Pub Date : 2019-02-06DOI: 10.1007/s13404-019-00252-2
Sahar Mohammadpour Lashkari, Hasan Kariminezhad, Nazanin Safarnezhad, Hossein Amani
Although antimicrobial photothermal inactivation of naked gold nanostructures using powerful pulsed lasers has been previously studied, there are little reports about their photodynamic antimicrobial properties under the irradiation of low-power density continuous wave lasers. Therefore, this paper attempts to fill this gap. In this paper, we studied the effects of a 40-mW/cm2 continuous Nd:Yag laser at 532?nm and naked gold nanoparticles on inactivation of Escherichia coli ATCC25922. According to our results, 60?min illumination using the Nd:Yag laser caused a 0.15log reduction of the bacterial viability. Also, the employed gold nanoparticles with an average size of 15?nm were toxic to E. coli ATCC 25922 in the concentrations above 0.5?μg/ml. In addition, synergistic effects of 0.5?μg/ml gold nanoparticles and the light illumination led to a 2.43log reduction of the viability after a 60-min exposure and did not show any considerable temperature change on the media. The obtained results were justified based on the possible interaction mechanisms of low-power density laser lights and naked gold nanoparticles. The paper is proposed as a prelude for future research about localized inactivation of resistant pathogens with minimum side effects on neighbor tissues.
{"title":"Surface plasmon resonance of naked gold nanoparticles for photodynamic inactivation of Escherichia coli","authors":"Sahar Mohammadpour Lashkari, Hasan Kariminezhad, Nazanin Safarnezhad, Hossein Amani","doi":"10.1007/s13404-019-00252-2","DOIUrl":"https://doi.org/10.1007/s13404-019-00252-2","url":null,"abstract":"<p>Although antimicrobial photothermal inactivation of naked gold nanostructures using powerful pulsed lasers has been previously studied, there are little reports about their photodynamic antimicrobial properties under the irradiation of low-power density continuous wave lasers. Therefore, this paper attempts to fill this gap. In this paper, we studied the effects of a 40-mW/cm<sup>2</sup> continuous Nd:Yag laser at 532?nm and naked gold nanoparticles on inactivation of <i>Escherichia coli</i> ATCC25922. According to our results, 60?min illumination using the Nd:Yag laser caused a 0.15log reduction of the bacterial viability. Also, the employed gold nanoparticles with an average size of 15?nm were toxic to <i>E. coli</i> ATCC 25922 in the concentrations above 0.5?μg/ml. In addition, synergistic effects of 0.5?μg/ml gold nanoparticles and the light illumination led to a 2.43log reduction of the viability after a 60-min exposure and did not show any considerable temperature change on the media. The obtained results were justified based on the possible interaction mechanisms of low-power density laser lights and naked gold nanoparticles. The paper is proposed as a prelude for future research about localized inactivation of resistant pathogens with minimum side effects on neighbor tissues.</p>","PeriodicalId":581,"journal":{"name":"Gold Bulletin","volume":"52 1","pages":"51 - 60"},"PeriodicalIF":2.2,"publicationDate":"2019-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s13404-019-00252-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4244528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-03DOI: 10.1007/s13404-018-00251-9
Ali Aydın, Ahmet Karadağ, Şaban Tekin, Hüseyin Akbaş
The Au(CN)2? ion and its metal-ligand compounds have recently gained considerable interest in industrial applications such as optical diagnostics systems as well as pharmacology with antirheumatic and antitumor activity. Here, [Ni2(N-bishydeten)2][Au(μ-CN)2]3[Au(CN)2]·H2O (C1), [Cu2(N-bishydeten)2][Au(μ-CN)2]3[Au(CN)2]·H2O (C2), and [Zn2(μ-N-bishydetenH)(N-bishydeten)(NC)2Au][Au(CN)2] (C3) were synthesized by reaction of the metal salts with N,N-bis(2-hydroxyethyl)ethylenediamine (N-bisyhdeten) and K[Au(CN)2]. The Au(I) compounds were characterized using elemental analysis and FT-IR. ESI-MS and thermal measurement techniques and their pharmacological properties were also tested. The DNA/bovine serum albumin (BSA) interactions of these compounds were demonstrated by spectrophotometric titration, fluorometric ethidium bromide kinetics, and DNA electrophoresis studies, and the stability of these compounds in physiological solution was also determined. The findings indicate that these compounds displayed a DNA/BSA-binding activity similar to that of cisplatin and exhibited a strong aqueous stability. The Au(I) compounds were potent antiproliferative agents with low necrotic activity and exhibited dose-dependent growth inhibition of cancer cells with IC50 value of 0.12–0.73?μM. Accumulation of p53 and decrease in Bcl-2 in cells exposed to Au(I) compounds may be the main causes for apoptotic effects, such as DNA fragmentation and nuclear collapse. Investigations regarding the mode of action of Au(I) compounds on cells revealed that they reduce the cell migration rate and the level of cytoskeletal proteins, namely CK7 and CK20. On the basis of this evidence, we suggest that strong antiproliferative activity, low necrotic effect, and micromolar dose range observed for Au(I) compounds make them suitable candidates for further pharmacological evaluation as chemotherapeutic agents in colon and cervix cancer.
