Human and livestock population increase has led to escalating prices of energy feed stuffs used in aquaculture. There have been some researches on various inexpensive agro-industrial by-products as substitutes for maize in fish diets with varying degrees of success. This study investigated the growth performance of Oreochromis niloticus juveniles fed with autoclaved Mango seed formulated fish diet as a dietary energy source at different inclusion level. 150 Oreochromis niloticus juveniles with mean weight of 10.24±0.05g were randomly allocated to five dietary treatments (T1, T2, T3, T4, T5). The fish were acclimated for two weeks and were allotted to five treatments in plastic tanks (26x46x20cm). Each treatment contained 10 fish per tank. The fish were fed experimental diets at T1:0%, T2: 25%, T3: 50%, T4: 75% and T5: 100% Mango Seed Kernel Meal (MSKM) inclusion levels respectively. The highest Mean weight Gain (MWG) and lowest Feed Conversion Ratio (FCR) was recorded in Treatment 2: 25% (48.93±4.88); (0.91±0.07) while the lowest MWG and highest FCR was recorded in Treatment 5: 100% (27.30±3.99); (1.47±0.16). Specific Growth Rate (SGR) was lower in Treatment 5: 100% when compared to other treatments. Feed intake followed a decreasing order. The blood samples collected showed that there was significant differences (p0.05) In conclusion, growth performance and haematological activity could be best improved by substituting mango seed kernel meal at 75% with no implication on growth and fish health.
{"title":"Performance of Oreochromis niloticus juveniles fed autoclaved mango seed kernel diets","authors":"Falaye Augustine Eyiwunmi, Sule Okanlawon Shakiru, Sanogo Salimata, Kechicha Olufunmike Martha","doi":"10.13170/AJAS.6.2.19553","DOIUrl":"https://doi.org/10.13170/AJAS.6.2.19553","url":null,"abstract":"Human and livestock population increase has led to escalating prices of energy feed stuffs used in aquaculture. There have been some researches on various inexpensive agro-industrial by-products as substitutes for maize in fish diets with varying degrees of success. This study investigated the growth performance of Oreochromis niloticus juveniles fed with autoclaved Mango seed formulated fish diet as a dietary energy source at different inclusion level. 150 Oreochromis niloticus juveniles with mean weight of 10.24±0.05g were randomly allocated to five dietary treatments (T1, T2, T3, T4, T5). The fish were acclimated for two weeks and were allotted to five treatments in plastic tanks (26x46x20cm). Each treatment contained 10 fish per tank. The fish were fed experimental diets at T1:0%, T2: 25%, T3: 50%, T4: 75% and T5: 100% Mango Seed Kernel Meal (MSKM) inclusion levels respectively. The highest Mean weight Gain (MWG) and lowest Feed Conversion Ratio (FCR) was recorded in Treatment 2: 25% (48.93±4.88); (0.91±0.07) while the lowest MWG and highest FCR was recorded in Treatment 5: 100% (27.30±3.99); (1.47±0.16). Specific Growth Rate (SGR) was lower in Treatment 5: 100% when compared to other treatments. Feed intake followed a decreasing order. The blood samples collected showed that there was significant differences (p0.05) In conclusion, growth performance and haematological activity could be best improved by substituting mango seed kernel meal at 75% with no implication on growth and fish health.","PeriodicalId":52680,"journal":{"name":"Aceh Journal of Animal Science","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45782530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Growth and all activities of fish depend significantly on the food they consume. However, with the rising price of conventional feedstuffs, there is a need to investigate less expensive and readily available plant source as an additive. This research is designed to study the effect of dietary Aloe vera on the growth performance of Oreochromis niloticus juveniles (41.71±0.78g) and its effect on genotoxicity (MN: Micronucleus; LB: Lobed; BD: Bud; BL: Blebbed; BN: Binucleated; NT: Notched). This research investigated the growth performance and genotoxicity activity of Oreochromis niloticus juveniles in a tank culture system. Aloe vera (fine powder) was used as a test ingredient in the feed formulation at five inclusion levels (T1 = 5g, T2 =10g, T3 =15g, T4 =20g, T5 =25g) and the Control diet (C0) without the test ingredient. Test diets were fed to Oreochromis niloticus juveniles, and each diet was assigned to the treatments and control overall in triplicates. Feeding with test diets was for twelve (12) weeks