We experienced a pufferfish poisoning case where no food residue was available to detect a causative agent. However, tetrodotoxin (TTX) was detected in vomit and urine samples from a patient using LC-MS/MS. Furthermore, we found a significant matrix effect in this analysis, indicating that the retention time of vomit and urine was not identical to the TTX standard solution and measured values multiplied by the dilution factors were not constant. Elimination of this matrix effect was attained by dilution of samples based on the retention time of the TTX standard solution, i.e., 10-time dilution of vomit test sample for LC-MS/MS analysis or 100-200-time dilution of urine one. Further research on urine analytical methods revealed that when TTX concentrations were too low to identify its peak on a chromatogram, TTX could be identified through a dilution procedure. It also showed that the application of the matrix-added TTX standard solution was effective for quantitative analysis under the influence of the matrix.
{"title":"[Detection of Tetrodotoxin in Vomit and Urine from a Patient Suffering from Pufferfish Poisoning].","authors":"Junko Namba, Toyohiro Urayama, Hidefumi Kaneko, Atsushi Sato, Kae Fujimoto, Noriko Shigeta","doi":"10.3358/shokueishi.63.225","DOIUrl":"https://doi.org/10.3358/shokueishi.63.225","url":null,"abstract":"<p><p>We experienced a pufferfish poisoning case where no food residue was available to detect a causative agent. However, tetrodotoxin (TTX) was detected in vomit and urine samples from a patient using LC-MS/MS. Furthermore, we found a significant matrix effect in this analysis, indicating that the retention time of vomit and urine was not identical to the TTX standard solution and measured values multiplied by the dilution factors were not constant. Elimination of this matrix effect was attained by dilution of samples based on the retention time of the TTX standard solution, i.e., 10-time dilution of vomit test sample for LC-MS/MS analysis or 100-200-time dilution of urine one. Further research on urine analytical methods revealed that when TTX concentrations were too low to identify its peak on a chromatogram, TTX could be identified through a dilution procedure. It also showed that the application of the matrix-added TTX standard solution was effective for quantitative analysis under the influence of the matrix.</p>","PeriodicalId":54373,"journal":{"name":"Food Hygiene and Safety Science","volume":"63 6","pages":"225-230"},"PeriodicalIF":0.3,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10449907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A novel method was developed for quantification of bisacuron (BC) and dehydrozingerone (DZ), the functional component of turmeric (Curcuma longa.L)-containing foods, using a relative molar sensitivity (RMS) method based on the combination of HPLC-UV and 1H-NMR. The RMSs of BC and DZ using 4-hydroxybenzoic acid ethyl ester (HBE) as the internal standard were calculated to 1.66 and 2.55, respectively. Analysis of fourteen beverage products showed the high correlations between the concentrations of BC and DZ quantified by the RMS method and those quantified by absolute calibration curve method. A collaborative study was conducted by four laboratories on one beverage and one tablet products. The repeatable relative standard deviation (RSDr) of intra-laboratories ranged from 0.7 to 1.7%, and the reproducible relative standard deviation (RSDR) of inter-laboratories ranged from 2.0 to 7.3%. The RMS method enabled the quantification of analytes for which difficultly obtain standard materials such as BC and DZ, using an internal standard for which obtain routinely readily available. This RMS method is expected to be applied to quality control for food products containing turmeric.
