Pub Date : 2021-09-30DOI: 10.32345/2664-4738.3.2021.03
D. Maltsev
Relevance. The results of five meta-analyzes of randomized controlled clinical trials indicate an association between genetic deficiency of the folate cycle (GDFC) and autism spectrum disorders (ASD) in children. Autoimmune mechanisms play a special role in the pathogenesis of encephalopathy in children with ASD associated with GDFC. Objective: to study the structure of autoimmune reactions in children with ASD associated with GDFC, according to the accumulated evidence base and to identify associations of laboratory signs of autoimmunity and microorganisms to improve understanding of encephalopathy pathogenesis and diagnostic, monitoring and treatment algorithms. Materials and methods. The medical data of 225 children aged 2 to 9 years with GDFC, who had clinical manifestations of ASD (183 boys and 42 girls) were retrospectively analyzed. The diagnosis of ASD was made by child psychiatrists according to the criteria DSM-IV-TR (Diagnostic and Statistical Manual of mental disorders) and ICD-10 (The International Statistical Classification of Diseases and Related Health Problems) (study group; SG). The control group (CG) included 51 clinically healthy children (37 boys and 14 girls) of similar age and gender distribution who did not suffer from GDFC and ASD. Pathogenic polymorphic variants of folate cycle genes were determined by PCR with restriction (Sinevo, Ukraine). Autoantibodies to autoantigens of CNS subcortical ganglion neurons in blood serum were determined using a Cunningham panel (Moleculera Labs, Inc, USA). Serum autoantibodies to neurons of the mesolimbic system of the brain were identified by ELISA (MDI Limbach Berlin GmbH, Germany). Autoimmunization to myelin was assessed by serum autoantibody titer to basic myelin protein (ELISA) and signs of neutrophil and CD8+ T-lymphocyte sensitization to hemispheric white matter autoantigens (cell-based assay; department of neuroimmunology at the Neurosurgery Institute; Ukraine). Serum autoantibodies to nuclei of connective tissue cells and striated muscle proteins were determined by western blot analysis (Sinevo, Ukraine). To determine the significance of the differences between the indicators in the observation groups, we used the Student's parametric T-test with the confidence probability p and the nonparametric criterion – the number of signs Z according to Urbach Yu.V. The odds ratio (OR) and the 95% confidence interval (95% CI) were used to study the associations between the studied indicators. The study was performed as a fragment of research work commissioned by the Ministry of Health of Ukraine (№ state registration 0121U107940). Research. Positive results of the Cunningham panel occurred in 32%, laboratory signs of autoimmunization to neurons of the mesolimbic system – 36%, myelin of white matter of the hemispheres – 43%, nuclei autoantigens of connective tissue cells – 53%, proteins of striated muscles – 48% of cases among children SG (in general – 68% of cases; p < 0.05; Z < Z0.
关联随机对照临床试验的五项荟萃分析结果表明，儿童叶酸循环遗传缺陷（GDFC）与自闭症谱系障碍（ASD）之间存在关联。自身免疫机制在伴有GDFC的ASD患儿脑病的发病机制中起着特殊作用。目的：根据积累的证据基础，研究ASD合并GDFC的儿童自身免疫反应的结构，并确定自身免疫的实验室体征与微生物的相关性，以提高对脑病发病机制和诊断、监测和治疗算法的理解。材料和方法。回顾性分析225例2～9岁GDFC患儿（男183例，女42例）的ASD临床表现。ASD的诊断由儿童精神科医生根据标准DSM-IV-TR（精神障碍诊断和统计手册）和ICD-10（疾病和相关健康问题的国际统计分类）（研究组；SG）进行。对照组（CG）包括51名年龄和性别分布相似的临床健康儿童（37名男孩和14名女孩），他们没有患有GDFC和ASD。叶酸循环基因的致病性多态性变体通过限制性PCR测定（Sinevo，乌克兰）。使用Cunningham小组（Molecular Labs，Inc，USA）测定血清中CNS皮层下神经节神经元自身抗原的自身抗体。通过ELISA（MDI Limbach Berlin GmbH，德国）鉴定大脑中边缘系统神经元的血清自身抗体。通过对碱性髓鞘蛋白的血清自身抗体滴度（ELISA）和中性粒细胞和CD8+T淋巴细胞对半球白质自身抗原的敏化迹象来评估对髓鞘的自身免疫（基于细胞的测定；乌克兰神经外科研究所神经免疫学系）。通过蛋白质印迹分析（Sinevo，Ukraine）测定结缔组织细胞核和横纹肌蛋白的血清自身抗体。为了确定观察组中指标之间差异的显著性，我们使用了具有置信概率p的Student参数T检验和非参数标准——根据Urbach Yu的符号数Z。V.比值比（OR）和95%置信区间（95%CI）用于研究所研究指标之间的相关性。这项研究是乌克兰卫生部委托进行的研究工作的一部分(№ 州注册0121U107940）。研究Cunningham小组的阳性结果发生在32%，中边缘系统神经元自身免疫的实验室迹象发生在36%，大脑半球白质髓鞘发生在43%，结缔组织细胞的细胞核自身抗原发生在53%，横纹肌蛋白——48%的儿童SG病例（一般情况下——68%的病例；p<0.05；Z
{"title":"THE RESULTS OF THE SEARCH FOR LABORATORY SIGNS OF AUTOIMMUNE REACTIONS TO CEREBRAL AND EXTRACEREBRAL AUTOANTIGENS IN CHILDREN WITH AUTISM SPECTRUM DISORDERS ASSOCIATED WITH GENETIC DEFICIENCY OF THE FOLATE CYCLE","authors":"D. Maltsev","doi":"10.32345/2664-4738.3.2021.03","DOIUrl":"https://doi.org/10.32345/2664-4738.3.2021.03","url":null,"abstract":"Relevance. The results of five meta-analyzes of randomized controlled clinical trials indicate an association between genetic deficiency of the folate cycle (GDFC) and autism spectrum disorders (ASD) in children. Autoimmune mechanisms play a special role in the pathogenesis of encephalopathy in children with ASD associated with GDFC. \u0000Objective: to study the structure of autoimmune reactions in children with ASD associated with GDFC, according to the accumulated evidence base and to identify associations of laboratory signs of autoimmunity and microorganisms to improve understanding of encephalopathy pathogenesis and diagnostic, monitoring and treatment algorithms. \u0000Materials and methods. The medical data of 225 children aged 2 to 9 years with GDFC, who had clinical manifestations of ASD (183 boys and 42 girls) were retrospectively analyzed. The diagnosis of ASD was made by child psychiatrists according to the criteria DSM-IV-TR (Diagnostic and Statistical Manual of mental disorders) and ICD-10 (The International Statistical Classification of Diseases and Related Health Problems) (study group; SG). The control group (CG) included 51 clinically healthy children (37 boys and 14 girls) of similar age and gender distribution who did not suffer from GDFC and ASD. \u0000Pathogenic polymorphic variants of folate cycle genes were determined by PCR with restriction (Sinevo, Ukraine). Autoantibodies to autoantigens of CNS subcortical ganglion neurons in blood serum were determined using a Cunningham panel (Moleculera Labs, Inc, USA). Serum autoantibodies to neurons of the mesolimbic system of the brain were identified by ELISA (MDI Limbach Berlin GmbH, Germany). Autoimmunization to myelin was assessed by serum autoantibody titer to basic myelin protein (ELISA) and signs of neutrophil and CD8+ T-lymphocyte sensitization to hemispheric white matter autoantigens (cell-based assay; department of neuroimmunology at the Neurosurgery Institute; Ukraine). Serum autoantibodies to nuclei of connective tissue cells and striated muscle proteins were determined by western blot analysis (Sinevo, Ukraine). \u0000To determine the significance of the differences between the indicators in the observation groups, we used the Student's parametric T-test with the confidence probability p and the nonparametric criterion – the number of signs Z according to Urbach Yu.V. The odds ratio (OR) and the 95% confidence interval (95% CI) were used to study the associations between the studied indicators. \u0000The study was performed as a fragment of research work commissioned by the Ministry of Health of Ukraine (№ state registration 0121U107940). \u0000Research. Positive results of the Cunningham panel occurred in 32%, laboratory signs of autoimmunization to neurons of the mesolimbic system – 36%, myelin of white matter of the hemispheres – 43%, nuclei autoantigens of connective tissue cells – 53%, proteins of striated muscles – 48% of cases among children SG (in general – 68% of cases; p < 0.05; Z < Z0.","PeriodicalId":52737,"journal":{"name":"Medichna nauka Ukrayini","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42773328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-09-30DOI: 10.32345/2664-4738.3.2021.06
