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[Detection of Tetrodotoxin in Vomit and Urine from a Patient Suffering from Pufferfish Poisoning]. [一例河豚中毒患者呕吐物和尿液中河豚毒素的检测]。
IF 0.3 4区 农林科学 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2022-01-01 DOI: 10.3358/shokueishi.63.225
Junko Namba, Toyohiro Urayama, Hidefumi Kaneko, Atsushi Sato, Kae Fujimoto, Noriko Shigeta

We experienced a pufferfish poisoning case where no food residue was available to detect a causative agent. However, tetrodotoxin (TTX) was detected in vomit and urine samples from a patient using LC-MS/MS. Furthermore, we found a significant matrix effect in this analysis, indicating that the retention time of vomit and urine was not identical to the TTX standard solution and measured values multiplied by the dilution factors were not constant. Elimination of this matrix effect was attained by dilution of samples based on the retention time of the TTX standard solution, i.e., 10-time dilution of vomit test sample for LC-MS/MS analysis or 100-200-time dilution of urine one. Further research on urine analytical methods revealed that when TTX concentrations were too low to identify its peak on a chromatogram, TTX could be identified through a dilution procedure. It also showed that the application of the matrix-added TTX standard solution was effective for quantitative analysis under the influence of the matrix.

我们经历了一个河豚中毒的案例,没有食物残渣来检测病原体。然而,使用LC-MS/MS检测患者呕吐物和尿液样本中的河豚毒素(TTX)。此外,我们在本分析中发现了显著的基质效应,表明呕吐物和尿液的保留时间与TTX标准溶液不相同,测量值乘以稀释系数也不是恒定的。根据TTX标准溶液的保留时间对样品进行稀释,即将呕吐物样品稀释10倍用于LC-MS/MS分析,或将尿液样品稀释100-200倍,以消除这种基质效应。对尿液分析方法的进一步研究表明,当TTX浓度过低而无法在色谱图上识别其峰时,可以通过稀释程序识别TTX。实验还表明,在基质的影响下,添加基质的TTX标准溶液对定量分析是有效的。
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引用次数: 0
[Quantitative Analysis of Bisacurone in Turmeric by HPLC Using Relative Molar Sensitivity]. 姜黄中比沙酮的相对摩尔灵敏度高效液相色谱定量分析
IF 0.3 4区 农林科学 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2022-01-01 DOI: 10.3358/shokueishi.63.202
Kazuo Koyama, Hiroshi Sasako, Yohei Higashi, Hirono Ichikawa, Ayako Nagoya, Takashi Hirao

A novel method was developed for quantification of bisacuron (BC) and dehydrozingerone (DZ), the functional component of turmeric (Curcuma longa.L)-containing foods, using a relative molar sensitivity (RMS) method based on the combination of HPLC-UV and 1H-NMR. The RMSs of BC and DZ using 4-hydroxybenzoic acid ethyl ester (HBE) as the internal standard were calculated to 1.66 and 2.55, respectively. Analysis of fourteen beverage products showed the high correlations between the concentrations of BC and DZ quantified by the RMS method and those quantified by absolute calibration curve method. A collaborative study was conducted by four laboratories on one beverage and one tablet products. The repeatable relative standard deviation (RSDr) of intra-laboratories ranged from 0.7 to 1.7%, and the reproducible relative standard deviation (RSDR) of inter-laboratories ranged from 2.0 to 7.3%. The RMS method enabled the quantification of analytes for which difficultly obtain standard materials such as BC and DZ, using an internal standard for which obtain routinely readily available. This RMS method is expected to be applied to quality control for food products containing turmeric.

建立了一种基于HPLC-UV和1H-NMR相结合的相对摩尔灵敏度(RMS)法测定含姜黄(Curcuma longa.L)食品中功能成分比沙库隆(BC)和脱氢姜酮(DZ)含量的新方法。以4-羟基苯甲酸乙酯(HBE)为内标,计算BC和DZ的均方根偏差分别为1.66和2.55。对14种饮料产品的分析表明,均方根法测定的BC和DZ浓度与绝对校准曲线法测定的BC和DZ浓度具有较高的相关性。四个实验室对一种饮料和一种片剂产品进行了合作研究。实验室内的可重复相对标准偏差(RSDr)为0.7 ~ 1.7%,实验室间的可重复相对标准偏差(RSDr)为2.0 ~ 7.3%。RMS方法可以对BC和DZ等难以获得标准物质的分析物进行定量,使用常规容易获得的内标。该方法有望应用于含姜黄食品的质量控制。
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引用次数: 0
[Prevalence of Foodborne Pathogens in Retail Meat in Saitama, Japan]. [日本埼玉市零售肉类中食源性病原体的流行情况]。
IF 0.3 4区 农林科学 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2022-01-01 DOI: 10.3358/shokueishi.63.151
Nozomi Sakakida, Mika Sato, Satomi Kando, Kaori Kashima, Shinichi Shimada, Rie Ishii