at 5% of their total body weight daily, which was divided into two and administered at 8 hrs and 16 hrs. The result showed that the highest growth rate (10.99 ± 5.49g) and the lowest FCR (0.68 ± 0.08) were recorded in the fish fed with the Control diet (C0). Better growth and nutrient utilization were achieved at low inclusion levels of Aloe vera, at 5g(T1) (8.98 ± 4.49) and 10g (T2) (6.73 ± 3.28) compared to the higher levels of incorporations of the test ingredient. The mean pH and temperature (°C) and dissolved Oxygen level mg/L are 6.50±0.30, 26.3±0.60 and 4.48±0.52mg/L respectively. The values of micronuclei for the cultured Oreochromis niloticus juveniles were recorded to be (T1 = 5.50 ± 0.50, T2 = 2.00 ± 1.00, T3 = 2.00 ± 0.00, T4 = 2.50 ± 1.50, T5 = 4.00 ± 1.00, and C0 = 7.00 ± 2.00). Nuclear abnormalities were recorded in T2 (BD = 1.00 ± 0.00), T3 (NT = 1.00 ± 0.00), T4 (BD = 1.33 ± 0.47, BN = 3.33 ± 0.47), and T5 (BN = 5.33 ± 0.47, LB = 3.00 ± 1.00, BL = 1.67 ± 0.47) . However, no abnormality was recorded in the fish fed with the Control diet (C0). This result showed that the slight addition of this Aloe vera to the diet of Oreochromis niloticus would enhance productivity.
{"title":"The growth performance and genotoxicity effect of dietary Aloe vera on Oreochromis niloticus juveniles","authors":"S. O. Ayoola, H. Ishola","doi":"10.13170/AJAS.5.2.16044","DOIUrl":"https://doi.org/10.13170/AJAS.5.2.16044","url":null,"abstract":"Growth and all activities of fish depend significantly on the food they consume. However, with the rising price of conventional feedstuffs, there is a need to investigate less expensive and readily available plant source as an additive. This research is designed to study the effect of dietary Aloe vera on the growth performance of Oreochromis niloticus juveniles (41.71±0.78g) and its effect on genotoxicity (MN: Micronucleus; LB: Lobed; BD: Bud; BL: Blebbed; BN: Binucleated; NT: Notched). This research investigated the growth performance and genotoxicity activity of Oreochromis niloticus juveniles in a tank culture system. Aloe vera (fine powder) was used as a test ingredient in the feed formulation at five inclusion levels (T1 = 5g, T2 =10g, T3 =15g, T4 =20g, T5 =25g) and the Control diet (C0) without the test ingredient. Test diets were fed to Oreochromis niloticus juveniles, and each diet was assigned to the treatments and control overall in triplicates. Feeding with test diets was for twelve (12) weeks at 5% of their total body weight daily, which was divided into two and administered at 8 hrs and 16 hrs. The result showed that the highest growth rate (10.99 ± 5.49g) and the lowest FCR (0.68 ± 0.08) were recorded in the fish fed with the Control diet (C0). Better growth and nutrient utilization were achieved at low inclusion levels of Aloe vera, at 5g(T1) (8.98 ± 4.49) and 10g (T2) (6.73 ± 3.28) compared to the higher levels of incorporations of the test ingredient. The mean pH and temperature (°C) and dissolved Oxygen level mg/L are 6.50±0.30, 26.3±0.60 and 4.48±0.52mg/L respectively. The values of micronuclei for the cultured Oreochromis niloticus juveniles were recorded to be (T1 = 5.50 ± 0.50, T2 = 2.00 ± 1.00, T3 = 2.00 ± 0.00, T4 = 2.50 ± 1.50, T5 = 4.00 ± 1.00, and C0 = 7.00 ± 2.00). Nuclear abnormalities were recorded in T2 (BD = 1.00 ± 0.00), T3 (NT = 1.00 ± 0.00), T4 (BD = 1.33 ± 0.47, BN = 3.33 ± 0.47), and T5 (BN = 5.33 ± 0.47, LB = 3.00 ± 1.00, BL = 1.67 ± 0.47) . However, no abnormality was recorded in the fish fed with the Control diet (C0). This result showed that the slight addition of this Aloe vera to the diet of Oreochromis niloticus would enhance productivity. ","PeriodicalId":52680,"journal":{"name":"Aceh Journal of Animal Science","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46715068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Knowledge, attitudes, and practices (KAP) on antimicrobial use (AMU) of livestock farmers are poorly understood in Zimbabwe despite their essence in combating the development of antimicrobial resistance. The aim of the study was to assess these patterns based on suggested KAP variables. A questionnaire-based survey of (n=60) Small Scale Commercial Farmers (SSCF), (n=60) Large Scale Commercial farmers (LSCF), and (n=60) resettled farmers (A1) was undertaken from January to March 2019. Participants were purposively selected from 10 wards based on willingness to participate in the study. Logistic regression, analysis of variance, and Pearson's correlation were performed in SAS (2003) version 