Pub Date : 2023-10-09DOI: 10.17267/2675-021xevidence.2023.e5173
Angélica Trevisan De Nardi, Leony Morgana Galliano, Nórton Luís Oliveira, Daniel Umpierre
BACKGROUND: The quality of reporting in the abstract section of scientific articles is one of the important aspects of good communication of trials. OBJECTIVES: We investigated abstracts of randomized clinical trials (RCTs) in the physical activity field according to adherence to the Consolidated Standards of Reporting Trials (CONSORT) for Abstracts (primary outcome) and checked the recommendations of the selected journals regarding the contents and structure of the abstract. METHODS: This study is a descriptive, cross-sectional study of the Strengthening the Evidence in Exercise Sciences (SEES) Initiative. RCTs published in 9 exercise science journals and 2 general medicine journals during 2019 were eligible. Two researchers conducted study selection and, thereafter, assessment of the abstracts using a form comprising 16 items based on CONSORT for Abstracts. Also, extracted, in duplicate and independently, the journals’ recommendations for authors. RESULTS: 131 abstracts were eligible for evaluation. From items evaluated, those with the highest adherence were objectives or hypothesis (99%), conclusion (98%), and intervention (94%). The lowest reporting was observed in the number of participants analyzed (6%), allocation and randomization (1%), and funding (1%). Ten journals recommended the abstract structure, but only two mentioned the CONSORT for Abstracts. CONCLUSIONS: There is variable and suboptimal adherence to the CONSORT for Abstracts in trials in the physical activity field and poor recommendation of this instrument in journals selected. Therefore, we suggest editors, reviewers, and authors a greater adherence to guidelines, and to journal recommendations to improve the quality of reporting of abstracts in the physical activity field.
背景:科学论文摘要部分的报道质量是试验良好传播的重要方面之一。目的:我们根据摘要综合报告试验标准(CONSORT)(主要结局)调查了体育活动领域的随机临床试验(rct)的摘要，并检查了所选期刊关于摘要内容和结构的推荐。方法:本研究是一项关于加强运动科学证据(SEES)倡议的描述性横断面研究。2019年在9种运动科学期刊和2种普通医学期刊上发表的随机对照试验符合条件。两名研究人员进行了研究选择，然后使用包含16个项目的基于CONSORT的摘要评估表对摘要进行评估。此外，还摘录了两份独立的期刊对作者的推荐。结果:131篇摘要符合评价标准。从评估项目来看，坚持度最高的是目标或假设(99%)、结论(98%)和干预(94%)。报告最低的是分析的参与者数量(6%)、分配和随机化(1%)和资助(1%)。10种期刊推荐了摘要结构，但只有2种期刊提到了CONSORT for Abstracts。结论:在体育活动领域的试验中，对CONSORT摘要的依从性存在变数和次优性，所选期刊对该工具的推荐度较差。因此，我们建议编辑、审稿人和作者更严格地遵守指南和期刊建议，以提高体育活动领域摘要报告的质量。
{"title":"Quality of reporting in abstracts of clinical trials using physical activity interventions: a cross-sectional analysis using the CONSORT for Abstracts","authors":"Angélica Trevisan De Nardi, Leony Morgana Galliano, Nórton Luís Oliveira, Daniel Umpierre","doi":"10.17267/2675-021xevidence.2023.e5173","DOIUrl":"https://doi.org/10.17267/2675-021xevidence.2023.e5173","url":null,"abstract":"BACKGROUND: The quality of reporting in the abstract section of scientific articles is one of the important aspects of good communication of trials. OBJECTIVES: We investigated abstracts of randomized clinical trials (RCTs) in the physical activity field according to adherence to the Consolidated Standards of Reporting Trials (CONSORT) for Abstracts (primary outcome) and checked the recommendations of the selected journals regarding the contents and structure of the abstract. METHODS: This study is a descriptive, cross-sectional study of the Strengthening the Evidence in Exercise Sciences (SEES) Initiative. RCTs published in 9 exercise science journals and 2 general medicine journals during 2019 were eligible. Two researchers conducted study selection and, thereafter, assessment of the abstracts using a form comprising 16 items based on CONSORT for Abstracts. Also, extracted, in duplicate and independently, the journals’ recommendations for authors. RESULTS: 131 abstracts were eligible for evaluation. From items evaluated, those with the highest adherence were objectives or hypothesis (99%), conclusion (98%), and intervention (94%). The lowest reporting was observed in the number of participants analyzed (6%), allocation and randomization (1%), and funding (1%). Ten journals recommended the abstract structure, but only two mentioned the CONSORT for Abstracts. CONCLUSIONS: There is variable and suboptimal adherence to the CONSORT for Abstracts in trials in the physical activity field and poor recommendation of this instrument in journals selected. Therefore, we suggest editors, reviewers, and authors a greater adherence to guidelines, and to journal recommendations to improve the quality of reporting of abstracts in the physical activity field.","PeriodicalId":55996,"journal":{"name":"International Journal of Evidence-Based Healthcare","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135093936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-03DOI: 10.17267/2675-021xevidence.2023.e5107
