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Pengukuran Stok Karbon Ekosistem Lamun Di Kawasan Taman Nasional Baluran, Jawa Timur
Pub Date : 2022-09-02 DOI: 10.14710/jkt.v25i3.13520
Dadan Zulkifli, Heri Triyono, R. Suharti, M. A. Jabbar, Aditya Bramana, Siti Mira Rahayu, I. N. Sudiarsa, Lilyani Gita Herawati
 Climate change caused by various activities of human activity has produced carbon dioxide gas affecting global warming. Seagrass ecosystem has the ability to absorb and store large quantities of carbon that can reduce carbon emissions. Objectives of this study is to assess seagrass population structure, assess carbon deposits estimation and water quality parameters. The method used in this research is purposive sampling method and location determination used line Transect quadrant method which refers to LIPI method. Carbon measurements in seagrass samples used LOI method. Seagrass species found in the waters of Baluran National Park are Enhalus acoroides, Thalassia hemprichii, Cymodocea rotundata, Cymodocea serrulata, Syringodium isoetifolium, Halophila minor, Halophila ovalis dan Halodule uninervis. The seagrass Enhalus acoroides has a highest biomass value and an estimate of carbon deposits 37.817 gC/m2. The total estimate of the highest carbon stock is obtained at the bottom of the substrate 40.063 ton.  Perubahan iklim yang disebabkan oleh berbagai aktifitas kegiatan manusia menghasilkan gas karbon diokasida yang berdampak pada pemanasan global. Ekosistem padang lamun memiliki kemampuan untuk menyerap dan menyimpan karbon dalam jumlah besar yang dapat mengurangi emisi karbon. Tujuan penelitian ini adalah: 1) mengkaji struktur populasi lamun, 2) mengkaji estimasi simpanan karbon, 3) mengkaji parameter kualitas air di Taman Nasional Baluran. Metode yang digunakan yaitu purposive sampling method dan penentuan lokasi menggunakan metode line transect quadrant yang mengacu pada metode LIPI. Pengukuran kualitas air dilakukan secara langsung di lapangan sesuai Standar Nasional Indonesia (SNI) dan pengukuran karbon pada sampel lamun menggunakan metode LOI. Berdasarkan hasil penelitian, diketahui bahwa Spesies lamun yang ditemukan di perairain Taman Nasional Baluran adalah Enhalus acoroides, Thalassia hemprichii, Cymodocea rotundata, Cymodocea serrulata, Syringodium isoetifolium, Halophila minor, Halophila ovalis dan Halodule uninervis. Jenis lamun Enhalus acoroides mempunyai nilai biomassa dan estimasi simpanan karbon paling tinggi yaitu 37,817 gC/m2. Estimasi total stok karbon tertinggi didapatkan pada bagian bawah substrat (rhizoma dan akar) sebesar 40,063 ton.
气候变化引起的各种人类活动产生了影响全球变暖的二氧化碳气体。海草生态系统具有吸收和储存大量碳的能力,可以减少碳排放。本研究的目的是评估海草种群结构,评估碳储量估计和水质参数。本研究所使用的方法是有目的的取样方法和使用线-断面象限法的位置确定方法,即LIPI方法。海草样品中的碳测量采用LOI法。在巴鲁兰国家公园水域发现的海草种类有:橡果海鞘(Enhalus acoroides)、海鞘海鞘(Thalassia hemprichii。Acroides海草的生物量最高,估计碳储量为37817克/平方米。最高碳储量的总估算值是在基质底部40.063吨处获得的。由各种人类活动引起的气候变化会产生影响全球变暖的二氧化碳气体。海洋生态系统具有大量吸收和储存碳的能力,可以减少碳排放。本研究的目的是:1)研究贫民窟人口的结构,2)研究碳储量的估计,3)研究国家废物公园的水质参数。所使用的方法是有目的的采样方法和使用横断面象限法的位置确定,该方法指的是LIPI方法。根据印尼国家标准(SNI)直接在现场进行水质测量,并使用LOI方法对泥浆样品进行碳测量。根据研究结果,已知在巴鲁兰国家公园水域发现的古代物种有:橡果Enhalus acoroides、铁杉Thalassia hemprichii、圆腹环孢Cymodocea rotundata、细腹环孢Cymodocea serrulata、等翅Syringodium isotifolium、小嗜盐藻Halophila minor、卵形嗜盐藻Halophila ovalis和uninervis Halodule。Enhalus acoroides羔羊的生物量值和碳储量估计值最高,为37817克/平方米。最高碳储量的总估算值是在40063吨的基质(根茎和根)底部获得的。
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引用次数: 0
Pengaruh Tingkat Kerapatan dan Kedewasaan Hutan Mangrove dalam Memerangkap Sedimen di Muara Sungai Langsa Kota Langsa 红树林铁路与成长铁路铁路铁路铁路
Pub Date : 2022-09-02 DOI: 10.14710/jkt.v25i3.14009
Erlangga Erlangga, Helmi Gusnita, S. Syahrial, C. M. 'Akla, Imamshadiqin Imamshadiqin, Riri Ezraneti, Rian Firdaus
In November 2021, a study on the effect of mangrove density and maturity level on sediment trapping in the Langsa River estuary, Langsa City, was conducted with the objective of determining the density, maturity, and rate of sediment deposition around the mangrove forest, as well as the relationship between mangrove density and maturity level and sediment deposition rate. Mangrove vegetation was collected using a 40 m line transect parallel to the river flow and a 10 x 10 m plot, while sediment samples were collected using a sediment trap. The study's findings indicated that mangrove density varied between 675.00 and 1541.67 ind/ha, that mangrove maturity was uneven, with trunk diameters ranging between 4.14 and 9.83 cm and basal areas ranging between 30.45 and 72.93 m2/ha. Meanwhile, sediment deposition rates ranged between 26.94 and 277.73 mg/cm2/day, with a strong correlation between density and maturity of mangrove vegetation (98% and 99%, respectively).   Penelitian terhadap pengaruh tingkat kerapatan dan kedewasaan hutan mangrove dalam memerangkap sedimen di muara Sungai Langsa Kota Langsa dilakukan pada bulan November 2021 dengan tujuan untuk mengetahui tingkat kerapatan, kedewasaan dan laju pengendapan sedimen di sekitar hutan mangrovenya serta untuk mengetahui keterkaitan antara tingkat kerapatan dan kedewasaan hutan mangrove terhadap laju pengendapan sedimennya. Vegetasi mangrove dikumpulkan dengan menarik transek garis sepanjang 40 m yang sejajar dengan aliran sungai dan dibuat plot berukuran 10 x 10 m, sedangkan sampel sedimennya diambil menggunakan sediment trap. Hasil penelitian memperlihatkan bahwa kerapatan mangrovenya berkisar antara 675.00 – 1541.67 ind/ha, kedewasaan mangrovenya tidak merata dengan diameter batangnya berkisar antara 4.14 – 9.83 cm dan basal areanya berkisar antara 30.45 – 72.93 m2/ha. Sementara untuk laju pengendapan sedimennya berkisar antara 26.94 – 277.73 mg/cm2/hari, dimana keterkaitan antara kerapatan dan kedewasaan vegetasi mangrovenya memiliki hubungan yang kuat (masing-masing 98% dan 99%).
