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[Proteome Analysis for Identification of the Origin of Biological Foreign Substances]. 生物外来物质来源鉴定的蛋白质组学分析
IF 0.3 4区 农林科学 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2022-01-01 DOI: 10.3358/shokueishi.63.218
Yukako Sato, Tomoko Dewa, Koji Suzuki, Yasushi Nagatomi, Tetsuro Magarifuchi, Yasuhisa Miyamoto

We have developed a method for determination of the species of origin of biological foreign substances using proteomics analysis technology. That is, the amino acid sequence of the tryptic digested product of the protein extracted from the foreign substance is determined by high-resolution LC-MS, and the amino acid sequence is collated with a public protein database to determine the origin species of the foreign substance. As a result of testing meat (beef, pork, chicken) and egg (chicken, quail) from known origin as simulated foreign substances, we were able to find species-specific amino acid sequences for each species, suggesting that the developed method is useful for species discrimination of a foreign substances that have been heat-treated with retort, this method is potentially useful to complement DNA analysis, for determination of the species of origin of foreign substances.

我们开发了一种利用蛋白质组学分析技术确定生物异物来源种类的方法。即通过高分辨率LC-MS测定从异物中提取的蛋白质的色氨酸消化产物的氨基酸序列,并将氨基酸序列与公共蛋白质数据库进行比对,以确定异物的起源物种。将已知来源的肉类(牛肉、猪肉、鸡肉)和鸡蛋(鸡肉、鹌鹑)作为模拟外来物质进行测试,我们能够找到每个物种的物种特异性氨基酸序列,这表明所开发的方法可用于用蒸煮液热处理的外来物质的物种区分,该方法有可能用于补充DNA分析,以确定外来物质的物种来源。
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引用次数: 0
[Determination of Quinclorac in Livestock Products by LC-MS/MS]. [LC-MS/MS法测定畜产品中喹诺灭]。
IF 0.3 4区 农林科学 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2022-01-01 DOI: 10.3358/shokueishi.63.177
Yutaro Takewaki, Ryo Okabe, Satoru Nemoto, Mitsutoshi Aoyagi

A liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based method was developed for determining quinclorac in livestock products. Quinclorac was extracted from the samples using a solution of acetone and hydrochloric acid mixed in a 99 : 1 ratio. The crude extract was purified with ethyl acetate under basic conditions, followed by quinclorac extraction with ethyl acetate under acidic conditions and analysis using LC-MS/MS. The average recoveries of quinclorac from five livestock products (n=5) fortified at the maximum residue limits or 0.01 mg/kg ranged from 85.6 to 93.5%, with the precision of repeatability ranging from 1.7 to 6.8%. The quantification limit in this analytical method was 0.01 mg/kg. These results suggest that the developed method is useful for analyzing quinclorac in livestock products.

建立了一种液相色谱-串联质谱(LC-MS/MS)法测定畜产品中喹诺啉的方法。用丙酮和盐酸以99:1的比例混合的溶液从样品中提取喹洛昔。粗提物在碱性条件下用乙酸乙酯纯化,在酸性条件下用乙酸乙酯提取,用LC-MS/MS分析。5种畜产品(n=5)在最大残留限量为0.01 mg/kg时的平均加样回收率为85.6 ~ 93.5%,精密度为1.7 ~ 6.8%。本方法的定量限为0.01 mg/kg。结果表明,该方法可用于畜产品中喹氯灭的分析。
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引用次数: 0
[Single-laboratory Validation of an Analytical Method for Detarmination of Fusarium toxins in Buckwheat and Job's Tears]. [荞麦和薏米中镰刀菌毒素脱毒分析方法的单一实验室验证]。
IF 0.2 4区 农林科学 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2022-01-01 DOI: 10.3358/shokueishi.63.117
Miho Kawanishi, Kazuya Suenaga, Tomohiro Hirano

Fusarium species infect the major cereals consumed as food and feed, contaminating them with various toxic secondary metabolites known as toxins. Among these toxins, which include trichothecenes, zearalenone (ZEA), and fumonisins, the type-B trichothecene deoxynivalenol (DON) is generally considered as the most important. The present study evaluates an analytical method for the detection and quantification of multiple Fusarium toxins, namely, DON, acetyl forms of DON (3-Ac-DON and 15-Ac-DON), a glycoside form of DON (DON-3G), and other Fusarium toxins (nivalenol, an acetyl form of NIV (fusarenonX), T-2 and HT-2 toxins, diacetoxyscirpenol, and ZEA) in Job's tears and buckwheat.

