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Kaempferol Enhances Sperm Post-Thaw Survival by Its Cryoprotective and Antioxidant Behavior 山奈酚通过其冷冻保护和抗氧化行为提高精子解冻后的存活率
3区 工程技术 Q2 MECHANICS Pub Date : 2023-09-28 DOI: 10.3390/stresses3040047
Štefan Baňas, Filip Benko, Michal Ďuračka, Norbert Lukáč, Eva Tvrdá
This study examined the effects of three selected doses of kaempferol (KAE; 12.5, 25 or 50 μM) on bovine sperm motility and oxidative profile directly related to cold storage. We also elucidated the effect of KAE on the expression profiles of heat shock proteins (HSPs) 70 and 90 as well as the pro-apoptotic BCL2-associated X (BAX) protein and the anti-apoptotic B-cell lymphoma 2 (Bcl-2) protein. Frozen samples supplemented with KAE were compared with a native control (fresh spermatozoa) and a cryopreserved control, frozen in the absence of KAE. Our results demonstrate that the administration of all KAE doses led to a higher degree of sperm motility (p < 0.05) when compared with the cryopreserved control. The highest levels of protection of sperm DNA (p < 0.05), lipids (p < 0.05) and proteins (p < 0.05) were detected in samples exposed to 25 μM KAE when compared with samples frozen without KAE. Administration of 25 μM KAE led to a significant increase in HSP70 and HSP90 (p < 0.05) when compared with the unsupplemented frozen control. No significant differences were observed in the expression patterns of BAX; however, a significant up-regulation of Bcl-2 protein was observed in the frozen samples enriched with 25 μM KAE when compared with the cryopreserved control (p < 0.05). In summary, we may consider KAE as an effective agent in stabilizing the sperm membranes by preventing reactive oxygen species (ROS) overproduction in the mitochondria and subsequent oxidative damage to molecules critical for a proper sperm architecture and function. These protective properties of KAE may lead to higher post-thaw sperm activity and viability.
本研究考察了三种选定剂量山奈酚(KAE;12.5、25或50 μM)对牛精子活力和氧化谱的影响。我们还阐明了KAE对热休克蛋白(HSPs) 70和90以及促凋亡bcl2相关的X (BAX)蛋白和抗凋亡b细胞淋巴瘤2 (Bcl-2)蛋白表达谱的影响。将添加KAE的冷冻样本与天然对照(新鲜精子)和冷冻保存的对照组(在没有KAE的情况下冷冻)进行比较。我们的研究结果表明,所有KAE剂量的管理导致更高程度的精子活力(p <0.05),与冷冻保存对照比较。对精子DNA的最高保护水平(p <0.05),脂质(p <0.05)和蛋白质(p <与未冷冻的样品相比,暴露于25 μM KAE的样品检测到0.05)。给药25 μM KAE导致HSP70和HSP90显著升高(p <0.05),与未添加冷冻对照比较。BAX表达谱差异无统计学意义;然而,与冷冻保存的对照组相比,25 μM KAE富集的冷冻样品中Bcl-2蛋白显著上调(p <0.05)。综上所述,我们可以认为KAE是一种有效的稳定精子膜的药物,它可以防止线粒体中活性氧(ROS)的过量产生,以及随后对精子结构和功能至关重要的分子的氧化损伤。KAE的这些保护特性可能导致更高的解冻后精子活性和生存能力。
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引用次数: 1
Abscisic Acid: Metabolism, Signaling, and Crosstalk with Other Phytohormones under Heavy Metal Stress 重金属胁迫下脱落酸的代谢、信号传导及与其他植物激素的串扰
3区 工程技术 Q2 MECHANICS Pub Date : 2023-09-22 DOI: 10.3390/stresses3040046
Ambreen Bano, Kratika Singh, Surendra Pratap Singh, Pooja Sharma
Heavy metal (HM) stress poses a global risk to crops, ecological systems, and human health. It disrupts cellular ionic equilibrium, cell membrane integrity, metabolic balance, and the activities of enzymes and proteins, severely impacting physiological processes, plant development, and agricultural productivity. Although plants naturally activate defense mechanisms to mitigate the adverse effects of HM stress, they cannot completely prevent them. Phytohormones counter HM toxicity, aiding growth. External application and internal regulation via signaling/biosynthesis genes offer defense against HM-induced damage. A pivotal signaling molecule in plant adaptive responses to environmental stressors, including HM toxicity, is abscisic acid (ABA). Despite ABA’s role in abiotic stress responses such as drought and salinity, its function and crosstalk with other phytohormones under HM stress remain poorly understood. Nonetheless, exogenously applied ABA serves as a strategic approach to enhancing plants’ resistance to HM toxicity by promoting osmolyte accumulation and reinforcing antioxidant activity. ABA significantly regulates various plant growth and metabolic activities under diverse environmental conditions. This review highlights the effects of HM stress on plants and explores ABA involvement in production, signaling, catabolism, and transport within plant tissues. The purpose of this paper is to shed light on the complex interplay between the metabolism of ABA, its signaling, and its interactions with other phytohormones (e.g., auxins, gibberellins, and ethylene) during HM exposure. Furthermore, we delve into the function of ABA to mitigate HM stress and elucidate its interactions with other phytohormones.
