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Expression and Correlation of MIF and ERK1/2 in Liver Cirrhosis and Hepatocellular Carcinoma Induced by Hepatitis B. MIF和ERK1/2在乙型肝炎诱导的肝硬化和肝细胞癌中的表达及相关性
IF 1.9 4区 医学 Q3 PHARMACOLOGY & PHARMACY Pub Date : 2023-01-01 DOI: 10.2147/PGPM.S398976
Dong Jia, Bin Li, Jun-Ke Wang, Pan Wang, Chu-Yi Li, Li-Xia Lu, Wen-Yan Tian, Xiao-Hui Yu, Jiu-Cong Zhang, Ying Zheng

Objective: To detect expression and phosphorylation level of macrophage migration inhibitor (MIF) and extracellular-regulated kinases 1 and 2 (ERK1/2) in hepatitis B-induced liver cirrhosis (HBILC) and hepatocellular carcinoma (HCC) with a background of HBILC and analyze the correlation of MIF and ERK1/2 with HBILC and HCC.

Methods: Twenty cases of normal liver tissues were collected as a control group, and 48 specimens of HBILC tissues and 48 specimens of HCC tissues were collected as the experimental group, which were assigned as the HBILC group and HCC group, respectively. All tissue specimens were processed into tissue chips. The expressions of MIF, ERK1/2, and their phosphorylated proteins were detected via immunohistochemistry, and MIF and ERK1/2 nucleic acid expressions were detected by in situ hybridization. The results were statistically analyzed using the chi-square test.

Results: Proteins and nucleic acids of MIF and ERK1/2 presented low expression in the control group and high expression in the HBILC group and HCC group. MIF expression in the three groups was 25.0%, 75.0%, and 79.17%, respectively, while that of the nucleic acids was 25.0%, 70.83%, and 68.75%, respectively. Expression of ERK1/2 in the three groups was 40.0%, 60.42%, and 81.25%, respectively, and that of nucleic acids was 40.0%, 79.17%, and 77.08%. Expression of pERK1/2 was low in the control and HBILC group and high in the HCC group. Expression of pERK1/2 in the three groups was 20%, 45.83%, and 75%, respectively. Expression of pERK1/2 in the HCC group was significantly different from that in the HBILC and control group (P<0.05), but the difference between the HBILC group and control group was not statistically significant (P>0.05).

Conclusion: Occurrence and development of HBILC and HCC are not only related to the high expression of MIF but also closely related to the activation of the ERK1/2 signaling pathway.

目的:检测巨噬细胞迁移抑制剂(MIF)和细胞外调节激酶1和2 (ERK1/2)在HBILC背景下乙型肝炎肝硬化(HBILC)和肝细胞癌(HCC)中的表达和磷酸化水平,分析MIF和ERK1/2与HBILC和HCC的相关性。方法:选取20例正常肝组织作为对照组,选取48例HBILC组织和48例HCC组织作为实验组,分别分为HBILC组和HCC组。所有组织标本均加工成组织芯片。免疫组化检测MIF、ERK1/2及其磷酸化蛋白表达,原位杂交检测MIF、ERK1/2核酸表达。结果采用卡方检验进行统计学分析。结果:MIF和ERK1/2蛋白及核酸在对照组呈低表达,在HBILC组和HCC组呈高表达。三组MIF表达量分别为25.0%、75.0%和79.17%,核酸表达量分别为25.0%、70.83%和68.75%。ERK1/2在三组中的表达量分别为40.0%、60.42%和81.25%,核酸的表达量分别为40.0%、79.17%和77.08%。pERK1/2在对照组和HBILC组低表达,在HCC组高表达。三组中pERK1/2的表达量分别为20%、45.83%和75%。pERK1/2在HCC组的表达与HBILC组及对照组比较差异有统计学意义(PP>0.05)。结论:HBILC和HCC的发生发展不仅与MIF的高表达有关,而且与ERK1/2信号通路的激活密切相关。
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引用次数: 0
NOTCH2, ATIC, MRI1, SLC6A13, ATP13A2 Genetic Variations are Associated with Ventricular Septal Defect in the Chinese Tibetan Population Through Whole-Exome Sequencing. NOTCH2、ATIC、MRI1、SLC6A13、ATP13A2基因变异与中国藏族人群室间隔缺损相关
IF 1.9 4区 医学 Q3 PHARMACOLOGY & PHARMACY Pub Date : 2023-01-01 DOI: 10.2147/PGPM.S404438
Xiaohui Zhang, Da Zhen, Xuemei Li, Faling Yi, Zhanhao Zhang, Wei Yang, Xuguang Li, Yemeng Sheng, Xiaoli Liu, Tianbo Jin, Yongjun He

Background: Ventricular septal defect (VSD) is the most common congenital cardiac abnormality in children and the second most common in adults. This study aimed to explore the potentially causative genes in VSD patients in the Chinese Tibetan population, and to provide a theoretical basis for the genetic mechanism of VSD.

Methods: Peripheral venous blood was collected from 20 VSD subjects, and whole-genome DNA was extracted. High-throughput sequencing was performed on qualified DNA samples using whole-exome sequencing (WES) technology. After filtering, detecting, and annotating qualified data, single nucleotide variations (SNVs) and insertion-deletion (InDel) markers were analyzed, and data processing software such as GATK, SIFT, Polyphen, and MutationTaster were used for comparative evaluation and prediction of pathogenic deleterious variants associated with VSD.

Results: A total of 4793 variant loci, including 4168 SNVs, 557 InDels and 68 unknown loci and 2566 variant genes were obtained from 20 VSD subjects through bioinformatics analysis. According to the screening of the prediction software and database, the occurrence of VSD was predicted to be associated with five inherited pathogenic gene mutations, all of which were missense mutations, including NOTCH2 (c.1396C >A:p.Gln466Lys), ATIC (c.235C >T:p.Arg79Cys), MRI1 (c.629G >A:p.Arg210Gln), SLC6A13 (c.1138G >A:p.Gly380Arg), ATP13A2 (c.1363C >T:p.Arg455Trp).

Conclusion: This study demonstrated that NOTCH2, ATIC, MRI1, SLC6A13, ATP13A2 gene variants were potentially associated with VSD in Chinese Tibetan population.

