Matrix Biology Plus最新文献

{"title":"Revealing sex-specific changes across protein structure in the aging bone extracellular matrix","authors":"Jacob Tudor ,&nbsp;Alexander Eckersley ,&nbsp;Michael Buckley","doi":"10.1016/j.mbplus.2025.100189","DOIUrl":"10.1016/j.mbplus.2025.100189","url":null,"abstract":"<div><div>The process of aging is an integral but complex component of life that has been intensely studied for decades, from the molecular level to whole organisms. At the tissue level, bone is one of the most difficult to study due to its composite nature of inorganic and organic phases, but advancements in proteomics are enhancing our understanding of the latter, improving our understanding of skeletal aging through identifying temporal changes across the bone proteome. The relative longevity of extracellular matrix (ECM) proteins can make them more susceptible to accumulating damage modifications over time. In addition, their informational density, including their 2D and 3D structure, protein folding, post translational modifications and proteomic composition, as well as their functional importance, has made them a target of interest in the study of aging and medical conditions such as osteoporosis and arthritis. ECM proteins are also increasingly utilised in forensic science for determining biological sex/age because of their longevity. Following recent developments in peptide location fingerprinting methods that improve capabilities of identifying regional changes in protein structure, this study aimed to identify age-associated regional changes along protein structures in <em>Rattus norvegicus</em> from whole limb LC-MC/MS data. Regional changes in protein structure were identified in a variety of collagenous and non-collagenous ECM proteins, providing evidence for increased remodeling in juvenile rats and a reduced ability in adult rats, alongside damage accumulation in the adult ECM. This research highlights the importance of fibrillar collagen remodeling but is also indicative of potential new roles for osteopontin, thrombin, apolipoproteins and wider ECM regulators such as cartilage oligomeric matrix protein. This demonstrates, in this case, the utility of peptide location fingerprinting as a screening tool to identify biomarker candidates of bone aging between juvenile and adult rats.</div></div>","PeriodicalId":52317,"journal":{"name":"Matrix Biology Plus","volume":"29 ","pages":"Article 100189"},"PeriodicalIF":0.0,"publicationDate":"2025-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145926747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"P-LM421E8, the heparan sulfate chain-conjugated laminin-421-E8 fragment, drives differentiation of human induced pluripotent stem cells into hematopoietic progenitor cells comparable to basic fibroblast growth factor in a chemically defined system","authors":"Naoto Ninomiya ,&nbsp;Kaoru Sasaki ,&nbsp;Ryosuke Katori ,&nbsp;Yasuhiro Shimizu ,&nbsp;Kazumasa Fujita ,&nbsp;Yukimasa Taniguchi ,&nbsp;Taiko Kunieda ,&nbsp;Kouichi Tamura ,&nbsp;Masashi Yamada ,&nbsp;Kiyotoshi Sekiguchi ,&nbsp;Hironobu Kimura","doi":"10.1016/j.mbplus.2025.100188","DOIUrl":"10.1016/j.mbplus.2025.100188","url":null,"abstract":"<div><div>Human induced pluripotent stem cells (hiPSCs) are a promising source for cell-based and regenerative therapies. In this study, we developed and optimized a chemically defined differentiation protocol to generate hematopoietic progenitor cells (HPCs) from hiPSCs. We demonstrated that basic fibroblast growth factor (bFGF) plays a crucial role in enhancing HPC differentiation, particularly when applied during both the mesoderm (ME) and hematopoietic endothelial (HE) induction stages. Additionally, we explored the use of P-LM421E8, a recombinant fusion protein of laminin421-E8 fragment with domain 1 (D1) of perlecan possessing heparan sulfate (HS) chains. Our findings indicate that P-LM421E8 effectively supports