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Response mechanisms of hepatopancreatic metabolism and gut microbiota in Chinese mitten crab (Eriocheir sinensis) under chronic high-pH stress 中华绒螯蟹肝胰代谢和肠道菌群对慢性高ph胁迫的响应机制
IF 2.2 2区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2026-01-20 DOI: 10.1016/j.cbd.2026.101756
Yufei Liu , Zonglin Yang , Min Zhang , Xiaowu Chen , Xiaodong Jiang
The Chinese mitten crab (Eriocheir sinensis) is a commercially important aquaculture species in China, with its survival highly vulnerable to abnormal water pH. This study subjected E. sinensis to chronic high-pH stress (control: pH = 8; experimental groups: pH = 9 and pH = 10) and integrated hepatopancreas transcriptomic/metabolomic analyses with intestinal microbiota 16S rRNA sequencing to explore its adaptive mechanisms. The results revealed distinct adaptive strategies across stress phases: under short-term (15 days) high-pH stress, E. sinensis maintained somatic homeostasis mainly via rapid regulation of carbohydrate, lipid, protein metabolism, and energy allocation. In contrast, long-term (30 days) stress drove a strategic shift toward accelerated lipid catabolism, enhanced energy metabolism, and activated immune-related pathways. Additionally, high-pH stress significantly altered the intestinal microbiota community structure, marked by increased abundances of Proteobacteria and Pseudomonas, indicating a potential risk of microbial dysbiosis. Collectively, these findings elucidate the physiological adaptation mechanisms of E. sinensis to high-pH environments and lay a theoretical foundation for improving the sustainability of its aquaculture under extreme pH conditions.
中华绒螯蟹(Eriocheir sinensis)是中国重要的商业养殖品种,其生存极易受到异常水体pH的影响。本研究将中华绒螯蟹置于慢性高pH胁迫下(对照组:pH = 8,试验组:pH = 9和pH = 10),结合肝胰脏转录组学/代谢组学分析和肠道微生物群16S rRNA测序,探讨其适应机制。结果揭示了不同胁迫阶段的不同适应策略:在短期(15 d)高ph胁迫下,中华棘豆主要通过快速调节碳水化合物、脂质、蛋白质代谢和能量分配来维持体细胞稳态。相比之下,长期(30天)应激推动了脂质分解代谢加速、能量代谢增强和免疫相关途径激活的战略转变。此外,高ph胁迫显著改变了肠道微生物群落结构,其特征是变形杆菌和假单胞菌的丰度增加,表明存在微生物生态失调的潜在风险。综上所述,这些发现阐明了中华鲟对高pH环境的生理适应机制,为提高中华鲟在极端pH条件下的养殖可持续性奠定了理论基础。
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引用次数: 0
Identification of candidate hub genes and key pathways related to growth rate of the red swamp crayfish based on WGCNA analysis 基于WGCNA分析的红沼泽小龙虾生长速率相关候选枢纽基因及关键通路的鉴定
IF 2.2 2区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2026-01-20 DOI: 10.1016/j.cbd.2026.101758
Na Sheng , Yongchuang Li , Yongqing Wang , Zheyan Chen , Xilei Li , Xianji Tao , Jiale Li , Jianbin Feng
The red swamp crayfish, Procambarus clarkii, is a commercially significant crustacean species for aquaculture globally. Growth traits are of great importance for economic efficiency in the aquaculture of the species. However, the molecular mechanisms of regulating growth rate in P. clarkii remain poorly understood. Here, to identify the hub genes and key pathways related to growth rate, transcriptome sequencing and Weighted Gene Co-expression Network Analysis (WGCNA) were conducted on the gill, heart, hepatopancreas, intestine, and muscle from P. clarkii with different growth rate in three full-sib families. A total of 906 differentially expressed genes (DEGs) in the gill (95 up-regulated and 811 down-regulated), 1042 DEGs in the heart (45 up-regulated and 997 down-regulated), 257 DEGs in the hepatopancreas (80 up-regulated and 177 down-regulated), 691 DEGs in the intestine (174 up-regulated and 517 down-regulated), and 158 DEGs in the muscle (30 up-regulated and 128 down-regulated) were identified, respectively. The DEGs were annotated into 101 GO terms, which mainly involved in chitin binding, structural components of the stratum corneum, extracellular region and extracellular space. Nine key pathways including the Wnt signaling pathway, autophagy-animal, phagosome, amino sugar and nucleotide sugar metabolism, TGF-β signaling pathway, drug metabolism-other enzymes, mTOR signaling pathway, lysine degradation, and lysosome pathway were identified based on the KEGG enrichment analysis. A hub module was identified by WGCNA analysis. The hub genes related to structural composition, such as cuticle protein 7-like and pro-resilin, as well as genes involved in various cellular processes, like ataxin-2 homolog were identified based on the PPI network analysis. Overall, the results would provide valuable insights into understanding the molecular regulatory mechanisms of growth rate of P. clarkii.
