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Mental Health Prescribers' Perceptions on the Use of Pharmacogenetic Testing in the Management of Depression in the Middle East and North Africa Region. 中东和北非地区心理健康处方医师对使用药物遗传学检测治疗抑郁症的看法
IF 1.9 4区 医学 Q3 Pharmacology, Toxicology and Pharmaceutics Pub Date : 2023-01-01 DOI: 10.2147/PGPM.S410240
Shimaa Aboelbaha, Monica Zolezzi, Oraib Abdallah, Yassin Eltorki

Objective: A wide variety of commercial pharmacogenetic (PGx) tools are available worldwide to guide treatment selection for depression based on individuals' genetic profiles. However, the use of genetic testing to inform psychiatric care has faced challenges due to the limited training and education for mental health clinicians. The aim of this study was to explore the knowledge, level of engagement, and perspectives on the use of PGx testing when making depression management decisions among practicing psychiatrists within the Middle East and North Africa (MENA) region.

Methods: This is a qualitative study using semi-structured interviews. Consenting psychiatrists were interviewed through an online platform (SkypeTM or Microsoft TeamsTM). Interviews were audio recorded, transcribed, and thematically analyzed with the assistance of NVivo® software.

Results: Eighteen interviews from 12 countries have been conducted. Analysis of the current interviews produced five major themes including: (1) Overall perceptions and attitudes; (2) Knowledge and awareness; (3) Education, training, and professional experience; (4) Facilitators and barriers; and (5) Ethical dilemmas. These themes support the notion that there is limited, mostly basic, education, knowledge, and training regarding genetic testing in the management of depression, although there is significant interest and willingness in the part of prescribers to adopt this strategy in their practice.

Conclusion: The findings of the study suggest that psychiatrists practicing in the MENA region appear to be interested in implementing PGx testing when managing people with depression. However, it is also important to recognize that this cannot be achieved unless more supporting strategies are implemented within their current health system environment.

目的:在世界范围内,有各种各样的商业药物遗传学(PGx)工具可以根据个体的遗传特征来指导抑郁症的治疗选择。然而,由于对心理健康临床医生的培训和教育有限,使用基因检测来告知精神病学护理面临挑战。本研究的目的是探讨中东和北非(MENA)地区执业精神科医生在制定抑郁症管理决策时使用PGx测试的知识,参与水平和观点。方法:采用半结构化访谈法进行定性研究。通过在线平台(SkypeTM或Microsoft TeamsTM)对同意的精神科医生进行访谈。访谈录音,转录,并在NVivo®软件的帮助下进行主题分析。结果:共进行了来自12个国家的18次访谈。对当前访谈的分析产生了五个主要主题,包括:(1)总体看法和态度;(2)知识和意识;(三)学历、培训和从业经历;(4)促进因素和障碍;(5)伦理困境。这些主题支持这样一种观点,即在抑郁症的管理中,关于基因检测的教育、知识和培训是有限的,主要是基础的,尽管处方者在实践中有很大的兴趣和意愿采用这种策略。结论:研究结果表明,在中东和北非地区执业的精神科医生似乎对在治疗抑郁症患者时实施PGx测试感兴趣。然而,同样重要的是要认识到,除非在目前的卫生系统环境中实施更多的支持性战略,否则无法实现这一目标。
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引用次数: 1
A Preliminary Study on the Correlation Between Age and Endometrial Receptivity. 年龄与子宫内膜容受性相关性的初步研究。
IF 1.9 4区 医学 Q3 Pharmacology, Toxicology and Pharmaceutics Pub Date : 2023-01-01 DOI: 10.2147/PGPM.S406257
Song Guo, Di Zhang, Shan Zhao, Huan Zhang, Yijuan Sun, Li Yan

Background: It is well established that female fertility declines with age, primarily because of loss of ovarian function. However, few studies have clarified the relationship between increasing age and endometrial receptivity. Here, we aimed to study the impact of age on endometrial receptivity, meanwhile, we examined the expression of endometrial mesenchymal stem cell (eMSC) surface markers (CD146 and PDGF-Rβ), essential for endometrial development and re-growth, in different age groups.

Methods: Participants were enrolled in this study between October 2020 and July 2021. All 31 patients were divided into three age groups; early (30-39 years old, n=10), intermediate (40-49 years old, n=12) and advanced (≥50 years old, n=9). We assessed localization and expression of CD146 and PDGF-Rβ by immunofluorescence and further analyzed selected endometrial receptivity markers (Homeobox A10 HOXA10, leukemia inhibitory factor LIF and osteopontin) and steroid hormone receptors by immunohistochemistry.

Results: There were no significant differences in expression of HOXA10 and OPN (p>0.05) among the three groups. However, we found a significant difference in LIF expression between the early and advanced age groups, with higher expression noted in the latter group (p=0.02). Similarly, estrogen receptor (ER) and progesterone receptor (PR) expression were significantly increased (p=0.01 and p=0.01, respectively) in the advanced age group compared with the early age group. There were no significant difference in CD146 and PDGF-Rβ expression among the three groups (p>0.05).

Conclusion: These results suggest that the age of the patient does not influence their endometrial receptivity. So, this study serves to increase our understanding of the impact of age and eMSCs on endometrial receptivity and expands the etiology of age-related infertility.