2021年11月,在Langsa市Langsa河口进行了一项关于红树林密度和成熟度水平对沉积物捕获的影响的研究,目的是确定红树林周围的密度、成熟度和沉积物沉积速率,以及红树林密度与成熟度水平和沉积物沉积率之间的关系。红树林植被采用平行于河流流量的40 m样带和10 x 10 m地块进行采集,沉积物样品采用沉积物捕集器进行采集。研究结果表明,红树林密度在675.00至1541.67 ind/ha之间,红树林成熟度不均衡,树干直径在4.14至9.83厘米之间,基底面积在30.45至72.93平方米/ha之间。同时,沉积物沉积率在26.94至277.73 mg/cm2/天之间,红树林植被的密度和成熟度之间有很强的相关性(分别为98%和99%)。2021年11月,为了确定红树林肥力水平,对郎萨河流域红树林肥力和沉积物截留肥力的影响进行了研究,红树林周围沉积物流动的丰富程度和速度,并了解红树林的密度和丰富程度与沉积物流动速度之间的相关性。红树林植被的采集方法是沿着河流拉一条40米长的样带,绘制10 x 10米的图,而沉积物样本则是使用沉积物捕集器采集的。研究表明,红树林密度在675.00至1541.67 ind/ha之间,红树林密度不受棒直径4.14至9.83cm的限制,其基底面积在30.45至72.93 m2/ha之间。而对于代谢率,最小范围为26.94至277.73 mg/cm2/天,其中红树林植被的密度与疲劳度之间的相关性很强(分别为98%和99%)。
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引用次数: 2
Komunitas Spons (Porifera) pada Ekosistem Terumbu Karang di Manokwari, Indonesia 印度尼西亚Manokwari最伟大的Karang生态系统上的海绵群落(Porifera)
Pub Date : 2022-09-02 DOI: 10.14710/jkt.v25i3.14098
Nelly Sayori, T. Tururaja, Duaitd Kolibongso
Sponges are one of the most influential benthic organisms in coral reef ecosystems. Many studies on sponge communities have been carried out globally, from the tropics to the sub-tropics. However, in Indonesia, the sponge community has not been sufficiently observed, especially its diversity and interactions with habitats. Manokwari, a developing city north of the Bird's Head Seascape region, Papua has a lack of information on benthic communities and no reports of sponges. This study is to examine the sponge community (diversity and distribution) in coral reef ecosystem. This study found that sponge richness (species and morphology) was categorized as “low”, with only 11 species with 8 morphological forms. The most common species included Niphates erecta, Stylissa carteri, and Pseudoceratina purpurea, while the most common growth forms were massive and encrusting, accounting respectively for 27.3% and 18.2% of the total number of species. The highest diversity was found on the island of Kaki (5 species) with the island of Nusmapi having an uneven distribution of sponges. The results of our study found that there was no relationship between sponge diversity and morphology. This baseline information is essential for management of marine biodiversity hotspots in taking decisions for marine life conservation.  Spons merupakan salah satu organisme bentik yang paling berpengaruh dalam ekosistem terumbu karang. Banyak penelitian tentang komunitas spons telah dilakukan secara global, dari daerah tropis hingga sub tropis. Namun di Indonesia, komunitas spons belum cukup diamati, terutama keanekaragaman dan interaksinya dengan habitat. Manokwari, kota berkembang di utara dari wilayah Bentang Laut Kepala Burung (BLKB), Papua memiliki kekurangan informasi tentang komunitas bentik dan tidak ada laporan tentang spons. Studi ini untuk mengkaji komunitas spons (keanekaragaman dan distribusi) pada ekosistem terumbu karang. Penelitian ini menemukan kekayaan spons (spesies dan morfologi) yang dikategorikan “rendah”, dengan hanya 11 spesies dengan 8 bentuk morfologi. Spesies yang paling umum termasuk Niphates erecta, Stylissa carteri, dan Pseudoceratina purpurea, sedangkan bentuk pertumbuhan yang paling umum adalah massif dan encrusting dengan menyumbang masing-masing 27,3% dan18,2% dari total jumlah spesies. Keanekaragaman tertinggi ditemukan di pulau Kaki (5 spesies) dengan pulau Nusmapi memiliki sebaran spons tidak merata. Hasil penelitian kami menemukan tidak ada hubungan antara keanekaragaman spons dengan bentuk morfologi. Informasi dasar ini sangat penting untuk pengelolaan hotspot keanekaragaman hayati dalam perumusan keputusan untuk konservasi biota laut.