Charles Phillipe de Lucena Alves, João de Deus Barreto Segundo, Marina Christ Franco, Rafael Ratto De Moraes, David Moher, Maximiliano Sérgio Cenci, Tatiana Pereira-Cenci, Anelise Fernandes Montagner
INTRODUCTION: The debate on scientific research and reporting integrity issues in Brazil is incipient. Literature suggests that a journalology training course could help to improve the competencies of the participants. OBJECTIVE: To evaluate the immediate impact of a journalology training course on perceived academic competencies, comprised of knowledge, attitudes, and skills. METHODS: The course was taught in 5 consecutive days to an online audience of individuals from the health sciences. A self-applied questionnaire was employed before and immediately after the course, which included initial and acquired perceived knowledge, attitudes, skills. The Wilcoxon non-parametric test for paired samples was used for analysis. RESULTS: A total of 45 individuals participated in the course, with a 53% response rate before and after. The number of participants in each course session ranged between 32 and 45. There was an improvement in perceived knowledge of: (1) writing review articles; (2) ethical aspects of research; (3) scientific authorship; (4) predatory practices; (5) publication bias and spin, and (6) researcher evaluation. There was no improvement in self-reported attitudes towards any item. There was an improvement in the perception of skills relating to: (1) writing a response letter and (2) writing an opinion as a reviewer. CONCLUSIONS: Overall, attendees who participated in the survey reported perceived improved knowledge and skills in some items but not in their attitudes. Therefore, the course appears to have been unable to modify perceived scientific reporting competencies.
{"title":"Perceived impact of a one-week journalology training course on scientific reporting competencies: prospective survey","authors":"Charles Phillipe de Lucena Alves, João de Deus Barreto Segundo, Marina Christ Franco, Rafael Ratto De Moraes, David Moher, Maximiliano Sérgio Cenci, Tatiana Pereira-Cenci, Anelise Fernandes Montagner","doi":"10.17267/2675-021xevidence.2023.e5107","DOIUrl":"https://doi.org/10.17267/2675-021xevidence.2023.e5107","url":null,"abstract":"INTRODUCTION: The debate on scientific research and reporting integrity issues in Brazil is incipient. Literature suggests that a journalology training course could help to improve the competencies of the participants. OBJECTIVE: To evaluate the immediate impact of a journalology training course on perceived academic competencies, comprised of knowledge, attitudes, and skills. METHODS: The course was taught in 5 consecutive days to an online audience of individuals from the health sciences. A self-applied questionnaire was employed before and immediately after the course, which included initial and acquired perceived knowledge, attitudes, skills. The Wilcoxon non-parametric test for paired samples was used for analysis. RESULTS: A total of 45 individuals participated in the course, with a 53% response rate before and after. The number of participants in each course session ranged between 32 and 45. There was an improvement in perceived knowledge of: (1) writing review articles; (2) ethical aspects of research; (3) scientific authorship; (4) predatory practices; (5) publication bias and spin, and (6) researcher evaluation. There was no improvement in self-reported attitudes towards any item. There was an improvement in the perception of skills relating to: (1) writing a response letter and (2) writing an opinion as a reviewer. CONCLUSIONS: Overall, attendees who participated in the survey reported perceived improved knowledge and skills in some items but not in their attitudes. Therefore, the course appears to have been unable to modify perceived scientific reporting competencies.","PeriodicalId":55996,"journal":{"name":"International Journal of Evidence-Based Healthcare","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135690007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-31DOI: 10.17267/2675-021xevidence.2023.e5236
Ricardo Maia Ferreira
INTRODUCTION: Whenever there has been a technological revolution, there have been advantages and disadvantages associated with it. Artificial intelligence is now going through this phase of uncertainty. There are groups that agree and embrace this new technology, and others that simply refuse to let it into our daily lives. OBJECTIVE: The purpose of this reflection will be to understand the advantages and disadvantages of the use of artificial intelligence in health and science.
