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Correlation of hemolysis by plasma hemoglobin with biochemical markers during storage of blood under standard conditions in the blood bank of a tertiary health-care center 一家三级医疗中心血库在标准条件下储存血液期间血浆血红蛋白溶血与生化指标的相关性
Pub Date : 2023-07-01 DOI: 10.4103/gjtm.gjtm_10_23
Parisha Gaur, Aparna Bhardwaj, Gaurav Raturi, Saqib Ahmed, Rana Usmani
Background and Objectives: Stored blood undergoes many metabolic, biochemical, and molecular changes known as storage lesions which are responsible for negative posttransfusion effects in recipients. This study aims to detect serial in vitro qualitative changes that occur during processing and blood storage including changes in plasma hemoglobin (Hb), plasma lactate dehydrogenase (LDH), and potassium levels and changes (fall) in pH of packed red blood cell at serial intervals under a standard condition of storage in the blood bank. This is a two year prospective study. Methods: Fifty donors were selected and blood units were collected and stored under blood bank conditions. Mean plasma Hb of stored blood was estimated by measuring the transmittance and absorbance of modified azide-methemoglobin and percentage hemolysis was calculated on days 0, 1, 7, 21, 28, 35, and 42 days. Similarly, plasma LDH and plasma potassium levels were also assessed during storage. Results: It was noted that free Hb level and percentage hemolysis progressively increased with storage along with the level of LDH and potassium. However, the extent of hemolysis did not exceed the permissible limit of up to 42 days of storage. Conclusion: It was concluded that quantitative estimation of Hb is superior to the visual method for the determination of hemolysis. Hemolysis can be further reduced by additive solution saline, adenine, glucose, and mannitol and using di-2-ethyl hexyl pHthalate as s plasticizer in blood bags for storage.
背景和目的:贮存的血液会发生许多代谢、生化和分子变化,这些变化被称为贮存病变,是受血者输血后出现不良反应的原因。本研究旨在检测血液处理和储存过程中发生的一系列体外质量变化,包括血库标准储存条件下血浆血红蛋白(Hb)、血浆乳酸脱氢酶(LDH)和钾水平的变化以及包装红细胞 pH 值的变化(下降)。这是一项为期两年的前瞻性研究。研究方法选取 50 名献血者,采集血液单位并在血库条件下储存。通过测量改良叠氮-血红蛋白的透射率和吸光度,估算储存血液的平均血浆血红蛋白,并在第 0、1、7、21、28、35 和 42 天计算溶血百分比。同样,还对储存期间的血浆 LDH 和血浆钾水平进行了评估。结果结果发现,游离血红蛋白水平和溶血百分比随 LDH 和钾水平的增加而逐渐增加。不过,溶血的程度并没有超过最多储存 42 天的允许限度。结论结论:在测定溶血时,Hb 的定量估计优于目测法。通过在血袋中添加生理盐水、腺嘌呤、葡萄糖和甘露醇溶液以及使用 pHthalate 二-2-乙基己酯作为增塑剂,可进一步减少溶血。
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引用次数: 0
Improving turnaround time for the issue of blood components in case of emergency 改善紧急情况下血液成分发放的周转时间
Pub Date : 2023-07-01 DOI: 10.4103/gjtm.gjtm_17_23
A. Basavarajegowda, J. Gnanaraj, Rajendra Gurunath Kulkarni, D. Sahoo
Background and Objectives: During a surgical or medical emergency, the timely issue of crossmatched blood is crucial for efficient and satisfactory patient revival. Turnaround time (TAT) is the duration taken from the time blood requests, and samples are received in the blood center until the blood is crossmatched and available for blood transfusion. The study aimed to audit the TAT in our center, analyze and study the factors delaying TAT, and formulate plans to improve it by implementing corrective measures. These actions would directly or indirectly impact the outcomes of the delivery of patient care in emergency services. Methods: A record-based prospective study was performed in a tertiary care teaching hospital with trauma and all emergency services in Southwestern India. TAT was calculated for all emergency blood requests received over 16 months in a systematic sampling method. Cases with prolonged TATs, where the product issue TAT exceeded the internal policy and quality indicator standards (15 min for emergency packed red blood cells issue), were further evaluated for causes of delay and analyzed at frequent intervals. Results: The mean TAT at the start of the study was 17.1 min (standard deviation [SD] 1.05 min), gradually reducing to 14.9 min (SD 0.62 min) by the end of the study, and it was statistically significant with P = 0.01. Conclusion: It is essential to deduce the causes for delayed TAT and work on those variables amenable to modifications based on priority to improve the TAT regarding pretransfusion testing and the issue of blood products, which is crucial in emergency services.
