿 (LMG) 和四甲基联苯胺 (TMB) 测试来确定这些表面是否存在血迹,并对其灵敏度进行了评估,这项研究将有助于法医调查人员选择适当的试剂来检测各种清洗过的地板表面上的血迹,以及检测使用各种地板清洁剂清洗过的血迹的试剂的有效性。
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引用次数: 0
Pattern, Severity of Injuires Sustained in Road Traffic Accident: An Autopsy Based Study 道路交通事故中受伤的模式和严重程度:基于尸检的研究
Pub Date : 2024-01-18 DOI: 10.37506/4nykex56
Sadasivam Shanmugam¹, Swarana Sekar², Kaviya Kamalanathan³, Swarana Sekar, Junior Resident
Road Traffic Accidents (RTA’s) constitute a significant public health problem, they are rising in an alarmingrate and causing increased morbidity and mortality among people. Motor vehicle accidents are complex events resulting primarily from human, technical, and environmental contributing factors. That is the reason, road traffic accidents are creating the burden for people related to mortality and morbidity.Objectives: The following study analyses the following: 1) Age and sex distribution of the deceased in RTA. 2)Month and Time of occurrence of RTA. 3) Pattern of injury sustained in RTA.Methodology: A retrospective study was conducted for a period of three month (October 2022-December 2022), in cases of road traffic accidents brought for autopsy at tertiary care teaching hospital, Madurai. All the data regarding the age, sex, month, time and place of occurrence, type and site of injuries and outcome are collected based on autopsy report. Data was analysed using standard statistical method.Results: Of the total 330 road traffic accidents cases, 81.8% cases were males, 18.2% females. Majority of the victims were in the age group of 41-50 years (21%), Urban victims found to be on the highest side (77.2%), compared to Rural victims 22.8%. Most of the RTA happened during the month of NOVEMBER (38.2%) and during the time period between 2 PM and 1 0PM (40.6%). Head injuries contributes the most common injury pattern (60.7%) compared to other pattern of injury.Conclusion: The present study concluded that head injuries were the most common injury pattern in RTAs and it is the main factor for death. Proper awareness about usage of helmets, punishment regarding drunk & driving and rash driving among the common people will help to reduce the mortality due to Road traffic accidents.
道路交通事故(RTA)是一个重大的公共卫生问题,其上升速度令人担忧,并导致人们的发病率和死亡率上升。机动车事故是一种复杂的事件,主要由人为、技术和环境因素造成。正因为如此,道路交通事故给人们带来了死亡率和发病率方面的负担:以下研究分析了以下内容:1)道路交通事故中死者的年龄和性别分布。2)道路交通事故发生的月份和时间。3)在道路交通意外中受伤的模式:在为期三个月(2022 年 10 月至 2022 年 12 月)的时间内,对马杜赖三级教学医院送检的道路交通事故案例进行了回顾性研究。根据尸检报告收集了有关年龄、性别、月份、发生时间和地点、受伤类型和部位以及结果的所有数据。数据采用标准统计方法进行分析:在总共 330 例道路交通事故中,男性占 81.8%,女性占 18.2%。大多数受害者的年龄在 41-50 岁之间(21%),城市受害者的比例最高(77.2%),而农村受害者的比例为 22.8%。大多数交通事故发生在 11 月(38.2%)和下午 2 点至 10 点之间(40.6%)。与其他受伤模式相比,头部受伤是最常见的受伤模式(60.7%):本研究得出结论,头部受伤是道路交通意外中最常见的受伤模式,也是导致死亡的主要因素。在普通人中适当宣传使用头盔、对酒后驾驶和鲁莽驾驶进行处罚,将有助于降低道路交通事故造成的死亡率。
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引用次数: 0
Artificial Intelligence and its Role in Forensic Karyotyping: A Systematic Review 人工智能及其在法医核型分析中的作用:系统回顾
Pub Date : 2024-01-18 DOI: 10.37506/900g7477
Sachin Kumar Tripathi, Khyati Rao, Rajiv Ratan Singh, Pradeep kumar Yadav