We investigated the prevalence of Campylobacter jejuni, Campylobacter coli, Salmonella, enterohemorrhagic Escherichia coli, enterotoxigenic Escherichia coli, Yersinia enterocolitica, and Escherichia albertii in domestic chicken and pork sold at retail stores in Saitama Prefecture, Japan. Campylobacter was detected in 35.7% (60/168) of chicken samples and 7.3% (14/190) of pork samples. C. jejuni and C. coli were predominant in chicken and pork, respectively. Salmonella was found in 58.1% (100/172) of chicken samples and 19.9% (41/206) of pork samples. Moreover, Salmonella Schwarzengrund was the major serovar observed in chicken isolates, whereas S. Typhimurium monophasic variant was in pork isolates. Furthermore, ETEC was found in 0.6% (1/160) of chicken samples and 2.4% (5/206) of pork samples. Y. enterocolitica was absent from all (83/83) chicken samples but was present in 9.3% (18/193) of pork samples, with a prevalence in pork tongues as high as 21.0% (13/62 samples). However, EHEC and E. albertii were not detected in our study. Therefore, the results of this study indicate that chicken was highly contaminated with Campylobacter sp. and Salmonella, and pork was with Y. enterocolitica serotype O3, Campylobacter sp., Salmonella, and ETEC.

我们调查了空肠弯曲杆菌、大肠弯曲杆菌、沙门氏菌、肠出血性大肠埃希菌、产肠毒素大肠埃希菌、小肠结肠炎耶尔森菌和阿尔伯蒂埃希菌在日本埼玉县零售商店销售的家鸡和猪肉中的流行情况。鸡肉和猪肉的弯曲杆菌检出率分别为35.7%(60/168)和7.3%(14/190)。鸡肉和猪肉中分别以空肠梭菌和大肠杆菌为主。检出沙门氏菌的鸡肉样品占58.1%(100/172),猪肉样品占19.9%(41/206)。此外,在鸡分离株中观察到的主要血清型是萧氏沙门氏菌,而在猪肉分离株中观察到的主要是单相鼠伤寒沙门氏菌变体。鸡肉和猪肉中分别有0.6%(1/160)和2.4%(5/206)存在ETEC。所有鸡肉样本(83/83)中未检出小肠结肠炎耶氏菌,但9.3%(18/193)的猪肉样本中检出该菌,其中猪舌的检出率高达21.0%(13/62)。然而,在我们的研究中未检测到肠出血性大肠杆菌和阿尔伯蒂大肠杆菌。因此,本研究结果表明,鸡肉受到弯曲杆菌和沙门氏菌的高度污染,猪肉受到O3型小肠结肠炎耶氏菌、弯曲杆菌、沙门氏菌和ETEC的严重污染。
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引用次数: 0
[Survey of Arsenic/Heavy Metals and Pesticide Residues in Edible Insects for Human Consumption or Supplied in Japan]. [日本供人食用或供应的食用昆虫中砷/重金属及农药残留调查]。
IF 0.3 4区 农林科学 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2022-01-01 DOI: 10.3358/shokueishi.63.136
Yugo Kitahara, Hiroshi Nomura, Nanami Nishihara, Takuya Ueda, Satoru Watanabe, Isao Saito, Jun Ueyama

Arsenic (As) and heavy metals (Cd, Hg, Pb, and Cu) and pesticide residues in 14 edible insects were investigated. The maximum levels of elements were 6.15 for As, 0.82 for Cd, 0.50 for Hg, 0.67 for Pb, and 297.7 ppm for Cu. Fenobucarb (or BPMC) has been quantified through GC- and LC-MS/MS analysis at a concentration of approximately 3 ppm. Further studies of the contaminants may help ensure the safety of edible insect consumption.