重金属胁迫对作物、生态系统和人类健康构成全球性风险。它破坏细胞离子平衡、细胞膜完整性、代谢平衡以及酶和蛋白质的活性,严重影响生理过程、植物发育和农业生产力。虽然植物自然激活防御机制来减轻HM胁迫的不利影响,但它们不能完全阻止它们。植物激素对抗HM毒性,帮助生长。外部应用和通过信号/生物合成基因的内部调节可以防御hm诱导的损伤。在植物对环境胁迫(包括HM毒性)的适应性反应中,一个关键的信号分子是脱落酸(ABA)。尽管ABA在干旱和盐胁迫等非生物胁迫反应中发挥作用,但其在HM胁迫下的功能及其与其他植物激素的相互作用尚不清楚。尽管如此,外源应用ABA可以通过促进渗透物积累和增强抗氧化活性来增强植物对HM毒性的抗性。ABA对不同环境条件下植物的生长和代谢活性具有显著的调节作用。本文重点介绍了HM胁迫对植物的影响,并探讨了ABA在植物组织内的产生、信号传导、分解代谢和运输中的作用。本文的目的是阐明在HM暴露过程中ABA的代谢、信号传导及其与其他植物激素(如生长素、赤霉素和乙烯)的相互作用之间的复杂相互作用。此外,我们还深入研究了ABA减轻HM胁迫的功能,并阐明了其与其他植物激素的相互作用。
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引用次数: 0
Preserving the Authenticity of ST25 Rice (Oryza sativa) from the Mekong Delta: A Multivariate Geographical Characterization Approach 湄公河三角洲ST25稻(Oryza sativa)的真实性保护:多元地理表征方法
3区 工程技术 Q2 MECHANICS Pub Date : 2023-09-18 DOI: 10.3390/stresses3030045
Dinh Tri Bui, Ngoc Minh Truong, Viet Anh Le, Hoang Khanh Nguyen, Quang Minh Bui, Van Thinh Pham, Quang Trung Nguyen
The research centers around ST25, a recently acclaimed rice variety lauded as Vietnam’s premier offering. However, its ability to substantiate its origin is impeded by the rampant proliferation of counterfeit derivatives within the market. A distinctive methodology is posited herein, intertwining the attributes of Fourier Transform Infrared Spectroscopy (FTIR) and Inductively Coupled Plasma Mass Spectrometry (ICP-MS) analyses, augmented using Principal Component Analysis (PCA). The primary objective is to meticulously ascertain the unadulterated geographic provenance of the ST25 rice cultivar. The findings unequivocally underscore the emergence of a conspicuous taxonomy within the ST25 rice samples sourced from Soc Trang, underpinned by the utilization of both FTIR and ICP-MS datasets. Remarkably, the discernment of eight elemental constituents (27Al, 59Co, 44Ca, 57Fe, 60Ni, 63Cu, 93Nb, and 98Mo) has been adjudicated as pivotal in ascribing geospatial classification. The ramifications of this proposed modality encompass not only the authentication of the subject rice variety but also extend to the validation of similar grain types. Functioning as a potent deterrent against the omnipresent specter of food counterfeiting within the market milieu, this methodology occupies a pivotal niche.