海绵是珊瑚礁生态系统中最具影响力的底栖生物之一。从热带到亚热带,全球范围内都开展了许多关于海绵群落的研究。然而,在印度尼西亚,海绵群落尚未得到充分观察,尤其是其多样性以及与栖息地的相互作用。巴布亚鸟头海景区北部的发展中城市马诺夸里缺乏关于底栖生物群落的信息,也没有海绵的报告。本研究旨在考察珊瑚礁生态系统中的海绵群落(多样性和分布)。这项研究发现,海绵的丰富度(物种和形态)被归类为“低”,只有11种,有8种形态。最常见的物种包括直立Niphates erecta、Stylisa carteri和Pseudoceratina purpurea,而最常见的生长形式为块状和包壳,分别占总物种数的27.3%和18.2%。卡基岛(5种)的海绵多样性最高,努斯马皮岛的海绵分布不均匀。我们的研究结果发现,海绵多样性与形态之间没有关系。这一基线信息对于海洋生物多样性热点的管理和海洋生物保护决策至关重要。UNK][UNK]海绵是珊瑚礁生态系统中最具影响力的底栖生物之一。从热带到亚热带,全球范围内都对赞助商社区进行了大量研究。但在印度尼西亚,赞助者群体没有得到充分的观察,尤其是多样性及其与栖息地的互动。Manokwari,该市位于巴布亚鸟头海底(BLKB)地区的北部,缺乏有关底栖生物群落的信息,也没有赞助报告。本研究旨在研究珊瑚礁生态系统的赞助者群落(多样性和分布)。这项研究发现,海绵的丰富性(物种和形态)被归类为“低”,只有11种,具有8种形态。最常见的物种包括直立Niphates erecta、carteri Stylisa和purpurea Pseudoceratina,而最常见的生长形式是巨大和结壳,分别占物种总数的27.3%和18.2%。在富特岛(5种)和努斯马皮岛上发现的多样性最高的海绵有着无限的分布。我们的研究发现,海绵多样性与形态之间没有相关性。这些基本信息对于海洋生物群保护决策中生物多样性热点的管理非常重要。
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引用次数: 0
Biomassa dan Stok Karbon pada Ekosistem Padang Lamun di Pulau Pamegaran, Taman Nasional Kepulauan Seribu 1000群岛国家公园太平洋银河系生态系统的生物量和碳储量
Pub Date : 2022-09-02 DOI: 10.14710/jkt.v25i3.14030
S. Santoso, Ratih Ida Adharini
Seagrass is one of the important ecosystems in coastal areas. This study aims to determine the biomass and carbon stock in the seagrass ecosystems of the Pamegaran Island, Seribu Islands National Park, Jakarta. Data collection was conducted on December 2021 to January 2022 is done by using SeagrassWatch method and carbon data sampling results were analyzed by Kurmies method / SNI 13-4720-1998 method. Cymodocea rotundata has the highest biomass value, while Halodule uninervis has the lowest biomass value. The seagrass biomass value at the bottom of the substrate was higher than the top of the substrate in all the species found was because the rhizome contained carbohydrates and nutrients produced in the photosynthesis process and stored at the bottom of the substrate. The total value of seagrass carbon stock in the entire area is 1,932,151.36 ± 265,280.90 g C or 1.932 ± 2.652 t C with an area of 3.63 ha of seagrass beds. Thalassia hemprichii has the highest carbon stock value, while Halodule uninervis species has the lowest biomass value. The carbon stock value of the bottom of the substrate was higher than that of the top of the substrate in all species found.   Lamun adalah salah satu ekosistem penting yang ada di daerah pesisir. Penelitian ini bertujuan untuk mengetahui biomassa dan stok karbon pada ekosistem padang lamun di Pulau Pamegaran, Taman Nasional Kepulauan Seribu, Jakarta. Pengambilan data dilakukan pada bulan Desember 2021 hingga Januari 2022 menggunakan metode SeagrassWatch dan analisis data karbon menggunakan metode Kurmies / metode SNI 13-4720-1998. Cymodocea rotundata memiliki nilai biomassa yang tertinggi, sedangkan Halodule uninervis memiliki nilai biomassa terendah. Nilai biomassa lamun bagian bawah substrat lebih tinggi dari bagian atas substrat pada semua spesies yang ditemukan hal ini karena rhizome mengandung karbohidrat serta zat hara yang dihasilkan pada proses fotosintesis dan tersimpan pada bagian bawah substrat. Nilai total stok karbon lamun pada keseluruhan luas wilayah sebesar 1.932.151,36 ± 265.280,90 g C atau 1,932 ± 2,652 t C dengan luas wilayah padang lamun sebesar 3,63 Ha. Thalassia hemprichii memiliki nilai stok karbon tertinggi, sedangkan Halodule uninervis memiliki nilai biomassa terendah. Nilai stok karbon bagian bawah substrat lebih tinggi dari bagian atas substrat pada semua spesies yang ditemukan.
