Pub Date : 2023-06-27DOI: 10.14720/aas.2023.119.2.2738
V. Sota, Donald Shuka, S. Bekheet, E. Kongjika
Ironwort / Mountain Tea (Sideritis raeseri Boiss & Heldr.) is an endangered (EN) plant species in Albania. This study aimed to develop a rapid clonal propagation protocol using in vitro methodologies. The ironwort seeds were pre-treated with three concentrations of GA3 (250, 500, and 1000 mg l-1). During the inoculation stage, two types of culture media, Murashige & Skoog (MS) and Woody Plant Medium (WPM), were tested, and the effects of both GA3 concentration and culture media used were evaluated. For the subculture stage, three cytokinins (6-benzylaminopurine / BAP, kinetin, zeatin) at four concentrations (0.5; 1.0; 1.5; 2.0 mg l-1), were compared for the RGR index, while for the rooting stage, two different auxins (1-naphthaleneacetic acid / NAA and indole-3-butyric acid / IBA) at four concentrations (0.5; 1.0; 1.5; 2.0 mg l-1) were tested. GA3 at 500 mg l-1 and MS medium resulted as more effective. The highest value of the RGR index during the subculture stage was obtained in the MS nutrient medium supplemented with BAP at 1.5 mg l-1. For rhizogenesis response, IBA was more effective for roots and length number. Based on these results, in vitro methodologies can be a promising tool for the mass production of this endangered plant species and with possible applications for enhancing the production of valuable nutraceuticals.
{"title":"Establishment of an in vitro method for micropropagation of ironwort, (Sideritis raeseri Boiss. & Heldr.)","authors":"V. Sota, Donald Shuka, S. Bekheet, E. Kongjika","doi":"10.14720/aas.2023.119.2.2738","DOIUrl":"https://doi.org/10.14720/aas.2023.119.2.2738","url":null,"abstract":"Ironwort / Mountain Tea (Sideritis raeseri Boiss & Heldr.) is an endangered (EN) plant species in Albania. This study aimed to develop a rapid clonal propagation protocol using in vitro methodologies. The ironwort seeds were pre-treated with three concentrations of GA3 (250, 500, and 1000 mg l-1). During the inoculation stage, two types of culture media, Murashige & Skoog (MS) and Woody Plant Medium (WPM), were tested, and the effects of both GA3 concentration and culture media used were evaluated. For the subculture stage, three cytokinins (6-benzylaminopurine / BAP, kinetin, zeatin) at four concentrations (0.5; 1.0; 1.5; 2.0 mg l-1), were compared for the RGR index, while for the rooting stage, two different auxins (1-naphthaleneacetic acid / NAA and indole-3-butyric acid / IBA) at four concentrations (0.5; 1.0; 1.5; 2.0 mg l-1) were tested. GA3 at 500 mg l-1 and MS medium resulted as more effective. The highest value of the RGR index during the subculture stage was obtained in the MS nutrient medium supplemented with BAP at 1.5 mg l-1. For rhizogenesis response, IBA was more effective for roots and length number. Based on these results, in vitro methodologies can be a promising tool for the mass production of this endangered plant species and with possible applications for enhancing the production of valuable nutraceuticals.","PeriodicalId":6884,"journal":{"name":"Acta agriculturae Slovenica","volume":"56 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89145516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-27DOI: 10.14720/aas.2023.119.2.2657
Nassima Senani, Samia Bedouhène, K. Houali
The insecticides use is important for crop improvement and protection, but in excessive amounts, they would induce a dysfunction of metabolic enzymatic systems in plant tissues, leading to undesirable qualitative changes. In this context, we are interested in peroxidase (POD), an important enzyme in plant physiology but whose activity seems to be conditioned by the presence of insecticides in the soil. This work aims to study the impact of locally used insecticides (chlorpyrifos and dimethoate) on the activity of POD in parsley, onion, celery and garlic grown in soils treated or not. POD extraction was performed using Tris-HCl buffer (pH 7.3); its activity was measured using the substrate o-dianisidine in the presence of H2O2. Our result showed that POD activity for insecticide treated parsley, celery and onions increased by 30 % 127 % and 341 % respectively, however did not change significantly for garlic. Thus, the action of these chemicals is not trivial because they may alter non-target pathways, especially when doses are not adjusted accordingly. We found that insecticide stress would increase POD activity in all vegetables except garlic, which showed tolerance to insecticides. Our findings suggest that organic farming conditions could minimize peroxidase activity in parsley, celery and onion. We add that overproduction of POD negatively affects the quality and reduces the shelf life of vegetables, thus would be a very interesting biomarker of insecticide stress.
