Pub Date : 2022-04-01DOI: 10.21608/jvmr.2022.113186.1046
Adel F El Kholy, Mohamed A. Elshater, Marwa Abd El Gawad, M. Zeinhom
{"title":"Prevalence of Enterotoxigenic S. aureus in Table Eggs in El-Fayoum City, Egypt","authors":"Adel F El Kholy, Mohamed A. Elshater, Marwa Abd El Gawad, M. Zeinhom","doi":"10.21608/jvmr.2022.113186.1046","DOIUrl":"https://doi.org/10.21608/jvmr.2022.113186.1046","url":null,"abstract":"","PeriodicalId":53046,"journal":{"name":"Journal of Veterinary Medical Research","volume":"23 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80111767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-12-01DOI: 10.21608/jvmr.2021.105032.1045
M. Bashandy, Hanan M Saeed, W. Ahmed, M. Ibrahim, O. Shehata
{"title":"Ameliorative Effect of Cerium Oxide Nanoparticles against Cadmium Nephrotoxicity in Male Albino Rats","authors":"M. Bashandy, Hanan M Saeed, W. Ahmed, M. Ibrahim, O. Shehata","doi":"10.21608/jvmr.2021.105032.1045","DOIUrl":"https://doi.org/10.21608/jvmr.2021.105032.1045","url":null,"abstract":"","PeriodicalId":53046,"journal":{"name":"Journal of Veterinary Medical Research","volume":"79 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76680158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-12-01DOI: 10.21608/jvmr.2021.104022.1044
G. Hassan, H. Salem, M. Zeinhom
{"title":"Incidence of Stenotrophomonas Species in Milk and Some Dairy Products in Beni-Suef Province, Egypt","authors":"G. Hassan, H. Salem, M. Zeinhom","doi":"10.21608/jvmr.2021.104022.1044","DOIUrl":"https://doi.org/10.21608/jvmr.2021.104022.1044","url":null,"abstract":"","PeriodicalId":53046,"journal":{"name":"Journal of Veterinary Medical Research","volume":"16 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90848291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-12-01DOI: 10.21608/jvmr.2021.92807.1042
M. El-Sebelgy, H. Madbouly, S. Tamam, N. Ata, K. Zaher
{"title":"Studies on Bluetongue virus (BTV) from Ruminant Collected Samples during 2016 in Egypt","authors":"M. El-Sebelgy, H. Madbouly, S. Tamam, N. Ata, K. Zaher","doi":"10.21608/jvmr.2021.92807.1042","DOIUrl":"https://doi.org/10.21608/jvmr.2021.92807.1042","url":null,"abstract":"","PeriodicalId":53046,"journal":{"name":"Journal of Veterinary Medical Research","volume":"105 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78652148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-11-08DOI: 10.21608/jvmr.2021.103336.1043
Mostafa Tamam
{"title":"Bacteriological Quality of Raw Milk in Beni-Suef Governorate, Egypt","authors":"Mostafa Tamam","doi":"10.21608/jvmr.2021.103336.1043","DOIUrl":"https://doi.org/10.21608/jvmr.2021.103336.1043","url":null,"abstract":"","PeriodicalId":53046,"journal":{"name":"Journal of Veterinary Medical Research","volume":"14 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74473914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-08-28DOI: 10.21608/jvmr.2021.78498.1038
I. Raheel, A. Orabi, Nehal Tag
E. coli is one of the most important pathogenic bacteria in poultry industry. It causes high economic losses, high morbidity and mortality rates due to collibacillosis. Moreover, it is multidrug resistant bacteria. In the last few years, scientists directed their efforts to the use of essential oils which have antimicrobial actions by inhibition of some virulence properties such as biofilm formation and quorum sensing. In this study, out of 150 examined samples, 139 were found to be bacteriologically positive for G-ve bacilli (92.7%) and 84 E. coli isolates (60.4%) were recovered from broilers suffering from pericarditis. Twenty random recovered isolates were selected for antimicrobial susceptibility test. All isolates were completely resistant to amoxicillin, gentamicin, lincomycin and florfenicol and 90% resistant to trimethoprim- sulfamethoxazole, kanamycin, ciprofloxacin and doxycycline while sensitivity to amoxicillin+clavulanic acid and cefotaxime was 40% and 20% respectively. We screened 15 randomly selected MDR isolates by CR assay for detection of biofilm formation at which CR positive isolates represent 80%. Cinnamon and clove oils showed antimicrobial effect and cause down regulation in both biofilm gene (sfa) and quorum sensing gene (luxS).
