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Roles of epidural block in combination with general anesthesia in stress response and immune function of patients after surgery for cervical cancer 硬膜外阻滞结合全身麻醉对宫颈癌术后患者应激反应和免疫功能的影响。
IF 2.9 4区 生物学 Q3 CELL BIOLOGY Pub Date : 2024-09-23 DOI: 10.1007/s10735-024-10266-6
Bingjie Zhao, Yang Liu, Huan Lu

We aimed to explore the roles of epidural block in combination with general anesthesia in the stress response and immune function of patients after surgery for cervical cancer. A total of 108 patients undergoing radical surgery of cervical cancer were randomly assigned into a general anesthesia combined with epidural block (observation) group and a general anesthesia (control) group. Peripheral blood was collected before anesthesia (t0), during anesthesia maintenance, as well as 10 min, 1 d, 2 d and 7 d after surgery. The levels of cytokines interferon-γ (IFN-γ), interleukin-4 (IL-4) and transforming growth factor-β1 (TGF-β1) were detected by ELISA, and IFN-γ/IL-4 ratio was calculated. Compared with the control group, the observation group had significantly lower levels of GH, PRL and Cor, proportions of Th2 and Treg cells, and levels of IL-4 and TGF-β1 during anesthesia maintenance and at each time point after surgery (P < 0.05), but higher proportion of Th1 cells, Th1/Th2 cell ratio, IFN-γ level and IFN-γ/IL-4 ratio (P < 0.05). General anesthesia in combination with epidural block can work better in mitigating the stress response and protecting the immune function of patients after cervical cancer surgery.

我们旨在探讨硬膜外阻滞结合全身麻醉对宫颈癌术后患者应激反应和免疫功能的影响。我们将 108 名接受宫颈癌根治术的患者随机分为全身麻醉联合硬膜外阻滞组(观察组)和全身麻醉组(对照组)。分别在麻醉前(t0)、麻醉维持期间、术后 10 分钟、1 天、2 天和 7 天采集外周血。用 ELISA 方法检测细胞因子干扰素-γ(IFN-γ)、白细胞介素-4(IL-4)和转化生长因子-β1(TGF-β1)的水平,并计算 IFN-γ/IL-4 的比值。与对照组相比,观察组在麻醉维持期间和术后各时间点的 GH、PRL 和 Cor 水平、Th2 和 Treg 细胞比例、IL-4 和 TGF-β1 水平均显著降低(P<0.05)。
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引用次数: 0
FAM83H regulated by glis3 promotes triple-negative breast cancer tumorigenesis and activates the NF-κB signaling pathway 由 glis3 调控的 FAM83H 可促进三阴性乳腺癌肿瘤发生并激活 NF-κB 信号通路。
IF 2.9 4区 生物学 Q3 CELL BIOLOGY Pub Date : 2024-09-21 DOI: 10.1007/s10735-024-10268-4
Chenhao Li, Xin Wang, Dongliang Shi, Meng Yang, Wenhua Yang, Liang Chen

Triple-negative breast cancer (TNBC) is a highly aggressive and invasive form of breast cancer (BC) with a high mortality rate and a lack of effective targeted drugs. Family with sequence similarity 83 member H (FAM83H) is critically implicated in tumorigenesis. However, the potential role of FAM83H in TNBC remains elusive. Here, we discovered that FAM83H exhibited high expression in tumor tissues of patients with TNBC and was associated with TNM stage. Gain- or loss-of-function experiments were conducted to explore the biological role of FAM83H in TNBC. Subsequently, functional enrichment analysis confirmed that FAM83H overexpression promoted TNBC cell proliferation, invasion, migration and epithelial-mesenchymal transition (EMT), accompanied by upregulation of cyclin E, cyclin D, Vimentin, N-cadherin and Slug. As observed, FAM83H knockdown showed anti-cancer effects, such as fostering apoptosis and inhibiting tumorigenicity and metastasis of TNBC cells. Mechanistically, FAM83H activated the NF-κB signaling pathway. Moreover, a dual-luciferase reporter assay demonstrated that GLIS family zinc finger 3 (GLIS3) bound to the promoter of FAM83H and enhanced its transcription. Notably, overexpression of GLIS3 significantly stimulated TNBC cell proliferation and invasion, and all of this was reversed by rescue experiments involving the knockdown of FAM83H. Overall, FAM83H exacerbates tumor progression, and in-depth understanding of FAM83H as a therapeutic target for TNBC will provide clinical translational potential for intervention therapy.

