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Short communications: Effect of red spinach (Amaranthus tricolor L. var Blitumbrum) extract in the diet on color brightnes of guppy fish (Poecilia reticulata) 简短交流:饲料中添加红菠菜(Amaranthus tricolor L. var Blitumbrum)提取物对孔雀鱼色度的影响
Pub Date : 2023-02-27 DOI: 10.13170/ajas.8.1.26813
E. Yusni, Nurul F.D. Hasibuan
Fish color brightness is influenced by food intake, but no prior reviews have explored the use of red spinach extract in fish feed to enhance color brightness and promote growth in guppy (Poecilia reticulata). Therefore, this study aims to determine the suitable concentration of red spinach extract Amaranthus tricolor color brightness, growth, and survival of guppy. The experiment followed a completely randomized design with three concentrations of red spinach extract (3%, 4%, and 5%) replicated three times at the UPTD Fish Breeding Center in North Binjai Regency, North Sumatra Province. Furthermore, red spinach extract was mixed with the commercial diet containing 41% crude protein and administered to fish at a rate of 3% of their body weight daily for 40 days. The results showed a significant effect of red spinach extract doses on guppy color brightness (P0.05), but no significant influence on weight gain and fish survival (P0.05). It was also discovered that the average color increase was 2,652 in feed mixed with 5% red spinach extract. Similarly, the highest fish weight gain was observed at the 5% concentration, which did not significantly differ from the other concentrations. Fish survival rates were high, with no significant differences among the treatments. Therefore, it was concluded that the optimal concentration of red spinach extract for enhancing guppy color brightness was 5%
鱼类的颜色亮度受食物摄入的影响,但之前没有研究在鱼类饲料中使用红色菠菜提取物来提高颜色亮度和促进孔雀鱼(Poecilia reticulata)的生长。因此,本研究旨在确定红菠菜提取物的适宜浓度——三色苋的色泽亮度、生长发育和孔雀鱼的成活率。该实验采用完全随机设计,在北苏门答腊省北宾贾县的UPTD鱼类养殖中心复制三次三种浓度的红菠菜提取物(3%、4%和5%)。此外,将红菠菜提取物与含有41%粗蛋白的商业日粮混合,并以其体重的3%的比例每天给鱼服用40天。结果表明,不同剂量的红菠菜提取物对孔雀鱼的色泽亮度有显著影响(P<0.05),但对增重和鱼类存活率无显著影响(p<0.05)。类似地,在5%的浓度下观察到最高的鱼体重增加,这与其他浓度没有显著差异。鱼类存活率很高,不同处理之间没有显著差异。因此,得出结论,红菠菜提取物提高孔雀鱼颜色亮度的最佳浓度为5%
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引用次数: 0
Haematological effect of chlorfenapyr-exposed freshwater African mud catfish, Clarias gariepinus (Burchell 1822) 接触氯虫那吡的淡水非洲泥鲶的血液学效应
Pub Date : 2023-02-25 DOI: 10.13170/ajas.8.1.21296
I. K. Esenowo, M. Chukwu, A. U. Nelson, C. Alimba, M. Johnson, M. U. Effiong, A. U. Akpan, A. Ugwumba, N. I. Udoidung, N. C. Ukpong, A. O. Ugwumba, E. Ogidiaka, Adeyemi O.A. Ale, Godsgift Sunday
The effects of chlorfenapyr pesticide were investigated on juvenile of Clarias gariepinus. Sub-lethal test was carried out using 5, 7, 9, 11 and 15 mgL-1 of chlorfenapyr pesticide for 96 hours, and peripheral blood was collected through the caudal vein and examined for haematological changes. There was significant reduction (P0.05) in WBC when compared to the control. RBC increase was directly proportional to increase in chlorfenapyr dose and significantly differed from control (p0.05). HGB increased in a dose-dependent manner. MCV values of all treatment groups, except 15 mgL-1, were higher when compared to control. MCH was not significantly different from control. PLT decreased with increase in concentration and was lower for all treatment when compared with control. Results obtained in this study suggest that exposure to sub-lethal concentrations of chlorfenapyr can alter the haematological indices of non-target organisms.
