Ecohydrology & Hydrobiology最新文献

Process-based understanding of spur and vegetation effects on channel bank hydrodynamics under submerged and emergent sandbar conditions 水下和突发性沙洲条件下冲刺和植被对河道岸水动力影响的基于过程的认识

IF 2.2 4区环境科学与生态学 Q2 ECOLOGY

Ecohydrology & Hydrobiology

Pub Date : 2026-02-04 DOI: 10.1016/j.ecohyd.2026.100732

Om Prakash Maurya, Saikat Das, Manish Singh Rana, Subashisa Dutta

This study quantifies the hydrodynamic response of structural (spurs) and vegetative (rigid submerged vegetation) interventions in a straight laboratory flume with alternating submerged and emergent sandbar configurations. Six experiments (EX1-EX6) were conducted to evaluate the influence of vegetation density and spur placement on streamwise velocity, turbulent kinetic energy (TKE*), Reynolds shear stress (RSS*), and quadrant-based turbulence structures across six cross-sections. Dense vegetation (lateral and longitudinal spacing = 2.5 cm) reduced near-bank streamwise velocity by up to 45%, with the maximum suppression observed at downstream cross-sections. Spur structures produced localized flow resistance and reduced velocity by up to 30%, but their influence diminished downstream of the spur region. TKE* peaked around 4-5 near spur tips, indicating localized turbulence amplification, while dense vegetation reduced TKE* to 1.5-2, aligning with canopy height and demonstrating its role in damping energy transfer. RSS* values reached approximately 6 near spurs, signifying enhanced downward momentum exchange, whereas dense vegetation reduced RSS* to about 2, confirming suppression of vertical turbulence. Quadrant analysis showed that spurs intensified outward (Q1) and sweep (Q4) bursts, whereas dense vegetation enhanced ejection (Q2) events, promoting upward momentum transfer and reducing near-bed shear stress. Overall, dense vegetation proved more effective than structural measures in stabilizing flow and reducing turbulence, offering a sustainable approach for riverbank protection and sediment control in channel systems.

本研究量化了一个垂直实验室水槽中结构（刺）和植物（刚性淹没植被）干预的水动力响应，其中淹没沙洲和涌现沙洲配置交替进行。通过6个实验（EX1-EX6），研究了植被密度和岩屑放置对6个断面上的流向流速、湍流动能（TKE*）、雷诺剪应力（RSS*）和基于象限的湍流结构的影响。茂密的植被（横向和纵向间距= 2.5 cm）使近岸流向的流速降低了45%，在下游断面观察到最大的抑制。马刺结构产生了局部流动阻力，并使速度降低了30%，但其影响在马刺区域下游减弱。TKE*在4-5附近达到峰值，表明局部湍流放大，而茂密的植被将TKE*降低到1.5-2，与冠层高度一致，表明其在阻尼能量传递中的作用。在马刺附近的RSS*值约为6，表明向下的动量交换增强，而茂密的植被将RSS*降低到2左右，证实了垂直湍流的抑制。象限分析显示，马刺加剧了向外（Q1）和横扫（Q4）爆发，而茂密的植被增强了抛射（Q2）事件，促进了向上的动量传递，降低了近层剪应力。总体而言，茂密植被在稳定水流和减少湍流方面比结构性措施更有效，为河道系统的护岸和控沙提供了可持续的途径。

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引用次数: 0

Double drought phases characterize the high-elevation temporary pond hydroperiod 双干旱期是高海拔临时塘水期的特征