{"title":"Peroxidase activity as a biochemical marker of insecticide use in vegetables","authors":"Nassima Senani, Samia Bedouhène, K. Houali","doi":"10.14720/aas.2023.119.2.2657","DOIUrl":"https://doi.org/10.14720/aas.2023.119.2.2657","url":null,"abstract":"The insecticides use is important for crop improvement and protection, but in excessive amounts, they would induce a dysfunction of metabolic enzymatic systems in plant tissues, leading to undesirable qualitative changes. In this context, we are interested in peroxidase (POD), an important enzyme in plant physiology but whose activity seems to be conditioned by the presence of insecticides in the soil. This work aims to study the impact of locally used insecticides (chlorpyrifos and dimethoate) on the activity of POD in parsley, onion, celery and garlic grown in soils treated or not. POD extraction was performed using Tris-HCl buffer (pH 7.3); its activity was measured using the substrate o-dianisidine in the presence of H2O2. Our result showed that POD activity for insecticide treated parsley, celery and onions increased by 30 % 127 % and 341 % respectively, however did not change significantly for garlic. Thus, the action of these chemicals is not trivial because they may alter non-target pathways, especially when doses are not adjusted accordingly. We found that insecticide stress would increase POD activity in all vegetables except garlic, which showed tolerance to insecticides. Our findings suggest that organic farming conditions could minimize peroxidase activity in parsley, celery and onion. We add that overproduction of POD negatively affects the quality and reduces the shelf life of vegetables, thus would be a very interesting biomarker of insecticide stress.","PeriodicalId":6884,"journal":{"name":"Acta agriculturae Slovenica","volume":"49 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86179024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-27DOI: 10.14720/aas.2023.119.2.2949
H. Zaki, Mona Mohamed Mansour, Samah O. Osman, Nagwa R. A. Hussein
Mango (Mangifera indica L.) is one of the tastiest fruits in the world, with numerous advantages beyond their economic value. Eleven genotypes of mango various cultivars were examined for variability, heritability, and genetic advance, as well as multivariate analysis based on cluster and principal component analysis (PCA) for yield and some of its contributing traits during the two growing seasons, 2021 and 2022. All studied traits showed significant differences, and the phenotypic coefficients of variation (PCV) were found to be higher than genotypic coefficients of variation (GCV), supporting the idea that morphological (genetic) traits are more prevalent than environmental influence. All traits had substantial heritability ranging from 75.63 to 99.93 %, and the highest significant genetic advance (119.09 %) was for the number of fruits per tree than other traits. Four clusters were formed, i.e., clusters I and IV had four genotypes, cluster II had two, and cluster III had one genotype. The highest cluster mean values for fruit diameter, fruit mass, yield per tree, and the number of fruits per tree were found in Cluster II, followed by cluster I. Greater genetic divergence was found between ‘Zebda’ or ‘S9’ or ‘S10’ with most other genotypes, indicating that these genotypes may be used to study the characters’ broad range of variability and to yield high-quality recombinant lines. In light of the fact that mango is a very heterozygous crop, our current genetic results can be used for the selection of the appropriate parents in hybridization programs and in vegetative propagation to yield selective traits.
{"title":"Variability of genetic - morphological traits of eleven seed strains of Mangifera indica L. growing in Upper Egypt","authors":"H. Zaki, Mona Mohamed Mansour, Samah O. Osman, Nagwa R. A. Hussein","doi":"10.14720/aas.2023.119.2.2949","DOIUrl":"https://doi.org/10.14720/aas.2023.119.2.2949","url":null,"abstract":"Mango (Mangifera indica L.) is one of the tastiest fruits in the world, with numerous advantages beyond their economic value. Eleven genotypes of mango various cultivars were examined for variability, heritability, and genetic advance, as well as multivariate analysis based on cluster and principal component analysis (PCA) for yield and some of its contributing traits during the two growing seasons, 2021 and 2022. All studied traits showed significant differences, and the phenotypic coefficients of variation (PCV) were found to be higher than genotypic coefficients of variation (GCV), supporting the idea that morphological (genetic) traits are more prevalent than environmental influence. All traits had substantial heritability ranging from 75.63 to 99.93 %, and the highest significant genetic advance (119.09 %) was for the number of fruits per tree than other traits. Four clusters were formed, i.e., clusters I and IV had four genotypes, cluster II had two, and cluster III had one genotype. The highest cluster mean values for fruit diameter, fruit mass, yield per tree, and the number of fruits per tree were found in Cluster II, followed by cluster I. Greater genetic divergence was found between ‘Zebda’ or ‘S9’ or ‘S10’ with most other genotypes, indicating that these genotypes may be used to study the characters’ broad range of variability and to yield high-quality recombinant lines. In light of the fact that mango is a very heterozygous crop, our current genetic results can be used for the selection of the appropriate parents in hybridization programs and in vegetative propagation to yield selective traits.","PeriodicalId":6884,"journal":{"name":"Acta agriculturae Slovenica","volume":"35 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81081046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-27DOI: 10.14720/aas.2023.119.2.2997
Shaghayegh T. Beheshti, M. J. Nazarideljou, M. Salehi
The purpose of this experiment is to compare the growth and water consumption efficiency of five garden plants (marigold (Tagetes erecta ‘Red Brocade’), moss-rose (Portulaca grandiflora ‘Sun Rose’), dahlia (Dahlia sp. ‘Double Opra’), gazania (Gazania splendens ‘New Day’), and Indian blanket (Gaillardia pulchella. ‘Sun Dance’)) during the warmer seasons of the year under various levels of drought stress based on field capacity (FC; 25, 50, 75, and 100 %). The interaction effect of plant × drought stress (FC) on the fresh and dry mass of aerial and underground organs was significant. Decreased water availability resulted in a drop in growth parameters (leaf fresh and dry mass and leaf area). In compared to the growth of aerial organs, root biomass increased in response to drought stress. Marigold, Indian blanket, and dahlia plants had the highest root-to-shoot ratio in extreme stress, i.e., FC 25 %. The plant × drought stress interaction significantly influenced flower number, whereas flower diameter was influenced by the main effect of plant and drought stress (not their interaction). The FC 100 % and FC 25 % treatments had the highest and the lowest accumulations of proline and soluble sugars, respectively. Moss-rose, gazania, and marigold ornamental plants had the highest water use efficiency at 75 %, followed by Dahlia at 50 % and moss-rose at 25 %.
本试验的目的是比较五种园林植物(万寿菊(Tagetes erecta ' Red Brocade ')、月季(Portulaca grandflora ' Sun Rose ')、大丽花(dahlia sp. ' Double Opra ')、栀子花(gazania splendens ' New Day ')和印度毯(Gaillardia pulchella)的生长和耗水效率。“太阳之舞”))，在一年中较温暖的季节，根据田间能力，在不同程度的干旱压力下(FC;25、50、75和100%)。植株×干旱胁迫(FC)对地上和地下器官鲜干质量的互作效应显著。水分有效性降低导致生长参数(叶片鲜干质量和叶面积)下降。与地上器官的生长相比，根系生物量在干旱胁迫下增加。万寿菊、印度毯和大丽花在极端胁迫下的根冠比最高，为25%。植株与干旱胁迫的交互作用显著影响花的数量，而花的直径受植株和干旱胁迫的主效应影响，而不是它们的交互作用。氟化碳100%和氟化碳25%处理的脯氨酸和可溶性糖积累量最高和最低。观赏植物月季、栀子花和万寿菊的水分利用效率最高，为75%，大丽花次之，为50%，月季为25%。
{"title":"Water use efficiency, morpho-physiological and biochemical reactions of some bedding plants to drought stress","authors":"Shaghayegh T. Beheshti, M. J. Nazarideljou, M. Salehi","doi":"10.14720/aas.2023.119.2.2997","DOIUrl":"https://doi.org/10.14720/aas.2023.119.2.2997","url":null,"abstract":"The purpose of this experiment is to compare the growth and water consumption efficiency of five garden plants (marigold (Tagetes erecta ‘Red Brocade’), moss-rose (Portulaca grandiflora ‘Sun Rose’), dahlia (Dahlia sp. ‘Double Opra’), gazania (Gazania splendens ‘New Day’), and Indian blanket (Gaillardia pulchella. ‘Sun Dance’)) during the warmer seasons of the year under various levels of drought stress based on field capacity (FC; 25, 50, 75, and 100 %). The interaction effect of plant × drought stress (FC) on the fresh and dry mass of aerial and underground organs was significant. Decreased water availability resulted in a drop in growth parameters (leaf fresh and dry mass and leaf area). In compared to the growth of aerial organs, root biomass increased in response to drought stress. Marigold, Indian blanket, and dahlia plants had the highest root-to-shoot ratio in extreme stress, i.e., FC 25 %. The plant × drought stress interaction significantly influenced flower number, whereas flower diameter was influenced by the main effect of plant and drought stress (not their interaction). The FC 100 % and FC 25 % treatments had the highest and the lowest accumulations of proline and soluble sugars, respectively. Moss-rose, gazania, and marigold ornamental plants had the highest water use efficiency at 75 %, followed by Dahlia at 50 % and moss-rose at 25 %.","PeriodicalId":6884,"journal":{"name":"Acta agriculturae Slovenica","volume":"99 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77353963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-27DOI: 10.14720/aas.2023.119.2.13280
