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Hydrodynamic modeling as a tool for analyzing the hydrological effects of river maintenance activities 水动力模拟作为分析河流养护活动水文效应的工具
IF 2.2 4区 环境科学与生态学 Q2 ECOLOGY Pub Date : 2025-12-01 DOI: 10.1016/j.ecohyd.2025.100677
Maria Grodzka-Łukaszewska , Dorota Mirosław-Świątek , Mateusz Grygoruk , Tomasz Krepski , Sylwia Szporak-Wasilewska , Grzegorz Sinicyn , Tomasz Okruszko
Climate change exacerbates water scarcity globally, impacting vital wetland ecosystems that provide crucial ecological services. As a result, peatland areas experience a gradual decrease in water table levels, leading to their degradation and emphasizing the need for effective water level management strategies. This study examines the hydrological and ecological consequences of riverbed maintenance—specifically vegetation mowing—on the Upper Biebrza Valley. Using a coupled hydrodynamic-groundwater flow model supported by an Artificial Neural Network (ANN), we simulated the impacts of different mowing scenarios on groundwater levels, water balance, and vegetation response.
The results show that mowing vegetation from the riverbed temporarily lowers river water levels, which increases the hydraulic gradient and accelerates water outflow from adjacent peatlands. In the most intensive scenario, riverbed mowing led to a short-term increase of up to 24 % in groundwater discharge to the river compared to the reference (non-mowed) condition. However, this effect is temporally limited and site-specific. The impact on groundwater levels and wetland habitats varied significantly between plant communities, with species located closest to the river showing the highest sensitivity to water table decline.
These findings demonstrate that routine riverbed maintenance can negatively affect peatland hydrology and ecosystem resilience, even when applied partially. The study emphasizes the need to reassess standard maintenance practices, particularly in protected and climate-sensitive landscapes.
气候变化加剧了全球水资源短缺,影响了提供重要生态服务的重要湿地生态系统。因此,泥炭地地区的地下水位逐渐下降,导致其退化,并强调需要有效的水位管理战略。本研究考察了比布尔扎上游河谷河床维护(特别是植被修剪)的水文和生态后果。利用人工神经网络(ANN)支持的水动力-地下水耦合模型,模拟了不同刈割情景对地下水位、水分平衡和植被响应的影响。结果表明:刈割植被使河床暂时降低了水位,增加了水力梯度,加速了邻近泥炭地的水流;在最密集的情况下,与参考(未刈割)条件相比,河床刈割导致短期内向河流排放的地下水增加了24%。然而,这种影响是暂时有限的,而且是局部的。不同植物群落对地下水位和湿地生境的影响差异显著,靠近河流的物种对地下水位下降的敏感性最高。这些发现表明,常规的河床维护会对泥炭地水文和生态系统恢复力产生负面影响,即使是部分实施。该研究强调有必要重新评估标准的维护做法,特别是在受保护和气候敏感的景观中。
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引用次数: 0
Polystyrene microbeads in freshwater ecosystems - Ecotoxicological effects on Daphnia magna 淡水生态系统中的聚苯乙烯微珠。对大水蚤的生态毒理学影响
IF 2.2 4区 环境科学与生态学 Q2 ECOLOGY Pub Date : 2025-12-01 DOI: 10.1016/j.ecohyd.2025.100647
Nina Doskocz , Dominika Skwarska , Katarzyna Affek , Marta Kucharska , Jessica Hua , Monika Załęska-Radziwiłł
The global surge in plastic production has led to widespread contamination of aquatic ecosystems with microplastics. Despite increasing concerns, data on their impact on freshwater species are limited, which hampers comprehensive ecological risk assessments.
This study investigates the effects of polystyrene microparticles on the survival and physiological processes of the freshwater crustacean Daphnia magna. The research evaluates the direct effects of microplastics in acute and chronic exposures, correlating impacts with particle size and concentration, and identifying the locations of microplastic accumulation in the organisms.
