Pub Date : 2020-09-02DOI: 10.21608/jvmr.2020.34766.1020
F. M. Halfaya, G. Ragab, U. Hagag, O. Ahmed, W. A. Elkheir
Ankle osteoarthritis (OA) is a long-standing inflammatory degeneration disease; until now, its pathogenesis remains ambiguous. There is no complete remedy from OA and the present pharmacological therapy choices are restrained and combined with undesirable side effects. Clinically, Hyaluronic acid (HA) is widely consumed to cure OA. The present experiment aimed to assess the role of HA in the remedy of experimentally Monosodium iodoacetate (MIA) -induced ankle OA in the rat model. Thirty male Wistar rats were divided into 3 groups (each of 10 rats). Rats of group I were injected with 1 mg MIA in the right ankle joint for two successive days, while those of group II were treated with saline instead of MIA; and group III (osteoarthritic + HA) rats were injected with HA in the ankle joint at 2nd, 3rd, and 4th weeks following injection of MIA. Bodyweight, ankle measurement, total leukocytescount (TLC), antioxidant response element (ARE) level, and joint Magnetic Resonance Imaging (MRI) were investigated. HA reduced expressions of joint ARE level. HA also markedly reduced the TLC. The administration of HA decreases ankle measurement in MIA-induced OA rats. MRI of HA showed a gradual reduction in joint damage. These results suggest that HA has improvement effects on OA rats which are assessed through anti-inflammatory and antioxidant effects.
{"title":"Efficacy of Hyaluronic Acid in the Treatment of MIA-induced Ankle Osteoarthritis in Rats and its Effect on Antioxidant Response Element","authors":"F. M. Halfaya, G. Ragab, U. Hagag, O. Ahmed, W. A. Elkheir","doi":"10.21608/jvmr.2020.34766.1020","DOIUrl":"https://doi.org/10.21608/jvmr.2020.34766.1020","url":null,"abstract":"Ankle osteoarthritis (OA) is a long-standing inflammatory degeneration disease; until now, its pathogenesis remains ambiguous. There is no complete remedy from OA and the present pharmacological therapy choices are restrained and combined with undesirable side effects. Clinically, Hyaluronic acid (HA) is widely consumed to cure OA. The present experiment aimed to assess the role of HA in the remedy of experimentally Monosodium iodoacetate (MIA) -induced ankle OA in the rat model. Thirty male Wistar rats were divided into 3 groups (each of 10 rats). Rats of group I were injected with 1 mg MIA in the right ankle joint for two successive days, while those of group II were treated with saline instead of MIA; and group III (osteoarthritic + HA) rats were injected with HA in the ankle joint at 2nd, 3rd, and 4th weeks following injection of MIA. Bodyweight, ankle measurement, total leukocytescount (TLC), antioxidant response element (ARE) level, and joint Magnetic Resonance Imaging (MRI) were investigated. HA reduced expressions of joint ARE level. HA also markedly reduced the TLC. The administration of HA decreases ankle measurement in MIA-induced OA rats. MRI of HA showed a gradual reduction in joint damage. These results suggest that HA has improvement effects on OA rats which are assessed through anti-inflammatory and antioxidant effects.","PeriodicalId":53046,"journal":{"name":"Journal of Veterinary Medical Research","volume":"70 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75953718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-09-02DOI: 10.21608/jvmr.2020.34762.1019
F. M. Halfaya, G. Ragab, U. Hagag, Osama M. Ahmed, W. A. Elkheir
Ankle osteoarthritis (OA) is an inflammatory deterioration chronic disease; yet, OA pathogenesis is obscure. There is no absolute cure from OA and the present pharmacological medication options are constrained and associated with adverse aspect effects. Clinically, Platelet-rich plasma (PRP) is usually used to cure OA. The present study was designed to evaluate the role of PRP in the treatment of experimentally Monosodium iodoacetate (MIA)-induced ankle osteoarthritis in the rat model. Thirty male Wistar rats were divided into three groups (each of 10 rats). Rats of group I were injected with 1 mg MIA in the right ankle joint for two consecutive days, while those of group II were treated with saline instead of MIA; and group III (osteoarthritic +PRP) rats were injected with PRP in the ankle joint at 14, 21, and 28 days after MIA injection. Paw oedema, scoring of arthritis, Matrix metalloproteinase 13 (MMP13) level, antioxidant response element (ARE) level, and joint transforming growth factor beta1 (TGF β 1) were evaluated. PRP reduces expression of joint, MMP13, ARE level, and TGF β 1. PRP also significantly reduces the score of arthritis. The administration of PRP decreases paw oedema in MIA-induced OA rats. These results suggest that PRP has marked effect against OA in MIA-induced osteoarthritic rats which are mediated through the anti-inflammatory and antioxidant effects.