Y. Marushko, T. Hyshchak, Y. Todyka
Relevance. Upper respiratory tract infections (URTIs) are common in children and often progress with secondary complications such as otitis media, bronchitis, or pneumonia, especially in children with recurrent URTIs. Probiotics displayed immunomodulatory effects in children and adults, supporting immune functions to prevent winter diseases or common colds. Objectives. We assessed the effectiveness of a 6-week prophylaxis with probiotics (Lactobacillus helveticus Rosell®-52, Bifidobacterium infantis Rosell®-33 and Bifidobacterium bifidum Rosell®-71) for preventing primary infections and/or secondary complications in URTI-prone children. Methods. URTI-prone children were randomly divided into the probiotics (3×109 CFU/day; 6 weeks) or control arm (no preventive intervention). The number of URTIs, duration and related complications were monitored for 6 months. Resistance index, number of medical visits, and antibiotics prescriptions were also recorded. Results. After 2 months, probiotics reduced the number of URTIs (-2.34±0.13 vs -0.24±0.14; P<0.0001) and duration in (-1.13 ±0.18 vs -0.18±0.18 days; P=0.0011), and the number of secondary complications (-53% vs -5.8%; RR 0.5313 95% CI [0.3534, 0.7986] P= 0.0058). Probiotic lowered resistance index (P<0.0001), number of medical consultations with specialized physicians (P=0.0033) and antibiotics prescriptions (P<0.0001). Conclusions. Overall, a 6-week prophylaxis with combined probiotic (Lactobacillus helveticus Rosell®-52, Bifidobacterium infantis Rosell®-33 and Bifidobacterium bifidum Rosell®-71) in URTI-prone children exerted a significant and clinically important health benefit, decreasing the number and duration of URTI episodes and secondary complications and reducing the resistance index to a near-normal value. Furthermore, potential societal benefits of reducing health care use and inadequate antibiotic prescriptions in children favours the use of this probiotic product as a preventive strategy against URTIs and their consequences in frequently sick children. This trial was retrospectively registered on ClinicalTrials.gov (NCT04525040).
{"title":"PROPHYLACTIC EFFECT OF A PROBIOTIC INTERVENTION IN CHILDREN PRONE TO ACUTE UPPER RESPIRATORY TRACT INFECTIONS: A RANDOMIZED CONTROLLED TRIAL","authors":"Y. Marushko, T. Hyshchak, Y. Todyka","doi":"10.32345/2664-4738.3.2021.06","DOIUrl":"https://doi.org/10.32345/2664-4738.3.2021.06","url":null,"abstract":"Relevance. Upper respiratory tract infections (URTIs) are common in children and often progress with secondary complications such as otitis media, bronchitis, or pneumonia, especially in children with recurrent URTIs. Probiotics displayed immunomodulatory effects in children and adults, supporting immune functions to prevent winter diseases or common colds. \u0000Objectives. We assessed the effectiveness of a 6-week prophylaxis with probiotics (Lactobacillus helveticus Rosell®-52, Bifidobacterium infantis Rosell®-33 and Bifidobacterium bifidum Rosell®-71) for preventing primary infections and/or secondary complications in URTI-prone children. \u0000Methods. URTI-prone children were randomly divided into the probiotics (3×109 CFU/day; 6 weeks) or control arm (no preventive intervention). The number of URTIs, duration and related complications were monitored for 6 months. Resistance index, number of medical visits, and antibiotics prescriptions were also recorded. \u0000Results. After 2 months, probiotics reduced the number of URTIs (-2.34±0.13 vs -0.24±0.14; P<0.0001) and duration in (-1.13 ±0.18 vs -0.18±0.18 days; P=0.0011), and the number of secondary complications (-53% vs -5.8%; RR 0.5313 95% CI [0.3534, 0.7986] P= 0.0058). Probiotic lowered resistance index (P<0.0001), number of medical consultations with specialized physicians (P=0.0033) and antibiotics prescriptions (P<0.0001). \u0000Conclusions. Overall, a 6-week prophylaxis with combined probiotic (Lactobacillus helveticus Rosell®-52, Bifidobacterium infantis Rosell®-33 and Bifidobacterium bifidum Rosell®-71) in URTI-prone children exerted a significant and clinically important health benefit, decreasing the number and duration of URTI episodes and secondary complications and reducing the resistance index to a near-normal value. Furthermore, potential societal benefits of reducing health care use and inadequate antibiotic prescriptions in children favours the use of this probiotic product as a preventive strategy against URTIs and their consequences in frequently sick children. \u0000This trial was retrospectively registered on ClinicalTrials.gov (NCT04525040).","PeriodicalId":52737,"journal":{"name":"Medichna nauka Ukrayini","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47448513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-09-30DOI: 10.32345/2664-4738.3.2021.08
R. Chornopyshchuk, V. Nagaichuk, I. Gerashchenko, O. Nazarchuk, S.A. Sidorenkov, O. Chepliaka, L. Sidorenko
Relevance.One of the promising methods of treatment of patients with burns is the local use of sorption agents with antimicrobial properties. Objective: experimental study of antimicrobial properties and clinical evaluation of the effectiveness of local use of a new sorption composition based on nanosilica in the complex treatment of patients with burns. Materials and methods. The suggested sorption nanocomposition included highly dispersed silicon dioxide, polymethylsiloxane, decamethoxine, metronidazole. The experimental study involved the study of the antimicrobial properties of the sorption nanocomposition and antimicrobial agents which are part of it. The clinical material consisted of the results of examination of 42 patients with IIab-III degree burns with an area of 10-30% of the body surface. Depending on the tactics of local treatment, patients were divided into 2 groups: after early necrectomy, xenodermoplasty, the wound surface of the patients in the main group (n = 20) was treated with a solution of decamethoxine in combination with the same sorption powder. Treatment in the comparison group (n = 22) was similar except the use of sorption drugs. The examination included visual inspection of the injured area in combination with microbiological monitoring of the wound contents on the 3rd,7th,14th day. Results. The obtained results confirmed the sufficient antimicrobial potential of the studied sorption nanocomposition, the properties of which are not inferior to the existing antiseptics for museum and clinical strains of microorganisms and fungi. Signs of a more favorable wound healing process of the patients in the main group were observed: faster wound cleaning, less inflammatory reactions and much shorter preparation of wounds for grafting. Conclusions. The obtained results convincingly indicate the effectiveness of a multicomponent composite based on nanosilica with antimicrobial components in a comprehensive treatment of patients with burns.