背景:室间隔缺损(VSD)是儿童最常见的先天性心脏畸形,其次是成人。本研究旨在探讨中国藏族人群VSD患者的潜在致病基因,为VSD的遗传机制提供理论依据。方法:采集20例VSD患者外周静脉血,提取全基因组DNA。采用全外显子组测序(WES)技术对合格的DNA样本进行高通量测序。在筛选、检测和注释合格数据后,分析单核苷酸变异(snv)和插入缺失(InDel)标记,并使用GATK、SIFT、Polyphen和MutationTaster等数据处理软件对与VSD相关的致病有害变异进行比较评估和预测。结果:通过生物信息学分析,从20例VSD受试者共获得4793个变异位点,其中snv 4168个,indel 557个,未知位点68个,变异基因2566个。通过预测软件和数据库的筛选,预测VSD的发生与5种遗传致病基因突变相关,均为错义突变,包括NOTCH2 (c.1396C >A:p.Gln466Lys)、ATIC (c.235C >T:p.Arg79Cys)、MRI1 (c.629G >A:p.Arg210Gln)、SLC6A13 (c.1138G >A:p.Gly380Arg)、ATP13A2 (c.1363C >T:p.Arg455Trp)。结论:NOTCH2、ATIC、MRI1、SLC6A13、ATP13A2基因变异与中国藏族人群VSD存在潜在关联。
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引用次数: 0
Dysregulation of circRNA-0076906 and circRNA-0134944 is Correlated with Susceptibility to Osteoporosis and Osteoporotic Fracture in Postmenopausal Females from the Chinese Han Population. circRNA-0076906和circRNA-0134944基因异常与中国汉族绝经后女性骨质疏松和骨质疏松性骨折易感性相关
IF 1.9 4区 医学 Q3 PHARMACOLOGY & PHARMACY Pub Date : 2023-01-01 DOI: 10.2147/PGPM.S394757
Weijie Yang, Wei Zhang, Fengqian Li, Ning Xu, Ping Sun

Introduction: Many circRNAs, such as circRNA-0076906 and circRNA-0134944, have been reported to participate in the pathogenesis of osteoporosis via sponging miRNAs in postmenopausal female patients. In this study, we aimed to study potential signaling pathways underlying the role of certain circRNAs, miRNAs and their target genes in the pathogenesis of osteoporotic fracture in postmenopausal females.

Methods: Quantitative real-time PCR was performed to analyze the expression of circRNAs, miRNAs and their targets genes. Luciferase assays were carried out to explore the regulatory relationship between circ_0076906/miR-548i/OGN and circ_0134944/miR-630/TLR4.

Results: Osteoporosis and fracture were positively correlated to the expression of circ_0134944, miR-548i and TLR4, but negatively correlated to the expression of circ_0076906, miR-630 and OGN in the peripheral blood and bone tissue samples of postmenopausal women. Luciferase activities of wild-type circ_0076906 and OGN were inhibited by miR-548i, and the luciferase activities of wild-type circ_0134944 and TLR4 were suppressed by miR-630 in MG-63 and U-2 OS cells. Inhibition of circ_0076906 expression in MG-63 and U-2 OS cells activated the expression of miR-548i and inhibited the expression of OGN. Moreover, the overexpression of circ_0134944 in MG-63 and U-2 OS cells suppressed the expression of miR-630 and enhanced the expression of TLR4.

Conclusion: This study implied that the dysregulation of circRNA-0076906 and circRNA-0134944 modulated their specific signaling and thus contributed to the severity of osteoporosis, increasing the risk of osteoporotic fracture.

许多circrna,如circRNA-0076906和circRNA-0134944,已被报道通过海绵mirna参与绝经后女性患者骨质疏松的发病机制。在这项研究中,我们旨在研究某些circRNAs、miRNAs及其靶基因在绝经后女性骨质疏松性骨折发病机制中的潜在信号通路。方法:采用实时荧光定量PCR方法分析circrna、mirna及其靶基因的表达情况。通过荧光素酶测定来探索circ_0076906/miR-548i/OGN与circ_0134944/miR-630/TLR4之间的调控关系。结果:绝经后妇女外周血和骨组织样本中,骨质疏松和骨折与circ_0134944、miR-548i、TLR4的表达呈正相关,与circ_0076906、miR-630、OGN的表达呈负相关。在MG-63和U-2 OS细胞中,miR-548i抑制野生型circ_0076906和OGN的荧光素酶活性,miR-630抑制野生型circ_0134944和TLR4的荧光素酶活性。抑制MG-63和U-2 OS细胞中circ_0076906的表达可激活miR-548i的表达,抑制OGN的表达。此外,circ_0134944在MG-63和U-2 OS细胞中的过表达抑制了miR-630的表达,增强了TLR4的表达。结论:本研究提示circRNA-0076906和circRNA-0134944的异常调节了它们的特异性信号,从而导致了骨质疏松的严重程度,增加了骨质疏松性骨折的风险。
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引用次数: 0
PUS1 May Be a Potential Prognostic Biomarker and Therapeutic Target for Hepatocellular Carcinoma. PUS1可能是肝细胞癌的潜在预后生物标志物和治疗靶点。
IF 1.9 4区 医学 Q3 PHARMACOLOGY & PHARMACY Pub Date : 2023-01-01 DOI: 10.2147/PGPM.S405621
Chenlu Lan, Xinlei Huang, Xiwen Liao, Xin Zhou, Kai Peng, Yongguang Wei, Chuangye Han, Tao Peng, Jianyao Wang, Guangzhi Zhu

Objective: The mechanisms of pseudouridine synthase (PUS) are not definite in hepatocellular carcinoma (HCC), the objective of this study is to investigate the effect of PUS genes in HCC.