{"title":"Effect of Platelet-Rich Plasma on MMP-13, ARE and TGF β1 in MIA-induced Osteoarthritis in Rats","authors":"F. M. Halfaya, G. Ragab, U. Hagag, Osama M. Ahmed, W. A. Elkheir","doi":"10.21608/jvmr.2020.34762.1019","DOIUrl":"https://doi.org/10.21608/jvmr.2020.34762.1019","url":null,"abstract":"Ankle osteoarthritis (OA) is an inflammatory deterioration chronic disease; yet, OA pathogenesis is obscure. There is no absolute cure from OA and the present pharmacological medication options are constrained and associated with adverse aspect effects. Clinically, Platelet-rich plasma (PRP) is usually used to cure OA. The present study was designed to evaluate the role of PRP in the treatment of experimentally Monosodium iodoacetate (MIA)-induced ankle osteoarthritis in the rat model. Thirty male Wistar rats were divided into three groups (each of 10 rats). Rats of group I were injected with 1 mg MIA in the right ankle joint for two consecutive days, while those of group II were treated with saline instead of MIA; and group III (osteoarthritic +PRP) rats were injected with PRP in the ankle joint at 14, 21, and 28 days after MIA injection. Paw oedema, scoring of arthritis, Matrix metalloproteinase 13 (MMP13) level, antioxidant response element (ARE) level, and joint transforming growth factor beta1 (TGF β 1) were evaluated. PRP reduces expression of joint, MMP13, ARE level, and TGF β 1. PRP also significantly reduces the score of arthritis. The administration of PRP decreases paw oedema in MIA-induced OA rats. These results suggest that PRP has marked effect against OA in MIA-induced osteoarthritic rats which are mediated through the anti-inflammatory and antioxidant effects.","PeriodicalId":53046,"journal":{"name":"Journal of Veterinary Medical Research","volume":"19 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80332829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-05-13DOI: 10.21608/jvmr.2020.88897
Safaa A. A. Abdel-Latif, Asmaa Atef, Ahmed M. A. Abdel-Aleem, Al-Hussien M . Dahshan, Ahmed Ali
In this study, 50 vaccinated broiler and one layer flock from Beni-Suef, Fayoum and Minia governorates were investigated. Necropsy lesions were suggestive of LPAI-H9N2 or NDV. Samples of tracheal swabs and organs were subjected for viral isolation and molecular characterization. Specific RT-PCR for the NDV F-gene and the HA gene of the LPAI-H9N2 viruses was used. Virus isolation and primary identification using HI test revealed 37.5 and 43.3-46.2% prevalence for LPAI-H9N2 and NDV viruses, respectively. Phylogenetic analysis of partial sequences of the F gene showed that NDV viruses belong to genotype II and VII-1.1. as indicated by the F0 protein proteolytic cleavage site motifs (aa112-117) of the NDV strains F-gene. The vNDV isolates were 98.7-99.3% and 96.6-98.9% identical to each other based on nucleotide and amino acid identities, respectively. Compared to their counterpart isolates; the lentogenic strains shared 98-99.2% and 96.3-98.1% nucleotide and amino acid identities to the LaSota reference strain. The LPAI-H9N2 phylogeny of the HA gene showed that the 2 isolates obtained in this study are related to each other and related to recent 2016-2018 Egyptian H9N2 strains. Notably, the 2 strains showed higher identity (≥99%) to recent Israeli 2018 isolates with several amino acid changes. The current study revealed wide spread of both NDV and LPAI-H9N2 viruses. The vaccine failure and the mismatch between the vaccine and circulating NDV viruses is the most probable cause of current outbreaks. The LPAI-H9N2 viruses are divergent form their ancestral viruses in Egypt indicating continuous circulation and vaccine pressure induced mutations