{"title":"CLINICAL AND MICROBIOLOGICAL SUBSTANTIATION OF TOPICAL APPLICATION OF SORPTION HYDROPHILIC/HYDROPHOBIC COMPOSITION BASED ON NANOSILICA IN THE TREATMENT OF PATIENTS WITH BURNS","authors":"R. Chornopyshchuk, V. Nagaichuk, I. Gerashchenko, O. Nazarchuk, S.A. Sidorenkov, O. Chepliaka, L. Sidorenko","doi":"10.32345/2664-4738.3.2021.08","DOIUrl":"https://doi.org/10.32345/2664-4738.3.2021.08","url":null,"abstract":"Relevance.One of the promising methods of treatment of patients with burns is the local use of sorption agents with antimicrobial properties. \u0000Objective: experimental study of antimicrobial properties and clinical evaluation of the effectiveness of local use of a new sorption composition based on nanosilica in the complex treatment of patients with burns. \u0000Materials and methods. The suggested sorption nanocomposition included highly dispersed silicon dioxide, polymethylsiloxane, decamethoxine, metronidazole. \u0000The experimental study involved the study of the antimicrobial properties of the sorption nanocomposition and antimicrobial agents which are part of it. \u0000The clinical material consisted of the results of examination of 42 patients with IIab-III degree burns with an area of 10-30% of the body surface. Depending on the tactics of local treatment, patients were divided into 2 groups: after early necrectomy, xenodermoplasty, the wound surface of the patients in the main group (n = 20) was treated with a solution of decamethoxine in combination with the same sorption powder. Treatment in the comparison group (n = 22) was similar except the use of sorption drugs. The examination included visual inspection of the injured area in combination with microbiological monitoring of the wound contents on the 3rd,7th,14th day. \u0000Results. The obtained results confirmed the sufficient antimicrobial potential of the studied sorption nanocomposition, the properties of which are not inferior to the existing antiseptics for museum and clinical strains of microorganisms and fungi. \u0000Signs of a more favorable wound healing process of the patients in the main group were observed: faster wound cleaning, less inflammatory reactions and much shorter preparation of wounds for grafting. \u0000Conclusions. The obtained results convincingly indicate the effectiveness of a multicomponent composite based on nanosilica with antimicrobial components in a comprehensive treatment of patients with burns.","PeriodicalId":52737,"journal":{"name":"Medichna nauka Ukrayini","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48960565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-09-30DOI: 10.32345/2664-4738.3.2021.09
M. Korshun, Y. Martіianova
Relevance. The use of pesticides in the national economy is to destroy or inhibit the growth of harmful plants and to protect crops from pathogens requires a mandatory assessment of their environmental hygienic hazard. Objective of our study is to assess the hazards of three new pesticides for cereal grains protection: amicarbazone from the chemical class of triazolinone compounds, bicyclopyrone from the class of tricetones and pydiflumetofen from the class of carboxamides, in terms of ecotoxicity and environmental behavior. Materials and methods. Hazard assessment of amicarbazone, bicyclopyrone and pydiflumetofen was performed on the basis of data sources on their physico-chemical characteristics, toxicometry parameters for different species of living things and indicators of environmental behavior. For potential hazards integral assess for terrestrial ecosystems an ecotox was calculated taking into account mammalian toxicity, persistence and maximum rate consumption. To identify the limiting section of migration, the mathematical modeling was performed in the systems "soil-water", "soil-plants" and "soil-atmospheric air". Results. Amicarbazone has been shown to be extremely toxic to algae and highly toxic to higher aquatic plants (HAP) (hazard class I), moderately toxic (class IV) to mammals, mildly toxic (class III) to birds, soil mesofauna and invertebrates, virtually non-toxic to bees and fish; bicyclopyrone - extremely toxic to HAP (class I) and moderately toxic (class II) to the most sensitive algae, mildly toxic (class III) to birds, fish and invertebrates and virtually non-toxic to other terrestrial fauna; pydiflumetofen is highly toxic (class I) to fish and invertebrates, moderately toxic (class II) to algae and HAP, virtually non-toxic to all terrestrial biota. Pydiflumetofen has been shown to be a highly resistant and poorly mobile in soil; amicarbazone is stable and mobile; bicyclopyrone is highly resistant in laboratory experiments and moderately stable in field experiments, its mobility varies in a wide range: from very mobile to less mobile in some soils. The danger for terrestrial biocenoses of all studied pesticides under different soil and climatic conditions is lower by (1–5) orders of magnitude in comparison with DDT; the lowest is the ecotoxicity of bicyclopyrone, the highest – pydiflumetofen. All test substances are highly stable in water. The leading section of their migration in environment is the system "soil – water of ponds" and in case of pydiflumetofen – "soil – plants" as well.
{"title":"ECOLOGICAL AND HYGIENIC ASSESSMENT OF NEW PESTICIDES FOR GRAIN CEREALS CHEMICAL PROTECTION","authors":"M. Korshun, Y. Martіianova","doi":"10.32345/2664-4738.3.2021.09","DOIUrl":"https://doi.org/10.32345/2664-4738.3.2021.09","url":null,"abstract":"Relevance. The use of pesticides in the national economy is to destroy or inhibit the growth of harmful plants and to protect crops from pathogens requires a mandatory assessment of their environmental hygienic hazard. \u0000Objective of our study is to assess the hazards of three new pesticides for cereal grains protection: amicarbazone from the chemical class of triazolinone compounds, bicyclopyrone from the class of tricetones and pydiflumetofen from the class of carboxamides, in terms of ecotoxicity and environmental behavior. \u0000Materials and methods. Hazard assessment of amicarbazone, bicyclopyrone and pydiflumetofen was performed on the basis of data sources on their physico-chemical characteristics, toxicometry parameters for different species of living things and indicators of environmental behavior. For potential hazards integral assess for terrestrial ecosystems an ecotox was calculated taking into account mammalian toxicity, persistence and maximum rate consumption. To identify the limiting section of migration, the mathematical modeling was performed in the systems \"soil-water\", \"soil-plants\" and \"soil-atmospheric air\". \u0000Results. Amicarbazone has been shown to be extremely toxic to algae and highly toxic to higher aquatic plants (HAP) (hazard class I), moderately toxic (class IV) to mammals, mildly toxic (class III) to birds, soil mesofauna and invertebrates, virtually non-toxic to bees and fish; bicyclopyrone - extremely toxic to HAP (class I) and moderately toxic (class II) to the most sensitive algae, mildly toxic (class III) to birds, fish and invertebrates and virtually non-toxic to other terrestrial fauna; pydiflumetofen is highly toxic (class I) to fish and invertebrates, moderately toxic (class II) to algae and HAP, virtually non-toxic to all terrestrial biota. Pydiflumetofen has been shown to be a highly resistant and poorly mobile in soil; amicarbazone is stable and mobile; bicyclopyrone is highly resistant in laboratory experiments and moderately stable in field experiments, its mobility varies in a wide range: from very mobile to less mobile in some soils. The danger for terrestrial biocenoses of all studied pesticides under different soil and climatic conditions is lower by (1–5) orders of magnitude in comparison with DDT; the lowest is the ecotoxicity of bicyclopyrone, the highest – pydiflumetofen. All test substances are highly stable in water. The leading section of their migration in environment is the system \"soil – water of ponds\" and in case of pydiflumetofen – \"soil – plants\" as well.","PeriodicalId":52737,"journal":{"name":"Medichna nauka Ukrayini","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44108194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-09-30DOI: 10.32345/2664-4738.3.2021.10
I. Lastivka, V.V. Antsupva, A. Babintseva, M. Unhurian, I. Ushko