镰刀菌感染了作为食物和饲料食用的主要谷物,使其受到各种有毒次生代谢物(称为毒素)的污染。这些毒素包括单端孢霉烯、玉米赤霉烯酮(ZEA)和烟曲霉毒素,其中 B 型单端孢霉烯脱氧雪腐镰刀菌烯醇(DON)通常被认为是最重要的毒素。本研究评估了一种用于检测和定量薏米和荞麦中多种镰刀菌毒素的分析方法,即 DON、DON 的乙酰形式(3-Ac-DON 和 15-Ac-DON)、DON 的糖苷形式(DON-3G)以及其他镰刀菌毒素(新戊烯醇、NIV 的乙酰形式(fusarenonX)、T-2 和 HT-2 毒素、二乙酰氧基藨草酚和 ZEA)。
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引用次数: 0
[Detection of Tetrodotoxin in Vomit and Urine from a Patient Suffering from Pufferfish Poisoning]. [一例河豚中毒患者呕吐物和尿液中河豚毒素的检测]。
IF 0.3 4区 农林科学 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2022-01-01 DOI: 10.3358/shokueishi.63.225
Junko Namba, Toyohiro Urayama, Hidefumi Kaneko, Atsushi Sato, Kae Fujimoto, Noriko Shigeta

We experienced a pufferfish poisoning case where no food residue was available to detect a causative agent. However, tetrodotoxin (TTX) was detected in vomit and urine samples from a patient using LC-MS/MS. Furthermore, we found a significant matrix effect in this analysis, indicating that the retention time of vomit and urine was not identical to the TTX standard solution and measured values multiplied by the dilution factors were not constant. Elimination of this matrix effect was attained by dilution of samples based on the retention time of the TTX standard solution, i.e., 10-time dilution of vomit test sample for LC-MS/MS analysis or 100-200-time dilution of urine one. Further research on urine analytical methods revealed that when TTX concentrations were too low to identify its peak on a chromatogram, TTX could be identified through a dilution procedure. It also showed that the application of the matrix-added TTX standard solution was effective for quantitative analysis under the influence of the matrix.

我们经历了一个河豚中毒的案例,没有食物残渣来检测病原体。然而,使用LC-MS/MS检测患者呕吐物和尿液样本中的河豚毒素(TTX)。此外,我们在本分析中发现了显著的基质效应,表明呕吐物和尿液的保留时间与TTX标准溶液不相同,测量值乘以稀释系数也不是恒定的。根据TTX标准溶液的保留时间对样品进行稀释,即将呕吐物样品稀释10倍用于LC-MS/MS分析,或将尿液样品稀释100-200倍,以消除这种基质效应。对尿液分析方法的进一步研究表明,当TTX浓度过低而无法在色谱图上识别其峰时,可以通过稀释程序识别TTX。实验还表明,在基质的影响下,添加基质的TTX标准溶液对定量分析是有效的。
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引用次数: 0
[Quantitative Analysis of Bisacurone in Turmeric by HPLC Using Relative Molar Sensitivity]. 姜黄中比沙酮的相对摩尔灵敏度高效液相色谱定量分析
IF 0.3 4区 农林科学 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2022-01-01 DOI: 10.3358/shokueishi.63.202
Kazuo Koyama, Hiroshi Sasako, Yohei Higashi, Hirono Ichikawa, Ayako Nagoya, Takashi Hirao

A novel method was developed for quantification of bisacuron (BC) and dehydrozingerone (DZ), the functional component of turmeric (Curcuma longa.L)-containing foods, using a relative molar sensitivity (RMS) method based on the combination of HPLC-UV and 1H-NMR. The RMSs of BC and DZ using 4-hydroxybenzoic acid ethyl ester (HBE) as the internal standard were calculated to 1.66 and 2.55, respectively. Analysis of fourteen beverage products showed the high correlations between the concentrations of BC and DZ quantified by the RMS method and those quantified by absolute calibration curve method. A collaborative study was conducted by four laboratories on one beverage and one tablet products. The repeatable relative standard deviation (RSDr) of intra-laboratories ranged from 0.7 to 1.7%, and the reproducible relative standard deviation (RSDR) of inter-laboratories ranged from 2.0 to 7.3%. The RMS method enabled the quantification of analytes for which difficultly obtain standard materials such as BC and DZ, using an internal standard for which obtain routinely readily available. This RMS method is expected to be applied to quality control for food products containing turmeric.

