Pub Date : 2022-09-01DOI: 10.4067/s0719-81322022000300145
Katherine García-Livia, Maria Valladares Salmerón, S. Pacheco, B. Valladares, P. Foronda
. Metastrongylid species infecting wild and domestic cats worldwide are increasingly being reported. Between 2017 and 2019, a total of 202 faecal samples of domestic cats from the island of Tenerife (Canary Islands, Spain) were analysed by microscopy and molecular techniques. Morphological analyses showed that 8.91% (18/202) of the faecal samples presented first stage larvae (L1) of metastrongylid species. Total DNA was isolated and tested by PCR targeting a 508 bp fragment of the ITS-2 gene. The nucleotide sequences obtained showed high homology (100%) with the species Oslerus rostratus . This work contributes to the knowledge of the wide distribution of O. rostratus worldwide , being Tenerife (Canary Islands, Spain), close to the African continent, the new geographic location for this metastrongylid species. Further molecular studies involving new geographic areas from the island of Tenerife, as well as neighbouring islands, are needed to provide relevant insight and better understand epidemiology of O. rostratus metastrongylid species wild and domestic cats from the Canary Islands.
{"title":"Report of Oslerus rostratus (Strongylida: Filaroididae) in cats from the Canary Islands, Spain","authors":"Katherine García-Livia, Maria Valladares Salmerón, S. Pacheco, B. Valladares, P. Foronda","doi":"10.4067/s0719-81322022000300145","DOIUrl":"https://doi.org/10.4067/s0719-81322022000300145","url":null,"abstract":". Metastrongylid species infecting wild and domestic cats worldwide are increasingly being reported. Between 2017 and 2019, a total of 202 faecal samples of domestic cats from the island of Tenerife (Canary Islands, Spain) were analysed by microscopy and molecular techniques. Morphological analyses showed that 8.91% (18/202) of the faecal samples presented first stage larvae (L1) of metastrongylid species. Total DNA was isolated and tested by PCR targeting a 508 bp fragment of the ITS-2 gene. The nucleotide sequences obtained showed high homology (100%) with the species Oslerus rostratus . This work contributes to the knowledge of the wide distribution of O. rostratus worldwide , being Tenerife (Canary Islands, Spain), close to the African continent, the new geographic location for this metastrongylid species. Further molecular studies involving new geographic areas from the island of Tenerife, as well as neighbouring islands, are needed to provide relevant insight and better understand epidemiology of O. rostratus metastrongylid species wild and domestic cats from the Canary Islands.","PeriodicalId":56042,"journal":{"name":"Austral Journal of Veterinary Sciences","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48198750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-01DOI: 10.4067/s0719-81322022000300093
Eduardo Hernández‐Cuellar, A. Guerrero-Barrera, F. J. Avelar-gonzález, J. M. Díaz, Jesús Chávez‐Reyes, Alfredo Salazar de Santiago
{"title":"Adhesion mechanisms of Actinobacillus pleuropneumoniae to the porcine respiratory system and biofilm formation","authors":"Eduardo Hernández‐Cuellar, A. Guerrero-Barrera, F. J. Avelar-gonzález, J. M. Díaz, Jesús Chávez‐Reyes, Alfredo Salazar de Santiago","doi":"10.4067/s0719-81322022000300093","DOIUrl":"https://doi.org/10.4067/s0719-81322022000300093","url":null,"abstract":"","PeriodicalId":56042,"journal":{"name":"Austral Journal of Veterinary Sciences","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43409844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.4067/s0719-81322022000100009
S. M. Aboelhadid, Shymaa A Hashem, El-Sayed M Abdel-Kafy, A. Abdel-Baki, S. Al-Quraishy, Ahmed Hussien Abed, A. Kamel
