Pub Date : 2019-06-01DOI: 10.21608/JVMR.2019.43346
I. Radwan, S. Tamam, Abeer A. El Sayed, Rania O. Qurani
E. coli infections in avian species are an economic threat to the poultry industry worldwide. The spread of MDR bacteria has been recognized as an increasing problem in the veterinary and medical fields. The current study aimed to investigate the phenotypic and genotypic antimicrobial patterns of avian Pathogenic E. coli isolated from broiler chickens. Results of in-vitro antimicrobial susceptibility testing showed that E. coli isolates were more sensitive to imipenem only (72.4%). On the other hand, they were highly resistant to most of used antimicrobials including ciprofloxacin (95.9%), amikacin (94.9%), cefotaxime sodium (92.9%), gentamicin (89.9%), cefotriaxone (89.9%), topramycin (87.8%), sulphamethoxazole/trimethoprime (85.7%), ceftazidim (84.7%). Also, they were resistant to cefoprazone (79.9%), doxycycline (72.4%) and amoxycillin/clavulinic acid (69.4%). All the tested isolates of E. coli (100%) were multi drug resistant (MDR). PCR was applied on 15 MDR E. coli isolates to determine 4 genes responsible for antibiotic resistance included ampC, blaCTX, blaSHV and tetA (A). The results revealed that that ampC and blaCTX genes were the most prevalent found in all isolates (100%) while tetA (A) and blaSHV genes were harbored in 14 isolates (93.3%).
{"title":"Antimicrobial patterns of Avian Pathogenic Escherichia coli isolated from broiler chickens","authors":"I. Radwan, S. Tamam, Abeer A. El Sayed, Rania O. Qurani","doi":"10.21608/JVMR.2019.43346","DOIUrl":"https://doi.org/10.21608/JVMR.2019.43346","url":null,"abstract":"E. coli infections in avian species are an economic threat to the poultry industry worldwide. The spread of MDR bacteria has been recognized as an increasing problem in the veterinary and medical fields. The current study aimed to investigate the phenotypic and genotypic antimicrobial patterns of avian Pathogenic E. coli isolated from broiler chickens. Results of in-vitro antimicrobial susceptibility testing showed that E. coli isolates were more sensitive to imipenem only (72.4%). On the other hand, they were highly resistant to most of used antimicrobials including ciprofloxacin (95.9%), amikacin (94.9%), cefotaxime sodium (92.9%), gentamicin (89.9%), cefotriaxone (89.9%), topramycin (87.8%), sulphamethoxazole/trimethoprime (85.7%), ceftazidim (84.7%). Also, they were resistant to cefoprazone (79.9%), doxycycline (72.4%) and amoxycillin/clavulinic acid (69.4%). All the tested isolates of E. coli (100%) were multi drug resistant (MDR). PCR was applied on 15 MDR E. coli isolates to determine 4 genes responsible for antibiotic resistance included ampC, blaCTX, blaSHV and tetA (A). The results revealed that that ampC and blaCTX genes were the most prevalent found in all isolates (100%) while tetA (A) and blaSHV genes were harbored in 14 isolates (93.3%).","PeriodicalId":53046,"journal":{"name":"Journal of Veterinary Medical Research","volume":"8 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89636577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-06-01DOI: 10.21608/JVMR.2019.43329
Mabrouk E.A., A. K.M., N. Helmy, Rehab M. Reda
h: 0px; "> The present study aimed to determine the effect of adding plant oils; extra virgin olive oil (EVOO), sunflower and soybean to animal feed on serum estradiol (E2) and progesterone (P4) levels, histological structure of ovaries and in vitro maturation of oocytes (IVM). A total of 60 mature female Albino rats were used. Animals were divided equally into 5 groups; control group (received standard diet), group II (received EVOO), group III (received sunflower oil), group IV (received soybean oil) and group VI (received oil mixture which consist of sunflower and soybean oils). After 6 weeks of feeding oil addited diet, blood samples were collected from all rats throughout the different stages of estrous cycle. Sera were used for determination of serum E2 and P4 levels. Only females that were not in estrus were scarified after the last blood sample collection, ovaries were harvested for histopathological examination and for in vitro maturation. Results showed that none of oils led to ovarian changes except soybean oil and oil mixture, cause congestion of some ovarian blood vessels. It wasalso noted that the hormonal pattern didn’t differ significantly among different treatments within the same stage of the cycle, except for the group received oil mixture where E2 and P4 levels decreased significantly (P < 0.05) during metestrus and diestrus phases, respectively. In the treated groups, the highest significant (P < 0.05) oocyte recovery rate (RR) (5.43 ± 0.23% and 4.41± 0.13%) and maturation rate (MR) (79.17 ± 2.03% and 73.43 ± 1.97%) were attained after application of EVOO followed by sunflower oil, respectively. While the lowest values were calculated with the soybean oil and oil mixture (3.83 ± 0.13 % and 2.50 ± 0.16 %) and (68.18 ± 2.29 % and 62.50 ± 2.23 %), respectively. It could be concluded that EVOO as well as sunflower oil have a beneficial influence on ovarian functional performance, retrieval of high number of good quality oocytes and raise oocyte maturation.
{"title":"EFFECT OF SOME PLANT OILS ON REPRODUCTIVE ACTIVITIES IN FEMALE ALBINO RATS","authors":"Mabrouk E.A., A. K.M., N. Helmy, Rehab M. Reda","doi":"10.21608/JVMR.2019.43329","DOIUrl":"https://doi.org/10.21608/JVMR.2019.43329","url":null,"abstract":"h: 0px; \"> The present study aimed to determine the effect of adding plant oils; extra virgin olive oil (EVOO), sunflower and soybean to animal feed on serum estradiol (E2) and progesterone (P4) levels, histological structure of ovaries and in vitro maturation of oocytes (IVM). A total of 60 mature female Albino rats were used. Animals were divided equally into 5 groups; control group (received standard diet), group II (received EVOO), group III (received sunflower oil), group IV (received soybean oil) and group VI (received oil mixture which consist of sunflower and soybean oils). After 6 weeks of feeding oil addited diet, blood samples were collected from all rats throughout the different stages of estrous cycle. Sera were used for determination of serum E2 and P4 levels. Only females that were not in estrus were scarified after the last blood sample collection, ovaries were harvested for histopathological examination and for in vitro maturation. Results showed that none of oils led to ovarian changes except soybean oil and oil mixture, cause congestion of some ovarian blood vessels. It wasalso noted that the hormonal pattern didn’t differ significantly among different treatments within the same stage of the cycle, except for the group received oil mixture where E2 and P4 levels decreased significantly (P < 0.05) during metestrus and diestrus phases, respectively. In the treated groups, the highest significant (P < 0.05) oocyte recovery rate (RR) (5.43 ± 0.23% and 4.41± 0.13%) and maturation rate (MR) (79.17 ± 2.03% and 73.43 ± 1.97%) were attained after application of EVOO followed by sunflower oil, respectively. While the lowest values were calculated with the soybean oil and oil mixture (3.83 ± 0.13 % and 2.50 ± 0.16 %) and (68.18 ± 2.29 % and 62.50 ± 2.23 %), respectively. It could be concluded that EVOO as well as sunflower oil have a beneficial influence on ovarian functional performance, retrieval of high number of good quality oocytes and raise oocyte maturation.","PeriodicalId":53046,"journal":{"name":"Journal of Veterinary Medical Research","volume":"5 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77584400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-06-01DOI: 10.21608/JVMR.2019.43347
I. Radwan, S. Tamam, Abeer A. El Sayed, Rania O. Qurani
Colibacillosis is one of the most important diseases of chickens, resulting in significant losses. The current study aimed to investigate the prevalence of E. coli infections in broiler chickens detecting their phenotypic characters such as Congo red binding activity, serum resistance and proteolytic activities. Samples were collected from 297 broiler chickens of different ages from different farms in El-Fayoum Governorate during the period from April 2017 up to March 2018. Bacteriological examination showed that 98 E. coli isolates were recovered with a prevalence rate of 33%. Results of Congo red binding activity and serum resistance revealed that all E. coli isolates (100%) showed Congo red binding activity and survive for 1 and 6 hrs and grown for 18 hrs in the presence of serum. Results of proteolytic activity revealed that 43 E. coli isolates (43.9%) were able to digest casein of skimmed milk while 16 isolates (16.3%) were positive for gelatin liquefaction test.