三阴性乳腺癌(TNBC)是一种侵袭性极强的乳腺癌(BC),死亡率高,且缺乏有效的靶向药物。序列相似性家族 83 成员 H(FAM83H)与肿瘤发生有重要关系。然而,FAM83H在TNBC中的潜在作用仍然难以捉摸。在这里,我们发现 FAM83H 在 TNBC 患者的肿瘤组织中高表达,并且与 TNM 分期相关。为了探索FAM83H在TNBC中的生物学作用,我们进行了功能增益或缺失实验。随后,功能富集分析证实,FAM83H的过表达促进了TNBC细胞的增殖、侵袭、迁移和上皮-间质转化(EMT),并伴随着细胞周期蛋白E、细胞周期蛋白D、Vimentin、N-钙粘蛋白和Slug的上调。据观察,FAM83H敲除具有抗癌作用,如促进凋亡、抑制TNBC细胞的致瘤性和转移。从机制上看,FAM83H激活了NF-κB信号通路。此外,双荧光素酶报告实验表明,GLIS家族锌指3(GLIS3)与FAM83H的启动子结合并增强了其转录。值得注意的是,GLIS3的过表达会显著刺激TNBC细胞的增殖和侵袭,而通过敲除FAM83H的拯救实验,所有这一切都被逆转了。总之,FAM83H会加剧肿瘤的进展,深入了解FAM83H作为TNBC的治疗靶点将为干预治疗提供临床转化潜力。
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引用次数: 0
Correction: Dendrobine alleviates oleic acid-induced lipid accumulation by inhibiting FOS/METTL14 pathway 更正:石斛碱通过抑制 FOS/METTL14 通路缓解油酸诱导的脂质积累。
IF 2.9 4区 生物学 Q3 CELL BIOLOGY Pub Date : 2024-09-19 DOI: 10.1007/s10735-024-10262-w
Junpei Zhang, Hongyun Zhang, Ying Chen, Shiyao Chen, Hailing Liu
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引用次数: 0
Effects of diazinon on the ovarian tissue of rats: a histochemical and ultrastructural study 二嗪农对大鼠卵巢组织的影响:组织化学和超微结构研究
IF 2.9 4区 生物学 Q3 CELL BIOLOGY Pub Date : 2024-09-16 DOI: 10.1007/s10735-024-10261-x
Feras Abou Hasan, Hasan Serdar Mutlu, İlkay Özdemir, Tuğba Kotil

Despite the negative environmental and biologic effects, organophosphates have currently been widely used. We aimed to examine the possible negative effects of diazinon, a type of organophosphate, on rat ovarian tissue. Wistar Albino rats were divided into four groups. No treatment was given to control, olive oil was applied to sham group. Experimental groups were injected intraperitoneally with 30 and 60 mg/kg/day diazinon, respectively. 24 h later, ovarian tissues were extracted, preparated, examined via light and electron microscope. In the experimental groups granulosa and corpus luteum showed degenerative changes. Dilatation of endoplasmic reticulum cisterns and morphological alterations of mitochondria in granulosa cells were detected utrastructurally. Also, accumulation of lipid droplets and autophagic vacuoles was observed in cells of corpus luteum. A statistically significant dose-dependent decrease in superoxide dismutase and catalase reactivity and a statistically significant increase in caspase-3 expression in cells of atretic follicles and corpus luteum were observed. Results show that exposure to a single dose of diazinon may disrupt antioxidant system, trigger atresia in follicles and negatively effect corpus luteum functions. It was concluded that studies applying possible antioxidant treatments should be carried out to reduce and prevent the negative effects of diazinon on the reproductive system.