{"title":"Artificial intelligence in health and science: an introspection","authors":"Ricardo Maia Ferreira","doi":"10.17267/2675-021xevidence.2023.e5236","DOIUrl":"https://doi.org/10.17267/2675-021xevidence.2023.e5236","url":null,"abstract":"INTRODUCTION: Whenever there has been a technological revolution, there have been advantages and disadvantages associated with it. Artificial intelligence is now going through this phase of uncertainty. There are groups that agree and embrace this new technology, and others that simply refuse to let it into our daily lives. OBJECTIVE: The purpose of this reflection will be to understand the advantages and disadvantages of the use of artificial intelligence in health and science.","PeriodicalId":55996,"journal":{"name":"International Journal of Evidence-Based Healthcare","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73702608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-31DOI: 10.17267/2675-021xevidence.2023.e4970
André Demambre Bacchi, Bruna Stievano Bacchi
INTRODUÇÃO: A Análise do Discurso tem demonstrado que a neutralidade é algo inexistente na língua. Cada escolha lexical não é aleatória e, dentro de cada escolha, há uma diversidade de possibilidades de intenções, colocações e interpretações. Cultura, hábitos, crenças e todo o conjunto de características que compõe uma sociedade influenciam a construção da língua. Em uma sociedade na qual a ciência e o método científico estejam sendo subestimados ou ignorados, enquanto práticas pseudocientíficas são valorizadas, é esperado que haja um reflexo disso na linguagem. OBJETIVO: Discutir o impacto da linguagem e das escolhas lexicais na aparência de legitimidade científica de práticas pseudocientíficas. METODOLOGIA: Neste estudo, utilizamos ferramentas da linguística associadas ao pensamento científico para identificar de que forma as pseudociências podem se beneficiar da linguagem para aumentar sua credibilidade, com foco no uso de afixos. RESULTADOS: Falantes de uma língua possuem intuições sobre as regras formativas e significados relacionados aos afixos, mesmo sem estudarem formalmente as construções linguísticas ou epistemologia. Nesse sentido, práticas pseudocientíficas podem se beneficiar da aparência de legitimidade científica conferida pela percepção etimológica popular de sufixos e prefixos comumente empregados para designar áreas ou subáreas de estudos acadêmico-científicos. CONCLUSÃO: As palavras e afixos utilizados na linguagem científica não possuem definições precisas e inequívocas, mas sim, estão sujeitos a interpretações variadas e conflitantes. Diante dessa vulnerabilidade, a linguagem, como reflexo da sociedade em que estamos inseridos, pode limitar nossa capacidade de tomada de decisões racionais em relação à saúde.
