Comparative Biochemistry and Physiology D-Genomics & Proteomics最新文献

{"title":"Estrone exposure-induced feminization in male Siniperca chuatsi: A multidimensional study on sex reversal, liver damage, and changes in gene expression","authors":"Kaichun Chen ,&nbsp;Weibin Li ,&nbsp;Weijian Chen ,&nbsp;Shengyue Lin ,&nbsp;Ziyan Deng ,&nbsp;Guojun Cai ,&nbsp;Qiang Li ,&nbsp;Chong Han","doi":"10.1016/j.cbd.2025.101705","DOIUrl":"10.1016/j.cbd.2025.101705","url":null,"abstract":"<div><div>Estrone (E1) is one of the primary natural estrogens found in aquatic environments and has the potential to impact the reproductive and endocrine systems of fish. To evaluate the impact of E1 on <em>Siniperca chuatsi</em>, in this study, male <em>S. chuatsi</em> were exposed to E1 at concentrations of 0.0, 0.01, 0.1, and 1.0 μg/L for 60 consecutive days. Subsequently, we analyzed the gonadal histology, hepatic histology, antioxidant enzyme activities, and transcriptomic analysis of the fish. Histological examination of the gonads revealed that high concentrations of E1 can induce the transformation of testes into ovaries in male <em>S.chuatsi</em>, with an induction rate of 80 %. Moreover, significant changes in the levels of the androgen 11-ketotestosterone and the estrogen were observed at high concentrations. Hepatic histology revealed that E1 exposure led to vacuolization of hepatocytes, pyknosis, and inflammation in the liver. Additionally, exposure to 1.0 μg/L E1 significantly increased the activities of SOD (superoxide dismutase) and T-AOC (total antioxidant capacity) in the liver. Transcriptomic analysis of the liver identified several genes significantly associated with sex differentiation, including <em>vtg1</em>, <em>cyp19a</em>, <em>hsd17β3</em>, and <em>esr1</em>, among others. GO and KEGG enrichment analyses suggested that E1 exposure significantly altered the level of lipid metabolism in the liver of male <em>S. chuatsi</em>. In summary, these results indicate that E1 exposure not only causes oxidative damage to the liver but also induces alterations in hepatic steroid hormone synthesis and lipid metabolism pathways, which are indicative of feminizing endocrine effects, thereby leading to the feminization of male <em>S.chuatsi</em>.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"58 ","pages":"Article 101705"},"PeriodicalIF":2.2,"publicationDate":"2025-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145658889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptomic analysis of gill tissues in blunt snout bream (Megalobrama amblycephala) under hypoxia and bacterial infection","authors":"Anicet Philippe Mane Sany ,&nbsp;Songlin Chen ,&nbsp;Qianqian Qin ,&nbsp;Jinjin Luo ,&nbsp;Han Wang ,&nbsp;Loic Kemmadzong Foning ,&nbsp;Addise Kerebih ,&nbsp;Guodong Zheng ,&nbsp;Shuming Zou","doi":"10.1016/j.cbd.2025.101707","DOIUrl":"10.1016/j.cbd.2025.101707","url":null,"abstract":"<div><div>Blunt snout bream (<em>Megalobrama amblycephala</em>) is an important commercial freshwater fish species in China's aquaculture system. It is unknown how the interaction of bacterial infections and dissolved oxygen concentration affects the gene expression and physiological function of the gill tissues in this fish species. Therefore, fish were exposed to hypoxic conditions and challenged with <em>Aeromonas hydrophila</em>. Then, fish were classified into resistant (H-RMA) and sensitive (H-SMA) groups based on their survival outcomes. Gill tissues were collected for RNA-Seq and functional analysis. A total of 36,774 expressed genes, encompassing 33,852 known genes and 2922 novel genes were identified and around 93 % of these genes were correctly mapped to the reference genome. A comparative expression analysis between H-RMA and H-SMA fish revealed 5482 differentially expressed genes (DEGs) encompassing 2557 up-regulated and 2925 down-regulated genes. The examination of KEGG pathways revealed significant enrichment in DNA replication, proteasome, cell cycle, mismatch repair, oxidative phosphorylation and cellular senescence. Obviously structural alterations were observed in the gill tissues, resulting from the compounded impact of hypoxic stress and bacterial infection. Moreover, antioxidant enzymes (SOD, CAT) and immune enzyme activity modulation (ACP and AKP) were significantly (<em>p</em> &lt; 0.05) changed between H-RMA and H-SMA groups. Our findings suggest a strong connection between cell cycle arrest and cellular senescence, indicating that resistance to these environmental stresses may depend on the capacity of the cellular senescence pathway to coordinate an immune response localized in the gills.