{"title":"Phenotypic characterization of Escherichia coli isolated from broiler chickens","authors":"I. Radwan, S. Tamam, Abeer A. El Sayed, Rania O. Qurani","doi":"10.21608/JVMR.2019.43347","DOIUrl":"https://doi.org/10.21608/JVMR.2019.43347","url":null,"abstract":"Colibacillosis is one of the most important diseases of chickens, resulting in significant losses. The current study aimed to investigate the prevalence of E. coli infections in broiler chickens detecting their phenotypic characters such as Congo red binding activity, serum resistance and proteolytic activities. Samples were collected from 297 broiler chickens of different ages from different farms in El-Fayoum Governorate during the period from April 2017 up to March 2018. Bacteriological examination showed that 98 E. coli isolates were recovered with a prevalence rate of 33%. Results of Congo red binding activity and serum resistance revealed that all E. coli isolates (100%) showed Congo red binding activity and survive for 1 and 6 hrs and grown for 18 hrs in the presence of serum. Results of proteolytic activity revealed that 43 E. coli isolates (43.9%) were able to digest casein of skimmed milk while 16 isolates (16.3%) were positive for gelatin liquefaction test.","PeriodicalId":53046,"journal":{"name":"Journal of Veterinary Medical Research","volume":"2 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89028569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-06-01DOI: 10.21608/JVMR.2019.43337
Radwan Ia, K. M.F., H. .D.A, Mahmoud .Z.A
A total of 225 samples were collected from suspected cases and they were 75 human samples (40 ear swabs, 29 vaginal swabs and 6 sputum samples) and 150 from broiler chickens for microbiological examination and fungal isolation, they collected from different areas in El-Fayoum and BeniSuef governorates, from which 129 fungal isolates were recovered, 22 fungal isolates( 29.3%) were recovered from human; of which 15 isolates of ear swabs ( A.fumigatus 37.5%) while 7 (24.1%) A.fumigatus isolates were recovered from women, but there was no A.fumigatus recovered from sputum samples, as well as there were 53 A.fumigatus isolates (35.3%) recovered from broiler chicken. The antifungal activities of thymol and carvacrol oils against the recovered fungal isolates were tested using agar dilution method. Thymol and carvacrol oils completely inhibited the growth of different fungal isolates at concentrations of 1% and 0.1%. On the other hand, the concentration of 0.01% was too weak to inhibit the fungal growth, but it completely reduced the colour of the fungal colony converted it into white coloured arial mycelium. PCR assay using oligonucleotide primer that amplifying 250bp fragment in its Gene of A.fumigatus and A.niger was performed. Sequence analysis of two isolates of A.niger and A.fumigatus using its Gene was performed.