Materials and methods: Differentially expressed and prognostic gene of PUS enzymes was identified based on The Cancer Genome Atlas (TCGA), International Cancer Genome Consortium (ICGC) and Gene Expression Profiling Interactive Analysis (GEPIA) databases. For the identified gene, pseudouridine synthase 1 (PUS1), was used for further research. The clinicopathological feature of PUS1 was analyzed by Student's t-test. Prognostic significance was explored by Kaplan-Meier (KM) analysis and Cox proportional hazards regression model. Receiver operating characteristic (ROC) curve was applied to appraise diagnostic and prognostic value. The Database for Annotation, Visualization, and Integrated Discovery (DAVID) and Gene Set Enrichment Analysis (GSEA) were implemented to explore mechanism of PUS1. A Guangxi cohort was applied to verify differential expression. In vitro cell experiments were implemented to investigate the influence for proliferation, reactive oxygen species (ROS) level, migration, and invasion of HCC cells after a knockdown of PUS1.

Results: PUS1 was significantly overexpressed in HCC tissues, and patients with high PUS1 were related to unpromising clinicopathological features. Survival analysis revealed high PUS1 expression was associated with a poor overall survival (OS) and 1 year-recurrence free survival (RFS), was an independent risk factor. Meanwhile, ROC curve showed that PUS1 had a diagnostic and prognostic significance to HCC. Functional enrichment analysis implied that PUS1 may be involved in metabolic pathways, mitochondrial function, non-alcoholic fatty liver disease (NAFLD), and some important carcinogenic pathways. Cell assays revealed that knockdown of PUS1 significantly constrained the migration, proliferation, invasion and improved the ROS level of HCC cells.

Conclusion: PUS1 may be a prognostic biomarker and a underlying treatment target for HCC.

目的:假尿嘧啶合酶(PUS)在肝细胞癌(HCC)中的作用机制尚不明确,本研究旨在探讨PUS基因在HCC中的作用。材料与方法:基于The Cancer Genome Atlas (TCGA)、International Cancer Genome Consortium (ICGC)和gene Expression Profiling Interactive Analysis (GEPIA)数据库,对PUS酶的差异表达及预后基因进行鉴定。对所鉴定的伪尿嘧啶合成酶1 (PUS1)基因进行进一步研究。采用Student’st检验分析PUS1的临床病理特征。采用Kaplan-Meier (KM)分析和Cox比例风险回归模型探讨预后意义。采用受试者工作特征(ROC)曲线评价诊断和预后价值。利用数据库注释、可视化和集成发现(DAVID)和基因集富集分析(GSEA)对PUS1的机制进行了研究。应用广西队列来验证差异表达。通过体外细胞实验研究了下调PUS1对肝癌细胞增殖、活性氧(ROS)水平、迁移和侵袭的影响。结果:PUS1在HCC组织中明显过表达,PUS1高表达的患者与临床病理特征不乐观相关。生存分析显示,PUS1高表达与较差的总生存期(OS)和1年无复发生存期(RFS)相关,是一个独立的危险因素。同时ROC曲线显示PUS1对HCC有诊断和预后意义。功能富集分析表明,PUS1可能参与代谢途径、线粒体功能、非酒精性脂肪性肝病(NAFLD)和一些重要的致癌途径。细胞实验显示,敲低PUS1可显著抑制HCC细胞的迁移、增殖、侵袭,提高ROS水平。结论:PUS1可能是HCC的预后生物标志物和潜在的治疗靶点。
{"title":"<i>PUS1</i> May Be a Potential Prognostic Biomarker and Therapeutic Target for Hepatocellular Carcinoma.","authors":"Chenlu Lan,&nbsp;Xinlei Huang,&nbsp;Xiwen Liao,&nbsp;Xin Zhou,&nbsp;Kai Peng,&nbsp;Yongguang Wei,&nbsp;Chuangye Han,&nbsp;Tao Peng,&nbsp;Jianyao Wang,&nbsp;Guangzhi Zhu","doi":"10.2147/PGPM.S405621","DOIUrl":"https://doi.org/10.2147/PGPM.S405621","url":null,"abstract":"<p><strong>Objective: </strong>The mechanisms of pseudouridine synthase (PUS) are not definite in hepatocellular carcinoma (HCC), the objective of this study is to investigate the effect of PUS genes in HCC.</p><p><strong>Materials and methods: </strong>Differentially expressed and prognostic gene of PUS enzymes was identified based on The Cancer Genome Atlas (TCGA), International Cancer Genome Consortium (ICGC) and Gene Expression Profiling Interactive Analysis (GEPIA) databases. For the identified gene, pseudouridine synthase 1 (<i>PUS1</i>), was used for further research. The clinicopathological feature of <i>PUS1</i> was analyzed by Student's <i>t</i>-test. Prognostic significance was explored by Kaplan-Meier (KM) analysis and Cox proportional hazards regression model. Receiver operating characteristic (ROC) curve was applied to appraise diagnostic and prognostic value. The Database for Annotation, Visualization, and Integrated Discovery (DAVID) and Gene Set Enrichment Analysis (GSEA) were implemented to explore mechanism of <i>PUS1</i>. A Guangxi cohort was applied to verify differential expression. In vitro cell experiments were implemented to investigate the influence for proliferation, reactive oxygen species (ROS) level, migration, and invasion of HCC cells after a knockdown of <i>PUS1</i>.</p><p><strong>Results: </strong><i>PUS1</i> was significantly overexpressed in HCC tissues, and patients with high <i>PUS1</i> were related to unpromising clinicopathological features. Survival analysis revealed high <i>PUS1</i> expression was associated with a poor overall survival (OS) and 1 year-recurrence free survival (RFS), was an independent risk factor. Meanwhile, ROC curve showed that <i>PUS1</i> had a diagnostic and prognostic significance to HCC. Functional enrichment analysis implied that <i>PUS1</i> may be involved in metabolic pathways, mitochondrial function, non-alcoholic fatty liver disease (NAFLD), and some important carcinogenic pathways. Cell assays revealed that knockdown of <i>PUS1</i> significantly constrained the migration, proliferation, invasion and improved the ROS level of HCC cells.</p><p><strong>Conclusion: </strong><i>PUS1</i> may be a prognostic biomarker and a underlying treatment target for HCC.</p>","PeriodicalId":56015,"journal":{"name":"Pharmacogenomics & Personalized Medicine","volume":"16 ","pages":"337-355"},"PeriodicalIF":1.9,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/3d/65/pgpm-16-337.PMC10115212.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9758052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Leucyl and Cystinyl Aminopeptidase as a Prognostic-Related Biomarker in OV Correlating with Immune Infiltrates. 亮氨酸和胱氨酸氨基肽酶作为OV与免疫浸润相关的预后相关生物标志物。
IF 1.9 4区 医学 Q3 PHARMACOLOGY & PHARMACY Pub Date : 2023-01-01 DOI: 10.2147/PGPM.S400145
Qian Ma, Lei Chang, Wenwen Wang, Lingyi Che, Xiaoqin Song, Gailing Li, Ying Zhang, Yibing Chen, Zhuoyu Gu, Xin Ge