海草是沿海地区重要的生态系统之一。本研究旨在确定雅加达塞里布群岛国家公园帕梅加兰岛海草生态系统中的生物量和碳储量。数据收集于2021年12月至2022年1月,采用SeagrasWatch方法进行,碳数据采样结果采用Kurmies方法/SNI 13-4720-1998方法进行分析。轮藻的生物量值最高,而单茎卤虫的生物量价值最低。在所有发现的物种中,基质底部的海草生物量值都高于基质顶部,这是因为根茎含有光合作用过程中产生并储存在基质底部的碳水化合物和营养物质。整个地区的海草碳储量总值为1932151.36±265280.90 g C或1932±2652 t C,海草床面积为3.63公顷。铁藻具有最高的碳储量值,而单生卤虫物种具有最低的生物量值。在所有发现的物种中,基质底部的碳储量值都高于基质顶部的碳储量。拉蒙是沿海地区最重要的生态系统之一。这项研究旨在寻找雅加达千岛国家公园大陆海洋生态系统中的生物量和碳储量。2021年12月至2022年1月期间使用SeagrasWatch方法进行的数据收集,以及使用Kurmies/SNI方法13-4720-1998进行的碳数据分析。圆形环斑藻的生物量值最高,而单茎环斑藻生物量值最低。在所有被发现的物种中,基质底部的羔羊生物量值都高于基质顶部,因为根茎含有碳水化合物和光合作用过程中产生并储存在基质底部的原料。1932151.36±265280,90 g C或1932±2652 t C的整个宽平原地区的煤炭储量总值为3.63 ha。铁藻的碳储量价值最高,而紫苏的生物量价值最低。在所有发现的物种中,基底底部的碳存量值都高于基底顶部的碳存量。
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引用次数: 0
Peranan Taurin pada Pertumbuhan dan Kelangsungan Hidup Larva Ikan Kerapu Bebek Cromileptes altivelis (Valenciennes, 1828) 牛磺酸在Larva Cromileptes altivelis生长和存活中的作用(Valenciennes,1828)
Pub Date : 2022-09-02 DOI: 10.14710/jkt.v26i1.13490
Regina Melianawati, Ni Wayan Widya Astuti, Tridjoko Tridjoko
Taurine is an important amino acid for the growth of marine fish larvae The humpback grouper (Cromileptes altivelis) is a highly valuable marine fish that has been farmed in hactheries. The increasing of larval growth and survival rate of humpback grouper was carried out by taurine addition as enrichment ingredient to zooplankton as live feed for larvae. The purpose of this study was to find out the role of taurine on larval growth and survival rate. Two treatments tested were taurine addition by bioencapsulated through zooplankton rotifers Brachionus rotundiformis (A) and without taurine addition in rotifers (B). The observed parameters were larval growth and survival rate. Larval samples were taken on day 1 and 5, then continued every 5 days until the end of the study, by using10 larval samples at each time. The results showed that mostly all  larvae have already metamorphosed and become juveniles at 35 days old. At that time, larvae with taurine addition (A) have bigger in size, faster growth and also higher survival rate compared than larvae without taurine addition(B). Therefore, the result of this study indicated that the addition of taurine could improve larval growth and the survival rate of humpback grouper.   Taurin merupakan asam amino penting untuk pertumbuhan larva ikan laut. Ikan kerapu bebek (Cromileptes altivelis) adalah salah satu jenis ikan laut bernilai ekonomis tinggi yang sudah dapat dibudidayakan. Peningkatan pertumbuhan dan kelangsungan hidup larva ikan kerapu bebek dilakukan dengan penambahan taurin sebagai bahan pengkaya ke dalam zooplankton sebagai pakan alami larva. Tujuan penelitian ini adalah mengetahui peranan taurin pada pertumbuhan dan kelangsungan hidup larva. Dua perlakuan yang diujikan adalah penambahan taurin melalui bioenkapsulasi pada zooplankton rotifer Brachionus rotundiformis (A) dan tanpa penambahan taurin pada rotifer (B). Parameter yang diamati adalah pertumbuhan dan kelangsungan hidup larva. Sampel larva diambil pada umur 1 dan 5 hari,  selanjutnya setiap 5 hari sekali hingga akhir penelitian, dengan menggunakan 10 ekor larva setiap waktu pengambilan sampel.  Hasil penelitian menunjukkan bahwa sebagian besar populasi larva telah bermetamorfosis menjadi juvenil pada umur 35 hari. Pada umur tersebut, larva yang diberi penambahan taurin (A) memiliki ukuran yang lebih besar, pertumbuhan yang lebih cepat dan tingkat kelangsungan hidup yang lebih tinggi dibandingkan dengan larva yang tidak diberi penambahan taurin (B). Oleh karenanya, hasil penelitian ini menunjukkan bahwa penambahan taurin pada rotifer dapat meningkatkan pertumbuhan dan kelangsungan hidup larva ikan kerapu bebek.  
牛磺酸是海洋鱼类幼体生长的重要氨基酸座头石斑鱼(Cromileptes altivelis)是一种非常有价值的海洋鱼类,已在哈彻里养殖。在浮游动物中添加牛磺酸作为富集成分,作为座头石斑鱼幼体的活饲料,可以提高其幼体的生长和存活率。本研究旨在了解牛磺酸对幼虫生长和存活率的影响。试验的两种处理是通过浮游动物轮虫生物胶囊添加牛磺酸(A)和在轮虫中不添加牛磺酸(B)。观察到的参数是幼虫的生长和存活率。在第1天和第5天采集幼虫样本,然后每5天持续一次,每次使用10个幼虫样本,直到研究结束。结果表明,大多数UNK幼虫在35天大时就已经变质并成为幼体。此时,添加牛磺酸(A)的幼虫与未添加牛磺酸(B)的幼虫相比,体积更大,生长更快,存活率也更高。因此,本研究结果表明,添加牛磺酸可以提高座头石斑鱼的幼体生长和存活率。牛磺酸是海鱼幼体生长的重要氨基酸。高鳍海鱼是一种经济上非常可行的海鱼。通过将牛磺酸作为一种丰富的成分作为天然幼虫添加到浮游动物中,可以提高鸭子常见鱼类幼虫的生长和存活率。本研究的目的是了解牛磺酸在幼虫生长和存活中的作用。试验的两种处理是通过生物包膜向浮游动物圆尾轮虫中添加牛磺酸(A)和不向轮虫中加入牛磺酸(B)。观察到的参数是幼虫的生长和存活。在1天和5天大时采集幼虫样本,然后每5天采集一次,直到研究结束,每次采样使用10条幼虫尾巴。结果表明,大多数幼虫种群在35日龄时已经代谢为幼体。在该年龄,添加到幼虫(A)中的幼虫比未添加到幼虫中的幼虫(B)体积更大,生长更快,存活率更高。因此,本研究的结果表明,在轮虫中添加牛磺酸可以提高鸭常见鱼类幼虫的生长和存活率。
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引用次数: 0
Fraksinasi Flavonoid Spirulina platensis dengan Metode Kromatografi Lapis Tipis dan Aktivitas Inhibisi Enzim α-Glukosidase Fraksinasi类黄酮Spirulina platensis薄层色谱方法和活动的抑制酶α-Glukosidase
Pub Date : 2022-09-02 DOI: 10.14710/jkt.v25i3.13905
Hartoyo Notonegoro, H. Djamaludin, I. Setyaningsih, Kustiariyah Tarman