Relevance. Kabuki Makeup Syndrome (KS) is a rare monogenic genetic disease characterized by multiple malformations. The phenotype includes specific facial features, skeletal and dermatoglyphic abnormalities, mental retardation, short stature. Most cases are associated with de novo mutations in the KMT2D and KMD6A genes. However, in 25% of patients with KS, the genetic basis remains unknown, which indicates the genetic heterogeneity of the disease and encourages further accumulation of clinical experience in KS. The article summarizes current data on the molecular geneticі aspects of the development of Kabuki Makeup Syndrome and describes its own clinical case of Kabuki Makeup Syndrome Type I. Objective: to summarize the data on modern molecular-genetic aspects of the development of Kabuki makeup syndrome on the example of a clinical case. Materials and methods. Analysis of scientific publications in the international electronic scientometric database Scopus, PubMed by keywords. Search depth – 15 years (2007-2021). The clinical case of Kabuki Makeup Syndrome from our own practice. Clinical and genealogical, molecular-genetic, cytogenetic, instrumental research methods. Results. According to current data, the development of Kabuki Makeup Syndrome is due to mutations in the KMT2D (MLL2) gene, which belongs to the genes that control embryogenesis. KMT2D functions as a promoter of the expression of other genes and the KDM6A gene; encodes a large multidomain protein that interacts with the SET1/COMPASS complex. KDM6A is a cofactor physically associated with the KMT2D-COMPASS complex and exhibits demethylase activity in histone 3. Gene mutations KMT2D and KDM6A associated with KS lead to a lack of functioning of the corresponding enzyme, which leads to impaired methylation of histones and active genes in many organs and tissues of the body. Depending on the type of mutation in the KMT2D and KMD6A genes, there are two types of Kabuki Makeup Syndrome. KS type 1 with autosomal dominant type of inheritance due to pathogenic mutations in the KMT2D gene in a heterozygous state on chromosome 12q13.12. 70% of patients have KS1. Type 2 KS is an X-linked disease that develops as a result of a heterozygous pathogenic mutation in the KDM6 gene. In most cases, KS mutations are sporadic, but families with parent-to-child transmission have been described. In patients with phenotypic signs of KS pathogenic mutations are detected in 75% of cases. Pathogenic mutations in the KMT2D gene can be detected in mosaic form, and the carrier can pass this mutation on to offspring. Pathogenic mutations have not been described in phenotypically healthy people. Here is our own observation. The girl with a combined congenital heart defect and multiple stigmas of dysembryogenesis was born at 36 weeks with a weight of 2930, 49 cm long, on the Apgar scale 8/8 points from the third planned pregnancy in parents who already had an older healthy boy. In connection with multiple malforma
{"title":"MOLECULAR-GENETIC ASPECTS OF KABUKI MAKEUP SYNDROME. Review","authors":"I. Lastivka, V.V. Antsupva, A. Babintseva, M. Unhurian, I. Ushko","doi":"10.32345/2664-4738.3.2021.10","DOIUrl":"https://doi.org/10.32345/2664-4738.3.2021.10","url":null,"abstract":"Relevance. Kabuki Makeup Syndrome (KS) is a rare monogenic genetic disease characterized by multiple malformations. The phenotype includes specific facial features, skeletal and dermatoglyphic abnormalities, mental retardation, short stature. Most cases are associated with de novo mutations in the KMT2D and KMD6A genes. However, in 25% of patients with KS, the genetic basis remains unknown, which indicates the genetic heterogeneity of the disease and encourages further accumulation of clinical experience in KS. The article summarizes current data on the molecular geneticі aspects of the development of Kabuki Makeup Syndrome and describes its own clinical case of Kabuki Makeup Syndrome Type I. \u0000Objective: to summarize the data on modern molecular-genetic aspects of the development of Kabuki makeup syndrome on the example of a clinical case. \u0000Materials and methods. Analysis of scientific publications in the international electronic scientometric database Scopus, PubMed by keywords. Search depth – 15 years (2007-2021). The clinical case of Kabuki Makeup Syndrome from our own practice. Clinical and genealogical, molecular-genetic, cytogenetic, instrumental research methods. \u0000Results. According to current data, the development of Kabuki Makeup Syndrome is due to mutations in the KMT2D (MLL2) gene, which belongs to the genes that control embryogenesis. KMT2D functions as a promoter of the expression of other genes and the KDM6A gene; encodes a large multidomain protein that interacts with the SET1/COMPASS complex. KDM6A is a cofactor physically associated with the KMT2D-COMPASS complex and exhibits demethylase activity in histone 3. Gene mutations KMT2D and KDM6A associated with KS lead to a lack of functioning of the corresponding enzyme, which leads to impaired methylation of histones and active genes in many organs and tissues of the body. Depending on the type of mutation in the KMT2D and KMD6A genes, there are two types of Kabuki Makeup Syndrome. KS type 1 with autosomal dominant type of inheritance due to pathogenic mutations in the KMT2D gene in a heterozygous state on chromosome 12q13.12. 70% of patients have KS1. Type 2 KS is an X-linked disease that develops as a result of a heterozygous pathogenic mutation in the KDM6 gene. In most cases, KS mutations are sporadic, but families with parent-to-child transmission have been described. In patients with phenotypic signs of KS pathogenic mutations are detected in 75% of cases. Pathogenic mutations in the KMT2D gene can be detected in mosaic form, and the carrier can pass this mutation on to offspring. Pathogenic mutations have not been described in phenotypically healthy people. \u0000Here is our own observation. The girl with a combined congenital heart defect and multiple stigmas of dysembryogenesis was born at 36 weeks with a weight of 2930, 49 cm long, on the Apgar scale 8/8 points from the third planned pregnancy in parents who already had an older healthy boy. In connection with multiple malforma","PeriodicalId":52737,"journal":{"name":"Medichna nauka Ukrayini","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47808362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-09-30DOI: 10.32345/2664-4738.3.2021.05
Shweta Patil, Sangita Phatale, B. Malipatil
Relevance. Diabetes mellitus is a group of metabolic disorders wherein hyperglycemia occurs either due to lack of insulin secretion and/or reduced insulin sensitivity of the tissues. One of the commonest complications of diabetes is neuropathy and its severity will depend on how long and how high the hyperglycemia has been prevalent. Chronic hyperglycemia in diabetes affects the peripheral nerves and slows psychomotor responses in persons who do not have proper glycemic control. This decreased psychomotor response because improper glycemic control affects the reaction time in diabetics. Objective: to assess and compare the auditory and visual reaction time in group-1 and group-2 diabetics and to correlate it with glycosylated hemoglobin (HbA1c) levels in both the groups. Methods. The study was conducted on 60 subjects aged between 40-50 years after obtaining the permission of the ethical committee of our institution. The group consisted of 60 well-controlled diabetic patients (HbA1c < 7%) with a history of diabetes for 1-10 years attending the medical OPD of Basaveshwar teaching and general hospital, Gulbarga. Diabetics with HbA1c 5-6% were grouped as group-1 and those with HbA1c 6-7% as group 2. The visual reaction time for a green and red light and auditory reaction time for tone and click sound were measured by using reaction time apparatus 2x4, Anand agencies Pune. HbA1c was done by the micro-column method. Results. The visual and auditory reaction time of diabetics with HbA1c 5-6% was faster as compared to the diabetics with HbA1c 6-7% with P< 0.001 and a statistically significant positive correlation was found between HbA1c levels and the visual and auditory reaction time. Conclusion. Poor glycemic control slows psychomotor responses and thus affects the visual and auditory reaction time.