IF 2.2 4区环境科学与生态学 Q2 ECOLOGY

Ecohydrology & Hydrobiology

Pub Date : 2026-01-01 DOI: 10.1016/j.ecohyd.2026.100736

Davide Taurozzi , Massimiliano Scalici

Temporary ponds are among the most widespread lentic ecosystems on Earth, capable of sustaining remarkably high levels of biodiversity. Their defining feature is the hydroperiod, the time span during which water remains in a liquid state. Owing to this ephemeral nature, temporary ponds host rare and specialized communities adapted to the alternation between wet and dry phases. However, high-mountain temporary ponds exhibit a distinctive hydrological cycle. For the first time, we define this pattern as a “double dry phase”, consisting of two distinct dry periods within the same annual cycle. The first is a “classic” summer dry phase, when rising temperatures and scarce precipitation cause complete desiccation. The second is a “frozen” winter dry phase, when ponds, unlike most lakes, freeze entirely due to their shallow depth (typically <8 m). Complete freezing makes these systems biologically inactive, creating a functional drought despite the physical presence of ice. This study provides the first empirical evidence of such a dual-phase hydrological cycle in high-elevation ponds of Central Italy, highlighting their extreme environmental intermittency. These unique ecosystems remain biologically active only for a few months each year, during which liquid water is available. In the context of ongoing climate change, with expected increases in both summer temperatures that could further shorten the liquid-water period, our findings offer a crucial baseline for the recognition and conservation of alpine temporary ponds as singular and vulnerable habitats.

临时池塘是地球上分布最广的生态系统之一，能够维持相当高的生物多样性。它们的定义特征是水周期，即水保持液态的时间跨度。由于这种短暂的性质，临时池塘容纳了适应干湿交替的稀有和专门的群落。然而，高山临时池塘表现出独特的水文循环。我们首次将这种模式定义为“双干期”，即在同一年周期内由两个不同的干期组成。第一个阶段是“典型的”夏季干燥阶段，气温上升和降水稀少导致完全干燥。第二阶段是“冻结”的冬季干期，与大多数湖泊不同，池塘由于深度浅（通常为8米）而完全冻结。完全冻结会使这些系统失去生物活性，造成功能性干旱，尽管冰的物理存在。这项研究提供了意大利中部高海拔池塘中这种双阶段水文循环的第一个经验证据，突出了它们极端的环境间歇性。这些独特的生态系统每年只有几个月保持生物活性，在此期间有液态水可用。在气候持续变化的背景下，预计夏季气温的升高会进一步缩短液态水周期，我们的研究结果为识别和保护高山临时池塘作为单一和脆弱的栖息地提供了重要的基线。

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引用次数: 0

Hydrodynamic modeling as a tool for analyzing the hydrological effects of river maintenance activities 水动力模拟作为分析河流养护活动水文效应的工具

IF 2.2 4区环境科学与生态学 Q2 ECOLOGY

Ecohydrology & Hydrobiology

Pub Date : 2025-12-01 DOI: 10.1016/j.ecohyd.2025.100677

Maria Grodzka-Łukaszewska , Dorota Mirosław-Świątek , Mateusz Grygoruk , Tomasz Krepski , Sylwia Szporak-Wasilewska , Grzegorz Sinicyn , Tomasz Okruszko

Climate change exacerbates water scarcity globally, impacting vital wetland ecosystems that provide crucial ecological services. As a result, peatland areas experience a gradual decrease in water table levels, leading to their degradation and emphasizing the need for effective water level management strategies. This study examines the hydrological and ecological consequences of riverbed maintenance—specifically vegetation mowing—on the Upper Biebrza Valley. Using a coupled hydrodynamic-groundwater flow model supported by an Artificial Neural Network (ANN), we simulated the impacts of different mowing scenarios on groundwater levels, water balance, and vegetation response.

The results show that mowing vegetation from the riverbed temporarily lowers river water levels, which increases the hydraulic gradient and accelerates water outflow from adjacent peatlands. In the most intensive scenario, riverbed mowing led to a short-term increase of up to 24 % in groundwater discharge to the river compared to the reference (non-mowed) condition. However, this effect is temporally limited and site-specific. The impact on groundwater levels and wetland habitats varied significantly between plant communities, with species located closest to the river showing the highest sensitivity to water table decline.