研究了杀虫剂氯虫腈对加里皮Clarias幼鱼的影响。使用5、7、9、11和15mgL-1的氯芬那吡杀虫剂进行亚致死试验96小时,通过尾静脉采集外周血并检查血液学变化。与对照组相比,白细胞明显减少(P<0.05)。红细胞的增加与氯芬那吡剂量的增加成正比,与对照组有显著差异(p0.05)。除15mgL-1外,所有治疗组的MCV值均高于对照组。MCH与对照组无显著差异。PLT随着浓度的增加而降低,并且与对照组相比,所有治疗的PLT都较低。这项研究的结果表明,暴露于亚致死浓度的氯芬那吡会改变非目标生物的血液学指标。
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引用次数: 0
Blood profiles of African catfish Clarias gariepinus fingerlings fed diets supplemented with three strains of Lactobacillus plantarum 添加三株植物乳杆菌的非洲鲶鱼种鱼的血液分析
Pub Date : 2022-12-24 DOI: 10.13170/ajas.7.3.24925
D. Diyaolu, E. A. Fasakin, O. T. Adebayo
A 10-week feeding trial was carried out to evaluate strain-specific effects of Lactobacillus plantarum on blood profiles of African catfish Clarias gariepinus fingerlings. A catfish diet was formulated and divided into five portions (experimental diets). The first portion served as control (with no probiotic added to it, labeled as NP) while the second portion received 2.5g kg-1 multi-species commercial probiotics (CP). The third, fourth and fifth portions were the experimental diets that contained 2×109cfu/g of L. plantarum strains of ascension numbers LC333558, LC333559 and LC333560, and labeled as LP58, LP59 and LP60, respectively. Fifteen apparently healthy C gariepinus fingerlings (4.86±0.59g) were stocked in an experimental tank of 70 litres capacity, while the experimental feed was fed to the fish in triplicate tanks. Similar rearing conditions were maintained in all experimental tanks. The results showed significant (p0.05) difference between the fish fed control diet and probiotics supplementation diets in some of the haematological parameters examined. Probiotics supplementations did not affect total and differential leukocytes among different experimental groups. Except for RBC counts, fish groups fed with strains LB59 and LB60 had similar effects and appeared to have better haematological values than groups fed with LB58. Strains-specific effect of L. planatrum was also observed in creatinine levels among the experimental groups. Hence, it can be concluded that strains of L. plantarum affected some haematological parameters of C. gariepinus fingerlings positively, while dietary supplementation of L. plantarum LC333559 strain could be employed to improve haematological parameters of C. gariepinus fingerlings.
进行了为期10周的饲养试验,以评估植物乳杆菌对非洲鲶鱼Clarias gariepinus鱼种血液特征的菌株特异性影响。制定鲶鱼饮食,并将其分为五份(实验饮食)。第一部分作为对照(未添加益生菌,标记为NP),而第二部分接受2.5g kg-1多物种商业益生菌(CP)。第三份、第四份和第五份是实验日粮,含有2×109cfu/g的植物乳杆菌菌株,其升腾编号分别为LC333558、LC333559和LC333560,并分别标记为LP58、LP59和LP60。将15只明显健康的加里埃皮努斯鱼种(4.86±0.59g)储存在容量为70升的实验箱中,同时将实验饲料在一式三份的试验箱中喂给鱼。在所有实验槽中都保持了类似的饲养条件。结果显示,鱼类喂养的对照日粮和补充益生菌的日粮在某些血液学参数方面存在显著差异(p0.05)。益生菌补充剂不会影响不同实验组的总白细胞和差异白细胞。除了红细胞计数外,喂食菌株LB59和LB60的鱼类组具有相似的效果,并且似乎比喂食菌株LB58的组具有更好的血液学值。在实验组的肌酸酐水平中也观察到平坦乳杆菌的菌株特异性作用。因此,可以得出结论,植物乳杆菌菌株对加里epinus鱼种的某些血液学参数有积极影响,而日粮补充植物乳杆菌LC333559菌株可以改善加里epinu斯鱼种的血液学参数。
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引用次数: 0
Assessment of length-weight relationship and condition factor of Synodontics omias (Cuvier, 1816) in River Komadougou, Northeast, Nigeria 尼日利亚东北部科马杜古河Synodotics omias(Cuvier,1816)的长重关系和条件因子评估
Pub Date : 2022-12-20 DOI: 10.13170/ajas.7.3.27215
