Biopreservation and Biobanking最新文献

Background: Cryopreservation causes harmful effects on sperm quality due to reactive oxygen species (ROS) overproduction and physical-chemical modifications, resulting in reduced sperm fertility potential. Recently, many studies have shown that adding antioxidants to the cryopreservation medium can markedly reduce these damages. The present study aimed to evaluate the effects of pre-treatment with curcumin at 0, 20, 50, and 100 μM concentrations on frozen-thawed human sperm parameters. Methods: Semen samples from 25 normozoospermic men were collected. Then, each sample was divided into five equal parts: fresh group and frozen-thawed groups, including 0, 20, 50, and 100 μM of curcumin. Pre-cryopreservation and post-thaw sperm motility, morphology, vitality, DNA fragmentation, and ROS levels were investigated. Results: Cryopreservation significantly reduced sperm quality. A known value of 50 μM curcumin significantly improved sperm progressive motility (18.67 ± 1.12 vs. 11.2 ± 1.24, p < 0.01), vitality (35.50 ± 1.63 vs. 21.83 ± 2.64, p < 0.05), and decreased ROS levels (p < 0.05), 50 μM curcumin also efficiently preserved sperm morphology after thawing (13.55 ± 0.33 vs. 6.56 ± 0.16, p < 0.001). Furthermore, the application of 50 μM curcumin resulted in a reduction in DNA fragmentation, though it did not reach statistical significance (p = 0.08). In contrast, 20 μM curcumin only had a significant impact on progressive motility (15.85 ± 0.7 vs. 11.2 ± 1.24, p < 0.05), whereas, in the 100 μM group, there were no significant differences in any of the measured parameters compared with the control group. Conclusion: It seems that curcumin ameliorates cryopreservation-induced injury to sperm.

Introduction: Biobanks are a foundational infrastructure supporting research at scale and contributing to scientific progress. The increasing collection of samples and associated data presents challenges in terms of both physical and digital storage and handling. In North America and Europe health data protection frameworks have been in place for several years, regulating the use of collected personal data, including health care data, as those typically used by human biobanks. Yet, regulatory frameworks for biobanking, particularly in low- and middle-income settings, are highly fragmented, and little is known in this area. Objectives: This review focuses on identifying the health-related data protection frameworks in sub-Saharan African countries, as they are relevant to biobanking. Methods: We used complementary literature review approaches to ensure the completeness of our results for biobanking identified as "African," as well as for "disease-based," "country-based," and artificial intelligence-based approaches. Results: In total, 56 articles were identified and reviewed in full, 31 health-related acts and frameworks relevant to biobanking, and 24 general data protection acts and frameworks from 37 countries. In some countries, such as Kenya and Zambia, these acts were implemented, in some others, they were not. In most cases, as these regulatory frameworks have been recently created and implemented, there are little or no data relating to the impact of their implementation. Conclusion: Our findings confirm that regulatory frameworks for biobanking in sub-Saharan Africa are still in a consistent period of emergence, in an effort by national governments to address the existing fragmented landscape and support the development of research.

Southeast Asian countries are at the forefront of public health pressures due to a confluence of factors such as population growth, urbanization, environmental pollution, and infectious diseases (re)emergence. Therefore, the ability to be able to conduct research addressing local and regional needs is of paramount importance. As such, biobanking activities, the standardized collection of biological samples, and associated data, developed over the past few decades supporting ongoing biomedical and clinical research, as well as surveillance are of critical importance. However, the regulatory landscape of biobanking is not widely understood and reported, which this narrative review aims to address for the ASEAN member states. It is evident that there are specific regulatory arrangements within each ASEAN member state, which though may be sufficient for the current level of operations, are unlikely to support a regional sharing of biological samples, data, and eventually benefits from the conducted research. Additionally, legacy and often-overlapping regulatory frameworks exist, which raise the need of an eventual consolidation under a single framework. Thus, this field requires further study as well as the creation of viable, practical proposals that would allow for biobanking harmonization and thus the exchange of biological samples and data to be achieved regionally, if not further afield.

The purpose of this study, carried out in two experiments, was to investigate the antioxidant effect of Prosopis farcta in Experiment 1, and trehalose in Experiment 2 in the freezing extender, on the quality of frozen-thawed goat epididymal spermatozoa. Sperm samples were added to based egg-yolk Tris-extender containing experimental treatments. The first experimental treatments included the following: an extender of the control group without additive and extender containing 50, 100, or 150 µg/mL of Prosopis farcta ethanolic extract (PEE1, PEE2, and PEE3, respectively). Treatments of the second experiment include an extender of the control group without additive, an extender containing 100 mM of trehalose (Tr), an extender containing 100 µg/mL PEE2, and 100 µg/mL PEE2 + 100 mM Tr. The results of the first experiment showed that PEE2 compared with the control group led to a significant decrease (p < 0.05) in malondialdehyde (MDA) concentration. Also, PEE1 and PEE2 treatments resulted in a significant increase (p < 0.05) in motility parameters by computer-assisted sperm analysis, and MDA concentrations decreased significantly (p < 0.05) in all treatments compared with the control group. In general, the results of the present experiment showed that Prosopis farcta ethanolic extract at the level of 100 µg/mL was effective in improving the quality of frozen-thawed goat epididymal spermatozoa. Also, a combination of Prosopis farcta ethanolic extract and trehalose can be successful in freezing goat epididymal spermatozoa.