Results show that polystyrene microplastics have size- and concentration-dependent negative effects on D. magna, causing particle accumulation in the digestive system, cellular damage, reproductive inhibition, and immobilization. The most toxic particles were 1 μm in size, while 0.5 μm and 3 μm particles exhibited lower acute toxicity. Chronic exposure led to reduction in offspring body length across most microbead sizes. The most pronounced effects were observed for 3 µm particles in terms of the number of neonates, and for 1 µm particles in terms of body length. Transmission electron microscopy revealed that the smallest particles (0.5 μm and 0.75 μm) most effectively penetrated intestinal cells, causing cytoplasmic accumulation, mitochondrial damage, and cell disruption. These findings contribute to improving environmental risk assessments for microplastics in freshwater ecosystems.
全球塑料生产的激增导致水生生态系统受到微塑料的广泛污染。尽管人们越来越关注,但关于它们对淡水物种影响的数据有限,这妨碍了全面的生态风险评估。本研究探讨了聚苯乙烯微粒对淡水甲壳类大水蚤(Daphnia magna)生存和生理过程的影响。该研究评估了微塑料在急性和慢性暴露中的直接影响,将影响与颗粒大小和浓度联系起来,并确定了微塑料在生物体中积聚的位置。结果表明,聚苯乙烯微塑料对D. magna具有粒径和浓度依赖的负面影响,可引起消化系统的颗粒积聚、细胞损伤、生殖抑制和固定化。粒径为1 μm的颗粒毒性最大,0.5 μm和3 μm的颗粒毒性较小。长期暴露导致大多数微珠大小的后代体长减少。3µm颗粒对新生儿数量的影响最为显著,1µm颗粒对体长的影响最为显著。透射电镜显示,最小的颗粒(0.5 μm和0.75 μm)最有效地穿透肠细胞,引起细胞质积聚,线粒体损伤和细胞破坏。这些发现有助于改进淡水生态系统中微塑料的环境风险评估。
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引用次数: 0
Seasonal shifts and habitat complexity: unveiling plankton-meiofauna communities dynamics in artificial reef ecosystems of Haizhou Bay, China 季节变化与生境复杂性:揭示海州湾人工鱼礁生态系统浮游生物群落动态
IF 2.2 4区 环境科学与生态学 Q2 ECOLOGY Pub Date : 2025-12-01 DOI: 10.1016/j.ecohyd.2025.100668
Ze Zhao , Jikun Lu , Tianhang Jia , Guanghui Fu , Miaomiao Sun , Shuo Zhang , Shike Gao
Artificial reefs (ARs) play a vital role in marine habitat restoration; however, their influence on microbial communities remains poorly understood. In the present study, we investigated seasonal- and habitat-driven variations in plankton and meiofauna communities (herein referred to as microbial communities) in Haizhou Bay, China, by performing 18S rDNA sequencing and co-occurrence network analysis. Samples were collected from AR surfaces and adjacent seawater in spring and autumn, and the environmental parameters, such as temperature, salinity, and depth, were concurrently detected. The results revealed significant α-diversity differences. Autumn bottom waters exhibited the highest richness, whereas AR surfaces exhibited decreased Shannon indices (P < 0.001). β-Diversity analysis revealed distinct community structures between ARs and water columns (P < 0.001), which were largely driven by seasonal shifts in temperature and salinity. Reef surfaces supported complex networks with >80 % positive correlations, suggesting mutualistic interactions. Vertical stratification on ARs led to depth-dependent specialization, with increasing Annelida abundance at deeper layers in spring (P = 0.012). Linear discriminant analysis effect size identified habitat-specific biomarkers, such as Cnidaria in autumn ARs and Ciliophora in spring water, suggesting niche partitioning. Redundancy analysis revealed salinity and temperature as major environmental drivers. Altogether, our findings suggest that ARs enhance biodiversity and ecosystem stability through habitat heterogeneity and modular networks, buffering environmental changes. These findings offer practical insights for optimizing AR designs and formulating strategies to support sustainable sea ranching development and coastal restoration.
