首页 > 最新文献

Journal of the American Society for Mass Spectrometry最新文献

英文 中文
Pneumatically Assisted Microfluidic Probe for Enhanced Mass Spectrometry Imaging Performance
IF 3.1 2区 化学 Q2 BIOCHEMICAL RESEARCH METHODS Pub Date : 2025-03-21 DOI: 10.1021/jasms.5c0001110.1021/jasms.5c00011
Li-Xue Jiang,  and , Julia Laskin*, 

A pneumatically assisted microfluidic probe (MFP) with two microfluidic channels has been developed for nanospray desorption electrospray ionization mass spectrometry imaging (nano-DESI MSI) of biological samples. This design simplifies the experimental setup, making it independent of the vacuum suction at the mass spectrometer inlet. The implementation of pneumatically assisted solvent flow through the probe enables stable, high solvent flow rates required to maintain a consistent liquid bridge during high-throughput MSI experiments. This approach addresses challenges associated with using MFP nano-DESI probes on mass spectrometers that have limited vacuum suction and the operation of MFPs with small microfluidic channels. We demonstrate the robustness of the pneumatically assisted MFP with 30 μm channels, which cannot be used for high-throughput MSI experiments without pneumatic assistance, by successfully imaging five mouse brain tissue sections without interruptions.

{"title":"Pneumatically Assisted Microfluidic Probe for Enhanced Mass Spectrometry Imaging Performance","authors":"Li-Xue Jiang,&nbsp; and ,&nbsp;Julia Laskin*,&nbsp;","doi":"10.1021/jasms.5c0001110.1021/jasms.5c00011","DOIUrl":"https://doi.org/10.1021/jasms.5c00011https://doi.org/10.1021/jasms.5c00011","url":null,"abstract":"<p >A pneumatically assisted microfluidic probe (MFP) with two microfluidic channels has been developed for nanospray desorption electrospray ionization mass spectrometry imaging (nano-DESI MSI) of biological samples. This design simplifies the experimental setup, making it independent of the vacuum suction at the mass spectrometer inlet. The implementation of pneumatically assisted solvent flow through the probe enables stable, high solvent flow rates required to maintain a consistent liquid bridge during high-throughput MSI experiments. This approach addresses challenges associated with using MFP nano-DESI probes on mass spectrometers that have limited vacuum suction and the operation of MFPs with small microfluidic channels. We demonstrate the robustness of the pneumatically assisted MFP with 30 μm channels, which cannot be used for high-throughput MSI experiments without pneumatic assistance, by successfully imaging five mouse brain tissue sections without interruptions.</p>","PeriodicalId":672,"journal":{"name":"Journal of the American Society for Mass Spectrometry","volume":"36 4","pages":"883–887 883–887"},"PeriodicalIF":3.1,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143745881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Investigation of Surface-Induced Dissociation Processes via Molecular Dynamics Simulations of Wall Collisions of Large Droplets Produced by Electrospray Ionization
IF 3.1 2区 化学 Q2 BIOCHEMICAL RESEARCH METHODS Pub Date : 2025-03-21 DOI: 10.1021/jasms.4c0044910.1021/jasms.4c00449
Michelle Rajkovic, Thorsten Benter and Walter Wißdorf*, 

Electrospray ionization is one of the most utilized ionization techniques in atmospheric pressure mass spectrometry. Recent experimentally reported results are in disagreement with fundamentals revolving around ESI droplet sizes and their lifetimes. Specifically, much larger droplet sizes and longer lifetimes have been experimentally observed to exist in typical ESI ion sources. Experiments involving a custom scan mode on a triple quadrupole system have shown that high-mass fragments of large ESI droplets can be observed in mass spectra. Initial hypotheses rationalizing these results were focused on the creation of droplet fragments by collision-induced dissociation (CID). The collision energy accumulated by CID is most likely too small to lead to the observed mass spectra. In response, surface-induced dissociation (SID) was proposed as an additional mechanism to provide large amounts of collision energy to the droplets. The present work thus investigates the possible fragmentation pathways and dynamics of droplet fragments resulting from aspirated ESI droplets upon surface collisions through classical molecular dynamics simulations. Different types of collisions are simulated, where the impact of the simulated droplet fragments is either frontal or angled. The resulting fragmentation dynamics are thoroughly analyzed, showing the possibility for charged fragments to be liberated through SID events. A second, much larger droplet fragment is employed to illustrate the altered collision dynamics found for such larger aggregates, where no charged clusters are released through the surface collision. Since approximated force fields have to be used to model the interactions between the particles observed in the simulation, a sensitivity study is carried out regarding the critical parameters governing such processes. Further modifications of the MD system have to be carried out, including more realistic walls and much larger ESI droplets, to clarify the possibility of charged fragment releases from larger droplet fragments through SID.

