Comparative Biochemistry and Physiology D-Genomics & Proteomics最新文献

{"title":"A multi-omics investigation reveals the hepatic response to salinity stress in grass carp (Ctenopharyngodon idella)","authors":"Xiufeng Fang ,&nbsp;Yuexuan Wang ,&nbsp;Renjie Yu ,&nbsp;Dekun Tang ,&nbsp;Zihan Li ,&nbsp;Qiwei Qin ,&nbsp;Shina Wei","doi":"10.1016/j.cbd.2026.101762","DOIUrl":"10.1016/j.cbd.2026.101762","url":null,"abstract":"<div><div>In the context of increasing freshwater scarcity and the effects of climate change on aquatic environments, developing salt-tolerant fish strains has become a critical strategy for sustainable aquaculture. The limited availability of suitable species for saline-water aquaculture poses a significant challenge, severely impacting the development of the fishery economy. This study investigated the effects of 24 h exposure to freshwater, and seawater at salinities of 4, 9 ppt and 12 ppt, on juvenile grass carp (<em>Ctenopharyngodon idella</em>). Following these exposures, liver tissues were collected to assess physiological and biochemical indicators, as well as transcriptional and metabolic responses. Histological examination revealed that liver structure was compromised after 24 h of exposure to 9 ppt and 12 ppt salt stress. Concurrently, we observed a decrease in the levels of Superoxide Dismutase (SOD), whereas the levels of Malondialdehyde (MDA) exhibited an increase. Salinity exposure significantly altered the levels of 120 metabolic products (67% of which were lipid and lipophilic molecules) and the transcriptional expression of 1005 genes. Metabolomic analysis indicated that most of the significantly different metabolites were associated with the metabolism of lipids and amino acids. Transcriptome analysis revealed significant enrichment of 20 metabolic pathways, including glutathione metabolism, lipid digestion and absorption, bile secretion, glycerolipid metabolism, and the tricarboxylic acid cycle. Comprehensive multi-omics analysis revealed significant alterations in key metabolic pathways, including glycerophospholipid metabolism, α-linolenic acid metabolism, histidine metabolism, and β-alanine metabolism, along with several vital genes such as <em>HO-1, NQO1, GCLM,</em> and <em>GSS,</em> under salt stress. These changes closely correlate with variations in cellular membrane lipid composition and antioxidant activity. Further analysis demonstrated that acute salt stress induces oxidative damage in the liver, leading to lipid imbalance and oxidative stress. This is evidenced by impaired antioxidant function and disruptions in amino acid and fatty acid metabolism. Moreover, carnosine synthesis in liver tissue occurs via the activation of histidine and β-alanine metabolic pathways, leading to the upregulation of CNDP2. This process plays a crucial role in regulating lipid metabolism and redox homeostasis, effectively mitigating the damage caused by acute salinity stress. In summary, these findings provide a deeper understanding of the molecular mechanisms underlying salt stress responses in grass carp and offer valuable insights for the breeding of salt-tolerant strains of grass carp.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"58 ","pages":"Article 101762"},"PeriodicalIF":2.2,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146077499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of genes involved in the development of Weberian apparatus in rohu, Labeo rohita (Hamilton, 1822), as revealed by comparative transcriptome analyses","authors":"Nirjharini Priyadarshini ,&nbsp;Priyanka Nandanpawar ,&nbsp;Bismay Sahoo ,&nbsp;Pravati Kumari Mahapatra ,&nbsp;Lakshman Sahoo ,&nbsp;Paramananda Das","doi":"10.1016/j.cbd.2026.101776","DOIUrl":"10.1016/j.cbd.2026.101776","url":null,"abstract":"<div><div>Ostariophysi has a history of successful adaptation to freshwater environments due to the evolution of a unique morphological structure, i.e. Weberian apparatus (WA). This structure transfers sound waves from the swim bladder to the inner ear by forming a physical linkage that leads to enhanced hearing sensitivity. However, there are very few reports on genes specifically linked to this modified structure in fish. In this study, we compared Weberian ossicles, the modified vertebrae, and unmodified vertebrae transcriptomes of representative species from