背景:众所周知,女性生育能力随着年龄的增长而下降,主要是因为卵巢功能的丧失。然而,很少有研究阐明年龄增长与子宫内膜容受性之间的关系。在此,我们旨在研究年龄对子宫内膜容受性的影响,同时,我们检测了不同年龄组子宫内膜间充质干细胞(eMSC)表面标志物(CD146和PDGF-Rβ)的表达,这是子宫内膜发育和再生所必需的。方法:参与者在2020年10月至2021年7月期间入组。31例患者分为3个年龄组;早期(30-39岁,n=10)、中期(40-49岁,n=12)和晚期(≥50岁,n=9)。我们通过免疫荧光检测CD146和PDGF-Rβ的定位和表达,并进一步通过免疫组织化学分析选定的子宫内膜容受性标志物(Homeobox A10 HOXA10、白血病抑制因子LIF和骨桥蛋白)和类固醇激素受体。结果:三组患者HOXA10、OPN的表达差异无统计学意义(p>0.05)。然而,我们发现早期和晚期年龄组之间LIF表达有显著差异,后者表达较高(p=0.02)。老年组雌激素受体(ER)和孕激素受体(PR)的表达也明显高于早期组(p=0.01和p=0.01)。三组间CD146、PDGF-Rβ表达差异无统计学意义(p>0.05)。结论:这些结果提示患者的年龄不影响其子宫内膜容受性。因此,本研究有助于增加我们对年龄和eMSCs对子宫内膜容受性的影响的理解,并扩展年龄相关性不孕的病因学。
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引用次数: 0
The Influence of Cytochrome P450 Polymorphisms on Pharmacokinetic Profiles and Treatment Outcomes Among Malaria Patients in Sub-Saharan Africa: A Systematic Review. 细胞色素P450多态性对撒哈拉以南非洲疟疾患者药代动力学特征和治疗结果的影响:一项系统综述
IF 1.9 4区 医学 Q3 Pharmacology, Toxicology and Pharmaceutics Pub Date : 2023-01-01 DOI: 10.2147/PGPM.S379945
Karol J Marwa, Anthony Kapesa, Erasmus Kamugisha, Göte Swedberg

Background: Sub-Saharan Africa (SSA) population is genetically diverse and heterogenous thus variability in drug response among individuals is predicted to be high. Cytochrome P450 (CYP450) polymorphisms is a major source of variability in drug response. This systematic review presents the influence of CYP450 single nucleotide polymorphisms (SNPs), particularly CYP3A4*1B, CYP2B6*6 and CYP3A5*3 on antimalarial drug plasma concentrations, efficacy and safety in SSA populations.

Methods: Searching for relevant studies was done through Google Scholar, Cochrane Central Register of controlled trials (CENTRAL), PubMed, Medline, LILACS, and EMBASE online data bases. The Preferred Reporting Items for Systematic Reviews and Meta-analyses (PRISMA) guidelines were used. Two independent reviewers extracted data from the studies.

Results: Thirteen studies reporting the influence of CYP450 SNPs on plasma concentrations, efficacy and safety were included in the final data synthesis. CYP3A4*1B, CYP3A5*5, CYP2B6*6 and CYP2C8*2 did not affect antimalarial drug plasma concentration significantly. There was no difference in treatment outcomes between malaria patients with variant alleles and those with wild type alleles.

Conclusion: This review reports lack of influence of CYP3A4*1B, CYP3A5*3, CYP2C8*3 and CYP2B6*6 SNPs on PK profiles, efficacy and safety in SSA among P. falciparum malaria patients.

背景:撒哈拉以南非洲(SSA)人口具有遗传多样性和异质性,因此预测个体之间药物反应的变异性很高。细胞色素P450 (CYP450)多态性是药物反应变异性的主要来源。本系统综述了CYP450单核苷酸多态性(snp),特别是CYP3A4*1B、CYP2B6*6和CYP3A5*3对SSA人群抗疟药物血药浓度、疗效和安全性的影响。方法:通过Google Scholar、Cochrane Central Register of controlled trials (Central)、PubMed、Medline、LILACS和EMBASE在线数据库检索相关研究。采用了系统评价和荟萃分析的首选报告项目(PRISMA)指南。两名独立的审稿人从研究中提取了数据。结果:13项报告CYP450 snp对血药浓度、疗效和安全性影响的研究纳入最终数据合成。CYP3A4*1B、CYP3A5*5、CYP2B6*6、CYP2C8*2对抗疟药血药浓度无显著影响。携带变异等位基因的疟疾患者和携带野生型等位基因的疟疾患者的治疗结果没有差异。结论:CYP3A4*1B、CYP3A5*3、CYP2C8*3和CYP2B6*6 snp对恶性疟原虫SSA患者PK谱、疗效和安全性的影响尚不明确。
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引用次数: 1
Discovery of Drug-Responsive Phenomic Alteration-Related Driver Genes in the Treatment of Coronary Heart Disease. 冠心病治疗中药物反应性表型改变相关驱动基因的发现
IF 1.9 4区 医学 Q3 Pharmacology, Toxicology and Pharmaceutics Pub Date : 2023-01-01 DOI: 10.2147/PGPM.S398522
Shuang Guan, Ya-Nan Yu, Bing Li, Hao Gu, Lin Chen, Nian Wang, Bo Wang, Xi Liu, Jun Liu, Zhong Wang
Background The Xueyu Zheng (XYZ) phenome is central to coronary heart disease (CHD), but efforts to detect genetic associations in the XYZ phenome have been disappointing. Methods The phenomic alteration-related genes (PARGs) for the XYZ phenome were screened using |ρ| > 0.4 and p < 0.05 after treatment with Danhong injection at day 14 and day 30. Then, the driver genes for the Protein-Protein Interaction (PPI) networks of the PARGs established using STRING 11.0 were detected using a personalized network control algorithm (PNC). Finally, the molecular correlations of the driver genes with the XYZ phenome were analyzed with the Gene Ontology (GO) biological processes and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways from a holistic viewpoint. Results A total of 525 and 309 PARGs in the XYZ phenome at day 14 and day 30 were identified. These genes were separately enriched in 48 and 35 pathways. Furthermore, five driver genes were detected. These genes were mainly correlated with endoplasmic reticulum stress-mediated apoptosis and autophagy regulation, which could suppress atherosclerosis progression. Conclusion Our study detected the drug-responsive PARGs of the XYZ phenome in CHD and provides an exemplary strategy to investigate the genetic associations among this common phenome and its component symptoms in patients with CHD. Trial Registration ClinicalTrials.gov, NCT01681316; registered on September 7, 2012.
背景:血凝征(XYZ)表型是冠心病的核心,但检测XYZ表型的遗传关联的努力令人失望。方法:丹红注射液治疗后第14天、第30天,采用|ρ| > 0.4、p < 0.05筛选XYZ表型组的表型改变相关基因(PARGs)。然后,使用个性化网络控制算法(PNC)检测使用STRING 11.0建立的PARGs蛋白-蛋白相互作用(PPI)网络的驱动基因。最后,利用基因本体(GO)生物学过程和京都基因与基因组百科全书(KEGG)途径从整体角度分析了驱动基因与XYZ现象组的分子相关性。结果:在第14天和第30天,XYZ表型中共鉴定出525个和309个PARGs。这些基因分别在48条和35条途径中富集。此外,还检测到5个驱动基因。这些基因主要与内质网应激介导的细胞凋亡和自噬调控相关,可抑制动脉粥样硬化的进展。结论:我们的研究在冠心病患者中检测到XYZ表型的药物反应性PARGs,并提供了一种典型的策略来研究这种常见表型与其组成症状之间的遗传关联。试验注册:ClinicalTrials.gov, NCT01681316;于2012年9月7日注册。
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引用次数: 0
Integrated Bioinformatics Analysis and Experimental Verification of Immune Cell Infiltration and the Related Core Genes in Ulcerative Colitis. 溃疡性结肠炎免疫细胞浸润及相关核心基因的综合生物信息学分析与实验验证。
IF 1.9 4区 医学 Q3 Pharmacology, Toxicology and Pharmaceutics Pub Date : 2023-01-01 DOI: 10.2147/PGPM.S406644
Danya Zhao, Danping Qin, Liming Yin, Qiang Yang