克氏原螯虾(Procambarus clarkii)是一种具有重要商业价值的甲壳类动物。生长性状对养殖经济效益有重要影响。然而,调控克拉氏杆菌生长速率的分子机制尚不清楚。为了确定与生长速率相关的枢纽基因和关键通路,我们对三个全同胞克氏杆菌家族中不同生长速率的克氏杆菌的鳃、心脏、肝胰腺、肠和肌肉进行了转录组测序和加权基因共表达网络分析(WGCNA)。共鉴定出鳃区906个差异表达基因(95个上调,811个下调)、心脏区1042个差异表达基因(45个上调,997个下调)、肝胰脏区257个差异表达基因(80个上调,177个下调)、肠区691个差异表达基因(174个上调,517个下调)和肌肉区158个差异表达基因(30个上调,128个下调)。这些deg被标注为101个GO术语,主要涉及几丁质结合、角质层结构成分、细胞外区域和细胞外空间。通过KEGG富集分析,鉴定出Wnt信号通路、自噬-动物、吞噬体、氨基糖和核苷酸糖代谢、TGF-β信号通路、药物代谢-其他酶、mTOR信号通路、赖氨酸降解和溶酶体等9条关键通路。通过WGCNA分析确定了一个轮毂模块。通过PPI网络分析,确定了与结构组成相关的枢纽基因,如角质层蛋白7-like和前弹性蛋白,以及参与各种细胞过程的基因,如ataxin-2同源基因。本研究结果将为了解克氏杆菌生长速率的分子调控机制提供有价值的见解。
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引用次数: 0
Hepatic physiological and transcriptomic responses of grass carp (Ctenopharyngodon idella) to long-term salinity stress 草鱼对长期盐度胁迫的肝脏生理和转录组反应
IF 2.2 2区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2026-01-20 DOI: 10.1016/j.cbd.2026.101753
Tao Zhu , Zhu Zhu , Shengjie Li , Jing Tian , Caixia Lei , Jinxing Du , Hongmei Song
Salinity profoundly impacts fish survival, distribution, and physiological functions. Grass carp (Ctenopharyngodon idella), a vital aquaculture species, offers a representative model to investigate salinity adaptation mechanisms, providing crucial theoretical support for aquaculture practices. While the liver is a key metabolic organ in fish salinity adaptation, its specific regulatory role in the high-salinity tolerance of grass carp remains unclear. In this study, we investigated the effects of long-term salinity stress (0parts per thousand [ppt], 4 ppt, and 8 for one month) on grass carp liver. At 4 ppt salinity, mild hepatocyte edema and acinar cell hyperplasia were observed, while at 8 ppt salinity, irregular hepatocyte shapes, dilated hepatic sinusoids, and focal inflammatory cell infiltration were detected. Transcriptome sequencing showed that increasing salinity led to significant upregulation of genes involved in steroid synthesis, lipid metabolism, and cholesterol synthesis, while immune-related gene expression trended downward. Notably, LOC127523424 (carbonic anhydrase 4), igfbp7 (insulin-like growth factor-binding protein 7), and taurine transporter genes (slc6a6b, slc6a6a) all increased with rising salinity, suggesting their involvement in enhancing hyperosmotic stress adaptation. In conclusion, our findings confirm a significant impact of salinity on the grass carp liver. Long-term high-salinity adaptation appears to reduce immunity, while the liver enhances high-salinity tolerance through increased steroid synthesis and metabolism.