{"title":"[Quantitative Analysis of Bisacurone in Turmeric by HPLC Using Relative Molar Sensitivity].","authors":"Kazuo Koyama, Hiroshi Sasako, Yohei Higashi, Hirono Ichikawa, Ayako Nagoya, Takashi Hirao","doi":"10.3358/shokueishi.63.202","DOIUrl":"https://doi.org/10.3358/shokueishi.63.202","url":null,"abstract":"<p><p>A novel method was developed for quantification of bisacuron (BC) and dehydrozingerone (DZ), the functional component of turmeric (Curcuma longa.L)-containing foods, using a relative molar sensitivity (RMS) method based on the combination of HPLC-UV and <sup>1</sup>H-NMR. The RMSs of BC and DZ using 4-hydroxybenzoic acid ethyl ester (HBE) as the internal standard were calculated to 1.66 and 2.55, respectively. Analysis of fourteen beverage products showed the high correlations between the concentrations of BC and DZ quantified by the RMS method and those quantified by absolute calibration curve method. A collaborative study was conducted by four laboratories on one beverage and one tablet products. The repeatable relative standard deviation (RSD<sub>r</sub>) of intra-laboratories ranged from 0.7 to 1.7%, and the reproducible relative standard deviation (RSD<sub>R</sub>) of inter-laboratories ranged from 2.0 to 7.3%. The RMS method enabled the quantification of analytes for which difficultly obtain standard materials such as BC and DZ, using an internal standard for which obtain routinely readily available. This RMS method is expected to be applied to quality control for food products containing turmeric.</p>","PeriodicalId":54373,"journal":{"name":"Food Hygiene and Safety Science","volume":"63 6","pages":"202-209"},"PeriodicalIF":0.3,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10816272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
We investigated the prevalence of Campylobacter jejuni, Campylobacter coli, Salmonella, enterohemorrhagic Escherichia coli, enterotoxigenic Escherichia coli, Yersinia enterocolitica, and Escherichia albertii in domestic chicken and pork sold at retail stores in Saitama Prefecture, Japan. Campylobacter was detected in 35.7% (60/168) of chicken samples and 7.3% (14/190) of pork samples. C. jejuni and C. coli were predominant in chicken and pork, respectively. Salmonella was found in 58.1% (100/172) of chicken samples and 19.9% (41/206) of pork samples. Moreover, Salmonella Schwarzengrund was the major serovar observed in chicken isolates, whereas S. Typhimurium monophasic variant was in pork isolates. Furthermore, ETEC was found in 0.6% (1/160) of chicken samples and 2.4% (5/206) of pork samples. Y. enterocolitica was absent from all (83/83) chicken samples but was present in 9.3% (18/193) of pork samples, with a prevalence in pork tongues as high as 21.0% (13/62 samples). However, EHEC and E. albertii were not detected in our study. Therefore, the results of this study indicate that chicken was highly contaminated with Campylobacter sp. and Salmonella, and pork was with Y. enterocolitica serotype O3, Campylobacter sp., Salmonella, and ETEC.
{"title":"[Prevalence of Foodborne Pathogens in Retail Meat in Saitama, Japan].","authors":"Nozomi Sakakida, Mika Sato, Satomi Kando, Kaori Kashima, Shinichi Shimada, Rie Ishii","doi":"10.3358/shokueishi.63.151","DOIUrl":"https://doi.org/10.3358/shokueishi.63.151","url":null,"abstract":"<p><p>We investigated the prevalence of Campylobacter jejuni, Campylobacter coli, Salmonella, enterohemorrhagic Escherichia coli, enterotoxigenic Escherichia coli, Yersinia enterocolitica, and Escherichia albertii in domestic chicken and pork sold at retail stores in Saitama Prefecture, Japan. Campylobacter was detected in 35.7% (60/168) of chicken samples and 7.3% (14/190) of pork samples. C. jejuni and C. coli were predominant in chicken and pork, respectively. Salmonella was found in 58.1% (100/172) of chicken samples and 19.9% (41/206) of pork samples. Moreover, Salmonella Schwarzengrund was the major serovar observed in chicken isolates, whereas S. Typhimurium monophasic variant was in pork isolates. Furthermore, ETEC was found in 0.6% (1/160) of chicken samples and 2.4% (5/206) of pork samples. Y. enterocolitica was absent from all (83/83) chicken samples but was present in 9.3% (18/193) of pork samples, with a prevalence in pork tongues as high as 21.0% (13/62 samples). However, EHEC and E. albertii were not detected in our study. Therefore, the results of this study indicate that chicken was highly contaminated with Campylobacter sp. and Salmonella, and pork was with Y. enterocolitica serotype O3, Campylobacter sp., Salmonella, and ETEC.</p>","PeriodicalId":54373,"journal":{"name":"Food Hygiene and Safety Science","volume":"63 4","pages":"151-157"},"PeriodicalIF":0.3,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40335167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Arsenic (As) and heavy metals (Cd, Hg, Pb, and Cu) and pesticide residues in 14 edible insects were investigated. The maximum levels of elements were 6.15 for As, 0.82 for Cd, 0.50 for Hg, 0.67 for Pb, and 297.7 ppm for Cu. Fenobucarb (or BPMC) has been quantified through GC- and LC-MS/MS analysis at a concentration of approximately 3 ppm. Further studies of the contaminants may help ensure the safety of edible insect consumption.