{"title":"Three new dicyanidoaurate(I)-based complexes exhibiting significant antiproliferative property: synthesis and characterization","authors":"Ali Aydın, Ahmet Karadağ, Şaban Tekin, Hüseyin Akbaş","doi":"10.1007/s13404-018-00251-9","DOIUrl":"https://doi.org/10.1007/s13404-018-00251-9","url":null,"abstract":"<p>The Au(CN)<sub>2</sub><sup>?</sup> ion and its metal-ligand compounds have recently gained considerable interest in industrial applications such as optical diagnostics systems as well as pharmacology with antirheumatic and antitumor activity. Here, [Ni<sub>2</sub>(<i>N-bishydeten</i>)<sub>2</sub>][Au(<i>μ</i>-CN)<sub>2</sub>]<sub>3</sub>[Au(CN)<sub>2</sub>]·H<sub>2</sub>O (<b>C1</b>), [Cu<sub>2</sub>(<i>N-bishydeten</i>)<sub>2</sub>][Au(<i>μ</i>-CN)<sub>2</sub>]<sub>3</sub>[Au(CN)<sub>2</sub>]·H<sub>2</sub>O (<b>C2</b>), and [Zn<sub>2</sub>(<i>μ</i>-<i>N-bishydeten</i>H)(<i>N-bishydeten</i>)(NC)<sub>2</sub>Au][Au(CN)<sub>2</sub>] (<b>C3</b>) were synthesized by reaction of the metal salts with <i>N</i>,<i>N</i>-bis(2-hydroxyethyl)ethylenediamine (<i>N-bisyhdeten</i>) and K[Au(CN)<sub>2</sub>]. The Au(I) compounds were characterized using elemental analysis and FT-IR. ESI-MS and thermal measurement techniques and their pharmacological properties were also tested. The DNA/bovine serum albumin (BSA) interactions of these compounds were demonstrated by spectrophotometric titration, fluorometric ethidium bromide kinetics, and DNA electrophoresis studies, and the stability of these compounds in physiological solution was also determined. The findings indicate that these compounds displayed a DNA/BSA-binding activity similar to that of cisplatin and exhibited a strong aqueous stability. The Au(I) compounds were potent antiproliferative agents with low necrotic activity and exhibited dose-dependent growth inhibition of cancer cells with IC50 value of 0.12–0.73?μM. Accumulation of p53 and decrease in Bcl-2 in cells exposed to Au(I) compounds may be the main causes for apoptotic effects, such as DNA fragmentation and nuclear collapse. Investigations regarding the mode of action of Au(I) compounds on cells revealed that they reduce the cell migration rate and the level of cytoskeletal proteins, namely CK7 and CK20. On the basis of this evidence, we suggest that strong antiproliferative activity, low necrotic effect, and micromolar dose range observed for Au(I) compounds make them suitable candidates for further pharmacological evaluation as chemotherapeutic agents in colon and cervix cancer.</p>","PeriodicalId":581,"journal":{"name":"Gold Bulletin","volume":"52 1","pages":"35 - 50"},"PeriodicalIF":2.2,"publicationDate":"2019-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s13404-018-00251-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4119083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.17104/9783406732133-110
B. Grewe
{"title":"Stagnierende Goldpreise, das Ende der südafrikanischen Dominanz und neue Märkte","authors":"B. Grewe","doi":"10.17104/9783406732133-110","DOIUrl":"https://doi.org/10.17104/9783406732133-110","url":null,"abstract":"","PeriodicalId":581,"journal":{"name":"Gold Bulletin","volume":"18 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86189491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.17104/9783406732133-100
B. Grewe
{"title":"Das Ende des amerikanischen Goldstandards","authors":"B. Grewe","doi":"10.17104/9783406732133-100","DOIUrl":"https://doi.org/10.17104/9783406732133-100","url":null,"abstract":"","PeriodicalId":581,"journal":{"name":"Gold Bulletin","volume":"54 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86727880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.17104/9783406732133-47
B. Grewe
{"title":"Die Edelmetallschwemme und ihre Folgen für Europa","authors":"B. Grewe","doi":"10.17104/9783406732133-47","DOIUrl":"https://doi.org/10.17104/9783406732133-47","url":null,"abstract":"","PeriodicalId":581,"journal":{"name":"Gold Bulletin","volume":"115 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78115969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.17104/9783406732133-41
B. Grewe
{"title":"Das Gold der Neuen Welt","authors":"B. Grewe","doi":"10.17104/9783406732133-41","DOIUrl":"https://doi.org/10.17104/9783406732133-41","url":null,"abstract":"","PeriodicalId":581,"journal":{"name":"Gold Bulletin","volume":"11 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86116522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
扫码关注我们
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号