Introduction: One of the most important aspects of forensic investigations and genetic research is forensickaryotyping, which involves analyzing a person’s chromosomes to find genetic anomalies and establishidentification. The development of artificial intelligence (AI) technology offers a chance to improve and automate the forensic karyotyping procedure. This study examines the possible advantages and difficulties of artificial intelligence (AI) in forensic karyotyping. In forensic science, forensic karyotyping is essential for providing an accurate interpretation of genetic data for use in legal and investigative processes. It can offer  useful details regarding genetic problems, such as chromosomal abnormalities or mutations, which can help with personal identification, paternity determination, or supplying proof in criminal investigations.Aim: To give a general review of how artificial intelligence is used in forensic karyotyping, evaluate its possible advantages, and address any relevant issues. By being aware of Artificial Intelligence’s promise and limitations in this field, we may set the stage for its efficient integration into forensic practitioner.Methods: A database search we did to start the inquiry turned up 582 documents. There were 216 unique records left after duplicates were eliminated. 232 items were subsequently eliminated as a result of download problems. A final sample of 31 research was chosen from the 134 full-text papers that were evaluated (n=134), with 103 being eliminated owing to quality issues.Result: The use of artificial intelligence (AI) in forensic karyotyping has several advantages, including automated chromosomal analysis, quicker abnormality discovery, and increased uniformity. For a successful application, challenges such as a lack of labelled datasets and ethical issues must be resolved.Concussion: By increasing productivity, precision, and uniformity, artificial intelligence has the potential totransform forensic karyotyping. While there are obstacles, continued study and cooperation amongst several fields might help you get through them. The ethical and appropriate use of AI in forensic karyotyping will improve forensic investigations, boost genetic research, and expand the use of genetics in the legal system.
导言:法医核型分析是法医调查和基因研究中最重要的环节之一,它涉及分析一个人的染色体,以发现基因异常并确定身份。人工智能(AI)技术的发展为法医核型分析程序的改进和自动化提供了机会。本研究探讨了人工智能(AI)在法医核型分析中可能存在的优势和困难。在法医学中,法医核型分析对于准确解释用于法律和调查过程的基因数据至关重要。它可以提供有关遗传问题(如染色体异常或突变)的有用细节,有助于个人身份鉴定、亲子鉴定或在刑事调查中提供证据。目的:对人工智能在法医核型分析中的应用进行总体回顾,评估其可能的优势,并解决任何相关问题。通过了解人工智能在这一领域的前景和局限性,我们可以为人工智能与法医从业人员的有效结合创造条件:我们在开始调查时进行了一次数据库搜索,共搜索到 582 份文件。除去重复的记录后,还剩下 216 条唯一记录。由于下载问题,随后又删除了 232 条。最后从评估的 134 篇全文论文(n=134)中选取了 31 篇研究样本,其中 103 篇因质量问题被剔除:在法医核型分析中使用人工智能(AI)有几个优点,包括自动染色体分析、更快地发现异常和提高统一性。要想成功应用,必须解决缺乏标记数据集和伦理问题等挑战:通过提高生产力、精确度和统一性,人工智能有可能改变法医核型分析。虽然存在障碍,但多个领域之间的持续研究与合作可能会帮助您克服这些障碍。在法医核型分析中合理、合乎道德地使用人工智能将改善法医调查,促进遗传学研究,并扩大遗传学在法律系统中的应用。
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引用次数: 0
Comparative Evaluation of Odontometric Parameters for Determining Gender 用于确定性别的牙齿测量参数比较评估
Pub Date : 2024-01-18 DOI: 10.37506/vn6q6e33
R. Bhoot, Vanrajsinh K Rathva