6. Overall AMU practice scores were above 50% despite detecting violation of manufacturer's specifications. AMU was correlated with farmer's knowledge, r (178) = 0.42, p =0.000 and attitude, r (178) = 0.54, p =0.000. Farming scale, type of livestock kept and level of education influenced AMU (P 0.05). Farming scale influenced above 50% of the farmers to change label dosages, withdrawal periods, treatment frequencies, and consult friends on AMU. A similar pattern was noted on the effect of main livestock species kept on changing treatment frequencies and the use of human antibiotics. AMU without prescriptions increased with a decreasing level of education (P = 0.010). Knowledge on AMU was high for LSCF (average 58%), low for A1 (average 33%), and SSCF (average 46%). Attitudes were positive for LSCF (average 67%) and SSCF (average 57%) and negative for A1 (average 49%). We concluded that there is a serious violation of antimicrobial manufacturer's specifications by farmers in the Chikomba district which is associated with poor knowledge and attitudes on prudent ways of AMU
{"title":"Perceptions and practices on antimicrobial use by the farmers of the Chikomba District, Zimbabwe","authors":"Anderson Munengwa, Chikumba Nation, Mugoti Alban","doi":"10.13170/AJAS.5.2.16713","DOIUrl":"https://doi.org/10.13170/AJAS.5.2.16713","url":null,"abstract":"Knowledge, attitudes, and practices (KAP) on antimicrobial use (AMU) of livestock farmers are poorly understood in Zimbabwe despite their essence in combating the development of antimicrobial resistance. The aim of the study was to assess these patterns based on suggested KAP variables. A questionnaire-based survey of (n=60) Small Scale Commercial Farmers (SSCF), (n=60) Large Scale Commercial farmers (LSCF), and (n=60) resettled farmers (A1) was undertaken from January to March 2019. Participants were purposively selected from 10 wards based on willingness to participate in the study. Logistic regression, analysis of variance, and Pearson's correlation were performed in SAS (2003) version 6. Overall AMU practice scores were above 50% despite detecting violation of manufacturer's specifications. AMU was correlated with farmer's knowledge, r (178) = 0.42, p =0.000 and attitude, r (178) = 0.54, p =0.000. Farming scale, type of livestock kept and level of education influenced AMU (P 0.05). Farming scale influenced above 50% of the farmers to change label dosages, withdrawal periods, treatment frequencies, and consult friends on AMU. A similar pattern was noted on the effect of main livestock species kept on changing treatment frequencies and the use of human antibiotics. AMU without prescriptions increased with a decreasing level of education (P = 0.010). Knowledge on AMU was high for LSCF (average 58%), low for A1 (average 33%), and SSCF (average 46%). Attitudes were positive for LSCF (average 67%) and SSCF (average 57%) and negative for A1 (average 49%). We concluded that there is a serious violation of antimicrobial manufacturer's specifications by farmers in the Chikomba district which is associated with poor knowledge and attitudes on prudent ways of AMU","PeriodicalId":52680,"journal":{"name":"Aceh Journal of Animal Science","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46583683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Y. Yonvitner, I. Setyobudiandi, R. Dahuri, J. Jamilah
Simping (P. placenta) is a coastal resource found in shallow water with a muddy substrate. Simping widely used as a source of food and as raw material for the decoration. Utilization of simping until now has not yet focused on recruitment, reproductive aspect. This information is important as a basis data for determining the size of the simping catch. This study aimed to find out about of reproduction aspect, determining sex ratio the size of the catch simping. This research was conduct for three months at 3 locations. The number of samples in the analysis is 36 species to determine sex ratio and gonad maturity. Sex ratio indifferent from male and female, but based on the time sex ratio of simping significant, both male and female. The length size of gonads shellfish matures form 5.50 cm and 5.85 cm at 2 TKG conditions. Length of simping mature from 6.08 cm, 6.24 cm, 6.45 cm and 7.11 cm dominant at TKG mature stage 3, and 8.61 cm dominant at 4 TKG conditions. Sex ratio reaches 6 cm shell length and not significant between males and females (M: F=1;1). It is concluded that the level of maturity of gonads increases with increasing of simping size.