对14种食用昆虫的砷(As)、重金属(Cd、Hg、Pb、Cu)和农药残留进行了调查。砷的最大含量为6.15 ppm,镉为0.82 ppm,汞为0.50 ppm,铅为0.67 ppm,铜为297.7 ppm。通过GC-和LC-MS/MS分析,在大约3ppm的浓度下对灭虫威(或BPMC)进行了定量。对污染物的进一步研究可能有助于确保食用昆虫的安全。
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引用次数: 1
[Separate Analysis of Loquat Fruit Pulp, Peel, and Pits to Calculate Pesticide Residue Levels in the Whole Commodity]. [分别分析枇杷果肉、果皮和果核以计算整个商品中的农药残留量]。
IF 0.2 4区 农林科学 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2022-01-01 DOI: 10.3358/shokueishi.63.62
Shinobu Hikino, Tomonari Yajima, Michiko Sakasai, Naruto Tomiyama, Kazuaki Iijima, Kazutoshi Ohyama

To evaluate the effects of handling "not detectable" residues (ND: <0.01 mg/kg) in the pulp and detectable residues in the pits on the calculation of pesticide residue in the whole fruit, residue levels in the pulp, peel, and pits of loquat fruits were separately analyzed. Following conventional Japanese agricultural practices, 16 pesticides were sprayed at the maximum application rates in three test fields. All target pesticides were detected at quantifiable levels in the peel (n=144). In contrast, the percentages of detected pesticides in the pulp and pits were 42% (n=61) and 36% (n=52), respectively. Most pesticide residues were present in the peel. For comparison, the pesticide residue levels in the whole fruits were determined based on three indices: the highest estimate (H), calculated using the measured residue levels in the pits and by replacing the ND residues in the pulp as the limit of quantification (LOQ) values; conventional estimate (C), calculated by neglecting all residues in the pits (0 mg/kg) and replacing the ND residues in the pulp as LOQ values; and the lowest estimate (L), calculated by neglecting all residues in the pits and the ND residues in the pulp (0 mg/kg). The L/C and H/C ratios ranged from 74% (L/C) to 106% (H/C). In seven of eighty-three cases with less than 90% difference, residue levels in the whole loquat fruits were low (≤0.06 mg/kg), with the actual range being equal to or below the minimum unit of 0.01. In comparison of three field datasets, the range of residue levels was estimated to be 2.77 mg/kg. Based on the results of separate analysis, handling of ND residues in the pulp and detectable residues in the pits did not significantly affect the calculated pesticide residue levels in the whole loquat fruits.

为了评估处理 "检测不到 "残留物(ND.ND:
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引用次数: 0
[Simultaneous Analysis of 7 Antiviral Agents in Chicken Tissues and Processed Products by LC-MS/MS]. [利用 LC-MS/MS 同时分析鸡组织和加工产品中的 7 种抗病毒药物]。
IF 0.2 4区 农林科学 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2022-01-01 DOI: 10.3358/shokueishi.63.1
Takayuki Asakura, Mariko Kitamura, Miho Yasumoto, Yoshitaka Takeuchi, Mitsuo Nakazato, Kazuo Yasuda

Since amantadine, rimantadine, arbidol, laninamvir, oseltamivir, peramivir, and zanamivir may be used as antiviral agents to treat avian influenza, we herein developed a simultaneous assay using LC-MS/MS. This method was applied to chicken products (including yakitori (grilled chicken), fried chicken, chicken steak, and boiled eggs) as well as chicken tissues (muscle, fat, the liver, gizzards, and heart) and eggs.Samples were extracted with methanol-water (9 : 1), purified by a tandem column with an InertSep® MAX cartridge (upper part) and InertSep® MCX cartridge (lower part), and then measured by LC-MS/MS. The sample matrix had no effect on the identification of compounds. Chromatographic separation was performed on a ZIC-HILIC column using a mobile phase of 1% acetic acid solution and 1% acetic acid solution in acetonitrile, resulting in complete separation and other obstructive peaks from the sample matrices. An external solvent calibration curve was used for quantification.The application of the method to 6 samples of chicken tissues and eggs achieved good results of between 77.9 and 97.5% for trueness and between 1.7 and 9.2% for concurrent accuracy. The method was also applied to 9 samples of processed products, including grilled chicken and fried chicken, and achieved good results with true percentages ranging between 72.6 and 99.2% and concurrent accuracies between 3.0 and 11.2%. Therefore, the developed method may also be applied to processed products.The limit of quantification (LOQ) of the developed method was 0.01 mg/kg.The method was then applied to 42 types of commercial processed products, including yakitori, fried chicken, steamed chicken, chicken steak, and boiled eggs, and no antiviral agents were detected.Collectively, the present results confirmed that the method developed herein is applicable to not only chicken tissues, but also their processed products.