Ashley-Dejo S. Segun, Idi-Ogede M. Abubakar, Mohammed Sabo, M. Musa, Ogah S. Ijabo, Ogo M. Prince, Isaku Lucy
Length-weight relationship and condition factor of Synodontics omias (Cuvier, 1816) was studied between June to November 2020 in River Komadougou, Northeast, Nigeria. A total of 350 fish species were collected from artisanal fishermen at selected landing sites within the study area. The morphometric and meristic characteristics examined varies whereas some meristic features such as pelvic and dorsal fin spine had a constant value of 1 each. The mean condition factor shows no significant difference (P 0.05) throughout the sampling period. A linear relationship was established W = -0.82L1.91 and significant at P 0.01 with b value indicating a negative allometric growth pattern.  The correlation coefficient (r2 = 0.7904) indicated that LWR model is reliable. This study provides baselines on length-weight relationship and condition factor of Synodontics omias in River Komadougou, thus further research should be encouraged on the water body for sustainable utilization, decision making and policy formulation.
2020年6月至11月,在尼日利亚东北部的科马杜古河研究了Synodotics omias(Cuvier,1816)的长重关系和条件因子。在研究区域内选定的登陆点,共从个体渔民那里采集了350种鱼类。所检查的形态计量学和分生组织特征各不相同,而一些分生组织的特征,如骨盆和背鳍棘,其值各为1。平均条件因子在整个采样期内无显著差异(P 0.05)。建立了线性关系W=-0.82L1.91,在P 0.01时显著,b值表示负异速生长模式。相关系数(r2=0.7904)表明LWR模型是可靠的。本研究为科马杜沟河Synodotics omias的长重关系和条件因子提供了基线,因此应鼓励对水体进行进一步研究,以实现可持续利用、决策和政策制定。
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引用次数: 1
Genotoxic potentials of some selected heavy metals exposure on Clarias gariepinus (Burchell, 1822) and Oreochromis niloticus (Linnaeus, 1758) using RAPD-PCR technique 应用RAPD-PCR技术研究某些重金属暴露对加里皮Clarias gariepinus(Burchell,1822)和尼罗罗非鱼Oreochromis niloticus(Linnaeus,1758)的遗传毒性
Pub Date : 2022-12-20 DOI: 10.13170/ajas.7.3.22865
Fish health and the aquatic ecosystem are strongly interwoven and interrelated. The aquatic ecosystem receives a range of anthropogenic chemicals which have toxicological or lethal health effects on the aquatic animals. The aim of this study was to determine the level of induced mutation and genomic stability of different sub-lethal doses (25%, 50% and 75% LC50) of heavy metal [Nickel (Ni), Mercury (Hg), Lead (Pb)and Zinc (Zn)] in Clarias gariepinus (Burchell, 1822) andOreochromis niloticus (Linnaeus, 1758) using RAPD-PCR technology over a period of 21 days. Four highly polymorphic RAPD markers were evaluated on isolated DNA from both heavy metal exposed fishes and control fishes after exposure. Observation of RAPD profiles in C. gariepinus revealed more damaging effect as sub lethal doses increased with zinc (a + b = 44 bands) mercury (a + b = 41 bands) nickel (a + b = 37 bands) lead (a + b = 35 bands) than with nickel (a + b = 37 bands) zinc (a + b = 34 bands) lead (a + b = 32 bands) mercury (a + b = 31 bands) for O. niloticus when compare to the control groups. Although, the genomic stability template decreased sub lethal heavy metal doses, higher stability was observed in O. niloticus (GTSPb = 26.32% GTSHg = 18.42% GTSZn = 10.53% GTSNi = 2.63%) than in C. gariepinus (GTSPb = 5.41% GTSNi = 0.0% GTSHg = -10.81% GTSZn = -18.92%). The results obtained showed differential variation