{"title":"Gonad maturity of simping Placuna placenta, Linn 1758 (Bivalve: Placunidae) harvested from Kronjo Coastal, Indonesia","authors":"Y. Yonvitner, I. Setyobudiandi, R. Dahuri, J. Jamilah","doi":"10.13170/AJAS.5.1.14930","DOIUrl":"https://doi.org/10.13170/AJAS.5.1.14930","url":null,"abstract":"Simping (P. placenta) is a coastal resource found in shallow water with a muddy substrate. Simping widely used as a source of food and as raw material for the decoration. Utilization of simping until now has not yet focused on recruitment, reproductive aspect. This information is important as a basis data for determining the size of the simping catch. This study aimed to find out about of reproduction aspect, determining sex ratio the size of the catch simping. This research was conduct for three months at 3 locations. The number of samples in the analysis is 36 species to determine sex ratio and gonad maturity. Sex ratio indifferent from male and female, but based on the time sex ratio of simping significant, both male and female. The length size of gonads shellfish matures form 5.50 cm and 5.85 cm at 2 TKG conditions. Length of simping mature from 6.08 cm, 6.24 cm, 6.45 cm and 7.11 cm dominant at TKG mature stage 3, and 8.61 cm dominant at 4 TKG conditions. Sex ratio reaches 6 cm shell length and not significant between males and females (M: F=1;1). It is concluded that the level of maturity of gonads increases with increasing of simping size.","PeriodicalId":52680,"journal":{"name":"Aceh Journal of Animal Science","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43866924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
One of the important components in aquatic ecosystems is fish diversity, which is a key factor in determining its functionality. The coral reef fish is one of the aquatic biotas susceptible to pollutants. Besides being able to influence the composition of fish diversity, it also has an impact on its biological factors, such as its length-weight and conditions. This study aims to examine the relationship of length-weight and condition factors of four dominant fishes caught, which are ecologically and economically important, namely Lates calcarifer, Parachaetodon ocellatus, Stephanolepis cirrhifer, and Chelmon rostractus.These fish species were dominantly caught using bubu trap. The results of the length-weight relationship were b3 of all species, with an exponent value of 2.247 for the L. calcarifer; 1.932 for P. ocellatus; 2.74 for S. cirrhifer and 2.84 for C. rostractus. Fulton’s (K) condition factor for L. carifer ranged from 15.55-21.06 (average: 17.95), for P.ocellatus ranging from 27.30-35.59 (average: 30.38), for S. cirrhifer ranged from 17.58-27.23 (average: 21.29), and for C. rostractus ranging from 21.86-30.95 (average: 24.24). While the relative weight (Wr) condition factor average value of L. calcarifer is 102.53, P. ocellatus is 100.88, S. cirrhifer is 101.72, and C. rostractus is 101.11. It is concluded that the fish had an allometrict growth pattern, and condition factor values indicate good aquatic environmental conditions and a balance between prey and predatorKeywords: Length-Weight; Growth Pattern; Condition Factors
{"title":"Length-weight relationships and condition factors of four dominant fish caught by coral bubu trap on the west coast of Batam Island, Indonesia","authors":"Ramses Ramses, I. Ismarti, Fauziah Syamsi","doi":"10.13170/ajas.5.1.14902","DOIUrl":"https://doi.org/10.13170/ajas.5.1.14902","url":null,"abstract":"One of the important components in aquatic ecosystems is fish diversity, which is a key factor in determining its functionality. The coral reef fish is one of the aquatic biotas susceptible to pollutants. Besides being able to influence the composition of fish diversity, it also has an impact on its biological factors, such as its length-weight and conditions. This study aims to examine the relationship of length-weight and condition factors of four dominant fishes caught, which are ecologically and economically important, namely Lates calcarifer, Parachaetodon ocellatus, Stephanolepis cirrhifer, and Chelmon rostractus.These fish species were dominantly caught using bubu trap. The results of the length-weight relationship were b3 of all species, with an exponent value of 2.247 for the L. calcarifer; 1.932 for P. ocellatus; 2.74 for S. cirrhifer and 2.84 for C. rostractus. Fulton’s (K) condition factor for L. carifer ranged from 15.55-21.06 (average: 17.95), for P.ocellatus ranging from 27.30-35.59 (average: 30.38), for S. cirrhifer ranged from 17.58-27.23 (average: 21.29), and for C. rostractus ranging from 21.86-30.95 (average: 24.24). While the relative weight (Wr) condition factor average value of L. calcarifer is 102.53, P. ocellatus is 100.88, S. cirrhifer is 101.72, and C. rostractus is 101.11. It is concluded that the fish had an allometrict growth pattern, and condition factor values indicate good aquatic environmental conditions and a balance between prey and predatorKeywords: Length-Weight; Growth Pattern; Condition