盐度对鱼类的生存、分布和生理功能有着深远的影响。草鱼(Ctenopharyngodon idella)是一种重要的水产养殖物种,为研究盐度适应机制提供了有代表性的模型,为水产养殖实践提供了重要的理论支持。虽然肝脏是鱼类盐度适应的关键代谢器官,但其在草鱼高盐度耐受性中的具体调节作用尚不清楚。在本研究中,我们研究了长期盐度胁迫(0‰[ppt]、4‰和8‰持续一个月)对草鱼肝脏的影响。盐度为4 ppt时,肝细胞轻度水肿、腺泡细胞增生;盐度为8 ppt时,肝细胞形态不规则、肝窦扩张、局灶性炎症细胞浸润。转录组测序显示,盐度升高导致类固醇合成、脂质代谢和胆固醇合成相关基因显著上调,而免疫相关基因表达呈下降趋势。值得注意的是,LOC127523424(碳酸酐酶4)、igfbp7(胰岛素样生长因子结合蛋白7)和牛磺酸转运蛋白基因(slc6a6b、slc6a6a)都随着盐度的升高而增加,表明它们参与了高渗胁迫适应的增强。总之,我们的研究结果证实了盐度对草鱼肝脏的显著影响。长期的高盐度适应似乎会降低免疫力,而肝脏通过增加类固醇合成和代谢来增强高盐度耐受性。
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引用次数: 0
Different degrees of environmental high temperature induce varying endoplasmic reticulum stress responses in Apostichopus japonicus 不同程度的环境高温诱导刺参内质网的应激反应不同。
IF 2.2 2区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2026-01-20 DOI: 10.1016/j.cbd.2026.101755
Qiang Wang , Siyi Li , Yu Yang , Rubiao Xie , Lingbin Wang , Weijun Wang , Jianmin Yang , Guohua Sun
Heat temperature caused by changes in the global environment have significant impacts on marine organisms. Apostichopus japonicus (A. japonicus) is an economically important benthic species in China's shallow-sea aquaculture. However, its growth and survival are easily affected by rising seawater temperatures. Therefore, it is necessary to explore its response to environmental high temperature. Endoplasmic reticulum (ER) stress serves as an important regulatory strategy for organisms to respond to environmental changes. It acts as the core hub connecting stress and immunity. In this study, we analyzed the histology, ultrastructure, and transcriptome of the digestive tract of A. japonicus at three temperatures: normal (18 °C), aestivation (25 °C), and lethal (32 °C) temperatures, to explore the role of ER stress in response to high temperature. Histological and ultrastructural results indicate that high temperature caused morphological changes in the digestive tract and that the structure and morphology of the ER exhibit alterations and even varying degrees of damage. A total of 603 and 4615 differentially expressed genes (DEGs) were identified by transcriptome sequencing in the T25-vs-T18 and the T32-vs-T25 group comparisons, respectively. The GO results showed that DEGs were significantly enriched in GO terms related to protein folding, such as chaperone-mediated protein folding in both comparison groups. Additionally, KEGG enrichment analysis showed that both groups activated the pathway of protein processing in the ER and induced the ER stress response. The ER molecular chaperones, including BiP, GRP94, and HSP70, were all upregulated in expression. In addition to the aforementioned ER molecular chaperones, downstream factors in the unfolded protein response, such as S1P, TRAF2, and XBP, were also significantly upregulated in T32-vs-T25 group comparisons, indicating that UPR signaling pathways had enhanced expression. Our findings have characterized the internal molecular regulatory process of A. japonicus under high temperature from the perspective of ER stress and provides clues regarding immune response and homeostatic regulation in invertebrates under environment stress. These findings can provide a reference for the formulation of management measures to mitigate the impact of climate change on aquaculture.