Spirulina platensis is a type of Cyanobacterium microalgae that forms multicellular helicoidal filaments. Spirulina platensis contains primary and secondary metabolites. The type and amount of the active compound Spirulina platensis depends on the method of extraction, fractionation, and isolation. So far, there are not much research data related to the active compound of Spirulina platensis extract cultured on Walne. The purpose of this study was to use of thin layer chromatography (TLC) method to separate flavonoids from Spirulina platensis biomass extract cultured on Walne media and the activity of a-glucosidase enzyme inhibition using biomass, crude extract, active fraction of flavonoids and phycocyanins from Spirulina platensis. This research method is descriptive experimental, which the Spirulina platensis is cultured on 80 g/L NaNO3 modified Walne media, extracted by maceration, fractionated by TLC and isolated the active compound by Preparative TLC (PTLC). The analysis included fraction and isolation of flavonoids from Spirulina platensis. The results showed that the TLC could be used to identify the active compound of Spirulina platensis extract cultured on Walne. Fractionation of Spirulina platensis extract using stationary phase silica gel Si 60 GF254 and the best mobile phase with a combination of chloroform:ethyl acetate (6:4) and an Rf1 value of 0.58; Rf2 0.71; and Rf3 0.83, as well as yellow-orange spots. Isolation of the active compound of Spirulina platensis extract using PLTC stationary phase silica gel Si 60 PF254 and the best mobile phase combination eluent chloroform:ethyl acetate (9:1). RF value of Rf2 0.57; Rf3 0.86; and Rf4 0.93 with dark yellow-brown spots. The color of the spots from the PLTC results shows that the active compounds of Spirulina platensis extract are flavonoid compounds. Biomass, crude extract, phycocyanine extract and flavonoids from Spirulina do not have inhibitory activity against α-glucosidase enzyme.  Spirulina platensis merupakan jenis mikroalga Cyanobacterium yang membentuk filamen helicoidal multiseluler. Spirulina platensis mengandung senyawa metabolit primer dan sekunder. Jenis dan jumlah senyawa aktif Spirulina platensis tergantung pada metode ekstraksi, fraksinasi dan isolasi. Sejauh ini belum banyak data hasil penelitian terkait senyawa aktif ekstrak biomassa Spirulina platensis yang dikultur pada media Walne. Tujuan penelitian ini yaitu penggunaan metode kromatografi lapis tipis (KLT) untuk memisahkan flavonoid ekstrak biomassa Spirulina platensis yang dikultur pada media Walne serta aktivitas inhibisi enzim α-glukosidase menggunakan biomassa, ekstrak kasar, fraksi aktif flavonoid dan fikosianin dari Spirulina platensis. Metode penelitian ini adalah eksprimental deksriptif dimana mikroalga Spirulina platensis dikultur pada media Walne modifikasi 80 g/L NaNO3, diekstraksi dengan maserasi, difraksinasi dengan KLT dan diisolasi senyawa aktif dengan KLT Preparatif (KLTP). Analisis yang dilakukan
钝顶螺旋藻是一种形成多细胞螺旋状细丝的蓝藻微藻。螺旋藻含有初级和次级代谢产物。活性化合物螺旋藻的类型和含量取决于提取、分级和分离的方法。到目前为止,还没有太多关于在沃尔恩培养的钝顶螺旋藻提取物活性化合物的研究数据。本研究采用薄层色谱法从Walne培养基上培养的钝顶螺旋藻生物质提取物中分离黄酮类化合物,并利用钝顶螺旋藻的生物质、粗提物、黄酮类化合物和藻蓝蛋白的活性组分对a-葡萄糖苷酶的抑制活性进行了研究。本研究采用描述性实验方法,将螺旋藻在80g/L NaNO3修饰的Walne培养基上培养,浸渍提取,TLC分级,制备TLC(PTLC)分离活性成分。分析了钝顶螺旋藻中黄酮类化合物的组分和分离。结果表明,用薄层色谱法可对培养基螺旋藻提取物中的活性成分进行鉴别。使用固定相硅胶Si60GF254和最佳流动相与氯仿:乙酸乙酯(6:4)和Rf1值为0.58的组合对钝顶螺旋藻提取物进行分级;Rf2 0.71;Rf3为0.83以及黄橙色斑点。用PLTC固定相硅胶Si60PF254和最佳流动相组合洗脱剂氯仿:乙酸乙酯(9:1)分离螺旋藻提取物的活性化合物。射频值Rf2 0.57;Rf3 0.86;Rf4 0.93,具有暗黄棕色斑点。PLTC结果表明,螺旋藻提取物的活性成分为黄酮类化合物。螺旋藻的生物量、粗提物、藻蓝蛋白提取物和黄酮类化合物对α-葡萄糖苷酶无抑制活性。钝顶螺旋藻是一种具有螺旋状多细胞丝的蓝藻。钝顶螺旋藻是一种代谢型引物。钝顶螺旋藻的数量和数量是在低温、高温和低温条件下产生的。目前有大量数据表明,媒体对钝顶螺旋藻的生物特性进行了研究。这项研究的目的是利用Klomatografi lapis tipis(KLT)方法来制备在培养基上培养的螺旋藻生物提取物类黄酮,并通过利用螺旋藻的生物提取物、提取物、黄酮类化合物和黄酮类化合物抑制α-葡萄糖苷酶活性。本研究的主要目的是将螺旋藻培养基改性为80 g/L NaNO3,用微波、KLT和KLT制剂(KLTP)进行二异处理。所进行的分析包括对螺旋藻中黄酮类化合物的分析和分离。这项研究表明,KLT可以用来识别媒体报道的钝顶螺旋藻的危害。钝顶螺旋藻弱毒株使用直径为60 GF254的硅胶,其组合形式为:初始值(6:4)和初始值Rf1 0,58;Rf2 0,71;Rf3 0.83,以及一个很小的洞。螺旋藻分离株使用直径为Si60 PF254的KLTP凝胶,并在低温条件下(9:1)混合培养。Nilai Rf2 0,57;Rf3 0.86;Rf4 0.93与焦炭凝胶的水溶性有关。警告说,KLTP使螺旋藻中的人参成分含有黄酮类化合物。螺旋藻的生物测定法、生物活性、生物活性和类黄酮不具有抑制α-葡聚糖酶活性。
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引用次数: 0
Molecular Identification of Brown Algae Sargassum sp. from the Lombok Coastal Waters 龙目岛近海褐藻马尾藻的分子鉴定
Pub Date : 2022-06-21 DOI: 10.14710/jkt.v25i3.14760
Yeni Sulistiyani, N. Afiati, H. Haeruddin, A. Sabdono
Sargassum is a well-known genus of brown algae in Indonesia that has long been investigated due to its economic importance. To support its biological research, it is important to identify and classify the species studied. Morphological identification had greatly contributed to taxonomy, however, it cannot distinguish Sargassum species accurately due to its plasticity. The current study aims to identify Lombok Sargassum molecularly using an ITS2 DNA barcode. Fresh algae were collected from Ekas Bay (EB) and Aan Cape (AC) in Lombok. DNA was first purified, and then its gene product was amplified using ITS2 primers. DNA sequences were examined and traced using the Basic Local Alignment Search Tool (BLAST). DNA sequences were processed in MEGA-X to reconstruct the phylogenetic tree and estimate the genetic distance. Three species were identified based on the BLAST results: Sargassum cf granuliferum, Sargassum polycystum, and Sargassum oligocystum. The base length obtained ranged from 521 to 637 bp, with a similarity percentage of 99.25 to 100%. The phylogenetic tree exhibited each recognized Sargassum species was clustered with the same species from the gene bank. Interspecies genetic distance was 0,000-0,0039, while amongst Sargassum species it's 0,0136-0,2395. The genetic distance between Sargassum and Ulva adherens (outgroup) was >1. Sargassum species found in Lombok were closely related to other similar species in the GenBank.