Introduction: The International Organization for Standardization (ISO) standard 20387:2018 outlines general requirements for biobanks to ensure competence, impartiality, and uniform operation. Reasons for seeking ISO 20387 accreditation are improved compliance, international recognition, and increased credibility of a biobank. Methods: The Philip Morris International (PMI) BioBank is an in-house biobank of an ISO/GxP-driven, for-profit organization. A cross-functional team analyzed ISO 20387 requirements and assessed them against the existing quality management system (QMS) to identify gaps and develop a remediation plan. This allowed the development of an implementation plan with clear timelines. An audit room was reserved for the auditor and PMI BioBank representatives for the two-day audit. In the second room, a group of internal experts were available to answer the auditor's questions, prepare solutions, and bring them to the audit room. Results: The gap analysis did not reveal any relevant deficits, given that the PMI BioBank follows the corporate QMS mainly based on ISO 17025. However, numerous checklist items require more specific adjustments to the existing QMS. Therefore, a corrective and preventive action (CAPA) was opened to demonstrate that gaps were identified and brought under control during the audit process. Four key areas required documentation employee competencies, complaint management, conduction of risk analysis, and impartiality maintenance. The audit lasted two days. One minor deviation was addressed with a detailed explanation of the process and was accepted by the auditor. Conclusions: Being part of an ISO/GxP-driven organization allowed the PMI BioBank to go through the ISO 20387 accreditation process rapidly. However, there was a noteworthy need for financial and manpower resources. Still, the benefits of demonstrating highly standardized processes are impactful as they enhance compliance and gain internal and external recognition, credibility, and reputation of an organization.

Introduction: Handling, freezing, and thawing of sperm causes oxidative stress, compromising sperm quality. Nanotechnology offers platforms for the smart delivery of antioxidants during these processes. Objectives: A solid lipid nanoparticle (SLN) was used to deliver linalool, as an antioxidant supplementation to Naval Medical Research Institute mouse sperm during handling, freezing, and thawing. Methods: Linalool-loaded solid lipid nanoparticle (L-SLN) was made using the self-assembly method. After the assessment of physicochemical properties, the impact of L-SLN (10, 20, 30, and 50 µg/mL) on sperm motility, viability, sperm DNA fragmentation (SDF), nitric oxide (NO) production, and the activity of superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase (CAT), was investigated after its addition to the handling, freezing, and thawing media. Results: L-SLN was successfully created with a size of 262 ± 9.5 and a zeta potential of -28.5 ± 7.12, with an extended-release over time. During handling and freezing, supplementing corresponding media with L-SLN resulted in increased sperm motility and viability, specifically at 30 µg/mL. The percentage of SDF also decreased in post-thawed sperm at 30 µg/mL. L-SLN also led to elevated post-thawed NO production at 20 µg/mL, as well as increased SOD activity at 20 and 30 µg/mL. It also enhanced CAT and GPx activity at 30 and 10 µg/mL respectively. In handling media, L-SLN at 10 µg/mL could enhance NO production, CAT, and SOD activity, and at 20 µg/mL also boosted NO production and GPx activity. Generally, there was no significant improvement in sperm parameters with the mutual concentration of L-SLN for thawing media. Conclusions: Treating sperm extender media with 20 and 30 µg/mL of L-SLN and handling media with 10 and 30 µg/mL of L-SLN could improve sperm parameters following these interventions. L-SLN is a new antioxidant for sperm handling and freezing media, which may be applicable in human reproductive efforts.

Biobanking is essential for advancing precision medicine. However, challenges persist in raising public awareness and addressing data protection concerns. This brief report outlines dissemination strategies by the Central Biobank Regensburg (Zentrale Biobank Regensburg-ZBR) and the BRoTHER (Biobank Research on Telemedical Approaches for Human Biobanks in a European Region) network to engage relevant target groups. In this context, five key recommendations are derived: (1) tailoring communication to audience needs, (2) using diverse outreach methods, (3) enhancing digital presence, (4) fostering cross-border collaboration, and (5) securing dedicated funding. These strategies aim to promote the social and scientific benefits of biobanking while ensuring its sustainability.