由于金刚烷胺、利曼他定、阿比多、拉尼那韦、奥司他韦、帕拉米韦和扎那米韦可能被用作治疗禽流感的抗病毒药物,我们在此开发了一种使用 LC-MS/MS 的同步检测方法。样品经甲醇-水(9:1)提取,用 InertSep® MAX 色谱柱(上部)和 InertSep® MCX 色谱柱(下部)串联净化,然后用 LC-MS/MS 检测。样品基质对化合物的鉴定没有影响。色谱分离在 ZIC-HILIC 色谱柱上进行,流动相为 1%乙酸溶液和乙腈中的 1%乙酸溶液,结果完全分离了样品基质中的其他阻碍峰。对 6 份鸡组织和鸡蛋样品进行了分析,结果表明该方法的准确度在 77.9% 至 97.5% 之间,同时准确度在 1.7% 至 9.2% 之间。该方法还应用于包括烤鸡和炸鸡在内的 9 个加工产品样品,并取得了良好的结果,真实率介于 72.6% 和 99.2% 之间,并发准确率介于 3.0% 和 11.2% 之间。该方法的定量限(LOQ)为0.01 mg/kg。然后将该方法应用于42种商业加工产品,包括烤鸡肉串、炸鸡、蒸鸡、鸡排和煮鸡蛋,均未检出抗病毒剂。
{"title":"[Simultaneous Analysis of 7 Antiviral Agents in Chicken Tissues and Processed Products by LC-MS/MS].","authors":"Takayuki Asakura, Mariko Kitamura, Miho Yasumoto, Yoshitaka Takeuchi, Mitsuo Nakazato, Kazuo Yasuda","doi":"10.3358/shokueishi.63.1","DOIUrl":"10.3358/shokueishi.63.1","url":null,"abstract":"<p><p>Since amantadine, rimantadine, arbidol, laninamvir, oseltamivir, peramivir, and zanamivir may be used as antiviral agents to treat avian influenza, we herein developed a simultaneous assay using LC-MS/MS. This method was applied to chicken products (including yakitori (grilled chicken), fried chicken, chicken steak, and boiled eggs) as well as chicken tissues (muscle, fat, the liver, gizzards, and heart) and eggs.Samples were extracted with methanol-water (9 : 1), purified by a tandem column with an InertSep<sup>®</sup> MAX cartridge (upper part) and InertSep<sup>®</sup> MCX cartridge (lower part), and then measured by LC-MS/MS. The sample matrix had no effect on the identification of compounds. Chromatographic separation was performed on a ZIC-HILIC column using a mobile phase of 1% acetic acid solution and 1% acetic acid solution in acetonitrile, resulting in complete separation and other obstructive peaks from the sample matrices. An external solvent calibration curve was used for quantification.The application of the method to 6 samples of chicken tissues and eggs achieved good results of between 77.9 and 97.5% for trueness and between 1.7 and 9.2% for concurrent accuracy. The method was also applied to 9 samples of processed products, including grilled chicken and fried chicken, and achieved good results with true percentages ranging between 72.6 and 99.2% and concurrent accuracies between 3.0 and 11.2%. Therefore, the developed method may also be applied to processed products.The limit of quantification (LOQ) of the developed method was 0.01 mg/kg.The method was then applied to 42 types of commercial processed products, including yakitori, fried chicken, steamed chicken, chicken steak, and boiled eggs, and no antiviral agents were detected.Collectively, the present results confirmed that the method developed herein is applicable to not only chicken tissues, but also their processed products.</p>","PeriodicalId":54373,"journal":{"name":"Food Hygiene and Safety Science","volume":"1 1","pages":"1-11"},"PeriodicalIF":0.2,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77084603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Pharmaceutical Ingredients Detected in Dietary Supplements Purchased by Chiba Prefecture (Fiscal Years 2014-2021)]. 【千叶县购买的膳食补充剂中检出的药物成分(2014-2021财年)】。
IF 0.3 4区 农林科学 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2022-01-01 DOI: 10.3358/shokueishi.63.141
Tomohide Fukiwake, Keigo Enomoto, Hiroki Yoshino, Katsuya Uchimoto, Maki Nishimura, Hiroshi Akiyama

In the pharmaceutical ingredients contamination testing of 702 commercial dietary supplement products, during fiscal years 2014-2021, 14 pharmaceutical ingredients, barrenwort, leaf axils of senna, and small leaf of senna were detected in 28 products. Screening and confirmation of the pharmaceutical ingredients in the products were performed by ultra-high performance liquid chromatography with photodiode array detector and ultra-high performance liquid chromatography-quadrupole-kingdon trap mass spectrometry, respectively. In particular, leaf axils and small leaf of senna were identified by stereomicroscopy and scanning electron microscopy. Furthermore, we found several pharmaceutical ingredients that exceeded the daily medicated dosage; therefore, it is important to prevent the distribution of such products to prevent the occurrence of health hazard. For that reason, it is necessary to continue the sample purchasing and testing systems to monitor the distribution of products containing pharmaceutical ingredients.

