{"title":"Human mesenchymal stromal cells alginate encapsulation beneficial effect for storage at ambient temperature under hypoxic conditions","authors":"Oleksandra Hubenia, Yuriy Kot, N. Trufanova","doi":"10.15407/cryo32.04.296a","DOIUrl":"https://doi.org/10.15407/cryo32.04.296a","url":null,"abstract":"","PeriodicalId":53457,"journal":{"name":"Problems of Cryobiology and Cryomedicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46264837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Y. Smolyaninova, V. Shigimaga, Lyudmila Popivnenko, I. Kovalenko
In this work, we have studied the impact of various stages of cryopreservation by vitrification in ethylene glycolsucrose medium on plasma membranes adhesive properties of 8-cell murine embryos and their specific electrical conductivity. Embryos were divided into the following experimental groups: the control, group 1 (incubation in vitrification medium) and group 2 (complete cycle of cryopreservation). The embryo exposure to vitrification medium did not affect their ability to cell-to-cell adhesion. After a complete cycle of cryopreservation, no embryo adhesion was observed. Electrical conductivity of embryo membranes was determined using the pulsed conductometry. After incubation in vitrification medium, their resistance to electric pulse was shown to decrease, that was manifested in the phenomenon of irreversible electric breakdown. The average values of electrical conductivity varied within the following ranges: ((12.1 ± 1.5)...(55.5 ± 2.6)), ((28.7 ± 5.7)...(44.± 8.9)), ((31.0 ± 9.3)...(87.9 ± 26.1)) μS/cm in the control, groups 1 and 2, respectively. These findings may be explained by appearance of first structural disorders in lipid bilayer of embryo membranes even at the stage of their incubation in vitrification medium. The lack of adhesive properties of blastomeres after vitrification-warming testified to a damage to the membrane adhesion proteins.
{"title":"Cell-to-Cell Adhesion and Electrical Parameters of Murine Embryo Membranes After Cryopreservation by Vitrification","authors":"Y. Smolyaninova, V. Shigimaga, Lyudmila Popivnenko, I. Kovalenko","doi":"10.15407/cryo32.04.256","DOIUrl":"https://doi.org/10.15407/cryo32.04.256","url":null,"abstract":"In this work, we have studied the impact of various stages of cryopreservation by vitrification in ethylene glycolsucrose medium on plasma membranes adhesive properties of 8-cell murine embryos and their specific electrical conductivity. Embryos were divided into the following experimental groups: the control, group 1 (incubation in vitrification medium) and group 2 (complete cycle of cryopreservation). The embryo exposure to vitrification medium did not affect their ability to cell-to-cell adhesion. After a complete cycle of cryopreservation, no embryo adhesion was observed. Electrical conductivity of embryo membranes was determined using the pulsed conductometry. After incubation in vitrification medium, their resistance to electric pulse was shown to decrease, that was manifested in the phenomenon of irreversible electric breakdown. The average values of electrical conductivity varied within the following ranges: ((12.1 ± 1.5)...(55.5 ± 2.6)), ((28.7 ± 5.7)...(44.± 8.9)), ((31.0 ± 9.3)...(87.9 ± 26.1)) μS/cm in the control, groups 1 and 2, respectively. These findings may be explained by appearance of first structural disorders in lipid bilayer of embryo membranes even at the stage of their incubation in vitrification medium. The lack of adhesive properties of blastomeres after vitrification-warming testified to a damage to the membrane adhesion proteins.","PeriodicalId":53457,"journal":{"name":"Problems of Cryobiology and Cryomedicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43140651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Characteristic of migration in culture of newborn and fetal rat neural cells","authors":"S. Vsevolodska, O. Sukach, O. Ochenashko","doi":"10.15407/cryo32.04.306","DOIUrl":"https://doi.org/10.15407/cryo32.04.306","url":null,"abstract":"","PeriodicalId":53457,"journal":{"name":"Problems of Cryobiology and Cryomedicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45270336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Miroslava Jandová, P. Měřička, J. Gregor, M. Lánská, D. Vokurková, I. Fátorová, A. Bezrouk, J. Radocha