尽管有机磷对环境和生物有负面影响,但目前仍被广泛使用。我们的目的是研究重氮农(有机磷的一种)对大鼠卵巢组织可能产生的负面影响。Wistar 白化大鼠被分为四组。对照组不做任何处理,假组涂抹橄榄油。实验组分别腹腔注射 30 和 60 毫克/千克/天的二嗪农。24 小时后,提取、制备卵巢组织,并通过光镜和电子显微镜进行检查。实验组的颗粒细胞和黄体出现了退行性变化。从结构上看,颗粒细胞的内质网腔扩张,线粒体形态改变。此外,还在黄体细胞中观察到脂滴和自噬空泡的积累。在静止期卵泡和黄体细胞中,超氧化物歧化酶和过氧化氢酶反应性的下降具有统计学意义,而 Caspase-3 的表达则具有统计学意义。结果表明,接触单剂量的二嗪农可能会破坏抗氧化系统,引发卵泡闭锁,并对黄体功能产生负面影响。研究得出结论,应进行可能的抗氧化治疗研究,以减少和预防二嗪农对生殖系统的负面影响。
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引用次数: 0
Correction: Zinc-alkaline phosphatase at sites of aortic calcification 更正:主动脉钙化部位的锌-碱性磷酸酶。
IF 2.9 4区 生物学 Q3 CELL BIOLOGY Pub Date : 2024-09-14 DOI: 10.1007/s10735-024-10265-7
Santiago Gomez, José Luis Millán
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引用次数: 0
Dehydrozingerone ameliorates arsenic-induced reproductive toxicity in male Wistar rats 脱氢生姜酮可改善砷诱导的雄性 Wistar 大鼠生殖毒性
IF 2.9 4区 生物学 Q3 CELL BIOLOGY Pub Date : 2024-09-13 DOI: 10.1007/s10735-024-10255-9
Anuj Choudhary, Ruchi Pandey, Dipak Rathod, Suhani Sumalatha, Krishna Murti, Velayutham Ravichandiran, Nitesh Kumar

Arsenic (As3+), a significant environmental pollutant that has garnered global attention, is widely recognized for its adverse effects on reproductive health. This study assesses the aphrodisiac activity of Dehydrozingerone (DHZ) against As3+ induced sexual dysfunction in male Wistar rats. Male Wistar rats were divided into control, As3+, and As3++DHZ groups. The As3+ group received 5 mg/kg sodium arsenite (NaAsO2) orally while As3++DHZ group received 50 mg/kg synthesized DHZ along with As3+ for 42 days. Following administration, mount and intromission latency, frequency, and average time were measured to assess aphrodisiac and reproductive toxicity in male Wistar rats which had 1:1 coitus with female rats. On days 14th, 28th, and 42nd, sexual behaviour was measured. Further on 43rd day, animals were sacrificed, blood was collected to measure oxidative parameters and LH hormone, and then testes were collected to profile reproductive damage. As3+ treated rats had lower sperm counts, motility, and abnormalities. These alterations reduced sexual hormones. In addition, As3+ toxicity depleted antioxidant indicators including SOD, GSH and elevated ROS. Compared to the As3+ group, As3++DHZ showed a substantial (p < 0.05) increase in sperm count, motility, and reduced abnormalities. DHZ also reversed the rise in luteinizing hormone caused by As3+ therapy, restored oxidative indicators, and improved seminiferous tubule structural damage. 42 days As3+ exposure slightly increased rats’ sexual desire but not sperm quality. However, As3++DHZ lower libido and sperm quality. Thus, DHZ therapy enhanced rat sexual desire and sperm quality compared to As3+.

砷(As3+)是一种引起全球关注的重要环境污染物,其对生殖健康的不利影响已得到广泛认可。本研究评估了去氢姜酮(DHZ)对 As3+ 诱导的雄性 Wistar 大鼠性功能障碍的壮阳活性。雄性 Wistar 大鼠被分为对照组、As3+ 组和 As3++DHZ 组。As3+ 组口服 5 mg/kg 亚砷酸钠(NaAsO2),As3++DHZ 组口服 50 mg/kg 合成的 DHZ 和 As3+,共 42 天。给药后,测量雄性 Wistar 大鼠与雌性大鼠 1:1 交配时的勃起和插入潜伏期、频率和平均时间,以评估壮阳和生殖毒性。在第 14、28 和 42 天,对性行为进行测量。第 43 天,动物被处死,收集血液以测量氧化参数和 LH 激素,然后收集睾丸以分析生殖损伤。经 As3+ 处理的大鼠精子数量、活力和畸形率都较低。这些变化降低了性激素。此外,As3+毒性还消耗了抗氧化指标,包括SOD、GSH和ROS的升高。与As3+组相比,As3++DHZ组的精子数量、活力和畸形率大幅增加(p < 0.05)。DHZ还能逆转As3+治疗引起的黄体生成素升高,恢复氧化指标,改善曲细精管结构损伤。42天的As3+暴露可轻微提高大鼠的性欲,但不能提高精子质量。然而,As3++DHZ会降低性欲和精子质量。因此,与As3+相比,DHZ疗法能提高大鼠的性欲和精子质量。
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引用次数: 0
A key regulator of tumor-associated neutrophils: the CXCR2 chemokine receptor 肿瘤相关中性粒细胞的关键调节因子:CXCR2 趋化因子受体
IF 2.9 4区 生物学 Q3 CELL BIOLOGY Pub Date : 2024-09-13 DOI: 10.1007/s10735-024-10260-y
Wenyan Kang, Chengkun Wang, Minhui Wang, Meiqi Liu, Wei Hu, Xiaoqiu Liang, Juanli Yang, Yang Zhang