全球环境变化引起的高温对海洋生物有重要影响。Apostichopus japonicus (A. japonicus)是中国浅海养殖中重要的经济底栖物种。然而,它的生长和生存很容易受到海水温度上升的影响。因此,有必要探讨其对环境高温的响应。内质网应激是生物体应对环境变化的重要调控策略。它是连接压力和免疫力的核心枢纽。本研究通过对正常(18°C)、休眠(25°C)和致死(32°C) 3种温度下日本稻(A. japonicus)消化道的组织学、超微结构和转录组进行分析,探讨内质网应激在高温响应中的作用。组织学和超微结构结果表明,高温引起消化道形态学改变,内质网结构和形态发生改变甚至不同程度的损伤。通过转录组测序,T25-vs-T18组和T32-vs-T25组分别鉴定出603个和4615个差异表达基因(deg)。氧化石墨烯结果显示,在两个对照组中,DEGs中与蛋白质折叠相关的氧化石墨烯含量显著增加,如伴侣蛋白介导的蛋白质折叠。此外,KEGG富集分析表明,两组均激活内质网蛋白加工途径,诱导内质网应激反应。内质网分子伴侣BiP、GRP94、HSP70的表达均上调。除了上述ER分子伴侣外,未折叠蛋白反应的下游因子,如S1P、TRAF2和XBP在T32-vs-T25组的比较中也显著上调,表明UPR信号通路的表达增强。我们的研究结果从内质网应激的角度表征了高温下日本刺参的内部分子调控过程,为研究环境应激下无脊椎动物的免疫反应和稳态调控提供了线索。研究结果可为制定缓解气候变化对水产养殖影响的管理措施提供参考。
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引用次数: 0
Transcription dynamics and regulation of heat shock protein genes during stress and development in the estuarine cnidarian Nematostella vectensis 河口刺胞线虫应激和发育过程中热休克蛋白基因的转录动力学和调控。
IF 2.2 2区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2026-01-16 DOI: 10.1016/j.cbd.2026.101754
Janki A. Bhalodi , Joachim M. Surm , Adam M. Reitzel
Heat shock proteins (HSPs) are molecular chaperones that function in protecting cells from proteotoxicity. Eukaryotes have multiple HSPs that localize in the cytoplasm, endoplasmic reticulum (ER), and mitochondria. In cnidarian species, where HSPs are often used as biomarkers of environmental stress, little is known about how particular HSPs vary in copy number, expression, inducibility, and regulation within a species. Here, we characterized the full repertoire of HSP70 and HSP90 genes in an emerging model cnidarian, Nematostella vectensis. We identified five HSP70 and three HSP90 genes, with at least one homolog from each family belonging to the three primary clades based on subcellular localization. Although transcriptional induction remained insignificant by a 10 °C temperature change, two cytosolic HSP70s and one cytosolic HSP90 were significantly upregulated with a 20 °C temperature increase. Most HSPs exhibited similar developmental expression patterns, with elevated expression during the early larval stage followed by reduced expression in the juvenile stage. HSPs showed evidence for differential expression across cell types, with multiple cytosolic and ER HSPs being highly expressed in neuronal and cnidocyte populations. Moreover, the putative promoters of N. vectensis HSPs differed in both the abundance and sequences of regulatory heat shock element motifs, providing a potential mechanism of functional diversification in response to temperature and development. By characterizing expression of all HSP70 and HSP90 genes in this cnidarian, we reveal distinct roles of these core chaperones in the proteostasis response, providing a foundation for future functional studies on contributions of HSPs to cnidarian life cycle and stress resilience.
热休克蛋白(HSPs)是一种具有保护细胞免受蛋白质毒性作用的分子伴侣蛋白。真核生物有多种热休克蛋白,它们分布在细胞质、内质网和线粒体中。在刺胞动物物种中,热休克蛋白经常被用作环境胁迫的生物标志物,但人们对特定热休克蛋白在一个物种内的拷贝数、表达、诱导和调控方面的变化知之甚少。在这里,我们鉴定了一种新型刺胞动物——线虫病的HSP70和HSP90基因的全部序列。我们鉴定了5个HSP70和3个HSP90基因,根据亚细胞定位,每个家族至少有一个同源基因属于三个初级分支。虽然在10℃温度下转录诱导不显著,但在20℃温度升高时,两种细胞质hsp70和一种细胞质HSP90显著上调。大多数热休克蛋白表现出相似的发育表达模式,在幼虫早期表达升高,然后在幼年期表达降低。热休克蛋白在不同细胞类型中表现出差异表达,多种胞质热休克蛋白和内质热休克蛋白在神经元和刺胞细胞群体中高度表达。此外,这些推测的启动子在调节热休克元件的丰度和序列上都存在差异,这为温度和发育下的功能多样化提供了潜在的机制。通过表征所有HSP70和HSP90基因在该刺胞动物中的表达,我们揭示了这些核心伴侣蛋白在蛋白质停滞反应中的不同作用,为进一步研究热休克蛋白对刺胞动物生命周期和应激恢复能力的贡献奠定了基础。
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引用次数: 0
Proteomic analysis reveals the mechanism of cold tolerance in black porgy (Acanthopagrus schlegelii) via ribosome hibernation, metabolic remodeling, and antioxidant coordination 蛋白质组学分析揭示了黑棘虫(Acanthopagrus schlegelii)的耐寒机制,包括核糖体冬眠、代谢重塑和抗氧化协调
IF 2.2 2区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2026-01-15 DOI: 10.1016/j.cbd.2026.101748
Jingyang Li , Ruijian Sun , Tongxuan Zhao , Xiaojian Tang , Bo Gao , Guangping Xu , Yue Wang , Han Yu , Qian Meng , Zhiwei Zhang