{"title":"CORRELATION OF GLYCOSYLATED HEMOGLOBIN LEVELS WITH AUDITORY AND VISUAL REACTION TIME IN DIABETICS","authors":"Shweta Patil, Sangita Phatale, B. Malipatil","doi":"10.32345/2664-4738.3.2021.05","DOIUrl":"https://doi.org/10.32345/2664-4738.3.2021.05","url":null,"abstract":"Relevance. Diabetes mellitus is a group of metabolic disorders wherein hyperglycemia occurs either due to lack of insulin secretion and/or reduced insulin sensitivity of the tissues. One of the commonest complications of diabetes is neuropathy and its severity will depend on how long and how high the hyperglycemia has been prevalent. Chronic hyperglycemia in diabetes affects the peripheral nerves and slows psychomotor responses in persons who do not have proper glycemic control. This decreased psychomotor response because improper glycemic control affects the reaction time in diabetics. \u0000Objective: to assess and compare the auditory and visual reaction time in group-1 and group-2 diabetics and to correlate it with glycosylated hemoglobin (HbA1c) levels in both the groups. \u0000Methods. The study was conducted on 60 subjects aged between 40-50 years after obtaining the permission of the ethical committee of our institution. The group consisted of 60 well-controlled diabetic patients (HbA1c < 7%) with a history of diabetes for 1-10 years attending the medical OPD of Basaveshwar teaching and general hospital, Gulbarga. Diabetics with HbA1c 5-6% were grouped as group-1 and those with HbA1c 6-7% as group 2. The visual reaction time for a green and red light and auditory reaction time for tone and click sound were measured by using reaction time apparatus 2x4, Anand agencies Pune. HbA1c was done by the micro-column method. \u0000Results. The visual and auditory reaction time of diabetics with HbA1c 5-6% was faster as compared to the diabetics with HbA1c 6-7% with P< 0.001 and a statistically significant positive correlation was found between HbA1c levels and the visual and auditory reaction time. \u0000Conclusion. Poor glycemic control slows psychomotor responses and thus affects the visual and auditory reaction time.","PeriodicalId":52737,"journal":{"name":"Medichna nauka Ukrayini","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44998575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-09-30DOI: 10.32345/2664-4738.3.2021.07
I. Tepla
Relevance. Birth weight is one of the main indicators of intrauterine fetal development. The condition of the placenta reflects the influence of the maternal environment on the fetus. It is important to find out which placental growth parameters significantly affect the mass of dichorionic diamniotic (DHDA) twins. Objective: to investigate the relationship between indicators of placental growth and birth weight of dichorionic diamniotic twins. Materials and methods. One hundred thirty-five DCDA twin pairs were studied during 2016-2020: 68 pairs were opposite-sex, 32 – same-sex females and 35 – same-sex males. The gestational age ranged from 29 to 39.5 weeks, averaging 36,4 ± V1,74 weeks. The influence of such parameters as maximum and minimum placental diameters, as well as perimeter, area, circularity (Circ), roundness, thickness, mass (PM), volume (V), displacement of the umbilical cord insertion site from the placental disc centre along its maximum (XCD) and the minimum axis (YCD). Results. A strong positive correlation was found between PM and birth weight in the whole cohort of DCDA twins (r = 0.7059, p < 0.0001). It was strongest in the same-sex female group (r = 0.7886, p < 0.0001), then in the opposite-sex pairs (r = 0.7093, p < 0.0001), slightly weaker – in the same-sex male couples (r = 0.6065, p < 0.0001). The birth weight and PM correlated with almost all indicators that characterize the placental shape. The strongest correlation was with V (rV_BW = 0.66, p < 0.0001; rV_PM = 0.59, p < 0.0001) and the area*Circ (rS*Circ_BW = 0.64; p < 0.0001; rS*Circ_PM = 0.62; p < 0.0001). The multiple regression analysis of the effect of different morphometric parameters of the placentas on the birth weight has demonstrated the statistical significance of the following parameters: XCD, PM, V, YCD and Circ. The general regression model for the whole DCDA group: birth weight = 2242,3 + 0,8*МП + V – 10,0* XCD (R2 = 0,7002). Similar equations were calculated for different types of DCDA tweens. Thus, morphometric measures account for the 67-85% variances of the birth weight of the DCDA tweens. Conclusion. In the DCDA pairs, placental mass strongly positively correlated with birth weight. Both indicators are in strong relationship with V and their area*Circ. The closer the placental shape is to the circle, the higher its functional ability. Deterioration of the latter may be due to the far fixation of the umbilical cord from the center of the placental disc. The displacement along the maximum axis has a stronger impact comparing to the displacement relative to the minimum axis. The multiple regression analysis has revealed that statistical significance had XCD and PM or V in the whole types of twins. Proposed models demonstrate that 67-85% of the variability of birth weight may be accounted for by the three or four simple placental measures
{"title":"THE RELATIONSHIP BETWEEN PLACENTAL GROWTH RATES AND THE BIRTH WEIGHT IN DICHORIONIC DIAMNIOTIC TWINS","authors":"I. Tepla","doi":"10.32345/2664-4738.3.2021.07","DOIUrl":"https://doi.org/10.32345/2664-4738.3.2021.07","url":null,"abstract":"Relevance. Birth weight is one of the main indicators of intrauterine fetal development. The condition of the placenta reflects the influence of the maternal environment on the fetus. It is important to find out which placental growth parameters significantly affect the mass of dichorionic diamniotic (DHDA) twins. \u0000Objective: to investigate the relationship between indicators of placental growth and birth weight of dichorionic diamniotic twins. \u0000Materials and methods. One hundred thirty-five DCDA twin pairs were studied during 2016-2020: 68 pairs were opposite-sex, 32 – same-sex females and 35 – same-sex males. The gestational age ranged from 29 to 39.5 weeks, averaging 36,4 ± V1,74 weeks. The influence of such parameters as maximum and minimum placental diameters, as well as perimeter, area, circularity (Circ), roundness, thickness, mass (PM), volume (V), displacement of the umbilical cord insertion site from the placental disc centre along its maximum (XCD) and the minimum axis (YCD). \u0000Results. A strong positive correlation was found between PM and birth weight in the whole cohort of DCDA twins (r = 0.7059, p < 0.0001). It was strongest in the same-sex female group (r = 0.7886, p < 0.0001), then in the opposite-sex pairs (r = 0.7093, p < 0.0001), slightly weaker – in the same-sex male couples (r = 0.6065, p < 0.0001). The birth weight and PM correlated with almost all indicators that characterize the placental shape. The strongest correlation was with V (rV_BW = 0.66, p < 0.0001; rV_PM = 0.59, p < 0.0001) and the area*Circ (rS*Circ_BW = 0.64; p < 0.0001; rS*Circ_PM = 0.62; p < 0.0001). The multiple regression analysis of the effect of different morphometric parameters of the placentas on the birth weight has demonstrated the statistical significance of the following