Pub Date : 2019-06-01DOI: 10.17582/JOURNAL.PUJZ/2019.34.1.97.100
F. Bilal, Abdulmohsen Alhejaily, S. Husnain
{"title":"Mutational Analysis of PIK3CA Gene from FFPE of Non-Small Cell Lung Cancer in Non-Smokers","authors":"F. Bilal, Abdulmohsen Alhejaily, S. Husnain","doi":"10.17582/JOURNAL.PUJZ/2019.34.1.97.100","DOIUrl":"https://doi.org/10.17582/JOURNAL.PUJZ/2019.34.1.97.100","url":null,"abstract":"","PeriodicalId":52166,"journal":{"name":"Punjab University Journal of Zoology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43982766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-06-01DOI: 10.17582/JOURNAL.PUJZ/2019.34.1.85.88
H. Naz, S. Abdullah, K. Abbas, M. Tariq, Laiba Shafique, G. Nazeer
| In an acute toxicity trail, LC50 and LC100value (96 hr) of endosulfan (END)+chlorpyrifos (CPF) mixture for Catla catla was computed. The genotoxicity of END+CPF mixture in RBCs of fish was also evaluated by comet assay. To check the genotoxicity of END+CPF mixture, blood of fish was sampled after 24-hr of intervals. Some fishes were also kept in clean water known as negative control (NC) and for positive control (PC) cyclophosphamide was injected into fish. The tolerance limits of C. catla against END+CPF was computed as 1.35±0.01μgL(LC50) and 2.25±0.02μgL(LC100). Genotoxic results showed that END+CPF mixture caused significant damage to nuclei (22.58±5.37%) and GDI (0.73±0.15%) in RBCs of fish as compare to control. Aduration-specific damage to DNA was observed in RBCs of C. catla. Article History Received: September 04, 2018 Revised: June 03, 2019 Accepted: June 08, 2019 Published: June 21, 2019 Authors’ Contributions HN performed the research and wrote the article. SA planned and supervised the study. KA provided lab facilities for research. MAT and LS helped in writing the article. GN helped in statistical anlyses.
{"title":"Comet Assay: Quantification of Damaged DNA in Catla catla Exposed to Endosulfan+Chlorpyrifos","authors":"H. Naz, S. Abdullah, K. Abbas, M. Tariq, Laiba Shafique, G. Nazeer","doi":"10.17582/JOURNAL.PUJZ/2019.34.1.85.88","DOIUrl":"https://doi.org/10.17582/JOURNAL.PUJZ/2019.34.1.85.88","url":null,"abstract":"| In an acute toxicity trail, LC50 and LC100value (96 hr) of endosulfan (END)+chlorpyrifos (CPF) mixture for Catla catla was computed. The genotoxicity of END+CPF mixture in RBCs of fish was also evaluated by comet assay. To check the genotoxicity of END+CPF mixture, blood of fish was sampled after 24-hr of intervals. Some fishes were also kept in clean water known as negative control (NC) and for positive control (PC) cyclophosphamide was injected into fish. The tolerance limits of C. catla against END+CPF was computed as 1.35±0.01μgL(LC50) and 2.25±0.02μgL(LC100). Genotoxic results showed that END+CPF mixture caused significant damage to nuclei (22.58±5.37%) and GDI (0.73±0.15%) in RBCs of fish as compare to control. Aduration-specific damage to DNA was observed in RBCs of C. catla. Article History Received: September 04, 2018 Revised: June 03, 2019 Accepted: June 08, 2019 Published: June 21, 2019 Authors’ Contributions HN performed the research and wrote the article. SA planned and supervised the study. KA provided lab facilities for research. MAT and LS helped in writing the article. GN helped in statistical anlyses.","PeriodicalId":52166,"journal":{"name":"Punjab University Journal of Zoology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47323543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-06-01DOI: 10.17582/JOURNAL.PUJZ/2019.34.1.61.71
Ghalia Nawal, M. Irfan, Saira Ashfaq, H. A. Shakir
{"title":"An Overview of Bacterial Lipases and their Enormous Applications","authors":"Ghalia Nawal, M. Irfan, Saira Ashfaq, H. A. Shakir","doi":"10.17582/JOURNAL.PUJZ/2019.34.1.61.71","DOIUrl":"https://doi.org/10.17582/JOURNAL.PUJZ/2019.34.1.61.71","url":null,"abstract":"","PeriodicalId":52166,"journal":{"name":"Punjab University Journal of Zoology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43517007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-06-01DOI: 10.17582/JOURNAL.PUJZ/2019.34.1.73.77
M. A. Chowdhury, M. Karim, Md Rahman, S. H. T. Shefat, Ashikur Rahman, M. A. Hossain