Factors","PeriodicalId":52680,"journal":{"name":"Aceh Journal of Animal Science","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44823530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Bangka Belitung Islands (Sumatra, Indonesia) has various coastal resources, e.g., coral reefs, seagrass beds, mangrove forests. However, the coral community has been threatened by anthropogenic activities, i.e., tin mining and illegal tin mining. Threatened species assessment is important for mitigation of coral losses and management. The ojective of the present study was to examine the status of Scleractinian corals in Bangka Belitung Islands, Indonesia. A line intercept transect was performed for the coral reef survey. Live and dead coral cover were recorded in the three locations. Corals species were identified following taxonomic revisions. The results showed that there were 142 species of Scleractinian corals recorded from Bangka Belitung Islands. Of these, 22 species are the new report from the areas of the the eastern part of Belitung Island. Family of Merulinidae, Acroporidae, and Poritidae were predominant group in this region. It is concluded that the condition of the coral reef ecosystem in the Belitung Islands is relatively good, but fair in Gaspar Strait and Bangka Island. Keywords: Coral cover, coral diversity, hard coral, Scleractinia
{"title":"Shallow-water hard corals (Hexacorallia: Scleractinia) from Bangka Belitung Islands Waters, Indonesia","authors":"Singgih Afifa Putra, H. Akbar, I. Syari","doi":"10.13170/ajas.4.2.14571","DOIUrl":"https://doi.org/10.13170/ajas.4.2.14571","url":null,"abstract":"Bangka Belitung Islands (Sumatra, Indonesia) has various coastal resources, e.g., coral reefs, seagrass beds, mangrove forests. However, the coral community has been threatened by anthropogenic activities, i.e., tin mining and illegal tin mining. Threatened species assessment is important for mitigation of coral losses and management. The ojective of the present study was to examine the status of Scleractinian corals in Bangka Belitung Islands, Indonesia. A line intercept transect was performed for the coral reef survey. Live and dead coral cover were recorded in the three locations. Corals species were identified following taxonomic revisions. The results showed that there were 142 species of Scleractinian corals recorded from Bangka Belitung Islands. Of these, 22 species are the new report from the areas of the the eastern part of Belitung Island. Family of Merulinidae, Acroporidae, and Poritidae were predominant group in this region. It is concluded that the condition of the coral reef ecosystem in the Belitung Islands is relatively good, but fair in Gaspar Strait and Bangka Island. Keywords: Coral cover, coral diversity, hard coral, Scleractinia","PeriodicalId":52680,"journal":{"name":"Aceh Journal of Animal Science","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46322945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
K. Marimuthu, Harysooria Palaniandya, Z. Muchlisin
Water quality parameters influence the growth and survival of different stages of fishes and hence determining the optimal water quality variables is greatly important for any aquaculture farming. Among the variables, water pH is considered the key factors and plays an important role in the maintenance of the homeostasis in fishes. A study was conducted to determine the influence of different water pH on the incubation period, hatching rate and survival rate of African catfish (Clarias gariepinus) larvae. The fertilized eggs were incubated at 28.0 ± 1.0 °C with different levels of water pH ranging from 3-10. Twenty four pH levels were tested for incubation period and egg hatchability while 15 pH levels were tested for larval survivability in a completely randomized design with three replicates for each treatment. Just hatched larvae were used for this study and the effect was observed until 72 h post-hatching. Water pH was maintained by the addition of NaOH or H2SO4solutions. The incubation times of fertilized eggs were recorded to be 23.5–25.0 h at pH levels of 6.1–8.8. The increased incubation time was noticed at the rest of the acidic and alkaline pH levels. Significantly highest hatching rate was observed at the pH levels of 6.7–7.6 compared to those at lower and higher pH levels (P0.05). However, no significant differences (P0.05) were recognized in the hatching rates at the pH levels of 6.7 – 7.6. No hatching occurred at low pH levels between 3.1 and 3.4 and at high pH of 10. After 72 h of exposure to different pH levels, no larvae survived at pH levels below 4.5 and above 9.0. Highest larval survival (98%) was observed at pH 7.0 followed by 94% at pH 7.5 and 92% at pH 6.5. The results obtained in the present study revealed embryos and larvae can survive and tolerate to a low pH level of 3.7 and 4.5, respectively but the survival rates decreased with decreasing pH levels. Therefore, a water pH level of 6.7–7.5 is highly recommended for optimal hatching and highest larval viability of C. gariepinus.