{"title":"Down Regulation of Biofilm and Quorum Sensing Genes in E. coli Isolated from Broiler Chickens Pericarditis Lesions by the Action of Some Essential Oils","authors":"I. Raheel, A. Orabi, Nehal Tag","doi":"10.21608/jvmr.2021.78498.1038","DOIUrl":"https://doi.org/10.21608/jvmr.2021.78498.1038","url":null,"abstract":"E. coli is one of the most important pathogenic bacteria in poultry industry. It causes high economic losses, high morbidity and mortality rates due to collibacillosis. Moreover, it is multidrug resistant bacteria. In the last few years, scientists directed their efforts to the use of essential oils which have antimicrobial actions by inhibition of some virulence properties such as biofilm formation and quorum sensing. In this study, out of 150 examined samples, 139 were found to be bacteriologically positive for G-ve bacilli (92.7%) and 84 E. coli isolates (60.4%) were recovered from broilers suffering from pericarditis. Twenty random recovered isolates were selected for antimicrobial susceptibility test. All isolates were completely resistant to amoxicillin, gentamicin, lincomycin and florfenicol and 90% resistant to trimethoprim- sulfamethoxazole, kanamycin, ciprofloxacin and doxycycline while sensitivity to amoxicillin+clavulanic acid and cefotaxime was 40% and 20% respectively. We screened 15 randomly selected MDR isolates by CR assay for detection of biofilm formation at which CR positive isolates represent 80%. Cinnamon and clove oils showed antimicrobial effect and cause down regulation in both biofilm gene (sfa) and quorum sensing gene (luxS).","PeriodicalId":53046,"journal":{"name":"Journal of Veterinary Medical Research","volume":"26 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86450084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-08-28DOI: 10.21608/jvmr.2021.89244.1041
W. Hassan, H. Hassan, W. Hassan, Salama A. S. Shany, G. Osman
Salmonellosis is one of the most important problems in poultry industry and a critical food safety hazard. In the present study the prevalence of avian Salmonellosis was studied in different farms of broiler chickens in Beni Suef Governorate, Egypt during the period from January to April 2020. A total of 140 samples were taken from slaughtered diseased or freshly dead broiler chickens aged from one to 35 days. Bacteriological examination revealed that 7.14% of the samples were Salmonella positive. Serotyping of Salmonella isolates showed that S. kentucky, S. blegdam and S. virchow were recognized at rates of 40%, 30% and 30%, respectively. Antimicrobial susceptibility test revealed that all salmonella isolates were multidrug resistant (MDR). All isolates were resistant to oxytetracycline (100%) while 90% were resistant to amoxicillin-clavulanic acid, cefotaxime, sulfamethoxazole- trimethoprim and norofloxacin. On the other hand, 80% of isolates were sensitive to fosfomycin and nitrofurantoin. Results of screening of some MDR isolates by multiplex PCR for detection of some virulence genes showed that all the tested isolates (100%) had invA, stn, spvC genes meanwhile pefA was not detected in any isolate.