人工鱼礁在海洋生境恢复中发挥着重要作用;然而,它们对微生物群落的影响仍然知之甚少。本研究通过18S rDNA测序和共现网络分析,研究了海州湾浮游生物和小型动物群落(以下简称微生物群落)的季节和生境驱动变化。在春季和秋季采集AR表面和邻近海水样品,同时检测温度、盐度、深度等环境参数。结果显示α-多样性差异显著。秋季底水丰富度最高,AR面Shannon指数降低(P < 0.001)。β-多样性分析显示,ar和水柱之间存在明显的群落结构(P < 0.001),这主要是由温度和盐度的季节变化驱动的。珊瑚礁表面支持复杂的网络与>; 80%的正相关性,表明相互作用。垂直分层导致深度相关的特化,春季较深层的环节动物丰度增加(P = 0.012)。线性判别分析效应大小确定了生境特异性生物标志物,如秋季ARs中的刺胞菌和泉水中的纤毛虫,表明生态位划分。冗余分析显示盐度和温度是主要的环境驱动因素。综上所述,ar通过生境异质性和模块化网络增强了生物多样性和生态系统稳定性,缓冲了环境变化。这些发现为优化AR设计和制定支持可持续海洋牧场发展和海岸恢复的战略提供了实用见解。
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引用次数: 0
Impact assessment of land use change scenarios on landscape fragmentation and river flow discharge using SWAT model 基于SWAT模型的土地利用变化情景对景观破碎化和河流流量的影响评价
IF 2.2 4区 环境科学与生态学 Q2 ECOLOGY Pub Date : 2025-12-01 DOI: 10.1016/j.ecohyd.2025.100655
Sonia Mehri , Hamid Reza Moradi , Raoof Mostafazadeh
Landscape fragmentation is a significant ecological challenge that impacts the watershed hydrological response. This research aimed to examine the river flow response to changes in landscape metrics through historical changes and land use management scenarios. The flow response was simulated using the SWAT model and the SUFI2 program in the SWAT-CUP software package on a monthly time step. The model results were evaluated using the R2 and NS statistical coefficients, which were 0.59 and 0.54 during the calibration period, and 0.59 and 0.53 during the validation period, respectively, which proved the model's accuracy. To investigate the land use fragmentation, landscape metrics (edge density, land cover percentage, Shannon's diversity index, and landscape connectivity) were calculated using Fragstats 4.2. Additionally, the effects of land use change scenarios including increased agricultural land by 10 % (degradation), and converting moderate rangelands to good rangelands (restoration) have been assessed. The results showed an increased fragmentation, complex shapes, lower connectivity, and increasing trend of land use diversity in the watershed. The results indicate that Scenario 2, which involves the expansion of agricultural land, led to an increase in runoff at the watershed outlet. Conversely, Scenario 1, focusing on rangeland restoration, resulted in a significant reduction in runoff. Therefore, preserving the natural land uses and restoration of rangelands is one of the fundamental solutions for reducing surface runoff and the studied watershed. The findings revealed the impact of landscape change on river flow response and the contribution of river flow discharge.
景观破碎化是影响流域水文响应的重大生态挑战。本研究旨在通过历史变化和土地利用管理情景考察河流流量对景观指标变化的响应。利用SWAT- cup软件包中的SUFI2程序和SWAT模型模拟了每月时间步长的流动响应。采用R2和NS统计系数对模型结果进行评价,校正期R2和NS统计系数分别为0.59和0.54,验证期R2和NS统计系数分别为0.59和0.53,证明了模型的准确性。为了研究土地利用破碎化,使用Fragstats 4.2计算景观指标(边缘密度、土地覆盖百分比、Shannon多样性指数和景观连通性)。此外,还评估了土地利用变化情景的影响,包括农业用地增加10%(退化)和将中度牧场转变为良好牧场(恢复)。结果表明:流域破碎化程度增加,形状复杂,连通性降低,土地利用多样性呈增加趋势;结果表明,在农业用地扩张的情景2中,流域出水口径流量增加;相反,侧重于牧场恢复的情景1导致径流显著减少。因此,保护自然土地利用和恢复草地是减少地表径流和研究流域的根本解决方案之一。研究结果揭示了景观变化对河流流量响应的影响以及河流流量的贡献。
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引用次数: 0
Which is more important for stream caddisfly colonization: Substrate heterogeneity or food resources? 基质异质性和食物资源,哪个对流蝇定植更重要?