in heavy metal induced   genetic mutation and genomic stabilty in C. gariepinus and O. niloticus. These observable differences might be due to the physiological structure of the fish species evaluated. This study also confirms that RAPD–PCR technology is a useful tools in detecting the genotoxic effect of heavy metals in aquatic organisms but due to low reproducibility of RAPD results, it is recommended that this technology should be used along with other molecular techniques in fish genotoxicity studies.Keywords: Heavy metals, Induced, Genotoxicity, Fish, RAPD-PCR Technique
鱼类健康和水生生态系统是紧密交织和相互关联的。水生生态系统接收一系列对水生动物具有毒理学或致命健康影响的人为化学物质。本研究的目的是使用RAPD-PCR技术在21天内确定不同亚致死剂量(25%、50%和75%LC50)的重金属[镍(Ni)、汞(Hg)、铅(Pb)和锌(Zn)]在保加利亚克拉里昂(Burchell,1822)和尼罗罗非鱼(Linnaeus,1758)中的诱导突变水平和基因组稳定性。对重金属暴露鱼和对照鱼的分离DNA进行了4个高度多态性的RAPD标记的鉴定。C.的RAPD图谱观察。对于O,随着亚致死剂量的增加,锌(a+b=44条带)汞(a+b=41条带)镍(a+b=37条带)铅(a+b=35条带)的gariepinus表现出更大的破坏作用。与对照组相比。尽管基因组稳定性模板降低了亚致死重金属剂量,但在O。niloticus(GTSPb=26.32%GTSHg=18.42%GTSZn=10.53%GTSNi=2.63%)和C.gariepinus(GTSPb=5.41%GTSNi=0.0%GTSHg=-10.81%GTSZn=-18.92%)相比。gariepinus和O。尼洛蒂库斯。这些可观察到的差异可能是由于所评估的鱼类的生理结构造成的。本研究还证实,RAPD–PCR技术是检测重金属对水生生物遗传毒性作用的有用工具,但由于RAPD结果的可重复性低,建议将该技术与其他分子技术一起用于鱼类遗传毒性研究。关键词:重金属;诱导;遗传毒性;鱼类;RAPD-PCR技术
{"title":"Genotoxic potentials of some selected heavy metals exposure on Clarias gariepinus (Burchell, 1822) and Oreochromis niloticus (Linnaeus, 1758) using RAPD-PCR technique","authors":"","doi":"10.13170/ajas.7.3.22865","DOIUrl":"https://doi.org/10.13170/ajas.7.3.22865","url":null,"abstract":"Fish health and the aquatic ecosystem are strongly interwoven and interrelated. The aquatic ecosystem receives a range of anthropogenic chemicals which have toxicological or lethal health effects on the aquatic animals. The aim of this study was to determine the level of induced mutation and genomic stability of different sub-lethal doses (25%, 50% and 75% LC50) of heavy metal [Nickel (Ni), Mercury (Hg), Lead (Pb)and Zinc (Zn)] in Clarias gariepinus (Burchell, 1822) andOreochromis niloticus (Linnaeus, 1758) using RAPD-PCR technology over a period of 21 days. Four highly polymorphic RAPD markers were evaluated on isolated DNA from both heavy metal exposed fishes and control fishes after exposure. Observation of RAPD profiles in C. gariepinus revealed more damaging effect as sub lethal doses increased with zinc (a + b = 44 bands) mercury (a + b = 41 bands) nickel (a + b = 37 bands) lead (a + b = 35 bands) than with nickel (a + b = 37 bands) zinc (a + b = 34 bands) lead (a + b = 32 bands) mercury (a + b = 31 bands) for O. niloticus when compare to the control groups. Although, the genomic stability template decreased sub lethal heavy metal doses, higher stability was observed in O. niloticus (GTSPb = 26.32% GTSHg = 18.42% GTSZn = 10.53% GTSNi = 2.63%) than in C. gariepinus (GTSPb = 5.41% GTSNi = 0.0% GTSHg = -10.81% GTSZn = -18.92%). The results obtained showed differential variation in heavy metal induced   genetic mutation and genomic stabilty in C. gariepinus and O. niloticus. These observable differences might be due to the physiological structure of the fish species evaluated. This study also confirms that RAPD–PCR technology is a useful tools in