{"title":"[Survey of Arsenic/Heavy Metals and Pesticide Residues in Edible Insects for Human Consumption or Supplied in Japan].","authors":"Yugo Kitahara, Hiroshi Nomura, Nanami Nishihara, Takuya Ueda, Satoru Watanabe, Isao Saito, Jun Ueyama","doi":"10.3358/shokueishi.63.136","DOIUrl":"https://doi.org/10.3358/shokueishi.63.136","url":null,"abstract":"<p><p>Arsenic (As) and heavy metals (Cd, Hg, Pb, and Cu) and pesticide residues in 14 edible insects were investigated. The maximum levels of elements were 6.15 for As, 0.82 for Cd, 0.50 for Hg, 0.67 for Pb, and 297.7 ppm for Cu. Fenobucarb (or BPMC) has been quantified through GC- and LC-MS/MS analysis at a concentration of approximately 3 ppm. Further studies of the contaminants may help ensure the safety of edible insect consumption.</p>","PeriodicalId":54373,"journal":{"name":"Food Hygiene and Safety Science","volume":"63 4","pages":"136-140"},"PeriodicalIF":0.3,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40336839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
To evaluate the effects of handling "not detectable" residues (ND: <0.01 mg/kg) in the pulp and detectable residues in the pits on the calculation of pesticide residue in the whole fruit, residue levels in the pulp, peel, and pits of loquat fruits were separately analyzed. Following conventional Japanese agricultural practices, 16 pesticides were sprayed at the maximum application rates in three test fields. All target pesticides were detected at quantifiable levels in the peel (n=144). In contrast, the percentages of detected pesticides in the pulp and pits were 42% (n=61) and 36% (n=52), respectively. Most pesticide residues were present in the peel. For comparison, the pesticide residue levels in the whole fruits were determined based on three indices: the highest estimate (H), calculated using the measured residue levels in the pits and by replacing the ND residues in the pulp as the limit of quantification (LOQ) values; conventional estimate (C), calculated by neglecting all residues in the pits (0 mg/kg) and replacing the ND residues in the pulp as LOQ values; and the lowest estimate (L), calculated by neglecting all residues in the pits and the ND residues in the pulp (0 mg/kg). The L/C and H/C ratios ranged from 74% (L/C) to 106% (H/C). In seven of eighty-three cases with less than 90% difference, residue levels in the whole loquat fruits were low (≤0.06 mg/kg), with the actual range being equal to or below the minimum unit of 0.01. In comparison of three field datasets, the range of residue levels was estimated to be 2.77 mg/kg. Based on the results of separate analysis, handling of ND residues in the pulp and detectable residues in the pits did not significantly affect the calculated pesticide residue levels in the whole loquat fruits.