Background: It was indicated that tumor intrinsic heterogeneity and the tumor microenvironment (TME) of ovarian cancer (OV) influence immunotherapy efficacy and patient outcomes. Leucyl and cystinyl aminopeptidase (LNPEP) encodes a zinc-dependent aminopeptidase, which has been proved to participant in the vesicle-mediated transport and class I MHC mediated antigen processing and presentation. However, the function of LNPEP in TME of OV and its potential molecular mechanisms have not been determined. Therefore, we aimed to investigate a prognostic biomarker which may be helpful in identifying TME heterogeneity of ovarian cancer.

Methods: In this study, bioinformatics databases were used to explore the expression profile and immune infiltration of LNPEP. Bioinformatics analyses of survival data and interactors of LNPEP were conducted to predict the prognostic value of LNPEP in OV. The protein levels of LNPEP were validated by Western blot and immunohistochemistry.

Results: Based on the TCGA data, our data displayed that the mRNA expression of LNPEP was markedly down-regulated in ovarian cancer than that in para-cancer tissues, contrary to the protein level. Importantly, high LNPEP expression was associated with poor prognosis in patients with OV. Furthermore, Cox regression analysis showed that LNPEP was an independent prognostic factor in OV. GO and KEGG pathway analyses indicated the co-expressed genes of LNPEP were mainly related to a variety of immune-related pathways, including Th1 and Th2 cell differentiation, Th17 cell differentiation, and immunoregulatory interaction. Our data also demonstrated that the expression of LNPEP was strongly correlated with immune infiltration levels, immunomodulators, chemokines and chemokine receptors.

Conclusion: In our study, we identified and established a prognostic signature of immune-related LNPEP in OV, which will be of great value in predicting the prognosis of clinical trials and may become a new therapeutic target for immunological research and potential prognostic biomarker in OV.

背景:卵巢癌(OV)的肿瘤内在异质性和肿瘤微环境(TME)影响免疫治疗的疗效和患者预后。亮氨酸和胱氨酸氨基肽酶(LNPEP)编码锌依赖的氨基肽酶,该酶已被证明参与囊泡介导的转运和I类MHC介导的抗原加工和递呈。然而,LNPEP在OV TME中的作用及其潜在的分子机制尚未确定。因此,我们旨在研究一种可能有助于识别卵巢癌TME异质性的预后生物标志物。方法:利用生物信息学数据库对LNPEP的表达谱和免疫浸润进行研究。对LNPEP的生存数据和相互作用物进行生物信息学分析,以预测LNPEP在OV中的预后价值。Western blot和免疫组化检测LNPEP蛋白水平。结果:基于TCGA数据,我们的数据显示LNPEP mRNA在卵巢癌中的表达明显低于癌旁组织,与蛋白水平相反。重要的是,高LNPEP表达与OV患者的不良预后相关。此外,Cox回归分析显示LNPEP是OV的独立预后因素。GO和KEGG通路分析表明,LNPEP共表达基因主要与多种免疫相关通路相关,包括Th1和Th2细胞分化、Th17细胞分化、免疫调节相互作用等。我们的数据还表明,LNPEP的表达与免疫浸润水平、免疫调节剂、趋化因子和趋化因子受体密切相关。结论:本研究鉴定并建立了OV中免疫相关LNPEP的预后标志,对临床试验的预后预测具有重要价值,可能成为OV免疫学研究的新的治疗靶点和潜在的预后生物标志物。
{"title":"Leucyl and Cystinyl Aminopeptidase as a Prognostic-Related Biomarker in OV Correlating with Immune Infiltrates.","authors":"Qian Ma,&nbsp;Lei Chang,&nbsp;Wenwen Wang,&nbsp;Lingyi Che,&nbsp;Xiaoqin Song,&nbsp;Gailing Li,&nbsp;Ying Zhang,&nbsp;Yibing Chen,&nbsp;Zhuoyu Gu,&nbsp;Xin Ge","doi":"10.2147/PGPM.S400145","DOIUrl":"https://doi.org/10.2147/PGPM.S400145","url":null,"abstract":"<p><strong>Background: </strong>It was indicated that tumor intrinsic heterogeneity and the tumor microenvironment (TME) of ovarian cancer (OV) influence immunotherapy efficacy and patient outcomes. Leucyl and cystinyl aminopeptidase (LNPEP) encodes a zinc-dependent aminopeptidase, which has been proved to participant in the vesicle-mediated transport and class I MHC mediated antigen processing and presentation. However, the function of LNPEP in TME of OV and its potential molecular mechanisms have not been determined. Therefore, we aimed to investigate a prognostic biomarker which may be helpful in identifying TME heterogeneity of ovarian cancer.</p><p><strong>Methods: </strong>In this study, bioinformatics databases were used to explore the expression profile and immune infiltration of LNPEP. Bioinformatics analyses of survival data and interactors of LNPEP were conducted to predict the prognostic value of LNPEP in OV. The protein levels of LNPEP were validated by Western blot and immunohistochemistry.</p><p><strong>Results: </strong>Based on the TCGA data, our data displayed that the mRNA expression of LNPEP was markedly down-regulated in ovarian cancer than that in para-cancer tissues, contrary to the protein level. Importantly, high LNPEP expression was associated with poor prognosis in patients with OV. Furthermore, Cox regression analysis showed that LNPEP was an independent prognostic factor in OV. GO and KEGG pathway analyses indicated the co-expressed genes of LNPEP were mainly related to a variety of immune-related pathways, including Th1 and Th2 cell differentiation, Th17 cell differentiation, and immunoregulatory interaction. Our data also demonstrated that the expression of LNPEP was strongly correlated with immune infiltration levels, immunomodulators, chemokines and chemokine receptors.</p><p><strong>Conclusion: </strong>In our study, we identified and established a prognostic signature of immune-related LNPEP in OV, which will be of great value in predicting the prognosis of clinical trials and may become a new therapeutic target for immunological research and potential prognostic biomarker in OV.</p>","PeriodicalId":56015,"journal":{"name":"Pharmacogenomics & Personalized Medicine","volume":"16 ","pages":"551-568"},"PeriodicalIF":1.9,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/63/99/pgpm-16-551.PMC10244028.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9613502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Exploring the Correlation Between GPR176, a Potential Target Gene of Gastric Cancer, and Immune Cell Infiltration. 胃癌潜在靶基因GPR176与免疫细胞浸润的相关性研究
IF 1.9 4区 医学 Q3 PHARMACOLOGY & PHARMACY Pub Date : 2023-01-01 DOI: 10.2147/PGPM.S411199
Xianhua Gu, Honghong Shen, Zheng Xiang, Xinwei Li, Yue Zhang, Rong Zhang, Fang Su, Zishu Wang