{"title":"A relação entre linguagem e práticas pseudocientíficas","authors":"André Demambre Bacchi, Bruna Stievano Bacchi","doi":"10.17267/2675-021xevidence.2023.e4970","DOIUrl":"https://doi.org/10.17267/2675-021xevidence.2023.e4970","url":null,"abstract":"INTRODUÇÃO: A Análise do Discurso tem demonstrado que a neutralidade é algo inexistente na língua. Cada escolha lexical não é aleatória e, dentro de cada escolha, há uma diversidade de possibilidades de intenções, colocações e interpretações. Cultura, hábitos, crenças e todo o conjunto de características que compõe uma sociedade influenciam a construção da língua. Em uma sociedade na qual a ciência e o método científico estejam sendo subestimados ou ignorados, enquanto práticas pseudocientíficas são valorizadas, é esperado que haja um reflexo disso na linguagem. OBJETIVO: Discutir o impacto da linguagem e das escolhas lexicais na aparência de legitimidade científica de práticas pseudocientíficas. METODOLOGIA: Neste estudo, utilizamos ferramentas da linguística associadas ao pensamento científico para identificar de que forma as pseudociências podem se beneficiar da linguagem para aumentar sua credibilidade, com foco no uso de afixos. RESULTADOS: Falantes de uma língua possuem intuições sobre as regras formativas e significados relacionados aos afixos, mesmo sem estudarem formalmente as construções linguísticas ou epistemologia. Nesse sentido, práticas pseudocientíficas podem se beneficiar da aparência de legitimidade científica conferida pela percepção etimológica popular de sufixos e prefixos comumente empregados para designar áreas ou subáreas de estudos acadêmico-científicos. CONCLUSÃO: As palavras e afixos utilizados na linguagem científica não possuem definições precisas e inequívocas, mas sim, estão sujeitos a interpretações variadas e conflitantes. Diante dessa vulnerabilidade, a linguagem, como reflexo da sociedade em que estamos inseridos, pode limitar nossa capacidade de tomada de decisões racionais em relação à saúde.","PeriodicalId":55996,"journal":{"name":"International Journal of Evidence-Based Healthcare","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83500360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-14DOI: 10.17267/2675-021xevidence.2023.e4060
L. D. Borges, H. H. Dias, E. Ferreira, Paulyenny Machado Alves, Beatriz Oliveira Silva, Kíllya de Paiva Santos, G. D. da Costa, T. R. Moreira, Daniel Souza Santos, R. M. Cotta
BACKGROUND: Diabetes Mellitus (DM) is currently considered a global epidemic, with alarming estimates for the coming years on all continents, with Chronic Kidney Disease (CKD) as one of its main consequences when a timely diagnosis is not made. OBJECTIVE: The objective of this study is to estimate the prevalence of DM among individuals diagnosed with CKD by means of a systematic review and meta-analysis. METHODS: A systematic review was carried out in the main free-access databases such as Pubmed (Medlaine), Lilacs, Scopus and Scielo. Two researchers selected the articles, extracted the data and evaluated the quality. The collected data were evaluated using a random effects model. RESULTS: Of 994 articles, 17 studies were included that looked at three continents. The group prevalence of DM among individuals with CKD (95% CI) was 29% (23-35%), with heterogeneity I2 = 99,86% and p = 0.00, which was not explained by meta-regression and subgroups. CONCLUSIONS: The present study confirmed the high prevalence of DM among individuals with CKD, especially among those with end-stage renal disease, demonstrating the need for early diagnosis and timely treatment of DM and new studies in this area, considering the social and economic impact of these diseases worldwide.
背景:糖尿病(DM)目前被认为是一种全球性流行病，在未来几年中，各大洲都有令人担忧的预测，慢性肾脏疾病(CKD)是未及时诊断的主要后果之一。目的:本研究的目的是通过系统回顾和荟萃分析来估计慢性肾病患者中糖尿病的患病率。方法:对Pubmed (Medlaine)、Lilacs、Scopus、Scielo等主要免费数据库进行系统评价。两位研究者选择文章，提取数据并评估质量。使用随机效应模型对收集的数据进行评估。结果:在994篇文章中，17篇研究涉及三个大洲。CKD患者中DM的群体患病率(95% CI)为29%(23-35%)，异质性I2 = 99,86%， p = 0.00，不能用meta回归和亚组解释。结论:本研究证实了慢性肾病患者中糖尿病的高患病率，特别是在终末期肾病患者中，这表明需要早期诊断和及时治疗糖尿病，并考虑到这些疾病在世界范围内的社会和经济影响，在这一领域进行新的研究。
{"title":"Prevalence of Diabetes Mellitus among individuals with chronic kidney disease: systematic review and meta-analysis","authors":"L. D. Borges, H. H. Dias, E. Ferreira, Paulyenny Machado Alves, Beatriz Oliveira Silva, Kíllya de Paiva Santos, G. D. da Costa, T. R. Moreira, Daniel Souza Santos, R. M. Cotta","doi":"10.17267/2675-021xevidence.2023.e4060","DOIUrl":"https://doi.org/10.17267/2675-021xevidence.2023.e4060","url":null,"abstract":"BACKGROUND: Diabetes Mellitus (DM) is currently considered a global epidemic, with alarming estimates for the coming years on all continents, with Chronic Kidney Disease (CKD) as one of its main consequences when a timely diagnosis is not made. OBJECTIVE: The objective of this study is to