. Coccidian infection may promote the proliferation of gut bacteria of the family Enterobacteriaceae. Bacterial infections by members of this family in rabbits can induce a negative impact on their health and lead to high mortality, especially in young individuals. This study aimed to investigate the effect of prebiotic treatment on Escherichia coli and Salmonella species during natural intestinal coccidiosis in rabbits. Forty-five rabbits were selected from a rabbit farm in Beni-Suef, Egypt. Out of the 45 rabbits, 15 were coccidia-free and used as the negative control group (NC group) and 30 were naturally infected with coccidia. The infected rabbits were randomly divided into two equal groups, the positive control group (PC) and the prebiotic treated group (PT) which was orally treated with prebiotic for 8 successive days. Faecal oocyst count was assessed daily during the course of treatment. At 8 days post-treatment, 5 rabbits from each group were euthanised for the microbiological examination of the intestinal tract. On day 8 post-treatment, the PT group showed a significant ( P ≤0.05) reduction in the oocyst count (5.33 ×10 3 ± 0.89) with a significant ( P ≤0.05) decline in the prevalence of E. coli and Salmonella (66.7 % and 26.4%, respectively). The PC group showed highly a significant oocyst count (21.67×10 3 ± 0.82 OPG), with a significant increase in the prevalence of E. coli and Salmonella (86.7 % and 46.7 %, respectively). The NC group remained coccidian free and exhibited only E. coli with no Salmonella infection. The in vitro susceptibility test showed that E. coli isolates were highly resistant to most of the tested antimicrobials while Salmonella isolates showed variable resistance profiles. In conclusion, the prebiotic treatment significantly reduced the prevalence of E. coli and Salmonella infections coexisted with intestinal coccidiosis naturally infected rabbits.
{"title":"Prevalence and antimicrobial sensitivity of Escherichia coli and Salmonella species in field cases of rabbit intestinal coccidiosis treated with prebiotic","authors":"S. M. Aboelhadid, Shymaa A Hashem, El-Sayed M Abdel-Kafy, A. Abdel-Baki, S. Al-Quraishy, Ahmed Hussien Abed, A. Kamel","doi":"10.4067/s0719-81322022000100009","DOIUrl":"https://doi.org/10.4067/s0719-81322022000100009","url":null,"abstract":". Coccidian infection may promote the proliferation of gut bacteria of the family Enterobacteriaceae. Bacterial infections by members of this family in rabbits can induce a negative impact on their health and lead to high mortality, especially in young individuals. This study aimed to investigate the effect of prebiotic treatment on Escherichia coli and Salmonella species during natural intestinal coccidiosis in rabbits. Forty-five rabbits were selected from a rabbit farm in Beni-Suef, Egypt. Out of the 45 rabbits, 15 were coccidia-free and used as the negative control group (NC group) and 30 were naturally infected with coccidia. The infected rabbits were randomly divided into two equal groups, the positive control group (PC) and the prebiotic treated group (PT) which was orally treated with prebiotic for 8 successive days. Faecal oocyst count was assessed daily during the course of treatment. At 8 days post-treatment, 5 rabbits from each group were euthanised for the microbiological examination of the intestinal tract. On day 8 post-treatment, the PT group showed a significant ( P ≤0.05) reduction in the oocyst count (5.33 ×10 3 ± 0.89) with a significant ( P ≤0.05) decline in the prevalence of E. coli and Salmonella (66.7 % and 26.4%, respectively). The PC group showed highly a significant oocyst count (21.67×10 3 ± 0.82 OPG), with a significant increase in the prevalence of E. coli and Salmonella (86.7 % and 46.7 %, respectively). The NC group remained coccidian free and exhibited only E. coli with no Salmonella infection. The in vitro susceptibility test showed that E. coli isolates were highly resistant to most of the tested antimicrobials while Salmonella isolates showed variable resistance profiles. In conclusion, the prebiotic treatment significantly reduced the prevalence of E. coli and Salmonella infections coexisted with intestinal coccidiosis naturally infected rabbits.","PeriodicalId":56042,"journal":{"name":"Austral Journal of Veterinary Sciences","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70331029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.4067/s0719-81322022000100029
Pamela Olivares-Ferretti, J. Orellana-Cáceres, Luis A. Salazar, F. Fonseca-Salamanca