HPC differentiation, generating stable CD34-high/CD117⁺ populations comparable to those produced with bFGF treatment. Furthermore, we observed that HPCs generated on P‑LM421E8‑coated surfaces exhibited superior natural killer (NK) cell differentiation potential. Although both P-LM421E8 and bFGF supported HPC differentiation, no significant additive effects were observed when used together. This suggests that P-LM421E8 can effectively support HPC differentiation without the need for exogenous bFGF, possibly through enhanced interaction with endogenous FGFs. The structural properties of P-LM421E8, which facilitate retention and presentation of FGFs via HS chains on its D1, may contribute to its effectiveness in promoting HPC development. Our findings establish P‑LM421E8 as a potent matrix for HPC differentiation and highlight its promise for refining hematopoietic protocols and advancing cell‑based immunotherapies.</div></div>","PeriodicalId":52317,"journal":{"name":"Matrix Biology Plus","volume":"29 ","pages":"Article 100188"},"PeriodicalIF":0.0,"publicationDate":"2025-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145738941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Generation of a conditional Adamts6 mouse allele reveals roles in lung maturation in addition to cardiac and musculoskeletal development","authors":"Jessica M. Sirek ,&nbsp;Elizabeth H. Rush ,&nbsp;Aditi Darodkar ,&nbsp;Suneel S. Apte ,&nbsp;Timothy J. Mead","doi":"10.1016/j.mbplus.2025.100186","DOIUrl":"10.1016/j.mbplus.2025.100186","url":null,"abstract":"<div><div>Prior analysis of mouse embryos homozygous for a point mutation (p.Ser149Arg) that abrogated secretion of the protease, ADAMTS6, showed that it is essential for cardiovascular and limb development. Because <em>Adamts6</em>^S149R/S149R mice do not survive past birth, it is currently not feasible to investigate ADAMTS6 in specific cellular lineages postnatally. Therefore, we generated a conditional allele using CRISPR-Cas9-mediated genome editing to insert unidirectional loxP sites flanking the first coding exon in <em>Adamts6</em>, resulting in a frameshift mutation after loxP recombination. Mice homozygous for the unrecombined floxed allele (<em>Adamts6</em>^fl/fl) are viable, fertile, and without overt phenotype. <em>Adamts6</em>^del/del embryos, generated upon constitutive recombination induced by a <em>CMV</em>-Cre transgene, also do not survive past birth and have identical defects in the heart and limbs as <em>Adamts6</em>^S149R/S149R embryos, demonstrating efficient transgene recombination. In addition to previous defects in cardiovascular and limb development, <em>Adamts6</em>^del/del embryos have reduced airway branching, thereby identifying a role for ADAMTS6 in lung maturation. This newly generated <em>Adamts6</em>^fl allele makes feasible analysis of ADAMTS6 secreted by cells of different lineages and during specified temporal windows during developmental processes as well as in disease models.</div></div>","PeriodicalId":52317,"journal":{"name":"Matrix Biology Plus","volume":"28 ","pages":"Article 100186"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145623743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Tissue material properties, whole-bone morphology and mechanical behavior in the Fbn1C1041G/+ mouse model of Marfan Syndrome” [Matrix Biol. Plus 23 (2024) 100155]","authors":"Elizabeth A. Zimmermann ,&nbsp;Taylor DeVet ,&nbsp;Myriam Cilla ,&nbsp;Laia Albiol ,&nbsp;Kyle Kavaseri ,&nbsp;Christine Andrea ,&nbsp;Catherine Julien ,&nbsp;Kerstin Tiedemann ,&nbsp;Arash Panahifar ,&nbsp;Sima Alidokht ,&nbsp;Richard Chromik ,&nbsp;Svetlana V. Komarova ,&nbsp;Dieter P. Reinhardt ,&nbsp;Paul Zaslansky ,&nbsp;Bettina M. Willie","doi":"10.1016/j.mbplus.2025.100176","DOIUrl":"10.1016/j.mbplus.2025.100176","url":null,"abstract":"","PeriodicalId":52317,"journal":{"name":"Matrix Biology Plus","volume":"28 ","pages":"Article 