{"title":"Characterization of Avian Influenza H9N2 and Newcastle Disease Virus Isolated from Vaccinated Chickens in Upper Egypt","authors":"Safaa A. A. Abdel-Latif, Asmaa Atef, Ahmed M. A. Abdel-Aleem, Al-Hussien M . Dahshan, Ahmed Ali","doi":"10.21608/jvmr.2020.88897","DOIUrl":"https://doi.org/10.21608/jvmr.2020.88897","url":null,"abstract":"In this study, 50 vaccinated broiler and one layer flock from Beni-Suef, Fayoum and Minia governorates were investigated. Necropsy lesions were suggestive of LPAI-H9N2 or NDV. Samples of tracheal swabs and organs were subjected for viral isolation and molecular characterization. Specific RT-PCR for the NDV F-gene and the HA gene of the LPAI-H9N2 viruses was used. Virus isolation and primary identification using HI test revealed 37.5 and 43.3-46.2% prevalence for LPAI-H9N2 and NDV viruses, respectively. Phylogenetic analysis of partial sequences of the F gene showed that NDV viruses belong to genotype II and VII-1.1. as indicated by the F0 protein proteolytic cleavage site motifs (aa112-117) of the NDV strains F-gene. The vNDV isolates were 98.7-99.3% and 96.6-98.9% identical to each other based on nucleotide and amino acid identities, respectively. Compared to their counterpart isolates; the lentogenic strains shared 98-99.2% and 96.3-98.1% nucleotide and amino acid identities to the LaSota reference strain. The LPAI-H9N2 phylogeny of the HA gene showed that the 2 isolates obtained in this study are related to each other and related to recent 2016-2018 Egyptian H9N2 strains. Notably, the 2 strains showed higher identity (≥99%) to recent Israeli 2018 isolates with several amino acid changes. The current study revealed wide spread of both NDV and LPAI-H9N2 viruses. The vaccine failure and the mismatch between the vaccine and circulating NDV viruses is the most probable cause of current outbreaks. The LPAI-H9N2 viruses are divergent form their ancestral viruses in Egypt indicating continuous circulation and vaccine pressure induced mutations","PeriodicalId":53046,"journal":{"name":"Journal of Veterinary Medical Research","volume":"45 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81435878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-05-07DOI: 10.21608/jvmr.2020.87551
Shehata M.M.Soliman, A. H. Abdel-Razik, M. Hussein, Omima M. M. Rashad
The present study aimed to provide a detailed description of the normal development of rabbit stomach and focusing on the histogenesis of gastric glands. In a total, 24 New Zealand White rabbit fetuses were collected at gestational days 21, 25, and 29. The stomachs of the collected fetuses were fixed in 10% neutral buffered formalin and prepared by paraffin technique then stained with Harris's Haematoxylin and Eosin, Masson's Trichrome stain, Orcein, Periodic acid-Schiff, Alcian blue, and Bromophenol blue stains. The results revealed that, at 21stgestational day, the different parts of the stomach including, cardia, fundus and pylorus could be easily distinguished. On 25th developmental day, the gastric mucosal folds were more prominent in the cardia than fundus and pylorus. At 29th developmental day, tunica mucosa and tunica submucosa of the fetal stomach were laid in longitudinally oriented folds known as rugae. The gastric gland in this age became well developed containing well-demarcated oxyntic and peptic cells. In conclusion, the rabbit stomach is completely differentiated during the embryonic life and the gastric glands were functionally active.