{"title":"Correlation between Aspergillus fumigatus isolates recovered from human and broiler chickens","authors":"Radwan Ia, K. M.F., H. .D.A, Mahmoud .Z.A","doi":"10.21608/JVMR.2019.43337","DOIUrl":"https://doi.org/10.21608/JVMR.2019.43337","url":null,"abstract":"A total of 225 samples were collected from suspected cases and they were 75 human samples (40 ear swabs, 29 vaginal swabs and 6 sputum samples) and 150 from broiler chickens for microbiological examination and fungal isolation, they collected from different areas in El-Fayoum and BeniSuef governorates, from which 129 fungal isolates were recovered, 22 fungal isolates( 29.3%) were recovered from human; of which 15 isolates of ear swabs ( A.fumigatus 37.5%) while 7 (24.1%) A.fumigatus isolates were recovered from women, but there was no A.fumigatus recovered from sputum samples, as well as there were 53 A.fumigatus isolates (35.3%) recovered from broiler chicken. The antifungal activities of thymol and carvacrol oils against the recovered fungal isolates were tested using agar dilution method. Thymol and carvacrol oils completely inhibited the growth of different fungal isolates at concentrations of 1% and 0.1%. On the other hand, the concentration of 0.01% was too weak to inhibit the fungal growth, but it completely reduced the colour of the fungal colony converted it into white coloured arial mycelium. PCR assay using oligonucleotide primer that amplifying 250bp fragment in its Gene of A.fumigatus and A.niger was performed. Sequence analysis of two isolates of A.niger and A.fumigatus using its Gene was performed.","PeriodicalId":53046,"journal":{"name":"Journal of Veterinary Medical Research","volume":"20 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85489280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-06-01DOI: 10.21608/JVMR.2019.43333
Asmaa Abd Elatiff, A. A. El-Sawah, M. M. Amer, Al-Hussien M . Dahshan, Salama A. S. Shany
E coli infection in poultry is one of the principle causes of mortality and morbidity in chickens and turkeys resulting in retardationof growth and decreased feed conversion rate . The most common form of collibacillosis is characterized as an initial respiratoryinfection (air sacculitis) followed by generalized septicemia, perihepatitis and pericarditis. The present study aimed to identify thebacteria associated with pericarditis , airsacculitis and perihapatitis in Egyptian broiler chickens. A total 300 samples of diseasedand dead broilers from 3-6 weeks age were collected. The isolated bacteria included E. coli, Klebsiella spp., Shigella spp. AndEnterobacter spp. Sero-grouping of the isolated E. coli strains revealed O125, O158, O55,O129, O20 , O6 , O8 , O27, O115,O142 and un-typed strains with prevalence of 16%, 12%, 8%, 8%, 8% , 8% , 8% , 8% ,4%,4% and 16% respectively. Themajority of E coli isolates were sensitive to colistin sulphate (38%) and Norofloxacin (38%) followed by ciprofloxacin (19%),cefotaxim (19%) and Ofloxacin (19%). On the other hand, E coli were resistant (100%)to amoxicillin, clindamycin ,Erythromycin and streptomycin. PCR analysis for antibiotic resistance genes of E coli detected that 12 serogroups isolates werepositive using the specific primers for Aada2 , BlaCTX and TetA(A) genes. The current study demonstrated the high prevalenceof E. coli indeed broilers suffered from pericarditis , perihepatitis and airsacculitis . Measures are needed to control E. colicontamination in poultry farms to reduce economic losses caused by infection of E.coli.
{"title":"Serogrouping and resistance gene detection in avian pathogenic E.coli isolated from broiler chickens","authors":"Asmaa Abd Elatiff, A. A. El-Sawah, M. M. Amer, Al-Hussien M . Dahshan, Salama A. S. Shany","doi":"10.21608/JVMR.2019.43333","DOIUrl":"https://doi.org/10.21608/JVMR.2019.43333","url":null,"abstract":"E coli infection in poultry is one of the principle causes of mortality and morbidity in chickens and turkeys resulting in retardationof growth and decreased feed conversion rate . The most common form of collibacillosis is characterized as an initial respiratoryinfection (air sacculitis) followed by generalized septicemia, perihepatitis and pericarditis. The present study aimed to identify thebacteria associated with pericarditis , airsacculitis and perihapatitis in Egyptian broiler chickens. A total 300 samples of diseasedand dead broilers from 3-6 weeks age were collected. The isolated bacteria included E. coli, Klebsiella spp., Shigella spp. AndEnterobacter spp. Sero-grouping of the isolated E. coli strains revealed O125, O158, O55,O129, O20 , O6 , O8 , O27, O115,O142 and un-typed strains with prevalence of 16%, 12%, 8%, 8%, 8% , 8% , 8% , 8% ,4%,4% and 16% respectively. Themajority of E coli isolates were sensitive to colistin sulphate (38%) and Norofloxacin (38%) followed by ciprofloxacin (19%),cefotaxim (19%) and Ofloxacin (19%). On the other hand, E coli were resistant (100%)to amoxicillin, clindamycin ,Erythromycin and streptomycin. PCR analysis for antibiotic resistance genes of E coli detected that 12 serogroups isolates werepositive using the specific primers for Aada2 , BlaCTX and TetA(A) genes. The current study demonstrated the high prevalenceof E. coli indeed broilers suffered from pericarditis , perihepatitis and airsacculitis . Measures are needed to control E. colicontamination in poultry farms to reduce economic losses caused by infection of E.coli.","PeriodicalId":53046,"journal":{"name":"Journal of Veterinary Medical Research","volume":"455 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82942746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-06-01DOI: 10.21608/JVMR.2019.43332