{"title":"Influence of the Me2SO removal on key parameters of cryopreserved autologous peripheral progenitor cells concentrates: a retrospective study in patients with primary or secondary amyloidosis","authors":"Miroslava Jandová, P. Měřička, J. Gregor, M. Lánská, D. Vokurková, I. Fátorová, A. Bezrouk, J. Radocha","doi":"10.15407/cryo32.04.298a","DOIUrl":"https://doi.org/10.15407/cryo32.04.298a","url":null,"abstract":"","PeriodicalId":53457,"journal":{"name":"Problems of Cryobiology and Cryomedicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46912450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Alexandra Popovičová, K. Fabianová, M. Martončíková, A. Raček, E. Račeková
{"title":"Neurons of the olfactory neurogenic region of the rat: a morphological study","authors":"Alexandra Popovičová, K. Fabianová, M. Martončíková, A. Raček, E. Račeková","doi":"10.15407/cryo32.04.309","DOIUrl":"https://doi.org/10.15407/cryo32.04.309","url":null,"abstract":"","PeriodicalId":53457,"journal":{"name":"Problems of Cryobiology and Cryomedicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45428797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In this report, we have studied a cardioprotective effect of low molecular weight (below 5 kDa) fraction derived from cattle cord blood in a rat model of infarction-like cardiovasopathy. The myocardial infarction development was initiated in the animals, maintained for two months on a diet with excessive intake of fat, calcium and sodium salts, by making hypoxia-hypercapnia in them. The use of this model allowed inducing the infarction-like cardiovasopathy, comparable by many respects with the clinical picture of myocardial infarction, which was confirmed by electrocardiography, enzyme-linked immunosorbent assay and histological analysis. Preliminary administration of low molecular weight (below 5 kDa) fraction of cattle cord blood, as well as Actovegin did not prevent the heart attack, but increased the endurance of rats to hypoxia-hypercapnia and reduced the damage rate to myocardium. A preventive administration of low molecular weight fraction of cord blood was proven to mitigate the risk of hypoxia-induced damages to myocardium.
{"title":"Cardioprotective Effect of Low Molecular Weight (Below 5 kDa) Fraction of Cattle Cord Blood Cryohemolysate","authors":"I. Shcheniavsky, I. Kovalenko, O. Gulevsky","doi":"10.15407/cryo32.03.206","DOIUrl":"https://doi.org/10.15407/cryo32.03.206","url":null,"abstract":"In this report, we have studied a cardioprotective effect of low molecular weight (below 5 kDa) fraction derived from cattle cord blood in a rat model of infarction-like cardiovasopathy. The myocardial infarction development was initiated in the animals, maintained for two months on a diet with excessive intake of fat, calcium and sodium salts, by making hypoxia-hypercapnia in them. The use of this model allowed inducing the infarction-like cardiovasopathy, comparable by many respects with the clinical picture of myocardial infarction, which was confirmed by electrocardiography, enzyme-linked immunosorbent assay and histological analysis. Preliminary administration of low molecular weight (below 5 kDa) fraction of cattle cord blood, as well as Actovegin did not prevent the heart attack, but increased the endurance of rats to hypoxia-hypercapnia and reduced the damage rate to myocardium. A preventive administration of low molecular weight fraction of cord blood was proven to mitigate the risk of hypoxia-induced damages to myocardium.","PeriodicalId":53457,"journal":{"name":"Problems of Cryobiology and Cryomedicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43671027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Here, we have studied the rehabilitation efficiency for patients with spinal degenerative joint diseases using the wholebody extreme air cryotherapy (WBEACT) according to our own technique. The assessment of pain intensity on visual-analogue scale upon completing the rehabilitation therapy showed the pain disappearance in 68.4% of patients, and its significant reduction in 31.6%; increased daily motor activity, and decreased restrictions during walking in 90.8% of cases; improved well-being in 35.7%; enhanced activity in 43.9%, and mood change in 38.8%. Each WBEACT session increased the muscle strength and endurance for both genders of patients, thus testifying to a stimulating effect of cryotherapy to human body. The WBEACT application for rehabilitation of patients with spinal degenerative joint diseases enabled to normalize the psycho-emotional state as quickly as possible and improve functional state of neuromuscular apparatus, i. e. to reduce the pain and muscle tonic syndromes, strengthen the muscular skeleton, and increase the range of motion and endurance while standing, sitting and walking.