{"title":"Histological and Histochemical investigation of the development of the New -Zealand rabbit’s gastric glands","authors":"Shehata M.M.Soliman, A. H. Abdel-Razik, M. Hussein, Omima M. M. Rashad","doi":"10.21608/jvmr.2020.87551","DOIUrl":"https://doi.org/10.21608/jvmr.2020.87551","url":null,"abstract":"The present study aimed to provide a detailed description of the normal development of rabbit stomach and focusing on the histogenesis of gastric glands. In a total, 24 New Zealand White rabbit fetuses were collected at gestational days 21, 25, and 29. The stomachs of the collected fetuses were fixed in 10% neutral buffered formalin and prepared by paraffin technique then stained with Harris's Haematoxylin and Eosin, Masson's Trichrome stain, Orcein, Periodic acid-Schiff, Alcian blue, and Bromophenol blue stains. The results revealed that, at 21stgestational day, the different parts of the stomach including, cardia, fundus and pylorus could be easily distinguished. On 25th developmental day, the gastric mucosal folds were more prominent in the cardia than fundus and pylorus. At 29th developmental day, tunica mucosa and tunica submucosa of the fetal stomach were laid in longitudinally oriented folds known as rugae. The gastric gland in this age became well developed containing well-demarcated oxyntic and peptic cells. In conclusion, the rabbit stomach is completely differentiated during the embryonic life and the gastric glands were functionally active.","PeriodicalId":53046,"journal":{"name":"Journal of Veterinary Medical Research","volume":"22 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141206179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-02-01DOI: 10.21608/jvmr.2020.69693
Kotb, M. M. A, R. G. A., Fathy, M. Z ., Haggag, U., Nesreen. M. Safwat
{"title":"Radiologic, Ultrasonic and pathological assessments of locally applied estrogen on promotion of experimentally induced tibial fracture healing in rats","authors":"Kotb, M. M. A, R. G. A., Fathy, M. Z ., Haggag, U., Nesreen. M. Safwat","doi":"10.21608/jvmr.2020.69693","DOIUrl":"https://doi.org/10.21608/jvmr.2020.69693","url":null,"abstract":"","PeriodicalId":53046,"journal":{"name":"Journal of Veterinary Medical Research","volume":"21 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73678903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-08-05DOI: 10.21608/JVMR.2019.66110
Kamel M A Hassanin, S. Ibrahim, A. Abdel-Wahab, Dina M M H El-Kossi, A. H. Abdel-Razik
The current study aimed to investigate the effects of prenatal melatonin “MLT” administration against bisphenol A “BPA”- induced infertility and thyroid dysfunction in male rat offsprings (First generation “F1”). For that purpose, fifty adult albino rats (40 females and 10 males) were used and classified equally into five groups (8 females and 2 males in each group). First group (control group) in which, pregnant rats were injected with 0.3 ml of vehicle /day. The second group (low dose BPA) where rats received a daily dose of 25 µg / kg B.W. The third group (high dose BPA) where rats received a daily dose of 250 µg / kg B.W. Fourth group (low dose BPA + MLT) where rats received a daily dose of 25 µg BPA /kg B.W. plus 10 mg MLT / Kg B.W. The fifth group (high dose BPA + MLT) where the rats received a daily dose of 250 µg BPA / kg B.W. plus 10 mg /Kg B.W. All rats within each group received their specific treatment daily with subcutaneous injection starting from the fourth day of pregnancy till full term. Then, the male offsprings of each group were selected and reared until the 60th day after birth. Serum and tissue samples were collected for analyses and microscopical examination. Although prenatal administration of both BPA doses didn’t affect the body weight gain and testicular weights of male offsprings, they reduced significantly the serum levels of testosterone and triodotyrosine when compared to the control group. Also, both BPA doses disturb significantly the oxidant/antioxidant ratio. Moreover, prenatal administration of both BPA doses affected negatively semen quality of the produced offsprings and induced marked histological alterations in their testes and prostate. Remarkably, all serological and histological alterations observed after BPA exposure were ameliorated significantly with MLT co-administration. Thus, prenatal MLT administration could be considered an optimal treatment to relieve many reproductive disorders, degenerative changes of testes and prostate and thyroid malfunction induced in male offsprings after gestational exposure of their dams to BPA.