背景与目的:在外科或内科急诊中,及时发放交叉配血对于高效、满意地救治病人至关重要。周转时间(TAT)是指从血液中心收到血液申请和样本到血液交叉配血并可用于输血所需的时间。本研究旨在对本中心的周转时间进行审计,分析和研究延迟周转时间的因素,并制定计划,通过实施纠正措施来改善周转时间。这些措施将直接或间接地影响急诊服务中患者护理的结果。研究方法在印度西南部一家提供创伤和所有急诊服务的三级医疗教学医院开展了一项基于记录的前瞻性研究。采用系统抽样方法计算了 16 个月内收到的所有急诊用血申请的 TAT。对于产品发放 TAT 超过内部政策和质量指标标准(急诊包装红细胞发放时间为 15 分钟)的超长 TAT 病例,将进一步评估延误原因并定期进行分析。结果:研究开始时的平均 TAT 为 17.1 分钟(标准差 [SD] 1.05 分钟),研究结束时逐渐缩短至 14.9 分钟(标准差 0.62 分钟),P = 0.01,具有统计学意义。结论有必要推断出 TAT 延迟的原因,并根据优先顺序对那些可修改的变量进行修改,以改善输血前检测和血液制品问题方面的 TAT,这对急诊服务至关重要。
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引用次数: 0
Establishing a regional rare blood donor registry in Uttarakhand, India 在印度北阿坎德邦建立地区稀有血液捐献者登记处
Pub Date : 2023-07-01 DOI: 10.4103/gjtm.gjtm_28_23
M. Raturi, B. Adhikari, Y. Dhiman, D. Gaur, A. Kusum
Background and Objectives: Uttarakhand, a state in northern India, is home to various local communities, including the Garhwali, Kumaoni, and Jaunsari. In addition, individuals from neighboring countries such as Nepal and Tibet reside in Uttarakhand for many years, resulting in a diverse population. However, currently, there is no regional registry for rare blood donors in this state. The aim of this study was to develop a rare donor registry in Uttarakhand which could in future become a part of the national rare blood donor registry. Methods: During the routine donation process, samples were taken from randomly selected O-typed blood donors who are residents of Uttarakhand. Seventeen monoclonal antisera included Rh (anti-D, anti-C, anti-c, anti-E, and anti-e), Duffy (anti-Fya and anti-Fyb), Kell (anti-K and anti-k), Kidd (anti-Jka and anti-Jkb), Lewis (anti-Lea and anti-Leb), M, N, S, and s (Ortho Diagnostics Pvt Ltd, Mumbai, India). Furthermore, four polyclonal antisera from our serum repository, Diego (anti-Dia), Indian (anti-Ina), Rh (anti-Cw), and anti-Gp-Mur were used for phenotyping. The study was financially aided by the Uttarakhand State Council of Science and Technology (UCOST), Dehradun, Uttarakhand, Government of India. Results: Over a period of 16 months (November 2021–February 2023), out of a total of 17,004 blood donations, O blood type donors accounted for 29.9% (5085/17,004) of the donors screened. Among these, 7.3% (374/5085) of the donors were from the interior and plain areas of Uttarakhand. Our study revealed a high prevalence of k and e antigens, with 96.7% and 92.5%, respectively. The least prevalent antigen was Rh (Cw), observed in only 0.5% of cases. We also identified rare blood antigens such as Diego, Indian, and Gp-Mur and compared our findings with other studies. Furthermore, we identified a donor with an H-deficient phenotype, specifically the Bombay blood group (Oho). Conclusion: We have successfully established a regional registry for rare blood donors in Uttarakhand, which will be valuable for managing complex transfusion scenarios in the future. Further, given Uttarakhand's proximity to China and Nepal, the presence of individuals from these countries aptly connects the fields of anthropology and transfusion medicine.