Introduction: GPR176, an orphan G protein-coupled receptor (GPCR), is essential for the progression of gastrointestinal cancers. However, it is still unclear how GPR176 affects tumor immunity and patient prognosis in gastric cancer (GC).

Methods: The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) were searched in this investigation to assess the expression patterns of GPR176 in GC tissues and normal gastric mucosa. The findings were further verified using immunohistochemical tests and quantitative Real-Time Polymerase Chain Reaction (qRT-PCR). The Kaplan-Meier method, univariate logistic regression, and Cox regression were then used to investigate the relationship between GPR176 and clinical traits. Additionally, the potential correlation between GPR176, immune checkpoint genes, and immune cell infiltration levels was investigated.

Results: As per the research findings, GC tissues had higher levels of GPR176 than normal tissues. Additionally, individuals with high expression of GPR176 had a worse 10-year overall survival (OS), in contrast with those having a low expression of GPR176 (p < 0.001). The OS of GC can be predicted using a validated nomogram model. The expression of GPR176 demonstrated a negative correlation with CD8+ T cells. When compared to the low-expression group of GPR176, Tumor Immune Dysfunction and Exclusion (TIDE) analysis demonstrated that the high-expression group had a considerably higher risk of immune evasion. A remarkable difference (variation) was observed in the levels of GPR176 expression across both groups, ie, low and high-risk groups, as determined by the immune phenomenon scores (IPS) immunotherapy assessment.

Conclusion: By examining GPR176 from various biological perspectives, it was determined that GPR176 can act as a predictive biomarker for poor patient prognosis in GC. Additionally, it was observed that GPR176 is capable of suppressing the proliferation of CD8+ T cells and facilitating immune evasion.

GPR176是一种孤儿G蛋白偶联受体(GPCR),对胃肠道癌症的进展至关重要。然而,GPR176在胃癌(GC)中如何影响肿瘤免疫和患者预后尚不清楚。方法:检索肿瘤基因组图谱(Cancer Genome Atlas, TCGA)和基因表达图谱(Gene Expression Omnibus, GEO),分析GPR176在胃癌组织和正常胃黏膜中的表达规律。通过免疫组织化学测试和定量实时聚合酶链反应(qRT-PCR)进一步验证了这一发现。采用Kaplan-Meier法、单因素logistic回归和Cox回归分析GPR176与临床特征的关系。此外,我们还研究了GPR176、免疫检查点基因和免疫细胞浸润水平之间的潜在相关性。结果:GC组织中GPR176水平高于正常组织。此外,与GPR176低表达个体相比,GPR176高表达个体的10年总生存率(OS)更差(p < 0.001)。GC的OS可以用一个经过验证的nomogram模型来预测。GPR176的表达与CD8+ T细胞呈负相关。与GPR176低表达组相比,肿瘤免疫功能障碍和排斥(Tumor Immune Dysfunction and Exclusion, TIDE)分析显示,高表达组免疫逃避的风险明显更高。通过免疫现象评分(IPS)免疫治疗评估,观察到GPR176在两组(即低高危组)中的表达水平有显著差异(变异)。结论:从多个生物学角度对GPR176进行检测,确定GPR176可作为GC患者预后不良的预测生物标志物。此外,观察到GPR176能够抑制CD8+ T细胞的增殖并促进免疫逃避。
{"title":"Exploring the Correlation Between <i>GPR176</i>, a Potential Target Gene of Gastric Cancer, and Immune Cell Infiltration.","authors":"Xianhua Gu,&nbsp;Honghong Shen,&nbsp;Zheng Xiang,&nbsp;Xinwei Li,&nbsp;Yue Zhang,&nbsp;Rong Zhang,&nbsp;Fang Su,&nbsp;Zishu Wang","doi":"10.2147/PGPM.S411199","DOIUrl":"https://doi.org/10.2147/PGPM.S411199","url":null,"abstract":"<p><strong>Introduction: </strong><i>GPR176</i>, an orphan G protein-coupled receptor (GPCR), is essential for the progression of gastrointestinal cancers. However, it is still unclear how <i>GPR176</i> affects tumor immunity and patient prognosis in gastric cancer (GC).</p><p><strong>Methods: </strong>The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) were searched in this investigation to assess the expression patterns of <i>GPR176</i> in GC tissues and normal gastric mucosa. The findings were further verified using immunohistochemical tests and quantitative Real-Time Polymerase Chain Reaction (qRT-PCR). The Kaplan-Meier method, univariate logistic regression, and Cox regression were then used to investigate the relationship between <i>GPR176</i> and clinical traits. Additionally, the potential correlation between <i>GPR176</i>, immune checkpoint genes, and immune cell infiltration levels was investigated.</p><p><strong>Results: </strong>As per the research findings, GC tissues had higher levels of <i>GPR176</i> than normal tissues. Additionally, individuals with high expression of <i>GPR176</i> had a worse 10-year overall survival (OS), in contrast with those having a low expression of <i>GPR176</i> (<i>p</i> < 0.001). The OS of GC can be predicted using a validated nomogram model. The expression of <i>GPR176</i> demonstrated a negative correlation with CD8+ T cells. When compared to the low-expression group of <i>GPR176</i>, Tumor Immune Dysfunction and Exclusion (TIDE) analysis demonstrated that the high-expression group had a considerably higher risk of immune evasion. A remarkable difference (variation) was observed in the levels of <i>GPR176</i> expression across both groups, ie, low and high-risk groups, as determined by the immune phenomenon scores (IPS) immunotherapy assessment.</p><p><strong>Conclusion: </strong>By examining <i>GPR176</i> from various biological perspectives, it was determined that <i>GPR176</i> can act as a predictive biomarker for poor patient prognosis in GC. Additionally, it was observed that <i>GPR176</i> is capable of suppressing the proliferation of CD8+ T cells and facilitating immune evasion.</p>","PeriodicalId":56015,"journal":{"name":"Pharmacogenomics & Personalized Medicine","volume":"16 ","pages":"519-535"},"PeriodicalIF":1.9,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/43/db/pgpm-16-519.PMC10241216.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9645597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Exploration into Plasma Hsa_circ_0052184 as a New Biomarker of Colorectal Cancer Prognosis. 血浆Hsa_circ_0052184作为结直肠癌预后新标志物的探讨
IF 1.9 4区 医学 Q3 PHARMACOLOGY & PHARMACY Pub Date : 2023-01-01 DOI: 10.2147/PGPM.S413451
Enqi Zheng, Deshuang Xiao