{"title":"Rescue effects of prenatal melatonin administration against bisphenol A- induced perturbations of reproductive and thyroid activities in male rat offsprings","authors":"Kamel M A Hassanin, S. Ibrahim, A. Abdel-Wahab, Dina M M H El-Kossi, A. H. Abdel-Razik","doi":"10.21608/JVMR.2019.66110","DOIUrl":"https://doi.org/10.21608/JVMR.2019.66110","url":null,"abstract":"The current study aimed to investigate the effects of prenatal melatonin “MLT” administration against bisphenol A “BPA”- induced infertility and thyroid dysfunction in male rat offsprings (First generation “F1”). For that purpose, fifty adult albino rats (40 females and 10 males) were used and classified equally into five groups (8 females and 2 males in each group). First group (control group) in which, pregnant rats were injected with 0.3 ml of vehicle /day. The second group (low dose BPA) where rats received a daily dose of 25 µg / kg B.W. The third group (high dose BPA) where rats received a daily dose of 250 µg / kg B.W. Fourth group (low dose BPA + MLT) where rats received a daily dose of 25 µg BPA /kg B.W. plus 10 mg MLT / Kg B.W. The fifth group (high dose BPA + MLT) where the rats received a daily dose of 250 µg BPA / kg B.W. plus 10 mg /Kg B.W. All rats within each group received their specific treatment daily with subcutaneous injection starting from the fourth day of pregnancy till full term. Then, the male offsprings of each group were selected and reared until the 60th day after birth. Serum and tissue samples were collected for analyses and microscopical examination. Although prenatal administration of both BPA doses didn’t affect the body weight gain and testicular weights of male offsprings, they reduced significantly the serum levels of testosterone and triodotyrosine when compared to the control group. Also, both BPA doses disturb significantly the oxidant/antioxidant ratio. Moreover, prenatal administration of both BPA doses affected negatively semen quality of the produced offsprings and induced marked histological alterations in their testes and prostate. Remarkably, all serological and histological alterations observed after BPA exposure were ameliorated significantly with MLT co-administration. Thus, prenatal MLT administration could be considered an optimal treatment to relieve many reproductive disorders, degenerative changes of testes and prostate and thyroid malfunction induced in male offsprings after gestational exposure of their dams to BPA.","PeriodicalId":53046,"journal":{"name":"Journal of Veterinary Medical Research","volume":"14 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74370161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-06-01DOI: 10.21608/JVMR.2019.43335
E. F.R., A. A.H., H. H.M., Nabih A.M., E. Khalifa, S. S.E.
Pneumonic pasteurellosis is the main cause of severe respiratory tract infections incalves and causing great economic losses. The objective of this research was to studythe antimicrobial susceptibility of P. multocida and M. haemolytica and detection theeffect of Lysozyme and Nitric oxide; as immune parameters, on most importantbacteria causing pneumonic pasteurellosis in cattle calves. A total number of 406 deepnasal swabs and blood samples were collected from 406 bovine calves suffered fromrespiratory manifestations. Bacteriological examination revealed that the overallprevalence of both P. multocida and M. haemolytica was of 26.6%; 18.2% for P.multocida and 8.4%for M. haemolytica. P. multocida was singly isolated from 4.9% ofcases. While it was mixed with S. aureus, E. coli, Streptococcus spp., both S. aureus andE. coli, both S. aureus and Streptococcus spp. and both E. coli and Streptococcus spp.with percentages of 4%, 1.2%, 2.2%, 1.7%, 3.2% and 1.0%, respectively. Meanwhile,M. haemolytica was isolated as a single isolate from 1.7% of cases while it was mixedwith S. aureus, Streptococcus spp., both S. aureus and Streptococcus spp. and both E.coli and Streptococcus spp. with percentages of 2.7%, 1.2%, 2.5% and 0.2%,respectively. The in in-vitro sensitivity testing of all isolates showed high susceptibilityto Fluoroquinolones and cephalosporins. On the other hand, high resistances wereobtained against tetracyclines, penicillins and aminoglycosides. On the immunologicallevel, the data of the existing research show that all respiratory affected calves recordsignificant elevation of nitric oxide level in compare with normal control calves.However, all infected calves elucidate significant reduction of lysozyme activity.