These findings demonstrate that routine riverbed maintenance can negatively affect peatland hydrology and ecosystem resilience, even when applied partially. The study emphasizes the need to reassess standard maintenance practices, particularly in protected and climate-sensitive landscapes.

气候变化加剧了全球水资源短缺，影响了提供重要生态服务的重要湿地生态系统。因此，泥炭地地区的地下水位逐渐下降，导致其退化，并强调需要有效的水位管理战略。本研究考察了比布尔扎上游河谷河床维护（特别是植被修剪）的水文和生态后果。利用人工神经网络（ANN）支持的水动力-地下水耦合模型，模拟了不同刈割情景对地下水位、水分平衡和植被响应的影响。结果表明：刈割植被使河床暂时降低了水位，增加了水力梯度，加速了邻近泥炭地的水流；在最密集的情况下，与参考（未刈割）条件相比，河床刈割导致短期内向河流排放的地下水增加了24%。然而，这种影响是暂时有限的，而且是局部的。不同植物群落对地下水位和湿地生境的影响差异显著，靠近河流的物种对地下水位下降的敏感性最高。这些发现表明，常规的河床维护会对泥炭地水文和生态系统恢复力产生负面影响，即使是部分实施。该研究强调有必要重新评估标准的维护做法，特别是在受保护和气候敏感的景观中。

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引用次数: 0

Polystyrene microbeads in freshwater ecosystems - Ecotoxicological effects on Daphnia magna 淡水生态系统中的聚苯乙烯微珠。对大水蚤的生态毒理学影响

IF 2.2 4区环境科学与生态学 Q2 ECOLOGY

Ecohydrology & Hydrobiology

Pub Date : 2025-12-01 DOI: 10.1016/j.ecohyd.2025.100647

Nina Doskocz , Dominika Skwarska , Katarzyna Affek , Marta Kucharska , Jessica Hua , Monika Załęska-Radziwiłł

The global surge in plastic production has led to widespread contamination of aquatic ecosystems with microplastics. Despite increasing concerns, data on their impact on freshwater species are limited, which hampers comprehensive ecological risk assessments.

This study investigates the effects of polystyrene microparticles on the survival and physiological processes of the freshwater crustacean Daphnia magna. The research evaluates the direct effects of microplastics in acute and chronic exposures, correlating impacts with particle size and concentration, and identifying the locations of microplastic accumulation in the organisms.

Results show that polystyrene microplastics have size- and concentration-dependent negative effects on D. magna, causing particle accumulation in the digestive system, cellular damage, reproductive inhibition, and immobilization. The most toxic particles were 1 μm in size, while 0.5 μm and 3 μm particles exhibited lower acute toxicity. Chronic exposure led to reduction in offspring body length across most microbead sizes. The most pronounced effects were observed for 3 µm particles in terms of the number of neonates, and for 1 µm particles in terms of body length. Transmission electron microscopy revealed that the smallest particles (0.5 μm and 0.75 μm) most effectively penetrated intestinal cells, causing cytoplasmic accumulation, mitochondrial damage, and cell disruption. These findings contribute to improving environmental risk assessments for microplastics in freshwater ecosystems.

全球塑料生产的激增导致水生生态系统受到微塑料的广泛污染。尽管人们越来越关注，但关于它们对淡水物种影响的数据有限，这妨碍了全面的生态风险评估。本研究探讨了聚苯乙烯微粒对淡水甲壳类大水蚤（Daphnia magna）生存和生理过程的影响。该研究评估了微塑料在急性和慢性暴露中的直接影响，将影响与颗粒大小和浓度联系起来，并确定了微塑料在生物体中积聚的位置。结果表明，聚苯乙烯微塑料对D. magna具有粒径和浓度依赖的负面影响，可引起消化系统的颗粒积聚、细胞损伤、生殖抑制和固定化。粒径为1 μm的颗粒毒性最大，0.5 μm和3 μm的颗粒毒性较小。长期暴露导致大多数微珠大小的后代体长减少。3µm颗粒对新生儿数量的影响最为显著，1µm颗粒对体长的影响最为显著。透射电镜显示，最小的颗粒（0.5 μm和0.75 μm）最有效地穿透肠细胞，引起细胞质积聚，线粒体损伤和细胞破坏。这些发现有助于改进淡水生态系统中微塑料的环境风险评估。