{"title":"Biodiversity Assessment of Indigenous Fish Species in the Surma River of Sylhet Sadar, Bangladesh","authors":"M. A. Chowdhury, M. Karim, Md Rahman, S. H. T. Shefat, Ashikur Rahman, M. A. Hossain","doi":"10.17582/JOURNAL.PUJZ/2019.34.1.73.77","DOIUrl":"https://doi.org/10.17582/JOURNAL.PUJZ/2019.34.1.73.77","url":null,"abstract":"","PeriodicalId":52166,"journal":{"name":"Punjab University Journal of Zoology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42872934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-06-01DOI: 10.17582/JOURNAL.PUJZ/2019.34.1.89.95
Muhammad Naveed Sheas, Haris Rasool, Muhammad Nouman Rafique, M. Tariq, A. Muhammad, Khubaib Ali
| Depression is a common psychiatric disorder which is characterized by mood swings, aversion from activity and apathy which affects the thoughts, behavior and feelings of wellbeing. Honey and curcumin, traditional folk medicines, are thought to steadfast the defense mechanisms of body against oxidative stress which is a key factor in elevating depression. This study is designed to reveal the efficacy of honey and curcumin against depression in rat model. 24 male wister rats were randomly divided into 4 groups. After the trial, blood biochemistry was done in which antioxidant enzymes like SOD, Catalyze, Glutathione peroxidase were performed that shows significantly high levels in the blood than the group that was treated with nicotine and shows a positive correlation with the days at which the rat’s groups were given a supplemented feed. Total antioxidant capacity and antioxidative stress shows significant results by the selected treatment. Article History Received: September 24, 2018 Revised: March 10, 2019 Accepted: June 03, 2019 Published: June 26, 2019 Authors’ Contributions MNS identified the research area, prepared and conducted the research. HR and KA assisted in provision of research material. MNR helped in experiments and writing the manuscript. MRT assisten in designing the project. AM helped in sampling.
{"title":"Exploring the Potential of Honey and Curcumin as Antidepressent","authors":"Muhammad Naveed Sheas, Haris Rasool, Muhammad Nouman Rafique, M. Tariq, A. Muhammad, Khubaib Ali","doi":"10.17582/JOURNAL.PUJZ/2019.34.1.89.95","DOIUrl":"https://doi.org/10.17582/JOURNAL.PUJZ/2019.34.1.89.95","url":null,"abstract":"| Depression is a common psychiatric disorder which is characterized by mood swings, aversion from activity and apathy which affects the thoughts, behavior and feelings of wellbeing. Honey and curcumin, traditional folk medicines, are thought to steadfast the defense mechanisms of body against oxidative stress which is a key factor in elevating depression. This study is designed to reveal the efficacy of honey and curcumin against depression in rat model. 24 male wister rats were randomly divided into 4 groups. After the trial, blood biochemistry was done in which antioxidant enzymes like SOD, Catalyze, Glutathione peroxidase were performed that shows significantly high levels in the blood than the group that was treated with nicotine and shows a positive correlation with the days at which the rat’s groups were given a supplemented feed. Total antioxidant capacity and antioxidative stress shows significant results by the selected treatment. Article History Received: September 24, 2018 Revised: March 10, 2019 Accepted: June 03, 2019 Published: June 26, 2019 Authors’ Contributions MNS identified the research area, prepared and conducted the research. HR and KA assisted in provision of research material. MNR helped in experiments and writing the manuscript. MRT assisten in designing the project. AM helped in sampling.","PeriodicalId":52166,"journal":{"name":"Punjab University Journal of Zoology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42234463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-06-01DOI: 10.17582/JOURNAL.PUJZ/2019.34.1.79.84
F. Tufail, S. Abdullah, H. Naz, K. Abbas, Laiba Shafique