estimate the prevalence of DM among individuals diagnosed with CKD by means of a systematic review and meta-analysis. METHODS: A systematic review was carried out in the main free-access databases such as Pubmed (Medlaine), Lilacs, Scopus and Scielo. Two researchers selected the articles, extracted the data and evaluated the quality. The collected data were evaluated using a random effects model. RESULTS: Of 994 articles, 17 studies were included that looked at three continents. The group prevalence of DM among individuals with CKD (95% CI) was 29% (23-35%), with heterogeneity I2 = 99,86% and p = 0.00, which was not explained by meta-regression and subgroups. CONCLUSIONS: The present study confirmed the high prevalence of DM among individuals with CKD, especially among those with end-stage renal disease, demonstrating the need for early diagnosis and timely treatment of DM and new studies in this area, considering the social and economic impact of these diseases worldwide.","PeriodicalId":55996,"journal":{"name":"International Journal of Evidence-Based Healthcare","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82630890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-13DOI: 10.17267/2675-021xevidence.2023.e4105
Flávia Quadros Lima, A. M. Marchionni, A. Medrado
INTRODUCTION: The use of biomodulatory therapies in order to help tissue repair has been increasingly common in different areas of health. OBJECTIVE: This study aims to comparatively evaluate the effects of 660 nm laser photobiomodulation, ozone therapy, and ozonated oil on repair through histomorphometric analysis in skin wounds in rats. Forty Wistar rats will be divided into 4 groups of 10 animals each, Control Group (GC), Laser Group (LG), Ozone Gas Group (OGG), and Ozonated Oil Group (OOG). MATERIALS AND METHODS: Standard skin wounds will be made on the back of the animals, and the different experimental groups will be treated with the biomodulatory therapies described for three consecutive days. Five and ten days after surgery, five rats from each group will be euthanized. Skin fragments, including the wound area, will be removed for histological processing and subsequent staining of histological sections with Hematoxylin-eosin and Sirius red. Micrographs of the histological sections will be obtained and ten standard images will be captured for quantitative evaluation of the variables collagen area, number of blood vessels, and epithelium thickness. The variables infiltrate of polymorphonuclear and monomorphonuclear inflammatory cells, as well as edema, will be analyzed semiquantitatively. Statistical analysis of the study variables will be performed, with a significance level of p<0.05. CONCLUSION: It is expected to verify which of the biomodulatory therapies used can favor the resolution of tissue repair, in particular, by promoting collagen biosynthesis.
{"title":"Histomorphometric evaluation of skin wounds in rats submitted to biomodulatory therapies – research protocol","authors":"Flávia Quadros Lima, A. M. Marchionni, A. Medrado","doi":"10.17267/2675-021xevidence.2023.e4105","DOIUrl":"https://doi.org/10.17267/2675-021xevidence.2023.e4105","url":null,"abstract":"INTRODUCTION: The use of biomodulatory therapies in order to help tissue repair has been increasingly common in different areas of health. OBJECTIVE: This study aims to comparatively evaluate the effects of 660 nm laser photobiomodulation, ozone therapy, and ozonated oil on repair through histomorphometric analysis in skin wounds in rats. Forty Wistar rats will be divided into 4 groups of 10 animals each, Control Group (GC), Laser Group (LG), Ozone Gas Group (OGG), and Ozonated Oil Group (OOG). MATERIALS AND METHODS: Standard skin wounds will be made on the back of the animals, and the different experimental groups will be treated with the biomodulatory therapies described for three consecutive days. Five and ten days after surgery, five rats from each group will be euthanized. Skin fragments, including the wound area, will be removed for histological processing and subsequent staining of histological sections with Hematoxylin-eosin and Sirius red. Micrographs of the histological sections will be obtained and ten standard images will be captured for quantitative evaluation of the variables collagen area, number of blood vessels, and epithelium thickness. The variables infiltrate of polymorphonuclear and monomorphonuclear inflammatory cells, as well as edema, will be analyzed semiquantitatively. Statistical analysis of the study variables will be performed, with a significance level of p<0.05. CONCLUSION: It is expected to verify which of the biomodulatory therapies used can favor the resolution of tissue repair, in particular, by promoting collagen biosynthesis.","PeriodicalId":55996,"journal":{"name":"International Journal of Evidence-Based Healthcare","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77640855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-05DOI: 10.17267/2675-021xevidence.2022.e4736