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引用次数: 0

Seasonal shifts and habitat complexity: unveiling plankton-meiofauna communities dynamics in artificial reef ecosystems of Haizhou Bay, China 季节变化与生境复杂性：揭示海州湾人工鱼礁生态系统浮游生物群落动态

IF 2.2 4区环境科学与生态学 Q2 ECOLOGY

Ecohydrology & Hydrobiology

Pub Date : 2025-12-01 DOI: 10.1016/j.ecohyd.2025.100668

Ze Zhao , Jikun Lu , Tianhang Jia , Guanghui Fu , Miaomiao Sun , Shuo Zhang , Shike Gao

Artificial reefs (ARs) play a vital role in marine habitat restoration; however, their influence on microbial communities remains poorly understood. In the present study, we investigated seasonal- and habitat-driven variations in plankton and meiofauna communities (herein referred to as microbial communities) in Haizhou Bay, China, by performing 18S rDNA sequencing and co-occurrence network analysis. Samples were collected from AR surfaces and adjacent seawater in spring and autumn, and the environmental parameters, such as temperature, salinity, and depth, were concurrently detected. The results revealed significant α-diversity differences. Autumn bottom waters exhibited the highest richness, whereas AR surfaces exhibited decreased Shannon indices (P < 0.001). β-Diversity analysis revealed distinct community structures between ARs and water columns (P < 0.001), which were largely driven by seasonal shifts in temperature and salinity. Reef surfaces supported complex networks with >80 % positive correlations, suggesting mutualistic interactions. Vertical stratification on ARs led to depth-dependent specialization, with increasing Annelida abundance at deeper layers in spring (P = 0.012). Linear discriminant analysis effect size identified habitat-specific biomarkers, such as Cnidaria in autumn ARs and Ciliophora in spring water, suggesting niche partitioning. Redundancy analysis revealed salinity and temperature as major environmental drivers. Altogether, our findings suggest that ARs enhance biodiversity and ecosystem stability through habitat heterogeneity and modular networks, buffering environmental changes. These findings offer practical insights for optimizing AR designs and formulating strategies to support sustainable sea ranching development and coastal restoration.

人工鱼礁在海洋生境恢复中发挥着重要作用；然而，它们对微生物群落的影响仍然知之甚少。本研究通过18S rDNA测序和共现网络分析，研究了海州湾浮游生物和小型动物群落（以下简称微生物群落）的季节和生境驱动变化。在春季和秋季采集AR表面和邻近海水样品，同时检测温度、盐度、深度等环境参数。结果显示α-多样性差异显著。秋季底水丰富度最高，AR面Shannon指数降低（P < 0.001）。β-多样性分析显示，ar和水柱之间存在明显的群落结构（P < 0.001），这主要是由温度和盐度的季节变化驱动的。珊瑚礁表面支持复杂的网络与>； 80%的正相关性，表明相互作用。垂直分层导致深度相关的特化，春季较深层的环节动物丰度增加（P = 0.012）。线性判别分析效应大小确定了生境特异性生物标志物，如秋季ARs中的刺胞菌和泉水中的纤毛虫，表明生态位划分。冗余分析显示盐度和温度是主要的环境驱动因素。综上所述，ar通过生境异质性和模块化网络增强了生物多样性和生态系统稳定性，缓冲了环境变化。这些发现为优化AR设计和制定支持可持续海洋牧场发展和海岸恢复的战略提供了实用见解。