A. Farahat, A. SawiressF., A. AghwiderA.
The aim of the present study is to investigate the effect of virgin olive oil on some blood parameters in male Albino rats. Thirty male Sprague Dawley rats, (90-110 g), were used in the present study and were divided into three groups (10 in each), 1st group (control), received basal diet and supplemented with 1ml saline. 2nd and 3rd groups received basal diet, and supplemented daily with 1ml/100gm B.W and 2ml/100 gm B.W of virgin olive oil (VOO), respectively. Blood samples were collected weekly from all rats. Whole blood was obtained for determination of some haematological parameters, while sera were collected for the assay of T3 and T4 hormone.
{"title":"EFFECT OF VIRGIN OLIVE OIL SUPPLEMENTATION ON SOME HEMATOLOGIC AND THYROID HORMONES, LEVELS IN RATS","authors":"A. Farahat, A. SawiressF., A. AghwiderA.","doi":"10.21608/JVMR.2019.43332","DOIUrl":"https://doi.org/10.21608/JVMR.2019.43332","url":null,"abstract":"The aim of the present study is to investigate the effect of virgin olive oil on some blood parameters in male Albino rats. Thirty male Sprague Dawley rats, (90-110 g), were used in the present study and were divided into three groups (10 in each), 1st group (control), received basal diet and supplemented with 1ml saline. 2nd and 3rd groups received basal diet, and supplemented daily with 1ml/100gm B.W and 2ml/100 gm B.W of virgin olive oil (VOO), respectively. Blood samples were collected weekly from all rats. Whole blood was obtained for determination of some haematological parameters, while sera were collected for the assay of T3 and T4 hormone.","PeriodicalId":53046,"journal":{"name":"Journal of Veterinary Medical Research","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77896775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-06-01DOI: 10.21608/JVMR.2019.43339
E. F.R., A. A.H., H. H.M., Nabih A.M., Khalifa E., S. S.E.
Bovine respiratory disease (BRD) is the most common and costly diseaseaffecting beef cattle calves in the world. The objectives of this study were to put ahigh light on epidemiology of P. multocida and M. haemolytica as important causesof bovine respiratory disease (BRD) in Egypt Governorates; Giza,El-Fayoum,BeniSuef, Assiut and Sohag. A total number of 406 deep nasal swabs and blood sampleswere collected from 406 bovine calves suffered from respiratory manifestationsreared in different Governorates in Egypt. Bacteriological examination wasachieved and isolation of P. multocida and M. haemolytica were attained. Theoverall prevalence of bothP. multocida and M. haemolytica was of 26.6%; 18.2%for P. multocida and 8.4%for M. haemolytica. EL-Fayoum Governorate showed thehighest prevalences while Beni-Suef Governorate showed the lowest prevalences.P. multocida was singly isolated from 4.9% of cases. While it was mixed with S.aureus, E. coli, Streptococcus spp., both S. aureus and E. coli, both S. aureus andStreptococcus spp. and both E. coli and Streptococcus spp. with percentages of4%, 1.2%, 2.2%, 1.7%, 3.2% and 1.0%, respectively. Meanwhile, M. haemolyticawas isolated as a single isolate from 1.7% of cases while it was mixed with S.aureus, Streptococcus spp., both S. aureus and Streptococcus spp. and both E. coliand Streptococcus spp. with percentages of 2.7%, 1.2%, 2.5% and 0.2%,respectively. In conclusion respiratory manifestations are very important disordersin bovine calves. P. multocida and M.haemolytica are the most common bacteriacausing infection.