IF 2.2 4区 环境科学与生态学 Q2 ECOLOGY Pub Date : 2025-12-01 DOI: 10.1016/j.ecohyd.2025.100657
Mariana Nunes Menegat , Rozane Maria Restello , Silvia Vendruscolo Milesi , Luiz Ubiratan Hepp

Context

Significant knowledge gaps remain regarding how substrate heterogeneity and the interaction between autochthonous resources (i.e., periphyton) and allochthonous resources (i.e., particulate organic matter) shape the taxonomic and functional structure of Trichoptera assemblages.

Aims

Our objective was to verify the importance of substrate heterogeneity e availability of food resources for the functional structuring of Trichoptera assemblages in a Subtropical stream.

Methods

We used artificial samplers made of metamorphic stone to represent heterogeneous and homogeneous substrates. We collected aquatic insects, periphyton (autochthonous resource), and organic matter (allochthonous resource) over 30 days of colonization. We evaluated the relationship between chlorophyll a, organic matter, substrate, and time with each metric: abundance, richness, functional richness, and functional diversity.

Key results

Our results showed that both factors are important, but food resources had greater impact on Trichoptera colonization. Substrate heterogeneity impacted taxonomic composition, while food resources (particularly the amount of organic matter) determined functional diversity.

Conclusion

Considering that resources and substrates are associated, we highlight the need to evaluate taxonomic and functional approaches together to elucidate factors that control colonization dynamics and shape Trichoptera assemblages.
背景关于底物异质性和本地资源(即外生植物)与外来资源(即颗粒有机质)之间的相互作用如何塑造毛翅目组合的分类和功能结构,目前仍存在重大的知识空白。目的验证基质异质性和食物资源可得性对亚热带河流中毛翅目群落功能结构的重要性。方法用变质石制成的人工取样器分别代表异质和均质基质。我们采集了30天的水生昆虫、外生植物(本地资源)和有机物(外来资源)。我们用丰度、丰富度、功能丰富度和功能多样性等指标评估了叶绿素a、有机质、底物和时间之间的关系。结果表明,这两个因素都很重要,但食物资源对毛翅虫定殖的影响更大。基质异质性影响分类组成,而食物资源(特别是有机质的数量)决定功能多样性。结论考虑到资源和底物是相互关联的,我们强调有必要将分类和功能方法结合起来,以阐明控制定殖动态和形成毛翅目组合的因素。
{"title":"Which is more important for stream caddisfly colonization: Substrate heterogeneity or food resources?","authors":"Mariana Nunes Menegat ,&nbsp;Rozane Maria Restello ,&nbsp;Silvia Vendruscolo Milesi ,&nbsp;Luiz Ubiratan Hepp","doi":"10.1016/j.ecohyd.2025.100657","DOIUrl":"10.1016/j.ecohyd.2025.100657","url":null,"abstract":"<div><h3>Context</h3><div>Significant knowledge gaps remain regarding how substrate heterogeneity and the interaction between autochthonous resources (i.e., periphyton) and allochthonous resources (i.e., particulate organic matter) shape the taxonomic and functional structure of Trichoptera assemblages.</div></div><div><h3>Aims</h3><div>Our objective was to verify the importance of substrate heterogeneity e availability of food resources for the functional structuring of Trichoptera assemblages in a Subtropical stream.</div></div><div><h3>Methods</h3><div><span>We used artificial samplers made of metamorphic stone to represent heterogeneous and homogeneous substrates. We collected aquatic insects, periphyton (autochthonous resource), and organic matter (allochthonous resource) over 30 days of colonization. We evaluated the relationship between chlorophyll </span><em>a</em><span>, organic matter, substrate, and time with each metric: abundance, richness, functional richness, and functional diversity.</span></div></div><div><h3>Key results</h3><div>Our results showed that both factors are important, but food resources had greater impact on Trichoptera colonization. Substrate heterogeneity impacted taxonomic composition, while food resources (particularly the amount of organic matter) determined functional diversity.</div></div><div><h3>Conclusion</h3><div>Considering that resources and substrates are associated, we highlight the need to evaluate taxonomic and functional approaches together to elucidate factors that control colonization dynamics and shape Trichoptera assemblages.</div></div>","PeriodicalId":56070,"journal":{"name":"Ecohydrology & Hydrobiology","volume":"25 4","pages":"Article 100657"},"PeriodicalIF":2.2,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145739218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Improving reservoir water quality by optimizing weir operations with reinforcement learning and SWAT 通过强化学习和SWAT优化堰作业,改善水库水质
IF 2.2 4区 环境科学与生态学 Q2 ECOLOGY Pub Date : 2025-12-01 DOI: 10.1016/j.ecohyd.2025.100679