Fascioliasis is a widely distributed parasitic zoonosis caused by the trematode Fasciola hepatica that affects livestock production and generates high economic losses. In Chilean authorised abattoirs, the infected livestock livers are condemned during the veterinary inspection. This study aims to evaluate the prevalence of fascioliasis in Chile from 2014 to 2016 and also monthly from 2002 to 2015 in livestock (cattle, pig, sheep, horse and goat) slaughtered in abattoirs of La Araucanía region, southern Chile. To do this, the available records on abattoirs provided by the sanitary authority were analysed. A descriptive statistics and trend analysis of the data by jointpoint regression was carried out. The Biobío and La Araucanía regions registered the highest levels of parasitosis in the country recording levels of 59.18 and 44.74%, respectively, and presented 50.03% of the liver condemnation rate in cattle. During the study period, a total of 2,239,164 animals were slaughtered and 40.59% infected livers with F. hepatica were condemned in the 9 existing abattoirs of La Araucanía region. The abattoirs located in the cities of Temuco (51.43%) and Angol (65.09%) recorded the highest percentages of fascioliasis. The cattle species recorded the highest number of slaughtered animals and liver condemnation (54.52%). The presence of the parasite was recorded annually and monthly and it was possible to observe a slight increase in fascioliasis over the years. This study provides updated information on the fascioliasis prevalence in the country and the dynamics of condemnation in endemic areas such as La Araucanía region, which could contribute to the control and prevention of this zoonosis.
{"title":"Fascioliasis prevalence in livestock from abattoirs in southern Chile","authors":"Pamela Olivares-Ferretti, J. Orellana-Cáceres, Luis A. Salazar, F. Fonseca-Salamanca","doi":"10.4067/s0719-81322022000100029","DOIUrl":"https://doi.org/10.4067/s0719-81322022000100029","url":null,"abstract":"Fascioliasis is a widely distributed parasitic zoonosis caused by the trematode Fasciola hepatica that affects livestock production and generates high economic losses. In Chilean authorised abattoirs, the infected livestock livers are condemned during the veterinary inspection. This study aims to evaluate the prevalence of fascioliasis in Chile from 2014 to 2016 and also monthly from 2002 to 2015 in livestock (cattle, pig, sheep, horse and goat) slaughtered in abattoirs of La Araucanía region, southern Chile. To do this, the available records on abattoirs provided by the sanitary authority were analysed. A descriptive statistics and trend analysis of the data by jointpoint regression was carried out. The Biobío and La Araucanía regions registered the highest levels of parasitosis in the country recording levels of 59.18 and 44.74%, respectively, and presented 50.03% of the liver condemnation rate in cattle. During the study period, a total of 2,239,164 animals were slaughtered and 40.59% infected livers with F. hepatica were condemned in the 9 existing abattoirs of La Araucanía region. The abattoirs located in the cities of Temuco (51.43%) and Angol (65.09%) recorded the highest percentages of fascioliasis. The cattle species recorded the highest number of slaughtered animals and liver condemnation (54.52%). The presence of the parasite was recorded annually and monthly and it was possible to observe a slight increase in fascioliasis over the years. This study provides updated information on the fascioliasis prevalence in the country and the dynamics of condemnation in endemic areas such as La Araucanía region, which could contribute to the control and prevention of this zoonosis.","PeriodicalId":56042,"journal":{"name":"Austral Journal of Veterinary Sciences","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70331494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.4067/s0719-81322022000100037
Cristóbal Dorner, Javier Encalada, Jorge Maldonado, J. Palmero
. Compressive myelopathy caused by developmental cervical and thoracic malformation was diagnosed in an 11-month- old Chilean Caballo Raza Chilena colt. The patient evidenced an abnormal wide-based stance, neck muscle atrophy, upside-down neck conformation and tetra ataxia. Ataxia was exacerbated when the colt was walked down and uphill, walked with elevated head, backing and turning in tight circles. The patient showed no improvement following medical therapy, therefore, the colt was euthanised due to a poor prognosis after myelogram findings. Cervical spine malalignment (spondylolisthesis) associated with multi-level compression of the spinal cord was suspected based on cervical radiographs and myelogram findings and was confirmed postmortem. Thoracic vertebral malformation retained cartilage matrix spicules and a flare of the cranial vertebral epiphysis of the first thoracic vertebrae (T1) were also diagnosed at necropsy.