In recent years, with the advance of research, the role of tumor-associated neutrophils (TANs) in tumors has become a research hotspot. As important effector cells in the innate immune system, neutrophils play a key role in the immune and inflammatory responses of the body. As the first line of defense against bacterial and fungal infections, neutrophils have the ability to kill invading pathogens. In the pathological state of malignant tumors, the phenotype of neutrophils is altered and has an important regulatory function in tumor development. The C-X-C motif chemokine receptor 2(CXCR2) is a key molecule that mediates the migration and aggregation signaling pathway of immune cells, especially neutrophils. This review focuses on the regulation of CXCR2 on TANs in the process of tumorigenesis and development, and emphasizes the application significance of CXCR2 inhibitors in blocking the migration of TANs to tumors.

近年来,随着研究的深入,肿瘤相关中性粒细胞(TANs)在肿瘤中的作用成为研究热点。作为先天性免疫系统的重要效应细胞,中性粒细胞在机体的免疫和炎症反应中发挥着关键作用。作为抵御细菌和真菌感染的第一道防线,中性粒细胞具有杀死入侵病原体的能力。在恶性肿瘤的病理状态下,中性粒细胞的表型会发生改变,在肿瘤发生发展过程中具有重要的调控功能。C-X-C motif趋化因子受体2(CXCR2)是介导免疫细胞尤其是中性粒细胞迁移和聚集信号通路的关键分子。本综述重点探讨了CXCR2在肿瘤发生和发展过程中对TANs的调控作用,并强调了CXCR2抑制剂在阻断TANs向肿瘤迁移方面的应用意义。
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引用次数: 0
METTL3 silencing inhibits ferroptosis to suppress ovarian fibrosis in PCOS by upregulating m6A modification of GPX4 通过上调 GPX4 的 m6A 修饰,沉默 METTL3 可抑制多囊卵巢综合症患者的铁蛋白沉积,从而抑制卵巢纤维化
IF 2.9 4区 生物学 Q3 CELL BIOLOGY Pub Date : 2024-09-11 DOI: 10.1007/s10735-024-10257-7
Chuan Shen, Yongmei Jiang, Jia Lin, Qiwei Guo, Dingzhi Fang

Methyltransferase-like 3 (METTL3) is extensively reported to be involved in organ fibrosis. Ovarian fibrosis is a main characteristic of polycystic ovary syndrome (PCOS). However, the reaction mechanism of METTL3 in PCOS is poorly investigated. This paper was intended to reveal the role and the mechanism of METTL3 in PCOS. Animal and cell models of PCOS were induced by dehydroepiandrosterone (DHEA). H&E staining was performed to detect the pathological alterations in ovary tissues. Masson staining, immunofluorescence, along with western blot measured fibrosis both in vitro and in vivo. To evaluate estrous cycle, vaginal smear was performed. Lipid peroxidation and ferroptosis were evaluated by MDA assay kits, GSH assay kits, immunohistochemistry, Prussian blue staining and western blot. qRT-PCR and western blot were adopted to estimate METTL3 and GPX4 expression. The m6A and hormone secretion levels were respectively assessed by m6A RNA Methylation Quantitative Kit and corresponding kits. The interaction between METTL3 and GPX4 was testified by immunoprecipitation. The fibrosis and ferroptosis were aggravated and m6A and METTL3 expression were increased in ovarian tissues of DHEA-induced PCOS mice. METTL3 silencing alleviated pathological changes, affected hormone secretion level, and repressed fibrosis, lipid peroxidation and ferroptosis in the ovarian tissues of PCOS mice. In vitro, DHEA stimulation increased m6A and METTL3 expression and induced ferroptosis and fibrosis. METTL3 knockdown promoted GPX4 expression in DHEA-induced granulosa cells by m6A modification and restrained DHEA-induced fibrosis, lipid peroxidation and ferroptosis in granulosa cells via elevating GPX4. METTL3 silence inhibited ovarian fibrosis in PCOS, which was mediated through suppressing ferroptosis by upregulating GPX4 in m6A-dependent manner.