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"58 ","pages":"Article 101707"},"PeriodicalIF":2.2,"publicationDate":"2025-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145685743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A multi-omics atlas of testicular development in Leiocassis longirostris: dynamic regulation of spermatogenesis","authors":"Feng Qin ,&nbsp;Fan Liu ,&nbsp;Qi Cao ,&nbsp;Zhen Wei ,&nbsp;Hujun Gao ,&nbsp;Wei Zheng ,&nbsp;Zhenlin Ke ,&nbsp;Yinlin Xiong ,&nbsp;Hui Luo ,&nbsp;Ronghua Wu ,&nbsp;Zhongwei Wang ,&nbsp;Hua Ye","doi":"10.1016/j.cbd.2025.101708","DOIUrl":"10.1016/j.cbd.2025.101708","url":null,"abstract":"<div><div>The Chinese longsnout catfish (<em>Leiocassis longirostris</em>) is a commercially valuable freshwater species in China. We elucidated molecular mechanisms underlying testicular development of <em>L. longirostris</em> across five stages (stages I to V) through integrated transcriptomic and proteomic analyses, which is crucial for enhancing its sperm quality and efficient reproduction. Enrichment analyses identified several key pathways as essential for testicular development, including TGF-β signaling, Wnt signaling, ECM-receptor interaction, ferroptosis, cell cycle regulation, and ubiquitin-mediated proteolysis. Gene Ontology (GO) enrichment highlighted core biological processes such as germ cell proliferation, differentiation, meiotic progression, and spermatogenesis regulation involved in development. Notably, qPCR validation showed peak expression levels of <em>wnt7a</em>, <em>pax6,</em> and <em>kiss1r</em> at distinct spermatogenic phases (<em>p</em> &lt; 0.01), suggesting their potential as temporal biomarkers for identification of development stages. Furthermore, protein-protein interaction (PPI) analyses revealed C-type lysozyme (LysC) as a potential regulatory factor, with peak expression at stages I and III, possibly linking testicular immunity and reproductive processes. These findings elucidate the molecular mechanisms of testicular development and provide insights for developing efficient artificial breeding strategies for <em>L. longirostris</em>.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"58 ","pages":"Article 101708"},"PeriodicalIF":2.2,"publicationDate":"2025-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145685749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evolution of the animal globin superfamily: Insights from the blood clam Anadara granosa","authors":"Weifeng Zhang ,&nbsp;Tiancheng Chen ,&nbsp;Yazhuo Hu ,&nbsp;Xizhi Shi ,&nbsp;Yongbo Bao","doi":"10.1016/j.cbd.2025.101703","DOIUrl":"10.1016/j.cbd.2025.101703","url":null,"abstract":"<div><div>In recent decades, various globin groups have been identified and characterized in vertebrates, while studies on invertebrates remain limited. Therefore, we conducted this study to explore the repertoire, evolution, and functions of globin genes in the blood clam <em>Anadara granosa</em>, an economically significant bivalve known for its hemoglobin. A total of 31 globin genes were identified, driven by tandem gene duplications that played a pivotal role in their expansion. Phylogenetic analysis identifies two previously unreported basal clades, provisionally named cluster A and B, alongside the well-known ancient globin groups neuroglobin, androglobin, globin X, and globin X-like. This suggests that invertebrates may have retained a more complete ancestral globin gene repertoire compared to vertebrates, and that the globin gene repertoire in the last common ancestor of vertebrates and invertebrates was more diverse than previously hypothesized. Protein structural analyses indicate that evolutionary changes in hemoglobin's oxygen-transport function may be driven by structural alterations in the CD region and EF helices or substitutions at select residues therein. Furthermore, the ancient globin groups exhibit widespread N-myristoylation and 3C-palmitoylation modifications, indicating their potential membrane-associated ancestral functions. Transcriptome analysis and hypoxia stress experiments indicate that globins are involved in the development and hypoxia tolerance of <em>A. granosa</em>. The pentacoordinate heme in animal globins likely switched from a hexacoordinate form, possibly associated with the evolution of oxygen-carrying functionality. This study expands our understanding of the globin superfamily's structure, function, and evolution, particularly in mollusks.