100176"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145693603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retraction notice to “Deregulated expression of Elastin Microfibril Interfacer 2 (EMILIN2) in gastric cancer affects tumor growth and angiogenesis.” [Matrix Biol. Plus 6–7 (2020) 100029]","authors":"Eva Andreuzzi ,&nbsp;Albina Fejza ,&nbsp;Alessandra Capuano ,&nbsp;Evelina Poletto ,&nbsp;Eliana Pivetta ,&nbsp;Roberto Doliana ,&nbsp;Rosanna Pellicani ,&nbsp;Andrea Favero ,&nbsp;Stefania Maiero ,&nbsp;Mara Fornasarig ,&nbsp;Renato Cannizzaro ,&nbsp;Renato V. Iozzo ,&nbsp;Paola Spessotto ,&nbsp;Maurizio Mongiat","doi":"10.1016/j.mbplus.2025.100184","DOIUrl":"10.1016/j.mbplus.2025.100184","url":null,"abstract":"","PeriodicalId":52317,"journal":{"name":"Matrix Biology Plus","volume":"28 ","pages":"Article 100184"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145693602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Convergence research in matrix biology and biomaterials science","authors":"Daniel L. Alge ,&nbsp;Ashley Brown","doi":"10.1016/j.mbplus.2025.100180","DOIUrl":"10.1016/j.mbplus.2025.100180","url":null,"abstract":"","PeriodicalId":52317,"journal":{"name":"Matrix Biology Plus","volume":"28 ","pages":"Article 100180"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145693601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Presence of type IIB procollagen in mouse articular cartilage and growth plate is revealed by immuno-histochemical analysis with a novel specific antibody” [Matrix Biol. Plus 18 (2023) 100130]","authors":"Emeline Perrier-Groult,&nbsp;Shérine Moustaghfir,&nbsp;Marielle Pasdeloup,&nbsp;Jean-Daniel Malcor,&nbsp;Jérôme Lafont,&nbsp;Frédéric Mallein-Gerin","doi":"10.1016/j.mbplus.2025.100177","DOIUrl":"10.1016/j.mbplus.2025.100177","url":null,"abstract":"","PeriodicalId":52317,"journal":{"name":"Matrix Biology Plus","volume":"28 ","pages":"Article 100177"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145690605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Obesity-driven changes in breast extracellular matrix exhibit a pro-angiogenic phenotype” [Matrix Biol. Plus 24 (2024) 100162]","authors":"Ellen E. Bamberg ,&nbsp;Mark Maslanka ,&nbsp;Kiran Vinod-Paul ,&nbsp;Sharon Sams ,&nbsp;Erica Pollack ,&nbsp;Matthew Conklin ,&nbsp;Peter Kabos ,&nbsp;Kirk C. Hansen","doi":"10.1016/j.mbplus.2025.100178","DOIUrl":"10.1016/j.mbplus.2025.100178","url":null,"abstract":"","PeriodicalId":52317,"journal":{"name":"Matrix Biology Plus","volume":"28 ","pages":"Article 100178"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145693689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Latent-Transforming growth factor − β Binding protein 1 (LTBP1) in corneal stroma","authors":"Isaac Poonen-Honig ,&nbsp;Ana C Acosta ,&nbsp;Mei Sun ,&nbsp;Victoria Emerick ,&nbsp;Marcel Y Avila ,&nbsp;Curtis E. Margo ,&nbsp;Edgar M Espana","doi":"10.1016/j.mbplus.2025.100185","DOIUrl":"10.1016/j.mbplus.2025.100185","url":null,"abstract":"<div><div>We aim to determine the presence and function of LTBP1 in the corneal stroma. We investigated the temporal and spatial corneal expression of LTBP1, and microfibril-associated glycoprotein 1 (MAGP1), known components of the elastic system and regulators of TGF-β storage and latency. Protein quantification −blotting and proteomics-, immunofluorescence microscopy, mRNA analysis and a luciferase assay were used to study the regulation of transforming growth factor β (TGF-β) by LTBP1 in cornea derived fibroblasts and myofibroblasts. Expression of LTBP1 and MAGP1 was found in adult corneas, and absent or minimal in the scleral stroma. Quantitative polymerase chain reaction and protein blotting analyses showed upregulation of these proteins during early postnatal development and deposition in the corneal matrix with tissue maturation. In vitro, LTBP1 regulated the conversion of fibroblasts to myofibroblasts and maintained TGF-β latent in the matrix. This study shows that LTBP1 and MAGP1, regulators of TGF-β activation and elastic fiber components, are found in adult stroma. LTBP1 is a regulator of TGF-β storage and latency and controls conversion of fibroblast to myofibroblast.