{"title":"Identification and Characterization of Salmonella Species Isolated from Broiler Chickens","authors":"W. Hassan, H. Hassan, W. Hassan, Salama A. S. Shany, G. Osman","doi":"10.21608/jvmr.2021.89244.1041","DOIUrl":"https://doi.org/10.21608/jvmr.2021.89244.1041","url":null,"abstract":"Salmonellosis is one of the most important problems in poultry industry and a critical food safety hazard. In the present study the prevalence of avian Salmonellosis was studied in different farms of broiler chickens in Beni Suef Governorate, Egypt during the period from January to April 2020. A total of 140 samples were taken from slaughtered diseased or freshly dead broiler chickens aged from one to 35 days. Bacteriological examination revealed that 7.14% of the samples were Salmonella positive. Serotyping of Salmonella isolates showed that S. kentucky, S. blegdam and S. virchow were recognized at rates of 40%, 30% and 30%, respectively. Antimicrobial susceptibility test revealed that all salmonella isolates were multidrug resistant (MDR). All isolates were resistant to oxytetracycline (100%) while 90% were resistant to amoxicillin-clavulanic acid, cefotaxime, sulfamethoxazole- trimethoprim and norofloxacin. On the other hand, 80% of isolates were sensitive to fosfomycin and nitrofurantoin. Results of screening of some MDR isolates by multiplex PCR for detection of some virulence genes showed that all the tested isolates (100%) had invA, stn, spvC genes meanwhile pefA was not detected in any isolate.","PeriodicalId":53046,"journal":{"name":"Journal of Veterinary Medical Research","volume":"2 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73072738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-08-28DOI: 10.21608/jvmr.2021.88953.1040
H. I. Hosein, M. Hamdy, A. Zaitoun, A. Menshawy, Sherin Rouby, B. Madkour, Amira Mazeed, Aml M. Abdel-Raouf
Brucellosis is a major constraint to livestock production that still enzootic in livestock in many developing countries including Egypt. This study was conducted with the general objective of establishing the bacteriological status of bovine brucellosis in 15 governorates in Egypt during 2020-2021 to determine the circulating Brucella species on bacteriological and molecular basis. Clinical samples collected included milk or udder secretions, vaginal discharges, fetal membranes and stomach contents of aborted fetuses from dairy cows with history of brucellosis. In addition, lymph nodes (retropharyngeal, prescapular, prefemoral, internal iliac and supramammary) from carcasses of serologically positive animals were obtained from different localities for isolation and identification of Brucella organisms. A total of 136 Brucella isolates were recovered from cattle in different governorates, Egypt. These include, 107 isolates of Brucella melitensis biovar 3 identified on bacteriological and molecular basis from Aswan, Beheira, Beni Suef, Dakahlia, Damietta, Fayoum, Gharbia, Giza, Ismailia, Kafr El-Sheikh, Luxor, Monufia, Port Said, Qalyubia and Sharqia governorates. On the other hand, 29 Brucella abortus biovar 1 isolates were recovered from cattle from Beni Suef, Dakahlia, Damietta, Kafr El-Sheikh, Monufia, Port Said and Sharqia governorates. Molecular identification using primer sequences targeting IS711 gene confirmed Brucella on genus level. Multiplex PCR has amplified four fragments of 450bp, 587 bp, 1071 bp, and1682 bp characteristic for B. melitensis biovar 3, and three fragments of 450bp, 587 bp, and 1682 bp for B. abortus biovar 1. The identification of Brucella spp. in different farm animals of 15 Egyptian governorates highlights the dynamics and role of cattle in dissemination of Brucella infection all over the country. The obtained results indicate that the actual Brucellosis status during the years 2020 and 2021 refers to that B. melitensis biovar 3 and B. abortus biovar 1 are the prevalent types circulating in different Egyptian governorates.
{"title":"Brucella Prevalent Strains Circulating in Egypt during 2020-2021: Bacteriological and Molecular Study","authors":"H. I. Hosein, M. Hamdy, A. Zaitoun, A. Menshawy, Sherin Rouby, B. Madkour, Amira Mazeed, Aml M. Abdel-Raouf","doi":"10.21608/jvmr.2021.88953.1040","DOIUrl":"https://doi.org/10.21608/jvmr.2021.88953.1040","url":null,"abstract":"Brucellosis is a major constraint to livestock production that still enzootic in livestock in many developing countries including Egypt. This study was conducted with the general objective of establishing the bacteriological status of bovine brucellosis in 15 governorates in Egypt during 2020-2021 to determine the circulating Brucella species on bacteriological and molecular basis. Clinical samples collected included milk or udder secretions, vaginal discharges, fetal membranes and stomach contents of aborted fetuses from dairy cows with history of brucellosis. In addition, lymph nodes (retropharyngeal, prescapular, prefemoral, internal iliac and supramammary) from carcasses of serologically positive animals were obtained from