{"title":"Bacteriological studies on calves affected with respiratory manifestations","authors":"E. F.R., A. A.H., H. H.M., Nabih A.M., Khalifa E., S. S.E.","doi":"10.21608/JVMR.2019.43339","DOIUrl":"https://doi.org/10.21608/JVMR.2019.43339","url":null,"abstract":"Bovine respiratory disease (BRD) is the most common and costly diseaseaffecting beef cattle calves in the world. The objectives of this study were to put ahigh light on epidemiology of P. multocida and M. haemolytica as important causesof bovine respiratory disease (BRD) in Egypt Governorates; Giza,El-Fayoum,BeniSuef, Assiut and Sohag. A total number of 406 deep nasal swabs and blood sampleswere collected from 406 bovine calves suffered from respiratory manifestationsreared in different Governorates in Egypt. Bacteriological examination wasachieved and isolation of P. multocida and M. haemolytica were attained. Theoverall prevalence of bothP. multocida and M. haemolytica was of 26.6%; 18.2%for P. multocida and 8.4%for M. haemolytica. EL-Fayoum Governorate showed thehighest prevalences while Beni-Suef Governorate showed the lowest prevalences.P. multocida was singly isolated from 4.9% of cases. While it was mixed with S.aureus, E. coli, Streptococcus spp., both S. aureus and E. coli, both S. aureus andStreptococcus spp. and both E. coli and Streptococcus spp. with percentages of4%, 1.2%, 2.2%, 1.7%, 3.2% and 1.0%, respectively. Meanwhile, M. haemolyticawas isolated as a single isolate from 1.7% of cases while it was mixed with S.aureus, Streptococcus spp., both S. aureus and Streptococcus spp. and both E. coliand Streptococcus spp. with percentages of 2.7%, 1.2%, 2.5% and 0.2%,respectively. In conclusion respiratory manifestations are very important disordersin bovine calves. P. multocida and M.haemolytica are the most common bacteriacausing infection.","PeriodicalId":53046,"journal":{"name":"Journal of Veterinary Medical Research","volume":"63 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87971145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-06-01DOI: 10.21608/JVMR.2019.43330
R. G. A., Fathy M. Z.
"> Magnetic resonance imaging (MRI) is a good useful imagingtool for diagnosing foot lameness in equine. Seventeen donkeys of age (6–12 years), gender admitted to the clinic unit of our collage with owners complained that the animals were suffered from incurable lameness in their forelimbs. All forelimb foots were collected at the level of fetlock joint after euthanasia for performing MR imaging .The results showed that, both normal anatomy to the foot using T1-weighted image sequence and detection of different lesions involving many parts of the foot such as collateral ligaments of the DIP joint, both DDFT core and border, both navicular bone medullary cavity and cortex, articular cartilage of DIP joint, distal sesamoidean impair ligament (DSIL) and suspensory ligament of the navicular bone that could not be diagnosed by other diagnostic tools such as X- ray, ultrasound and Computed Tomography. In conclusion, Using MRI in examination of donkey foot lameness is considered as a definitive and accurate diagnostic tool for design suitable treatment regimens for all affections.