{"title":"Investigation of Surface-Induced Dissociation Processes via Molecular Dynamics Simulations of Wall Collisions of Large Droplets Produced by Electrospray Ionization","authors":"Michelle Rajkovic,&nbsp;Thorsten Benter and Walter Wißdorf*,&nbsp;","doi":"10.1021/jasms.4c0044910.1021/jasms.4c00449","DOIUrl":"https://doi.org/10.1021/jasms.4c00449https://doi.org/10.1021/jasms.4c00449","url":null,"abstract":"<p >Electrospray ionization is one of the most utilized ionization techniques in atmospheric pressure mass spectrometry. Recent experimentally reported results are in disagreement with fundamentals revolving around ESI droplet sizes and their lifetimes. Specifically, much larger droplet sizes and longer lifetimes have been experimentally observed to exist in typical ESI ion sources. Experiments involving a custom scan mode on a triple quadrupole system have shown that high-mass fragments of large ESI droplets can be observed in mass spectra. Initial hypotheses rationalizing these results were focused on the creation of droplet fragments by collision-induced dissociation (CID). The collision energy accumulated by CID is most likely too small to lead to the observed mass spectra. In response, surface-induced dissociation (SID) was proposed as an additional mechanism to provide large amounts of collision energy to the droplets. The present work thus investigates the possible fragmentation pathways and dynamics of droplet fragments resulting from aspirated ESI droplets upon surface collisions through classical molecular dynamics simulations. Different types of collisions are simulated, where the impact of the simulated droplet fragments is either frontal or angled. The resulting fragmentation dynamics are thoroughly analyzed, showing the possibility for charged fragments to be liberated through SID events. A second, much larger droplet fragment is employed to illustrate the altered collision dynamics found for such larger aggregates, where no charged clusters are released through the surface collision. Since approximated force fields have to be used to model the interactions between the particles observed in the simulation, a sensitivity study is carried out regarding the critical parameters governing such processes. Further modifications of the MD system have to be carried out, including more realistic walls and much larger ESI droplets, to clarify the possibility of charged fragment releases from larger droplet fragments through SID.</p>","PeriodicalId":672,"journal":{"name":"Journal of the American Society for Mass Spectrometry","volume":"36 4","pages":"760–770 760–770"},"PeriodicalIF":3.1,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143745842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Infrared Matrix-Assisted Laser Desorption Electrospray Ionization (IR-MALDESI) Mass Spectrometry Imaging of Per- and Polyfluoroalkyl Substances (PFAS) in Stabilized Soil Cores
IF 3.1 2区 化学 Q2 BIOCHEMICAL RESEARCH METHODS Pub Date : 2025-03-18 DOI: 10.1021/jasms.4c0042810.1021/jasms.4c00428
Theresa Guillette*, Whitney Stutts, Andrew Baumeister, David Liles, Theresa Olechiw and Johnsie Lang, 

Infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) was coupled with high-resolution accurate-mass–mass spectrometry (HRAM-MS) to image perfluoroalkyl and polyfluoroalkyl substances (PFAS) in stabilized soil cores. Previous field-scale research demonstrated a substantial decrease in the leachability of PFAS following the application of in situ stabilization and solidification (S/S) in an aqueous film-forming foam (AFFF) source zone. While this previous study empirically confirmed the effectiveness of S/S, there was no definitive identification of the operative retention mechanisms. Therefore, the objective of this follow-on study was to (1) develop a high-resolution mass spectrometry-based imaging technique for PFAS on stabilized and background control soil cores and (2) determine if chemical characteristics of the amendments were associated spatially with the PFAS distribution within the soil cores at a micrometer scale. Intact frozen soil cores were imaged in negative ion mode, targeted and suspect screening analyses were conducted, features were identified using suspect lists, and analytes were presented as raw abundances matched against several databases. IR-MALDESI imaging results confirmed the colocation of PFOS and PFHxS with non-PFAS chemical features (e.g., mono- and diglycerides) associated with treatments including amendments, which suggests chemical fixation as a mechanism of stabilization for PFAS in stabilized soil cores.