{"title":"Effect of different water pH on hatching and survival rates of African catfish Clarias gariepinus (Pisces: Clariidae)","authors":"K. Marimuthu, Harysooria Palaniandya, Z. Muchlisin","doi":"10.13170/AJAS.4.2.13574","DOIUrl":"https://doi.org/10.13170/AJAS.4.2.13574","url":null,"abstract":"Water quality parameters influence the growth and survival of different stages of fishes and hence determining the optimal water quality variables is greatly important for any aquaculture farming. Among the variables, water pH is considered the key factors and plays an important role in the maintenance of the homeostasis in fishes. A study was conducted to determine the influence of different water pH on the incubation period, hatching rate and survival rate of African catfish (Clarias gariepinus) larvae. The fertilized eggs were incubated at 28.0 ± 1.0 °C with different levels of water pH ranging from 3-10. Twenty four pH levels were tested for incubation period and egg hatchability while 15 pH levels were tested for larval survivability in a completely randomized design with three replicates for each treatment. Just hatched larvae were used for this study and the effect was observed until 72 h post-hatching. Water pH was maintained by the addition of NaOH or H2SO4solutions. The incubation times of fertilized eggs were recorded to be 23.5–25.0 h at pH levels of 6.1–8.8. The increased incubation time was noticed at the rest of the acidic and alkaline pH levels. Significantly highest hatching rate was observed at the pH levels of 6.7–7.6 compared to those at lower and higher pH levels (P0.05). However, no significant differences (P0.05) were recognized in the hatching rates at the pH levels of 6.7 – 7.6. No hatching occurred at low pH levels between 3.1 and 3.4 and at high pH of 10. After 72 h of exposure to different pH levels, no larvae survived at pH levels below 4.5 and above 9.0. Highest larval survival (98%) was observed at pH 7.0 followed by 94% at pH 7.5 and 92% at pH 6.5. The results obtained in the present study revealed embryos and larvae can survive and tolerate to a low pH level of 3.7 and 4.5, respectively but the survival rates decreased with decreasing pH levels. Therefore, a water pH level of 6.7–7.5 is highly recommended for optimal hatching and highest larval viability of C. gariepinus.","PeriodicalId":52680,"journal":{"name":"Aceh Journal of Animal Science","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47582611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
H. S. Rathore, Naveen Kumar, Kiranjeet Singh, S. K. Maiti, S. Shrivastava, S. Shivaramu, S. Saxena, Ravi K. Agarwal
The abdominal wall hernias resulting due to trauma or other clinical conditions are common in animals. Large hernias required the use of synthetic mesh, which is costly and may result in infection, fistula formation, and pain. Application of biomaterials in hernia repair causes a reduction in pain, reduced recovery time, and rate of recurrence. The study was undertaken to test the acellular bubaline diaphragm matrix (BDiaM) and acellular caprine rumen matrix (CRuM) for the repaired hernia in clinical cases. Fresh bubaline diaphragm and caprine rumen were decellularized using sodium deoxycholate (1% for CRuM and 2% for BDiaM) for 48h. Acellularity was ascertained histologically and by DNA quantification. Histologically, both the matrices showed complete acellularity and orderly arranged collagen fibers after 48 h. The DNA contents were significantly (P0.05) reduced in both the matrices in comparison to the native matrices. The BDiaM and CRuM matrices were applied in eight and nine clinical cases of abdominal wall defects, respectively. Animals with BDiaM and CRuM matrices recovered uneventfully and remained sound at least up to 3 months. Hematological and immunological findings were unremarkable. BDiaM and CRuM matrices showed good results without complications. Keywords:Biocompatibility, Bubaline diaphragm matrix, Caprine rumen matrix, DNA quantification, ELISA, SDS-PAGE