Background: Ulcerative colitis is a recurrent autoimmune disease. At present, the pathogenesis of UC is not completely clear. Hence, the etiology and underlying molecular mechanism need to be further investigated.

Methods: Three sets of microarray datasets were included from the Gene Expression Omnibus database. The differentially expressed genes in two sets of datasets were analyzed using the R software, and the core genes of UC were screened using machine learning. The sensitivity and specificity of the core genes were evaluated with the receiver operating characteristic curve in another microarray dataset. Subsequently, the CIBERSORT tool was used to analyze the relationship between UC and its core genes and immune cell infiltration. To verify the relationship between UC and core genes and the relationship between core genes and immune cell infiltration in vivo.

Results: A total of 36 DEGs were identified. AQP8, HMGCS2, and VNN1 were determined to be the core genes of UC. These genes had high sensitivity and specificity in receiver operating characteristic curve analysis. According to the analysis of immune cell infiltration, neutrophils, monocytes, and macrophages were positively correlated with UC. AQP8, HMGCS2, and VNN1 were also correlated with immune cell infiltration to varying degrees. In vivo experiments verified that the expressions of neutrophils, monocytes, and macrophages increased in the UC colon. Furthermore, the expressions of AQP8 and HMGCS2 decreased, whereas that of VNN1 increased. Azathioprine treatment improved all the indicators to different degrees.

Conclusion: AQP8, HMGCS2, and VNN1 are the core genes of UC and exhibit different degrees of correlation with immune cells. These genes are expected to become new therapeutic targets for UC. Moreover, the occurrence and development of UC are influenced by immune cell infiltration.

背景:溃疡性结肠炎是一种复发性自身免疫性疾病。目前,UC的发病机制尚不完全清楚。因此,病因和潜在的分子机制需要进一步研究。方法:从Gene Expression Omnibus数据库中获取三组微阵列数据集。使用R软件分析两组数据集中的差异表达基因,并使用机器学习筛选UC的核心基因。核心基因的敏感性和特异性用另一个微阵列数据集的接收器工作特征曲线进行评估。随后,利用CIBERSORT工具分析UC及其核心基因与免疫细胞浸润的关系。验证UC与核心基因的关系以及核心基因与体内免疫细胞浸润的关系。结果:共鉴定出36个deg。AQP8、HMGCS2和VNN1是UC的核心基因。这些基因在受试者工作特性曲线分析中具有较高的敏感性和特异性。免疫细胞浸润分析发现,中性粒细胞、单核细胞和巨噬细胞与UC呈正相关。AQP8、HMGCS2、VNN1也与免疫细胞浸润有不同程度的相关性。体内实验证实,UC结肠中中性粒细胞、单核细胞和巨噬细胞的表达增加。AQP8和HMGCS2表达降低,VNN1表达升高。硫唑嘌呤处理对各指标均有不同程度的改善。结论:AQP8、HMGCS2、VNN1是UC的核心基因,与免疫细胞有不同程度的相关性。这些基因有望成为UC的新的治疗靶点。此外,UC的发生发展受免疫细胞浸润的影响。
{"title":"Integrated Bioinformatics Analysis and Experimental Verification of Immune Cell Infiltration and the Related Core Genes in Ulcerative Colitis.","authors":"Danya Zhao,&nbsp;Danping Qin,&nbsp;Liming Yin,&nbsp;Qiang Yang","doi":"10.2147/PGPM.S406644","DOIUrl":"https://doi.org/10.2147/PGPM.S406644","url":null,"abstract":"<p><strong>Background: </strong>Ulcerative colitis is a recurrent autoimmune disease. At present, the pathogenesis of UC is not completely clear. Hence, the etiology and underlying molecular mechanism need to be further investigated.</p><p><strong>Methods: </strong>Three sets of microarray datasets were included from the Gene Expression Omnibus database. The differentially expressed genes in two sets of datasets were analyzed using the R software, and the core genes of UC were screened using machine learning. The sensitivity and specificity of the core genes were evaluated with the receiver operating characteristic curve in another microarray dataset. Subsequently, the CIBERSORT tool was used to analyze the relationship between UC and its core genes and immune cell infiltration. To verify the relationship between UC and core genes and the relationship between core genes and immune cell infiltration in vivo.</p><p><strong>Results: </strong>A total of 36 DEGs were identified. <i>AQP8, HMGCS2</i>, and <i>VNN1</i> were determined to be the core genes of UC. These genes had high sensitivity and specificity in receiver operating characteristic curve analysis. According to the analysis of immune cell infiltration, neutrophils, monocytes, and macrophages were positively correlated with UC. <i>AQP8, HMGCS2</i>, and <i>VNN1</i> were also correlated with immune cell infiltration to varying degrees. In vivo experiments verified that the expressions of neutrophils, monocytes, and macrophages increased in the UC colon. Furthermore, the expressions of <i>AQP8</i> and <i>HMGCS2</i> decreased, whereas that of <i>VNN1</i> increased. Azathioprine treatment improved all the indicators to different degrees.</p><p><strong>Conclusion: </strong><i>AQP8, HMGCS2</i>, and <i>VNN1</i> are the core genes of UC and exhibit different degrees of correlation with immune cells. These genes are expected to become new therapeutic targets for UC. Moreover, the occurrence and development of UC are influenced by immune cell infiltration.</p>","PeriodicalId":56015,"journal":{"name":"Pharmacogenomics & Personalized Medicine","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/b1/f7/pgpm-16-629.PMC10296601.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10113420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Pyroptosis and Inflammasome-Related Genes-NLRP3, NLRC4 and NLRP7 Polymorphisms Were Associated with Risk of Lung Cancer. 焦亡和炎性小体相关基因nlrp3、NLRC4和NLRP7多态性与肺癌风险相关
IF 1.9 4区 医学 Q3 Pharmacology, Toxicology and Pharmaceutics Pub Date : 2023-01-01 DOI: 10.2147/PGPM.S424326
Xin Jing, Yuhui Yun, Xiang Ji, Ende Yang, Pei Li