| From last few years contamination of aquatic bodies has been increased due to extensive use of pesticides in agriculture. The exposure of aquatic animals to these pesticides results in acute and chronic health effects. Fresh water fish species such as Channa striata serve as important bio-indicators for aquatic contamination to access the changes caused by human activities effectively and reliable monitoring bio-system to recognize and predict hazardous effects of pollutants. Therefore, this study was done to evaluate the toxicity of endosulfan (END), deltamethrin (DM) mixture on hepatic catalase (CAT) activity of fish, Channa striata exposed to the sub-lethal concentration (1/3rd of LC50) for 14 days. The CAT was partially purified by using ammonium sulphate precipitation technique. Results of partial purification demonstrated that the exposure of insecticides mixture decreased the hepatic CAT activity in fish (43.33±1.41 UmL-1) as compare to control (50.33±1.41 Unit mL-1). After desalting specific activity of hepatic CAT remain lowest in exposed fish as 134.30±0.71 Unit mg-1in relation to control (148.02±0.41 Umg-1). The fold purification of hepatic CAT from control and exposed C. striata was noted as 2.35±0.01 and 2.22±0.01, respectively. The percentage recovery of hepatic CAT from control and exposed C. striata was calculated as 81.77±1.41and 69.94±0.71, respectively. Results of kinetic characterization showed that optimum pH and temperature for hepatic CAT was noted as 7.5 and 25oC, respectively. It was concluded that the insecticides mixture exposure can alter the activity of antioxidant enzymes in fish. T hese parameters can be used as a good biomarker to evaluate the aquatic pollution. Article History Received: September 08, 2018 Revised: March 12, 2019 Accepted: May 05, 2019 Published: June 21, 2019 Authors’ Contributions FT performed the research and wrote the article. SA planned and supervised the study. HN helped in statistical anlyses. KA provided lab facilities for research. LS helped in writing the article.
{"title":"Purification and Kinetic Characterization of Hepatic Catalase from Carnivore Fish, Channa striata Exposed to Agrochemicals (endosulfan+deltamethrin)","authors":"F. Tufail, S. Abdullah, H. Naz, K. Abbas, Laiba Shafique","doi":"10.17582/JOURNAL.PUJZ/2019.34.1.79.84","DOIUrl":"https://doi.org/10.17582/JOURNAL.PUJZ/2019.34.1.79.84","url":null,"abstract":"| From last few years contamination of aquatic bodies has been increased due to extensive use of pesticides in agriculture. The exposure of aquatic animals to these pesticides results in acute and chronic health effects. Fresh water fish species such as Channa striata serve as important bio-indicators for aquatic contamination to access the changes caused by human activities effectively and reliable monitoring bio-system to recognize and predict hazardous effects of pollutants. Therefore, this study was done to evaluate the toxicity of endosulfan (END), deltamethrin (DM) mixture on hepatic catalase (CAT) activity of fish, Channa striata exposed to the sub-lethal concentration (1/3rd of LC50) for 14 days. The CAT was partially purified by using ammonium sulphate precipitation technique. Results of partial purification demonstrated that the exposure of insecticides mixture decreased the hepatic CAT activity in fish (43.33±1.41 UmL-1) as compare to control (50.33±1.41 Unit mL-1). After desalting specific activity of hepatic CAT remain lowest in exposed fish as 134.30±0.71 Unit mg-1in relation to control (148.02±0.41 Umg-1). The fold purification of hepatic CAT from control and exposed C. striata was noted as 2.35±0.01 and 2.22±0.01, respectively. The percentage recovery of hepatic CAT from control and exposed C. striata was calculated as 81.77±1.41and 69.94±0.71, respectively. Results of kinetic characterization showed that optimum pH and temperature for hepatic CAT was noted as 7.5 and 25oC, respectively. It was concluded that the insecticides mixture exposure can alter the activity of antioxidant enzymes in fish. T hese parameters can be used as a good biomarker to evaluate the aquatic pollution. Article History Received: September 08, 2018 Revised: March 12, 2019 Accepted: May 05, 2019 Published: June 21, 2019 Authors’ Contributions FT performed the research and wrote the article. SA planned and supervised the study. HN helped in statistical anlyses. KA provided lab facilities for research. LS helped in writing the article.","PeriodicalId":52166,"journal":{"name":"Punjab University Journal of Zoology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42660519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-06-01DOI: 10.17582/JOURNAL.PUJZ/2019.34.1.101.104