为了评估处理 "检测不到 "残留物(ND.ND:
{"title":"[Separate Analysis of Loquat Fruit Pulp, Peel, and Pits to Calculate Pesticide Residue Levels in the Whole Commodity].","authors":"Shinobu Hikino, Tomonari Yajima, Michiko Sakasai, Naruto Tomiyama, Kazuaki Iijima, Kazutoshi Ohyama","doi":"10.3358/shokueishi.63.62","DOIUrl":"10.3358/shokueishi.63.62","url":null,"abstract":"<p><p>To evaluate the effects of handling \"not detectable\" residues (ND: <0.01 mg/kg) in the pulp and detectable residues in the pits on the calculation of pesticide residue in the whole fruit, residue levels in the pulp, peel, and pits of loquat fruits were separately analyzed. Following conventional Japanese agricultural practices, 16 pesticides were sprayed at the maximum application rates in three test fields. All target pesticides were detected at quantifiable levels in the peel (n=144). In contrast, the percentages of detected pesticides in the pulp and pits were 42% (n=61) and 36% (n=52), respectively. Most pesticide residues were present in the peel. For comparison, the pesticide residue levels in the whole fruits were determined based on three indices: the highest estimate (H), calculated using the measured residue levels in the pits and by replacing the ND residues in the pulp as the limit of quantification (LOQ) values; conventional estimate (C), calculated by neglecting all residues in the pits (0 mg/kg) and replacing the ND residues in the pulp as LOQ values; and the lowest estimate (L), calculated by neglecting all residues in the pits and the ND residues in the pulp (0 mg/kg). The L/C and H/C ratios ranged from 74% (L/C) to 106% (H/C). In seven of eighty-three cases with less than 90% difference, residue levels in the whole loquat fruits were low (≤0.06 mg/kg), with the actual range being equal to or below the minimum unit of 0.01. In comparison of three field datasets, the range of residue levels was estimated to be 2.77 mg/kg. Based on the results of separate analysis, handling of ND residues in the pulp and detectable residues in the pits did not significantly affect the calculated pesticide residue levels in the whole loquat fruits.</p>","PeriodicalId":54373,"journal":{"name":"Food Hygiene and Safety Science","volume":"43 1","pages":"62-69"},"PeriodicalIF":0.2,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91386882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Since amantadine, rimantadine, arbidol, laninamvir, oseltamivir, peramivir, and zanamivir may be used as antiviral agents to treat avian influenza, we herein developed a simultaneous assay using LC-MS/MS. This method was applied to chicken products (including yakitori (grilled chicken), fried chicken, chicken steak, and boiled eggs) as well as chicken tissues (muscle, fat, the liver, gizzards, and heart) and eggs.Samples were extracted with methanol-water (9 : 1), purified by a tandem column with an InertSep® MAX cartridge (upper part) and InertSep® MCX cartridge (lower part), and then measured by LC-MS/MS. The sample matrix had no effect on the identification of compounds. Chromatographic separation was performed on a ZIC-HILIC column using a mobile phase of 1% acetic acid solution and 1% acetic acid solution in acetonitrile, resulting in complete separation and other obstructive peaks from the sample matrices. An external solvent calibration curve was used for quantification.The application of the method to 6 samples of chicken tissues and eggs achieved good results of between 77.9 and 97.5% for trueness and between 1.7 and 9.2% for concurrent accuracy. The method was also applied to 9 samples of processed products, including grilled chicken and fried chicken, and achieved good results with true percentages ranging between 72.6 and 99.2% and concurrent accuracies between 3.0 and 11.2%. Therefore, the developed method may also be applied to processed products.The limit of quantification (LOQ) of the developed method was 0.01 mg/kg.The method was then applied to 42 types of commercial processed products, including yakitori, fried chicken, steamed chicken, chicken steak, and boiled eggs, and no antiviral agents were detected.Collectively, the present results confirmed that the method developed herein is applicable to not only chicken tissues, but also their processed products.