{"title":"Medical and Psychological Rehabilitation for Patients with Spinal Degenerative Joint Diseases Using the Whole-Body Extreme Air Cryotherapy","authors":"O. Panchenko, A. Kabantseva","doi":"10.15407/cryo32.03.226","DOIUrl":"https://doi.org/10.15407/cryo32.03.226","url":null,"abstract":"Here, we have studied the rehabilitation efficiency for patients with spinal degenerative joint diseases using the wholebody extreme air cryotherapy (WBEACT) according to our own technique. The assessment of pain intensity on visual-analogue scale upon completing the rehabilitation therapy showed the pain disappearance in 68.4% of patients, and its significant reduction in 31.6%; increased daily motor activity, and decreased restrictions during walking in 90.8% of cases; improved well-being in 35.7%; enhanced activity in 43.9%, and mood change in 38.8%. Each WBEACT session increased the muscle strength and endurance for both genders of patients, thus testifying to a stimulating effect of cryotherapy to human body. The WBEACT application for rehabilitation of patients with spinal degenerative joint diseases enabled to normalize the psycho-emotional state as quickly as possible and improve functional state of neuromuscular apparatus, i. e. to reduce the pain and muscle tonic syndromes, strengthen the muscular skeleton, and increase the range of motion and endurance while standing, sitting and walking.","PeriodicalId":53457,"journal":{"name":"Problems of Cryobiology and Cryomedicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48828953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nadiia Shevchenko, G. Kovalenko, N. Bashtan, A. Mozgovska, Tatiana Miroshnichenko, T. Strybul, T. Ivchenko, O. Kuts
In this work, we have studied the impact of ozonation, freezing down to liquid nitrogen temperature and their combination on sowing quality and crop yield for Dii cultivar beetroot seeds. The optimal moisture, ozone dose within the ozone-air mixture, and the exposure time, not reducing the germination rate, were determined. The ozonation, freezing, and their combined effect affected neither the seed germination in laboratory, nor biometric indices in a field. The seed freezing down to liquid nitrogen temperature was found to significantly increase its crop yield. Biochemical analysis of root crops showed all the ways of presowing treatment to reduce the content of total sugars and betanine. After freezing and ozonation, the amount of dry matter decreased, but after combined exposure it augmented. Ozonation and subsequent freezing lowered the content of ascorbic acid. Significantly reduced studied parameters did not reach the minimum values specific to this crop. Freezing and a combined impact of the studied factors on the seeds increased the nitrate content in root crops, which did not exceed the maximum allowable rate.
{"title":"Physical Factors of Beetroot Seed Presowing Treatment Affect Sowing Quality and Crop Yield","authors":"Nadiia Shevchenko, G. Kovalenko, N. Bashtan, A. Mozgovska, Tatiana Miroshnichenko, T. Strybul, T. Ivchenko, O. Kuts","doi":"10.15407/cryo32.03.183","DOIUrl":"https://doi.org/10.15407/cryo32.03.183","url":null,"abstract":"In this work, we have studied the impact of ozonation, freezing down to liquid nitrogen temperature and their combination on sowing quality and crop yield for Dii cultivar beetroot seeds. The optimal moisture, ozone dose within the ozone-air mixture, and the exposure time, not reducing the germination rate, were determined. The ozonation, freezing, and their combined effect affected neither the seed germination in laboratory, nor biometric indices in a field. The seed freezing down to liquid nitrogen temperature was found to significantly increase its crop yield. Biochemical analysis of root crops showed all the ways of presowing treatment to reduce the content of total sugars and betanine. After freezing and ozonation, the amount of dry matter decreased, but after combined exposure it augmented. Ozonation and subsequent freezing lowered the content of ascorbic acid. Significantly reduced studied parameters did not reach the minimum values specific to this crop. Freezing and a combined impact of the studied factors on the seeds increased the nitrate content in root crops, which did not exceed the maximum allowable rate.","PeriodicalId":53457,"journal":{"name":"Problems of Cryobiology and Cryomedicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45153331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Here, we have presented the data describing the cryopreservation impact on DNA status of domestic and farm animal sperm, and outlined the main causes of DNA fragmentation and methods of its detection and DNA integrity preservation. The current methods to identify the DNA fragmentation rate have been shown to be quite efficient, but the choice for the optimal way is stipulated by animal species. The oxidative stress caused by an increased content of reactive oxygen species was recognized as the main mechanism in DNA cryodamage. To prevent this negative effect on germ genetic material, many protective media and rehabilitation solutions supplemented with antioxidants (non-enzymatic, enzymatic compounds and nanoparticles) have been developed. Thus, determining the sperm DNA fragmentation rate and maintaining its integrity are the necessary steps to improve the efficiency of domestic animal gamete cryopreservation as a part of reproductive technologies.