Background and Aim: Sex estimate is a crucial component of human identification in a forensic setting. Due to the sexual dimorphism of human teeth, odontometrics—the measuring and analysis of tooth size standards—is most frequently utilized in age and sex determination. The aim of this study was to assess the degree of sexualdimorphism in mandibular canine using mandibular canine index using mesiodistal (MD) and buccolingual (BL)dimensions of the crown and inter canine distance measured.Material and Methods: The study sample included 200 people (100 men and 100 women), ages 18 to 35, withan average age of 22 years. The distance between the mesial and distal contact points was used to calculate the mesiodistal dimension of the mandibular canine (CMD). Each subject’s right mandibular canine index and left mandibular canine index were used to construct the canine index. The standard canine index (SCI) value was used as a cutoff point to differentiate males from females.Results: Men’s right and left mandibular canines mesio-distal crown widths were found to be significantly greater than those of their female counterparts. The current study demonstrates a very high significance of p value seen in the left mandibular canine and a significant difference in mesiodistal breadth between males and females of the right mandibular canine of p value. The total probability of sex determination for the right mandibular canine is 55% and for the left canine is 59.5% based on the conventional MCI calculations for the right mandibular canine (0.22) and for the left mandibular canine (0.21), suggesting that the left mandibular canine is more relevant for sex determination.Conclusion: It has been proven beyond a reasonable doubt that mandibular canines exhibit significant sexual dimorphism when it comes to sex determination, and it is clear from the study that this breed is a more trustworthy source for sex determination.
背景和目的:在法医鉴定中,性别估计是人类身份鉴定的重要组成部分。由于人类牙齿的性二形性,牙体测量学--牙齿尺寸标准的测量和分析--最常用于年龄和性别鉴定。本研究的目的是使用下颌犬齿指数评估下颌犬齿的性别二形性程度,该指数使用牙冠的牙间距(MD)和颊舌侧(BL)尺寸以及测量的犬齿间距:研究样本包括 200 人(100 名男性和 100 名女性),年龄在 18 至 35 岁之间,平均年龄为 22 岁。下颌犬齿的牙冠中径尺寸(CMD)是用中端和远端接触点之间的距离来计算的。每个受试者的右下颌犬齿指数和左下颌犬齿指数用于构建犬齿指数。以标准犬齿指数(SCI)值作为区分男性和女性的分界点:结果:发现男性的左右下颌犬齿中远牙冠宽度明显大于女性。目前的研究表明,左下颌犬齿的 p 值非常显著,而右下颌犬齿的男性和女性牙冠中远径宽度的 p 值差异显著。根据传统的 MCI 计算,右下颌犬齿(0.22)和左下颌犬齿(0.21)的性别鉴定总概率分别为 55%和 59.5%,这表明左下颌犬齿与性别鉴定更为相关:毫无疑问,下颌犬齿在性别鉴定方面表现出明显的性二态性,而且从研究中可以清楚地看出,该品种在性别鉴定方面更值得信赖。
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引用次数: 0
Assessment of Applicability of Gleurich and Pyle (GP) Method for Determination of Age of Children in 14-18 Years Age-Group at S.M.S. Medical College, Jaipur 在斋浦尔 S.M.S. 医学院评估 Gleurich 和 Pyle (GP) 法在确定 14-18 岁年龄组儿童年龄方面的适用性
Pub Date : 2024-01-18 DOI: 10.37506/3kqdxz10
Ashish Kumar, Sumanta Dutta, Manish Kumar Dewat, Narendra Sisodia
Background, Aim and Objective: Age estimation is also one of the important factors to establish individual’sidentity and radiological investigations play a phenomenal role in estimation of age in conjunction with clinical examination. The aim of this study to assess the applicability of Greulich and Pyle (GP) method for determination of age in 14-18 years of age group healthy population of Jaipur region. And objective is to assess the skeletal age determined by GP method using hand radiography of study population.Method: This study was carried out on total 80 subjects at the Department of Forensic Medicine in association with the Department of Radiology after obtaining due permission and approval from the RRB and IEC of SMS Medical College, Jaipur during the period from 1st July, 2020 to 31st August, 2021 of subjects between 14-18 years of age. All subjects recruited from each age sub-group category viz 14-15 years (Group A), 15-16 years (Group