据广泛报道,甲基转移酶样 3(METTL3)与器官纤维化有关。卵巢纤维化是多囊卵巢综合征(PCOS)的一个主要特征。然而,关于 METTL3 在多囊卵巢综合症中的反应机制研究甚少。本文旨在揭示 METTL3 在多囊卵巢综合征中的作用和机制。通过脱氢表雄酮(DHEA)诱导多囊卵巢综合征的动物和细胞模型。H&E染色检测卵巢组织的病理改变。Masson染色、免疫荧光和Western blot检测了体外和体内的纤维化。为了评估发情周期,进行了阴道涂片检查。通过 MDA 检测试剂盒、GSH 检测试剂盒、免疫组化、普鲁士蓝染色和 Western 印迹来评估脂质过氧化和铁中毒。m6A RNA甲基化定量试剂盒和相应的试剂盒分别评估了m6A和激素分泌水平。免疫沉淀法检测了METTL3和GPX4之间的相互作用。DHEA诱导的多囊卵巢综合征小鼠卵巢组织纤维化和铁沉着加重,m6A和METTL3表达增加。沉默 METTL3 可减轻 PCOS 小鼠卵巢组织的病理变化,影响激素分泌水平,抑制纤维化、脂质过氧化和铁突变。在体外,DHEA 刺激会增加 m6A 和 METTL3 的表达,诱导铁变态反应和纤维化。敲除 METTL3 可通过 m6A 修饰促进 GPX4 在 DHEA 诱导的颗粒细胞中的表达,并通过提高 GPX4 抑制 DHEA 诱导的颗粒细胞纤维化、脂质过氧化和铁败坏。METTL3 沉默可抑制多囊卵巢综合症患者的卵巢纤维化,这种抑制是通过上调 GPX4 以 m6A 依赖性方式抑制铁变态反应来实现的。
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引用次数: 0
Expression of cancer susceptibility candidate 11 in ovarian cancer tissues and its role in doxorubicin resistance 癌症易感性候选基因 11 在卵巢癌组织中的表达及其在多柔比星耐药性中的作用。
IF 2.9 4区 生物学 Q3 CELL BIOLOGY Pub Date : 2024-09-09 DOI: 10.1007/s10735-024-10254-w
Kui Yao, Heng Zheng, Longxia Tong

We aimed to investigate the expression of cancer susceptibility candidate 11 (CASC11) in ovarian cancer (OC) tissues and its role in doxorubicin (Dox) resistance. A total of 98 patients were included as subjects. Reverse transcription-polymerase chain reaction was employed to determine the expressions of CASC11 in OC and para-OC tissues, and in OC cells (A2780, SKOV3, OVCAR3 and A547) and human normal ovarian epithelial cells (IOSE-80) from these patients. OC SKOV3/R cell line with Dox resistance was established and transfected with small interfering (si)-CASC11 to down-regulate CASC11 expression. Based on the constructed nude mouse model of orthotopic transplanted tumor, the growth curves were plotted, and the changes in tumor volume and apoptosis were observed by hematoxylin-eosin staining. OC tissues had a significantly higher mRNA expression of CASC11 than that of para-OC tissues (P < 0.05). A547, OVCAR3, A2780 and SKOV3 cells had significantly higher mRNA expressions of CASC11 than that of IOSE-80 cells (P < 0.05). The transplanted tumor was significantly smaller in volume in the si-CASC11 group than that in the si-normal control (NC) group from the 8th days after transplanted tumor inoculation (P < 0.05). The tumor growth inhibition rate significantly rose in the si-CASC11 group in comparison with that in the si-NC group (P < 0.05). CASC11 has high expression in OC tissues. Knockout of CASC11 weakens the proliferative, invasive and migratory potentials and enhances the apoptotic potential of Dox-resistant OC cells, thereby reversing their Dox resistance.