JongCheol Pyo , Sang-Soo Baek , Ather Abbas , Hyo Gyeom Kim , Jiwan Lee , Seongjoon Kim , Jong Ahn Chun , Kyung Hwa Cho
Harmful algal blooms (HABs) pose a serious threat to aquatic life in surface water. Several early warning systems have been developed to mitigate problems related to HABs by predicting HABs using machine learning methods. However, HABs are dynamic phenomena that depend on interactions between weather conditions, hydrodynamic parameters, and weir operations. This requires a real-time control strategy that uses hydrodynamic parameters to determine an optimal strategy for weir operation. In this study, reinforcement learning (RL) was employed to determine the optimum weir operation for mitigating the occurrence of HABs in six weirs of the Nakdong River, Republic of Korea. The impact of weir operation on HABs was simulated using the Soil and Water Assessment Tool (SWAT), and the outflow from the reservoir was predicted using RL agents. The RL agents were trained to predict the outflow strategy from the reservoir by minimizing the chlorophyll-a (Chl-a) concentration. Our results showed that the trained RL agents could reduce the Chl-a concentration by manipulating weir operations. The trained agents were especially effective in minimizing the peak Chl-a concentrations during summer. They reduced concentrations by an average of 35 % at six weir stations. Our study demonstrates the successful integration of RL with SWAT to improve surface water quality. The developed model has the potential to be employed as a tool for managing weir operations and water resources.
有害藻华(HABs)对地表水中的水生生物构成严重威胁。一些早期预警系统已经开发出来,通过使用机器学习方法预测赤潮来缓解与赤潮有关的问题。然而,赤潮是一种动态现象,取决于天气条件、水动力参数和堰作业之间的相互作用。这就需要一种实时控制策略,利用水动力参数来确定堰的最佳运行策略。在本研究中,采用强化学习(RL)方法确定了韩国洛东江6个堰的最佳运行方式,以减少赤潮的发生。利用水土评价工具(SWAT)模拟了水库运行对赤潮的影响,并利用RL预测了水库出水量。训练RL代理通过最小化叶绿素-a (Chl-a)浓度来预测水库的流出策略。我们的研究结果表明,训练后的RL试剂可以通过操纵堰操作来降低Chl-a的浓度。经过训练的药剂在降低夏季Chl-a浓度峰值方面特别有效。他们将六个堰站的浓度平均降低了35%。我们的研究证明了RL与SWAT的成功结合可以改善地表水的质量。开发的模型有可能被用作管理堰作业和水资源的工具。
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引用次数: 0
Storm water management model simulations of the non-point source pollution load in the northern Anhui plain 皖北平原非点源污染负荷暴雨水管理模式模拟
IF 2.2 4区 环境科学与生态学 Q2 ECOLOGY Pub Date : 2025-12-01 DOI: 10.1016/j.ecohyd.2025.100663
Yun Wan , Mingjun Li , Nan Zhang , Mengwan Lin , Yuxuan Li , Peiqing Huang , Yuqi Feng , Bai Sun , Wei Wei , Shuguang Zhu
Recently, the use of computer models to quantitatively simulate non-point source pollution has become a common and effective tool. Strengthening the research and application of non-point source pollution models is crucial for its prediction and control. Therefore, a storm water management model (SWMM) was created to simulate surface production convergence, pipe network transmissions, pollutant accumulation, and the effect of low-impact development (LID) measures. The results indicate that the total non-point source pollution load in the study area was positively related to rainfall intensity. Under different return periods, the trend of the concentration of each pollutant entering the river with time is the same. The emission of pollutants is mainly concentrated in the first 20–80 min and reaches the peak at about 60 min. Commercial and residential areas emitted far more pollutants than green spaces, the trend of the concentration of each pollutant entering the river with the change of rainfall time is the same in the three types of land use, which reaches the peak at about 55 min. Taking the S30 sub-catchment area in the study area as an example, the non-point source pollution storage tank, single LID and combined LID measures were added to the sub-catchment area, and the reduction of COD, TN, TP and SS in the sub-catchment area was compared under the condition of 0.5-year rainfall. The results show that the reduction rate of the four pollutants in the sub-catchment area by the single non-point source pollution storage tank is within 22 %; the reduction rate of four pollutants in the sub-catchment area by LID measures alone is between 24 % and 27 %, and bioretention > permeable pavement > green roof; the combined LID measures have a reduction rate of >30 % for the four pollutants in the sub-catchment area. In summary, the control effects of the three schemes are combined LID measures > single LID measures > non-point source pollution storage tanks.