Low-temperature stress poses a critical challenge to the overwintering survival of black porgy (Acanthopagrus schlegelii), a commercially important marine fish distributed across the coastal waters of West Pacific region, including the continental shelves of China, Japan, and the Korean Peninsula. To unravel the molecular mechanisms underlying cold adaptation, this study employed quantitative proteomics was employed to analyze hepatic protein profiles of black porgy between three groups: control group (CG, 15 °C), cold-sensitive group (CS, 3.8 °C), and cold-tolerant group (CT, 2.8 °C). A total of 4437 proteins were identified, with 1616 differentially expressed protein (DEPs) detected among the groups. Bioinformatics analyses, including Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment, Gene Set Enrichment Analysis (GSEA), and trend analysis, revealed distinct adaptive strategies between CT and CS groups. The CT group exhibited a coordinated “energy conservation - metabolic remodeling - antioxidation” strategy: (1) significant downregulation of ribosomal subunits and protein export pathways to reduce Adenosine Triphosphate (ATP) consumption from protein synthesis (called “ribosomal hibernation”); (2) upregulation of Peroxisome Proliferator-Activated Receptor (PPAR) signaling pathway and peroxisomal functions to enhance fatty acid β-oxidation and ketone body production, facilitating efficient energy supply; (3) activation of antioxidant systems to mitigate damage induced by reactive oxygen species (ROS). In contrast, the CS group showed dysregulated energy metabolism, characterized by enhanced but inefficient glycolysis, impaired endoplasmic reticulum function, and excessive inflammatory responses, which may contribute to protential proteotoxic stress and metabolic dysfunction. Key DEPs and pathways, such as ribosomal proteins, PPAR family proteins, and peroxisomal enzymes, were identified as protential core regulators of cold tolerance in black porgy. This study provides the first comprehensive proteomic insights into the molecular mechanisms of cold tolerance in black porgy, highlighting the evolutionary significance of energy allocation and metabolic plasticity in teleosts. These findings offer potential molecular markers for breeding cold-tolerant strains, addressing critical challenges in aquaculture sustainability.
黑porgy (Acanthopagrus schlegelii)是一种重要的商业海鱼,分布在西太平洋地区的沿海水域,包括中国、日本和朝鲜半岛的大陆架,低温胁迫对黑porgy的越冬生存构成了严峻的挑战。为了揭示冷适应的分子机制,本研究采用定量蛋白质组学方法分析了三组黑porgy肝脏蛋白谱:对照组(CG, 15°C)、冷敏感组(CS, 3.8°C)和耐寒组(CT, 2.8°C)。共鉴定出4437个蛋白,组间检测到1616个差异表达蛋白(DEPs)。生物信息学分析,包括基因本体(GO)、京都基因与基因组百科全书(KEGG)富集、基因集富集分析(GSEA)和趋势分析,揭示了CT组和CS组之间不同的适应策略。CT组表现出协调的“能量节约-代谢重塑-抗氧化”策略:(1)显著下调核糖体亚基和蛋白质输出途径,以减少蛋白质合成中三磷酸腺苷(ATP)的消耗(称为“核糖体冬眠”);(2)上调过氧化物酶体增殖激活受体(PPAR)信号通路和过氧化物酶体功能,促进脂肪酸β-氧化和酮体生成,促进高效能量供应;(3)激活抗氧化系统以减轻活性氧(ROS)引起的损伤。相比之下,CS组表现出能量代谢失调,表现为糖酵解增强但效率低下,内质网功能受损,炎症反应过度,可能导致潜在的蛋白质毒性应激和代谢功能障碍。核糖体蛋白、PPAR家族蛋白和过氧化物酶体酶等关键dep及其通路被确定为黑豆耐寒性的潜在核心调控因子。本研究首次从蛋白质组学角度全面揭示了硬骨鱼耐冷性的分子机制,强调了硬骨鱼能量分配和代谢可塑性的进化意义。这些发现为培育耐寒菌株提供了潜在的分子标记,解决了水产养殖可持续性的关键挑战。
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引用次数: 0
Comprehensive transcriptomic analysis reveals detoxification gene expansion and adaptive genetic variation in the emerging pest Acanthotomicus suncei 综合转录组学分析揭示了新兴害虫棘虫的解毒基因扩增和适应性遗传变异
IF 2.2 2区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2026-01-13 DOI: 10.1016/j.cbd.2026.101749
Lulu Dai, Jiao Li, Weiyi Pan, Fangyuan Shen, Dejun Hao
The bark beetle Acanthotomicus suncei is an emerging destructive pest of the introduced American sweetgum (Liquidambar styraciflua) in China. The molecular mechanisms underlying its successful adaptation to this exotic host, particularly its ability to overcome plant chemical defenses, remain largely unknown. We conducted a comprehensive transcriptomic analysis of A. suncei. Our assembly yielded 30,557 unigenes (N50 = 2175 bp). We identified a significant expansion of genes within three major detoxification enzyme families: 122 cytochrome P450 monooxygenases (P450s), 75 carboxylesterases (COEs), and 47 glutathione S-transferases (GSTs). This repertoire is notably larger than that of many