{"title":"Sperm DNA Cryodamage in Domestic and Farm Animals: Detection Methods and Ways of DNA Integrity Improvement","authors":"T. Yurchuk, O. Pavlovych, M. Petrushko","doi":"10.15407/cryo32.03.171","DOIUrl":"https://doi.org/10.15407/cryo32.03.171","url":null,"abstract":"Here, we have presented the data describing the cryopreservation impact on DNA status of domestic and farm animal sperm, and outlined the main causes of DNA fragmentation and methods of its detection and DNA integrity preservation. The current methods to identify the DNA fragmentation rate have been shown to be quite efficient, but the choice for the optimal way is stipulated by animal species. The oxidative stress caused by an increased content of reactive oxygen species was recognized as the main mechanism in DNA cryodamage. To prevent this negative effect on germ genetic material, many protective media and rehabilitation solutions supplemented with antioxidants (non-enzymatic, enzymatic compounds and nanoparticles) have been developed. Thus, determining the sperm DNA fragmentation rate and maintaining its integrity are the necessary steps to improve the efficiency of domestic animal gamete cryopreservation as a part of reproductive technologies.","PeriodicalId":53457,"journal":{"name":"Problems of Cryobiology and Cryomedicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42640263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The paper presents the findings on the impact of cold exposure at 4°C on chlorophylogenesis, hemogenesis and rhizogenesis in callus culture of winter wheat cultivars. The patterns of morphogenesis in the soft winter wheat in vitro culture during exposure to positive low temperature of various durations (15, 30 and 45 days) have been established. The callus culture of 2–3 passages of cv. Doridna, Statna and Astet was used. The results of experiments showed the preliminary vernalization of calluses for 30 and 45 days to strongly stimulate the chlorophylogenesis and hemogenesis, thereby increasing the frequency of obtaining the regenerant plants during further cultivation. The efficiency of these processes depended on the original cultivar genotype and cold treatment duration. The vernalization, associated with the VRN genes expression, manifested in morphogenesis change, was assumed to occur in the soft winter wheat in vitro culture.
{"title":"Morphogenetic Responses of Triticum aestivum L. Calus Culture During Long-Term Effect of Low Positive Temperatures","authors":"O. Avksentieva, V. Zhmurko, Viktoriia Chumakova","doi":"10.15407/cryo32.03.214","DOIUrl":"https://doi.org/10.15407/cryo32.03.214","url":null,"abstract":"The paper presents the findings on the impact of cold exposure at 4°C on chlorophylogenesis, hemogenesis and rhizogenesis in callus culture of winter wheat cultivars. The patterns of morphogenesis in the soft winter wheat in vitro culture during exposure to positive low temperature of various durations (15, 30 and 45 days) have been established. The callus culture of 2–3 passages of cv. Doridna, Statna and Astet was used. The results of experiments showed the preliminary vernalization of calluses for 30 and 45 days to strongly stimulate the chlorophylogenesis and hemogenesis, thereby increasing the frequency of obtaining the regenerant plants during further cultivation. The efficiency of these processes depended on the original cultivar genotype and cold treatment duration. The vernalization, associated with the VRN genes expression, manifested in morphogenesis change, was assumed to occur in the soft winter wheat in vitro culture.","PeriodicalId":53457,"journal":{"name":"Problems of Cryobiology and Cryomedicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44360610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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