{"title":"Infrared Matrix-Assisted Laser Desorption Electrospray Ionization (IR-MALDESI) Mass Spectrometry Imaging of Per- and Polyfluoroalkyl Substances (PFAS) in Stabilized Soil Cores","authors":"Theresa Guillette*,&nbsp;Whitney Stutts,&nbsp;Andrew Baumeister,&nbsp;David Liles,&nbsp;Theresa Olechiw and Johnsie Lang,&nbsp;","doi":"10.1021/jasms.4c0042810.1021/jasms.4c00428","DOIUrl":"https://doi.org/10.1021/jasms.4c00428https://doi.org/10.1021/jasms.4c00428","url":null,"abstract":"<p >Infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) was coupled with high-resolution accurate-mass–mass spectrometry (HRAM-MS) to image perfluoroalkyl and polyfluoroalkyl substances (PFAS) in stabilized soil cores. Previous field-scale research demonstrated a substantial decrease in the leachability of PFAS following the application of in situ stabilization and solidification (S/S) in an aqueous film-forming foam (AFFF) source zone. While this previous study empirically confirmed the effectiveness of S/S, there was no definitive identification of the operative retention mechanisms. Therefore, the objective of this follow-on study was to (1) develop a high-resolution mass spectrometry-based imaging technique for PFAS on stabilized and background control soil cores and (2) determine if chemical characteristics of the amendments were associated spatially with the PFAS distribution within the soil cores at a micrometer scale. Intact frozen soil cores were imaged in negative ion mode, targeted and suspect screening analyses were conducted, features were identified using suspect lists, and analytes were presented as raw abundances matched against several databases. IR-MALDESI imaging results confirmed the colocation of PFOS and PFHxS with non-PFAS chemical features (e.g., mono- and diglycerides) associated with treatments including amendments, which suggests chemical fixation as a mechanism of stabilization for PFAS in stabilized soil cores.</p>","PeriodicalId":672,"journal":{"name":"Journal of the American Society for Mass Spectrometry","volume":"36 4","pages":"653–657 653–657"},"PeriodicalIF":3.1,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143745891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Macrocyclic Rearrangement Ion Fragmentation of Glutathione Conjugates of Cyclobutane-Containing Covalent BTK Inhibitors.
IF 3.1 2区 化学 Q2 BIOCHEMICAL RESEARCH METHODS Pub Date : 2025-03-17 DOI: 10.1021/jasms.4c00275
Cathy A Muste, Chungang Gu, H George Vandeveer, Simone Sciabola, Martin K Himmelbauer

Covalent BTK-inhibitor drugs often contain reactive acrylamide warheads designed to irreversibly bind to their protein targets at free thiol cysteines in the kinase active site. This reactivity also makes covalent inhibitors susceptible to conjugation to endogenous tripeptide glutathione (GSH), leading to clearance. During lead optimization efforts for the drug discovery of covalent BTK inhibitor BIIB129, some expected GSH adducts resulted in an unexpected and highly abundant rearrangement fragment ion in LC-MS/MS. By examining more than 30 inhibitors, the rearrangements were found to be dependent on the presence of a cycloalkane linker that connects the warhead to the kinase hinge binder motif of drug molecules. The proposed mechanism includes the formation of a 16-membered macrocyclic intermediate between the γ-glutamic acid residue (Glu) of GSH and a methyl-cyclobutyl cation, resulting in a rearrangement fragment originating from two distant parts of the adduct molecule separated by the warhead conjugated with the cysteine residue in between. Rich sets of chemical analogues available during the lead optimization enabled confirmation of the macrocyclic rearrangement. Proposed macrocyclic rearrangement was verified using GSH derivatives: N-acetylation of the γ-Glu blocked the rearrangement, and esterification of the γ-Glu side chain resulted in an expected shift in the mass of rearranged fragment ion. Proposed rearranged ion structures were supported by MS3 and MS4 fragmentations. Comparisons of the ion fragmentation of GSH conjugates between cis and trans matched pairs suggest a concerted mechanism for the cyclobutane linker and a stepwise mechanism for the methylcyclobutane linker, respectively.