该研究以ST25为中心,这是一种最近备受赞誉的大米品种,被誉为越南的首选品种。然而,其证实其来源的能力受到市场上假冒衍生品猖獗扩散的阻碍。本文提出了一种独特的方法,将傅里叶变换红外光谱(FTIR)和电感耦合等离子体质谱(ICP-MS)分析的属性交织在一起,并使用主成分分析(PCA)进行增强。主要目的是精心确定ST25水稻品种的纯正地理来源。这些发现明确强调了在利用FTIR和ICP-MS数据集的基础上,在来自Soc Trang的ST25水稻样本中出现了一个明显的分类。值得注意的是,8种元素成分(27Al, 59Co, 44Ca, 57Fe, 60Ni, 63Cu, 93Nb和98Mo)的识别被认为是地理空间分类的关键。这种提出的模式的后果不仅包括对主题水稻品种的认证,而且还扩展到对类似谷物类型的验证。作为对市场环境中无所不在的食品假冒幽灵的有力威慑,这种方法占据了关键的利基。
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引用次数: 0
Peridynamic simulation of heat transfer during quenching of semi-solid plate with occurrence of hot cracks 存在热裂纹的半固态板淬火过程传热的动力学模拟
3区 工程技术 Q2 MECHANICS Pub Date : 2023-09-14 DOI: 10.1080/01495739.2023.2253856
Jijo Prasad Jayaprasad Remani, Saravanakumar Palanisamy, Ashok Kumar Nallathambi, Selda Oterkus, Daniel Juhre, Eckehard Specht
AbstractQuenching plays an important role in the material processing. Water impingement jet quenching involves phenomena such as nucleate boiling, Leidenfrost effect, rewetting, etc. Stronger thermal gradients could induce cracks in the material. Thermal boundary conditions of the process depends on temperature, space, time, and material discontinuity. This study mainly focuses on the thermal modeling of moving water jet quenching of semi-solid hot vertical aluminum plate with latent heat release during the solidification by using Peridynamics (PD). The obtained thermal profile is compared with the infrared camera’s thermal field data to identify the boiling curve parameters on the cracked plate. The PD model with the incorporation of cracking dynamics is giving agreeable results with the experimental thermal field. Modeling quenching heat transfer using PD will enhance the coupling of hot tear origination and propagation simulation, which is quite challenging with other numerical methods. The application of PD in quenching heat transfer with phase change is the first of its kind. The advantages and limitations of the peridynamic approach for this problem is critically evaluated and the simulated results are compared with the experimental measurement of temperature profiles.Keywords: Hot cracksperidynamicsphase changequench heat transferquenchingsemi-solid state AcknowledgmentsThe authors express their gratitude to Dr. Nyein Nyein Linn (AIRTF scholar) who came from Myanmar for the short-term research training at NIT-T. We express our sincere thanks to the project students Manu Aatitya R P, Yogeesh S, and Sriranga V who helped us to develop the PD code in MATLAB.Authors’ contributionsJijo Prasad Jayaprasad Remani: Writing – Original Draft, Methodology, Investigation, Formal analysis, Visualization, Software, Validation.Saravanakumar Palanisamy: Experimental measurement and observations, Software, Validation.Ashok Kumar Nallathambi: Supervision, Conceptualization, Writing – Review & Editing, Project administration, Software.Selda Oterkus: Peridynamic formulation of heat transfer – Methodology.Daniel Juhre, and Eckehard Specht: Experimental measurement and observations – Resources, Investigation.Data availability statementThe raw/processed data required to reproduce these findings cannot be shared at this time as the data also forms part of an ongoing study.Disclosure statementThe authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.Additional informationFundingThis work is financially supported by the Science and Engineering Research Board (SERB-DST), India under the project SRG/2019/001463. OvGU Magdeburg, supported for the scholar’s experimentation under funding GRK 1554.