{"title":"Clinical application of acellular matrix derived from the bubaline diaphragm and caprine rumen for the repair of abdominal wall defects in animals","authors":"H. S. Rathore, Naveen Kumar, Kiranjeet Singh, S. K. Maiti, S. Shrivastava, S. Shivaramu, S. Saxena, Ravi K. Agarwal","doi":"10.13170/AJAS.4.2.13071","DOIUrl":"https://doi.org/10.13170/AJAS.4.2.13071","url":null,"abstract":"The abdominal wall hernias resulting due to trauma or other clinical conditions are common in animals. Large hernias required the use of synthetic mesh, which is costly and may result in infection, fistula formation, and pain. Application of biomaterials in hernia repair causes a reduction in pain, reduced recovery time, and rate of recurrence. The study was undertaken to test the acellular bubaline diaphragm matrix (BDiaM) and acellular caprine rumen matrix (CRuM) for the repaired hernia in clinical cases. Fresh bubaline diaphragm and caprine rumen were decellularized using sodium deoxycholate (1% for CRuM and 2% for BDiaM) for 48h. Acellularity was ascertained histologically and by DNA quantification. Histologically, both the matrices showed complete acellularity and orderly arranged collagen fibers after 48 h. The DNA contents were significantly (P0.05) reduced in both the matrices in comparison to the native matrices. The BDiaM and CRuM matrices were applied in eight and nine clinical cases of abdominal wall defects, respectively. Animals with BDiaM and CRuM matrices recovered uneventfully and remained sound at least up to 3 months. Hematological and immunological findings were unremarkable. BDiaM and CRuM matrices showed good results without complications. Keywords:Biocompatibility, Bubaline diaphragm matrix, Caprine rumen matrix, DNA quantification, ELISA, SDS-PAGE","PeriodicalId":52680,"journal":{"name":"Aceh Journal of Animal Science","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48638846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ine Karni, D. Handijatno, L. T. Suwanti, K. Kusnoto, J. Rahmahani, W. Tyasningsih
Almost all regions in Nusa Tenggara Timur East Nusa Tenggara (NTT) Province Indonesia are endemic areas of Haemorragic Septicaemia (HS), which is caused by Pasteurella multocida (P. multocida ) Serotypes B: 2. The fragment pfhaB1gene is one of the virulence factors of P. multocida.The objective of this study was to determaine the phylogenetic, homology of P. multocidapfhaB1gene fragment of isolatedfrom Buffalo and Cattle in NTT. The P. multocida isolateswere re-cultured and further microscopic examined the biochemical tests, PCR, sequencing, homology, and phylogenetic relatedness test. P. multocida was observed as gram negative, coccobacilus, no growth on MacConkey Agar, does not produce H2S and gas, nonmotile and indole positive, does not produce urease enzymes, does not use citrate as a carbon source, does not ferment maltose and lactose but it does ferment glucose, sucrose and mannitol. ThepfhaB1gene fragmentfrom buffalo and cattle NTT isolates and also Katha strain vaccine showed DNA band 506 bp. P.multocida isolates from buffalo and cattle in NTT have 91% - 99% score homology with the comparative isolate. The isolate P. multocida from buffalo and cattle in NTT are in one cluster and their phylogenetic relatedness is close to isolates from Iran and India. It is concluded that the pfhaB1gene fragmentof P. multocida from buffalo and cattle isolates have phylogenetic relatedness close and homolog with the other comparative isolates. Keywords: Haemorrhagic Septicaemia; Nusa Tenggara Timur; Pasteurella multocida; pfhaB1gene
