Journal of Mass Spectrometry and Advances in the Clinical Lab最新文献_第2页

A simple, rapid and cost-effective UHPLC–MS/MS method for simultaneous quantitation of seven antiepileptic drugs/metabolites in human serum 一种简便、快速、经济高效的UHPLC-MS /MS同时定量人血清中七种抗癫痫药物/代谢物的方法

IF 3.4 4区医学 Q2 MEDICAL LABORATORY TECHNOLOGY

Journal of Mass Spectrometry and Advances in the Clinical Lab

Pub Date : 2025-09-13 DOI: 10.1016/j.jmsacl.2025.09.001

Xiaowei Fu, Xiangtang Li, Hui He

Background

Epilepsy affects approximately 50 million people worldwide. Antiepileptic drugs (AEDs) are the mainstream treatment. Therapeutic drug monitoring (TDM) of AEDs is necessary to maximize efficacy and minimize toxicity. We report a simple, rapid, and cost-effective ultra-performance liquid chromatography-mass spectrometry method that can simultaneously measure seven AEDs/metabolites, including levetiracetam (LEV), lacosamide (LCM), zonisamide (ZON), lamotrigine (LMT), 10-hydroxycarbazepine (OXC-M1), clobazam (CLO), and N-desmethyl clobazam (N-CLB) in serum.

Method

Only 20 µl of serum was used with simple protein precipitation and dilution. Analysis was performed on a SCIEX 6500 UHPLC–MS/MS in positive ion mode. Separation was performed on a C18 reversed-phase column using a gradient. The seven AEDs/metabolites were eluted in 4.5 min.

Results

The assay was linear over the concentration ranges 0.4–100 µg/mL for LEV, 0.12–30 µg/mL for LMT, 0.12–30 µg/mL for LCM, 0.32–80 µg/mL for ZON, 0.28–70 µg/mL for OXC-M1, and 7.82–2000 ng/mL for CLO, 78.2–20000 ng/mL for N-CLB, respectively, with correlation coefficient greater than 0.99. Recovery was from 88 to 108 %. Intra and inter assay precision for three levels of quality controls were from 2.1 to 6.8 % and 4.2 to 10.9 %, respectively. The accuracy was evaluated by comparing with the College of American Pathologists survey results, and a correlation coefficient greater than 0.96 was observed. The absence of matrix effects was also confirmed.

Conclusion

We have developed and validated a simple, rapid, and cost-effective UHPLC–MS/MS method for the simultaneous quantitation of seven AEDs/metabolites in serum within a 4.5-min analysis time. It has been implemented in our children’s hospital with same-day turnaround time.

全世界约有5000万人患有癫痫。抗癫痫药物（AEDs）是主流的治疗方法。治疗性药物监测（TDM）是实现抗癫痫药疗效最大化和毒性最小化的必要手段。本文报道了一种简单、快速、经济高效的超高效液相色谱-质谱联用方法，可同时测定血清中7种AEDs/代谢物，包括左乙拉西坦（LEV）、拉科沙胺（LCM）、唑尼沙胺（ZON）、拉莫三嗪（LMT）、10-羟基卡西平（OXC-M1）、氯巴唑（CLO）和n -二甲基氯巴唑（N-CLB）。方法仅用20µl血清进行简单的蛋白沉淀稀释。在SCIEX 6500 UHPLC-MS /MS上进行正离子模式分析。在C18反相柱上使用梯度进行分离。7种aed /代谢物在4.5 min内洗脱。结果LEV、LMT、LCM、ZON、OXC-M1、CLO、N-CLB分别在0.4 ~ 100µg/mL、0.12 ~ 30µg/mL、0.32 ~ 80µg/mL、0.28 ~ 70µg/mL、7.82 ~ 2000 ng/mL、78.2 ~ 20000 ng/mL浓度范围内呈线性关系，相关系数均大于0.99。回收率为88% ~ 108%。三级质量控制的内、间精密度分别为2.1 ~ 6.8%和4.2 ~ 10.9%。与美国病理学家学会调查结果进行比较，相关系数大于0.96。也证实了不存在基质效应。结论建立了一种高效液相色谱-质谱联用（UHPLC-MS /MS）方法，可在4.5 min的分析时间内同时测定血清中7种aed /代谢物。它已在我们的儿童医院实施，当日周转时间。

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引用次数: 0

Corrigendum to “A rapid method for determination of rosuvastatin in blood plasma with supported liquid extraction” [J. Mass Spectromet. Adv. Clin. Lab 36 (2025) 29–36] “支持液体萃取法快速测定血浆中瑞舒伐他汀”的勘误[J]。Spectromet质量。放置中国。实验室36 (2025)29-36]

IF 3.4 4区医学 Q2 MEDICAL LABORATORY TECHNOLOGY

Journal of Mass Spectrometry and Advances in the Clinical Lab

Pub Date : 2025-09-02 DOI: 10.1016/j.jmsacl.2025.08.001

Tjaša Dermota , Mojca Božič Mijovski , Jurij Trontelj

引用次数: 0

Evaluation of the detuning ratio as a tool to detect potential interference in LC-MSMS analysis 失谐比作为LC-MSMS分析中潜在干扰检测工具的评价

IF 3.1 4区医学 Q2 MEDICAL LABORATORY TECHNOLOGY

Journal of Mass Spectrometry and Advances in the Clinical Lab

Pub Date : 2025-07-18 DOI: 10.1016/j.jmsacl.2025.07.002

Arber Rexhaj , Michael Vogeser , Katharina Habler

Objective

Tandem mass spectrometry (MS/MS) is highly specific in principle, but there is always the possibility of interference due to unexpected substances in the samples that have the identical mass transitions as the target analytes (isomeric/isobaric interferences). By recording the ion ratio (IR), clinical laboratories already widely attempt to identify such interferences in individual cases. To supplement this procedure, differential tuning effects can be assessed. We aimed to evaluate this approach experimentally.

Methods

The detuning ratio (DR) is based on the differential influences of MS instrument settings on the ion yield of a respective target analyte; isomeric or isobaric interferences can lead to a shift of the DR for an affected sample. By determining the DR in samples in which known isomeric interference substances have been spiked to the target analyte, the applicability of DR detection was quantitatively investigated.