B), 16-17 years (Group C) and 17-18 years (Group D) including equal number of members from both sexes (10 males and 10 females of each age sub group category). After obtaining valid informed consent for age estimation all details,general physical & dental examination was done and recorded in proposed Pro forma. The skeletal age (bone age) was determined using Greulich and Pyle Atlas (2nd edition, 1959)15. X-ray image on computer was comparedwith images on GP Atlas according to sex and most matching image to assess the skeletal age. Images wereseparated in two groups according to their sex and examined by both observers at different occasions to determine skeletal age of subjects and result derived.Result: Among all (n=80), of 81.2% Hindus (34 males and 31 females), 12.5% Muslims (4 males and 6 females) and 6.25% Jains (2 males and 3 females). Correlation of estimated skeletal age by GP method to the chronological age in both females and males showed a strong positive correlation. {r value=0.987 (females) & 0.974 (males)} and for both p<0.001 which indicates that the correlation of estimated skeletal age and chronological age was statistically significant.Conclusion: Study reveals skeletal age lagged behind chronological age in all age subgroups in both boys and girls but the difference of CA and SA is less in girls as compared to boys. The G-P atlas method, although very old but used with caution can yield good results for assessment of bone age in Indian boys and girls, better for girls. Delay in skeletal maturation was observed in both males and females, but the females in the sample matured earlier then the males. Thus, G-P atlas method seems applicable on Indian children. However, studies on larger sample sizes considering other factors may yield better insight into its forensic application.
背景、目的和目标:年龄估计也是确定个人身份的重要因素之一,放射学检查在结合临床检查估计年龄方面发挥着重要作用。本研究旨在评估 Greulich 和 Pyle(GP)法在斋浦尔地区 14-18 岁健康人群中测定年龄的适用性。研究方法:本研究共对 80 名 14 至 18 岁健康人群进行了测量:本研究在获得斋浦尔 SMS 医学院 RRB 和 IEC 的许可和批准后,于 2020 年 7 月 1 日至 2021 年 8 月 31 日期间在法医学系与放射学系联合对 80 名 14 至 18 岁的受试者进行了研究。所有受试者均来自各年龄分组,即 14-15 岁(A 组)、15-16 岁(B 组)、16-17 岁(C 组)和 17-18 岁(D 组),其中男女受试者人数相等(每个年龄分组各 10 名男性和 10 名女性)。在获得有效的年龄估计知情同意书后,进行所有详细的一般身体和牙齿检查,并记录在建议的表格中。骨骼年龄(骨龄)使用 Greulich 和 Pyle Atlas(1959 年第二版)15 确定。根据性别将计算机上的 X 光图像与 GP 图谱上的图像进行比较,以评估骨骼年龄。根据性别将图像分成两组,由两位观察者在不同场合进行检查,以确定受试者的骨骼年龄并得出结果:在所有受试者(人数=80)中,印度教徒占 81.2%(男性 34 人,女性 31 人),穆斯林教徒占 12.5%(男性 4 人,女性 6 人),耆那教徒占 6.25%(男性 2 人,女性 3 人)。用 GP 法估计的女性和男性骨骼年龄与纪年年龄的相关性显示出很强的正相关性。{r值=0.987(女性)和0.974(男性)},两者的p<0.001,表明估计骨骼年龄与年代年龄的相关性在统计学上有显著意义:研究显示,在所有年龄分组中,男孩和女孩的骨骼年龄都落后于计时年龄,但女孩的CA和SA与男孩相比差异较小。G-P 图集法虽然历史悠久,但在谨慎使用的情况下也能为印度男孩和女孩的骨龄评估提供良好的结果,对女孩的效果更好。男性和女性的骨骼成熟都有延迟,但样本中的女性比男性成熟得早。因此,G-P 图集法似乎适用于印度儿童。不过,考虑到其他因素,对更大样本量的研究可能会对其在法医方面的应用产生更好的影响。
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引用次数: 0
Clinical Profile and Outcome of Drug Abuse Patients Admitted in Intensive Care Unit of a Tertiary Care Hospital in Kashmir: A Prospective Observational Study 克什米尔一家三级医院重症监护室收治的药物滥用患者的临床概况和预后:前瞻性观察研究
Pub Date : 2024-01-18 DOI: 10.37506/08dabt36
Reyaz Ahmed Qadri, S. Sidiq, Majid Jehangir, A. H. Mir, Talib Khan, Abdul Waheed Mir, Majid Khan, MD Senior Resident Mbbs