我们旨在研究癌症易感性候选基因 11(CASC11)在卵巢癌(OC)组织中的表达及其在多柔比星(Dox)耐药性中的作用。研究对象包括98名患者。研究人员采用反转录聚合酶链反应检测了 CASC11 在卵巢癌和副卵巢癌组织中的表达,以及在这些患者的卵巢癌细胞(A2780、SKOV3、OVCAR3 和 A547)和人类正常卵巢上皮细胞(IOSE-80)中的表达。建立了具有Dox耐药性的OC SKOV3/R细胞系,并用小干扰(si)-CASC11转染以下调CASC11的表达。在构建的裸鼠正位移植肿瘤模型基础上,绘制肿瘤生长曲线,并通过苏木精-伊红染色观察肿瘤体积和凋亡的变化。OC组织的CASC11 mRNA表达量明显高于副OC组织(P<0.05)。
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引用次数: 0
Investigating cryopreservation techniques for maintaining morphology and in vitro viability of cartilage and skin from Spix’s yellow-toothed cavies (Galea spixii Wagler, 1831) for conservation through biobanks 研究冷冻保存技术,以保持斯皮克斯黄齿狸猫(Galea spixii Wagler, 1831)软骨和皮肤的形态和体外生存能力,以便通过生物库进行保存。
IF 2.9 4区 生物学 Q3 CELL BIOLOGY Pub Date : 2024-09-09 DOI: 10.1007/s10735-024-10259-5
Samara Lima Olindo, Leonardo Vitorino Costa de Aquino, Yasmin Beatriz França Moura, Yara Letícia Frutuoso e Silva, Ana Lívia Rocha Rodrigues, Vinicius Dantas da Silva, Alexsandra Fernandes Pereira

Conservation of the genetic diversity through skin and cartilage biobanks represents an essential strategy for maintaining biodiversity. Biobanks for the wild species of the order Rodentia have been little studied. Considering that the cryopreservation technique has specific relationships with the tissue and species of interest, we propose investigating different techniques for preserving tissue integrity and cell viability after cartilage and skin culture from Spix’s yellow-toothed cavies. Subsequently, two techniques [solid-surface vitrification (SSV) vs. slow freezing (SF)] were used for cartilage and skin cryopreservation. Tissues not subjected to cryopreservation were used as controls. All tissues were evaluated for morphology and proliferation by histological techniques. Moreover, fragments were cultured, and cells were evaluated for viability, proliferation, metabolism, and apoptosis. Regardless of the cryopreservation technique, no differences were observed for the thickness of the epidermis, dermis, skin, spinous and basal layers, fibroblasts, and proliferative activity regarding the number of nucleolar organizer regions (NOR). SSV ensured better maintenance of epidermal cells, normal chondrocytes, filled gaps, collagen fibers, proliferative activity by NOR area/cell, and reduced perinuclear halos and empty gaps compared to SF. SF ensured the conservation of corneum thickness compared to the control. Although both techniques promoted cell recovery after culture, cells from SF resulted in better subconfluence time and day with cell growth around fragments compared to SSV. In conclusion, both cryopreservation techniques resulted in viable cells after culture. However, SSV promoted better maintenance of tissue morphological integrity, and SF ensured the preservation of all cell quality parameters in Spix’s yellow-toothed cavies.

通过皮肤和软骨生物库保护遗传多样性是维护生物多样性的一项重要战略。对啮齿目野生物种生物库的研究很少。考虑到低温保存技术与所关注的组织和物种有特定的关系,我们建议研究不同的技术,以保存斯皮克斯黄齿狸软骨和皮肤培养后的组织完整性和细胞活力。随后,两种技术[固体表面玻璃化(SSV)与缓慢冷冻(SF)]被用于软骨和皮肤的冷冻保存。未进行冷冻保存的组织被用作对照组。所有组织均通过组织学技术进行形态和增殖评估。此外,还对碎片进行了培养,并对细胞的活力、增殖、新陈代谢和凋亡进行了评估。无论采用哪种冷冻保存技术,表皮、真皮、皮肤、棘层和基底层、成纤维细胞的厚度以及核组织区(NOR)数量方面的增殖活性均无差异。与 SF 相比,SSV 能更好地保持表皮细胞、正常软骨细胞、填充间隙、胶原纤维、NOR 面积/细胞的增殖活性,并减少核周晕和空隙。与对照组相比,SF 确保了角质层厚度的保持。虽然两种技术都能促进细胞培养后的恢复,但与 SSV 相比,SF 细胞的亚融合时间和细胞在碎片周围生长的天数更长。总之,两种冷冻保存技术都能使培养后的细胞存活。不过,SSV能更好地保持组织形态的完整性,而SF则能确保斯皮克斯黄牙豚所有细胞质量参数的保存。
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引用次数: 0
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Journal of Molecular Histology
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