ostariophysi and non-ostariophysi by mRNA sequencing. A total of 21 and 25 million high-quality reads were generated from <em>Labeo rohita</em> and <em>Oreochromis niloticus</em>, respectively, and differential expression analysis identified 161 upregulated and 98 down-regulated transcripts (&gt;2 fold change) between modified and unmodified vertebrae. Functional annotation revealed their significant involvement in biological functions such as energy metabolism, osteoblast proliferation, differentiation, and matrix mineralization related to bone development. A total of 41 miRNA target interactions were found to be associated with differentially expressed transcripts (<em>p</em> <em>&lt;</em> <em>0.05</em>). Fourteen hub genes were identified and categorized into six major GO terms: endoplasmic reticulum, large ribosomal subunit, rRNA binding, translation and metabolic process. Ontogenic expression of selected genes linked to ossification showed elevated expression till 3 days post-hatch (dph). A novel gene, LrOSSP1 (Ossicle Secretory Signal Protein 1), reported for the first time, exclusively found in ostariophysi, demonstrated significant up-regulation in the earliest stages of larval development in this study. This is the first report of its kind in <em>Labeo rohita</em> and will be helpful in understanding the molecular underplay of modified vertebrae in ostariophysans.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"58 ","pages":"Article 101776"},"PeriodicalIF":2.2,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146168553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptomics analysis provides insights into the molecular response of the clam Cyclina sinensis to Vibrio infection under heat stress","authors":"Dehui Sun ,&nbsp;Xiuke Ouyang ,&nbsp;Ruili Zheng ,&nbsp;Jing Cao,&nbsp;Lisha Wei,&nbsp;Meng Xu,&nbsp;Guxin Wang","doi":"10.1016/j.cbd.2026.101752","DOIUrl":"10.1016/j.cbd.2026.101752","url":null,"abstract":"<div><div>The heat stress and <em>Vibrio</em> infection are considered as the key factors contributing to the mass mortality of clams in summer. In this study, we explored the combined effects of high temperature and <em>Vibrio</em> infection on the survival of the clam <em>Cyclina sinensis</em>. The clams <em>C. sinensis</em> were separately cultivated at 26 °C (low temperature) or 30 °C (high temperature) and subjected to <em>V</em>ibrio infection experiment. The results showed that dual challenges aggravated the mortality of clam. To further investigate the underlying mechanisms, the hepatopancreas of clam <em>C. sinensis</em> under varying thermal conditions were separately sampled at different infection phases, including pre-infection, 8 days post-infection (dpi) and 12 dpi. Transcriptomic analysis revealed that the dual challenges significantly decreased the expression level of genes related to immunity, antioxidation, and energy metabolism, while upregulating genes associated with apoptosis and endoplasmic reticulum stress compared to the control group (26 °C and non-infection). The functional analysis indicated that downregulated differentially expressed genes (DEGs), identified in the dual challenges groups compared with the control group, were primarily involved in lysosome, phagosome, peroxisome, carbohydrate metabolism and regulation of oxidoreductase activity. The qRT-PCR validation of 15 DEGs corroborated the RNA-seq findings. We further demonstrated that the combined stress increased the content of MDA and decreased the activity of antioxidant enzymes (SOD, CAT) and immune enzymes (ACP, LZM) in hepatopancreas. Taken together, these findings suggest that immunosuppression, oxidative damage and deficiencies in carbohydrate metabolism were potential contributors to the mass summer mortality of clams. This study provides valuable data resources and critical information for revealing the molecular response of <em>C. sinensis</em> to combined stress of high temperature and <em>Vibrio</em> infection.