马尾藻是印度尼西亚一种众所周知的褐藻属,由于其经济重要性,人们对其进行了长期的研究。为了支持其生物学研究,对所研究的物种进行识别和分类是很重要的。形态鉴定为马尾藻的分类做出了重要贡献,但由于马尾藻的可塑性,形态鉴定不能准确区分马尾藻的种类。目前的研究旨在利用ITS2 DNA条形码从分子上识别龙目岛马尾藻。在龙目岛的Ekas湾(EB)和Aan角(AC)采集了新鲜藻类。首先纯化DNA,然后用ITS2引物扩增其基因产物。使用基本局部比对搜索工具(BLAST)检查和追踪DNA序列。在MEGA-X中对DNA序列进行处理,重建系统发育树并估计遗传距离。根据BLAST结果鉴定出3种:马尾藻(Sargassum cf granulliferum)、马尾藻(Sargassum polycystum)和马尾藻(Sargassum oligocystum)。得到的碱基长度为521 ~ 637 bp,相似度为99.25 ~ 100%。系统发育树显示,每个识别的马尾藻物种都与基因库中的相同物种聚集在一起。种间遗传距离为0 ~ 0 0039,马尾藻种间遗传距离为0 0136 ~ 0 2395。马尾藻与尾藻(外群)的遗传距离为1.91。龙目岛发现的马尾藻种类与GenBank中其他类似物种有密切的亲缘关系。
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引用次数: 0
Karakteristik Arus Laut Permukaan dari High Frequency Radar pada Musim Timur di Selat Bali 巴厘岛南部东部高频雷达的地面流特征
Pub Date : 2022-06-20 DOI: 10.14710/jkt.v25i3.13978
Shafira Tsanyfadhila, Aris Ismanto, M. Helmi
Bali Strait has many activities in port and water, such as passenger and freight transportation, tourism, and fisheries. Oceanographic conditions, especially sea surface current (SSC), affect the smoothness of ports and shipping activities in the Bali Strait. High-Frequency Radar (HF Radar) has the advantage of monitoring and mapping surface currents and ocean waves with high resolution. Because of the narrow strait area, HF Radar is very beneficial in describing the characteristics of SSC in the Bali Strait. Therefore, HF Radar data is used to describe the dynamics of SSC in the Bali strait, especially in ship crossing lane, with a temporal approach during Southeast Monsoon, June to August 2020. This study was conducted for 15 days each month which included neap tide and spring tide. Surface current charts are created to analyze the pattern of temporal SSC against the wind, tides, and their components. The results showed In the southeast monsoon, the average current speed at the research site ranged from 0.1 – 1.08 m/s. The direction of the surface current is predominantly towards the south. The characteristics of surface currents in the Bali Strait are influenced by tides, sea level anomalies, and coastal morphology. Temporally, the speed of the current is greater during the ebb phase and when the spring tide. Spatially, the central region of the Bali Strait waters has a greater current speed. Knowledge of areas and times with higher current speeds can be the basis for determining the shipping lane between ports in the Bali Strait.  Selat Bali memiliki banyak kegiatan di pelabuhan dan perairan, seperti transportasi penumpang dan barang, pariwisata, serta perikanan. Kondisi oseanografi, khususnya arus permukaan laut, mempengaruhi kelancaran pelabuhan dan aktivitas pelayaran di Selat Bali. High-Frequency Radar (HF Radar) memiliki keunggulan pemantauan dan pemetaan arus permukaan dan gelombang laut dengan resolusi tinggi. Karena wilayah selat yang sempit, HF Radar sangat bermanfaat dalam menggambarkan karakteristik arus permukaan laut di Selat Bali. Oleh karena itu, data HF Radar digunakan untuk menggambarkan dinamika arus permukaan laut di jalur penyeberangan Selat Bali dengan pendekatan temporal pada musim timur, bulan Juni hingga Agustus 2020. Penelitian ini dilakukan selama 15 hari tiap bulan yang mencakup waktu pasang purnama dan pasang perbani. Grafik arus permukaan dibuat untuk menganalisis pola arus permukaan laut secara temporal ketika musim timur terhadap angin, pasang surut dan komponennya. Hasil menunjukkan pada musim timur, rata – rata kecepatan arus di lokasi penelitian berkisar antara 0.1 – 1.08 m/s. Arah arus permukaan dominan ke arah selatan. Karakteristik arus permukaan di Selat Bali di pengaruhi oleh pasang surut, sea level anomaly dan morfologi pantai. Secara temporal, kecepatan arus lebih besar ketika menuju surut dan ketika pasang purnama. Secara spasial, wilayah tengah perairan Selat Bali memiliki kecepatan arus yang lebih b
