Water resources sustainability is an increasing concern, requiring accurate estimates of the hydrological processes involved. This study evaluates the impacts of climate change (CC) and land use (LU) in a river basin in the Brazilian semi-arid. Using the Soil & Water Assessment Tool (SWAT), calibration/ validation was carried out based on limited measurements for discharge and using complementary remote sensing information for evapotranspiration (ET), as well as experimental data for soil moisture (SM). The evapotranspiration data used for calibration were obtained from the MOD16A2 product, at sub-basin scale. Principal component analysis was used to evaluate the interaction between variables. To assess the impacts of CC e and LU two scenarios were investigated: S1 – LU map without insertion of Permanent Preservation Areas (PPA), and S2 – with insertion of PPA. The Regional Climate Models (RCM) Eta-MIROC5 and Eta-HADGEM-ES for the Representative Concentration Pathway (RCP) 4.5 and 8.5 scenarios were adopted. The SWAT model adequately represented evapotranspiration, with Nash-Sutcliffe coefficient (NS) of 0.67 for calibration, and 0.74 for validation. For wet periods, the SM simulated values were similar to the experimental measurements with a coefficient of determination (R²) of 0.68. Complementary information for evapotranspiration and soil moisture across sub-basins successfully allowed consistent spatially distributed hydrological variables to be simulated. The model performance for validation using ET data was higher (NS = 0.77) compared to previous streamflow-only calibration strategies for flow (NS = 0.42) and soil moisture (NS = 0.46). Under the CC scenarios, natural vegetation restoration would compensate reductions in water availability.
The main reason for the appearance of pharmaceutical compounds in the aquatic environment is their regular excretion by humans and animals in an unchanged form or slightly metabolized. Pharmaceuticals limit the habitable living environment for aquatic organisms, because they can be toxic not only to bacteria but also to non-target organisms. Plants of the Lemnoideae subfamily and crustaceans Daphniindae family are widely used as bioindicators in freshwater environmental risk assessments. This study aimed to use biotests (Lemna test and Daphtoxkit) to determine the effect of two pharmaceuticals: antibiotic – Doxycycline (DOX) and semi-synthetic chemotherapeutic drug – Norfloxacin (NOR) on plants Lemna minor and crustaceans Daphnia magna. Standard Lemna test was extended to include pharmaceutical effects on plant chlorophyll content (LCC) and fluorescence (Fo, Fm, and Fv/Fm), and the confirmation of drug toxicity was the biotest Daphtoxkit assessing the immobilization (IM) of organisms. Studies have shown that DOX was more toxic than NOR on tested aquatic organisms: plants and crustaceans. The lowest observed effect concentration (EC20) of DOX and NOR reduced by 20 % LCC and Iy of L. minor was 2.14 and 8.11 mg × L − 1, respectively. The LCC was an early and sensitive indicator of the phytotoxic effects of DOX in L. minor before morphological changes were observed. Confirmation of drug toxicity was the Daphtoxkit. The EC20 (180. min) of DOX and NOR, IM of daphnia by 20 % was 117.18 and 215.42 mg × L − 1, respectively. In conclusion, DOX and NOR in aquatic environments may have significant implications for tested organisms and their ecosystems.