{"title":"Multiple cervical spondylolisthesis and thoracic vertebral malformation in an 11 month-old Chilean Caballo Raza Chilena colt","authors":"Cristóbal Dorner, Javier Encalada, Jorge Maldonado, J. Palmero","doi":"10.4067/s0719-81322022000100037","DOIUrl":"https://doi.org/10.4067/s0719-81322022000100037","url":null,"abstract":". Compressive myelopathy caused by developmental cervical and thoracic malformation was diagnosed in an 11-month- old Chilean Caballo Raza Chilena colt. The patient evidenced an abnormal wide-based stance, neck muscle atrophy, upside-down neck conformation and tetra ataxia. Ataxia was exacerbated when the colt was walked down and uphill, walked with elevated head, backing and turning in tight circles. The patient showed no improvement following medical therapy, therefore, the colt was euthanised due to a poor prognosis after myelogram findings. Cervical spine malalignment (spondylolisthesis) associated with multi-level compression of the spinal cord was suspected based on cervical radiographs and myelogram findings and was confirmed postmortem. Thoracic vertebral malformation retained cartilage matrix spicules and a flare of the cranial vertebral epiphysis of the first thoracic vertebrae (T1) were also diagnosed at necropsy.","PeriodicalId":56042,"journal":{"name":"Austral Journal of Veterinary Sciences","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70331635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.4067/s0719-81322022000100023
Mayelin P. Zayas, Yenis del T. Yen, Gladys P. Naranjo, A. Odio, Daniel L. Cala Delgado
. The safety and protective efficacy of the CPL-0015 Cuban isolate of canine parvovirus type 2 (CPV-2) were evaluated for its possible use as a vaccine strain candidate. The study included a total of 23 healthy Beagle dogs of both sexes, aged 84 days and without specific maternal antibodies against canine parvovirus. Safety was analysed by comparing clinicopathological values, food consumption, body weight, rectal temperature and white blood cell counts for 14 consecutive days between control dogs (n=5) and dogs subcutaneously injected (n=10) with 2 mL (equivalent to two doses) of the CPL-0015 strain with an antigenic titer of 10 6.0 infectious dose 50 in cell culture/mL. The protective effectiveness was determined by measuring and comparing anti-CPV-2 IgG levels and clinical signs during 56 experimental days between control dogs (n=2) and dogs inoculated (n=6) with double doses of 1 mL each, separated by a 21-day interval. All animals were challenged orally on day 35 with the virulent strain Cornell-780916 (10 5.0 infective dose 50 in cell culture/mL). The results showed that the CPL-0015 strain did not negatively impact the physiological condition of the exposed animals. The inoculated and challenged animals showed not only significantly increased levels of anti-CPV-2 IgG ( P <0.05) when compared to days 0, 35, and the control group animals but also had 100% survival without clinical signs of the disease, unlike the control group. It is concluded that CPL-0015 is safe and provides effective protection against homologous virulent strains.