{"title":"Antimicrobial and immunological studies on Pasteurella multocida and Mannheimia haemolytica recovered from calves affected with respiratory manifestations","authors":"E. F.R., A. A.H., H. H.M., Nabih A.M., E. Khalifa, S. S.E.","doi":"10.21608/JVMR.2019.43335","DOIUrl":"https://doi.org/10.21608/JVMR.2019.43335","url":null,"abstract":"Pneumonic pasteurellosis is the main cause of severe respiratory tract infections incalves and causing great economic losses. The objective of this research was to studythe antimicrobial susceptibility of P. multocida and M. haemolytica and detection theeffect of Lysozyme and Nitric oxide; as immune parameters, on most importantbacteria causing pneumonic pasteurellosis in cattle calves. A total number of 406 deepnasal swabs and blood samples were collected from 406 bovine calves suffered fromrespiratory manifestations. Bacteriological examination revealed that the overallprevalence of both P. multocida and M. haemolytica was of 26.6%; 18.2% for P.multocida and 8.4%for M. haemolytica. P. multocida was singly isolated from 4.9% ofcases. While it was mixed with S. aureus, E. coli, Streptococcus spp., both S. aureus andE. coli, both S. aureus and Streptococcus spp. and both E. coli and Streptococcus spp.with percentages of 4%, 1.2%, 2.2%, 1.7%, 3.2% and 1.0%, respectively. Meanwhile,M. haemolytica was isolated as a single isolate from 1.7% of cases while it was mixedwith S. aureus, Streptococcus spp., both S. aureus and Streptococcus spp. and both E.coli and Streptococcus spp. with percentages of 2.7%, 1.2%, 2.5% and 0.2%,respectively. The in in-vitro sensitivity testing of all isolates showed high susceptibilityto Fluoroquinolones and cephalosporins. On the other hand, high resistances wereobtained against tetracyclines, penicillins and aminoglycosides. On the immunologicallevel, the data of the existing research show that all respiratory affected calves recordsignificant elevation of nitric oxide level in compare with normal control calves.However, all infected calves elucidate significant reduction of lysozyme activity.","PeriodicalId":53046,"journal":{"name":"Journal of Veterinary Medical Research","volume":"96 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86606275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-06-01DOI: 10.21608/JVMR.2019.43345
F. El-Seedy, Aml Mokhtar, E. Khalifa, S. Sayed
Bacterial disease still has serious problem in the intensive poultry production. In the recent years, particular concern has been raised by high incidence of poultry infections by E. coli. Analyses of antibacterial properties of essential oils have been carried out by range of researches. This experiment aimed to study the effect of immunomodulators on the immunogenicity of vaccine E. coli O78. In this study 250 broiler chickens were used. They were divided into 5 separated groups all groups vaccinated with E. coli O78 cebel coarse spray vaccine, except control group, 2 groups treated with immunomodulators, 3 groups challenged with untyped E. coli strain, all chickens housed in separated anavar. First group was control, 2nd group was vaccinated only, 3rd group was vaccinated and challenged, 4th group was vaccinated and received immunomodulators and 5th group was vaccinated, received immunomodulators and challenged. All chickens were observed daily food consumption, weight gained mortality rate, lesion, bioavailability, and weekly collected blood samples from 2-5 birds. The results were summarized as follows; immunomodulators have positive effect on B.W.G, decreased mortality and morbidity rate. The challenge enhanced the effect of E. coli O78 vaccine and there was marked improvement in bioavailability, B.W.G and immune defense against bacterial and respiratory diseases. Also, immunomodulators increased immunogenicity against bacterial disease through enhancing immune response system, and had synergistic effect with vaccination against E. coli.