Introduction: Drug abuse is a global public health issue that poses significant challenges to healthcare systems particularly in intensive care units (ICU). This study aims to assess the clinical profile and outcomes of drug abuse patients admitted to ICU of a tertiary care hospital in North India.Methods: A prospective observational study was conducted over period of 3 years involving drug abuse patients admitted to the ICU. Demographic data, clinical characteristics, substance abuse patterns, co-morbidities, severity scores and outcomes were recorded.Results: A total of 35 patients were included in the study. All patients were males with a mean age of 29.1(± 11.68).25 patients were from rural areas. 42.8% were unemployed. Most of the patients (85.7%) were unmarried. 34.3% were students. Mean APACHE II score on ICU admission was 13. Most of patients were admitted for heroin overdose (60%). All 35 patients admitted in ICU had low Glasgow coma scale (GCS< 7). 32 (91.4%) patients had associated respiratory failure. 7 patients had cardiovascular instability. 4 (11.4%) patients developed multi-organ failure during the ICU stay. Mean duration of ICU stay was 3.4 (± 2.68). 23 (65.7%) patients got extubated within 24 hours of ICU admission. 4 patients expired (11.4% mortality) in study group. Sepsis was cause of death in one patient, 3 patients died of multi-organ failureConclusion: Most of the overdose was due to opioids specifically of heroin. CNS depression was found in all drug overdose patients followed by respiratory failure. Multi organ involvement have poor outcome. Mortality ensued in 11.4% of admitted patients as a result of sepsis and multi-organ failure. Majority of the patients had good outcome especially patients who presented early to healthcare facility.
导言:药物滥用是一个全球性的公共卫生问题,给医疗保健系统,尤其是重症监护病房(ICU)带来了巨大挑战。本研究旨在评估印度北部一家三级医院重症监护室收治的药物滥用患者的临床概况和治疗效果:这项前瞻性观察研究为期 3 年,涉及重症监护室收治的药物滥用患者。研究记录了人口统计学数据、临床特征、药物滥用模式、并发症、严重程度评分和结果:研究共纳入 35 名患者。所有患者均为男性,平均年龄为 29.1(± 11.68)岁。42.8%的患者为失业者。大多数患者(85.7%)未婚。34.3%为学生。入住重症监护室时的平均 APACHE II 评分为 13 分。大多数患者因吸食海洛因过量入院(60%)。重症监护室收治的所有 35 名患者的格拉斯哥昏迷量表(GCS< 7)均较低。32 名患者(91.4%)伴有呼吸衰竭。7 名患者心血管不稳定。4名(11.4%)患者在重症监护室住院期间出现多器官功能衰竭。入住重症监护室的平均时间为 3.4 (± 2.68)。23(65.7%)名患者在入住重症监护室 24 小时内拔管。研究组中有 4 名患者死亡(死亡率为 11.4%)。一名患者死于败血症,3 名患者死于多器官功能衰竭:结论:大多数用药过量都是由于阿片类药物,尤其是海洛因。所有用药过量的患者都出现了中枢神经系统抑制,其次是呼吸衰竭。多器官受累的结果很差。11.4%的入院患者因败血症和多器官功能衰竭而死亡。大多数患者的预后良好,尤其是那些早期到医疗机构就诊的患者。
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引用次数: 0
Integrating Temperature Data with Other Forensic Methods for Time Since Death Estimation 将温度数据与其他法医方法相结合以估算死亡时间
Pub Date : 2024-01-18 DOI: 10.37506/e5hae568
Pradeep kumar Yadav, Rajiv Ratan Singh, Sachin Kumar Tripathi
To reliably calculate the time since death is most important for investigating officers in all unnatural deathcases. Reliability of the study can be obtained only when potential influencing factors are considered during the calculation of time since death. One of the methods used for calculating the time since death is based on the cooling of the body. Cooling of the body is influenced by various internal as well as external factors. There are various body temperature-based methods in practice (Henssge’s rectal nomogram, Henssge’s brain nomogram, and Baccino’s both interval and global formulae based on ear temperature) to estimate the post-mortem interval (PMI). The rectum has been traditionally used to determine the central core temperature after death, though the external auditory