{"title":"Macrocyclic Rearrangement Ion Fragmentation of Glutathione Conjugates of Cyclobutane-Containing Covalent BTK Inhibitors.","authors":"Cathy A Muste, Chungang Gu, H George Vandeveer, Simone Sciabola, Martin K Himmelbauer","doi":"10.1021/jasms.4c00275","DOIUrl":"https://doi.org/10.1021/jasms.4c00275","url":null,"abstract":"<p><p>Covalent BTK-inhibitor drugs often contain reactive acrylamide warheads designed to irreversibly bind to their protein targets at free thiol cysteines in the kinase active site. This reactivity also makes covalent inhibitors susceptible to conjugation to endogenous tripeptide glutathione (GSH), leading to clearance. During lead optimization efforts for the drug discovery of covalent BTK inhibitor BIIB129, some expected GSH adducts resulted in an unexpected and highly abundant rearrangement fragment ion in LC-MS/MS. By examining more than 30 inhibitors, the rearrangements were found to be dependent on the presence of a cycloalkane linker that connects the warhead to the kinase hinge binder motif of drug molecules. The proposed mechanism includes the formation of a 16-membered macrocyclic intermediate between the γ-glutamic acid residue (Glu) of GSH and a methyl-cyclobutyl cation, resulting in a rearrangement fragment originating from two distant parts of the adduct molecule separated by the warhead conjugated with the cysteine residue in between. Rich sets of chemical analogues available during the lead optimization enabled confirmation of the macrocyclic rearrangement. Proposed macrocyclic rearrangement was verified using GSH derivatives: N-acetylation of the γ-Glu blocked the rearrangement, and esterification of the γ-Glu side chain resulted in an expected shift in the mass of rearranged fragment ion. Proposed rearranged ion structures were supported by MS<sup>3</sup> and MS<sup>4</sup> fragmentations. Comparisons of the ion fragmentation of GSH conjugates between <i>cis</i> and <i>trans</i> matched pairs suggest a concerted mechanism for the cyclobutane linker and a stepwise mechanism for the methylcyclobutane linker, respectively.</p>","PeriodicalId":672,"journal":{"name":"Journal of the American Society for Mass Spectrometry","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143646840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Kinetic Method Coupled with Thermal-Assisted Paper Spray Ionization Mass Spectrometry for Direct Determination of Enantiomeric Excess of Multiple d/l-Amino Acids in Functional Foods
IF 3.1 2区 化学 Q2 BIOCHEMICAL RESEARCH METHODS Pub Date : 2025-03-13 DOI: 10.1021/jasms.5c0005010.1021/jasms.5c00050
Wen-Bo Gao, Shu-Ting Xu*, Yong-Jie Yan, Cheng Yang and Xiu-Ping Yan*, 

Amino acids are commonly used as nutritional fortification substances in functional foods, and their chiral configuration is an important determinant of food function. Rapid chiral screening methods are urgently needed in food analysis but are limited by the long-time chiral separation and matrix interference. In this study, we show a kinetic method coupled to thermal-assisted paper spray ionization mass spectrometry for direct determination of enantiomeric excess (ee) of multiple d/l-amino acids in complex food matrixes without sample pretreatment. 3-(2-Naphthyl)-l-alanine was selected as a new chiral reference ligand for the kinetic method to achieve efficient chiral differentiation (discrimination degree is 8.7 for d/l-phenylalanine and 10.2 for d/l-tyrosine). An additional thermal-auxiliary device was developed for paper spray ionization mass spectrometry to facilitate the enantiomeric purity determination. The developed method allowed a rapid simultaneous enantiomeric purity determination of multiple chiral amino acids (d/l-phenylalanine and d/l-tyrosine) within 30 s. Good linearities were achieved for the quantitation of ee (R2 = 0.9996 for phenylalanine and 0.9995 for tyrosine) with unknown amino acid concentrations ranging from 10 μM to 600 μM. The developed method was successfully applied for the enantiomeric purity determination of multiple chiral amino acids in functional capsules and beverages and showed great potential for efficient enantiomer-related food safety screening and nutrition analysis.

{"title":"Kinetic Method Coupled with Thermal-Assisted Paper Spray Ionization Mass Spectrometry for Direct Determination of Enantiomeric Excess of Multiple d/l-Amino Acids in Functional Foods","authors":"Wen-Bo Gao,&nbsp;Shu-Ting Xu*,&nbsp;Yong-Jie Yan,&nbsp;Cheng Yang and Xiu-Ping Yan*,&nbsp;","doi":"10.1021/jasms.5c0005010.1021/jasms.5c00050","DOIUrl":"https://doi.org/10.1021/jasms.5c00050https://doi.org/10.1021/jasms.5c00050","url":null,"abstract":"<p >Amino acids are commonly used as nutritional fortification substances in functional foods, and their chiral configuration is an important determinant of food function. Rapid chiral screening methods are urgently needed in food analysis but are limited by the long-time chiral separation and matrix interference. In this study, we show a kinetic method coupled to thermal-assisted paper spray ionization mass spectrometry for direct determination of enantiomeric excess (<i>ee</i>) of multiple <span>d</span>/<span>l</span>-amino acids in complex food matrixes without sample pretreatment. 3-(2-Naphthyl)-<span>l</span>-alanine was selected as a new chiral reference ligand for the kinetic method to achieve efficient chiral differentiation (discrimination degree is 8.7 for <span>d</span>/<span>l</span>-phenylalanine and 10.2 for <span>d</span>/<span>l</span>-tyrosine). An additional thermal-auxiliary device was developed for paper spray ionization mass spectrometry to facilitate the enantiomeric purity determination. The developed method allowed a rapid simultaneous enantiomeric purity determination of multiple chiral amino acids (<span>d</span>/<span>l</span>-phenylalanine and <span>d</span>/<span>l</span>-tyrosine) within 30 s. Good linearities were achieved for the quantitation of <i>ee</i> (<i>R</i><sup>2</sup> = 0.9996 for phenylalanine and 0.9995 for tyrosine) with unknown amino acid concentrations ranging from 10 μM to 600 μM. The developed method was successfully applied for the enantiomeric purity determination of multiple chiral amino acids in functional capsules and beverages and showed great potential for efficient enantiomer-related food safety screening and nutrition analysis.</p>","PeriodicalId":672,"journal":{"name":"Journal of the American Society for Mass Spectrometry","volume":"36 4","pages":"906–913 906–913"},"PeriodicalIF":3.1,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143746029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Spatial Distribution of Brain PET Tracers by MALDI Imaging
IF 3.1 2区 化学 Q2 BIOCHEMICAL RESEARCH METHODS Pub Date : 2025-03-12 DOI: 10.1021/jasms.4c0030710.1021/jasms.4c00307
Isabeau Vermeulen, Michiel Vandenbosch, Delphine Viot, Joel Mercier, Diego Asensio-Wandosell Cabañas, Pilar Martinez-Martinez, Patrick Barton, Ron M.A. Heeren and Berta Cillero-Pastor*, 