{"title":"Factors affecting the immunogenicity of E. coli O78 vaccine in chickens","authors":"F. El-Seedy, Aml Mokhtar, E. Khalifa, S. Sayed","doi":"10.21608/JVMR.2019.43345","DOIUrl":"https://doi.org/10.21608/JVMR.2019.43345","url":null,"abstract":"Bacterial disease still has serious problem in the intensive poultry production. In the recent years, particular concern has been raised by high incidence of poultry infections by E. coli. Analyses of antibacterial properties of essential oils have been carried out by range of researches. This experiment aimed to study the effect of immunomodulators on the immunogenicity of vaccine E. coli O78. In this study 250 broiler chickens were used. They were divided into 5 separated groups all groups vaccinated with E. coli O78 cebel coarse spray vaccine, except control group, 2 groups treated with immunomodulators, 3 groups challenged with untyped E. coli strain, all chickens housed in separated anavar. First group was control, 2nd group was vaccinated only, 3rd group was vaccinated and challenged, 4th group was vaccinated and received immunomodulators and 5th group was vaccinated, received immunomodulators and challenged. All chickens were observed daily food consumption, weight gained mortality rate, lesion, bioavailability, and weekly collected blood samples from 2-5 birds. The results were summarized as follows; immunomodulators have positive effect on B.W.G, decreased mortality and morbidity rate. The challenge enhanced the effect of E. coli O78 vaccine and there was marked improvement in bioavailability, B.W.G and immune defense against bacterial and respiratory diseases. Also, immunomodulators increased immunogenicity against bacterial disease through enhancing immune response system, and had synergistic effect with vaccination against E. coli.","PeriodicalId":53046,"journal":{"name":"Journal of Veterinary Medical Research","volume":"22 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89706406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-06-01DOI: 10.21608/JVMR.2019.43342
Khalafalla F.A, S Abdel-AttyN., Omima I.Ali
Pesticides are used extensively especially in developing countries like Egypt to control pest either in animal or in agriculture, which may lead to harmful residues in foods of animal origin. The current study was conducted to estimate the residue level of OC and pyrethroid in 320 beef and sheep samples (160each) collected from different shops at Beni-Suef governorate during summer and winter season. The collected samples were liver, muscle, kidney, and fat (80 each; 4o from each animal species).Among fourteen organochlorine compound examined, only Alpha HCH was detected in samples of cattle and sheep collected through winter season in a level below the MRL, while through summer season, only Alpha HCH and Delta HCH were detected in sheep samples in a level below the MRL. Pyrethroid pesticides residues represented by cypermethrin, deltamethrin, Esfenvalerate, permethrin were not detected through winter season, while they were detected in muscles of cattle and fat of sheep through summer season, while Labdacyhalothrin, bifenthrin, cyfluthrin, Meothrin were detected in most of examined samples from different species through winter and summer seasons, most of these results revealed higher mean level than the maximum residue limits. From these results most of OC could not be detected may be due to these compounds not used science 1970, on other hand pyrethroid it still used nowadays in Egypt either in agriculture or as spray in animals to control ectoparasites spatially in summer season.