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"57 ","pages":"Article 101703"},"PeriodicalIF":2.2,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145673128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptomic profiling of Helice tientsinensis reveals its adaptation mechanisms to different salinity levels","authors":"Lijie Cui ,&nbsp;Sijia Hao ,&nbsp;Yayun Guan ,&nbsp;Zhuofan Chen ,&nbsp;Yan Wang ,&nbsp;Zhengfei Wang","doi":"10.1016/j.cbd.2025.101700","DOIUrl":"10.1016/j.cbd.2025.101700","url":null,"abstract":"<div><div><em>Helice tientsinensis</em> is a typical species in the intertidal ecosystem, which is easily affected by fluctuations in salinity. This study systematically investigated the physiological and molecular responses of <em>H. tientsinensis</em> under freshwater (0 ‰), low-salinity (15 ‰, control group), and high salinity (30 ‰) stress conditions at 24 and 48 h, using histological observation, enzyme activity detection, and transcriptome sequencing. The results showed that there were relatively few differentially expressed genes between different salinity groups, indicating that <em>H. tientsinensis</em> has a certain degree of adaptability to salinity fluctuations, which is consistent with its intertidal lifestyle. The gill tissue shows structural damage in the freshwater environment, and immune-related genes were downregulated, indicating that freshwater stress damages the gill tissue and suppresses the immune response. At the same time, <em>H. tientsinensis</em> responds to salinity stress through strategies related to energy metabolism, such as enhancing glycolysis, lipid metabolism, and purine synthesis. The study further reveals that <em>H. tientsinensis</em> adopts a tissue-specific osmoregulation strategy: osmoregulation-related genes (e.g., <em>SLC34A</em>, <em>nptA</em>) are significantly upregulated in gill tissue to enhance ion transport efficiency; in contrast, ion transport-related osmoregulation genes (e.g., <em>ABCA1</em>, <em>ABCC2</em>, <em>ABCC3</em>) are downregulated in the hepatopancreas to reduce metabolic load by saving energy consumption. The gene expression patterns of the two tissues act synergistically to maintain the overall osmotic balance homeostasis of the organism. The purpose of this study was to systematically elucidate the physiological and molecular mechanisms underlying the adaptation of <em>H. tientsinensis</em> to different salinity stresses, thereby filling the gap in current research on its molecular response to salinity changes and providing molecular evidence for the environmental adaptation of intertidal crustaceans.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"58 ","pages":"Article 101700"},"PeriodicalIF":2.2,"publicationDate":"2025-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145738492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aberrant body coloration in captive Yellow River carp (Cyprinus carpio haematopterus): A genomic perspective from whole-genome resequencing and bulk segregant analysis","authors":"Ying Liu ,&nbsp;Le Chang ,&nbsp;Lingran Wang ,&nbsp;Chang Liu ,&nbsp;Di Feng ,&nbsp;Lei Wang","doi":"10.1016/j.cbd.2025.101702","DOIUrl":"10.1016/j.cbd.2025.101702","url":null,"abstract":"<div><div>To elucidate the genetic mechanism underlying body color variation within a single breeding family of captive Yellow River carp (<em>Cyprinus carpio haematopterus</em>), individuals exhibiting normal and aberrant pigmentation from the same concrete pond were selected for analysis. Using bulked segregant analysis combined with whole-genome resequencing, DNA pools were constructed from progeny with extreme phenotypes for sequencing. SNP variation detection followed by linkage analysis identified a candidate region on chromosome 25, spanning approximately 10.1 Mb (from 15,881,284 bp to 26,645,798 bp). Gene Ontology (GO) enrichment analysis revealed four candidate genes potentially associated with aberrant pigmentation: <em>dkk1</em>, <em>kndc1, lyst</em>, and <em>opn3</em>. Based on the candidate genes' mutation sites, certain primers were created, and the PCR products were then sequenced. Notably, a mutation was detected at the 8th bp of the <em>opn3</em> cDNA corresponding to transcript HHLG25g0668 in the aberrant-color offspring. At this position, 81.25 % of wild-type individuals carried the cytosine (C) allele, whereas all mutant individuals carried an adenine (A) substitution, resulting in an amino acid change from serine to tyrosine at position 3 of the protein. This suggests that <em>opn3</em> may play a key role in the development of abnormal pigmentation in Yellow River carp. These findings provide a theoretical foundation for further functional studies of pigmentation-related genes and the genetic improvement of novel germplasm in Yellow River carp.