Background: Cancer development and tumor immune microenvironment remodeling are closely linked to pyroptosis and inflammasome activation. However, little information is available in single nucleotide polymorphisms (SNPs) in pyroptosis and inflammasome-related genes in patients with lung cancer. This study aims to evaluate the associations between pyroptosis-related gene (NLRP3, NLRC4, and NLRP7) polymorphisms and the risk of lung cancer.

Methods: The MassARRAY platform was used to genotype six SNPs of the NLRP3, NLRC4, and NLRP7 genes in 660 lung cancer cases and 660 controls.

Results: Individuals with rs35829419-A, rs385076-C, and rs775882-T alleles exhibited a higher risk of lung cancer (p < 0.01), while rs212704-T appears protective (p = 0.006). The rs35829419-AA, rs385076-TC/CC, and rs775882-CT/TT genotypes were associated with various degrees of elevated risk of lung cancer (p<0.02), whereas rs212704-TT was associated with a reduced risk of the disease (p=0.014). Genetic models analysis showed that rs35829419, rs385076, and rs775882 was associated with an increased risk of lung cancer, while rs212704 was related to a reduced risk in all three models (p < 0.05). The four SNPs remained significant in smoker and nonsmoker subgroups (p < 0.05). However, rs35829419 was correlated with risk of adenocarcinoma and small cell lung cancer, and rs212704 was only protective for squamous cell carcinoma. The rs385076 and rs775882 were associated with all three pathological types (p < 0.01).

Conclusion: Besides providing candidate markers for identification of high-risk populations and early prevention of the disease, our research also provided new insight into anti-tumor strategies targeting inflammasomes and pyroptosis.