canal has been proposed as an alternative site. According to published research, techniques based on ear temperature are just as trustworthy as those based on rectal temperature for determining the early PMI and may be employed as rapid, easy, and non-invasive procedures on the scene. It is vital to keep in mind that other aspects such as rigor mortis, lividity, and decomposition must also be taken into account to achieve a more accurate estimate, even if calculating the time since death based on the cooling of the corpse might be informative.Aim: The goal of this study is to thoroughly examine some of the available approaches, compare the accuracy of the results, and determine which method is more accurate (reliable) at estimating the time of death.Methods: It was decided to evaluate some of the earlier research’ published works from different publications and databases. A digital database was searched. Picks were made at random from the studies that were thought to be pertinent to the present goal.Result: Therefore, in addition to the body’s cooling rate, other factors like post-mortem lividity, rigor mortis,chemical changes in the body, and mechanical and electrical excitability of the skeletal muscles, are crucial for a more accurate prediction of the time of death.Conclusion: It is usually advisable to take into account additional factors in addition to the algor mortis-based one when determining the time since death so that a more exact and trustworthy time of death can assist the investigating officer in more precisely resolving medico-legal matters.
对所有非正常死亡案件的调查人员来说,可靠地计算死亡时间是最重要的。只有在计算死亡时间时考虑到潜在的影响因素,才能获得可靠的研究结果。计算死亡时间的方法之一是以尸体冷却为基础。身体冷却受到各种内部和外部因素的影响。目前有多种基于体温的方法(Henssge 的直肠名义图、Henssge 的脑名义图和 Baccino 基于耳温的间隔和整体公式)来估算死后间隔时间。传统上使用直肠来确定死后的中心核心温度,但也有人提出外耳道作为替代部位。根据已发表的研究,基于耳温的技术与基于直肠温度的技术在确定早期死亡间隔方面同样可信,并且可以在现场作为快速、简便和无创的程序使用。必须牢记的是,为了获得更准确的估计,还必须考虑到其他方面的因素,如尸僵、尸斑和腐烂,即使根据尸体冷却情况计算死亡时间可能具有参考价值。目的:本研究的目的是彻底检查一些可用的方法,比较结果的准确性,并确定哪种方法在估计死亡时间方面更准确(可靠):我们决定从不同的出版物和数据库中评估一些早期研究发表的作品。我们搜索了一个数字数据库。从被认为与当前目标相关的研究中随机挑选:因此,除了身体的冷却速度外,其他因素如死尸的青紫、尸僵、体内的化学变化、骨骼肌的机械和电兴奋性等,对于更准确地预测死亡时间至关重要:结论:通常情况下,在确定死亡时间时,除了基于尸僵算法的因素外,最好还考虑其他因素,以便更准确、更可信的死亡时间可以帮助调查人员更准确地解决医学法律问题。
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引用次数: 0
Evaluation of the Effectiveness of an Alternate Diatom Extraction Procedure in Diatomological Study 硅藻研究中替代硅藻提取程序的效果评估
Pub Date : 2024-01-18 DOI: 10.37506/p474y888
Surangama Chakraborty
Background: The “Diatom Test” is based on the recovery of diatoms in high concentration in the organs of drowned persons because diatoms pass through the alveoli into the great circulation. Acid digested extracts of various internal organs and water collected from waterbodies demonstrate the presence of diatoms. If quantitatively and qualitatively these diatoms match to each other, that is the confirmatory diagnosis of death due to drowning in that drowning medium. This study was conducted to analyze the effectiveness of alternate method of diatom extraction using diluted Sulphuric acid solution and to compare its effectiveness with the traditional method of extraction of diatoms using concentrated nitric acid.Methods: It was a prospective study. Samples were collected from ten different water bodies which included lakes,rivers, canals and reservoirs, for a period of one year in all the