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"58 ","pages":"Article 101702"},"PeriodicalIF":2.2,"publicationDate":"2025-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145685745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptomic analysis of post-mating neural reprogramming and its coordinated control of ovarian development in the mud crab Scylla paramamosain","authors":"Yang Yanan ,&nbsp;Shao Shucheng ,&nbsp;Bao Chenchang ,&nbsp;Cui Zhaoxia","doi":"10.1016/j.cbd.2025.101696","DOIUrl":"10.1016/j.cbd.2025.101696","url":null,"abstract":"<div><div>The cerebral ganglion and eyestalk are pivotal regulators of reproduction in decapod crustaceans. To understand how mating drives ovarian development, we performed a comprehensive transcriptomic analysis of these neural tissues in the mud crab, <em>Scylla paramamosain</em>. Our findings reveal that mating induces significant molecular reprogramming, coordinating post-mating neuroendocrine functions. Functional specialization was also evident: the cerebral ganglion showed enrichment in pathways for tight junctions and cytoskeletal reorganization, while the eyestalk primarily activated second messenger signaling crucial for neurohormone release. Among the differentially expressed genes (DEGs), <em>Follistatin-like</em> and <em>TDRD1</em> were implicated in neuroendocrine mechanisms that promote vitellogenesis. Other DEGs related to synaptic transmission (e.g., <em>SERCA-like</em>, <em>5-HT receptor</em>) and neuroplasticity further highlighted the profound neural impact of mating. Furthermore, a weighted gene co-expression network analysis (WGCNA) identified hub genes involved in cytoskeletal dynamics, protein folding, and vesicular transport that regulate ovarian development. Collectively, these findings propose a model in which mating activates a conserved neuroendocrine interface. This process reshapes neural physiology by modulating calcium signaling, neurotransmission, and neurodevelopment, ultimately driving ovarian development and adaptive reproductive behaviors in <em>S. paramamosain</em>.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"57 ","pages":"Article 101696"},"PeriodicalIF":2.2,"publicationDate":"2025-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145662981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated transcriptomic and proteomic insights into low-salinity stress adaptation in Penaeus monodon","authors":"Hongshan Diao ,&nbsp;Jianzhi Shi ,&nbsp;Song Jiang ,&nbsp;Qibin Yang ,&nbsp;Wenzhe Li ,&nbsp;Yundong Li ,&nbsp;Jianhua Huang ,&nbsp;Lishi Yang ,&nbsp;Yangyang Ding ,&nbsp;Falin Zhou","doi":"10.1016/j.cbd.2025.101699","DOIUrl":"10.1016/j.cbd.2025.101699","url":null,"abstract":"<div><div>Salinity is a critical environmental factor affecting the growth of crustaceans. As an economically important aquaculture species, the farming of <em>Penaeus monodon</em> is currently facing challenges from salinity fluctuations caused by climate change. However, studies utilizing multi-omics approaches to elucidate its molecular adaptation mechanisms to low salinity remain limited. This study systematically investigated the molecular regulatory mechanisms of <em>P. monodon</em> under low salinity stress (3 ‰) at different time points (6 h, 24 h, 96 h) using transcriptomic and proteomic technologies. A total of 927 DEGs and 928 DEPs were identified compared to the control group. This study revealed a dynamic adaptive strategy. At 6 h, <em>P. monodon</em> exhibited disruptions in energy metabolism and immune suppression, alongside the activation of immediate compensatory pathways. As the stress continued to 24 h, <em>P. monodon</em> showed a broad enhancement of metabolic activity, indicating a systemic effort to mitigate stress damage. After 96 h of exposure, <em>P. monodon</em> demonstrated a sustained upregulation of energy metabolism and the activation of detoxification systems, facilitating stable adaptive regulation. Furthermore, transcriptome-proteome integration analysis uncovered coordinated gene-protein regulatory patterns. This study provides the first multi-omics atlas of <em>P. monodon</em>'s response to low salinity, which delineates a time-resolved molecular adaptation strategy. Our findings not only offer novel insights into osmoregulation but also deliver valuable molecular targets for breeding stress-resistant strains, presenting scientific basis for sustainable aquaculture facing environmental challenges.