{"title":"Assessment of the CPL-0015 isolate as a vaccine strain for the control of canine parvovirus in Cuba","authors":"Mayelin P. Zayas, Yenis del T. Yen, Gladys P. Naranjo, A. Odio, Daniel L. Cala Delgado","doi":"10.4067/s0719-81322022000100023","DOIUrl":"https://doi.org/10.4067/s0719-81322022000100023","url":null,"abstract":". The safety and protective efficacy of the CPL-0015 Cuban isolate of canine parvovirus type 2 (CPV-2) were evaluated for its possible use as a vaccine strain candidate. The study included a total of 23 healthy Beagle dogs of both sexes, aged 84 days and without specific maternal antibodies against canine parvovirus. Safety was analysed by comparing clinicopathological values, food consumption, body weight, rectal temperature and white blood cell counts for 14 consecutive days between control dogs (n=5) and dogs subcutaneously injected (n=10) with 2 mL (equivalent to two doses) of the CPL-0015 strain with an antigenic titer of 10 6.0 infectious dose 50 in cell culture/mL. The protective effectiveness was determined by measuring and comparing anti-CPV-2 IgG levels and clinical signs during 56 experimental days between control dogs (n=2) and dogs inoculated (n=6) with double doses of 1 mL each, separated by a 21-day interval. All animals were challenged orally on day 35 with the virulent strain Cornell-780916 (10 5.0 infective dose 50 in cell culture/mL). The results showed that the CPL-0015 strain did not negatively impact the physiological condition of the exposed animals. The inoculated and challenged animals showed not only significantly increased levels of anti-CPV-2 IgG ( P <0.05) when compared to days 0, 35, and the control group animals but also had 100% survival without clinical signs of the disease, unlike the control group. It is concluded that CPL-0015 is safe and provides effective protection against homologous virulent strains.","PeriodicalId":56042,"journal":{"name":"Austral Journal of Veterinary Sciences","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70331296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.4067/s0719-81322022000100017
H. Uribe, F. Lembeye, H. González
. Somatic cell count (SCC) is an indirect measurement to estimate mammary gland health status. This trait provides information regarding the severity of the mammary tissue inflammation in each quarter. Milk samples coming from the farm milk storage vat containing 100,000 to 200,000 cell/mL are considered suspicious, while SCC over 200,000 cell/mL is an indication of subclinical mastitis. Chilean dairy processors penalise farmers monetarily when their bulk tank samples reach levels of 300,000 cell/ mL SCC. The objective of this study was to quantify the additive genetic component of the liability of cows to reach the 300,000 cell/mL threshold. A data set containing the highest SCC test-day record of 10,528 first lactation cows from 15 commercial dairy farms of Los Ríos Region in southern Chile was analysed. The unknown continuous underlying susceptibility of each cow to reach the 300,000 SCC threshold was modelled as a function of a contemporary group formed by the herd, year, and calving season, the regression coefficient of the unknown underlying susceptibility value of a cow on her daily milk yield (MY) and the additive animal genetic effect. Bayesian inference and Gibbs sampling were used to estimate additive and residual variances. The average daily MY and SCC were 17.84±5.25 kg and 125,327±236,297 cell/mL, respectively. The estimated heritability varied from 0.03 to 0.22 and the average was 0.10±0.03. It is concluded that the genetic variability for the susceptibility to reach the 300,000 SCC threshold could be exploited to improve resistance to subclinical mastitis.
{"title":"Estimation of genetic parameters for subclinical mastitis using a threshold model in first parity dairy cows under pasture-based systems of Los Ríos Region in Chile","authors":"H. Uribe, F. Lembeye, H. González","doi":"10.4067/s0719-81322022000100017","DOIUrl":"https://doi.org/10.4067/s0719-81322022000100017","url":null,"abstract":". Somatic cell count (SCC) is an indirect measurement to estimate mammary gland health status. This trait provides information regarding the severity of the mammary tissue inflammation in each quarter. Milk samples coming from the farm milk storage vat containing 100,000 to 200,000 cell/mL are considered suspicious, while SCC over 200,000 cell/mL is an indication of subclinical mastitis. Chilean dairy processors penalise farmers monetarily when their bulk tank samples reach levels of 300,000 cell/ mL SCC. The objective of this study was to quantify the additive genetic component of the liability of cows to reach the 300,000 cell/mL threshold. A data set containing the highest SCC test-day record of 10,528 first lactation cows from 15 commercial dairy farms of Los Ríos Region in southern Chile was analysed. The unknown continuous underlying susceptibility of each cow to reach the 300,000 SCC threshold was modelled as a function of a contemporary group formed by the herd, year, and calving season, the regression coefficient of the unknown underlying susceptibility value of a cow on her daily milk yield (MY) and the additive animal genetic effect. Bayesian inference and Gibbs sampling were used to estimate additive and residual variances. The average daily MY and SCC were 17.84±5.25 kg and 125,327±236,297 cell/mL, respectively. The estimated heritability varied from 0.03 to 0.22 and the average was 0.10±0.03. It is concluded that the genetic variability for the susceptibility to reach the 300,000 SCC threshold could be exploited to improve resistance to subclinical mastitis.","PeriodicalId":56042,"journal":{"name":"Austral Journal of Veterinary Sciences","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70331279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.4067/s0719-81322022000100001