{"title":"[Simultaneous Analysis of 7 Antiviral Agents in Chicken Tissues and Processed Products by LC-MS/MS].","authors":"Takayuki Asakura, Mariko Kitamura, Miho Yasumoto, Yoshitaka Takeuchi, Mitsuo Nakazato, Kazuo Yasuda","doi":"10.3358/shokueishi.63.1","DOIUrl":"10.3358/shokueishi.63.1","url":null,"abstract":"<p><p>Since amantadine, rimantadine, arbidol, laninamvir, oseltamivir, peramivir, and zanamivir may be used as antiviral agents to treat avian influenza, we herein developed a simultaneous assay using LC-MS/MS. This method was applied to chicken products (including yakitori (grilled chicken), fried chicken, chicken steak, and boiled eggs) as well as chicken tissues (muscle, fat, the liver, gizzards, and heart) and eggs.Samples were extracted with methanol-water (9 : 1), purified by a tandem column with an InertSep<sup>®</sup> MAX cartridge (upper part) and InertSep<sup>®</sup> MCX cartridge (lower part), and then measured by LC-MS/MS. The sample matrix had no effect on the identification of compounds. Chromatographic separation was performed on a ZIC-HILIC column using a mobile phase of 1% acetic acid solution and 1% acetic acid solution in acetonitrile, resulting in complete separation and other obstructive peaks from the sample matrices. An external solvent calibration curve was used for quantification.The application of the method to 6 samples of chicken tissues and eggs achieved good results of between 77.9 and 97.5% for trueness and between 1.7 and 9.2% for concurrent accuracy. The method was also applied to 9 samples of processed products, including grilled chicken and fried chicken, and achieved good results with true percentages ranging between 72.6 and 99.2% and concurrent accuracies between 3.0 and 11.2%. Therefore, the developed method may also be applied to processed products.The limit of quantification (LOQ) of the developed method was 0.01 mg/kg.The method was then applied to 42 types of commercial processed products, including yakitori, fried chicken, steamed chicken, chicken steak, and boiled eggs, and no antiviral agents were detected.Collectively, the present results confirmed that the method developed herein is applicable to not only chicken tissues, but also their processed products.</p>","PeriodicalId":54373,"journal":{"name":"Food Hygiene and Safety Science","volume":"1 1","pages":"1-11"},"PeriodicalIF":0.2,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77084603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In the pharmaceutical ingredients contamination testing of 702 commercial dietary supplement products, during fiscal years 2014-2021, 14 pharmaceutical ingredients, barrenwort, leaf axils of senna, and small leaf of senna were detected in 28 products. Screening and confirmation of the pharmaceutical ingredients in the products were performed by ultra-high performance liquid chromatography with photodiode array detector and ultra-high performance liquid chromatography-quadrupole-kingdon trap mass spectrometry, respectively. In particular, leaf axils and small leaf of senna were identified by stereomicroscopy and scanning electron microscopy. Furthermore, we found several pharmaceutical ingredients that exceeded the daily medicated dosage; therefore, it is important to prevent the distribution of such products to prevent the occurrence of health hazard. For that reason, it is necessary to continue the sample purchasing and testing systems to monitor the distribution of products containing pharmaceutical ingredients.