Sehrish Ashraaf, H. M. Tahir, M. Qazi, Shaukat Ali
{"title":"Variations in Nucleotide Frequency Distribution in Spider Families","authors":"Sehrish Ashraaf, H. M. Tahir, M. Qazi, Shaukat Ali","doi":"10.17582/JOURNAL.PUJZ/2019.34.1.101.104","DOIUrl":"https://doi.org/10.17582/JOURNAL.PUJZ/2019.34.1.101.104","url":null,"abstract":"","PeriodicalId":52166,"journal":{"name":"Punjab University Journal of Zoology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41288394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-06-01DOI: 10.17582/JOURNAL.PUJZ/2019.34.1.51.53
G. Murtaza, R. Sultana, M. A. Malik, Sibtain Afzal, Mubashar Hussain, S. Bashir
| Nicotine smoking is one of the leading causes of preventable diseases and premature death worldwide. Nicotine is a highly addictive substance, which exerts influence over different reward pathways of the brain. In Pakistan, nicotine use is very high and its consumption is expected to increase further in future. This article focuses on the increase of nicotine consumption in Pakistan and how people can get rid of smoking habit using the latest method such as transcranial direct current stimulation. Article History Received: March 03, 2018 Revised: April 17, 2019 Accepted: May 20, 2019 Published: June 11, 2019 Authors’ Contributions GM wrote the manuscript with input from all the authors. RS, SA and MAM analysed the data. MH and SB revised the manuscript.
{"title":"Transcranial Direct Current Stimulation: An Alternative Approach to Quit Smoking","authors":"G. Murtaza, R. Sultana, M. A. Malik, Sibtain Afzal, Mubashar Hussain, S. Bashir","doi":"10.17582/JOURNAL.PUJZ/2019.34.1.51.53","DOIUrl":"https://doi.org/10.17582/JOURNAL.PUJZ/2019.34.1.51.53","url":null,"abstract":"| Nicotine smoking is one of the leading causes of preventable diseases and premature death worldwide. Nicotine is a highly addictive substance, which exerts influence over different reward pathways of the brain. In Pakistan, nicotine use is very high and its consumption is expected to increase further in future. This article focuses on the increase of nicotine consumption in Pakistan and how people can get rid of smoking habit using the latest method such as transcranial direct current stimulation. Article History Received: March 03, 2018 Revised: April 17, 2019 Accepted: May 20, 2019 Published: June 11, 2019 Authors’ Contributions GM wrote the manuscript with input from all the authors. RS, SA and MAM analysed the data. MH and SB revised the manuscript.","PeriodicalId":52166,"journal":{"name":"Punjab University Journal of Zoology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48225051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.17582/journal.pujz/2019.34.1.105.113
J. Odigie
{"title":"Application of Water Quality and Pollution Tolerance Indexes as Effective Tools for River Management","authors":"J. Odigie","doi":"10.17582/journal.pujz/2019.34.1.105.113","DOIUrl":"https://doi.org/10.17582/journal.pujz/2019.34.1.105.113","url":null,"abstract":"","PeriodicalId":52166,"journal":{"name":"Punjab University Journal of Zoology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67576424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.17582/journal.pujz/2019.34.1.127.132
Maliha Ayub, M. Javed, Fariha Latif, F. Ambreen, K. Sabir
{"title":"Tissue Specific Changes in the Antioxidant Enzyme Activity of Manganese Exposed Labeo rohita","authors":"Maliha Ayub, M. Javed, Fariha Latif, F. Ambreen, K. Sabir","doi":"10.17582/journal.pujz/2019.34.1.127.132","DOIUrl":"https://doi.org/10.17582/journal.pujz/2019.34.1.127.132","url":null,"abstract":"","PeriodicalId":52166,"journal":{"name":"Punjab University Journal of Zoology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67576800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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