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"58 ","pages":"Article 101752"},"PeriodicalIF":2.2,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146022425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide identification and characterization of HSP90, HSP60 and HSP40 family genes in Riptortus pedestris (Hemiptera: Alydidae)","authors":"Wenhao Dong ,&nbsp;Huaijun Xue ,&nbsp;Yipeng Ren","doi":"10.1016/j.cbd.2026.101745","DOIUrl":"10.1016/j.cbd.2026.101745","url":null,"abstract":"<div><div>To the best of our knowledge, heat shock proteins (HSPs) serve as molecular chaperones and play vital roles in providing protection under numerous physiological processes and environmental stressors, such as temperature, mechanical injury, chemical agents, and so on. Although their functional roles in hemipteran insects have been identified, comprehensive genome-wide identification and characterization of <em>HSP</em> family genes remain poorly understood in heteropteran pests, especially in <em>R. pedestris</em>, which has led to significant and widespread issues with soybean quality and yield through its sucking stylet, thus inducing a staygreen-like syndrome. In this study, four <em>HSP90</em>, fourteen <em>HSP60</em> and twenty-six <em>HSP40</em> family genes were obtained from the <em>R. pedestris</em> genome. Next, phylogenetic analysis, combined with conserved and domain characterization, supported our classification results. Chromosomal mapping indicated that all these <em>HSP</em> genes are distributed across the six chromosomes of <em>R. pedestris</em>, and a Ka/Ks ratio of &lt;1 provides essential information for purifying selection acting on two duplicated <em>Hsp90</em> genes in <em>R. pedestris</em>. Through data mining of transcriptome data, we obtained the expression patterns of three <em>HSP</em> family genes across all developmental stages and analyzed their expression differences during changes in feeding conditions in the whole body and gut of <em>R. pedestris</em>, respectively. Finally, a miRNA–mRNA interaction network of four significantly differentially expressed (DE) <em>HSPs</em> and corresponding regulatory miRNAs was constructed, of which one miRNA, novel-miRNA-927-3p, exhibited significant downregulation and combined with significantly DE <em>Hsp60a</em> and <em>DnaJA1</em> in one comparison group, suggesting their underlying relationships in response to antibiotic feeding in the <em>R. pedestris</em> gut. Overall, these findings provide valuable insights into how the three types of <em>HSP</em> genes in <em>R. pedestris</em> are involved in developmental and environmental adaptation at the transcriptional and posttranscriptional levels.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"58 ","pages":"Article 101745"},"PeriodicalIF":2.2,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145925935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diversity of ligand-gated ion channels in free-living and parasitic copepods (Crustacea)","authors":"F. Neptalí Morales-Serna ,&nbsp;Ivana Ramos-de la Cruz ,&nbsp;Alejandra García-Gasca ,&nbsp;Miguel Betancourt-Lozano ,&nbsp;Jesús Briones-Mendoza ,&nbsp;Josué Pinto-Gualpa ,&nbsp;Luis A. Yañez-Guerra ,&nbsp;Víctor Hugo Caña-Bozada","doi":"10.1016/j.cbd.2025.101741","DOIUrl":"10.1016/j.cbd.2025.101741","url":null,"abstract":"<div><div>Ligand-gated ion channels (LGICs) are essential mediators of synaptic transmission and sensory processing in animals, yet their diversity and evolution in copepods remain poorly understood. Copepods are among the most abundant metazoans in marine ecosystems and also occur across a wide range of salinity regimes, including freshwater habitats. They play central roles in food webs, biogeochemical cycling, and host-parasite interactions, with some parasitic taxa having significant impacts on aquaculture. In this study, we conducted a comprehensive analysis of LGICs across 20 copepod species (13 free-living and 7 parasitic) spanning four orders, Calanoida, Cyclopoida, Harpacticoida, and Siphonostomatoida, using transcriptomic and expressed sequence tag (EST) datasets. We identified 6456 putative LGICs belonging to four major families: Cys-loop, ionotropic glutamate receptors (iGluRs), P2X, and ASIC/Deg/ENaC. Phylogenetic and clustering analyses revealed both conserved and lineage-specific patterns, with approximately 20 % of LGICs showing taxonomic restriction to copepods, particularly within the ASIC/Deg/ENaC family. Notably, P2X receptors, previously reported as largely absent in arthropods, were consistently detected across copepod species, suggesting that this family is comparatively well represented in the group. We also identified multiple receptors associated with environmental sensing, including ionotropic receptors (IRs), shared across free-living species, as well as potential pharmacological targets such as GluCl and GABA receptors in parasitic taxa. Our findings highlight the evolutionary complexity of LGICs in copepods and provide a foundation for future research on their roles in environmental adaptation, and chemosensory biology, with potential pharmacological applications in aquaculture.