近年来,利用计算机模型对非点源污染进行定量模拟已成为一种常用而有效的工具。加强非点源污染模型的研究和应用对非点源污染的预测和控制至关重要。因此,创建了一个雨水管理模型(SWMM)来模拟地表生产汇聚、管网传输、污染物积累以及低影响开发(LID)措施的效果。结果表明:研究区总非点源污染负荷与降雨强度呈正相关;在不同回归期下,各污染物进入河流的浓度随时间的变化趋势是相同的。污染物的排放主要集中在前20-80分钟,60分钟左右达到峰值。商业和住区的污染物排放量远高于绿地,三种土地利用类型进入河流的各污染物浓度随降雨时间变化的趋势相同,在55 min左右达到峰值。以研究区S30分集水区为例,在分集水区增加非点源污染储罐、单一LID和联合LID措施,比较0.5年降水条件下分集水区COD、TN、TP和SS的减少情况。结果表明:单非点源污染储罐对分集区4种污染物的减量率在22%以内;单采用LID措施,分集水区四种污染物的减量率在24% ~ 27%之间,生物滞留透水路面绿色屋顶;综合LID措施对子集水区四种污染物的减排率达30%。综上所述,三种方案的控制效果为:综合LID措施>;单一LID措施>;非点源污染储罐。
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引用次数: 0
Biological removal of bisphenol A using cross-linked aggregates of versatile peroxidase (CLEAs-VP/Ba) produced by Bjerkandera adusta CCBAS 930 Bjerkandera adusta CCBAS 930生产的多功能过氧化物酶(CLEAs-VP/Ba)交联聚集体生物去除双酚A
IF 2.2 4区 环境科学与生态学 Q2 ECOLOGY Pub Date : 2025-12-01 DOI: 10.1016/j.ecohyd.2025.100669
Kamila Rybczyńska-Tkaczyk
The aim of this study was to evaluate the feasibility of using cross-linked aggregates of versatile peroxidase (CLEAs-VP/Ba) derived from Bjerkandera adusta strain CCBAS 930 for bisphenol A (BPA) biotransformation. To optimize VP/Ba biosynthesis in stationary liquid cultures (28 °C), BPA (40 mg/mL) was added, and oxidation efficiency of 10 mM 2,6-dimethoxyphenol (2,6-DMP) was measured at various intervals (days 3, 7, 10, 14, 18, 20, and 26). The tests were conducted in 0.1 M acetate buffer at pH 3.0 and 5.0, with and without Mn2+. Maximum activity for VP/Ba was determined on day 14, after which the culture fluid was separated from the mycelium and ammonium sulfate (80 %) and 72 mM glutaraldehyde was added to prepare CLEAs-VP/Ba (22 h, 150 rpm, 30 °C). The activity recovery (ARCLEAs-VP/Ba) and aggregation yield (AYCLEAs-VP/Ba) were 11.65 % and 48.95 %, respectively. The BPA (10 mg/L) removal efficiency of CLEAs-VP/Ba and 10 mM H2O2 was measured spectrophotometrically after 30, 60, 90 and 120 min. Results showed that CLEAs significantly increased the activity of VP/Ba peroxidase, achieving an 83 % BPA removal efficiency after 120 min (4.15 mg/h). Moreover, CLEAs-VP/Ba-treated samples exhibited reduced biotoxicity, with no observed phytotoxicity. The treatment also mitigated long-term adverse effects, such as genotoxicity. In case of estrogenic activity, no significant decrease was observed after the use of CLEAs-VP/Ba.