seasons. The pH and temperature of water were recorded during sampling. From each of water samples, diatoms were extracted using both classical and alternate method. And then compared by observing the diatoms at 100X magnification under compound microscope. Total 22 genera of diatoms were identified. Most of them were pennales and few were centrales. The time demanded for complete digestion of samples with strong acids like concentrated nitric acid was much lesser time than with diluted acids as it hasstronger digestive capability in comparison to diluted sulphuric acid. The structures of different diatoms specifically Cyclotella, Cybella, Navicula were better found in almost intact state with clear background after digestion by diluted sulphuric acid.Conclusion: Depending on different criterion, digestion by diluted sulphuric acid has procured better result than concentrated nitric acid digestion method. Therefore, for qualitative and quantitative diatom analysis alternate diatom extraction method is a better choice than traditional method.
背景:硅藻测试 "的依据是在溺水者的器官中发现高浓度的硅藻,因为硅藻会通过肺泡进入大循环。从各种内脏器官和水体中采集的酸性消化提取物可证明硅藻的存在。如果这些硅藻在数量和质量上相互吻合,就可以确诊在该溺水介质中因溺水死亡。本研究旨在分析使用稀硫酸溶液提取硅藻的替代方法的有效性,并将其与使用浓硝酸提取硅藻的传统方法进行比较:这是一项前瞻性研究。样本采集自 10 个不同的水体,包括湖泊、河流、运河和水库。取样时记录了水的 pH 值和温度。采用传统方法和交替方法从每个水样中提取硅藻。然后在复合显微镜下用 100 倍放大镜观察硅藻,进行比较。共鉴定出 22 个硅藻属。其中大部分为长颈硅藻属,少数为中心硅藻属。与稀硫酸相比,浓硝酸的消化能力更强,因此用浓硝酸等强酸完全消化样本所需的时间要比稀酸少得多。用稀硫酸消解后,不同硅藻的结构,特别是旋毛藻、水螅藻、脐带藻的结构几乎完好无损,背景清晰:根据不同的标准,稀硫酸消解法比浓硝酸消解法效果更好。因此,对于硅藻的定性和定量分析,替代硅藻提取法是比传统方法更好的选择。
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引用次数: 0
Electrocution Related Deaths in Catchment Area of East Point Hospital: A 3-Year Retrospective Study 东角医院集水区与触电有关的死亡:三年回顾性研究
Pub Date : 2024-01-18 DOI: 10.37506/8n1x6077
Manjunatha K, Raghavendra R, Ravindra Kumar C N
The present retrospective study has been carried out to find the occurrence of deaths due to electrocution inEastern part of Bengaluru with special emphasis on finding out the conditions along with pattern of injuriessustained by the victims. Medico legal autopsy cases with history of electrocution over period of three yearsbetween January 2019 to December 2021 were included in this study. A total of 799 cases were autopsied at our Institute during that period, out of which 51 deaths (6.38%) were deaths due to electrocution, in which male’s outnumbered females. The highest number of cases were observed in the age group of 21-30 years (n=18, 35.3%).27 victims (52.9%) were electrocuted by low tensions domestic supply. Majority of the victims (n=31, 60.8%) died on the spot. Most cases (n=38, 74.5%) were reported during the daytime between 7 am and 7 pm. The incidence of deaths during rainy season were typically higher (n=29, 56.9%). Limbs were the most common body parts affected by electrocution (n=27, 52.9%). 37 (72.5%) victims were electrocuted in dry conditions according to the surrounding places of incidence. As per police documents and history by relatives all cases of the electrocution deaths in the present series were accidental in nature.The hazard of getting electrocuted in domestic surroundings from the chaotically connected electric wires without correct maintenance is certainly a matter of worry. Implementation of correct insulation safety procedures are important factors required for prevention of fatal electrocution.