背景与目标:北阿坎德邦是印度北部的一个邦,居住着各种当地社区,包括 Garhwali、Kumaoni 和 Jaunsari。此外,来自尼泊尔和西藏等邻国的人也常年居住在北阿坎德邦,从而形成了一个多样化的人口群体。然而,目前该邦还没有地区性的稀有献血者登记册。这项研究的目的是在北阿坎德邦建立一个稀有献血者登记处,将来成为全国稀有献血者登记处的一部分。方法:在常规献血过程中,从北阿坎德邦居民中随机抽取 O 型献血者样本。17 种单克隆抗血清包括 Rh(抗-D、抗-C、抗-E 和抗-e)、Duffy(抗-Fya 和抗-Fyb)、Kell(抗-K 和抗-k)、Kidd(抗-Jka 和抗-Jkb)、Lewis(抗-Lea 和抗-Leb)、M、N、S 和 s(Ortho Diagnostics Pvt Ltd,印度孟买)。此外,我们还使用了血清库中的四种多克隆抗血清:Diego(抗-Dia)、Indian(抗-Ina)、Rh(抗-Cw)和抗-Gp-Mur,用于表型分析。本研究得到了印度政府北阿坎德邦德拉敦的北阿坎德邦科技委员会(Uttarakhand State Council of Science and Technology, UCOST)的资助。研究结果在为期 16 个月(2021 年 11 月至 2023 年 2 月)的时间里,在总共 17004 名献血者中,O 型血献血者占筛选献血者的 29.9%(5085/17004)。其中,7.3%(374/5085)的献血者来自北阿坎德邦的内陆和平原地区。我们的研究显示,k 抗原和 e 抗原的流行率很高,分别为 96.7% 和 92.5%。发病率最低的抗原是 Rh 抗原(Cw),仅占 0.5%。我们还发现了迭戈、印度和 Gp-Mur 等罕见的血液抗原,并将我们的发现与其他研究进行了比较。此外,我们还发现了一名 H 抗原缺乏的捐献者,特别是孟买血型(Oho)。结论:我们成功地在北阿坎德邦建立了一个地区性稀有血型献血者登记处,这对今后管理复杂的输血情况非常有价值。此外,由于北阿坎德邦毗邻中国和尼泊尔,来自这些国家的献血者恰好将人类学和输血医学领域联系在一起。
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引用次数: 0
Zmiany demograficzne obserwowane w grupie polskich dawców zakwalifikowanych do oddania krwi i objętych badaniami przeglądowymi w kierunku czynników zakaźnych w latach 2005-2018 2005 年至 2018 年间,在一批有资格献血并接受传染病筛查的波兰献血者中观察到的人口统计学变化
Pub Date : 2023-06-30 DOI: 10.5603/jtm.95751
Dorota Kubicka-Russel, Aneta Kopacz, Ewa Sulkowska, Magdalena Łętowska, Piotr Grabarczyk
Wstęp: Dotychczas wykazano, że częstość zakażeń wśród dawców zakwalifikowanych do oddania krwi i objętych badaniami przeglądowymi, a w konsekwencji ryzyko powikłań potransfuzyjnych wiążą się z charakterystyką demograficzną dawców. Celem pracy było ustalenie zmian demograficznych wśród polskich dawców zakwalifikowanych w latach 2005–2018 do oddania krwi i objętych badaniami przeglądowymi w kierunku czynników zakaźnych. Uzyskane wyniki interpretowano w kontekście bezpieczeństwa transfuzji, a zwłaszcza ryzyka przenoszenia czynników zakaźnych przez krew. Materiał i metody: Analizowano dane gromadzone między innymi do oceny epidemiologii czynników zakaźnych przenoszonych przez krew: liczbę przebadanych dawców zakwalifikowanych do oddania krwi z podziałem na płeć, dawców pierwszorazowych i wielokrotnych oraz grupy wiekowe (≤ 20, 21–30, 31–40, 41–50, 51–60 i > 60 lat). Częstość (frakcję) wyrażano w procentach z 95-procentowym przedziałem ufności [95%CI], a różnice za pomocą punktu procentowego (p.p.). Istotność różnic (p < 0,05) weryfikowano za pomocą testu Chi-kwadrat, do oceny trendu stosowano współczynnik korelacji Spearmana (R). Wyniki: Większość dawców stanowili mężczyźni (średnio 74,07%), jednak w latach 2005–2012 udział kobiet wzrósł o 7 p.p. do 27,42% [27,30–27,53%] (p < 0,05); wśród dawców pierwszorazowych o 10,58 p.p., a wielokrotnych o 7,19 p.p. Największy udział kobiet obserwowano wśród najmłodszych dawców (36,02% [35,95–36,09%]), a najniższy wśród najstarszych dawców (14,14% [13,80–14,48%]) (różnica 21,88 p.p.; p < 0,05). Większość dawców krwi stanowili dawcy wielokrotni (średnio 66,78%). Udział dawców wielokrotnych wzrósł łącznie o 19,83 p.p. (p < 0,05): o 20,6 p.p. u mężczyzn i 21,15 p.p. u kobiet (dla obu grup p < 0,05). We wszystkich grupach wiekowych, z wyjątkiem dawców najmłodszych, większość (p < 0,05) stanowili dawcy wielokrotni. Udział dawców wielokrotnych rósł w kolejnych grupach wiekowych — od 36% u najmłodszych (≤ 20 lat) do 87% u dawców najstarszych (> 60 lat). Udział dawców w wieku > 40 lat zwiększył się o 11,58 p.p od 37,38% do 48,96%. Wnioski: W latach 2005–2018 obserwowano istotne zmiany demograficzne w grupie polskich dawców zakwalifikowanych do oddania krwi i objętych badaniami przeglądowymi, m.in. wzrósł udział kobiet oraz dawców wielokrotnych, co z punktu widzenia bezpieczeństwa transfuzji jest zjawiskiem korzystnym.