在2014-2021财年702种市售膳食补充剂产品的药物成分污染检测中,在28种产品中检测出14种药物成分、无毛草、番泻叶叶腋、番泻叶小叶。分别采用光电二极管阵列超高效液相色谱法和超高效液相色谱-四极杆-金顿阱质谱法对产品中的药物成分进行筛选和鉴定。利用体视显微镜和扫描电镜对番泻叶的叶腋和小叶进行了鉴定。此外,我们发现一些药物成分超过了日常用药剂量;因此,防止此类产品的流通对防止健康危害的发生至关重要。因此,有必要继续实施样品采购和检测系统,以监测含有药物成分的产品的分销。
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引用次数: 0
[A Rapid Determination Method of Metals in Foods for Emergency Response to Health Crisis]. 食品中金属的快速测定方法及其对健康危机的应急响应
IF 0.3 4区 农林科学 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2022-01-01 DOI: 10.3358/shokueishi.63.105
Kyoko Takagi, Hitoshi Miyazaki

A rapid determination method for emergency response to health crisis caused by metals in foods, was developed using microwave decomposition equipment and inductively coupled plasma atomic emission spectroscopy (ICP-AES). The method was assessed for 18 elements (Al, As, B, Cd, Co, Cr, Cu, Fe, Hg, Mn, Mo, Ni, Pb, Sb, Se, Sn, Tl and Zn) in 5 kinds of beverages and 7 kinds of foods. A single-laboratory method validation study was performed using food samples added with 20 mg/kg of each metal. Trueness was 88-108% and intralaboratory reproducibility was 0.2-11.3%. Time required for analysis was less than 3 hr. Thus, the presented method could be useful for rapid analysis of metals involved food poisoning cases.

采用微波分解装置和电感耦合等离子体原子发射光谱(ICP-AES)技术,建立了食品中金属污染健康危机应急快速检测方法。采用该方法对5种饮料和7种食品中的18种元素(Al、As、B、Cd、Co、Cr、Cu、Fe、Hg、Mn、Mo、Ni、Pb、Sb、Se、Sn、Tl、Zn)进行了测定。使用添加每种金属20 mg/kg的食品样品进行了单实验室方法验证研究。准确度为88 ~ 108%,实验室内重现性为0.2 ~ 11.3%。分析所需时间小于3小时。因此,该方法可用于食物中毒案件中金属元素的快速分析。
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引用次数: 0
[Understanding and Attitudes of Dietitians towards "Foods with Function Claims"]. [营养师对“有功能声称的食物”的认识及态度]。
IF 0.3 4区 农林科学 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2021-01-01 DOI: 10.3358/shokueishi.62.20
Chiharu Nishijima, Kaoru Sato, Tsuyoshi Chiba
The role of dietitians is important for consumers to practice self-care, which includes the use of the "Foods with Function Claims (FFC)". In this study, a nationwide internet survey was conducted to clarify the understanding and attitudes of dietitians towards the FFC 1 year and 4 years after its introduction (Surveys were conducted in 2015 and 2019, respectively). In the survey of 1 year after the introduction, the ratio of recognition of the FFC was 98%; however, only 35% correctly understood the characteristics of the FFC. Similarly, in the survey of 4 year after the introduction, only 42% of dietitians correctly answered the characteristics. At 4 year after, 56% of dietitians currently or previously have used the FFC. The dietitians who had been consulted about the use of the FFC accounted for 22% and about the adverse events accounted for 15%. The advice they gave at the consultation differed according to their experience of the use of the FFC. Of the ones who never used the FFC, 25% referred no information sources for the FFC. The place to educate primarily working dietitians needs to be prepared to let them provide appropriate information to consumers to support self-care including the appropriate use of the FFC.
营养师的角色对消费者的自我护理很重要,其中包括使用“功能声明食品”。本研究在全国范围内进行了一项网络调查,以明确营养师对FFC引入1年和4年后的理解和态度(调查分别于2015年和2019年进行)。在引进后1年的调查中,FFC的认可率为98%;然而,只有35%的人正确理解FFC的特征。同样,在引进后4年的调查中,只有42%的营养师正确回答了这些特征。4年后,56%的营养师现在或以前使用FFC。咨询过FFC使用情况的营养师占22%,咨询过不良事件的营养师占15%。他们在谘询时所提供的意见,根据他们使用FFC的经验而有所不同。在那些从未使用过FFC的人中,25%的人没有提到FFC的信息来源。教育主要工作营养师的地方需要做好准备,让他们向消费者提供适当的信息,以支持自我保健,包括适当使用FFC。
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引用次数: 0
[Contamination of Pathogenic Escherichia coli in Retail Fresh Vegetables in the Mekong Delta, Vietnam]. [越南湄公河三角洲零售新鲜蔬菜中致病性大肠杆菌的污染]。
IF 0.3 4区 农林科学 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2021-01-01 DOI: 10.3358/shokueishi.62.94
Shunsuke Ikeuchi, Bui Thi Hien, Nguyen Khanh Thuan, Ly Thi, Yukiko Hara-Kudo, Takahide Taniguchi, Hideki Hayashidani