摘要淬火在材料加工中起着重要的作用。水冲击射流淬火涉及到成核沸腾、莱顿弗罗斯特效应、再润湿等现象。较强的热梯度会导致材料出现裂纹。过程的热边界条件取决于温度、空间、时间和材料的不连续性。本文主要利用周动力学(periddynamics, PD)方法对含潜热释放的半固态热立铝板的流动水射流淬火过程进行了热模拟。将所得热剖面与红外热场数据进行对比,确定裂纹板上的沸腾曲线参数。结合裂纹动力学的PD模型与实验热场的关系得到了满意的结果。利用PD方法模拟淬火传热可以增强热撕裂产生和传播模拟的耦合性,这是其他数值方法所无法比拟的。PD在相变淬火传热中的应用尚属首次。对该问题的周动力学方法的优点和局限性进行了批判性评价,并将模拟结果与温度分布的实验测量结果进行了比较。关键词:热裂纹,动力学,相变,传热,淬火,半固态感谢来自缅甸的Nyein Nyein Linn博士(AIRTF学者)来nitt进行短期研究培训。我们衷心感谢项目学生Manu Aatitya R P, Yogeesh S和Sriranga V,他们帮助我们在MATLAB中开发了PD代码。jijo Prasad Jayaprasad Remani:写作——原稿、方法论、调查、形式分析、可视化、软件、验证。Saravanakumar Palanisamy:实验测量和观察,软件,验证。Ashok Kumar Nallathambi:监督,概念化,写作-审查和编辑,项目管理,软件。Selda Oterkus:热传递的动态公式-方法论。Daniel Juhre和Eckehard Specht:实验测量和观察-资源,调查。数据可用性声明再现这些发现所需的原始/处理数据目前不能共享,因为这些数据也是正在进行的研究的一部分。披露声明作者声明,他们没有已知的竞争经济利益或个人关系,可能会影响本文所报道的工作。本研究由印度科学与工程研究委员会(SERB-DST)资助,项目编号为SRG/2019/001463。马格德堡OvGU,在GRK 1554资助下支持该学者的实验。
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引用次数: 0
Dynamic thermomechanical analysis on stiffened composite plates with damage 带损伤的加筋复合材料板动态热力学分析
3区 工程技术 Q2 MECHANICS Pub Date : 2023-09-14 DOI: 10.1080/01495739.2023.2253877
D. H. Li, S. Ma
AbstractThe dynamic thermomechanical analysis on stiffened composite plates with damages are investigated in this article based on the extended layerwise method (XLWM) and three-dimensional finite elements method; a thermomechanical extended-layerwise/Solid-element method (TELW/SE) is established. In TELW/SE, the thermomechanical extended-layerwise method (TELW) and thermomechanical three-dimensional solid elements are used to simulate plate and stiffeners, respectively. The final governing equations are assembled by using the interface conditions to ensure the compatibility of displacements and temperature, together with the internal force equilibrium. Several typical damages in stiffened composite plates can be described accurately in the proposed method, such as delamination, transverse crack and stiffener/plate interface debonding.Keywords: Delaminationdynamic thermomechanical analysisextended layerwise methodinterface debondingstiffened composite platestransverse crack Disclosure statementNo potential conflict of interest was reported by the author(s).Additional informationFundingThis work was supported by the Natural Science Foundations of China (12072364, U1933102) and Fundamental Research Funds for the Central Universities (3122019074).