本研究的目的是评估利用Bjerkandera adusta菌株CCBAS 930衍生的多功能过氧化物酶(CLEAs-VP/Ba)交联聚集体进行双酚A (BPA)生物转化的可行性。为了优化固定液体培养(28°C)中VP/Ba的生物合成,添加BPA (40 mg/mL),并在不同的时间间隔(第3、7、10、14、18、20和26天)测量10 mM 2,6-二甲氧基苯酚(2,6- dmp)的氧化效率。实验在0.1 M醋酸缓冲液中进行,pH为3.0和5.0,含和不含Mn2+。在第14天测定VP/Ba的最大活性,之后将培养液与菌丝和硫酸铵(80%)分离,加入72 mM戊二醛,制备CLEAs-VP/Ba (22 h, 150 rpm, 30°C)。活性恢复(ARCLEAs-VP/Ba)和聚合率(AYCLEAs-VP/Ba)分别为11.65%和48.95%。分别在30、60、90和120 min后测定CLEAs-VP/Ba和10 mM H2O2对BPA (10 mg/L)的去除率。结果表明,CLEAs显著提高了VP/Ba过氧化物酶的活性,在120 min (4.15 mg/h)后,BPA的去除率达到83%。此外,CLEAs-VP/ ba处理的样品显示出降低的生物毒性,没有观察到植物毒性。该疗法还减轻了遗传毒性等长期不良反应。在雌激素活性方面,使用CLEAs-VP/Ba后未观察到明显降低。
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引用次数: 0
Temperature-driven nonlinear thresholds and time-lags in vegetation response to extreme climate events via machine learning: Evidence from China's Poyang Lake 基于机器学习的植被对极端气候事件响应的温度驱动非线性阈值和时滞:来自中国鄱阳湖的证据
IF 2.2 4区 环境科学与生态学 Q2 ECOLOGY Pub Date : 2025-12-01 DOI: 10.1016/j.ecohyd.2025.100685
Fangfei Zhang , Wei Yang , Dongdong Shao
Understanding how extreme climate events impact vegetation dynamics is crucial for ecosystem resilience assessment and adaptive management, but existing research has yet to fully explore the intricate mechanisms governing vegetation responses to climate extremes. Using Poyang Lake as a case study, we assess spatiotemporal variations in NDVI over periods from 2000 to 2020, and examine effects of 23 extreme climate indices (including rainfall and temperature metrics) on vegetation dynamics, considering lagged and cumulative effects. Nine temporal effect scenarios were evaluated to clarify the complex relationships between extreme climate events and vegetation responses. Our results indicate that the NDVI in the Poyang Lake region increases significantly, with a mean growth rate of 0.037 per decade (p < 0.01). Extreme temperature indices exhibited the strongest correlations with vegetation response, with peak correlation observed at one-month accumulation periods, while for most extreme precipitation indices, vegetation responses were stronger with accumulated effects over 2–3 months than with simple lag effects. Temperature-related indices were clearly dominant in the explainable models, with monthly minimum value of daily minimum temperature (TNn) being the most influential factor, accounting for approximately 23 % of the explained variance. SHapley Additive exPlanations (SHAP) value analysis revealed a pronounced non-linear threshold effect: NDVI increased sharply as TNn rose from 0 °C to 12 °C, then stabilized at higher values, which indicates that cold stress represents a major constraint on vegetation growth with a clear physiological threshold. These findings underscore critical need for a more comprehensive and explicit depiction of how climate extremes influence vegetation.