背景:肿瘤的发展和肿瘤免疫微环境重塑与焦亡和炎性体活化密切相关。然而,关于肺癌患者焦亡和炎性小体相关基因的单核苷酸多态性(snp)的信息很少。本研究的目的是评估热作用相关基因(NLRP3、NLRC4和NLRP7)多态性与肺癌风险的关系。方法:利用MassARRAY平台对660例肺癌患者和660例对照者的NLRP3、NLRC4和NLRP7基因的6个snp进行基因分型。结果:携带rs35829419-A、rs385076-C和rs775882-T等位基因的个体患肺癌的风险较高(p < 0.01),而携带rs212704-T等位基因的个体具有保护作用(p = 0.006)。rs35829419-AA、rs385076-TC/CC和rs775882-CT/TT基因型与不同程度的肺癌风险升高相关(pp=0.014)。遗传模型分析显示,rs35829419、rs385076和rs775882与肺癌风险增加相关,而rs212704与肺癌风险降低相关(p < 0.05)。这4个snp在吸烟者和非吸烟者亚组中仍然显著(p < 0.05)。然而,rs35829419与腺癌和小细胞肺癌的风险相关,rs212704仅对鳞状细胞癌有保护作用。rs385076和rs775882与3种病理类型均相关(p < 0.01)。结论:我们的研究除了为高危人群的识别和疾病的早期预防提供候选标志物外,还为针对炎症小体和焦亡的抗肿瘤策略提供了新的见解。
{"title":"Pyroptosis and Inflammasome-Related Genes-<i>NLRP3, NLRC4</i> and <i>NLRP7</i> Polymorphisms Were Associated with Risk of Lung Cancer.","authors":"Xin Jing,&nbsp;Yuhui Yun,&nbsp;Xiang Ji,&nbsp;Ende Yang,&nbsp;Pei Li","doi":"10.2147/PGPM.S424326","DOIUrl":"https://doi.org/10.2147/PGPM.S424326","url":null,"abstract":"<p><strong>Background: </strong>Cancer development and tumor immune microenvironment remodeling are closely linked to pyroptosis and inflammasome activation. However, little information is available in single nucleotide polymorphisms (SNPs) in pyroptosis and inflammasome-related genes in patients with lung cancer. This study aims to evaluate the associations between pyroptosis-related gene (<i>NLRP3, NLRC4,</i> and <i>NLRP7</i>) polymorphisms and the risk of lung cancer.</p><p><strong>Methods: </strong>The MassARRAY platform was used to genotype six SNPs of the <i>NLRP3, NLRC4,</i> and <i>NLRP7</i> genes in 660 lung cancer cases and 660 controls.</p><p><strong>Results: </strong>Individuals with rs35829419-A, rs385076-C, and rs775882-T alleles exhibited a higher risk of lung cancer (<i>p</i> < 0.01), while rs212704-T appears protective (<i>p</i> = 0.006). The rs35829419-AA, rs385076-TC/CC, and rs775882-CT/TT genotypes were associated with various degrees of elevated risk of lung cancer (<i>p</i><0.02), whereas rs212704-TT was associated with a reduced risk of the disease (<i>p</i>=0.014). Genetic models analysis showed that rs35829419, rs385076, and rs775882 was associated with an increased risk of lung cancer, while rs212704 was related to a reduced risk in all three models (<i>p</i> < 0.05). The four SNPs remained significant in smoker and nonsmoker subgroups (<i>p</i> < 0.05). However, rs35829419 was correlated with risk of adenocarcinoma and small cell lung cancer, and rs212704 was only protective for squamous cell carcinoma. The rs385076 and rs775882 were associated with all three pathological types (<i>p</i> < 0.01).</p><p><strong>Conclusion: </strong>Besides providing candidate markers for identification of high-risk populations and early prevention of the disease, our research also provided new insight into anti-tumor strategies targeting inflammasomes and pyroptosis.</p>","PeriodicalId":56015,"journal":{"name":"Pharmacogenomics & Personalized Medicine","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/25/87/pgpm-16-795.PMC10464886.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10482816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
PUS1 May Be a Potential Prognostic Biomarker and Therapeutic Target for Hepatocellular Carcinoma. PUS1可能是肝细胞癌的潜在预后生物标志物和治疗靶点。
IF 1.9 4区 医学 Q3 Pharmacology, Toxicology and Pharmaceutics Pub Date : 2023-01-01 DOI: 10.2147/PGPM.S405621
Chenlu Lan, Xinlei Huang, Xiwen Liao, Xin Zhou, Kai Peng, Yongguang Wei, Chuangye Han, Tao Peng, Jianyao Wang, Guangzhi Zhu

Objective: The mechanisms of pseudouridine synthase (PUS) are not definite in hepatocellular carcinoma (HCC), the objective of this study is to investigate the effect of PUS genes in HCC.

Materials and methods: Differentially expressed and prognostic gene of PUS enzymes was identified based on The Cancer Genome Atlas (TCGA), International Cancer Genome Consortium (ICGC) and Gene Expression Profiling Interactive Analysis (GEPIA) databases. For the identified gene, pseudouridine synthase 1 (PUS1), was used for further research. The clinicopathological feature of PUS1 was analyzed by Student's t-test. Prognostic significance was explored by Kaplan-Meier (KM) analysis and Cox proportional hazards regression model. Receiver operating characteristic (ROC) curve was applied to appraise diagnostic and prognostic value. The Database for Annotation, Visualization, and Integrated Discovery (DAVID) and Gene Set Enrichment Analysis (GSEA) were implemented to explore mechanism of PUS1. A Guangxi cohort was applied to verify differential expression. In vitro cell experiments were implemented to investigate the influence for proliferation, reactive oxygen species (ROS) level, migration, and invasion of HCC cells after a knockdown of PUS1.

Results: PUS1 was significantly overexpressed in HCC tissues, and patients with high PUS1 were related to unpromising clinicopathological features. Survival analysis revealed high PUS1 expression was associated with a poor overall survival (OS) and 1 year-recurrence free survival (RFS), was an independent risk factor. Meanwhile, ROC curve showed that PUS1 had a diagnostic and prognostic significance to HCC. Functional enrichment analysis implied that PUS1 may be involved in metabolic pathways, mitochondrial function, non-alcoholic fatty liver disease (NAFLD), and some important carcinogenic pathways. Cell assays revealed that knockdown of PUS1 significantly constrained the migration, proliferation, invasion and improved the ROS level of HCC cells.

Conclusion: PUS1 may be a prognostic biomarker and a underlying treatment target for HCC.