Evaluating tissue distribution of Positron Emission Tomography (PET) tracers during their development conventionally involves autoradiography techniques, where radioactive compounds are used for ex vivo visualization and quantification in tissues during preclinical development stages. Mass Spectrometry Imaging (MSI) offers a potential alternative, providing spatial information without the need for radioactivity with a similar spatial resolution. This study aimed to optimize a MSI sample preparation protocol for assessing PET tracer candidates ex vivo with a focus on two compounds: UCB-J and UCB2400. We tested different matrices and introduced washing steps to improve PET tracer detection. Tissue homogenates were prepared to construct calibration curves for quantification. The incorporation of a washing step into the MSI sample preparation protocol enhanced the signal of both PET tracers. Our findings highlight MSI’s potential as a cost-effective and efficient method for the evaluation of PET tracer distribution. The optimized approach offered here can provide a protocol that enhances the signal and minimizes ion suppression effect, which can be valuable for future evaluation of PET tracers in MSI studies.

{"title":"Spatial Distribution of Brain PET Tracers by MALDI Imaging","authors":"Isabeau Vermeulen,&nbsp;Michiel Vandenbosch,&nbsp;Delphine Viot,&nbsp;Joel Mercier,&nbsp;Diego Asensio-Wandosell Cabañas,&nbsp;Pilar Martinez-Martinez,&nbsp;Patrick Barton,&nbsp;Ron M.A. Heeren and Berta Cillero-Pastor*,&nbsp;","doi":"10.1021/jasms.4c0030710.1021/jasms.4c00307","DOIUrl":"https://doi.org/10.1021/jasms.4c00307https://doi.org/10.1021/jasms.4c00307","url":null,"abstract":"<p >Evaluating tissue distribution of Positron Emission Tomography (PET) tracers during their development conventionally involves autoradiography techniques, where radioactive compounds are used for <i>ex vivo</i> visualization and quantification in tissues during preclinical development stages. Mass Spectrometry Imaging (MSI) offers a potential alternative, providing spatial information without the need for radioactivity with a similar spatial resolution. This study aimed to optimize a MSI sample preparation protocol for assessing PET tracer candidates <i>ex vivo</i> with a focus on two compounds: UCB-J and UCB2400. We tested different matrices and introduced washing steps to improve PET tracer detection. Tissue homogenates were prepared to construct calibration curves for quantification. The incorporation of a washing step into the MSI sample preparation protocol enhanced the signal of both PET tracers. Our findings highlight MSI’s potential as a cost-effective and efficient method for the evaluation of PET tracer distribution. The optimized approach offered here can provide a protocol that enhances the signal and minimizes ion suppression effect, which can be valuable for future evaluation of PET tracers in MSI studies.</p>","PeriodicalId":672,"journal":{"name":"Journal of the American Society for Mass Spectrometry","volume":"36 4","pages":"688–698 688–698"},"PeriodicalIF":3.1,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/jasms.4c00307","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143746059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The Role of Nanobubbles in Protein Unfolding during Electrothermal Supercharging
IF 3.1 2区 化学 Q2 BIOCHEMICAL RESEARCH METHODS Pub Date : 2025-03-11 DOI: 10.1021/jasms.4c0047210.1021/jasms.4c00472
George Joseph, Bincy Binny and Andre R Venter*, 