摘要基于扩展分层法和三维有限元法,对带损伤的加筋复合材料板进行了动态热力学分析;建立了热力学扩展层/固元法(TELW/SE)。在TELW/SE中,分别采用热机械扩展层法(TELW)和热机械三维实体单元对板和加强筋进行模拟。利用界面条件组合最终控制方程,保证了位移和温度的相容性,以及内力平衡。该方法可以准确描述加筋复合材料板的分层、横向裂纹和加筋板界面脱粘等几种典型损伤。关键词:分层动力学热力学分析扩展分层法界面脱粘加筋复合材料板横向裂纹披露声明作者未报告潜在的利益冲突。基金资助:国家自然科学基金(12072364,U1933102)和中央高校基本科研业务费专项资金(3122019074)。
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引用次数: 0
Analytical solution of the temperature-dependent thermoelastic problem induced by Joule heating and the presence of an elliptic cavity 焦耳加热和椭圆腔引起的温度相关热弹性问题的解析解
3区 工程技术 Q2 MECHANICS Pub Date : 2023-09-14 DOI: 10.1080/01495739.2023.2256823
Kunkun Xie, Haopeng Song, Peter Schiavone, Cunfa Gao
AbstractJoule heat induced by electric current may well lead to thermal stress and contribute to structural failure and crack evolution in a range of modern materials. In this paper, we use complex variable methods to obtain the thermoelastic fields around an elliptic cavity in a plate subjected to remote electric and thermal loads. Of particular interest is the fact that we consider the temperature dependency of the corresponding thermoelastic coefficients. We introduce an analytical method which is verified by the fact that it leads to results which reduce to the classical analytical results in the literature when the thermoelastic coefficients are taken to be temperature-independent. We find that when the elliptic cavity is reduced to the representation of a linear crack, the corresponding stress intensity factor increases to a greater extent under the temperature-dependent assumption versus the corresponding classical results. Our findings are significant in forming the basis for the analysis of the evolution of crack growth under severe temperature gradients such as those occurring as a result of Joule heat loading.Keywords: Crack arrestelectric currentJoule heatstress intensity factorstemperature-dependentthermoelastic fields Disclosure statementNo potential conflict of interest was reported by the author(s).Additional informationFundingThe authors would like to thank support from the National Natural Science Foundation of China (NSFC) (11872203) and China Scholarship Council (CSC). This work is also supported by the Joint Fund of Advanced Aerospace Manufacturing Technology Research (U1937601) and the National Natural Science Foundation of China for Creative Research Groups (No. 51921003). Schiavone thanks the Natural Sciences and Engineering Research Council of Canada (Grant No: RGPIN – 2017 - 03716115112).
摘要电流引起的焦耳热很容易引起热应力,从而导致现代材料的结构破坏和裂纹演化。本文采用复变方法计算了在远端电载荷和热载荷作用下平板椭圆腔周围的热弹性场。特别有趣的是,我们考虑了相应热弹性系数的温度依赖性。本文介绍了一种分析方法,当热弹性系数与温度无关时,其结果与文献中的经典分析结果一致。我们发现,当椭圆空腔被简化为线性裂纹的表示时,在温度相关假设下,相应的应力强度因子比相应的经典结果增加得更大。我们的发现对于分析裂纹在焦耳热载荷等严重温度梯度下的扩展演化具有重要意义。关键词:裂纹阻滞电流焦耳热应力强度因子温度相关热弹性场披露声明作者未报告潜在的利益冲突。作者感谢国家自然科学基金委员会(NSFC)(11872203)和中国国家留学基金委(CSC)的支持。航空航天先进制造技术研究联合基金(U1937601)和国家自然科学基金(51921003)资助。Schiavone感谢加拿大自然科学与工程研究委员会(批准号:RGPIN - 2017 - 03716115112)。
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引用次数: 0
Integrated modeling of microstructure-property-distortion relationship in friction stir additive manufacturing 搅拌摩擦增材制造中组织-性能-变形关系的集成建模
3区 工程技术 Q2 MECHANICS Pub Date : 2023-09-12 DOI: 10.1080/01495739.2023.2256804
Zhao Zhang, Yifei Wang, Zhijun Tan, Daxin Ren
Abstract A Monte Carlo model is established to simulate grain morphologies and combined with a precipitate evolution model to predict the mechanical properties of different zones in different layers of friction stir additive manufacturing (FSAM). Subsequently, a subregion model is established by considering the different mechanical properties at different locations to predict the residual states in FSAM. Results indicate that equiaxed grains are formed in the stirring zone and that the average grain size can be decreased by increasing the build height. The yield strengths in the stirring and heat-affected zones increase along the build direction and decrease with increasing temperature. The error between the predicted and experimental yield strengths is 3.3%. By considering changes in the mechanical properties in the stirring and heat-affected zones, the distortion error between the experimental test and numerical model can be reduced by 25% compared with that between the experimental test and the conventional model. The predicted residual stresses can be reduced by considering the strength reduction caused by the friction stirring effect. As the number of built layers increases, the maximum distortion in FSAM increases.