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"58 ","pages":"Article 101741"},"PeriodicalIF":2.2,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145914212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive identification and expression profiling of piRNA pathway genes in the Pacific oyster Crassostrea gigas","authors":"Xuan Zhao ,&nbsp;Yaru Zhou ,&nbsp;Hong Yu ,&nbsp;Qi Li","doi":"10.1016/j.cbd.2025.101739","DOIUrl":"10.1016/j.cbd.2025.101739","url":null,"abstract":"<div><div>PIWI-interacting RNAs (piRNAs) and their associated proteins are key regulators of germline development and genome defense, but their roles in mollusks remain largely unexplored. In this study, we systematically identified 28 piRNA pathway genes in the Pacific oyster <em>Crassostrea gigas</em> and characterized their genomic composition and expression profiles. The oyster piRNA gene repertoire exhibits a mosaic-like architecture, combining deeply conserved components with lineage-specific features, including the presence of vertebrate-type transcription factors such as A-MYB, the absence of the insect-specific RDC complex, and the retention of the exonuclease MUT7, which has been lost in several vertebrate and insect lineages. Transcriptome analyses revealed pronounced tissue-, stage-, and sex-biased expression patterns, consistent with this composite regulatory configuration. Several key factors, including <em>CgPiwil1</em>, <em>CgGtsf1</em>, and <em>CgTdrd5</em> exhibit female-biased expression peaking at the maturation stage of the gonads, whereas <em>CgYthdc2</em>, <em>CgMyb</em>, and <em>CgHsp83</em> show preferential expression in male gonads. Furthermore, <em>CgHsp83</em> displays high expression in gills, suggesting potential multifunctional roles beyond germline regulation. qPCR validation of representative genes confirmed the transcriptome-based patterns. These findings provide the first comprehensive overview of the piRNA pathway in mollusks, reveal both conserved and divergent features compared to model animals, and establish a foundation for future functional studies on germline regulation in bivalves.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"58 ","pages":"Article 101739"},"PeriodicalIF":2.2,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145884358","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide identification and characterization of the HSP gene superfamily in the Ivory Shell (Babylonia areolata)","authors":"Deng Fu ,&nbsp;Yingyin Wu ,&nbsp;Xin Hong ,&nbsp;Yanyun Zhu ,&nbsp;Querui Luo ,&nbsp;Guanyi Li ,&nbsp;Kun Tian ,&nbsp;Xing Zheng ,&nbsp;Chunsheng Liu ,&nbsp;Shifeng Wang ,&nbsp;Aiming Wang ,&nbsp;Zhifeng Gu ,&nbsp;Feng Yu","doi":"10.1016/j.cbd.2025.101668","DOIUrl":"10.1016/j.cbd.2025.101668","url":null,"abstract":"<div><div>Heat shock proteins (HSPs) are highly conserved molecular chaperones that play essential roles in biological growth, development, and responses to environmental stress. To investigate the functions of HSPs in <em>Babylonia areolata</em> (BaHsps) under heat stress, a total of 68 BaHsp genes were identified at the genome-wide level using bioinformatics approaches. The phylogenetic tree, gene structure, chromosome location and protein physicochemical properties were predicted, and the expression patterns of BaHsp genes in different tissues under heat stress were analyzed and characterized by Quantitative Real-time polymerase chain reaction method (qPCR). The results revealed significant variation in the number and arrangement of introns among different BaHsp genes, with introns unevenly distributed across 26 chromosomes. Gene duplication analysis indicated tandem duplication events in the <em>BaHsp20</em>, <em>BaHsp40</em> and <em>BaHsp90</em>, suggesting that these events contributed to the expansion and functional diversification of the BaHsp gene families. RT-qPCR results demonstrated clear tissue-specific expression of BaHsp genes, with overall expression levels being relatively high in gill tissue. Under heat stress, expression of BaHsp genes in gill tissue increased with rising temperatures. Temporal expression analysis further showed that most genes were rapidly upregulated at the onset of heat stress, followed by a gradual decline, exhibiting a dynamic pattern of “increase-then-decrease”. This study provides a fundamental reference for further investigations into the molecular mechanisms of HSP gene family response to heat stress in <em>B. areolata.