{"title":"Magnetic resonance imaging for detection of some foot lesions causing lameness in donkeys (Equus asinus)","authors":"R. G. A., Fathy M. Z.","doi":"10.21608/JVMR.2019.43330","DOIUrl":"https://doi.org/10.21608/JVMR.2019.43330","url":null,"abstract":"\"> Magnetic resonance imaging (MRI) is a good useful imagingtool for diagnosing foot lameness in equine. Seventeen donkeys of age (6–12 years), gender admitted to the clinic unit of our collage with owners complained that the animals were suffered from incurable lameness in their forelimbs. All forelimb foots were collected at the level of fetlock joint after euthanasia for performing MR imaging .The results showed that, both normal anatomy to the foot using T1-weighted image sequence and detection of different lesions involving many parts of the foot such as collateral ligaments of the DIP joint, both DDFT core and border, both navicular bone medullary cavity and cortex, articular cartilage of DIP joint, distal sesamoidean impair ligament (DSIL) and suspensory ligament of the navicular bone that could not be diagnosed by other diagnostic tools such as X- ray, ultrasound and Computed Tomography. In conclusion, Using MRI in examination of donkey foot lameness is considered as a definitive and accurate diagnostic tool for design suitable treatment regimens for all affections.","PeriodicalId":53046,"journal":{"name":"Journal of Veterinary Medical Research","volume":"83 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80903306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-06-01DOI: 10.21608/JVMR.2019.43338
U. Hagag, Mahmoud M.M.
0px; "> Butorphanol is a synthetic opioid commonly used for epidural anesthesia in humaneither alone or in combination with bupivacaine but not in goat. The objective of thepresent study was to evaluate the analgesic effect of epidural butorphanol eitheralone or in combination with bupivacaine. Fifteen adult apparently healthy goatswere randomized into three equal groups to receive a lumbosacral epidural injectionof bupivacaine 0.5 % (0.5 mg/kg), butorphanol 1% (0.08 mg/kg) or theircombination (bupivacaine 0.25 mg/kg and butorphanol 0.04 mg/kg). Animals wereobserved for incoordination of hind limbs, perineal pin prick and sedation at 10minutes interval. Heart rate, respiratory rate and rectal temperature were assessedevery 15 minutes. Epidural butorphanol resulted into significantly (P < 0.05) rapidanalgesia (9.8 ± 1.1 Min) than the butorphanol– bupivacaine combination (12.8 ±0.84 Min) and bupivacaine (16.6 ± 0.55). Butorphanol-bupivacaine combinationprovoked significant ((P < 0.05) prolonged intense analgesia (208 ± 8.36 Min)compared to either bupivacaine (112 ± 8.37) or butorphanol (166 ± 5.48 Min).Ataxia accompanied administration of bupivacaine either alone or in combinationwith butorphanol, while sedation was observed in animals received butorphanolalone or combined with bupivacaine. No significant changes were observed in heartrate, respiratory rate or rectal temperature. The epidural administration ofbutorphanol-bupivacaine combination promoted longer-lasting analgesia in goatswithout motor disturbances compared to bupivacaine alone. This combination mightprove useful clinically to provide analgesia in goats for long-duration perinealobstetrical or surgical procedures.