different localities for isolation and identification of Brucella organisms. A total of 136 Brucella isolates were recovered from cattle in different governorates, Egypt. These include, 107 isolates of Brucella melitensis biovar 3 identified on bacteriological and molecular basis from Aswan, Beheira, Beni Suef, Dakahlia, Damietta, Fayoum, Gharbia, Giza, Ismailia, Kafr El-Sheikh, Luxor, Monufia, Port Said, Qalyubia and Sharqia governorates. On the other hand, 29 Brucella abortus biovar 1 isolates were recovered from cattle from Beni Suef, Dakahlia, Damietta, Kafr El-Sheikh, Monufia, Port Said and Sharqia governorates. Molecular identification using primer sequences targeting IS711 gene confirmed Brucella on genus level. Multiplex PCR has amplified four fragments of 450bp, 587 bp, 1071 bp, and1682 bp characteristic for B. melitensis biovar 3, and three fragments of 450bp, 587 bp, and 1682 bp for B. abortus biovar 1. The identification of Brucella spp. in different farm animals of 15 Egyptian governorates highlights the dynamics and role of cattle in dissemination of Brucella infection all over the country. The obtained results indicate that the actual Brucellosis status during the years 2020 and 2021 refers to that B. melitensis biovar 3 and B. abortus biovar 1 are the prevalent types circulating in different Egyptian governorates.","PeriodicalId":53046,"journal":{"name":"Journal of Veterinary Medical Research","volume":"118 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81263260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-08-28DOI: 10.21608/jvmr.2021.84001.1039
Marta GARCIA PIQUERES, P. FORÉS JACKSON
{"title":"Evaluation of Kinesio Taping Applied to the Equine Thoracolumbar Spine: Clinical Response and Mechanical Nociceptive Threshold","authors":"Marta GARCIA PIQUERES, P. FORÉS JACKSON","doi":"10.21608/jvmr.2021.84001.1039","DOIUrl":"https://doi.org/10.21608/jvmr.2021.84001.1039","url":null,"abstract":"","PeriodicalId":53046,"journal":{"name":"Journal of Veterinary Medical Research","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80623571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-08-28DOI: 10.21608/jvmr.2021.73921.1037
Lutfi Bakar, A. Hussein, S. Tamam, H. Madbouly
Massive outbreaks of suspected LSDV in cattle population were observed during 2017 and 2018 at Beni-Suef governorate. Samples from diseased cattle, previously SPPV vaccinated with SPPV, were inoculated in embryonated chicken eggs (ECE) using chorio-allantoic membrane (CAM) route. Histopathological examination of the inoculated CAMs showed large eosinophilic intracytoplasmic inclusion bodies characteristic for LSDV. The inoculated CAMs were hemorrhagic with congestion blood vessels appeared by the 1st passage then become more pronounced after the second - fourth passages. Characteristic pock lesions were observed after the 1st passage and become clear after the third passage and become clearly observed 4 days post inoculation. For molecular identification, DNA was extracted from a pool of the infected CAMs. Two pairs of primers specific for LSDV including one flanking a 554 bp product of the G gene and second flanking 172 of the RP030 gene were used. In conclusion, LSDV infections have been detected and the virus has been isolated and identified by PCR from cattle previously vaccinated with SPPV vaccine during the summer of 2018.
{"title":"Isolation of Lumpy Skin Disease Virus Isolated from SPPV Vaccinated Cattle","authors":"Lutfi Bakar, A. Hussein, S. Tamam, H. Madbouly","doi":"10.21608/jvmr.2021.73921.1037","DOIUrl":"https://doi.org/10.21608/jvmr.2021.73921.1037","url":null,"abstract":"Massive outbreaks of suspected LSDV in cattle population were observed during 2017 and 2018 at Beni-Suef governorate. Samples from diseased cattle, previously SPPV vaccinated with SPPV, were inoculated in embryonated chicken eggs (ECE) using chorio-allantoic membrane (CAM) route. Histopathological examination of the inoculated CAMs showed large eosinophilic intracytoplasmic inclusion bodies characteristic for LSDV. The inoculated CAMs were hemorrhagic with congestion blood vessels appeared by the 1st passage then become more pronounced after the second - fourth passages. Characteristic pock lesions were observed after the 1st passage and become clear after the third passage and become clearly observed 4 days post inoculation. For molecular identification, DNA was extracted from a pool of the infected CAMs. Two pairs of primers specific for LSDV including one flanking a 554 bp product of the G gene and second flanking 172 of the RP030 gene were used. In conclusion, LSDV infections have been detected and the virus has been isolated and identified by PCR from cattle previously vaccinated with SPPV vaccine during the summer of 2018.","PeriodicalId":53046,"journal":{"name":"Journal of Veterinary Medical Research","volume":"51 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90639115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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