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引用次数: 1
Numerical simulation of non–linear skin model with energy dissipation during hyperthermia and its validation with experimental data 热疗过程中考虑能量耗散的非线性皮肤模型的数值模拟及实验数据验证
3区 工程技术 Q2 MECHANICS Pub Date : 2023-09-12 DOI: 10.1080/01495739.2023.2253864
Rajneesh Kumar Chaudhary, Jitendra Singh
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引用次数: 0
An analytical solution for multiple inclusions subject to a general applied thermal field 一般应用热场作用下多重内含物的解析解
3区 工程技术 Q2 MECHANICS Pub Date : 2023-09-11 DOI: 10.1080/01495739.2023.2247037
Mahdieh Tajabadi Ebrahimi, Daniel Stephen Balint, Daniele Dini
In this study, we present a general numerical solution to the thermoelastic problem of interacting elliptical inhomogeneities in an infinite matrix material. Our solution provides a framework to study microstructural responses to the external mechanical, thermal, electric, and magnetic fields simultaneously. The proposed semi-analytical method, which enables us to reduce the computational cost and calculate the interactions between many inclusions, is based on conformal mapping, series expansion of the corresponding complex potentials and boundary conditions. The temperature, stress, strain, and displacement functions are derived explicitly in the matrix material and inclusions. The thermal loadings tested in this study include temperature variations and a uniform heat flow. The model is validated against multiple analytical calculations and benchmark numerical problems. The method is ready to apply for simulations with many inclusions.
在本研究中,我们提出了无限矩阵材料中相互作用椭圆非均匀性热弹性问题的一般数值解。我们的解决方案提供了一个框架,可以同时研究外部机械、热、电和磁场对微观结构的响应。所提出的半解析方法基于保角映射、相应复势的级数展开和边界条件,减少了计算成本并计算了许多内含物之间的相互作用。温度、应力、应变和位移函数在基体材料和夹杂物中得到了明确的推导。本研究测试的热负荷包括温度变化和均匀热流。通过多次解析计算和基准数值问题对模型进行了验证。该方法已准备好应用于具有许多内含物的模拟。
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引用次数: 0
A variational inequality approach for the numerical simulation of steel solidification of two-phase continuous casting process 用变分不等式方法模拟两相连铸过程中钢的凝固过程
IF 2.8 3区 工程技术 Q2 MECHANICS Pub Date : 2023-09-08 DOI: 10.1080/01495739.2023.2231988
G. Khenniche, Hocine Sissaoui, Lamine Bouzettouta, Salah Bouhouche
Abstract In this paper, we study a nonlinear parabolic type model with an inequality constraint on the solution describing the phase liquid-solid heat transfer during the solidification process in continuous casting. We consider a semi-discretization with respect to time (implicit Euler method in time) of the studied thermal problem. We have a sequence of stationary constrained problems. Reformulating the problem into a variational inequality problem and thanks to the approximation (linearization) of the nonlinear boundary condition (radiation condition) and appropriate assumptions, we establish a result of existence and uniqueness of the solution of the stationary problem. We also consider a multivalued formulation of the same problem allowing the analysis of the behavior of the iterative relaxation algorithm used for the numerical solution of the discretized problem. Finally, numerical simulations are displayed.
摘要本文研究了一种具有不等式约束的非线性抛物型模型,该模型描述了连铸凝固过程中相液固传热。我们考虑所研究的热问题的半离散化(时间上的隐式欧拉法)。我们有一系列平稳约束问题。将该问题转化为变分不等式问题,并利用非线性边界条件(辐射条件)的近似(线性化)和适当的假设,建立了平稳问题解的存在唯一性结果。我们还考虑了同一问题的多值公式,允许分析用于离散问题数值解的迭代松弛算法的行为。最后进行了数值模拟。
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引用次数: 0
期刊
Journal of Thermal Stresses
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