巴厘海峡在港口和水域有许多活动,如客运和货运、旅游和渔业。海洋条件,特别是海面洋流,影响着巴厘海峡港口和航运活动的畅通。高频雷达(HF Radar)具有高分辨率监测和绘制海流和海浪图的优势。由于海峡面积狭窄,高频雷达对描述巴厘海峡SSC的特征非常有益。因此,高频雷达数据用于描述2020年6月至8月东南季风期间,巴厘海峡SSC的动态,特别是在船舶穿越车道上。本研究每月进行15天,包括小潮和大潮。创建表面洋流图是为了分析风、潮汐及其组成部分的时间SSC模式。结果表明,在东南季风中,研究地点的平均流速在0.1–1.08 m/s之间。地表水流的方向主要是向南。巴厘海峡表面洋流的特征受到潮汐、海平面异常和海岸形态的影响。从时间上讲,退潮期和大潮时的流速更大。从空间上看,巴厘海峡中部海域水流速度较大。了解当前速度较高的区域和时间可以作为确定巴厘海峡港口之间航道的基础。巴厘岛南部的港口和水域有许多活动,如客运和货物运输、旅行和维护。海洋地理条件,特别是海面洋流,影响着巴厘岛南部的港口失事和航行活动。高频雷达(HF Radar)具有高分辨率的表面和测绘特权。由于南部海域狭窄,高频雷达在描述巴厘岛南部海面洋流特征方面非常有用。因此,高频雷达数据用于描述2020年6月至8月东部季节沿巴厘岛南部分布线的海面洋流动态。这项研究每月进行15天,包括完整的耦合时间和农场安装。制作表面流图,以临时分析东部季节逆风时的海面流模式、山脉表面和成分。结果表明,在东部季节,研究地点的平均流速在0.1–1.08 m/s之间。将主导表面指向南方。巴厘岛南部的地表流动特征受到地表、海平面异常和海岸形态的影响。暂时来说,当它变暗和完全安装时,流速会更高。从空间上看,地中海南部的流量较高。对水域和时区的了解——高速时间可以作为确定巴厘岛南部港口之间互联互通的基础。
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引用次数: 1
Hubungan Panjang Berat dan Faktor Kondisi Siput Lola (Rochia nilotica) di Perairan Maluku, Sumbawa, dan Bengkulu 影子水、松巴哇和明古鲁的重纵向关系和Lola Sip条件因子(Rochia nilotica)
Pub Date : 2022-06-20 DOI: 10.14710/jkt.v25i3.14089
Risnita Tri Utami, Putri Sapira Ibrahim, Agus Kusnadi, Dedy Kurnianto, Teddy Triandiza, Rosmi N Pesillette
Rochia nilotica (Tegulidae) is a snail that is highly important economically due to its nacre layer on their shells. Lola shells are used as raw material in nail polish and high quality pearl buttons. Recently, the production of Rochia nilotica has drastically decreased. This study aimed to investigate the relationship on length-weight and condition factors of Rochia snails from four sites. Data collection was gathered on may-September 2021 at four sites i.e Kei Island, Morellla (Central Maluku), Sumbawa Island and Enggano Island with purposive sampling method. A total 177 individuals have been collected with a detail of 35 individuals from Kei Island, 61 Individuals from Central Maluku, 51 and 29 individuals from Sumbawa Island and Enggano island respectively.  The result showed that the relationship on length-weight of Rochia snails at Enggano Island, Sumbawa Island, central Maluku and Kei Island was  W=0,00707L2,2804, 0,00686L2,3949, 0,00308L2,5478,dan 0,00025L3,0893 respectively. The result shows that the growth pattern at Bengkulu, Sumbawa, and Morella follows a negative allometric model, while those at Kei Islands follow a positive allometric. Condition factors were relatively varied 1,0020-1,0317 suggested that the research waters were still a suitable environment for the growth of top shells.   Siput lola Rochia nilotica (Tegulidae) merupakan komoditi niaga bernilai ekonomis tinggi karena cangkangnya yang memiliki lapisan mutiara. Cangkang siput lola digunakan sebagai bahan baku industri cat kuku dan kancing yang berkualitas tinggi. Akibat eksploitasi berlebihan, produksi siput lola mengalami penurunan drastis selama beberapa tahun terakhir. Penelitian ini bertujuan untuk menentukan hubungan panjang berat dan faktor kondisi dari siput lola di 4 lokasi penelitian yang dipilih. Pengambilan data dilakukan pada bulan April-September 2021 dengan menggunakan metode Purposive Sampling yang dilakukan di 4 lokasi yaitu Kepulauan Kei, Morella, Pulau Sumbawa, dan Pulau Enggano yang mewakili 3 wilayah (Timur, Tengah dan Barat) Perairan Indonesia. Siput Lola yang diperoleh sebanyak 177 individu yang berasal dari 35 individu dari Kepulauan Kei, 61 individu Morella, 51 individu Sumbawa, dan 29 individu Enggano. Hubungan panjang berat siput lola di lokasi penelitian Enggano, Sumbawa, Morella, dan Kepulauan Kei secara berurutan yaitu sebesar W=0,00707L2,2804, 0,00686L2,3949, 0,00308L2,5478,dan 0,00025L3,0893. Hasil penelitian ini menunjukkan bahwa pola pertumbuhan siput lola di Pulau Enggano, Sumbawa dan Morella mengikuti model allometrik negatif, sedangkan pola pertumbuhan siput lola di Kepulauan Kei mengikuti model allometrik positif. Faktor kondisi relatif beragam dari 1,0020-1,0317 yang mengindikasikan bahwa daerah perairan penelitian masih merupakan lingkungan yang sesuai untuk pertumbuhan siput lola.