{"title":"Molecular characterization of Pasteurella multocida pfhaB1 gene fragment from buffalo and cattle isolates from Nusa Tenggara Timur Indonesia","authors":"Ine Karni, D. Handijatno, L. T. Suwanti, K. Kusnoto, J. Rahmahani, W. Tyasningsih","doi":"10.13170/AJAS.4.2.13581","DOIUrl":"https://doi.org/10.13170/AJAS.4.2.13581","url":null,"abstract":"Almost all regions in Nusa Tenggara Timur East Nusa Tenggara (NTT) Province Indonesia are endemic areas of Haemorragic Septicaemia (HS), which is caused by Pasteurella multocida (P. multocida ) Serotypes B: 2. The fragment pfhaB1gene is one of the virulence factors of P. multocida.The objective of this study was to determaine the phylogenetic, homology of P. multocidapfhaB1gene fragment of isolatedfrom Buffalo and Cattle in NTT. The P. multocida isolateswere re-cultured and further microscopic examined the biochemical tests, PCR, sequencing, homology, and phylogenetic relatedness test. P. multocida was observed as gram negative, coccobacilus, no growth on MacConkey Agar, does not produce H2S and gas, nonmotile and indole positive, does not produce urease enzymes, does not use citrate as a carbon source, does not ferment maltose and lactose but it does ferment glucose, sucrose and mannitol. ThepfhaB1gene fragmentfrom buffalo and cattle NTT isolates and also Katha strain vaccine showed DNA band 506 bp. P.multocida isolates from buffalo and cattle in NTT have 91% - 99% score homology with the comparative isolate. The isolate P. multocida from buffalo and cattle in NTT are in one cluster and their phylogenetic relatedness is close to isolates from Iran and India. It is concluded that the pfhaB1gene fragmentof P. multocida from buffalo and cattle isolates have phylogenetic relatedness close and homolog with the other comparative isolates. Keywords: Haemorrhagic Septicaemia; Nusa Tenggara Timur; Pasteurella multocida; pfhaB1gene","PeriodicalId":52680,"journal":{"name":"Aceh Journal of Animal Science","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46943178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
of endoparasitic worms in skipjack tuna (Katsuwonus pelamis) in Sibolga Waters. Sampling conducted in Debora Private Fishing Port, Sibolga for 20 fishes that object weighing 740 – 1200 gr and length within 37,2 – 41,4 cm. The identification process is carried out in the laboratory using a microscope 40x and 100x total magnification. The endoparasitic worms found are Echinorhynchus sp. (100% intestinal and 10% stomach insidences, 8,6 intensities), Acanthocephalus sp. (25% Intestinal insidences, 1,6 intensities), Rhadinorhynchus sp. (25% intestinal and 5% stomach insidences, 1,5 intensities), Leptorhynchoides sp. (25% intestinal insidences, 1 intensity), Neoechinorhynchus sp. (25% intestinal insidences, 1,4 intensities), Pomphorhynchus sp. (10% intestinal insidences, 1,5 intensities), and Apororhynchus sp. (10% intestinal insidences, 1 intensity).
{"title":"Inventory of Gastrointestinal Endoparasitic Worms of Skipjack Tuna (Katsuwonus pelamis) in Sibolga Waters","authors":"E. Yusni, R. Uliya","doi":"10.13170/ajas.4.2.14129","DOIUrl":"https://doi.org/10.13170/ajas.4.2.14129","url":null,"abstract":"of endoparasitic worms in skipjack tuna (Katsuwonus pelamis) in Sibolga Waters. Sampling conducted in Debora Private Fishing Port, Sibolga for 20 fishes that object weighing 740 – 1200 gr and length within 37,2 – 41,4 cm. The identification process is carried out in the laboratory using a microscope 40x and 100x total magnification. The endoparasitic worms found are Echinorhynchus sp. (100% intestinal and 10% stomach insidences, 8,6 intensities), Acanthocephalus sp. (25% Intestinal insidences, 1,6 intensities), Rhadinorhynchus sp. (25% intestinal and 5% stomach insidences, 1,5 intensities), Leptorhynchoides sp. (25% intestinal insidences, 1 intensity), Neoechinorhynchus sp. (25% intestinal insidences, 1,4 intensities), Pomphorhynchus sp. (10% intestinal insidences, 1,5 intensities), and Apororhynchus sp. (10% intestinal insidences, 1 intensity).","PeriodicalId":52680,"journal":{"name":"Aceh Journal of Animal Science","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66278476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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