Biopreservation and Biobanking最新文献

Aim of the Survey: When it comes to collaboration between academic biobanks and the pharmaceutical/biotechnology industry, the criteria for effective collaborations are still unclear. Researchers in industry and academic biobanks can have different incentives and requirements that the other party is often not familiar with. This survey was conducted in an attempt to increase understanding of these fundamental knowledge gaps that may be obstacles to optimal collaboration between academia and industry. Key Findings from the Survey: There were 53 total respondents. Although this was a global survey, most respondents (n = 29) were from North America, likely reflecting overall investment in research in this region and possibly increased interactions between academia and industry as well. Most respondent academic biobanks collect multiple sample types with most (>90%) collecting both biofluids (including blood) and tissue. Most of the participating academic biobanks were aware that they were not (35%), or only partially (35%), using the full potential of their inventory. One option for increasing utilization rates is by collaborating with industry partners. The main issues when working with industry were perceived to be a combination of challenges including contractual (55%), consent restrictions (45%), timelines (41%), or time pressure (36%). Time taken to put agreements together was also a significant hurdle (54%), together with the industry's administrative requirements (36%). Brief Conclusions from the Survey: To take advantage of opportunities for joint collaboration, it is essential that the parties involved build trust. The first step is to understand the different requirements and needs of the other party and to establish efficient structures for joint cooperation. This survey has highlighted key areas to be addressed as the next steps for strengthening bonds between academic biobanks and industry partners.

Introduction: Rosemary shrub/plant and Sericin have been documented to show antioxdative properties, however their role in improving post thaw semen quality has not been well established. Objectives: The present study was conducted to investigate the effect of Rosemary leaves extract and Sericin protein on post-thaw quality of Pantja buck semen. Methods: In the first experiment, 32 ejaculates were collected from 4 sexually mature Pantja bucks and pooled to form 8 pooled samples. The pooled samples were evaluated for seminal attributes (sperm motility, viability, morphology, plasma membrane integrity, and acrosomal integrity), status of antioxidative enzymes (superoxide dismutase, catalase, glutathione peroxidase, and glutathione reductase) and lipid-peroxidation (malondialdehyde) of sperm plasma membrane before dilution. In the second experiment, pooled samples were divided into three equal aliquots as group-C (Control), group-R (Rosemary), and group-S (Sericin). Aliquots of group-C were diluted in glycerolated Egg Yolk Tris (EYT) extender, whereas aliquots of group-R and group-S were additionally supplemented with 4.0% v/v Rosemary leaves extract and 0.25% w/v Sericin, respectively, and again examined for the above parameters at the post-dilution, post-equilibration, and post-thawing stages of semen freezing. Results: Significant differences (p < 0.05) were observed in the values of seminal attributes, level of enzymatic antioxidants, and lipid per-oxidation between group C and R and between group C and S at the post-thaw stage of semen freezing. However Sericin was nonsignificantly better than Rosemary in improving post-thaw semen quality. Conclusion: The results of the present study on limited semen samples in Pantja buck demonstrated that compared to the control group, both Rosemary aqueous extract (4%) and Sericin protein (0.25%) as an additive in TRIS extender, showed better cryoprotective effects resulting in improved post-thaw seminal characteristics.

Nearly one-third of flora, fauna, and funga species on Earth are threatened with extinction. In response, the prevalence of repositories-often called "biobanks" or "genome resource banks"-for storing biological materials from threatened species has become more widespread. This research examined trends for the (1) terminology, (2) taxa representation, (3) global distribution, and (4) operational approach of biobanks versus genome resource banks relating to zoos and wildlife. Our literature search results indicate that although genome resource banking literature began earlier in the 1990s, biobanking has seen a surge in publications with over 3.5× more literature for biobanking since 2020. Genome resource bank articles were highly focused on mammals (68%), while biobanking literature focused more on multi-taxonomic overviews and less-studied taxa. Our search parameters found the largest number of wildlife biobanks in Europe (18) and the lowest number in South America (2), though results are likely impacted by the search being completed in English. Additionally, only 28% (7/25) of global biodiversity hotspots contain a wildlife biobank based on our methodology. While not all wildlife biobanking efforts are published or reported, these findings suggest that (1) "biobank" will likely be the more widely used term in the future, (2) more biobanking research is needed for non-mammalian taxa, (3) there are geographical gaps in wildlife biobanks, and (4) conservation biobanking programs should focus on storing biospecimens from a wide set of individuals and develop assisted reproductive technologies concomitantly with the goal of maintaining healthy, sustainable populations in the long term.

Introduction: Sperm cryopreservation is a useful storage technique in artificial insemination. Nanoparticles and nanovesicles such as exosomes are widely used in sperm cryopreservation procedures to alleviate cold-induced injury inflicted during sperm freezing. Objective: The objective of the present study was to examine the impact of varying concentrations of exosomes derived from seminal plasma added to a freezing extender on the quality of post-thawed bull sperm. Methods: Five Holstein bulls were chosen based on their samples having less than 30% progressive motility. After exosome extraction, semen samples from bulls (n = 5) with progressive sperm motility ≤30% were collected, diluted with different exosome concentrations (0, 25, 50, and 100 μg/mL), and aspirated into 0.5 mL straws. After the freeze-thaw process, sperm total and progressive motility, viability, morphology, plasma membrane integrity, mitochondrial activity, and apoptosis status were assessed. Furthermore, the expression levels of annexin (ANX1), dystrophy-associated Fer-1-like protein (DYSF), fibronectin 1 (FN1), and reactive oxygen species modulator 1 (ROMO1) were evaluated via real-time polymerase chain reaction (PCR). Results: Adding different concentrations of exosomes (25, 50, and 150 μg/mL) significantly increased the progressive motility, viability, and membrane integrity of sperm compared with the control group (p < 0.05). For the apoptosis index, treatment with 100 μg/mL exosomes significantly increased the percentage of live cells (p < 0.05), while the percentage of necrotic cells decreased significantly (p < 0.05) compared with 25 μg/mL exosome. The results of quantitative PCR showed that the expression levels of ANX1 were significantly (p < 0.05) upregulated at 50 μg/mL exosome, and the expression of ROMO1, FN1, and DYSF were downregulated upon treatment with different exosome concentrations. Conclusions: In conclusion, supplementing the freezing diluent with exosome-derived seminal plasma could preserve the quality parameters of the post-thaw semen of the bull with low freezeability and could be used as a helpful method for reproductive programs.