detecting the genotoxic effect of heavy metals in aquatic organisms but due to low reproducibility of RAPD results, it is recommended that this technology should be used along with other molecular techniques in fish genotoxicity studies.Keywords: Heavy metals, Induced, Genotoxicity, Fish, RAPD-PCR Technique","PeriodicalId":52680,"journal":{"name":"Aceh Journal of Animal Science","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48360811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effects of lactic acid bacteria on phytate and the ensiling properties of Sorghum bicolor L. Moench 乳酸菌对双色高粱植酸酶及青贮性能的影响
Pub Date : 2022-12-20 DOI: 10.13170/ajas.7.3.25050
Mugoti Alban, Chikumba Nation, Munengwa Anderson, Dziwanyika Lenin
Sorghum bicolor is known for its multiple uses and is grown for human grain and livestock feed. The study aimed at assessing the effects of incorporating Lactic Acid bacteria (LAB) on the ensiling properties of sorghum bicolor and an antinutritional factor (ANF) called phytate. Dry matter, pH, ash, crude protein, crude fiber, carbohydrates, and phytate were measured as indices of the grain ferment value from January to August. A feed nutrient quality evaluation was conducted with 3 treatments and having 4 replications. The test was conducted using a completely random design. Laboratory analysis of the variables was done on day 6 of the experiment. Results showed that treating sorghum with LAB significantly (P0.05) affected the nitrogen-free extracts, pH, and phytate but did not however affect the dry matter, crude fiber, crude protein, and ash content (P0.05). There was a significant (p0.05) decrease in phytate and nitrogen-free extracts for the LAB-treated sorghum as compared to non-LAB-treated sorghum. Ash content was significantly lower (p0.05) in LAB-treated sorghum and interestingly higher in non-LAB-treated sorghum. The study showed that treating sorghum with LAB significantly reduces phytate levels in feed and has little effect on the nutrient status of the feed.Keywords: Sorghum, Proximate, Phytate, LAB-treated
双色高粱以其多种用途而闻名,被种植用于人类谷物和牲畜饲料。本研究旨在评估掺入乳酸菌(LAB)对高粱双色和一种名为植酸酶的抗营养因子(ANF)的青贮性能的影响。以干物质、pH值、灰分、粗蛋白质、粗纤维、碳水化合物和植酸酶为指标,测定了1~8月粮食发酵值。用3个处理和4次重复进行饲料营养质量评价。试验采用完全随机设计进行。在实验的第6天对变量进行实验室分析。结果表明,经LAB处理的高粱对无氮提取物、pH值和植酸酶的影响显著(P0.05),但对干物质、粗纤维、粗蛋白和灰分的影响不大(P0.05)。LAB处理的高粱灰分含量显著较低(p0.05),而非LAB处理高粱灰分含量更高。研究表明,LAB处理高粱显著降低了饲料中的植酸酶水平,对饲料的营养状况影响不大。关键词:高粱,邻苯二甲酸酯,乳酸菌处理
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引用次数: 1
The impact of avian IgY antibodies and probiotics supplementation via drinking water on growth and laying performance of 16-week-old layer pullets 饮水补充鸡IgY抗体和益生菌对16周龄蛋鸡生长和产蛋性能的影响
Pub Date : 2022-12-20 DOI: 10.13170/ajas.7.3.26592
Neil P.L. Medina, L. Purnamasari, Joseph P. Olarve, J. D. dela Cruz