{"title":"Clinical evaluation of epidural bupivacaine, butorphanol, and butorphanol - bupivacaine combination in goat","authors":"U. Hagag, Mahmoud M.M.","doi":"10.21608/JVMR.2019.43338","DOIUrl":"https://doi.org/10.21608/JVMR.2019.43338","url":null,"abstract":"0px; \"> Butorphanol is a synthetic opioid commonly used for epidural anesthesia in humaneither alone or in combination with bupivacaine but not in goat. The objective of thepresent study was to evaluate the analgesic effect of epidural butorphanol eitheralone or in combination with bupivacaine. Fifteen adult apparently healthy goatswere randomized into three equal groups to receive a lumbosacral epidural injectionof bupivacaine 0.5 % (0.5 mg/kg), butorphanol 1% (0.08 mg/kg) or theircombination (bupivacaine 0.25 mg/kg and butorphanol 0.04 mg/kg). Animals wereobserved for incoordination of hind limbs, perineal pin prick and sedation at 10minutes interval. Heart rate, respiratory rate and rectal temperature were assessedevery 15 minutes. Epidural butorphanol resulted into significantly (P < 0.05) rapidanalgesia (9.8 ± 1.1 Min) than the butorphanol– bupivacaine combination (12.8 ±0.84 Min) and bupivacaine (16.6 ± 0.55). Butorphanol-bupivacaine combinationprovoked significant ((P < 0.05) prolonged intense analgesia (208 ± 8.36 Min)compared to either bupivacaine (112 ± 8.37) or butorphanol (166 ± 5.48 Min).Ataxia accompanied administration of bupivacaine either alone or in combinationwith butorphanol, while sedation was observed in animals received butorphanolalone or combined with bupivacaine. No significant changes were observed in heartrate, respiratory rate or rectal temperature. The epidural administration ofbutorphanol-bupivacaine combination promoted longer-lasting analgesia in goatswithout motor disturbances compared to bupivacaine alone. This combination mightprove useful clinically to provide analgesia in goats for long-duration perinealobstetrical or surgical procedures.","PeriodicalId":53046,"journal":{"name":"Journal of Veterinary Medical Research","volume":"18 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76620601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-06-01DOI: 10.21608/JVMR.2019.43343
Mostafa M. Hammouda, Abdelsattar M. Arafa
In this study a total number of 22 organ samples (including trachea, lung and kidney) from 22 broiler farms from northern Upper Egypt were collected from Mars 2017 to June 2018 from chickens showing clear clinical and pathological signs of Infectious Bronchitis. The samples were prepared and examined by real time RT-PCR for diagnosis of IBV. A total number of 11 samples were positive (50%) which were used for further isolation on SPF eggs by three blind serial passages. Positive samples that showed the pathogenic lesions of IB (curling and dwarfing of embryos) were collected and tested with real time RT-PCR (rRT-PCR) for more confirmation then a part from S1 gene sequence was amplified by RT-PCR and the product was sequenced and the data have been compared with other related IBV strains. The results indicate that the Egyptian virus in this study has an identity percent reached up to 89% with other recent Egyptian isolates. However, it reached 67% with classical vaccine strains like H120 and variant I like CR88 strain. The lowest identity was observed with M41 strain (59%) in this study. The phylogenetic tree compared to other isolates from Middle East and worldwide showed that this isolate is related to the IBV variant 2 group closely related to IBVEg/1265B/2012 strain and the Israeli strain IS/1494/06.
{"title":"Molecular characterization and genotyping of recent isolate of Infectious Bronchitis Virus (IBV) in Egypt","authors":"Mostafa M. Hammouda, Abdelsattar M. Arafa","doi":"10.21608/JVMR.2019.43343","DOIUrl":"https://doi.org/10.21608/JVMR.2019.43343","url":null,"abstract":"In this study a total number of 22 organ samples (including trachea, lung and kidney) from 22 broiler farms from northern Upper Egypt were collected from Mars 2017 to June 2018 from chickens showing clear clinical and pathological signs of Infectious Bronchitis. The samples were prepared and examined by real time RT-PCR for diagnosis of IBV. A total number of 11 samples were positive (50%) which were used for further isolation on SPF eggs by three blind serial passages. Positive samples that showed the pathogenic lesions of IB (curling and dwarfing of embryos) were collected and tested with real time RT-PCR (rRT-PCR) for more confirmation then a part from S1 gene sequence was amplified by RT-PCR and the product was sequenced and the data have been compared with other related IBV strains. The results indicate that the Egyptian virus in this study has an identity percent reached up to 89% with other recent Egyptian isolates. However, it reached 67% with classical vaccine strains like H120 and variant I like CR88 strain. The lowest identity was observed with M41 strain (59%) in this study. The phylogenetic tree compared to other isolates from Middle East and worldwide showed that this isolate is related to the IBV variant 2 group closely related to IBVEg/1265B/2012 strain and the Israeli strain IS/1494/06.","PeriodicalId":53046,"journal":{"name":"Journal of Veterinary Medical Research","volume":"52 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85870427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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