{"title":"[Pharmaceutical Ingredients Detected in Dietary Supplements Purchased by Chiba Prefecture (Fiscal Years 2014-2021)].","authors":"Tomohide Fukiwake, Keigo Enomoto, Hiroki Yoshino, Katsuya Uchimoto, Maki Nishimura, Hiroshi Akiyama","doi":"10.3358/shokueishi.63.141","DOIUrl":"https://doi.org/10.3358/shokueishi.63.141","url":null,"abstract":"<p><p>In the pharmaceutical ingredients contamination testing of 702 commercial dietary supplement products, during fiscal years 2014-2021, 14 pharmaceutical ingredients, barrenwort, leaf axils of senna, and small leaf of senna were detected in 28 products. Screening and confirmation of the pharmaceutical ingredients in the products were performed by ultra-high performance liquid chromatography with photodiode array detector and ultra-high performance liquid chromatography-quadrupole-kingdon trap mass spectrometry, respectively. In particular, leaf axils and small leaf of senna were identified by stereomicroscopy and scanning electron microscopy. Furthermore, we found several pharmaceutical ingredients that exceeded the daily medicated dosage; therefore, it is important to prevent the distribution of such products to prevent the occurrence of health hazard. For that reason, it is necessary to continue the sample purchasing and testing systems to monitor the distribution of products containing pharmaceutical ingredients.</p>","PeriodicalId":54373,"journal":{"name":"Food Hygiene and Safety Science","volume":"63 4","pages":"141-150"},"PeriodicalIF":0.3,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40336840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.3358/shokueishi.63.105
Kyoko Takagi, Hitoshi Miyazaki
A rapid determination method for emergency response to health crisis caused by metals in foods, was developed using microwave decomposition equipment and inductively coupled plasma atomic emission spectroscopy (ICP-AES). The method was assessed for 18 elements (Al, As, B, Cd, Co, Cr, Cu, Fe, Hg, Mn, Mo, Ni, Pb, Sb, Se, Sn, Tl and Zn) in 5 kinds of beverages and 7 kinds of foods. A single-laboratory method validation study was performed using food samples added with 20 mg/kg of each metal. Trueness was 88-108% and intralaboratory reproducibility was 0.2-11.3%. Time required for analysis was less than 3 hr. Thus, the presented method could be useful for rapid analysis of metals involved food poisoning cases.
{"title":"[A Rapid Determination Method of Metals in Foods for Emergency Response to Health Crisis].","authors":"Kyoko Takagi, Hitoshi Miyazaki","doi":"10.3358/shokueishi.63.105","DOIUrl":"https://doi.org/10.3358/shokueishi.63.105","url":null,"abstract":"<p><p>A rapid determination method for emergency response to health crisis caused by metals in foods, was developed using microwave decomposition equipment and inductively coupled plasma atomic emission spectroscopy (ICP-AES). The method was assessed for 18 elements (Al, As, B, Cd, Co, Cr, Cu, Fe, Hg, Mn, Mo, Ni, Pb, Sb, Se, Sn, Tl and Zn) in 5 kinds of beverages and 7 kinds of foods. A single-laboratory method validation study was performed using food samples added with 20 mg/kg of each metal. Trueness was 88-108% and intralaboratory reproducibility was 0.2-11.3%. Time required for analysis was less than 3 hr. Thus, the presented method could be useful for rapid analysis of metals involved food poisoning cases.</p>","PeriodicalId":54373,"journal":{"name":"Food Hygiene and Safety Science","volume":"63 3","pages":"105-108"},"PeriodicalIF":0.3,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40523906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.3358/shokueishi.62.20
Chiharu Nishijima, Kaoru Sato, Tsuyoshi Chiba
The role of dietitians is important for consumers to practice self-care, which includes the use of the "Foods with Function Claims (FFC)". In this study, a nationwide internet survey was conducted to clarify the understanding and attitudes of dietitians towards the FFC 1 year and 4 years after its introduction (Surveys were conducted in 2015 and 2019, respectively). In the survey of 1 year after the introduction, the ratio of recognition of the FFC was 98%; however, only 35% correctly understood the characteristics of the FFC. Similarly, in the survey of 4 year after the introduction, only 42% of dietitians correctly answered the characteristics. At 4 year after, 56% of dietitians currently or previously have used the FFC. The dietitians who had been consulted about the use of the FFC accounted for 22% and about the adverse events accounted for 15%. The advice they gave at the consultation differed according to their experience of the use of the FFC. Of the ones who never used the FFC, 25% referred no information sources for the FFC. The place to educate primarily working dietitians needs to be prepared to let them provide appropriate information to consumers to support self-care including the appropriate use of the FFC.