Antibiotics raise an issue concerning the development of antibiotic resistance of some pathogenic bacteria. The use of probiotics and Avian IgY in layer pullet farming is a better way of protecting against pathogenic bacteria and increasing production performance. Supplementation of Avian IgY antibodies and probiotics(Lactobacillus acidophilus, Streptococcus faecium, Bifido bacterium longghum, Bacillus thermophilum) were conducted on 5,724 healthy layer pullets to determine its effect on their growth and laying performance. The supplement was in powder form with a dosage of 100 mg per liter of water for 6 hours. The effect of the supplementation was evaluated by measuring production parameters on growth and laying performance of the layer pullets; there were 3 treatment groups, the control group, Treatment 2 which received 5 days initial supplementation for the first week and then twice every week for 9 weeks and Treatment 3 which received 5 days initial supplementation for the first week and then twice every 2 weeks for 9 weeks. Results showed that the supplementation had significant effects in the age in days when 50% of the total population per treatment laid their first eggs, average number of eggs produced, % Hen day production, % Hen house production, total weight of eggs, and feed conversion efficiency. It also showed that the supplementation is economical and yielded higher profit. On the other hand, the supplementation had no significant effect on the average body weight and mortality count of the layer pullets. The supplementation of Avian IgY antibodies and probiotics in water soluble powder via drinking water with 5 days initial supplementation of Avian IgY antibodies and probiotics for the first week; then twice every 2 weeks for 9 weeks has the greatest effects to increase the production performance and profitability of layers.Keywords:  Avian IgY antibodies, Growth performance, Laying performance, Layer pullets, Probiotics   
抗生素引起了一些致病菌对抗生素产生耐药性的问题。在蛋鸡养殖中使用益生菌制剂和禽IgY是防治病原菌和提高生产性能的较好方法。本试验在5724只健康蛋鸡上添加了禽IgY抗体和益生菌(嗜酸乳杆菌、粪链球菌、长沟双歧杆菌、嗜热芽孢杆菌),以观察其对蛋鸡生长和产蛋性能的影响。该补充剂为粉末形式,剂量为每升水100毫克,持续6小时。通过测定生产参数对蛋鸡生长和产蛋性能的影响,评价添加效果;有3个治疗组,对照组,治疗2第一周初始补充5天,然后每周补充2次,连续9周,治疗3第一周初始补充5天,然后每2周补充2次,连续9周。结果表明,饲粮添加对每处理50%的首产蛋日龄、平均产蛋数、母鸡日产蛋率、母鸡舍产蛋率、鸡蛋总重和饲料转化率均有显著影响。结果表明,该方法具有经济性和较高的效益。另一方面,饲粮添加量对蛋鸡平均体重和死亡率无显著影响。通过饮水方式在水溶性粉中添加禽IgY抗体和益生菌,第一周初始添加禽IgY抗体和益生菌5 d;每2周饲喂2次,连续饲喂9周,对提高蛋鸡生产性能和盈利能力的效果最大。关键词:禽IgY抗体,生长性能,产蛋性能,蛋鸡,益生菌
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引用次数: 1
Growth performance and histological response of Clarias gariepinus raised on unprocessed dried leaves of Telfairia occidentalis and Mucuna utilis 在西芹和麻瓜未加工干叶上饲养的加里宾Clarias gariepinus的生长性能和组织学反应
Pub Date : 2022-12-20 DOI: 10.13170/ajas.7.3.25836
A. O. Ajiboye, A. S. Tola-Fabunmi, A. O. Ayanboye
Nutrient utilization is determined by absorbable and digestible nutrients in fish feeds. The aim of the experiment was to investigate the effects of unprocessed dried leaves of Telfairia occidentalis (UDLTO) and Mucuna utilis (UDLMU) on histology and growth of C. gariepinus.  Sixteen weeks feeding trial experiment was conducted using two hundred and twenty-five Clarias gariepinus with a mean weight 4.16±0.09g. Fish meal was replaced with 0, 25, 50, 75 and 100% levels of UDLMU and UDLTO. Growth parameters and histological examinations were carried out according to standard procedures. Mean Weight Gain (MWG) of 0% (15.8g) was significantly higher P0.05 compared to other treatments. Specific Growth Rate (SGR) of 0% (0.64) varied significantly P0.05 than other levels of inclusions. Kidneys of fish fed 25 and 50% graded