compared curculionid beetles. Phylogenetic analyses revealed complex evolutionary relationships, with A. suncei clustering closely with Dendroctonus species in some P450 and GST clades, while showing considerable divergence in others. Furthermore, we uncovered abundant genetic variation, with single nucleotide polymorphisms (SNPs) being highly prevalent within the coding sequences of these detoxification genes, especially P450s. Simple sequence repeat (SSR) analysis indicated a predominance of A/T-rich motifs. The remarkable expansion and substantial genetic variation observed in the detoxification gene families of A. suncei provide a compelling molecular basis for its potent detoxification capacity. These findings suggest that an enriched and highly adaptable detoxification system likely facilitates this beetle's successful colonization of the exotic host L. styraciflua. This study offers crucial insights into the molecular mechanisms of host adaptation in an emerging forest pest and identifies potential genetic targets for future management strategies.
suncei树皮甲虫是中国引进的美洲甜(Liquidambar styraciflua)的一种新出现的破坏性害虫。其成功适应这种外来寄主的分子机制,特别是其克服植物化学防御的能力,在很大程度上仍然未知。我们进行了一项全面的转录组学分析。我们的组装得到30,557个unigenes (N50 = 2175 bp)。我们在三个主要的解毒酶家族中发现了显著的基因扩增:122个细胞色素P450单加氧酶(P450s), 75个羧酯酶(COEs)和47个谷胱甘肽s转移酶(GSTs)。这一技能明显比许多相比较的曲头甲虫要大。系统发育分析揭示了复杂的进化关系,在一些P450和GST分支中,suncei与Dendroctonus物种密切聚集,而在其他分支中则表现出相当大的差异。此外,我们发现了丰富的遗传变异,单核苷酸多态性(snp)在这些解毒基因的编码序列中非常普遍,尤其是p450。SSR (Simple sequence repeat)分析表明,a / t富集基序居多。解毒基因家族的显著扩展和大量遗传变异为其强大的解毒能力提供了令人信服的分子基础。这些发现表明,一个丰富和高度适应性的解毒系统可能有助于这种甲虫成功定植外来寄主L. styraciflua。这项研究为新出现的森林害虫宿主适应的分子机制提供了重要见解,并为未来的管理策略确定了潜在的遗传靶点。
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引用次数: 0
Transcriptomics analysis provides insights into the molecular response of the clam Cyclina sinensis to Vibrio infection under heat stress 转录组学分析揭示了热应激条件下中华环蚌对弧菌感染的分子反应
IF 2.2 2区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2026-01-13 DOI: 10.1016/j.cbd.2026.101752
Dehui Sun , Xiuke Ouyang , Ruili Zheng , Jing Cao, Lisha Wei, Meng Xu, Guxin Wang
The heat stress and Vibrio infection are considered as the key factors contributing to the mass mortality of clams in summer. In this study, we explored the combined effects of high temperature and Vibrio infection on the survival of the clam Cyclina sinensis. The clams C. sinensis were separately cultivated at 26 °C (low temperature) or 30 °C (high temperature) and subjected to Vibrio infection experiment. The results showed that dual challenges aggravated the mortality of clam. To further investigate the underlying mechanisms, the hepatopancreas of clam C. sinensis under varying thermal conditions were separately sampled at different infection phases, including pre-infection, 8 days post-infection (dpi) and 12 dpi. Transcriptomic analysis revealed that the dual challenges significantly decreased the expression level of genes related to immunity, antioxidation, and energy metabolism, while upregulating genes associated with apoptosis and endoplasmic reticulum stress compared to the control group (26 °C and non-infection). The functional analysis indicated that downregulated differentially expressed genes (DEGs), identified in the dual challenges groups compared with the control group, were primarily involved in lysosome, phagosome, peroxisome, carbohydrate metabolism and regulation of oxidoreductase activity. The qRT-PCR validation of 15 DEGs corroborated the RNA-seq findings. We further demonstrated that the combined stress increased the content of MDA and decreased the activity of antioxidant enzymes (SOD, CAT) and immune enzymes (ACP, LZM) in hepatopancreas. Taken together, these findings suggest that immunosuppression, oxidative damage and deficiencies in carbohydrate metabolism were potential contributors to the mass summer mortality of clams. This study provides valuable data resources and critical information for revealing the molecular response of C. sinensis to combined stress of high temperature and Vibrio infection.