parameters: XCD, PM, V, YCD and Circ. The general regression model for the whole DCDA group: birth weight = 2242,3 + 0,8*МП + V – 10,0* XCD (R2 = 0,7002). Similar equations were calculated for different types of DCDA tweens. Thus, morphometric measures account for the 67-85% variances of the birth weight of the DCDA tweens. \u0000Conclusion. In the DCDA pairs, placental mass strongly positively correlated with birth weight. Both indicators are in strong relationship with V and their area*Circ. The closer the placental shape is to the circle, the higher its functional ability. Deterioration of the latter may be due to the far fixation of the umbilical cord from the center of the placental disc. The displacement along the maximum axis has a stronger impact comparing to the displacement relative to the minimum axis. The multiple regression analysis has revealed that statistical significance had XCD and PM or V in the whole types of twins. Proposed models demonstrate that 67-85% of the variability of birth weight may be accounted for by the three or four simple placental measures ","PeriodicalId":52737,"journal":{"name":"Medichna nauka Ukrayini","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46756999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-30DOI: 10.32345/2664-4738.2.2021.09
O. Iaremenko
Relevance. Achieving remission is one of the main goals in the treatment of patients with rheumatoid arthritis (RA). One of the determining predictors of the disease, according to the literature, is the serological variant of RA. However, there are conflicting data in scientific publications on the relationship between the presence of antibodies to cyclic citrullinated peptide (ACCP) and / or rheumatoid factor (RF) and the frequency and rate of remission. There is no unanimous opinion in the literature on the influence of the titer of serological markers of RA (ACCP and RF) on the possibility of achieving remission on the background of basic treatment, which prompted to conduct their own research to study this issue. Objective: to study the relationship between the presence / absence of serological markers of RA (ACCP, RF) and the frequency and timing of clinical and radiological remission of RA under the influence of treatment with traditional synthetic basic drugs and to analyze the relationship between ACCP and RF titers and the possibility of remission.Material and methods. The study analyzed the influence of serological status of patients with RA on the possibility and time of remission while taking the main non-biological basic drugs. The relationship between the presence and level of ACCP and / or RF and clinical and radiological remission in RA has been studied. The study included 128 patients. Analysis of RA activity and assessment of remission were performed after 6, 12 and 24 months of treatment, using the DAS28 activity scale and the dynamics of radiological changes on the Sharpe-van der Heide scale. Results. During the 2-year follow-up, clinical remission was observed three times more often in the group of patients negative for ACCP (anti-cyclic citrullinated peptide) (36.1% in the group ACCP-RF- compared with 12.5% in the group ACCP + RF ( +, χ2 = 7.74, p < 0.05, and in 33.3% in the group ACCP-RF +, a significant difference compared with ACCP + RF +, χ2 = 4.55, p <0.05). Early remission (during the first 6 months of treatment) was also more common in the group of patients with no ACCP (χ2 = 10.7, p <0.01 and χ2 = 6.69, p <0.05, respectively). The rate of remission (the share of early in the structure of the total) in the four analyzed groups did not differ significantly and was 75%, 66.6%, 66.6% and 84.6%, respectively. The titer of ACCP in the group of patients who achieved remission was 240.8 ± 38.5 and did not differ significantly from that in the group of patients whose RA (rheumatoid arthritis) activity exceeded the remission threshold (187.8 ± 13.7, p> 0.05). There was also no significant difference between these two groups in the titers of the RF (rheumatoid factor): 257.9 ± 233.8 and 293.2 ± 257.3, respectively. Radiological remission was achieved in 46.7% of ACCP-negative patients and only in 10.6% of ACCP-positive patients (p <0.01). The absence of RF in the blood was also associated with a more frequent achievement of
的相关性。实现缓解是治疗类风湿性关节炎(RA)患者的主要目标之一。根据文献，该疾病的决定性预测因素之一是类风湿关节炎的血清学变异。然而，关于环瓜氨酸肽(ACCP)和/或类风湿因子(RF)抗体的存在与缓解频率和率之间的关系，科学出版物中存在相互矛盾的数据。在基础治疗的背景下，RA的血清学标志物(ACCP和RF)的滴度对缓解可能性的影响，文献中没有一致的看法，这促使他们进行了自己的研究来研究这一问题。目的:研究在传统合成基础药物治疗影响下，RA血清学标志物(ACCP、RF)的有无与RA临床和放射学缓解的频率和时间的关系，分析ACCP和RF滴度与缓解可能性的关系。材料和方法。本研究分析类风湿关节炎患者在服用主要非生物基础药物时，血清学状况对缓解可能性和时间的影响。ACCP和/或RF的存在和水平与RA的临床和放射学缓解之间的关系已被研究。该研究包括128名患者。治疗6个月、12个月和24个月后，采用DAS28活动度量表和Sharpe-van der Heide量表放射学动态变化分析RA活动度并评估缓解情况。结果。在2年随访期间，ACCP(抗环瓜氨酸肽)阴性组的临床缓解率为36.1%，而ACCP + RF组为12.5% (χ2 = 7.74, p < 0.05)， ACCP-RF +组为33.3%，与ACCP + RF +组比较差异有统计学意义(χ2 = 4.55, p 0.05)。两组患者类风湿因子(RF)滴度分别为257.9±233.8和293.2±257.3，差异无统计学意义。46.7%的accp阴性患者达到放射学缓解，而只有10.6%的accp阳性患者达到放射学缓解(p <0.01)。与RF阳性患者(15.4%，p <0.05)相比，血液中RF缺失也与更频繁的放射学缓解相关(34.2%的患者)。结论。发现临床缓解的频率，包括早期(治疗的前6个月)，是RA患者的3倍，ACCP阴性。临床缓解率(一般结构早期的比率)不取决于疾病的血清学变异:在所有分析组中，约三分之二的患者在基础治疗的前半期达到缓解。发病时RA的主要血清学标志物(ACCP和RF)滴度不影响临床和放射学缓解的可能性。血清阴性(ACCP或RF)患者的放射学缓解率是阴性患者的三倍。双重血清阳性对随后的关节破坏具有累加性影响。
{"title":"ASSOCIATION OF SEROLOGICAL STATUS WITH THE FREQUENCY OF CLINICAL AND RADIOLOGICAL REMISSION IN RHEUMATOID ARTHRITIS","authors":"O. Iaremenko","doi":"10.32345/2664-4738.2.2021.09","DOIUrl":"https://doi.org/10.32345/2664-4738.2.2021.09","url":null,"abstract":"Relevance. Achieving remission is one of the main goals in the treatment of patients with rheumatoid arthritis (RA). One of the determining predictors of the disease, according to the literature, is the serological variant of RA. However, there are conflicting data in scientific publications on the relationship between the presence of antibodies to cyclic citrullinated peptide (ACCP) and / or rheumatoid factor (RF) and the frequency and rate of remission. There is no unanimous opinion in the literature on the influence of the titer of serological markers of RA (ACCP and RF) on the possibility of achieving remission on the background of basic treatment, which prompted to conduct their own research to study this issue. Objective: to study the relationship between the presence / absence of serological markers of RA (ACCP, RF) and the frequency and timing of clinical and radiological remission of RA under the influence of treatment with traditional synthetic basic drugs and to analyze the relationship between ACCP and RF titers and the possibility of remission.Material and methods. The study analyzed the influence of serological status of patients with RA on the possibility and time of remission while taking the main non-biological basic drugs. The relationship between the presence and level of ACCP and / or RF and clinical and radiological remission in RA has been studied. The study included 128 patients. Analysis of RA activity and assessment of remission were performed after 6, 12 and 24 months of treatment, using the DAS28 activity scale and the dynamics of radiological changes on the Sharpe-van der Heide scale. \u0000Results. During the 2-year follow-up, clinical remission was observed three times more often in the group