目的:假尿嘧啶合酶(PUS)在肝细胞癌(HCC)中的作用机制尚不明确,本研究旨在探讨PUS基因在HCC中的作用。材料与方法:基于The Cancer Genome Atlas (TCGA)、International Cancer Genome Consortium (ICGC)和gene Expression Profiling Interactive Analysis (GEPIA)数据库,对PUS酶的差异表达及预后基因进行鉴定。对所鉴定的伪尿嘧啶合成酶1 (PUS1)基因进行进一步研究。采用Student’st检验分析PUS1的临床病理特征。采用Kaplan-Meier (KM)分析和Cox比例风险回归模型探讨预后意义。采用受试者工作特征(ROC)曲线评价诊断和预后价值。利用数据库注释、可视化和集成发现(DAVID)和基因集富集分析(GSEA)对PUS1的机制进行了研究。应用广西队列来验证差异表达。通过体外细胞实验研究了下调PUS1对肝癌细胞增殖、活性氧(ROS)水平、迁移和侵袭的影响。结果:PUS1在HCC组织中明显过表达,PUS1高表达的患者与临床病理特征不乐观相关。生存分析显示,PUS1高表达与较差的总生存期(OS)和1年无复发生存期(RFS)相关,是一个独立的危险因素。同时ROC曲线显示PUS1对HCC有诊断和预后意义。功能富集分析表明,PUS1可能参与代谢途径、线粒体功能、非酒精性脂肪性肝病(NAFLD)和一些重要的致癌途径。细胞实验显示,敲低PUS1可显著抑制HCC细胞的迁移、增殖、侵袭,提高ROS水平。结论:PUS1可能是HCC的预后生物标志物和潜在的治疗靶点。
{"title":"<i>PUS1</i> May Be a Potential Prognostic Biomarker and Therapeutic Target for Hepatocellular Carcinoma.","authors":"Chenlu Lan,&nbsp;Xinlei Huang,&nbsp;Xiwen Liao,&nbsp;Xin Zhou,&nbsp;Kai Peng,&nbsp;Yongguang Wei,&nbsp;Chuangye Han,&nbsp;Tao Peng,&nbsp;Jianyao Wang,&nbsp;Guangzhi Zhu","doi":"10.2147/PGPM.S405621","DOIUrl":"https://doi.org/10.2147/PGPM.S405621","url":null,"abstract":"<p><strong>Objective: </strong>The mechanisms of pseudouridine synthase (PUS) are not definite in hepatocellular carcinoma (HCC), the objective of this study is to investigate the effect of PUS genes in HCC.</p><p><strong>Materials and methods: </strong>Differentially expressed and prognostic gene of PUS enzymes was identified based on The Cancer Genome Atlas (TCGA), International Cancer Genome Consortium (ICGC) and Gene Expression Profiling Interactive Analysis (GEPIA) databases. For the identified gene, pseudouridine synthase 1 (<i>PUS1</i>), was used for further research. The clinicopathological feature of <i>PUS1</i> was analyzed by Student's <i>t</i>-test. Prognostic significance was explored by Kaplan-Meier (KM) analysis and Cox proportional hazards regression model. Receiver operating characteristic (ROC) curve was applied to appraise diagnostic and prognostic value. The Database for Annotation, Visualization, and Integrated Discovery (DAVID) and Gene Set Enrichment Analysis (GSEA) were implemented to explore mechanism of <i>PUS1</i>. A Guangxi cohort was applied to verify differential expression. In vitro cell experiments were implemented to investigate the influence for proliferation, reactive oxygen species (ROS) level, migration, and invasion of HCC cells after a knockdown of <i>PUS1</i>.</p><p><strong>Results: </strong><i>PUS1</i> was significantly overexpressed in HCC tissues, and patients with high <i>PUS1</i> were related to unpromising clinicopathological features. Survival analysis revealed high <i>PUS1</i> expression was associated with a poor overall survival (OS) and 1 year-recurrence free survival (RFS), was an independent risk factor. Meanwhile, ROC curve showed that <i>PUS1</i> had a diagnostic and prognostic significance to HCC. Functional enrichment analysis implied that <i>PUS1</i> may be involved in metabolic pathways, mitochondrial function, non-alcoholic fatty liver disease (NAFLD), and some important carcinogenic pathways. Cell assays revealed that knockdown of <i>PUS1</i> significantly constrained the migration, proliferation, invasion and improved the ROS level of HCC cells.</p><p><strong>Conclusion: </strong><i>PUS1</i> may be a prognostic biomarker and a underlying treatment target for HCC.</p>","PeriodicalId":56015,"journal":{"name":"Pharmacogenomics & Personalized Medicine","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/3d/65/pgpm-16-337.PMC10115212.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9758052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Expression and Correlation of MIF and ERK1/2 in Liver Cirrhosis and Hepatocellular Carcinoma Induced by Hepatitis B. MIF和ERK1/2在乙型肝炎诱导的肝硬化和肝细胞癌中的表达及相关性
IF 1.9 4区 医学 Q3 Pharmacology, Toxicology and Pharmaceutics Pub Date : 2023-01-01 DOI: 10.2147/PGPM.S398976
Dong Jia, Bin Li, Jun-Ke Wang, Pan Wang, Chu-Yi Li, Li-Xia Lu, Wen-Yan Tian, Xiao-Hui Yu, Jiu-Cong Zhang, Ying Zheng

Objective: To detect expression and phosphorylation level of macrophage migration inhibitor (MIF) and extracellular-regulated kinases 1 and 2 (ERK1/2) in hepatitis B-induced liver cirrhosis (HBILC) and hepatocellular carcinoma (HCC) with a background of HBILC and analyze the correlation of MIF and ERK1/2 with HBILC and HCC.

Methods: Twenty cases of normal liver tissues were collected as a control group, and 48 specimens of HBILC tissues and 48 specimens of HCC tissues were collected as the experimental group, which were assigned as the HBILC group and HCC group, respectively. All tissue specimens were processed into tissue chips. The expressions of MIF, ERK1/2, and their phosphorylated proteins were detected via immunohistochemistry, and MIF and ERK1/2 nucleic acid expressions were detected by in situ hybridization. The results were statistically analyzed using the chi-square test.

Results: Proteins and nucleic acids of MIF and ERK1/2 presented low expression in the control group and high expression in the HBILC group and HCC group. MIF expression in the three groups was 25.0%, 75.0%, and 79.17%, respectively, while that of the nucleic acids was 25.0%, 70.83%, and 68.75%, respectively. Expression of ERK1/2 in the three groups was 40.0%, 60.42%, and 81.25%, respectively, and that of nucleic acids was 40.0%, 79.17%, and 77.08%. Expression of pERK1/2 was low in the control and HBILC group and high in the HCC group. Expression of pERK1/2 in the three groups was 20%, 45.83%, and 75%, respectively. Expression of pERK1/2 in the HCC group was significantly different from that in the HBILC and control group (P<0.05), but the difference between the HBILC group and control group was not statistically significant (P>0.05).

Conclusion: Occurrence and development of HBILC and HCC are not only related to the high expression of MIF but also closely related to the activation of the ERK1/2 signaling pathway.