Background: Circular RNAs (circRNAs) are strong modulators of tumor pathology. Herein, our goal was to examine the plasma hsa_circ_0052184 content among colorectal cancer (CRC) patients, and assess its association with patient clinicopathological profile and diagnostic values.

Methods: Overall, we collected 228 presurgical CRC and 146 normal plasma samples from The First People's Hospital of Wenling. Circulating hsa_circ_0052184 levels were assessed via qRT-PCR, and the diagnostic prediction was conducted with the receiver operating characteristic (ROC) curve.

Results: Relative to healthy controls, CRC patients exhibited markedly enhanced circulating hsa_circ_0052184 levels, which were closely correlated with advanced stage of disease and worse outcome. Based on our uni- (UA) and multivariate assessments (MA), elevated hsa_circ_0052184 levels were a stand-alone predictor of poor prognosis. The ROC curve depicted an area under the curve (AUC) for CRC diagnosis to be 0.9072.

Conclusion: Circulating hsa_circ_0052184 is a potential bioindicator of CRC outcome.

背景:环状rna (circRNAs)是肿瘤病理的强调节剂。本研究目的是检测结直肠癌(CRC)患者血浆hsa_circ_0052184的含量,并评估其与患者临床病理特征和诊断价值的关系。方法:收集温岭市第一人民医院结直肠癌术前228例,正常血浆146例。采用qRT-PCR检测循环hsa_circ_0052184水平,采用受试者工作特征(ROC)曲线进行诊断预测。结果:与健康对照相比,结直肠癌患者外周血hsa_circ_0052184水平明显升高,与病情进展及预后差密切相关。基于我们的单(UA)和多变量评估(MA), hsa_circ_0052184水平升高是预后不良的独立预测因子。ROC曲线显示CRC诊断的曲线下面积(AUC)为0.9072。结论:循环hsa_circ_0052184是CRC预后的潜在生物指标。
{"title":"Exploration into Plasma Hsa_circ_0052184 as a New Biomarker of Colorectal Cancer Prognosis.","authors":"Enqi Zheng,&nbsp;Deshuang Xiao","doi":"10.2147/PGPM.S413451","DOIUrl":"https://doi.org/10.2147/PGPM.S413451","url":null,"abstract":"<p><strong>Background: </strong>Circular RNAs (circRNAs) are strong modulators of tumor pathology. Herein, our goal was to examine the plasma hsa_circ_0052184 content among colorectal cancer (CRC) patients, and assess its association with patient clinicopathological profile and diagnostic values.</p><p><strong>Methods: </strong>Overall, we collected 228 presurgical CRC and 146 normal plasma samples from The First People's Hospital of Wenling. Circulating hsa_circ_0052184 levels were assessed via qRT-PCR, and the diagnostic prediction was conducted with the receiver operating characteristic (ROC) curve.</p><p><strong>Results: </strong>Relative to healthy controls, CRC patients exhibited markedly enhanced circulating hsa_circ_0052184 levels, which were closely correlated with advanced stage of disease and worse outcome. Based on our uni- (UA) and multivariate assessments (MA), elevated hsa_circ_0052184 levels were a stand-alone predictor of poor prognosis. The ROC curve depicted an area under the curve (AUC) for CRC diagnosis to be 0.9072.</p><p><strong>Conclusion: </strong>Circulating hsa_circ_0052184 is a potential bioindicator of CRC outcome.</p>","PeriodicalId":56015,"journal":{"name":"Pharmacogenomics & Personalized Medicine","volume":"16 ","pages":"589-597"},"PeriodicalIF":1.9,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/74/77/pgpm-16-589.PMC10275319.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9654390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Pyroptosis and Inflammasome-Related Genes-NLRP3, NLRC4 and NLRP7 Polymorphisms Were Associated with Risk of Lung Cancer. 焦亡和炎性小体相关基因nlrp3、NLRC4和NLRP7多态性与肺癌风险相关
IF 1.9 4区 医学 Q3 PHARMACOLOGY & PHARMACY Pub Date : 2023-01-01 DOI: 10.2147/PGPM.S424326
Xin Jing, Yuhui Yun, Xiang Ji, Ende Yang, Pei Li

Background: Cancer development and tumor immune microenvironment remodeling are closely linked to pyroptosis and inflammasome activation. However, little information is available in single nucleotide polymorphisms (SNPs) in pyroptosis and inflammasome-related genes in patients with lung cancer. This study aims to evaluate the associations between pyroptosis-related gene (NLRP3, NLRC4, and NLRP7) polymorphisms and the risk of lung cancer.

Methods: The MassARRAY platform was used to genotype six SNPs of the NLRP3, NLRC4, and NLRP7 genes in 660 lung cancer cases and 660 controls.