of patients negative for ACCP (anti-cyclic citrullinated peptide) (36.1% in the group ACCP-RF- compared with 12.5% in the group ACCP + RF ( +, χ2 = 7.74, p < 0.05, and in 33.3% in the group ACCP-RF +, a significant difference compared with ACCP + RF +, χ2 = 4.55, p <0.05). Early remission (during the first 6 months of treatment) was also more common in the group of patients with no ACCP (χ2 = 10.7, p <0.01 and χ2 = 6.69, p <0.05, respectively). The rate of remission (the share of early in the structure of the total) in the four analyzed groups did not differ significantly and was 75%, 66.6%, 66.6% and 84.6%, respectively. The titer of ACCP in the group of patients who achieved remission was 240.8 ± 38.5 and did not differ significantly from that in the group of patients whose RA (rheumatoid arthritis) activity exceeded the remission threshold (187.8 ± 13.7, p> 0.05). There was also no significant difference between these two groups in the titers of the RF (rheumatoid factor): 257.9 ± 233.8 and 293.2 ± 257.3, respectively. Radiological remission was achieved in 46.7% of ACCP-negative patients and only in 10.6% of ACCP-positive patients (p <0.01). The absence of RF in the blood was also associated with a more frequent achievement of ","PeriodicalId":52737,"journal":{"name":"Medichna nauka Ukrayini","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45035951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-30DOI: 10.32345/2664-4738.2.2021.01
I. Dzevulska, R. Matkivska, A.M. Sinitska, L.V. Prysiazhnjuk, A. Yanchyshyn
Relevance. Thermal burns of the skin cause the development of burns, the main factor of which is endogenous intoxication. The infusion of detoxification solutions is a mandatory component of the treatment of burns, as it corrects its course and prevents the development of certain stages and complications. According to the stage of the burn disease and the different direction of its links in its pathogenesis, infusion therapy should not only restore water-electrolyte balance and detoxify the body, but also contribute to the normalization and stabilization of vital (including immune) body functions. Objective: study of changes in the vessels of the hemo- and lymphomicrocirculatory system of Peyer's patches in burnt rats at the stages of burn disease, under conditions of intravenous infusion of isotonic sodium chloride solution and combined colloidal-hyperosmolar solutions (lactoprotein and sorbitolum). Materials and methods. The experiment was performed on 72 white rats. Experimental animals were divided into 4 groups (18 animals in each group). I, II, III - rats with skin burn trauma, which received a separate infusion of isotonic sodium chloride (I), lactoprotein with sorbitol (II) and hecoton (III), at a dose of 10 ml / kg. IV - intact animals (control group). Simulation of burn disease was performed by inflicting burn injury by applying to the lateral surfaces of the body of animals four copper plates, which were previously kept for 6 minutes in water with a constant temperature of 100 ° C. Histological and electron microscopic examination were performed. Light microscopy was used. Results. Intravenous administration of the applied infusion solutions caused various phase changes of the hemo- and lymphomicrocirculatory tract of Peyer's patches of the ileum of burnt rats, depending on the applied solution. In the case of infusion of burnt rats with 0.9% sodium chloride solution, the negative effects of burn disease were exacerbated, which were manifested by damage to the vascular wall of the hemo- and lymphocapillary channels, resulting in plasma and lymph seepage through the affected areas into the perivascular space. This process was accompanied by a violation of the rheological properties of blood, the formation of edema. The established "membrane-plastic effect" of lactoprotein with sorbitol revealed clear organ-specific features, which had manifestations of penetration through the damaged wall of microvascular electron-dense impregnations, which contributed to a significant thickening of the basement membrane in the wall of the blood capillary formation and its gradual formation of rounded membranous structure. This structure of variable electron density not only strengthened the vascular wall, but also served as a closure, helping to close the vascular lumen at the site of largest damage. The difference between the perinodal lymphatic capillaries in burnt rats infused with lactoprotein and sorbitol is characterized by the phenomenon of
的相关性。皮肤热烧伤引起烧伤的发展，其主要因素是内源性中毒。输注解毒溶液是治疗烧伤的强制性组成部分，因为它纠正其过程，防止某些阶段和并发症的发展。根据烧伤的不同阶段及其发病过程中各环节的不同方向，输液治疗不仅要恢复机体的水电解质平衡和解毒，而且要有助于机体重要功能(包括免疫功能)的正常化和稳定。目的:研究烧伤大鼠在静脉滴注等渗氯化钠溶液和复合胶体-高渗溶液(乳蛋白和山梨糖醇)条件下，Peyer’s patches的血液和淋巴微循环系统血管的变化。材料和方法。实验在72只大鼠身上进行。实验动物分为4组，每组18只。I、II、III:皮肤烧伤大鼠，分别输注等渗氯化钠(I)、山梨醇乳蛋白(II)和六烯酮(III)，剂量为10 ml / kg。IV -完整动物(对照组)。模拟烧伤，将4块铜板置于动物身体外侧表面，在100℃恒温水中保存6分钟，进行组织学和电镜检查。采用光镜观察。结果。所应用的输液溶液静脉给药后，烧伤大鼠回肠Peyer’s斑块的血液和淋巴微循环道根据所应用的溶液的不同发生了不同的相变化。在烧伤大鼠输注0.9%氯化钠溶液的情况下，烧伤疾病的负面影响加剧，表现为血液和淋巴毛细血管壁的损伤，导致血浆和淋巴液从受累部位渗漏到血管周围空间。这一过程伴随着血液流变特性的破坏，形成水肿。乳蛋白与山梨醇所建立的“膜塑效应”显示出明确的器官特异性，具有穿透微血管电子致密浸渍损伤壁的表现，导致毛细血管壁基底膜明显增厚，逐渐形成圆形膜状结构。这种变电子密度的结构不仅加强了血管壁，而且起到了封闭的作用，有助于在损伤最大的部位关闭血管腔。乳蛋白和山梨醇灌注烧伤大鼠淋巴结周围淋巴毛细血管的差异表现为淋巴毛细血管通道“溢出”，细胞处于不同的功能状态。在输注heecoton溶液的情况下，未观察到淋巴毛细血管通道“溢出”的影响，这表明免疫活性细胞的免疫功能得到了充分的发挥。结论。静脉输注胶体高渗溶液可引起佩耶氏贴片的血液和淋巴微循环道的各种变化。0.9%氯化钠溶液的输注导致烧伤疾病的负面后果加深，其表现形式是对血液和淋巴毛细血管壁的损害。乳蛋白与山梨醇和六糖醇溶液的输注有助于保存结节性淋巴毛细血管的血管壁。将含有山梨糖醇的乳蛋白溶液涂抹在受损的毛细血管和小静脉周围，形成环状膜，促进结构完好的淋巴细胞选择性再循环。
{"title":"COMPARATIVE CHARACTERISTICS OF CHANGES IN HEMO - AND LYMPHOMICROCIRCULATORY VESSELS OF ILEUM ACCUMULATED LYMPHOID NODES OF RATS IN THE CASE OF APPLICATION OF INFUSION SOLUTIONS IN EXPERIMENTAL BURN DISEASE","authors":"I. Dzevulska, R. Matkivska, A.M. Sinitska, L.V. Prysiazhnjuk, A. Yanchyshyn","doi":"10.32345/2664-4738.2.2021.01","DOIUrl":"https://doi.org/10.32345/2664-4738.2.2021.01","url":null,"abstract":"Relevance. Thermal burns of the skin cause the development of burns, the main factor of which is endogenous intoxication. The infusion of detoxification solutions is a mandatory component of the treatment of burns, as it corrects its course and prevents the development of certain stages and complications. According to the stage of the burn disease and the different direction of its links in its pathogenesis, infusion therapy should not only restore water-electrolyte balance and detoxify the body, but also contribute to the normalization and stabilization of vital (including immune) body functions. \u0000Objective: study of changes in the vessels of the hemo- and lymphomicrocirculatory system of Peyer's patches in burnt rats at the stages of burn disease, under conditions of intravenous infusion of isotonic sodium chloride solution and combined colloidal-hyperosmolar solutions (lactoprotein and sorbitolum). \u0000Materials and methods. The experiment was performed on 72 white rats. Experimental animals were divided into 4 groups (18 animals in each group). I, II, III - rats with skin burn trauma, which received a separate infusion of isotonic sodium chloride (I), lactoprotein with sorbitol (II) and hecoton (III), at a dose of 10 ml / kg. IV - intact animals (control group). \u0000Simulation of burn disease was performed by inflicting burn injury by applying