背景:迄今为止,已有研究表明,获得献血资格并被纳入复查的献血者中的感染率以及输血后并发症的风险与献血者的人口统计学特征有关。本研究的目的是确定 2005 年至 2018 年期间符合献血资格并被纳入感染病原体复查的波兰献血者的人口统计学变化。研究结果将在输血安全的背景下进行解释,尤其是血液传播传染性病原体的风险。材料和方法:分析了为评估血液传播传染病病原体的流行病学而收集的数据:按性别、首次献血者、重复献血者和年龄组(≤20、21-30、31-40、41-50、51-60 和 > 60 岁)分列的经筛查合格的献血者人数。频率(分数)以百分比和 95% 置信区间[95%CI]表示,差异以百分点(p.p.)表示。差异的显著性(p < 0.05)采用卡方检验(Chi-square test)进行验证,斯皮尔曼相关系数(R)用于评估趋势。结果大多数捐献者为男性(平均 74.07%),但在 2005 年至 2012 年期间,女性比例增加了 7 个百分点,达到 27.42% [27.30-27.53%] (p < 0.05);首次捐献者增加了 10.58 个百分点,多次捐献者增加了 7.19 个百分点。最年轻的献血者中女性比例最高(36.02% [35.95-36.09%]),最年长的献血者中女性比例最低(14.14% [13.80-14.48%])(两者相差 21.88 个百分点;p < 0.05)。大多数献血者是多次献血者(平均 66.78%)。重复献血者的比例增加了 19.83 个百分点(p < 0.05)。(p<0.05):男性增加了 20.6 个百分点,女性增加了 21.15 个百分点(两组均为 p<0.05)。在所有年龄组中,除最年轻的捐献者外,大多数人(p < 0.05)都是多次捐献者。在随后的年龄组中,多次捐献者的比例不断增加,从最年轻捐献者(20 岁以下)的 36% 增加到最年长捐献者(60 岁)的 87%。年龄在 40 岁以上的捐献者比例增加了 11.58 个百分点,从 37.38% 增加到 48.96%。结论:2005 年至 2018 年间,在波兰有资格献血并被纳入审查研究的献血者群体中观察到了显著的人口结构变化,包括女性和多次献血者比例的增加,从输血安全的角度来看,这是一个有利的现象。
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引用次数: 0
Impact of proliferative stress on both adaptive and innate immune response 增殖应激对适应性和先天免疫反应的影响
Pub Date : 2023-06-30 DOI: 10.5603/jtm.2023.0004
M. Czarnogórski, J. Witkowski, J. M. Zaucha
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引用次数: 0
Demographic changes in the Polish blood donors eligible for blood donation and screened for transfusion-transmitted infections (2005–2018) 符合献血条件和输血传播感染筛查的波兰献血者的人口变化(2005-2018年)
Pub Date : 2023-06-30 DOI: 10.5603/jtm.2023.0005
Dorota Kubicka‐Russel, Aneta Kopacz, E. Sulkowska, M. Łętowska, P. Grabarczyk
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引用次数: 0
Innovative applications of platelet derivatives in light of information presented during the 2022 virtual congress of the International Society of Blood Transfusion (ISBT); selected issues 根据2022年国际输血学会(ISBT)虚拟大会期间提供的信息,血小板衍生物的创新应用;选择的问题
Pub Date : 2023-03-31 DOI: 10.5603/jtm.2023.0003
Paulina Goczyńska, Joanna Lasocka, E. Lachert
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引用次数: 0
Hemostasis restoring in postpartum hemorrhage — algorithm 2023 产后出血的止血恢复-算法2023
Pub Date : 2023-03-31 DOI: 10.5603/jtm.2023.0001
E. Nowacka
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引用次数: 1
What’s new in the field of serum-based eye drops 以血清为基础的滴眼液领域有什么新进展
Pub Date : 2023-03-31 DOI: 10.5603/jtm.2023.0002
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引用次数: 1
Donor confidentiality and privacy: Need of the hour in this era of digitalization 捐助者的保密和隐私:数字化时代的时代需要
Pub Date : 2023-01-01 DOI: 10.4103/2468-8398.376689
Shivaram Chandrashekar
{"title":"Donor confidentiality and privacy: Need of the hour in this era of digitalization","authors":"Shivaram Chandrashekar","doi":"10.4103/2468-8398.376689","DOIUrl":"https://doi.org/10.4103/2468-8398.376689","url":null,"abstract":"","PeriodicalId":52961,"journal":{"name":"Global Journal of Transfusion Medicine","volume":"25 1","pages":"1 - 3"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72767055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
Global Journal of Transfusion Medicine
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