{"title":"Pesticides residues in retail meat and offal","authors":"Khalafalla F.A, S Abdel-AttyN., Omima I.Ali","doi":"10.21608/JVMR.2019.43342","DOIUrl":"https://doi.org/10.21608/JVMR.2019.43342","url":null,"abstract":"Pesticides are used extensively especially in developing countries like Egypt to control pest either in animal or in agriculture, which may lead to harmful residues in foods of animal origin. The current study was conducted to estimate the residue level of OC and pyrethroid in 320 beef and sheep samples (160each) collected from different shops at Beni-Suef governorate during summer and winter season. The collected samples were liver, muscle, kidney, and fat (80 each; 4o from each animal species).Among fourteen organochlorine compound examined, only Alpha HCH was detected in samples of cattle and sheep collected through winter season in a level below the MRL, while through summer season, only Alpha HCH and Delta HCH were detected in sheep samples in a level below the MRL. Pyrethroid pesticides residues represented by cypermethrin, deltamethrin, Esfenvalerate, permethrin were not detected through winter season, while they were detected in muscles of cattle and fat of sheep through summer season, while Labdacyhalothrin, bifenthrin, cyfluthrin, Meothrin were detected in most of examined samples from different species through winter and summer seasons, most of these results revealed higher mean level than the maximum residue limits. From these results most of OC could not be detected may be due to these compounds not used science 1970, on other hand pyrethroid it still used nowadays in Egypt either in agriculture or as spray in animals to control ectoparasites spatially in summer season.","PeriodicalId":53046,"journal":{"name":"Journal of Veterinary Medical Research","volume":"48 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74362280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-06-01DOI: 10.21608/JVMR.2019.43328
Asmaa Abd Elatiff, A. A. El-Sawah, M. M. Amer, Al-Hussien M . Dahshan, H. Salam, Salama A. S. Shany
Commercial broiler chickens are frequently infected with Ecoli serotypes in form of collibacillosis whichcharacterized as an initial respiratory infection (air sacculitis) followed by generalized septicemia, perihepatitis andpericarditis. The present work aimed to study the ability of E.coli O125 previuosly isolated and identified fromdiseased broilers suffered from pericarditis , airsacculitis and perihapatitis to induce a disease and The current studyaimed to determine the pathogenicity of E. coli serogroup O125 in broiler chicks, a trial of prevention and treatmentusing probiotic ( Guardyazin-m) or antibiotic (Cipronate20%). The chicks were divided into seven groups includingcontrol negative, orally infected , subcutenously infected , probiotic treated group, and finally antibiotic treatedgroup. The clinical signs, mortality, performance parameters and postmortem examination as well as thepathological changes in liver and intestine were recorded. The probiotic group gave the best results in controllinginfection by E. coli O125 followed by antibiotic. In conclusion, the E. coli O125 serogroup was pathogenic to chickswhen experimentally inoculated, so particular attention must be directed toward E. coli O125 as a pathogen infectingbird. Also probiotics was of great value in protection against the E. coli infection and improve the performanceparameters of chicks . Its effect on feed consumption , weekly body weight gain and feed conversion rate (FCR) wasdetermined.
{"title":"Pathogenicity of Escherichia coli O125 in commercial broiler chickens","authors":"Asmaa Abd Elatiff, A. A. El-Sawah, M. M. Amer, Al-Hussien M . Dahshan, H. Salam, Salama A. S. Shany","doi":"10.21608/JVMR.2019.43328","DOIUrl":"https://doi.org/10.21608/JVMR.2019.43328","url":null,"abstract":"Commercial broiler chickens are frequently infected with Ecoli serotypes in form of collibacillosis whichcharacterized as an initial respiratory infection (air sacculitis) followed by generalized septicemia, perihepatitis andpericarditis. The present work aimed to study the ability of E.coli O125 previuosly isolated and identified fromdiseased broilers suffered from pericarditis , airsacculitis and perihapatitis to induce a disease and The current studyaimed to determine the pathogenicity of E. coli serogroup O125 in broiler chicks, a trial of prevention and treatmentusing probiotic ( Guardyazin-m) or antibiotic (Cipronate20%). The chicks were divided into seven groups includingcontrol negative, orally infected , subcutenously infected , probiotic treated group, and finally antibiotic treatedgroup. The clinical signs, mortality, performance parameters and postmortem examination as well as thepathological changes in liver and intestine were recorded. The probiotic group gave the best results in controllinginfection by E. coli O125 followed by antibiotic. In conclusion, the E. coli O125 serogroup was pathogenic to chickswhen experimentally inoculated, so particular attention must be directed toward E. coli O125 as a pathogen infectingbird. Also probiotics was of great value in protection against the E. coli infection and improve the performanceparameters of chicks . Its effect on feed consumption , weekly body weight gain and feed conversion rate (FCR) wasdetermined.","PeriodicalId":53046,"journal":{"name":"Journal of Veterinary Medical Research","volume":"134 7-8","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72493142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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