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引用次数: 0

Impact assessment of land use change scenarios on landscape fragmentation and river flow discharge using SWAT model 基于SWAT模型的土地利用变化情景对景观破碎化和河流流量的影响评价

IF 2.2 4区环境科学与生态学 Q2 ECOLOGY

Ecohydrology & Hydrobiology

Pub Date : 2025-12-01 DOI: 10.1016/j.ecohyd.2025.100655

Sonia Mehri , Hamid Reza Moradi , Raoof Mostafazadeh

Landscape fragmentation is a significant ecological challenge that impacts the watershed hydrological response. This research aimed to examine the river flow response to changes in landscape metrics through historical changes and land use management scenarios. The flow response was simulated using the SWAT model and the SUFI2 program in the SWAT-CUP software package on a monthly time step. The model results were evaluated using the R2 and NS statistical coefficients, which were 0.59 and 0.54 during the calibration period, and 0.59 and 0.53 during the validation period, respectively, which proved the model's accuracy. To investigate the land use fragmentation, landscape metrics (edge density, land cover percentage, Shannon's diversity index, and landscape connectivity) were calculated using Fragstats 4.2. Additionally, the effects of land use change scenarios including increased agricultural land by 10 % (degradation), and converting moderate rangelands to good rangelands (restoration) have been assessed. The results showed an increased fragmentation, complex shapes, lower connectivity, and increasing trend of land use diversity in the watershed. The results indicate that Scenario 2, which involves the expansion of agricultural land, led to an increase in runoff at the watershed outlet. Conversely, Scenario 1, focusing on rangeland restoration, resulted in a significant reduction in runoff. Therefore, preserving the natural land uses and restoration of rangelands is one of the fundamental solutions for reducing surface runoff and the studied watershed. The findings revealed the impact of landscape change on river flow response and the contribution of river flow discharge.

景观破碎化是影响流域水文响应的重大生态挑战。本研究旨在通过历史变化和土地利用管理情景考察河流流量对景观指标变化的响应。利用SWAT- cup软件包中的SUFI2程序和SWAT模型模拟了每月时间步长的流动响应。采用R2和NS统计系数对模型结果进行评价，校正期R2和NS统计系数分别为0.59和0.54，验证期R2和NS统计系数分别为0.59和0.53，证明了模型的准确性。为了研究土地利用破碎化，使用Fragstats 4.2计算景观指标（边缘密度、土地覆盖百分比、Shannon多样性指数和景观连通性）。此外，还评估了土地利用变化情景的影响，包括农业用地增加10%（退化）和将中度牧场转变为良好牧场（恢复）。结果表明：流域破碎化程度增加，形状复杂，连通性降低，土地利用多样性呈增加趋势；结果表明，在农业用地扩张的情景2中，流域出水口径流量增加；相反，侧重于牧场恢复的情景1导致径流显著减少。因此，保护自然土地利用和恢复草地是减少地表径流和研究流域的根本解决方案之一。研究结果揭示了景观变化对河流流量响应的影响以及河流流量的贡献。

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引用次数: 0

Which is more important for stream caddisfly colonization: Substrate heterogeneity or food resources? 基质异质性和食物资源，哪个对流蝇定植更重要？

IF 2.2 4区环境科学与生态学 Q2 ECOLOGY

Ecohydrology & Hydrobiology

Pub Date : 2025-12-01 DOI: 10.1016/j.ecohyd.2025.100657

Mariana Nunes Menegat , Rozane Maria Restello , Silvia Vendruscolo Milesi , Luiz Ubiratan Hepp

Context

Significant knowledge gaps remain regarding how substrate heterogeneity and the interaction between autochthonous resources (i.e., periphyton) and allochthonous resources (i.e., particulate organic matter) shape the taxonomic and functional structure of Trichoptera assemblages.

Aims

Our objective was to verify the importance of substrate heterogeneity e availability of food resources for the functional structuring of Trichoptera assemblages in a Subtropical stream.

