River plume-impacted shelf marginal seas exhibit strong carbon sequestration potential due to their high biological productivity. However, frequent coastal upwelling events complicate the carbon source-sink dynamics because of the competing effects on seawater partial pressure of carbon dioxide (pCO2): the upwelling of dissolved inorganic carbon (DIC)-rich deep waters initially elevate surface pCO2, while subsequent biological uptake lowers it. As a case study, we used a novel wave-driven profiler to obtain high-resolution vertical profiles in the Changjiang plume-impacted shelf area (CPS) and to investigate upwelling-induced variability in pCO2 and carbon source-sink dynamics. The observations were conducted near Gouqi Island, where coastal upwelling frequently occurs. Based on a pCO2 mass balance model, we found that biological processes (contributing 30.6% to pCO2 increase) and physical transport (contributing 21.2% to pCO2 decrease) jointly dominated hourly mixed layer pCO2 variability in the study area. Importantly, we found that αSBW (shelf bottom water fraction) served as a good quantitative proxy for upwelling intensity, with each 1% increase in αSBW associated with a 6-μatm increase in mixed layer. Given the significantly higher mean αSBW values during 20–22 August (34 ± 5%) than 28–30 August (11 ± 7%), we defined the former as the upwelling period and the latter as the post-upwelling period. The air-sea CO2 flux (