to the lateral surfaces of the body of animals four copper plates, which were previously kept for 6 minutes in water with a constant temperature of 100 ° C. Histological and electron microscopic examination were performed. Light microscopy was used. \u0000Results. Intravenous administration of the applied infusion solutions caused various phase changes of the hemo- and lymphomicrocirculatory tract of Peyer's patches of the ileum of burnt rats, depending on the applied solution. In the case of infusion of burnt rats with 0.9% sodium chloride solution, the negative effects of burn disease were exacerbated, which were manifested by damage to the vascular wall of the hemo- and lymphocapillary channels, resulting in plasma and lymph seepage through the affected areas into the perivascular space. This process was accompanied by a violation of the rheological properties of blood, the formation of edema. The established \"membrane-plastic effect\" of lactoprotein with sorbitol revealed clear organ-specific features, which had manifestations of penetration through the damaged wall of microvascular electron-dense impregnations, which contributed to a significant thickening of the basement membrane in the wall of the blood capillary formation and its gradual formation of rounded membranous structure. This structure of variable electron density not only strengthened the vascular wall, but also served as a closure, helping to close the vascular lumen at the site of largest damage. The difference between the perinodal lymphatic capillaries in burnt rats infused with lactoprotein and sorbitol is characterized by the phenomenon of ","PeriodicalId":52737,"journal":{"name":"Medichna nauka Ukrayini","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47500026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-30DOI: 10.32345/2664-4738.2.2021.05
L. Kozak
Relevance. It was shown a wide therapeutic effects of intermittent hypoxic training (IHT) on the human organism which can increase the organism's resistance to damaging factors by inducing mechanisms of the safety use of energy resources. The objective: to investigate the protective effect of IHT applied at the beginning of the 30-day alcohol intoxication of rats on the redox processes and the state of the antioxidant system of blood and liver tissues. Materials and methods. The study was performed on white male rats, which were divided into four groups: the first - intact (control); animals of the second group received a 15% ethanol solution for 30 days as the only source of drink; third - animals under IHT; fourth - rats that IHT was applied at the beginning of 30 days alcohol intoxication. The amount of the consumed ethanol was monitored during the experiment. Intermittent hypoxic training was performed in the altitude chamber in the following mode: five 10-min “elevations” to a height of 6 km at a rate of 20 m/sec, intervals between the elevations being 30 min. The activity of antioxidant enzymes (superoxide dismutase, catalase, glutathione peroxidase), total antioxidant activity (IAOA), as well as the content of lactic and pyruvic acids, malone dialdehyde and diene conjugates were determined. The research materials were homogenates of liver tissues and blood. The process is two-phase, which obviously reflects the stages of alcoholism. Results. It was found that alcohol intoxication of animals adapted to IHT is accompanied by an increase in the activity of superoxide dismutase and glutathione peroxidase in the blood and liver tissue against rats that consumed ethanol as the only source of drink. A tendency to increase IAOA and catalase activity against rats that received ethanol for a long time under these conditions was revealed. Analyzing the process of consumption of ethanol solution by animals, we can conclude that animals have a pronounced craving for alcohol in this model of alcoholization. The application of IHT at the beginning of alcohol intoxication as a protective agent delays the phase of formation of a pronounced craving for ethanol and reduces the amount of daily alcohol consumption. Conclusions. Thus, sessions of short-term hypoxic effects at the beginning of chronic ethanol consumption, provide an increase in the antioxidant defence of these animals relative to rats with alcohol intoxication.
{"title":"PECULIARITIES OF ETHANOL INTOXICATION OF ANIMALS AT APPLICATION OF INTERMITTENT HYPOXIC TRAINING AT THE BEGINNING OF ALCOHOL CONSUMPTION","authors":"L. Kozak","doi":"10.32345/2664-4738.2.2021.05","DOIUrl":"https://doi.org/10.32345/2664-4738.2.2021.05","url":null,"abstract":"Relevance. It was shown a wide therapeutic effects of intermittent hypoxic training (IHT) on the human organism which can increase the organism's resistance to damaging factors by inducing mechanisms of the safety use of energy resources. The objective: to investigate the protective effect of IHT applied at the beginning of the 30-day alcohol intoxication of rats on the redox processes and the state of the antioxidant system of blood and liver tissues. Materials and methods. The study was performed on white male rats, which were divided into four groups: the first - intact (control); animals of the second group received a 15% ethanol solution for 30 days as the only source of drink; third - animals under IHT; fourth - rats that IHT was applied at the beginning of 30 days alcohol intoxication. The amount of the consumed ethanol was monitored during the experiment. Intermittent hypoxic training was performed in the altitude chamber in the following mode: five 10-min “elevations” to a height of 6 km at a rate of 20 m/sec, intervals between the elevations being 30 min. The activity of antioxidant enzymes (superoxide dismutase, catalase, glutathione peroxidase), total antioxidant activity (IAOA), as well as the content of lactic and pyruvic acids, malone dialdehyde and diene conjugates were determined. The research materials were homogenates of liver tissues and blood. The process is two-phase, which obviously reflects the stages of alcoholism. Results. It was found that alcohol intoxication of animals adapted to IHT is accompanied by an increase in the activity of superoxide dismutase and glutathione peroxidase in the blood and liver tissue against rats that consumed ethanol as the only source of drink. A tendency to increase IAOA and catalase activity against rats that received ethanol for a long time under these conditions was revealed. Analyzing the process of consumption of ethanol solution by animals, we can conclude that animals have a pronounced craving for alcohol in this model of alcoholization. The application of IHT at the beginning of alcohol intoxication as a protective agent delays the phase of formation of a pronounced craving for ethanol and reduces the amount of daily alcohol consumption. Conclusions. Thus, sessions of short-term hypoxic effects at the beginning of chronic ethanol consumption, provide an increase in the antioxidant defence of these animals relative to rats with alcohol intoxication.","PeriodicalId":52737,"journal":{"name":"Medichna nauka Ukrayini","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45710375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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