了解极端气候事件如何影响植被动态对于生态系统恢复力评估和适应性管理至关重要,但现有研究尚未充分探索极端气候对植被响应的复杂机制。本文以鄱阳湖为例,分析了2000 - 2020年鄱阳湖NDVI的时空变化特征,并分析了23个极端气候指数(包括降雨和温度指标)对植被动态的滞后效应和累积效应。为了阐明极端气候事件与植被响应之间的复杂关系,对9种时间效应情景进行了评估。结果表明,鄱阳湖地区NDVI显著增加,平均增长率为0.037 / a (p < 0.01)。极端温度指数与植被响应的相关性最强,在1个月的积累期达到峰值,而对于大多数极端降水指数,植被响应在2-3个月的累积效应强于单纯滞后效应。在可解释模型中,温度相关指数明显占主导地位,其中月最小日最低气温(TNn)是影响最大的因子,约占可解释方差的23%。SHapley加性解释(SHAP)值分析揭示了显著的非线性阈值效应,NDVI随TNn从0°C升高到12°C急剧增加,然后在较高值处趋于稳定,表明冷胁迫是植被生长的主要制约因素,具有明确的生理阈值。这些发现强调了对极端气候如何影响植被进行更全面、更明确描述的迫切需要。
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引用次数: 0
Structural and functional responses of Panicum antidotale to diverse ecohydrological conditions 不同生态水文条件下解毒草的结构和功能响应
IF 2.2 4区 环境科学与生态学 Q2 ECOLOGY Pub Date : 2025-12-01 DOI: 10.1016/j.ecohyd.2025.100686
Muhammad Irshad , Mansoor Hameed , Farooq Ahmad , Ummar Iqbal , Jazab Shafqat , Ansa Asghar
Understanding plant–water relationships is vital for managing ecosystems under escalating hydrological stress. Panicum antidotale (a C₄ perennial grass) exhibits significant phenotypic plasticity, making it a suitable model for assessing structural and functional adaptation across ecohydrological gradients. This study evaluated eight natural populations from freshwater ponds, irrigation canals, riverbanks, and sewerage channels in Punjab, Pakistan. Results revealed that irrigation canal populations (CW, KP) exhibited maximum plant height, leaf number, and leaf area, reflecting a growth-acquisitive strategy supported by high chlorophyll b and carotenoid content. Freshwater pond populations (BS, BC) also followed an acquisitive model, with elevated chlorophyll a, large bulliform cells, and moderate growth, prioritizing photosynthetic efficiency under stable conditions. In contrast, sewerage populations (SA, TSP) adopted a conservative strategy, with stunted shoot growth but enhanced root elongation, thickened sclerenchyma and epidermis, and increased levels of osmolytes (proline, glycine betaine), antioxidants (ascorbic acid, CAT, POD), and oxidative stress markers (H₂O₂, MDA)—indicating investment in defense and stress tolerance over productivity. Riverbank populations (RA, RR) displayed an intermediate strategy, with the highest shoot biomass, midrib and lamina thickness, phloem area, and stomatal density—traits that aid in gas exchange, hydraulic conductivity, and structural support in dynamic flood-prone environments. Anatomically, these populations also showed well-developed vascular systems and thickened leaf structures, contributing to both ecological persistence and hydrological functionality. These specific adaptations support resource allocation theory and position P. antidotale as a candidate for ecological restoration, erosion control, and water quality improvement.
了解植物与水的关系对于管理不断升级的水文压力下的生态系统至关重要。Panicum antidotale(一种C₄多年生草)表现出显著的表型可塑性,使其成为评估跨生态水文梯度结构和功能适应的合适模型。本研究评估了来自巴基斯坦旁遮普淡水池塘、灌溉渠、河岸和污水渠的8个自然种群。结果表明,灌渠种群(CW、KP)表现出最大的株高、叶数和叶面积,反映了高叶绿素b和类胡萝卜素含量支持的生长获取策略。淡水池塘种群(BS, BC)也遵循获取模式,在稳定的条件下,叶绿素a升高,球状细胞大,生长缓慢,优先考虑光合效率。相比之下,污水处理群体(SA、TSP)则采取保守策略,茎部生长发育迟缓,但根伸长增强,厚壁组织和表皮增厚,渗透物(脯氨酸、甘氨酸甜菜碱)、抗氧化剂(抗坏血酸、CAT、POD)和氧化应激标志物(h2o2、MDA)水平增加,表明在防御和抗逆性方面的投入高于生产力。河岸种群(RA, RR)表现出一种中间策略,在动态洪水易发环境中具有最高的茎部生物量、中脉和层厚、韧皮部面积和气孔密度特征,有助于气体交换、水力传导和结构支撑。解剖学上,这些种群也表现出发达的维管系统和加厚的叶结构,有助于生态持久性和水文功能。这些特殊的适应性支持了资源配置理论,并使解藻成为生态恢复、侵蚀控制和水质改善的候选植物。
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引用次数: 0
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Ecohydrology & Hydrobiology
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