Methods

We used artificial samplers made of metamorphic stone to represent heterogeneous and homogeneous substrates. We collected aquatic insects, periphyton (autochthonous resource), and organic matter (allochthonous resource) over 30 days of colonization. We evaluated the relationship between chlorophyll a, organic matter, substrate, and time with each metric: abundance, richness, functional richness, and functional diversity.

Key results

Our results showed that both factors are important, but food resources had greater impact on Trichoptera colonization. Substrate heterogeneity impacted taxonomic composition, while food resources (particularly the amount of organic matter) determined functional diversity.

Conclusion

Considering that resources and substrates are associated, we highlight the need to evaluate taxonomic and functional approaches together to elucidate factors that control colonization dynamics and shape Trichoptera assemblages.

背景关于底物异质性和本地资源（即外生植物）与外来资源（即颗粒有机质）之间的相互作用如何塑造毛翅目组合的分类和功能结构，目前仍存在重大的知识空白。目的验证基质异质性和食物资源可得性对亚热带河流中毛翅目群落功能结构的重要性。方法用变质石制成的人工取样器分别代表异质和均质基质。我们采集了30天的水生昆虫、外生植物（本地资源）和有机物（外来资源）。我们用丰度、丰富度、功能丰富度和功能多样性等指标评估了叶绿素a、有机质、底物和时间之间的关系。结果表明，这两个因素都很重要，但食物资源对毛翅虫定殖的影响更大。基质异质性影响分类组成，而食物资源（特别是有机质的数量）决定功能多样性。结论考虑到资源和底物是相互关联的，我们强调有必要将分类和功能方法结合起来，以阐明控制定殖动态和形成毛翅目组合的因素。

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引用次数: 0

Improving reservoir water quality by optimizing weir operations with reinforcement learning and SWAT 通过强化学习和SWAT优化堰作业，改善水库水质

IF 2.2 4区环境科学与生态学 Q2 ECOLOGY

Ecohydrology & Hydrobiology

Pub Date : 2025-12-01 DOI: 10.1016/j.ecohyd.2025.100679

JongCheol Pyo , Sang-Soo Baek , Ather Abbas , Hyo Gyeom Kim , Jiwan Lee , Seongjoon Kim , Jong Ahn Chun , Kyung Hwa Cho

Harmful algal blooms (HABs) pose a serious threat to aquatic life in surface water. Several early warning systems have been developed to mitigate problems related to HABs by predicting HABs using machine learning methods. However, HABs are dynamic phenomena that depend on interactions between weather conditions, hydrodynamic parameters, and weir operations. This requires a real-time control strategy that uses hydrodynamic parameters to determine an optimal strategy for weir operation. In this study, reinforcement learning (RL) was employed to determine the optimum weir operation for mitigating the occurrence of HABs in six weirs of the Nakdong River, Republic of Korea. The impact of weir operation on HABs was simulated using the Soil and Water Assessment Tool (SWAT), and the outflow from the reservoir was predicted using RL agents. The RL agents were trained to predict the outflow strategy from the reservoir by minimizing the chlorophyll-a (Chl-a) concentration. Our results showed that the trained RL agents could reduce the Chl-a concentration by manipulating weir operations. The trained agents were especially effective in minimizing the peak Chl-a concentrations during summer. They reduced concentrations by an average of 35 % at six weir stations. Our study demonstrates the successful integration of RL with SWAT to improve surface water quality. The developed model has the potential to be employed as a tool for managing weir operations and water resources.