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"58 ","pages":"Article 101699"},"PeriodicalIF":2.2,"publicationDate":"2025-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145776755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrative analysis of transcriptomics and gut microbiome in grass carp under alkalinity stress","authors":"Jianlin Guo ,&nbsp;Yi Xu ,&nbsp;Qi Chen ,&nbsp;Haiqi Zhang","doi":"10.1016/j.cbd.2025.101698","DOIUrl":"10.1016/j.cbd.2025.101698","url":null,"abstract":"<div><div>Alkalinity, a key environmental stressor in saline-alkali ecosystems, adversely affects the growth and survival of aquatic organisms. Genetic improvement of alkali-tolerant fish strains offers a promising strategy for utilizing saline-alkaline water resources; however, the molecular mechanisms underlying the response to alkalinity stress remain inadequately understood. This study aimed to identify novel molecular signatures of alkaline exposure in grass carp by integrating gut microbiome profiling with host transcriptome data. Histological analysis revealed significant alterations in the height of intestinal folds, muscle layer thickness, and fold width in response to NaHCO₃ exposure, along with an increased number of goblet cells under alkalinity stress. Differential gene expression (DEGs) analysis identified 1620, 6564, and 3190 genes with significant expression changes at 24, 48, and 72 h of NaHCO₃ treatment, respectively, compared to controls. Several known alkalinity-responsive genes, such as aquaporin 1a (<em>aqp1a</em>), carbonic anhydrase 6 (<em>ca6</em>), heat shock protein 30 (<em>hsp30</em>), prostaglandin-endoperoxide synthase 2b (<em>ptgs2b</em>), caspase 23 (<em>casp23</em>), solute carrier family 7a (<em>slc7a</em>), toll-like receptor 5 (<em>tlr5</em>), and toll-like receptor 13 (<em>tlr13</em>), were identified and validated through real-time quantitative PCR. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses revealed that immune- and disease-related signaling pathways played critical roles in mitigating acute alkaline stress. Furthermore, a total of 1,521,323 quality-filtered sequences with an average of 416 bp in length were generated through 16S rRNA sequencing. Bioinformatics analysis indicated that NaHCO₃ exposure reduced microbial diversity and altered the gut microbiota composition in grass carp. Notably, Fusobacteriota abundance significantly increased, while Firmicutes and Proteobacteria decreased substantially at 48 h post-alkalinity stress. Integrative analysis further highlighted strong correlations between specific bacterial taxa and alkalinity-responsive differentially expressed genes (DEGs). These findings provided valuable insights into the molecular mechanisms underlying alkalinity stress and identified potential targets for molecular breeding to enhance alkaline tolerance in grass carp.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"57 ","pages":"Article 101698"},"PeriodicalIF":2.2,"publicationDate":"2025-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145662899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Short-term and persistent changes to the muscle transcriptome of Lake sturgeon (Acipenser fulvescens) following early-life exposures to elevated temperatures","authors":"William S. Bugg ,&nbsp;Matt J. Thorstensen ,&nbsp;Alyssa M. Weinrauch ,&nbsp;Catherine Brandt ,&nbsp;Ken M. Jeffries ,&nbsp;W. Gary Anderson","doi":"10.1016/j.cbd.2025.101697","DOIUrl":"10.1016/j.cbd.2025.101697","url":null,"abstract":"<div><div>In fishes, environmental change during early development may impact performance later in life. Exposure to elevated temperatures during these periods may result in a variety of changes to organismal physiology including both short-term and persistent impacts to growth. However, the underlying mechanisms which promote these physiological changes are unclear. In this study, we used mRNA-seq of white muscle tissue to investigate mechanisms underlying short-term (3 vs. 6 months post-hatch) and persistent (3 vs. 13 months post-hatch) impacts of temperature (16 °C, 18 °C, and 20 °C) during early development, which underlie enhanced growth performance in young of year lake sturgeon. Functional analysis of differentially expressed transcripts revealed that in the short-term, elevated temperatures of 20 °C had impacts on transcriptional regulation of epigenetic mechanisms, neuron development, muscle structure and function, and energy supply. Further, persistent effects led to the establishment of increased growth, altered muscle phenotypes, and transcriptional changes to alternative splicing, neuron signaling, as well as muscle type and function. Taken together, these results suggest that at 20 °C, epigenetic modifications may lead to a molecular switch inducing neuromuscular junction proliferation, which in turn alters developmental trajectories by increasing lake sturgeon muscle development and growth. These findings are pertinent to hatchery management processes and the impacts of increasing temperatures in natural environments such as from heat waves during early development, which both may have persistent impacts on the developmental trajectory of fishes.</div></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"57 ","pages":"Article 101697"},"PeriodicalIF":2.2,"publicationDate":"2025-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145623605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}