本回顾性研究旨在了解班加罗尔东部地区发生的触电死亡事件,重点是了解受害者的受伤情况和模式。本研究纳入了 2019 年 1 月至 2021 年 12 月三年期间有触电史的医学法律尸检病例。在此期间,本研究所共对 799 例病例进行了尸检,其中 51 例(6.38%)死于触电,其中男性多于女性。21-30岁年龄组的病例数最多(18例,占35.3%)。27名受害者(52.9%)是因低压家用电源触电死亡。大多数受害者(31 人,占 60.8%)当场死亡。大多数病例(38 人,占 74.5%)发生在上午 7 时至下午 7 时的白天。雨季的死亡发生率通常更高(n=29,56.9%)。四肢是最常见的触电部位(27 人,占 52.9%)。根据周围的事发地点,37 名受害者(72.5%)是在干燥环境下触电的。根据警方文件和亲属提供的病史,本系列中的所有触电死亡案例均为意外事故。实施正确的绝缘安全程序是预防致命触电事故的重要因素。
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引用次数: 0
Fasciotomy in Snake Bite Victims and Renal Outcome in a Tertiary Care Hospital 一家三甲医院为蛇咬伤患者实施的筋膜切开术和肾功能结果
Pub Date : 2024-01-18 DOI: 10.37506/rhb9py71
Sruthi Balla, M. Seenivasan, C. Anandi, S.A.K. Noor Mohammed, P. Nagrajan
Background: Snake bite is a neglected tropical disease. More than 50% of death due to snake bite in the world occurs in India. Acute kidney injury (AKI) is the cause of morbidity and mortality in viper bite. The compartment syndrome is a rare manifestation of snake bite. There is paucity of data on the role of fasciotomy in viper bite.Methods: A prospective observational study was done in patients admitted to a tertiary care hospital in southIndia, with snake bite-associated acute kidney injury, from March 2018 to March 2020.Results: Sixteen % of patients underwent fasciotomy following snake bite. We compared the renal outcome of 21 patients, who had undergone fasciotomy following snake bite, with 65 patients with dialysis-requiring acute kidney injury following snake bite. The patients in the fasciotomy group had a lower peak serum creatinine (5.3 mg/dl), needed less number of dialysis sessions (3.9) and were on lesser number of days (6.1) on dialysis following snake bite, when compared with the group not needing fasciotomy.Conclusion: In snake bite patients presenting with compartment syndrome, fasciotomy should be considered in view of favourable renal outcome.
背景:蛇咬伤是一种被忽视的热带疾病:蛇咬伤是一种被忽视的热带疾病。全世界因被蛇咬伤而死亡的人中,50%以上发生在印度。急性肾损伤(AKI)是毒蛇咬伤的发病和死亡原因。储室综合征是蛇咬伤的一种罕见表现。有关筋膜切开术在毒蛇咬伤中的作用的数据很少:方法:2018 年 3 月至 2020 年 3 月,对印度南部一家三级医院收治的蛇咬伤相关急性肾损伤患者进行了前瞻性观察研究:16%的患者在被蛇咬伤后接受了筋膜切开术。我们比较了 21 名被蛇咬伤后接受筋膜切开术的患者与 65 名被蛇咬伤后需要透析治疗的急性肾损伤患者的肾脏预后。与无需进行筋膜切开术的患者相比,筋膜切开术组患者的血清肌酐峰值(5.3 mg/dl)较低,需要的透析次数(3.9 次)较少,被蛇咬伤后透析的天数(6.1 天)也较少:结论:对于出现储室综合征的蛇咬伤患者,应考虑进行筋膜切开术,以获得良好的肾脏治疗效果。
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引用次数: 0
期刊
Indian Journal of Forensic Medicine &amp; Toxicology
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