Nanobubbles (NBs) are tiny gas cavities with diameters around 200 nm that remain stable in solution due to their unique properties, including low buoyancy and negative surface charges. Ammonium bicarbonate (ABC) is an alternative buffer to commonly used ammonium acetate during protein analysis by electrospray ionization (ESI) mass spectrometry. The addition of ABC under high voltage and temperature conditions can lead to protein unfolding, a phenomenon termed electrothermal supercharging (ETS). The role of CO2 bubbles in ETS has been hypothesized and disputed. The solution stability of NBs allows for the direct observation of their effects on protein charge states and unfolding, providing insights into the potential role of CO2 bubbles during ETS. A novel method based on flow regime switching using a Tesla valve is employed to generate stable nanobubbles in solution. NBs were also created by sonication and pressure cycling. Nitrogen and carbon dioxide nanobubbles, when produced by flow regime switching and by pressure cycling, unfold proteins such as cytochrome c and ubiquitin but not to the same extent as with ABC addition to the ESI working solution. Complete unfolding of these proteins by NBs only occurs when the ammonium ion is also present in solution. Myoglobin, known to be less structurally stable, does unfold completely under NB influence. Further, amino acids, previously shown to provide stability to proteins under ETS conditions, also prevent unfolding when NBs are present, providing additional support for the role of gas bubbles during ETS.

{"title":"The Role of Nanobubbles in Protein Unfolding during Electrothermal Supercharging","authors":"George Joseph,&nbsp;Bincy Binny and Andre R Venter*,&nbsp;","doi":"10.1021/jasms.4c0047210.1021/jasms.4c00472","DOIUrl":"https://doi.org/10.1021/jasms.4c00472https://doi.org/10.1021/jasms.4c00472","url":null,"abstract":"<p >Nanobubbles (NBs) are tiny gas cavities with diameters around 200 nm that remain stable in solution due to their unique properties, including low buoyancy and negative surface charges. Ammonium bicarbonate (ABC) is an alternative buffer to commonly used ammonium acetate during protein analysis by electrospray ionization (ESI) mass spectrometry. The addition of ABC under high voltage and temperature conditions can lead to protein unfolding, a phenomenon termed electrothermal supercharging (ETS). The role of CO<sub>2</sub> bubbles in ETS has been hypothesized and disputed. The solution stability of NBs allows for the direct observation of their effects on protein charge states and unfolding, providing insights into the potential role of CO<sub>2</sub> bubbles during ETS. A novel method based on flow regime switching using a Tesla valve is employed to generate stable nanobubbles in solution. NBs were also created by sonication and pressure cycling. Nitrogen and carbon dioxide nanobubbles, when produced by flow regime switching and by pressure cycling, unfold proteins such as cytochrome c and ubiquitin but not to the same extent as with ABC addition to the ESI working solution. Complete unfolding of these proteins by NBs only occurs when the ammonium ion is also present in solution. Myoglobin, known to be less structurally stable, does unfold completely under NB influence. Further, amino acids, previously shown to provide stability to proteins under ETS conditions, also prevent unfolding when NBs are present, providing additional support for the role of gas bubbles during ETS.</p>","PeriodicalId":672,"journal":{"name":"Journal of the American Society for Mass Spectrometry","volume":"36 4","pages":"794–800 794–800"},"PeriodicalIF":3.1,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/jasms.4c00472","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143745888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Ligand Conformational and Metal Coordination Isomers in Complexes of Metal Ions and Cyclic Depsipeptides
IF 3.1 2区 化学 Q2 BIOCHEMICAL RESEARCH METHODS Pub Date : 2025-03-11 DOI: 10.1021/jasms.5c0001010.1021/jasms.5c00010
Emmanuel Nkyaagye, Hernando J. Olivos and Thanh D. Do*, 

A critical challenge in the structural characterization of metal complexes in apolar environments is distinguishing transient structural isomers within an ensemble of lower- and higher-order assemblies. These structural variations arise from subtle changes in ligand architecture and metal coordination chemistry, which are often difficult to deconvolute. Here, we utilize ion activation in both drift-tube and cyclic ion mobility spectrometry–mass spectrometry (IMS-MS) to resolve ligand conformational isomerism and metal coordination isomerism in metal sandwich complexes of cyclic depsipeptide ligands known for selective metal ion transport. Our approach reveals that isomerism driven by ligand structural rearrangements exhibits low energy barriers, allowing their interconversion to be captured on the IMS-MS time scale. In contrast, isomers involving distinct metal coordination states are characterized by higher energy barriers, precluding rapid interconversion. These findings establish a direct correlation between isomer distributions and selective metal binding and transport, providing mechanistic insights into the biological functions of cyclic depsipeptides. This work underscores the utility of IMS-MS for disentangling complex structural dynamics in biologically relevant metal–peptide ligand systems.