Results: Individuals with rs35829419-A, rs385076-C, and rs775882-T alleles exhibited a higher risk of lung cancer (p < 0.01), while rs212704-T appears protective (p = 0.006). The rs35829419-AA, rs385076-TC/CC, and rs775882-CT/TT genotypes were associated with various degrees of elevated risk of lung cancer (p<0.02), whereas rs212704-TT was associated with a reduced risk of the disease (p=0.014). Genetic models analysis showed that rs35829419, rs385076, and rs775882 was associated with an increased risk of lung cancer, while rs212704 was related to a reduced risk in all three models (p < 0.05). The four SNPs remained significant in smoker and nonsmoker subgroups (p < 0.05). However, rs35829419 was correlated with risk of adenocarcinoma and small cell lung cancer, and rs212704 was only protective for squamous cell carcinoma. The rs385076 and rs775882 were associated with all three pathological types (p < 0.01).

Conclusion: Besides providing candidate markers for identification of high-risk populations and early prevention of the disease, our research also provided new insight into anti-tumor strategies targeting inflammasomes and pyroptosis.

背景:肿瘤的发展和肿瘤免疫微环境重塑与焦亡和炎性体活化密切相关。然而,关于肺癌患者焦亡和炎性小体相关基因的单核苷酸多态性(snp)的信息很少。本研究的目的是评估热作用相关基因(NLRP3、NLRC4和NLRP7)多态性与肺癌风险的关系。方法:利用MassARRAY平台对660例肺癌患者和660例对照者的NLRP3、NLRC4和NLRP7基因的6个snp进行基因分型。结果:携带rs35829419-A、rs385076-C和rs775882-T等位基因的个体患肺癌的风险较高(p < 0.01),而携带rs212704-T等位基因的个体具有保护作用(p = 0.006)。rs35829419-AA、rs385076-TC/CC和rs775882-CT/TT基因型与不同程度的肺癌风险升高相关(pp=0.014)。遗传模型分析显示,rs35829419、rs385076和rs775882与肺癌风险增加相关,而rs212704与肺癌风险降低相关(p < 0.05)。这4个snp在吸烟者和非吸烟者亚组中仍然显著(p < 0.05)。然而,rs35829419与腺癌和小细胞肺癌的风险相关,rs212704仅对鳞状细胞癌有保护作用。rs385076和rs775882与3种病理类型均相关(p < 0.01)。结论:我们的研究除了为高危人群的识别和疾病的早期预防提供候选标志物外,还为针对炎症小体和焦亡的抗肿瘤策略提供了新的见解。
{"title":"Pyroptosis and Inflammasome-Related Genes-<i>NLRP3, NLRC4</i> and <i>NLRP7</i> Polymorphisms Were Associated with Risk of Lung Cancer.","authors":"Xin Jing,&nbsp;Yuhui Yun,&nbsp;Xiang Ji,&nbsp;Ende Yang,&nbsp;Pei Li","doi":"10.2147/PGPM.S424326","DOIUrl":"https://doi.org/10.2147/PGPM.S424326","url":null,"abstract":"<p><strong>Background: </strong>Cancer development and tumor immune microenvironment remodeling are closely linked to pyroptosis and inflammasome activation. However, little information is available in single nucleotide polymorphisms (SNPs) in pyroptosis and inflammasome-related genes in patients with lung cancer. This study aims to evaluate the associations between pyroptosis-related gene (<i>NLRP3, NLRC4,</i> and <i>NLRP7</i>) polymorphisms and the risk of lung cancer.</p><p><strong>Methods: </strong>The MassARRAY platform was used to genotype six SNPs of the <i>NLRP3, NLRC4,</i> and <i>NLRP7</i> genes in 660 lung cancer cases and 660 controls.</p><p><strong>Results: </strong>Individuals with rs35829419-A, rs385076-C, and rs775882-T alleles exhibited a higher risk of lung cancer (<i>p</i> < 0.01), while rs212704-T appears protective (<i>p</i> = 0.006). The rs35829419-AA, rs385076-TC/CC, and rs775882-CT/TT genotypes were associated with various degrees of elevated risk of lung cancer (<i>p</i><0.02), whereas rs212704-TT was associated with a reduced risk of the disease (<i>p</i>=0.014). Genetic models analysis showed that rs35829419, rs385076, and rs775882 was associated with an increased risk of lung cancer, while rs212704 was related to a reduced risk in all three models (<i>p</i> < 0.05). The four SNPs remained significant in smoker and nonsmoker subgroups (<i>p</i> < 0.05). However, rs35829419 was correlated with risk of adenocarcinoma and small cell lung cancer, and rs212704 was only protective for squamous cell carcinoma. The rs385076 and rs775882 were associated with all three pathological types (<i>p</i> < 0.01).</p><p><strong>Conclusion: </strong>Besides providing candidate markers for identification of high-risk populations and early prevention of the disease, our research also provided new insight into anti-tumor strategies targeting inflammasomes and pyroptosis.</p>","PeriodicalId":56015,"journal":{"name":"Pharmacogenomics & Personalized Medicine","volume":"16 ","pages":"795-804"},"PeriodicalIF":1.9,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/25/87/pgpm-16-795.PMC10464886.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10482816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Clinical Knowledge, Attitude, and Perceptions of Community Pharmacists Towards Pharmacogenomics - A Cross-Sectional Study from Saudi Arabia. 社区药剂师对药物基因组学的临床知识、态度和看法——来自沙特阿拉伯的横断面研究。
IF 1.9 4区 医学 Q3 PHARMACOLOGY & PHARMACY Pub Date : 2023-01-01 DOI: 10.2147/PGPM.S403655
Ziyad Alrabiah, Wajid Syed, Salmeen D Babelghaith, Mohamed N Al Arifi

Background and aims: It is crucial to provide healthcare personnel with the necessary knowledge and understanding of genetic testing and pharmacogenomics. The purpose of this study is to assess the knowledge, attitudes, views, and considerations of Community pharmacists (CPs) about pharmacogenomics and genetics.

Methods and materials: A cross-sectional web-based study was conducted among practicing pharmacists Between January and February of 2022. Participants were recruited through a convenient sampling technique. A total of 23 item questionnaires were used to assess the Knowledge Attitudes, Views, and Considerations toward Pharmacogenomics among pharmacists.