</em></div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"57 ","pages":"Article 101668"},"PeriodicalIF":2.2,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145425806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive analysis of metabolomic and transcriptomic provides insights on the phospholipid regulating LC-PUFAs accumulation in the gonads of sea urchin Strongylocentrotus intermedius","authors":"Huinan Zuo,&nbsp;Lu Tang,&nbsp;Weixiao Di,&nbsp;Feng Zhang,&nbsp;Yanchang Ning,&nbsp;Panke Gong,&nbsp;Dan Gou,&nbsp;Jun Ding,&nbsp;Yaqing Chang,&nbsp;Rantao Zuo","doi":"10.1016/j.cbd.2025.101657","DOIUrl":"10.1016/j.cbd.2025.101657","url":null,"abstract":"<div><div>Phospholipids (PLs) are essential for long-chain polyunsaturated fatty acids (LC-PUFAs) accumulation in the gonads of <em>Strongylocentrotus intermedius</em>. However, the mechanism remains to be clarified. Based on previous studies, we hypothesized that <em>S. intermedius</em> might enhance LC-PUFAs accumulation in gonads via PL transport, PL remodeling, or de novo synthesis. Therefore, we formulated three feeds with different PLs and oil sources (palm oil + soybean lecithin (PO + SL), fish oil + soybean lecithin (FO + SL) and palm oil + krill oil (PO + KO)) to test the hypothesis. Each feed was randomly fed three tanks of <em>S. intermedius</em> for 60 days. Then, growth rate, gonad yield and development were monitored and followed by comprehensive analysis of metabolomic and transcriptomic in digestive tract and gonad. The results showed no significant difference in survival, growth rate and gonad yield among the dietary groups. The gonad development among the dietary groups was only significantly observed in male individuals, with the spermatocytes of <em>S. intermedius</em> fed PO + KO and FO + SL thicker and denser than those fed PO + SL. Metabolomic analysis showed that LC-PUFAs were mainly distributed at the sn-2 position of glycerolphospholipid (GP) and glycerolipids (GL), with LC-PUFAs possessing more unsaturated and longer carbon chains in the gonads than those in the digestive tract. The relative contents of LC-PUFAs in GP and GL were highest in the gonads of <em>S. intermedius</em> fed PO + KO and FO + SL, respectively. <em>S. intermedius</em> fed PO + SL showed unpredictable contents of LC-PUFAs at the sn-2 position of GP and GL deposited in their gonads, although no LC-PUFAs were present in this feed. The differential metabolites (DMs) between each comparison group were all enriched in steroid hormone synthesis pathway in digestive tract and gonads of <em>S. intermedius</em>. Transcriptomic analysis showed that the differential expressed genes (DEGs) between each comparison group were enriched in GO terms like phospholipid transport and fatty acid metabolism, and KEGG pathways fatty acid metabolism and linoleic acid metabolism. Notably, DMs in the digestive tract and DEGs in the gonads were all enriched in biosynthesis of unsaturated fatty acids pathway. Based on these results, it suggested that PLs have a direct promoting effect on LC-PUFA synthesis. Furthermore, PLs were hypothesized to facilitate LC-PUFA transport via PL remodeling and cholesterol co-transport mechanisms, thus enhancing LC-PUFAs accumulation in the gonads of <em>S. intermedius</em>. These findings provided insights into the mechanism of PLs regulating the gonad growth and development of <em>S. intermedius</em>.