Adequate hypothermic storage of human mesenchymal stem cells (hMSCs) is of fundamental importance since they have been explored in several regenerative medicine initiatives. However, the actual clinical application of hMSCs necessitates hypothermic storage for long periods, a process that requires the use of non-toxic and efficient cryo-reagents capable of maintaining high viability and differentiating properties after thawing. Current cryopreservation methods are based on cryoprotectant agents (CPAs) containing dimethylsulphoxide (DMSO), which have been shown to be toxic for clinical applications. In this study, we describe a simple and effective trehalose (TRE)-based solution to cryo-store human umbilical cord-derived MSCs (UC-MSCs) in liquid nitrogen. Cells viability, identity, chromosomal stability, proliferative and migration capacity, and stress response were assessed after cryopreservation in TRE as CPA, testing different concentrations by itself or in combination with ethylene glycol (EG). Here we show that TRE-stored UC-MSCs provided lower cell recovery rates compared with DMSO-based solution, but maintained good functional properties, stability, and differentiating potential. The best cell recovery was obtained using 0.5 M TRE with 10% EG showing no differences in the osteogenic, adipogenic, and chondrogenic differentiation capacity. A second cycle of cryopreservation in this TRE-based solution had no additional impact on the viability and morphology, although slightly affected cell migration. Furthermore, the expression of the stress-related genes, HSPA1A, SOD2, TP53, BCL-2, and BAX, did not show a higher response in UC-MSCs cryopreserved in 0.5 M TRE + 10% EG compared with DMSO. Together these results, in addition to ascertained therapeutic properties of TRE, provide sufficient evidence to consider TRE-based medium as a low-cost and efficient solution for the storage of human UC-MSCs cells and potentially substitute DMSO-based cryo-reagents.

Introduction: Biobanks of specimens of human origin have accumulated millions of specimens. Their storage is costly, while many of them may not be useful and should be culled. Objectives: Our objective was to develop, pilot test, and evaluate a quantitative culling tool. Methods: We developed a culling tool based on a series of parameters with a quantitative score attributed to each. The parameters of the culling tool correspond to different aspects of the value of collections, such as the richness of the associated data, the types of samples, their conservation mode, and regulatory constraints. Results: The culling tool was adapted and independently applied by the Foundation for Innovative New Diagnostics and the Biological Resource Center of Institut Pasteur biobanks. The cumulative final score supported evidence-based and standardized decision-making. A "diagnostic" threshold could be established for the "diagnosis" of collections of low value. Conclusion: The culling tool is an algorithm developed to assess the value of legacy collections of biological resources of human origin and help establish culling plans. Biobanks can use this culling tool when they periodically assess the value of stored collections and need to decide or advise to cull them, and also when deciding whether to accept requests to host new collections previously stored elsewhere.

Sperm cryopreservation (SC) is an acceptable laboratory procedure for long-term storage of sperm. However, this procedure causes sperm damage. This review aimed to systematically investigate the effects of herbal-based antioxidants (HBAs) application on sperm parameters during SC. Following determination of the main keywords and searching strategy, various databases were searched systematically. Primary and secondary screenings were applied based on inclusion/exclusion criteria. Finally, 27 randomized controlled trial studies using 15 different HBAs and involving 557 normal and 130 abnormal semen samples were included for the meta-analysis. HBAs administration before or during the SC process improved total motility (mean difference [MD]: -6.31 [95% confidence interval [CI]: -10.27, -2.34], p < 0.05), viability (MD: -1.21 [95% CI: -1.52, -0.89], p < 0.001), morphology (MD: -1.72 [95% CI: -2.89, -0.54], p < 0.05), reactive oxygen species (H₂O₂) (MD: 7.58 [95% CI: 3.90, 11.26], p < 0.001), DNA integrity (MD: -13.21 [95% CI: -19.94, -6.49], p < 0.001), and sperm DNA fragmentation (SDF) (MD: 3.76 [95% CI: 2.38, 5.14], p < 0.001) after thawing in normal specimens. In abnormal semen samples, the HBAs improved viability (MD: -8.54 [95% CI: -11.18, -5.19], p < 0.001), progressive motility (MD: -4.55 [95% CI: -7.37, -1.73], p < 0.05) and ameliorated SDF (MD: 3.76 [95% CI: 1.74, 5.79], p < 0.001). Also, HBAs had no effects on total motility (MD: -0.08 [95% CI: -3.29, 3.14], p > 0.05), and morphology (MD: -0.31 [95% CI: -0.71, 0.09], p > 0.05). Application of HBAs in cryomedia before and during SC can improve sperm parameters (including viability, motility, and morphology), decrease oxidative stress and SDF levels.