建立了一种基于HPLC-UV和1H-NMR相结合的相对摩尔灵敏度(RMS)法测定含姜黄(Curcuma longa.L)食品中功能成分比沙库隆(BC)和脱氢姜酮(DZ)含量的新方法。以4-羟基苯甲酸乙酯(HBE)为内标,计算BC和DZ的均方根偏差分别为1.66和2.55。对14种饮料产品的分析表明,均方根法测定的BC和DZ浓度与绝对校准曲线法测定的BC和DZ浓度具有较高的相关性。四个实验室对一种饮料和一种片剂产品进行了合作研究。实验室内的可重复相对标准偏差(RSDr)为0.7 ~ 1.7%,实验室间的可重复相对标准偏差(RSDr)为2.0 ~ 7.3%。RMS方法可以对BC和DZ等难以获得标准物质的分析物进行定量,使用常规容易获得的内标。该方法有望应用于含姜黄食品的质量控制。
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引用次数: 0
[Prevalence of Foodborne Pathogens in Retail Meat in Saitama, Japan]. [日本埼玉市零售肉类中食源性病原体的流行情况]。
IF 0.3 4区 农林科学 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2022-01-01 DOI: 10.3358/shokueishi.63.151
Nozomi Sakakida, Mika Sato, Satomi Kando, Kaori Kashima, Shinichi Shimada, Rie Ishii

We investigated the prevalence of Campylobacter jejuni, Campylobacter coli, Salmonella, enterohemorrhagic Escherichia coli, enterotoxigenic Escherichia coli, Yersinia enterocolitica, and Escherichia albertii in domestic chicken and pork sold at retail stores in Saitama Prefecture, Japan. Campylobacter was detected in 35.7% (60/168) of chicken samples and 7.3% (14/190) of pork samples. C. jejuni and C. coli were predominant in chicken and pork, respectively. Salmonella was found in 58.1% (100/172) of chicken samples and 19.9% (41/206) of pork samples. Moreover, Salmonella Schwarzengrund was the major serovar observed in chicken isolates, whereas S. Typhimurium monophasic variant was in pork isolates. Furthermore, ETEC was found in 0.6% (1/160) of chicken samples and 2.4% (5/206) of pork samples. Y. enterocolitica was absent from all (83/83) chicken samples but was present in 9.3% (18/193) of pork samples, with a prevalence in pork tongues as high as 21.0% (13/62 samples). However, EHEC and E. albertii were not detected in our study. Therefore, the results of this study indicate that chicken was highly contaminated with Campylobacter sp. and Salmonella, and pork was with Y. enterocolitica serotype O3, Campylobacter sp., Salmonella, and ETEC.

我们调查了空肠弯曲杆菌、大肠弯曲杆菌、沙门氏菌、肠出血性大肠埃希菌、产肠毒素大肠埃希菌、小肠结肠炎耶尔森菌和阿尔伯蒂埃希菌在日本埼玉县零售商店销售的家鸡和猪肉中的流行情况。鸡肉和猪肉的弯曲杆菌检出率分别为35.7%(60/168)和7.3%(14/190)。鸡肉和猪肉中分别以空肠梭菌和大肠杆菌为主。检出沙门氏菌的鸡肉样品占58.1%(100/172),猪肉样品占19.9%(41/206)。此外,在鸡分离株中观察到的主要血清型是萧氏沙门氏菌,而在猪肉分离株中观察到的主要是单相鼠伤寒沙门氏菌变体。鸡肉和猪肉中分别有0.6%(1/160)和2.4%(5/206)存在ETEC。所有鸡肉样本(83/83)中未检出小肠结肠炎耶氏菌,但9.3%(18/193)的猪肉样本中检出该菌,其中猪舌的检出率高达21.0%(13/62)。然而,在我们的研究中未检测到肠出血性大肠杆菌和阿尔伯蒂大肠杆菌。因此,本研究结果表明,鸡肉受到弯曲杆菌和沙门氏菌的高度污染,猪肉受到O3型小肠结肠炎耶氏菌、弯曲杆菌、沙门氏菌和ETEC的严重污染。
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引用次数: 0
[Survey of Arsenic/Heavy Metals and Pesticide Residues in Edible Insects for Human Consumption or Supplied in Japan]. [日本供人食用或供应的食用昆虫中砷/重金属及农药残留调查]。
IF 0.3 4区 农林科学 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2022-01-01 DOI: 10.3358/shokueishi.63.136
Yugo Kitahara, Hiroshi Nomura, Nanami Nishihara, Takuya Ueda, Satoru Watanabe, Isao Saito, Jun Ueyama

Arsenic (As) and heavy metals (Cd, Hg, Pb, and Cu) and pesticide residues in 14 edible insects were investigated. The maximum levels of elements were 6.15 for As, 0.82 for Cd, 0.50 for Hg, 0.67 for Pb, and 297.7 ppm for Cu. Fenobucarb (or BPMC) has been quantified through GC- and LC-MS/MS analysis at a concentration of approximately 3 ppm. Further studies of the contaminants may help ensure the safety of edible insect consumption.