From July 2017 to January 2019, total of 645 retail fresh vegetables collected from 19 retail shops and markets was investigated to know the contamination of enterohemorrhagic Escherichia coli (EHEC) and enterotoxigenic E. coli (ETEC). Of 645 samples, 2 samples (0.3%) were positive for pathogenic E. coli. Of 2 pathogenic E. coli positive samples, 1 was EHEC (stx2 positive) and the other was ETEC (sta positive). Two pathogenic E. coli strains were isolated from crisphead lettuce. EHEC strain was not serotyped by commercial antisera and ETEC was serotyped as O20. EHEC and ETEC strains showed multi-drug resistance against 4 and 7 antibiotics, respectively. These results indicate that retail fresh vegetables seem to be not an important source of human EHEC and ETEC infection in the Mekong Delta, Vietnam.

2017年7月至2019年1月,对从19家零售商店和市场收集的645种零售新鲜蔬菜进行调查,了解肠出血性大肠杆菌(EHEC)和肠产毒素大肠杆菌(ETEC)的污染情况。645份样品中2份(0.3%)检出致病性大肠杆菌。2份致病性大肠杆菌阳性标本中,1份为EHEC (stx2阳性),1份为ETEC (sta阳性)。从脆头莴苣中分离到两株致病性大肠杆菌。市售抗血清未对EHEC菌株进行血清分型,ETEC血清分型为O20。EHEC和ETEC菌株分别对4种和7种抗生素耐药。这些结果表明,零售新鲜蔬菜似乎不是越南湄公河三角洲人类肠出血性大肠杆菌和肠出血性大肠杆菌感染的重要来源。
{"title":"[Contamination of Pathogenic Escherichia coli in Retail Fresh Vegetables in the Mekong Delta, Vietnam].","authors":"Shunsuke Ikeuchi,&nbsp;Bui Thi Hien,&nbsp;Nguyen Khanh Thuan,&nbsp;Ly Thi,&nbsp;Yukiko Hara-Kudo,&nbsp;Takahide Taniguchi,&nbsp;Hideki Hayashidani","doi":"10.3358/shokueishi.62.94","DOIUrl":"https://doi.org/10.3358/shokueishi.62.94","url":null,"abstract":"<p><p>From July 2017 to January 2019, total of 645 retail fresh vegetables collected from 19 retail shops and markets was investigated to know the contamination of enterohemorrhagic Escherichia coli (EHEC) and enterotoxigenic E. coli (ETEC). Of 645 samples, 2 samples (0.3%) were positive for pathogenic E. coli. Of 2 pathogenic E. coli positive samples, 1 was EHEC (stx2 positive) and the other was ETEC (sta positive). Two pathogenic E. coli strains were isolated from crisphead lettuce. EHEC strain was not serotyped by commercial antisera and ETEC was serotyped as O20. EHEC and ETEC strains showed multi-drug resistance against 4 and 7 antibiotics, respectively. These results indicate that retail fresh vegetables seem to be not an important source of human EHEC and ETEC infection in the Mekong Delta, Vietnam.</p>","PeriodicalId":54373,"journal":{"name":"Food Hygiene and Safety Science","volume":"62 3","pages":"94-99"},"PeriodicalIF":0.3,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39147973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
Food Hygiene and Safety Science
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