目的:检测巨噬细胞迁移抑制剂(MIF)和细胞外调节激酶1和2 (ERK1/2)在HBILC背景下乙型肝炎肝硬化(HBILC)和肝细胞癌(HCC)中的表达和磷酸化水平,分析MIF和ERK1/2与HBILC和HCC的相关性。方法:选取20例正常肝组织作为对照组,选取48例HBILC组织和48例HCC组织作为实验组,分别分为HBILC组和HCC组。所有组织标本均加工成组织芯片。免疫组化检测MIF、ERK1/2及其磷酸化蛋白表达,原位杂交检测MIF、ERK1/2核酸表达。结果采用卡方检验进行统计学分析。结果:MIF和ERK1/2蛋白及核酸在对照组呈低表达,在HBILC组和HCC组呈高表达。三组MIF表达量分别为25.0%、75.0%和79.17%,核酸表达量分别为25.0%、70.83%和68.75%。ERK1/2在三组中的表达量分别为40.0%、60.42%和81.25%,核酸的表达量分别为40.0%、79.17%和77.08%。pERK1/2在对照组和HBILC组低表达,在HCC组高表达。三组中pERK1/2的表达量分别为20%、45.83%和75%。pERK1/2在HCC组的表达与HBILC组及对照组比较差异有统计学意义(PP>0.05)。结论:HBILC和HCC的发生发展不仅与MIF的高表达有关,而且与ERK1/2信号通路的激活密切相关。
{"title":"Expression and Correlation of MIF and ERK1/2 in Liver Cirrhosis and Hepatocellular Carcinoma Induced by Hepatitis B.","authors":"Dong Jia,&nbsp;Bin Li,&nbsp;Jun-Ke Wang,&nbsp;Pan Wang,&nbsp;Chu-Yi Li,&nbsp;Li-Xia Lu,&nbsp;Wen-Yan Tian,&nbsp;Xiao-Hui Yu,&nbsp;Jiu-Cong Zhang,&nbsp;Ying Zheng","doi":"10.2147/PGPM.S398976","DOIUrl":"https://doi.org/10.2147/PGPM.S398976","url":null,"abstract":"<p><strong>Objective: </strong>To detect expression and phosphorylation level of macrophage migration inhibitor (MIF) and extracellular-regulated kinases 1 and 2 (ERK1/2) in hepatitis B-induced liver cirrhosis (HBILC) and hepatocellular carcinoma (HCC) with a background of HBILC and analyze the correlation of MIF and ERK1/2 with HBILC and HCC.</p><p><strong>Methods: </strong>Twenty cases of normal liver tissues were collected as a control group, and 48 specimens of HBILC tissues and 48 specimens of HCC tissues were collected as the experimental group, which were assigned as the HBILC group and HCC group, respectively. All tissue specimens were processed into tissue chips. The expressions of MIF, ERK1/2, and their phosphorylated proteins were detected via immunohistochemistry, and MIF and ERK1/2 nucleic acid expressions were detected by in situ hybridization. The results were statistically analyzed using the chi-square test.</p><p><strong>Results: </strong>Proteins and nucleic acids of MIF and ERK1/2 presented low expression in the control group and high expression in the HBILC group and HCC group. MIF expression in the three groups was 25.0%, 75.0%, and 79.17%, respectively, while that of the nucleic acids was 25.0%, 70.83%, and 68.75%, respectively. Expression of ERK1/2 in the three groups was 40.0%, 60.42%, and 81.25%, respectively, and that of nucleic acids was 40.0%, 79.17%, and 77.08%. Expression of pERK1/2 was low in the control and HBILC group and high in the HCC group. Expression of pERK1/2 in the three groups was 20%, 45.83%, and 75%, respectively. Expression of pERK1/2 in the HCC group was significantly different from that in the HBILC and control group (<i>P</i><0.05), but the difference between the HBILC group and control group was not statistically significant (<i>P</i>>0.05).</p><p><strong>Conclusion: </strong>Occurrence and development of HBILC and HCC are not only related to the high expression of MIF but also closely related to the activation of the ERK1/2 signaling pathway.</p>","PeriodicalId":56015,"journal":{"name":"Pharmacogenomics & Personalized Medicine","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/c5/c9/pgpm-16-381.PMC10145491.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9392985","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
NOTCH2, ATIC, MRI1, SLC6A13, ATP13A2 Genetic Variations are Associated with Ventricular Septal Defect in the Chinese Tibetan Population Through Whole-Exome Sequencing. NOTCH2、ATIC、MRI1、SLC6A13、ATP13A2基因变异与中国藏族人群室间隔缺损相关
IF 1.9 4区 医学 Q3 Pharmacology, Toxicology and Pharmaceutics Pub Date : 2023-01-01 DOI: 10.2147/PGPM.S404438
Xiaohui Zhang, Da Zhen, Xuemei Li, Faling Yi, Zhanhao Zhang, Wei Yang, Xuguang Li, Yemeng Sheng, Xiaoli Liu, Tianbo Jin, Yongjun He

Background: Ventricular septal defect (VSD) is the most common congenital cardiac abnormality in children and the second most common in adults. This study aimed to explore the potentially causative genes in VSD patients in the Chinese Tibetan population, and to provide a theoretical basis for the genetic mechanism of VSD.

Methods: Peripheral venous blood was collected from 20 VSD subjects, and whole-genome DNA was extracted. High-throughput sequencing was performed on qualified DNA samples using whole-exome sequencing (WES) technology. After filtering, detecting, and annotating qualified data, single nucleotide variations (SNVs) and insertion-deletion (InDel) markers were analyzed, and data processing software such as GATK, SIFT, Polyphen, and MutationTaster were used for comparative evaluation and prediction of pathogenic deleterious variants associated with VSD.

Results: A total of 4793 variant loci, including 4168 SNVs, 557 InDels and 68 unknown loci and 2566 variant genes were obtained from 20 VSD subjects through bioinformatics analysis. According to the screening of the prediction software and database, the occurrence of VSD was predicted to be associated with five inherited pathogenic gene mutations, all of which were missense mutations, including NOTCH2 (c.1396C >A:p.Gln466Lys), ATIC (c.235C >T:p.Arg79Cys), MRI1 (c.629G >A:p.Arg210Gln), SLC6A13 (c.1138G >A:p.Gly380Arg), ATP13A2 (c.1363C >T:p.Arg455Trp).