Rachel Trinchão Schneiberg Kalid Ribeiro, Élissa da Silva Santos, R. E. Mascarenhas, M. W. Mancini, L. Almeida-Lopes, T. F. Barros, Carlos Gustavo Regis, Jacqueline de Jesus Silva, Diogo Rodrigo de Magalhães Moreira, Daniel Oliveira Dantas, Beatriz Trinchão Andrade, Jéssica Mirella de Souza Gomes, Cristiane Maria Carvalho Costa Dias, Maria Fernanda Rios Grassi
BACKGROUND: Candida spp and Malassezia spp cause superficial infections that may be resistant to conventional treatments. Violet light-emitting diodes (LEDs) therapy is a therapeutic alternative. PURPOSE: To describe the protocol for evaluating the antifungal effect of violet LEDs 410 nm ± 10 nm on Candida spp and Malassezia spp in vitro. PROTOCOL: LEDs 410 nm ± 10 nm are applied to a fungal suspension at fluences of 61.13 J/cm2, 91.70 J/cm2, and 183.39 J/cm2. The isolates are cultured for 48 to 72 hours. Colony forming units (CFUs) are quantified by visual counting and percent culture plate occupancy by digital analysis. Morphology is assessed by light microscopy and Gram staining, and yeast metabolism/function by transmission electron microscopy, assessment of reactive oxygen species, and DNA fragmentation. DATA ANALYSIS: the percentage of LEDs inhibition is calculated considering the growth of the negative control condition and the percentage of plate occupancy by yeasts by dividing the number of pixels classified as colonies by the total number of pixels on the plate. The morphological and functional aspects are described for the intervention and negative control. The ANOVA test is used to compare the mean percentages of growth inhibition and plate occupancy between the three fluences of LEDs 410 nm ± 10 nm and the negative control. ESTIMATED RESULTS: We intend to determine the antifungal effect of the different fluences of LEDs 410 nm ± 10 nm on Candida spp and Malassezia spp. The evaluation of other fungal species by this protocol should be investigated.
{"title":"Protocol for evaluating the in vitro effect of violet light-emitting diodes (LEDs) 410 nm ± 10 nm on yeast cultures","authors":"Rachel Trinchão Schneiberg Kalid Ribeiro, Élissa da Silva Santos, R. E. Mascarenhas, M. W. Mancini, L. Almeida-Lopes, T. F. Barros, Carlos Gustavo Regis, Jacqueline de Jesus Silva, Diogo Rodrigo de Magalhães Moreira, Daniel Oliveira Dantas, Beatriz Trinchão Andrade, Jéssica Mirella de Souza Gomes, Cristiane Maria Carvalho Costa Dias, Maria Fernanda Rios Grassi","doi":"10.17267/2675-021xevidence.2022.e4736","DOIUrl":"https://doi.org/10.17267/2675-021xevidence.2022.e4736","url":null,"abstract":"BACKGROUND: Candida spp and Malassezia spp cause superficial infections that may be resistant to conventional treatments. Violet light-emitting diodes (LEDs) therapy is a therapeutic alternative. PURPOSE: To describe the protocol for evaluating the antifungal effect of violet LEDs 410 nm ± 10 nm on Candida spp and Malassezia spp in vitro. PROTOCOL: LEDs 410 nm ± 10 nm are applied to a fungal suspension at fluences of 61.13 J/cm2, 91.70 J/cm2, and 183.39 J/cm2. The isolates are cultured for 48 to 72 hours. Colony forming units (CFUs) are quantified by visual counting and percent culture plate occupancy by digital analysis. Morphology is assessed by light microscopy and Gram staining, and yeast metabolism/function by transmission electron microscopy, assessment of reactive oxygen species, and DNA fragmentation. DATA ANALYSIS: the percentage of LEDs inhibition is calculated considering the growth of the negative control condition and the percentage of plate occupancy by yeasts by dividing the number of pixels classified as colonies by the total number of pixels on the plate. The morphological and functional aspects are described for the intervention and negative control. The ANOVA test is used to compare the mean percentages of growth inhibition and plate occupancy between the three fluences of LEDs 410 nm ± 10 nm and the negative control. ESTIMATED RESULTS: We intend to determine the antifungal effect of the different fluences of LEDs 410 nm ± 10 nm on Candida spp and Malassezia spp. The evaluation of other fungal species by this protocol should be investigated.","PeriodicalId":55996,"journal":{"name":"International Journal of Evidence-Based Healthcare","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75103302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-11-21DOI: 10.17267/2675-021xevidence.2022.e4605
S. Decker, Lucas Emanuel Marzzani, Pedro Rotta de Ferreira