{"title":"Ligand Conformational and Metal Coordination Isomers in Complexes of Metal Ions and Cyclic Depsipeptides","authors":"Emmanuel Nkyaagye,&nbsp;Hernando J. Olivos and Thanh D. Do*,&nbsp;","doi":"10.1021/jasms.5c0001010.1021/jasms.5c00010","DOIUrl":"https://doi.org/10.1021/jasms.5c00010https://doi.org/10.1021/jasms.5c00010","url":null,"abstract":"<p >A critical challenge in the structural characterization of metal complexes in apolar environments is distinguishing transient structural isomers within an ensemble of lower- and higher-order assemblies. These structural variations arise from subtle changes in ligand architecture and metal coordination chemistry, which are often difficult to deconvolute. Here, we utilize ion activation in both drift-tube and cyclic ion mobility spectrometry–mass spectrometry (IMS-MS) to resolve ligand conformational isomerism and metal coordination isomerism in metal sandwich complexes of cyclic depsipeptide ligands known for selective metal ion transport. Our approach reveals that isomerism driven by ligand structural rearrangements exhibits low energy barriers, allowing their interconversion to be captured on the IMS-MS time scale. In contrast, isomers involving distinct metal coordination states are characterized by higher energy barriers, precluding rapid interconversion. These findings establish a direct correlation between isomer distributions and selective metal binding and transport, providing mechanistic insights into the biological functions of cyclic depsipeptides. This work underscores the utility of IMS-MS for disentangling complex structural dynamics in biologically relevant metal–peptide ligand systems.</p>","PeriodicalId":672,"journal":{"name":"Journal of the American Society for Mass Spectrometry","volume":"36 4","pages":"873–882 873–882"},"PeriodicalIF":3.1,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143746052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Characterizing Monoclonal Antibody Aggregation Using Charge Detection Mass Spectrometry and Industry Standard Methods.
IF 3.1 2区 化学 Q2 BIOCHEMICAL RESEARCH METHODS Pub Date : 2025-03-11 DOI: 10.1021/jasms.4c00503
Jacob S Jordan, Conner C Harper, Fan Zhang, Esther Kofman, Miranda Sam, Jan Paulo Zaragoza, Bhagyashree Bhagwat, Laurence Fayadat-Dilman, Evan R Williams

Protein aggregation is a factor in a multitude of neurodegenerative diseases and aggregates of protein-based biotherapeutics can cause toxicity in vivo and adverse patient outcomes. Monoclonal antibody (M)-fluorophore (F) complexes with four different antibody sequences and masses of ∼680 kDa were analyzed using size-exclusion chromatography (SEC) and mass spectrometry using both quadrupole-time-of-flight (QTOF) and charge detection mass spectrometry (CDMS). Higher-order aggregates were not resolved using SEC, but species as large as the MF2 complex and M5F3 were resolved using QTOF and CDMS, respectively. Results from three freeze-thaw cycles and long-term heat stress indicate that both aggregation and degradation occurs. Two of the antibodies form a critical M2F complex that is sensitive to thermal stress, whereas the other two antibodies undergo degradation and formation of the assembled MF2 complex in response to freeze-thaw and thermal stressors, respectively. These data show that small differences in mAb sequence can result in significant changes to the aggregation and degradation pathways and highlight the promise of combined mass spectrometry approaches for characterizing how various stress factors affect the stability and aggregation propensity of mAbs.

{"title":"Characterizing Monoclonal Antibody Aggregation Using Charge Detection Mass Spectrometry and Industry Standard Methods.","authors":"Jacob S Jordan, Conner C Harper, Fan Zhang, Esther Kofman, Miranda Sam, Jan Paulo Zaragoza, Bhagyashree Bhagwat, Laurence Fayadat-Dilman, Evan R Williams","doi":"10.1021/jasms.4c00503","DOIUrl":"https://doi.org/10.1021/jasms.4c00503","url":null,"abstract":"<p><p>Protein aggregation is a factor in a multitude of neurodegenerative diseases and aggregates of protein-based biotherapeutics can cause toxicity <i>in vivo</i> and adverse patient outcomes. Monoclonal antibody (M)-fluorophore (F) complexes with four different antibody sequences and masses of ∼680 kDa were analyzed using size-exclusion chromatography (SEC) and mass spectrometry using both quadrupole-time-of-flight (QTOF) and charge detection mass spectrometry (CDMS). Higher-order aggregates were not resolved using SEC, but species as large as the MF<sub>2</sub> complex and M<sub>5</sub>F<sub>3</sub> were resolved using QTOF and CDMS, respectively. Results from three freeze-thaw cycles and long-term heat stress indicate that both aggregation and degradation occurs. Two of the antibodies form a critical M<sub>2</sub>F complex that is sensitive to thermal stress, whereas the other two antibodies undergo degradation and formation of the assembled MF<sub>2</sub> complex in response to freeze-thaw and thermal stressors, respectively. These data show that small differences in mAb sequence can result in significant changes to the aggregation and degradation pathways and highlight the promise of combined mass spectrometry approaches for characterizing how various stress factors affect the stability and aggregation propensity of mAbs.</p>","PeriodicalId":672,"journal":{"name":"Journal of the American Society for Mass Spectrometry","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143603335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
TargetSeeker-MS: A Bayesian Inference Approach for Drug–Target Discovery Using Protein Fractionation Coupled to Mass Spectrometry
IF 3.1 2区 化学 Q2 BIOCHEMICAL RESEARCH METHODS Pub Date : 2025-03-11 DOI: 10.1021/jasms.4c0026910.1021/jasms.4c00269
Mathieu Lavallée-Adam, Alexander Pelletier, Jolene K. Diedrich, Antonio F. M. Pinto, Salvador Martínez-Bartolomé, Michael Petrascheck, James J. Moresco and John R. Yates III*, 