</div></div>","PeriodicalId":52317,"journal":{"name":"Matrix Biology Plus","volume":"28 ","pages":"Article 100185"},"PeriodicalIF":0.0,"publicationDate":"2025-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145528265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Loss of fibronectin fiber tension is inherent to ECM remodeling in human myocarditis and post-inflammatory fibrosis","authors":"Krishna Chander Sridhar ,&nbsp;Julia Mehl ,&nbsp;Karin Klingel ,&nbsp;Mario Thiele ,&nbsp;Sophie Van Linthout ,&nbsp;Carsten Tschöpe ,&nbsp;Georg N Duda ,&nbsp;Viola Vogel","doi":"10.1016/j.mbplus.2025.100182","DOIUrl":"10.1016/j.mbplus.2025.100182","url":null,"abstract":"<div><h3>Background</h3><div>Viral myocarditis (MC) is associated with extracellular matrix (ECM) remodeling and involves excessive deposition of collagen and other ECM proteins by cardiac fibroblasts, potentially leading to scarring and ECM stiffening, which may subsequently contribute to impaired cardiac functions. Clarifying whether changes in ECM fiber tension are detectable during either the acute or scarring phase could provide a novel, mechanistically relevant mechanobiological signature.</div></div><div><h3>Objectives</h3><div>Our goal was to ask whether ECM mechano-markers can be identified in the myocardium, beyond excessive collagen fiber deposition, that are associated with the acute infection or the pathological scarring of the human heart, and how this might be associated with the infiltration of macrophages.</div></div><div><h3>Methods</h3><div>Left-ventricular (LV) endomyocardial biopsies were obtained from patients (N = 39) with acute myocarditis MC (N = 21) including COVID-19 (N = 4) patients suspected with myocarditis MC and/or impaired LV function, dilated cardiomyopathy (N = 6), inflammatory dilated cardiomyopathy (N = 12) to specify diagnosis and treatment options. Endomyocardial biopsies were analyzed for viral genomes, immune cells infiltration and ECM remodeling. Fibronectin fiber tension was assessed using the fibronectin-binding tension-sensor (FnBPA5), while collagen fiber deposits were visualized using second harmonic generation (SHG) microscopy.</div></div><div><h3>Results</h3><div>While fibronectin fibers were tensed in healthy hearts, histological staining with our novel tension probe FnBPA5 showed that fibronectin fibers had lost their tension in distinct loci in all patient groups. In the acute inflammatory phase (MC), loci with untensed fibronectin fibers were found in close proximity to infiltrated macrophages. In already dilated hearts, biopsies which presented low densities of infiltrated macrophages, thick collagen I/III fiber bundles as visualized by SHG were found in proximity to untensed fibronectin fibers and myofibroblasts, which together are indicative of fibrotic ECM niches where the inflammation has subsided. Comparison of clinical diagnostic and experimental histological data showed clear correlations between altered ECM niche properties and cardiac function deterioration.</div></div><div><h3>Conclusions</h3><div>Our data suggest that relaxed fibronectin fibers are a recurrent feature of myocarditis and associate with measures of cardiac dysfunction. These findings suggest that fibronectin fiber tension might be a mechanobiological signature that warrants validation in larger, longitudinal cohorts and evaluation of in-vivo measurability.</div></div>","PeriodicalId":52317,"journal":{"name":"Matrix Biology Plus","volume":"28 ","pages":"Article 100182"},"PeriodicalIF":0.0,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145229686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}