热应激和弧菌感染被认为是夏季蛤蜊大量死亡的关键因素。在本研究中,我们探讨了高温和弧菌感染对中华圆蛤存活的联合影响。分别在26°C(低温)和30°C(高温)条件下培养中华蛤,进行弧菌感染实验。结果表明,双重攻毒加重了蛤蜊的死亡率。为了进一步研究其潜在机制,我们在不同的感染阶段(感染前、感染后8天和12天)分别采集了不同温度条件下的中华蛤肝胰脏。转录组学分析显示,与对照组(26°C和未感染)相比,双重刺激显著降低了与免疫、抗氧化和能量代谢相关的基因表达水平,同时上调了与凋亡和内质网应激相关的基因表达水平。功能分析表明,与对照组相比,双重刺激组差异表达基因(DEGs)的下调主要涉及溶酶体、吞噬体、过氧化物酶体、碳水化合物代谢和氧化还原酶活性的调节。15个deg的qRT-PCR验证证实了RNA-seq的发现。结果表明,复合应激增加了肝胰腺MDA含量,降低了抗氧化酶(SOD、CAT)和免疫酶(ACP、LZM)活性。综上所述,这些发现表明免疫抑制、氧化损伤和碳水化合物代谢不足是夏季蛤蜊大量死亡的潜在原因。本研究为揭示中华梭菌对高温和弧菌感染联合胁迫的分子响应提供了宝贵的数据资源和关键信息。
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引用次数: 0
Multi-omics dissection of large-size formation in Eriocheir sinensis: Insights from RNA, metabolite profiling, and ceRNA regulatory networks 中华绒螯蟹大尺寸结构的多组学解剖:来自RNA、代谢物谱和ceRNA调控网络的见解
IF 2.2 2区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2026-01-12 DOI: 10.1016/j.cbd.2026.101750
Fujun Xuan , Xinyue Zhang , Jinghao Hu , Xuguang Li , Yuchen Chen , Aiming Zhang , Ruifang Wang , Qian Ren , Tao Wu , Weibing Guan , Yongxu Cheng , Jun Zhou , Rongchen Liu
Eriocheir sinensis (Chinese mitten crab) is a key economic species in China's freshwater aquaculture industry. Individual body size is a critical trait that determines both market price and production profitability. Large-sized crabs exhibit substantial commercial advantages; however, the underlying molecular mechanisms regulating size formation remain poorly understood. In this study, we conducted an integrative multi-omics analysis combining whole-transcriptome data (mRNA, miRNA, and lncRNA) and untargeted metabolomics across two aquaculture cohorts (cohort2023 and cohort2024). Our results revealed a systemic downregulation of glycolysis, the tricarboxylic acid (TCA) cycle, fatty acid oxidation, and glycerol metabolism in large-sized crabs, suggesting a “low consumption–high storage” metabolic strategy. In contrast, pathways related to organismal development, exoskeleton reconstruction, steroid hormone biosynthesis, and nutrient absorption were significantly upregulated, indicating enhanced growth potential and nutrient assimilation efficiency. ceRNA network modeling and cis-acting lncRNA analysis identified multiple core regulatory genes (e.g., PTGS1, TPI1, POR) as targets of complex non-coding RNA interactions involved in body size regulation. Enzyme activity assays for key rate-limiting steps in carbohydrate and lipid catabolism, along with extensive qPCR validation, further corroborated the transcriptomic findings. Taken together, our study provides the first comprehensive multi-omics perspective on the molecular basis of body size differentiation in E. sinensis, proposing a tripartite mechanism involving suppressed catabolism, stimulated growth and morphogenesis, and improved nutrient acquisition. These findings offer theoretical insight into crustacean growth regulation and provide molecular targets to support selective breeding of high-value, large-sized mitten crab strains.