To understand the mechanism of action of a drug and assess its clinical usefulness and viability, it is imperative that its affinity for its putative targets is determined. When coupled to mass spectrometry (MS), energetics-based protein separation (EBPS) techniques, such as a thermal shift assay, have shown great potential to identify the targets of a drug on a proteome scale. Nevertheless, the computational analyses assessing the confidence of drug–target predictions made by these methods have remained tightly tied to the protocol under which the data were produced. To identify drug targets in data sets produced using different EBPS-MS techniques, we have developed a novel flexible Bayesian inference approach named TargetSeeker-MS. We showed that TargetSeeker-MS identifies known and novel drug targets in Caenorhabditis elegans and HEK 293 samples treated with the fungicide benomyl. We also demonstrated that TargetSeeker-MS’ drug–target identifications are reproducible in C. elegans samples that were processed using two different EBPS techniques (thermal shift assay and a differential precipitation of proteins, named DiffPOP). In addition, we validated a novel benomyl target by measuring its altered enzymatic activity upon drug treatment in vitro. TargetSeeker-MS, which is available as a web server (https://targetseeker.scripps.edu/), allows for the rapid, versatile, and confident identification of targets of a drug on a proteome scale, thereby providing a better understanding of its mechanisms and facilitating the evaluation of its clinical viability.

{"title":"TargetSeeker-MS: A Bayesian Inference Approach for Drug–Target Discovery Using Protein Fractionation Coupled to Mass Spectrometry","authors":"Mathieu Lavallée-Adam,&nbsp;Alexander Pelletier,&nbsp;Jolene K. Diedrich,&nbsp;Antonio F. M. Pinto,&nbsp;Salvador Martínez-Bartolomé,&nbsp;Michael Petrascheck,&nbsp;James J. Moresco and John R. Yates III*,&nbsp;","doi":"10.1021/jasms.4c0026910.1021/jasms.4c00269","DOIUrl":"https://doi.org/10.1021/jasms.4c00269https://doi.org/10.1021/jasms.4c00269","url":null,"abstract":"<p >To understand the mechanism of action of a drug and assess its clinical usefulness and viability, it is imperative that its affinity for its putative targets is determined. When coupled to mass spectrometry (MS), energetics-based protein separation (EBPS) techniques, such as a thermal shift assay, have shown great potential to identify the targets of a drug on a proteome scale. Nevertheless, the computational analyses assessing the confidence of drug–target predictions made by these methods have remained tightly tied to the protocol under which the data were produced. To identify drug targets in data sets produced using different EBPS-MS techniques, we have developed a novel flexible Bayesian inference approach named TargetSeeker-MS. We showed that TargetSeeker-MS identifies known and novel drug targets in <i>Caenorhabditis elegans</i> and HEK 293 samples treated with the fungicide benomyl. We also demonstrated that TargetSeeker-MS’ drug–target identifications are reproducible in <i>C. elegans</i> samples that were processed using two different EBPS techniques (thermal shift assay and a differential precipitation of proteins, named DiffPOP). In addition, we validated a novel benomyl target by measuring its altered enzymatic activity upon drug treatment in vitro. TargetSeeker-MS, which is available as a web server (https://targetseeker.scripps.edu/), allows for the rapid, versatile, and confident identification of targets of a drug on a proteome scale, thereby providing a better understanding of its mechanisms and facilitating the evaluation of its clinical viability.</p>","PeriodicalId":672,"journal":{"name":"Journal of the American Society for Mass Spectrometry","volume":"36 4","pages":"664–679 664–679"},"PeriodicalIF":3.1,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143746053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
Journal of the American Society for Mass Spectrometry
全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1