对14种食用昆虫的砷(As)、重金属(Cd、Hg、Pb、Cu)和农药残留进行了调查。砷的最大含量为6.15 ppm,镉为0.82 ppm,汞为0.50 ppm,铅为0.67 ppm,铜为297.7 ppm。通过GC-和LC-MS/MS分析,在大约3ppm的浓度下对灭虫威(或BPMC)进行了定量。对污染物的进一步研究可能有助于确保食用昆虫的安全。
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引用次数: 1
[Separate Analysis of Loquat Fruit Pulp, Peel, and Pits to Calculate Pesticide Residue Levels in the Whole Commodity]. [分别分析枇杷果肉、果皮和果核以计算整个商品中的农药残留量]。
IF 0.2 4区 农林科学 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2022-01-01 DOI: 10.3358/shokueishi.63.62
Shinobu Hikino, Tomonari Yajima, Michiko Sakasai, Naruto Tomiyama, Kazuaki Iijima, Kazutoshi Ohyama

To evaluate the effects of handling "not detectable" residues (ND: <0.01 mg/kg) in the pulp and detectable residues in the pits on the calculation of pesticide residue in the whole fruit, residue levels in the pulp, peel, and pits of loquat fruits were separately analyzed. Following conventional Japanese agricultural practices, 16 pesticides were sprayed at the maximum application rates in three test fields. All target pesticides were detected at quantifiable levels in the peel (n=144). In contrast, the percentages of detected pesticides in the pulp and pits were 42% (n=61) and 36% (n=52), respectively. Most pesticide residues were present in the peel. For comparison, the pesticide residue levels in the whole fruits were determined based on three indices: the highest estimate (H), calculated using the measured residue levels in the pits and by replacing the ND residues in the pulp as the limit of quantification (LOQ) values; conventional estimate (C), calculated by neglecting all residues in the pits (0 mg/kg) and replacing the ND residues in the pulp as LOQ values; and the lowest estimate (L), calculated by neglecting all residues in the pits and the ND residues in the pulp (0 mg/kg). The L/C and H/C ratios ranged from 74% (L/C) to 106% (H/C). In seven of eighty-three cases with less than 90% difference, residue levels in the whole loquat fruits were low (≤0.06 mg/kg), with the actual range being equal to or below the minimum unit of 0.01. In comparison of three field datasets, the range of residue levels was estimated to be 2.77 mg/kg. Based on the results of separate analysis, handling of ND residues in the pulp and detectable residues in the pits did not significantly affect the calculated pesticide residue levels in the whole loquat fruits.

为了评估处理 "检测不到 "残留物(ND.ND:
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引用次数: 0
[Simultaneous Analysis of 7 Antiviral Agents in Chicken Tissues and Processed Products by LC-MS/MS]. [利用 LC-MS/MS 同时分析鸡组织和加工产品中的 7 种抗病毒药物]。
IF 0.2 4区 农林科学 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2022-01-01 DOI: 10.3358/shokueishi.63.1
Takayuki Asakura, Mariko Kitamura, Miho Yasumoto, Yoshitaka Takeuchi, Mitsuo Nakazato, Kazuo Yasuda