T. Tadich, Sebastián Escobar-Aguirre
. To date, there has been an increase in genome modification biotechnologies that improve production and food security but the process has not been accompanied by the delivery of information about them intended for citizens. This is essential considering that to achieve better health, food security and sustainability these biotechnologies need to be incorporated into production systems. This study aimed to explore perceptions and attitudes of Chilean citizens towards the use of genome modifications with an emphasis on transgenes and genome editing (CRISPR). An electronic questionnaire was applied, and afterwards the results were analysed through descriptive statistics, GLM, Spearman’s correlation and Wilcoxon Signed Rank test. A total of 702 questionnaires were analysed. High awareness of concepts such as transgenic and cloning was reported with CRISPR being the least known term. Most respondents perceived negative effects on health regarding the consumption of genetically modified products, with women having a significantly more negative attitude. Still, a high willingness to use CRISPR for improving animal and human health was reported. When comparing vegetable and animal products that underwent CRISPR or transgenes, the willingness to consume these products was higher for vegetables. The results show that changes in perception can be achieved after providing the definition of CRISPR and transgenic, therefore, consumer education seems to be essential. Science communication focused on making information about genome modification biotechnologies available to citizens could promote more positive attitudes and perceptions and facilitate their future implementation in the country.
{"title":"Citizens’ attitudes and perceptions towards genetically modified food in Chile: Special emphasis in CRISPR technology","authors":"T. Tadich, Sebastián Escobar-Aguirre","doi":"10.4067/s0719-81322022000100001","DOIUrl":"https://doi.org/10.4067/s0719-81322022000100001","url":null,"abstract":". To date, there has been an increase in genome modification biotechnologies that improve production and food security but the process has not been accompanied by the delivery of information about them intended for citizens. This is essential considering that to achieve better health, food security and sustainability these biotechnologies need to be incorporated into production systems. This study aimed to explore perceptions and attitudes of Chilean citizens towards the use of genome modifications with an emphasis on transgenes and genome editing (CRISPR). An electronic questionnaire was applied, and afterwards the results were analysed through descriptive statistics, GLM, Spearman’s correlation and Wilcoxon Signed Rank test. A total of 702 questionnaires were analysed. High awareness of concepts such as transgenic and cloning was reported with CRISPR being the least known term. Most respondents perceived negative effects on health regarding the consumption of genetically modified products, with women having a significantly more negative attitude. Still, a high willingness to use CRISPR for improving animal and human health was reported. When comparing vegetable and animal products that underwent CRISPR or transgenes, the willingness to consume these products was higher for vegetables. The results show that changes in perception can be achieved after providing the definition of CRISPR and transgenic, therefore, consumer education seems to be essential. Science communication focused on making information about genome modification biotechnologies available to citizens could promote more positive attitudes and perceptions and facilitate their future implementation in the country.","PeriodicalId":56042,"journal":{"name":"Austral Journal of Veterinary Sciences","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70331463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-09-01DOI: 10.4067/s0719-81322021000300161
G. M. Mendoza Martinéz, Miguel A. López, Pedro Abel Hernández García, Josué J. Ríos Hilario, Javier González, Adrian Gloria Trujillo
. This study aimed to evaluate the palatability, nutrient digestibility, metabolisable energy (ME) and faecal characteristics of diets in dogs fed increasing levels of herbal phosphatidylcholine (herbal mix) versus an unsupplemented diet (with only 377 mg choline provided by 1 kg food) or choline chloride 1 (2000 mg choline/kg food) in 40 adult dogs. In experiment 1, a palatability test was conducted to make two pairwise comparisons: 0 versus 200; and 0 versus 400 mg/kg herbal mix. In experiment 2, a digestibility test was performed to evaluate herbal mix at 0, 200, 400 and 800 mg/kg and 2000 mg choline provided by choline chloride. Results from experiment 1 indicated that the dogs preferred diets containing herbal mix to the unsupplemented diet ( P <0.05). In experiment 2, nutrient digestibility and faecal characteristics were not influenced by the treatment ( P <0.059). The inclusion of 400 mg/kg of herbal mix increased the ME (quadratic effect, P <0.01). In conclusion, the results of this study indicate that the inclusion of a herbal mix rich in phosphatidylcholine (1.6%) and other methylated metabolites at 400 mg/kg can fully replace choline chloride in dog diets.