levels showed patchy vacuolar degeneration of the renal tubules. Gills of fish fed 75 and 100% graded levels showed diffuse capillaries and moderate congestion of lamellar in the gills. Tissue examinations of fish fed 50, 75 and 100% graded levels exhibited clinical signs of moderate atrophy and vacuolation of hepatocytes and diffuse hepatocellular swelling and degeneration in the liver. Histological investigations revealed fragmentation and atrophy of muscle fibre in fish fed 75% graded level. There was moderate atrophy of villi of the intestine of fish fed 100% graded level. It is apparent that the increased levels of UDLMU and UDLTO could not support good growth as presumed due to the presence of anti-nutritional factors in the leaves.Keywords: Nutrient utilization, Fish meal, Inclusions, standard procedures, Clinical signs
营养物质的利用取决于鱼类饲料中可吸收和可消化的营养物质。本试验旨在研究未加工的西花蓟马(Telfairia occidentalis, UDLTO)和牛粪(Mucuna utilis, UDLMU)干叶对加里平棘球蜂(cariepinus)组织学和生长的影响。选取平均体重4.16±0.09g的加里平克拉尾鱼225尾,进行16周饲养试验。用0、25、50、75和100%的UDLMU和UDLTO替代鱼粉。按照标准程序进行生长参数和组织学检查。平均增重(MWG)为0% (15.8g),显著高于其他处理(P0.05)。特定生长率(SGR)为0%(0.64),与其他水平的包裹体差异显著(P0.05)。饲喂25%和50%分级水平的鱼肾脏显示肾小管斑块状空泡变性。饲喂75%和100%分级水平的鱼鳃毛细血管弥散,片层有中度充血。饲喂50%、75%和100%分级水平的鱼的组织检查显示肝细胞中度萎缩和空泡化,肝脏弥漫性肝细胞肿胀和变性的临床症状。组织学研究显示,75%分级饲喂水平的鱼肌纤维出现断裂和萎缩。饲喂100%分级饲料的鱼肠绒毛出现中度萎缩。很明显,由于叶片中存在抗营养因子,UDLMU和UDLTO水平的增加不能像假设的那样支持良好的生长。关键词:营养利用,鱼粉,夹杂物,标准程序,临床体征
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引用次数: 0
Effect of adding onion oil and seeds to local female goose (Anserinea) diet on the levels of antioxidants enzyme and sex hormones 地方鹅日粮中添加洋葱油和洋葱籽对抗氧化酶和性激素水平的影响
Pub Date : 2022-08-09 DOI: 10.13170/ajas.7.3.25872
This study was in the scientific research field for the Babylon Provence period from 3/1/2018 to 20/4/2018 (105 days) to know the effect of adding different levels of onion oil and seeds to Iraqi local goose rations on some antioxidants and levels of sex hormones, such as it was used in This study: 75 sexually mature female geese, and at the beginning of the production stage, were divided into five treatments, and for each treatment 15 females divided into three replicates / each replicate had 5 females. The experimental treatments were as follows: The first treatment (T1) was a control treatment without any addition, the second treatment (T2) was added to onion seed powder at an amount of 1.5 gm per kg of feed, the third treatment (T3) was added to onion seed powder at an amount of 3 gm per kg of feed, The fourth treatment (T4) was added to onion seed oil at an average of 0.10 ml per kg of feed, while the fifth treatment (T5) was added to it onion seed oil at an average of 0.20 ml per kg of feed and these percentages were added from the beginning of the experiment until the end of the experiment period. Blood was drawn from the experimental birds from the jugular vein weekly for the purpose of measuring the concentrations of MDA, GSH-px, SOD and the level of the hormones Progesterone and Estrogen and then according to an average every three weeks to present the results of the study on the basis of five periods of length, each of 21 days, the results of the study indicated a decrease Significant in the oxidative enzyme activity understudy in the addition treatments compared to the control treatment, also the sex hormones took the same curve of significant improvement of the additional treatments compared to the control treatment.Keywords: Antioxidants; Local goose; Onion oil; Onion seeds; Sex hormones 