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"57 ","pages":"Article 101657"},"PeriodicalIF":2.2,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145454243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quercetin enhances antioxidant defense and modulates immune homeostasis in mandarin fish (Siniperca chuatsi): Insights from biochemical and transcriptomic analyses","authors":"Zihan Zhou ,&nbsp;Qichen Jiang ,&nbsp;Mingming Han ,&nbsp;Chenxi Zhu ,&nbsp;Tian Zhu ,&nbsp;Yi Juin Tay ,&nbsp;Guoxing Liu","doi":"10.1016/j.cbd.2025.101690","DOIUrl":"10.1016/j.cbd.2025.101690","url":null,"abstract":"<div><div>Revealing the molecular basis of stress resistance in aquaculture species is the key to achieving sustainable breeding strategies. Based on the two-species complementation strategy, this study systematically evaluated the cross-species protection of natural flavonoid quercetin in mandarin fish (<em>Siniperca chuatsi</em>) and zebrafish (<em>Danio rerio</em>). In this study, the effects of quercetin on the transcription and antioxidant capacity of <em>S. chuatsi</em> gene and the antioxidant capacity of <em>D. rerio</em> were tested. <em>S. chuatsi</em> and <em>D. rerio</em> were immersed in four concentrations (0, 1, 2.5, 5.0 mg/L) of quercetin for 28 days. At 2.5 mg/L, quercetin boosted antioxidant defenses by elevating SOD activity and lowering lipid peroxidation, while modulating immune homeostasis in a dose-dependent manner. Although the two fish species showed a consistent trend in oxidative stress indicators, there were significant species differences in their immune responses. Transcriptome results revealed that <em>S. chuatsi</em> activated key functional modules such as cell structure remodeling, transmembrane transport and protein folding under quercetin intervention, and was enriched in signal transduction, nutrient absorption and fat metabolism pathways, forming a multi-level and synergistically regulated stress defense network. The results show that quercetin can mediate a set of evolutionarily conserved systemic protection mechanisms and has the potential to be used as a functional molecule for aquaculture stress resistance breeding.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"57 ","pages":"Article 101690"},"PeriodicalIF":2.2,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145552345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evolution of the animal globin superfamily: Insights from the blood clam Anadara granosa","authors":"Weifeng Zhang ,&nbsp;Tiancheng Chen ,&nbsp;Yazhuo Hu ,&nbsp;Xizhi Shi ,&nbsp;Yongbo Bao","doi":"10.1016/j.cbd.2025.101703","DOIUrl":"10.1016/j.cbd.2025.101703","url":null,"abstract":"<div><div>In recent decades, various globin groups have been identified and characterized in vertebrates, while studies on invertebrates remain limited. Therefore, we conducted this study to explore the repertoire, evolution, and functions of globin genes in the blood clam <em>Anadara granosa</em>, an economically significant bivalve known for its hemoglobin. A total of 31 globin genes were identified, driven by tandem gene duplications that played a pivotal role in their expansion. Phylogenetic analysis identifies two previously unreported basal clades, provisionally named cluster A and B, alongside the well-known ancient globin groups neuroglobin, androglobin, globin X, and globin X-like. This suggests that invertebrates may have retained a more complete ancestral globin gene repertoire compared to vertebrates, and that the globin gene repertoire in the last common ancestor of vertebrates and invertebrates was more diverse than previously hypothesized. Protein structural analyses indicate that evolutionary changes in hemoglobin's oxygen-transport function may be driven by structural alterations in the CD region and EF helices or substitutions at select residues therein. Furthermore, the ancient globin groups exhibit widespread N-myristoylation and 3C-palmitoylation modifications, indicating their potential membrane-associated ancestral functions. Transcriptome analysis and hypoxia stress experiments indicate that globins are involved in the development and hypoxia tolerance of <em>A. granosa</em>. The pentacoordinate heme in animal globins likely switched from a hexacoordinate form, possibly associated with the evolution of oxygen-carrying functionality. This study expands our understanding of the globin superfamily's structure, function, and evolution, particularly in mollusks.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"57 ","pages":"Article 101703"},"PeriodicalIF":2.2,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145673128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}