有害藻华（HABs）对地表水中的水生生物构成严重威胁。一些早期预警系统已经开发出来，通过使用机器学习方法预测赤潮来缓解与赤潮有关的问题。然而，赤潮是一种动态现象，取决于天气条件、水动力参数和堰作业之间的相互作用。这就需要一种实时控制策略，利用水动力参数来确定堰的最佳运行策略。在本研究中，采用强化学习（RL）方法确定了韩国洛东江6个堰的最佳运行方式，以减少赤潮的发生。利用水土评价工具（SWAT）模拟了水库运行对赤潮的影响，并利用RL预测了水库出水量。训练RL代理通过最小化叶绿素-a （Chl-a）浓度来预测水库的流出策略。我们的研究结果表明，训练后的RL试剂可以通过操纵堰操作来降低Chl-a的浓度。经过训练的药剂在降低夏季Chl-a浓度峰值方面特别有效。他们将六个堰站的浓度平均降低了35%。我们的研究证明了RL与SWAT的成功结合可以改善地表水的质量。开发的模型有可能被用作管理堰作业和水资源的工具。

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引用次数: 0

Storm water management model simulations of the non-point source pollution load in the northern Anhui plain 皖北平原非点源污染负荷暴雨水管理模式模拟

IF 2.2 4区环境科学与生态学 Q2 ECOLOGY

Ecohydrology & Hydrobiology

Pub Date : 2025-12-01 DOI: 10.1016/j.ecohyd.2025.100663

Yun Wan , Mingjun Li , Nan Zhang , Mengwan Lin , Yuxuan Li , Peiqing Huang , Yuqi Feng , Bai Sun , Wei Wei , Shuguang Zhu

Recently, the use of computer models to quantitatively simulate non-point source pollution has become a common and effective tool. Strengthening the research and application of non-point source pollution models is crucial for its prediction and control. Therefore, a storm water management model (SWMM) was created to simulate surface production convergence, pipe network transmissions, pollutant accumulation, and the effect of low-impact development (LID) measures. The results indicate that the total non-point source pollution load in the study area was positively related to rainfall intensity. Under different return periods, the trend of the concentration of each pollutant entering the river with time is the same. The emission of pollutants is mainly concentrated in the first 20–80 min and reaches the peak at about 60 min. Commercial and residential areas emitted far more pollutants than green spaces, the trend of the concentration of each pollutant entering the river with the change of rainfall time is the same in the three types of land use, which reaches the peak at about 55 min. Taking the S30 sub-catchment area in the study area as an example, the non-point source pollution storage tank, single LID and combined LID measures were added to the sub-catchment area, and the reduction of COD, TN, TP and SS in the sub-catchment area was compared under the condition of 0.5-year rainfall. The results show that the reduction rate of the four pollutants in the sub-catchment area by the single non-point source pollution storage tank is within 22 %; the reduction rate of four pollutants in the sub-catchment area by LID measures alone is between 24 % and 27 %, and bioretention > permeable pavement > green roof; the combined LID measures have a reduction rate of >30 % for the four pollutants in the sub-catchment area. In summary, the control effects of the three schemes are combined LID measures > single LID measures > non-point source pollution storage tanks.

近年来，利用计算机模型对非点源污染进行定量模拟已成为一种常用而有效的工具。加强非点源污染模型的研究和应用对非点源污染的预测和控制至关重要。因此，创建了一个雨水管理模型（SWMM）来模拟地表生产汇聚、管网传输、污染物积累以及低影响开发（LID）措施的效果。结果表明：研究区总非点源污染负荷与降雨强度呈正相关；在不同回归期下，各污染物进入河流的浓度随时间的变化趋势是相同的。污染物的排放主要集中在前20-80分钟，60分钟左右达到峰值。商业和住区的污染物排放量远高于绿地，三种土地利用类型进入河流的各污染物浓度随降雨时间变化的趋势相同，在55 min左右达到峰值。以研究区S30分集水区为例，在分集水区增加非点源污染储罐、单一LID和联合LID措施，比较0.5年降水条件下分集水区COD、TN、TP和SS的减少情况。结果表明：单非点源污染储罐对分集区4种污染物的减量率在22%以内；单采用LID措施，分集水区四种污染物的减量率在24% ~ 27%之间，生物滞留透水路面绿色屋顶；综合LID措施对子集水区四种污染物的减排率达30%。综上所述，三种方案的控制效果为：综合LID措施>；单一LID措施>；非点源污染储罐。