Rochia nilotica(蜗牛科)是一种经济上非常重要的蜗牛,因为它的外壳上有珍珠层。洛拉贝壳被用作指甲油和高品质珍珠扣的原料。最近,尼罗赤酵母的产量急剧下降。本研究旨在探讨四个地点罗氏蜗牛体长重与条件因子的关系。数据收集于2021年5月至9月在四个地点收集,即Kei岛、Morellla(中马鲁古)、Sumbawa岛和Enggano岛,采用有针对性的采样方法。共收集了177只个体,其中35只来自Kei岛,61只来自中马鲁古,51只和29只分别来自Sumbawa岛和Enggano岛。结果表明,Enggano岛、Sumbawa岛、Maluku岛中部和Kei岛的Rochia蜗牛的长重关系分别为[UNK]W=0.0007L2.2804、0.00686L2.3949、0.00308L2.5478和0.00025L3.0893。结果表明,明古鲁、松巴哇和莫雷拉的生长模式遵循负异速生长模型,而凯群岛的生长模式则遵循正异速生长模式。条件因素相对不同10020-10317表明研究水域仍然是顶壳生长的合适环境。lola lola Rochia nilotica是一种具有较高经济价值的商品,因为它有珍珠层。Lola蛇壳被用作高品质指甲油行业和纽扣的先驱。由于过度开发,洛拉蛇的产量在过去几年里急剧下降。本研究旨在确定四个选定研究地点洛拉蛇的长期关系和条件因素。数据收集于2021年4月至9月期间,使用Purposive采样方法在4个地点进行,即代表印度尼西亚水域3个区域(东部、中部和西部)的Kei群岛、Morella、Sumbawa群岛和Enggano群岛。Lola的数字是来自35名Kei岛民的177人、61名Morella人、51名Sumbawa人和29名Enggano人。研究地点Enggano、Sumbawa、Morella和Kei群岛的lola蛇的纵向关系依次为W=0.00707L2.2804、0.00686L2.3949、0.00308L2.5478和0.00025L3.0893。研究结果表明,Enggano岛、Sumbawa岛和Morella岛的洛拉蛇生长模式遵循负异速生长模型,而Kei岛的洛拉蛇生长模式则遵循正异速生长模式。10020-10317年相对多样化的条件表明,研究水域仍然是适合洛拉蛇生长的环境。
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引用次数: 0
Model Prediksi Jumlah Pakan menggunakan Algoritma Evolusi Pikiran - Jaringan Syaraf Tiruan Rambatan Balik untuk Budidaya Udang 饲料数量预测模型使用了一种模拟神经进化算法——一种用于养殖虾的逆向神经网络
Pub Date : 2022-05-20 DOI: 10.14710/jkt.v25i2.14256
Erwin Adriono, Maman Somantri, C. A. Suryono
Menentukan jumlah pakan yang sesuai merupakan hal penting dalam kegiatan budidaya udang berjenis Litopenaeus Vannamei. Jumlah pakan dapat dipengaruhi oleh banyak faktor antara lain Jumlah Udang, Umur udang, DO, Salinitas, Alkalinitas, Suhu dan PH. Hubungan antar faktor tersebut dengan jumlah pakan sulit dibuatkan dalam persamaan matematis maupun dengan metode statisik. Permasalahan tersebut dapat diselesaikan menggunakan Neural network. Neural network menjadi solusi untuk memodelkan hubungan input dan output yang kompleks. Hubungan Jumlah pakan dan faktorlainnya akan dimodelkan menggunakan metode Backpropagation NN yang dikombinasikan dengan algoritma optimasi seperti Genetic Algoritm dan Mind Evotionary Algoritm. Model BPNN, BPNN – GA dan BPNN MEA akan dibandingkan performa menggunakan MSE, RSME, MAE dan MAPE. Dari ketiga metode yang digunakan didapatkan hasil paling baik adalah pada BPNN MEA yaitu nilai MSE, RSME, MAE dan MAPE berturut-turut adalah 40,92; 6,39; 6,51 dan 20,29.   Determining the appropriate amount of feed is important in the aquaculture of Litopenaeus Vannamei shrimp. The amount of feed can be influenced by many factors including the number of shrimp, shrimp age, DO, salinity, alkalinity, temperature and PH. The relationship between these factors and the amount of feed is difficult to make in mathematical equations or with statistical methods. These problems can be solved using a neural network. Neural network is a solution for modeling complex input and output relationships. The relationship between the amount of feed and other factors will be modeled using the Backpropagation NN method combined with optimization algorithms such as Genetic Algorithm and Mind Evotionary Algorithm. The BPNN, BPNN – GA and BPNN MEA models will be compared using MSE, RSME, MAE and MAPE. Of the three methods used, the best results were obtained on BPNN MEA, with values of MSE, RSME, MAE and MAPE respectively 40,92; 6,39; 6,51  and 20,29.
确定适当的饲料数量是Vannamei著名虾养殖场的一个重要领域。饲料的数量可能受到许多因素的影响,包括虾的数量、虾的年龄、DO、盐度、碱度、碱度、温度和PH。这个问题可以通过神经网络解决。神经网络成为模拟复杂的输入和输出关系的解决方案。饲料数量和其他事实将使用NN增长法与基因算法和心算算法等优化算法进行模型。BPNN、BPNN——GA和BPNN的模型将比较MSE、RSME、MAE和MAPE的表演。在这三种方法中,最有效的方法是BPNN,即MSE、RSME、MAE和MAPE连续的价值为40.92;6,39;6。51和20。29。在利奥皮纳尔乌斯(Vannamei shrimp)的水藻养殖中,确定饲料分量是重要的。喂养的数量可能会受到许多因素的影响,包括虾的数量、虾的年龄、DO、碱性、碱性、温度和PH值。这些问题可以通过神经网络解决。神经网络是一种解构输入和输出关系的解决方案。提要和其他因素之间的关系将通过乐观的方法算法和心理Evotionary算法来建模。BPNN, BPNN——GA和BPNN的模型将用MSE, RSME, MAE和MAPE来比较。在这三种不同的方法中,最好的结果是基于MSE、RSME、MAE和MAPE尊重40.92;6,39;6。51和20。29。
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引用次数: 1
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Jurnal Kelautan Tropis
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