{"title":"Effect of the inclusión of herbal phosphatidylcholine on palatability, digestibility and metabolisable energy of the diet in dogs","authors":"G. M. Mendoza Martinéz, Miguel A. López, Pedro Abel Hernández García, Josué J. Ríos Hilario, Javier González, Adrian Gloria Trujillo","doi":"10.4067/s0719-81322021000300161","DOIUrl":"https://doi.org/10.4067/s0719-81322021000300161","url":null,"abstract":". This study aimed to evaluate the palatability, nutrient digestibility, metabolisable energy (ME) and faecal characteristics of diets in dogs fed increasing levels of herbal phosphatidylcholine (herbal mix) versus an unsupplemented diet (with only 377 mg choline provided by 1 kg food) or choline chloride 1 (2000 mg choline/kg food) in 40 adult dogs. In experiment 1, a palatability test was conducted to make two pairwise comparisons: 0 versus 200; and 0 versus 400 mg/kg herbal mix. In experiment 2, a digestibility test was performed to evaluate herbal mix at 0, 200, 400 and 800 mg/kg and 2000 mg choline provided by choline chloride. Results from experiment 1 indicated that the dogs preferred diets containing herbal mix to the unsupplemented diet ( P <0.05). In experiment 2, nutrient digestibility and faecal characteristics were not influenced by the treatment ( P <0.059). The inclusion of 400 mg/kg of herbal mix increased the ME (quadratic effect, P <0.01). In conclusion, the results of this study indicate that the inclusion of a herbal mix rich in phosphatidylcholine (1.6%) and other methylated metabolites at 400 mg/kg can fully replace choline chloride in dog diets.","PeriodicalId":56042,"journal":{"name":"Austral Journal of Veterinary Sciences","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49118950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-09-01DOI: 10.4067/s0719-81322021000300169
D. Echeverry, S. Llanos-Soto, C. Palma, Linda Castillo, Tomás Casanova, I. Troncoso, Manuel Valdés, F. O. Castro, D. GONZÁLEZ-ACUÑA
Routine blood analysis indicated the presence of Mycoplasma-like bodies in a guigna (Leopardus guigna). Evidence of infection with Candidatus Mycoplasma haemominutum was found in blood samples using PCR and DNA sequencing of the 16S rRNA gene of Mycoplasma. Mycoplasma spp. are documented in cats but their role in the transmission of Mycoplasma to guigna populations requires investigation.
{"title":"Mycoplasmal infection in a guigna (Leopardus guigna) from central Chile","authors":"D. Echeverry, S. Llanos-Soto, C. Palma, Linda Castillo, Tomás Casanova, I. Troncoso, Manuel Valdés, F. O. Castro, D. GONZÁLEZ-ACUÑA","doi":"10.4067/s0719-81322021000300169","DOIUrl":"https://doi.org/10.4067/s0719-81322021000300169","url":null,"abstract":"Routine blood analysis indicated the presence of Mycoplasma-like bodies in a guigna (Leopardus guigna). Evidence of infection with Candidatus Mycoplasma haemominutum was found in blood samples using PCR and DNA sequencing of the 16S rRNA gene of Mycoplasma. Mycoplasma spp. are documented in cats but their role in the transmission of Mycoplasma to guigna populations requires investigation.","PeriodicalId":56042,"journal":{"name":"Austral Journal of Veterinary Sciences","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48209466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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