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引用次数: 0

Biological removal of bisphenol A using cross-linked aggregates of versatile peroxidase (CLEAs-VP/Ba) produced by Bjerkandera adusta CCBAS 930 Bjerkandera adusta CCBAS 930生产的多功能过氧化物酶（CLEAs-VP/Ba）交联聚集体生物去除双酚A

IF 2.2 4区环境科学与生态学 Q2 ECOLOGY

Ecohydrology & Hydrobiology

Pub Date : 2025-12-01 DOI: 10.1016/j.ecohyd.2025.100669

Kamila Rybczyńska-Tkaczyk

The aim of this study was to evaluate the feasibility of using cross-linked aggregates of versatile peroxidase (CLEAs-VP/Ba) derived from Bjerkandera adusta strain CCBAS 930 for bisphenol A (BPA) biotransformation. To optimize VP/Ba biosynthesis in stationary liquid cultures (28 °C), BPA (40 mg/mL) was added, and oxidation efficiency of 10 mM 2,6-dimethoxyphenol (2,6-DMP) was measured at various intervals (days 3, 7, 10, 14, 18, 20, and 26). The tests were conducted in 0.1 M acetate buffer at pH 3.0 and 5.0, with and without Mn²⁺. Maximum activity for VP/Ba was determined on day 14, after which the culture fluid was separated from the mycelium and ammonium sulfate (80 %) and 72 mM glutaraldehyde was added to prepare CLEAs-VP/Ba (22 h, 150 rpm, 30 °C). The activity recovery (AR_CLEAs-VP/Ba) and aggregation yield (AY_CLEAs-VP/Ba) were 11.65 % and 48.95 %, respectively. The BPA (10 mg/L) removal efficiency of CLEAs-VP/Ba and 10 mM H₂O₂ was measured spectrophotometrically after 30, 60, 90 and 120 min. Results showed that CLEAs significantly increased the activity of VP/Ba peroxidase, achieving an 83 % BPA removal efficiency after 120 min (4.15 mg/h). Moreover, CLEAs-VP/Ba-treated samples exhibited reduced biotoxicity, with no observed phytotoxicity. The treatment also mitigated long-term adverse effects, such as genotoxicity. In case of estrogenic activity, no significant decrease was observed after the use of CLEAs-VP/Ba.

本研究的目的是评估利用Bjerkandera adusta菌株CCBAS 930衍生的多功能过氧化物酶（CLEAs-VP/Ba）交联聚集体进行双酚A （BPA）生物转化的可行性。为了优化固定液体培养（28°C）中VP/Ba的生物合成，添加BPA (40 mg/mL)，并在不同的时间间隔（第3、7、10、14、18、20和26天）测量10 mM 2,6-二甲氧基苯酚（2,6- dmp）的氧化效率。实验在0.1 M醋酸缓冲液中进行，pH为3.0和5.0，含和不含Mn2+。在第14天测定VP/Ba的最大活性，之后将培养液与菌丝和硫酸铵（80%）分离，加入72 mM戊二醛，制备CLEAs-VP/Ba （22 h, 150 rpm, 30°C）。活性恢复（ARCLEAs-VP/Ba）和聚合率（AYCLEAs-VP/Ba）分别为11.65%和48.95%。分别在30、60、90和120 min后测定CLEAs-VP/Ba和10 mM H2O2对BPA （10 mg/L）的去除率。结果表明，CLEAs显著提高了VP/Ba过氧化物酶的活性，在120 min （4.15 mg/h）后，BPA的去除率达到83%。此外，CLEAs-VP/ ba处理的样品显示出降低的生物毒性，没有观察到植物毒性。该疗法还减轻了遗传毒性等长期不良反应。在雌激素活性方面，使用CLEAs-VP/Ba后未观察到明显降低。

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引用次数: 0