Narrative/ introduction. In the last edition of the Surviving Sepsis Campaign guidelines - SSC guidelines - recommendations regarding the optimization of antibiotics pharmacokinetics and pharmacodynamics (PK/PD) were made. Among these, the use of extended infusion of beta-lactams (penicillins, cephalosporins and carbapenems), are proposed to improve clinical and microbiological outcomes. However, according to the authors, studies of the economic implications of extended infusion – cost-effectiveness studies – are needed for these recommendations. Caveats. Sepsis represents a huge economic burden around the world due to the need for hospital and ICU beds, qualified staff and therapies for the treatment of the pathology, whereas it is known that antibiotics are the mainstay therapy. The basic research question for cost-effectiveness studies is to understand the superiority of a new and more expensive intervention over the "standard" therapy and, then, the implications of this in a health system and time perspective. However, extended infusion of antibiotics has shown a benefit in clinical outcomes and its use can reduce direct costs since is less expensive than the standard approach, considering that smaller amounts of the antibiotic are needed to reach the same PK/PD and clinical effect. Moreover, additional costs to do an extended infusion would not be significant, and more effective therapy could also reduce the indirect economic burden on the health systems. Therefore, it is beyond the scope of cost-effectiveness analyses, and should be incorporated by health systems.
{"title":"Extended infusion of cephalosporins, penicillins and carbapenems: a cost-effective point-of-view and critical appraisal of Surviving Sepsis Campaign guidelines","authors":"S. Decker, Lucas Emanuel Marzzani, Pedro Rotta de Ferreira","doi":"10.17267/2675-021xevidence.2022.e4605","DOIUrl":"https://doi.org/10.17267/2675-021xevidence.2022.e4605","url":null,"abstract":"Narrative/ introduction. In the last edition of the Surviving Sepsis Campaign guidelines - SSC guidelines - recommendations regarding the optimization of antibiotics pharmacokinetics and pharmacodynamics (PK/PD) were made. Among these, the use of extended infusion of beta-lactams (penicillins, cephalosporins and carbapenems), are proposed to improve clinical and microbiological outcomes. However, according to the authors, studies of the economic implications of extended infusion – cost-effectiveness studies – are needed for these recommendations.\u0000Caveats. Sepsis represents a huge economic burden around the world due to the need for hospital and ICU beds, qualified staff and therapies for the treatment of the pathology, whereas it is known that antibiotics are the mainstay therapy. The basic research question for cost-effectiveness studies is to understand the superiority of a new and more expensive intervention over the \"standard\" therapy and, then, the implications of this in a health system and time perspective. However, extended infusion of antibiotics has shown a benefit in clinical outcomes and its use can reduce direct costs since is less expensive than the standard approach, considering that smaller amounts of the antibiotic are needed to reach the same PK/PD and clinical effect. Moreover, additional costs to do an extended infusion would not be significant, and more effective therapy could also reduce the indirect economic burden on the health systems. Therefore, it is beyond the scope of cost-effectiveness analyses, and should be incorporated by health systems.","PeriodicalId":55996,"journal":{"name":"International Journal of Evidence-Based Healthcare","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87934043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-11-16DOI: 10.17267/2675-021xevidence.2022.e4354
P. Driusso, F. J. J. Reis, Jéssica Cordeiro Rodrigues, L. Pasqualotto, Camila Chiazuto Catai, Lilian Rose Mascarenhas, Simone Botelho, Cristine Homsi Jorge
INTRODUÇÃO: A iniciativa Choosing Wisely visa promover a discussão entre os profissionais da saúde e pacientes, a fim de reduzir os cuidados de baixo valor que não oferecem nenhum benefício ou podem causar prejuízos para a saúde. OBJETIVO: Descrever o processo de desenvolvimento da lista brasileira Choosing Wisely de Fisioterapia na Saúde da Mulher/Assoalho Pélvico. MÉTODO: A pesquisa será realizada em três etapas. Na primeira etapa, fisioterapeutas com experiência em Saúde da Mulher/Assoalho Pélvico serão convidados para compor um painel de especialistas e responderão a um formulário. O método Delphi será utilizado para se obter uma lista de declarações baseadas em evidência. Na segunda etapa, os membros da equipe de pesquisa analisarão todas as declarações fornecidas pelo painel de especialistas. Na terceira etapa, uma amostra de fisioterapeutas votará nas declarações selecionadas anteriormente. As declarações com mais votos na pesquisa nacional serão submetidas à Choosing Wisely Brasil, e uma lista final de recomendações será publicada.