Results: The mean age of the CPs were 28.45±7.29(Std). Among the CPs, 38.4% (98 of 255) of them were correctly identified human chromosomes, and the majority of them 73.3% knew that adverse reactions can be caused by genetic changes in the human body. A total of 194 CPs agreed that certain drugs can be affected by genetic changes in the patient. In this study, one-third (33%) of the CPs were found to have good knowledge, while most (66.3%) of the CPs were found poor knowledge of pharmacogenomics and genetics. Furthermore, the knowledge score is significantly different concerning the qualification of the CPs (p=0.0001).

Conclusion: The current findings, demonstrated a majority of the CPs found a lack of knowledge and understanding regarding pharmacogenomics and its perspectives, there is a need to increase awareness among CPs to reduce the knowledge gap of pharmacogenomics and genetics.

背景和目的:向卫生保健人员提供基因检测和药物基因组学的必要知识和理解是至关重要的。本研究的目的是评估社区药剂师(CPs)对药物基因组学和遗传学的知识、态度、观点和考虑。方法与材料:对2022年1 - 2月执业药师进行基于网络的横断面研究。参与者是通过方便的抽样技术招募的。采用23份问卷调查药师对药物基因组学的知识、态度、观点和考虑。结果:cp的平均年龄为28.45±7.29(Std)。在这些CPs中,有38.4%的人(255人中有98人)正确识别了人类染色体,其中大多数人(73.3%)知道人体基因变化会引起不良反应。共有194名CPs同意某些药物会受到患者基因变化的影响。在本研究中,三分之一(33%)的CPs对药物基因组学和遗传学知识了解较好,而大多数(66.3%)的CPs对药物基因组学和遗传学知识了解较差。此外,知识得分在CPs的资格方面存在显著差异(p=0.0001)。结论:目前的研究结果表明,大多数CPs对药物基因组学及其观点的认识和理解不足,需要提高CPs的认识,以缩小药物基因组学和遗传学的知识差距。
{"title":"Clinical Knowledge, Attitude, and Perceptions of Community Pharmacists Towards Pharmacogenomics - A Cross-Sectional Study from Saudi Arabia.","authors":"Ziyad Alrabiah,&nbsp;Wajid Syed,&nbsp;Salmeen D Babelghaith,&nbsp;Mohamed N Al Arifi","doi":"10.2147/PGPM.S403655","DOIUrl":"https://doi.org/10.2147/PGPM.S403655","url":null,"abstract":"<p><strong>Background and aims: </strong>It is crucial to provide healthcare personnel with the necessary knowledge and understanding of genetic testing and pharmacogenomics. The purpose of this study is to assess the knowledge, attitudes, views, and considerations of Community pharmacists (CPs) about pharmacogenomics and genetics.</p><p><strong>Methods and materials: </strong>A cross-sectional web-based study was conducted among practicing pharmacists Between January and February of 2022. Participants were recruited through a convenient sampling technique. A total of 23 item questionnaires were used to assess the Knowledge Attitudes, Views, and Considerations toward Pharmacogenomics among pharmacists.</p><p><strong>Results: </strong>The mean age of the CPs were 28.45±7.29(Std). Among the CPs, 38.4% (98 of 255) of them were correctly identified human chromosomes, and the majority of them 73.3% knew that adverse reactions can be caused by genetic changes in the human body. A total of 194 CPs agreed that certain drugs can be affected by genetic changes in the patient. In this study, one-third (33%) of the CPs were found to have good knowledge, while most (66.3%) of the CPs were found poor knowledge of pharmacogenomics and genetics. Furthermore, the knowledge score is significantly different concerning the qualification of the CPs (<i>p</i>=0.0001).</p><p><strong>Conclusion: </strong>The current findings, demonstrated a majority of the CPs found a lack of knowledge and understanding regarding pharmacogenomics and its perspectives, there is a need to increase awareness among CPs to reduce the knowledge gap of pharmacogenomics and genetics.</p>","PeriodicalId":56015,"journal":{"name":"Pharmacogenomics & Personalized Medicine","volume":"16 ","pages":"433-441"},"PeriodicalIF":1.9,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/53/b9/pgpm-16-433.PMC10179052.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9829685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Are We Ready for Whole Population Genomic Sequencing of Asymptomatic Newborns? 我们准备好对无症状新生儿进行全群体基因组测序了吗?
IF 1.9 4区 医学 Q3 PHARMACOLOGY & PHARMACY Pub Date : 2023-01-01 DOI: 10.2147/PGPM.S376083
Danya F Vears, Julian Savulescu, John Christodoulou, Meaghan Wall, Ainsley J Newson

The introduction of genomic sequencing technologies into routine newborn screening programs in some form is not only inevitable but also already occurring in some settings. The question is therefore not "if" but "when and how" genomic newborn screening (GNBS) should be implemented. In April 2022, the Centre for Ethics of Paediatric Genomics held a one-day symposium exploring ethical issues relating to the use of genomic sequencing in a range of clinical settings. This review article synthesises the panel discussion and presents both the potential benefits of wide-scale implementation of genomic newborn screening, as well as its practical and ethical issues, including obtaining appropriate consent, and health system implications. A more in-depth understanding of the barriers associated with implementing genomic newborn screening is critical to the success of GNBS programs, both from a practical perspective and also in order to maintain public trust in an important public health initiative.

以某种形式将基因组测序技术引入常规新生儿筛查计划不仅是不可避免的,而且已经在某些情况下发生了。因此,问题不是“是否”,而是“何时以及如何”实施新生儿基因组筛查。2022年4月,儿科基因组学伦理中心举行了为期一天的研讨会,探讨与在一系列临床环境中使用基因组测序有关的伦理问题。这篇综述文章综合了小组讨论,并提出了大规模实施新生儿基因组筛查的潜在好处,以及它的实际和伦理问题,包括获得适当的同意,以及卫生系统的影响。更深入地了解与实施新生儿基因组筛查相关的障碍对于GNBS计划的成功至关重要,无论是从实际的角度来看,还是为了保持公众对一项重要公共卫生倡议的信任。
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引用次数: 3
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Pharmacogenomics & Personalized Medicine
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