{"title":"[Understanding and Attitudes of Dietitians towards \"Foods with Function Claims\"].","authors":"Chiharu Nishijima, Kaoru Sato, Tsuyoshi Chiba","doi":"10.3358/shokueishi.62.20","DOIUrl":"https://doi.org/10.3358/shokueishi.62.20","url":null,"abstract":"The role of dietitians is important for consumers to practice self-care, which includes the use of the \"Foods with Function Claims (FFC)\". In this study, a nationwide internet survey was conducted to clarify the understanding and attitudes of dietitians towards the FFC 1 year and 4 years after its introduction (Surveys were conducted in 2015 and 2019, respectively). In the survey of 1 year after the introduction, the ratio of recognition of the FFC was 98%; however, only 35% correctly understood the characteristics of the FFC. Similarly, in the survey of 4 year after the introduction, only 42% of dietitians correctly answered the characteristics. At 4 year after, 56% of dietitians currently or previously have used the FFC. The dietitians who had been consulted about the use of the FFC accounted for 22% and about the adverse events accounted for 15%. The advice they gave at the consultation differed according to their experience of the use of the FFC. Of the ones who never used the FFC, 25% referred no information sources for the FFC. The place to educate primarily working dietitians needs to be prepared to let them provide appropriate information to consumers to support self-care including the appropriate use of the FFC.","PeriodicalId":54373,"journal":{"name":"Food Hygiene and Safety Science","volume":"62 1","pages":"20-27"},"PeriodicalIF":0.3,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25433820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
From July 2017 to January 2019, total of 645 retail fresh vegetables collected from 19 retail shops and markets was investigated to know the contamination of enterohemorrhagic Escherichia coli (EHEC) and enterotoxigenic E. coli (ETEC). Of 645 samples, 2 samples (0.3%) were positive for pathogenic E. coli. Of 2 pathogenic E. coli positive samples, 1 was EHEC (stx2 positive) and the other was ETEC (sta positive). Two pathogenic E. coli strains were isolated from crisphead lettuce. EHEC strain was not serotyped by commercial antisera and ETEC was serotyped as O20. EHEC and ETEC strains showed multi-drug resistance against 4 and 7 antibiotics, respectively. These results indicate that retail fresh vegetables seem to be not an important source of human EHEC and ETEC infection in the Mekong Delta, Vietnam.
{"title":"[Contamination of Pathogenic Escherichia coli in Retail Fresh Vegetables in the Mekong Delta, Vietnam].","authors":"Shunsuke Ikeuchi, Bui Thi Hien, Nguyen Khanh Thuan, Ly Thi, Yukiko Hara-Kudo, Takahide Taniguchi, Hideki Hayashidani","doi":"10.3358/shokueishi.62.94","DOIUrl":"https://doi.org/10.3358/shokueishi.62.94","url":null,"abstract":"<p><p>From July 2017 to January 2019, total of 645 retail fresh vegetables collected from 19 retail shops and markets was investigated to know the contamination of enterohemorrhagic Escherichia coli (EHEC) and enterotoxigenic E. coli (ETEC). Of 645 samples, 2 samples (0.3%) were positive for pathogenic E. coli. Of 2 pathogenic E. coli positive samples, 1 was EHEC (stx2 positive) and the other was ETEC (sta positive). Two pathogenic E. coli strains were isolated from crisphead lettuce. EHEC strain was not serotyped by commercial antisera and ETEC was serotyped as O20. EHEC and ETEC strains showed multi-drug resistance against 4 and 7 antibiotics, respectively. These results indicate that retail fresh vegetables seem to be not an important source of human EHEC and ETEC infection in the Mekong Delta, Vietnam.</p>","PeriodicalId":54373,"journal":{"name":"Food Hygiene and Safety Science","volume":"62 3","pages":"94-99"},"PeriodicalIF":0.3,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39147973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}