Conclusion: This study demonstrated that NOTCH2, ATIC, MRI1, SLC6A13, ATP13A2 gene variants were potentially associated with VSD in Chinese Tibetan population.

背景:室间隔缺损(VSD)是儿童最常见的先天性心脏畸形,其次是成人。本研究旨在探讨中国藏族人群VSD患者的潜在致病基因,为VSD的遗传机制提供理论依据。方法:采集20例VSD患者外周静脉血,提取全基因组DNA。采用全外显子组测序(WES)技术对合格的DNA样本进行高通量测序。在筛选、检测和注释合格数据后,分析单核苷酸变异(snv)和插入缺失(InDel)标记,并使用GATK、SIFT、Polyphen和MutationTaster等数据处理软件对与VSD相关的致病有害变异进行比较评估和预测。结果:通过生物信息学分析,从20例VSD受试者共获得4793个变异位点,其中snv 4168个,indel 557个,未知位点68个,变异基因2566个。通过预测软件和数据库的筛选,预测VSD的发生与5种遗传致病基因突变相关,均为错义突变,包括NOTCH2 (c.1396C >A:p.Gln466Lys)、ATIC (c.235C >T:p.Arg79Cys)、MRI1 (c.629G >A:p.Arg210Gln)、SLC6A13 (c.1138G >A:p.Gly380Arg)、ATP13A2 (c.1363C >T:p.Arg455Trp)。结论:NOTCH2、ATIC、MRI1、SLC6A13、ATP13A2基因变异与中国藏族人群VSD存在潜在关联。
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引用次数: 0
Leucyl and Cystinyl Aminopeptidase as a Prognostic-Related Biomarker in OV Correlating with Immune Infiltrates. 亮氨酸和胱氨酸氨基肽酶作为OV与免疫浸润相关的预后相关生物标志物。
IF 1.9 4区 医学 Q3 Pharmacology, Toxicology and Pharmaceutics Pub Date : 2023-01-01 DOI: 10.2147/PGPM.S400145
Qian Ma, Lei Chang, Wenwen Wang, Lingyi Che, Xiaoqin Song, Gailing Li, Ying Zhang, Yibing Chen, Zhuoyu Gu, Xin Ge

Background: It was indicated that tumor intrinsic heterogeneity and the tumor microenvironment (TME) of ovarian cancer (OV) influence immunotherapy efficacy and patient outcomes. Leucyl and cystinyl aminopeptidase (LNPEP) encodes a zinc-dependent aminopeptidase, which has been proved to participant in the vesicle-mediated transport and class I MHC mediated antigen processing and presentation. However, the function of LNPEP in TME of OV and its potential molecular mechanisms have not been determined. Therefore, we aimed to investigate a prognostic biomarker which may be helpful in identifying TME heterogeneity of ovarian cancer.

Methods: In this study, bioinformatics databases were used to explore the expression profile and immune infiltration of LNPEP. Bioinformatics analyses of survival data and interactors of LNPEP were conducted to predict the prognostic value of LNPEP in OV. The protein levels of LNPEP were validated by Western blot and immunohistochemistry.

Results: Based on the TCGA data, our data displayed that the mRNA expression of LNPEP was markedly down-regulated in ovarian cancer than that in para-cancer tissues, contrary to the protein level. Importantly, high LNPEP expression was associated with poor prognosis in patients with OV. Furthermore, Cox regression analysis showed that LNPEP was an independent prognostic factor in OV. GO and KEGG pathway analyses indicated the co-expressed genes of LNPEP were mainly related to a variety of immune-related pathways, including Th1 and Th2 cell differentiation, Th17 cell differentiation, and immunoregulatory interaction. Our data also demonstrated that the expression of LNPEP was strongly correlated with immune infiltration levels, immunomodulators, chemokines and chemokine receptors.

Conclusion: In our study, we identified and established a prognostic signature of immune-related LNPEP in OV, which will be of great value in predicting the prognosis of clinical trials and may become a new therapeutic target for immunological research and potential prognostic biomarker in OV.

背景:卵巢癌(OV)的肿瘤内在异质性和肿瘤微环境(TME)影响免疫治疗的疗效和患者预后。亮氨酸和胱氨酸氨基肽酶(LNPEP)编码锌依赖的氨基肽酶,该酶已被证明参与囊泡介导的转运和I类MHC介导的抗原加工和递呈。然而,LNPEP在OV TME中的作用及其潜在的分子机制尚未确定。因此,我们旨在研究一种可能有助于识别卵巢癌TME异质性的预后生物标志物。方法:利用生物信息学数据库对LNPEP的表达谱和免疫浸润进行研究。对LNPEP的生存数据和相互作用物进行生物信息学分析,以预测LNPEP在OV中的预后价值。Western blot和免疫组化检测LNPEP蛋白水平。结果:基于TCGA数据,我们的数据显示LNPEP mRNA在卵巢癌中的表达明显低于癌旁组织,与蛋白水平相反。重要的是,高LNPEP表达与OV患者的不良预后相关。此外,Cox回归分析显示LNPEP是OV的独立预后因素。GO和KEGG通路分析表明,LNPEP共表达基因主要与多种免疫相关通路相关,包括Th1和Th2细胞分化、Th17细胞分化、免疫调节相互作用等。我们的数据还表明,LNPEP的表达与免疫浸润水平、免疫调节剂、趋化因子和趋化因子受体密切相关。结论:本研究鉴定并建立了OV中免疫相关LNPEP的预后标志,对临床试验的预后预测具有重要价值,可能成为OV免疫学研究的新的治疗靶点和潜在的预后生物标志物。
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引用次数: 0
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Pharmacogenomics & Personalized Medicine
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