这项研究于2018年3月1日至2018年4月20日(105天)在巴比伦-普罗旺斯时期的科学研究领域进行,以了解在伊拉克当地鹅的口粮中添加不同水平的洋葱油和种子对一些抗氧化剂和性激素水平的影响,如本研究中使用的:75只性成熟的母鹅,被分为五个处理,并且对于每个处理,15只雌性被分为三个重复/每个重复有5只雌性。实验处理如下:第一个处理(T1)是不添加任何添加剂的对照处理,第二个处理(T2)以每公斤饲料1.5克的量添加到洋葱籽粉末中,第三个处理(T3)以每千克饲料3克的量加入到洋葱籽粉中,第四个处理(T4)以平均每公斤饲料0.10毫升的量加入洋葱籽油中,而第五处理(T5)以平均每公斤饲料0.20ml的洋葱籽油添加到其中,并且这些百分比从实验开始直到实验期结束添加。每周从实验鸟的颈静脉抽血,以测量MDA、GSH-px、SOD的浓度以及激素孕酮和雌激素的水平,然后根据平均每三周一次的结果,研究结果表明,与对照处理相比,添加处理的氧化酶活性替补显著降低,性激素也呈现出与对照处理相同的显著改善曲线。关键词:抗氧化剂;当地鹅;洋葱油;洋葱籽;性激素
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引用次数: 0
DNA barcoding and morphometric of Rastrelliger spp. in North Maluku Sea, Indonesia 印度尼西亚北马鲁古海拉斯特利格氏种的DNA条形码和形态计量学研究
Pub Date : 2022-08-09 DOI: 10.13170/ajas.7.3.26530
N. Akbar, E. Wibowo, Muhammad Irfan, M. J. Achmad, I. Wahab, Y. Ramili, R. E. Paembonan, Abdurachman Baksir, D. Bengen, Armalita Tahir, F. Ismail, N. Najamuddin, I. Marus, A. A. Siolimbona, A. Toha, I. Nurjaya, N. M. Natih, B. Subhan
High exploration activity is feared to have an impact to mackerel populations. A sustainable management approach should be taken to provide information about the status of mackerel populations. Study of mackerel population status can be carried out through genetic information. The DNA of the fish samples were collected at traditional fish markets (Morotai, Bacan and Ternate). Laboratory works such as extraction, amplification, electrophoresis and DNA sequencing were analysis at the Indonesian Biodiversity Laboratory (Bionesia). The molecular characteristics of Rastrelliger kanagurta were 374 base pairs (bp). The composition of nucleotides showed the similarity of frequencies between species. Phylogenetic relationship of R. kanagurta in North Maluku Sea suggested that there was any differentiation. The genetic diversity of R. kanagurta was high with a total number of haplotypes and diverse nucleotide diversity. The minimum spawning networks (MSN) found 5 haplotype networks from a total of 12 samples. Morphological measurements of standard length, head height, body width, pectoral fin length and tail were found to have variable values. The length of the weight of the fish is obtained of b = 3, indicating that the growth pattern was isometric or weight gain was equivalent to the growth of the fish length.Keywords: Biodiversity, conservation, ecology, morphology, species
人们担心高勘探活动会对鲭鱼种群产生影响。应采取可持续的管理方法,提供关于鲭鱼种群状况的信息。可以通过遗传信息来研究鲭鱼种群状况。鱼类样本的DNA是在传统的鱼类市场(Morotai、Bacan和Ternate)采集的。印尼生物多样性实验室(Bionesia)对提取、扩增、电泳和DNA测序等实验室工作进行了分析。卡那古氏Rastrelliger的分子特征为374个碱基对(bp)。核苷酸的组成显示了物种之间频率的相似性。北马鲁古海卡那古尔塔的系统发育关系表明存在分化。卡那古尔塔的遗传多样性较高,具有总的单倍型数和多样的核苷酸多样性。最小产卵网络(MSN)从总共12个样本中发现了5个单倍型网络。标准长度、头部高度、身体宽度、胸鳍长度和尾部的形态学测量值各不相同。鱼的体重长度为b=3,表明生长模式是等距的,或者体重增加相当于鱼的长度增长。关键词:生物多样性、保护、生态学、形态学、物种
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引用次数: 1
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Aceh Journal of Animal Science
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