Since amantadine, rimantadine, arbidol, laninamvir, oseltamivir, peramivir, and zanamivir may be used as antiviral agents to treat avian influenza, we herein developed a simultaneous assay using LC-MS/MS. This method was applied to chicken products (including yakitori (grilled chicken), fried chicken, chicken steak, and boiled eggs) as well as chicken tissues (muscle, fat, the liver, gizzards, and heart) and eggs.Samples were extracted with methanol-water (9 : 1), purified by a tandem column with an InertSep® MAX cartridge (upper part) and InertSep® MCX cartridge (lower part), and then measured by LC-MS/MS. The sample matrix had no effect on the identification of compounds. Chromatographic separation was performed on a ZIC-HILIC column using a mobile phase of 1% acetic acid solution and 1% acetic acid solution in acetonitrile, resulting in complete separation and other obstructive peaks from the sample matrices. An external solvent calibration curve was used for quantification.The application of the method to 6 samples of chicken tissues and eggs achieved good results of between 77.9 and 97.5% for trueness and between 1.7 and 9.2% for concurrent accuracy. The method was also applied to 9 samples of processed products, including grilled chicken and fried chicken, and achieved good results with true percentages ranging between 72.6 and 99.2% and concurrent accuracies between 3.0 and 11.2%. Therefore, the developed method may also be applied to processed products.The limit of quantification (LOQ) of the developed method was 0.01 mg/kg.The method was then applied to 42 types of commercial processed products, including yakitori, fried chicken, steamed chicken, chicken steak, and boiled eggs, and no antiviral agents were detected.Collectively, the present results confirmed that the method developed herein is applicable to not only chicken tissues, but also their processed products.

由于金刚烷胺、利曼他定、阿比多、拉尼那韦、奥司他韦、帕拉米韦和扎那米韦可能被用作治疗禽流感的抗病毒药物,我们在此开发了一种使用 LC-MS/MS 的同步检测方法。样品经甲醇-水(9:1)提取,用 InertSep® MAX 色谱柱(上部)和 InertSep® MCX 色谱柱(下部)串联净化,然后用 LC-MS/MS 检测。样品基质对化合物的鉴定没有影响。色谱分离在 ZIC-HILIC 色谱柱上进行,流动相为 1%乙酸溶液和乙腈中的 1%乙酸溶液,结果完全分离了样品基质中的其他阻碍峰。对 6 份鸡组织和鸡蛋样品进行了分析,结果表明该方法的准确度在 77.9% 至 97.5% 之间,同时准确度在 1.7% 至 9.2% 之间。该方法还应用于包括烤鸡和炸鸡在内的 9 个加工产品样品,并取得了良好的结果,真实率介于 72.6% 和 99.2% 之间,并发准确率介于 3.0% 和 11.2% 之间。该方法的定量限(LOQ)为0.01 mg/kg。然后将该方法应用于42种商业加工产品,包括烤鸡肉串、炸鸡、蒸鸡、鸡排和煮鸡蛋,均未检出抗病毒剂。
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引用次数: 0
[Pharmaceutical Ingredients Detected in Dietary Supplements Purchased by Chiba Prefecture (Fiscal Years 2014-2021)]. 【千叶县购买的膳食补充剂中检出的药物成分(2014-2021财年)】。
IF 0.3 4区 农林科学 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2022-01-01 DOI: 10.3358/shokueishi.63.141
Tomohide Fukiwake, Keigo Enomoto, Hiroki Yoshino, Katsuya Uchimoto, Maki Nishimura, Hiroshi Akiyama

In the pharmaceutical ingredients contamination testing of 702 commercial dietary supplement products, during fiscal years 2014-2021, 14 pharmaceutical ingredients, barrenwort, leaf axils of senna, and small leaf of senna were detected in 28 products. Screening and confirmation of the pharmaceutical ingredients in the products were performed by ultra-high performance liquid chromatography with photodiode array detector and ultra-high performance liquid chromatography-quadrupole-kingdon trap mass spectrometry, respectively. In particular, leaf axils and small leaf of senna were identified by stereomicroscopy and scanning electron microscopy. Furthermore, we found several pharmaceutical ingredients that exceeded the daily medicated dosage; therefore, it is important to prevent the distribution of such products to prevent the occurrence of health hazard. For that reason, it is necessary to continue the sample purchasing and testing systems to monitor the distribution of products containing pharmaceutical ingredients.

在2014-2021财年702种市售膳食补充剂产品的药物成分污染检测中,在28种产品中检测出14种药物成分、无毛草、番泻叶叶腋、番泻叶小叶。分别采用光电二极管阵列超高效液相色谱法和超高效液相色谱-四极杆-金顿阱质谱法对产品中的药物成分进行筛选和鉴定。利用体视显微镜和扫描电镜对番泻叶的叶腋和小叶进行了鉴定。此外,我们发现一些药物成分超过了日常用药剂量;因此,防止此类产品的流通对防止健康危害的发生至关重要。因此,有必要继续实施样品采购和检测系统,以监测含有药物成分的产品的分销。
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引用次数: 0
期刊
Food Hygiene and Safety Science
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