{"title":"Iniciativa Choosing Wisely Brasil para a Fisioterapia na Saúde da Mulher/Assoalho Pélvico: protocolo de estudo","authors":"P. Driusso, F. J. J. Reis, Jéssica Cordeiro Rodrigues, L. Pasqualotto, Camila Chiazuto Catai, Lilian Rose Mascarenhas, Simone Botelho, Cristine Homsi Jorge","doi":"10.17267/2675-021xevidence.2022.e4354","DOIUrl":"https://doi.org/10.17267/2675-021xevidence.2022.e4354","url":null,"abstract":"INTRODUÇÃO: A iniciativa Choosing Wisely visa promover a discussão entre os profissionais da saúde e pacientes, a fim de reduzir os cuidados de baixo valor que não oferecem nenhum benefício ou podem causar prejuízos para a saúde. OBJETIVO: Descrever o processo de desenvolvimento da lista brasileira Choosing Wisely de Fisioterapia na Saúde da Mulher/Assoalho Pélvico. MÉTODO: A pesquisa será realizada em três etapas. Na primeira etapa, fisioterapeutas com experiência em Saúde da Mulher/Assoalho Pélvico serão convidados para compor um painel de especialistas e responderão a um formulário. O método Delphi será utilizado para se obter uma lista de declarações baseadas em evidência. Na segunda etapa, os membros da equipe de pesquisa analisarão todas as declarações fornecidas pelo painel de especialistas. Na terceira etapa, uma amostra de fisioterapeutas votará nas declarações selecionadas anteriormente. As declarações com mais votos na pesquisa nacional serão submetidas à Choosing Wisely Brasil, e uma lista final de recomendações será publicada.","PeriodicalId":55996,"journal":{"name":"International Journal of Evidence-Based Healthcare","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84904696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-11-04DOI: 10.17267/2675-021xevidence.2022.e4722
R. Leite
BACKGROUND: Randomized clinical trials (RCT) in critical care mostly return negative results. The research community discusses strategies to improve RCTs design. METHODS: This paper presents a theoretical framework based on marginal utility to treat the problems of hypothesis generation and treatment effects valuation and presents recently published high-quality studies as instances where such a framework predicts irrelevant findings. RESULTS: Blindness to marginal utility, i.e., inobservance of the marginal utility of the proposed intervention, is common in critical care RCTs. CONCLUSION: Critical care RCTs are usually blind to marginal utility and are, therefore, prone to produce irrelevant findings.
{"title":"More is exponentially less: marginal utility in critical care research","authors":"R. Leite","doi":"10.17267/2675-021xevidence.2022.e4722","DOIUrl":"https://doi.org/10.17267/2675-021xevidence.2022.e4722","url":null,"abstract":"BACKGROUND: Randomized clinical trials (RCT) in critical care mostly return negative results. The research community discusses strategies to improve RCTs design. METHODS: This paper presents a theoretical framework based on marginal utility to treat the problems of hypothesis generation and treatment effects valuation and presents recently published high-quality studies as instances where such a framework predicts irrelevant findings. RESULTS: Blindness to marginal utility, i.e., inobservance of the marginal utility of the proposed intervention, is common in critical care RCTs. CONCLUSION: Critical care RCTs are usually blind to marginal utility and are, therefore, prone to produce irrelevant findings.","PeriodicalId":55996,"journal":{"name":"International Journal of Evidence-Based Healthcare","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72623117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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