中华绒螯蟹(Eriocheir sinensis)是中国淡水养殖业的重要经济物种。个体尺寸是决定市场价格和生产利润的关键特征。大型螃蟹具有显著的商业优势;然而,调节尺寸形成的潜在分子机制仍然知之甚少。在本研究中,我们结合两个水产养殖队列(cohort2023和cohort2024)的全转录组数据(mRNA, miRNA和lncRNA)和非靶向代谢组学进行了综合多组学分析。我们的研究结果揭示了大蟹糖酵解、三羧酸(TCA)循环、脂肪酸氧化和甘油代谢的系统性下调,表明了一种“低消耗-高储存”的代谢策略。相比之下,与机体发育、外骨骼重建、类固醇激素生物合成和营养吸收相关的途径显著上调,表明生长潜力和营养同化效率增强。ceRNA网络建模和顺式作用lncRNA分析发现,多个核心调控基因(如PTGS1、TPI1、POR)是参与体型调节的复杂非编码RNA相互作用的靶点。碳水化合物和脂质分解代谢中关键限速步骤的酶活性测定,以及广泛的qPCR验证,进一步证实了转录组学的发现。综上所述,我们的研究首次从多组学的角度全面研究了中华赤霉素体型分化的分子基础,提出了抑制分解代谢、促进生长和形态发生以及改善营养获取的三重机制。这些发现为研究甲壳类动物的生长调控提供了理论依据,并为高价值、大型绒螯蟹品系的选择性育种提供了分子靶点。
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引用次数: 0
Comparative transcriptome analysis reveals molecular mechanism of temperature effect on body color of red swamp crayfish (Procambarus clarkii) 比较转录组分析揭示温度对克氏原螯虾体色影响的分子机制
IF 2.2 2区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2026-01-10 DOI: 10.1016/j.cbd.2026.101751
Guoliang Chang , Fanqian Kong , Songyue Gao , Huajie Cai , Long Wang , Huaiyu Ding , Yongxu Cheng , Jiayao Li
The carapace coloration is of great significance to Procambarus clarkii, and the molecular mechanism of temperature affecting carapace color has not yet been reported. In this study, comparative transcriptome was used to study molecular mechanism of temperature-induced changes in carapace coloration of P. clarkii. This study identified many genes (such as tyrosinase, APOD) and signaling pathways (such as tyrosine metabolism) related to pigment accumulation. As an important environmental factor, temperature can change metabolism and accumulation of pigment in P. clarkii by changing expression patterns of related genes and pathways. High temperature can damage transport of related proteins (such as ABC transporters), thus affecting the metabolism of carotenoids and melanin, and finally lead to the change of body color of P. clarkii. Oxidative stress caused by high temperature can destroy pigment metabolism and accumulation, resulting in changes in body color. The results of this study revealed molecular mechanism of temperature affecting body color of P. clarkii, provided basic data for subsequent selective breeding and practical production, and provided new insights for crustacean body color formation and change.
甲壳着色对克氏原螯虾具有重要意义,温度影响甲壳着色的分子机制尚未见报道。本研究采用比较转录组学方法研究温度诱导克氏假单胞菌甲壳颜色变化的分子机制。本研究发现了许多与色素积累相关的基因(如酪氨酸酶、APOD)和信号通路(如酪氨酸代谢)。温度作为一个重要的环境因子,可以通过改变相关基因和途径的表达模式来改变克氏疟原虫色素的代谢和积累。高温会破坏相关蛋白(如ABC转运蛋白)的运输,从而影响类胡萝卜素和黑色素的代谢,最终导致克氏杆菌体色的变化。高温引起的氧化应激会破坏色素的代谢和积累,导致身体颜色的变化。本研究结果揭示了温度影响克拉氏疟原虫体色的分子机制,为后续的选育和实际生产提供了基础数据,并为甲壳类动物体色的形成和变化提供了新的见解。
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Comparative Biochemistry and Physiology D-Genomics & Proteomics
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