Mirsade Osmani, M. Tuna, I. Elezaj
The Balkan Peninsula is considered an important centre of native tulip species. Tulipa kosovarica and Tulipa luanica are new species recently discovered in Kosovo, and Tulipa albanica in Albania. The current study aims at the investigating the nuclear DNA content and chromosome number of these three tulipa species in order to provide for the first time data on their genome size and differences among these three Tulipa species. Analysis of nuclear DNA content was performed by flow cytometer (Partec CyFlow Space) in mature fresh leaves for each Tulipa species. Samples for chromosome analysis were taken from the root tip meristem of the bulbs. Results showed significantly higher amounts of nuclear DNA (2C) in T. luanica compared to T. kosovarica and T. albanica. The chromosome number for these three species was 2n = 2x = 24, while the chromosome sizes of T. luanica resulted larger, compared to that of T. kosovarica and T. albanica. A correlation between the nuclear DNA content and chromosome size was found among these tulipa species. Moreover, nuclear DNA content and chromosome sizes of T. luanica, T. kosovarica and T. albanica showed clear differences among these species.
{"title":"Characterization of nuclear DNA content and chromosome numbers of Tulipa luanica Millaku, T. kosovarica Kit Tan, Shuka & Krasniqi and T. albanica Kit Tan & Shuka","authors":"Mirsade Osmani, M. Tuna, I. Elezaj","doi":"10.14720/aas.2023.119.2.13280","DOIUrl":"https://doi.org/10.14720/aas.2023.119.2.13280","url":null,"abstract":"The Balkan Peninsula is considered an important centre of native tulip species. Tulipa kosovarica and Tulipa luanica are new species recently discovered in Kosovo, and Tulipa albanica in Albania. The current study aims at the investigating the nuclear DNA content and chromosome number of these three tulipa species in order to provide for the first time data on their genome size and differences among these three Tulipa species. Analysis of nuclear DNA content was performed by flow cytometer (Partec CyFlow Space) in mature fresh leaves for each Tulipa species. Samples for chromosome analysis were taken from the root tip meristem of the bulbs. Results showed significantly higher amounts of nuclear DNA (2C) in T. luanica compared to T. kosovarica and T. albanica. The chromosome number for these three species was 2n = 2x = 24, while the chromosome sizes of T. luanica resulted larger, compared to that of T. kosovarica and T. albanica. A correlation between the nuclear DNA content and chromosome size was found among these tulipa species. Moreover, nuclear DNA content and chromosome sizes of T. luanica, T. kosovarica and T. albanica showed clear differences among these species.","PeriodicalId":6884,"journal":{"name":"Acta agriculturae Slovenica","volume":"89 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83452717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-27DOI: 10.14720/aas.2023.119.2.2492
Yu. V. Gavrilyuk, I. Aksyonov, Nataliya Matsay, A. Beseda, I. Aksyonova
The article presents the results of many years of research for the period 2003-2020, which were aimed at studying the dissemination and expansion of plant groups of quarantine species of Ambrosia weeds in the eastern steppe of Ukraine. It has been established that the intensive dissemination of the species of the quarantine ragweed weed occurs both naturally and anthropically. Monitoring of the dissemination and growth of ragweed species is noted in all types of phytocenoses of the steppe. The increase in the areas of dissemination of aggressive species of ragweed weeds poses a widespread threat to all plant populations. Studies show a tendency to increase the number of ragweed plants in such plant groups as agrophytocenoses, phytocenoses of planted forests, phytocenoses of urban areas, meadows and pastures. For the period 2011-2002 the number of ragweed plants increased in meadows by 7.5 times, in pastures by 14.8 times, in agrophytocenoses by 2.95 times, in phytocenoses of urban areas by 1.68 times, in phytocenoses of planted forests by 1.28 times. Due to the lack of control over the dissemination and appropriate eradication measures, the largest increase in the number of ragweed plants over the past 10 years has been observed in meadows and pastures. It is recommended in phytocenoses of planted forests and urban areas in meadows and pastures, total mowing of ragweed plants before their flowering 5-7 times during the growing season in order to prevent replenishment of the seed stock of this weed in the soil during the growing season.
{"title":"Dissemination of the quarantine weeds of the genus Ambrosia in the steppe zone of Ukraine","authors":"Yu. V. Gavrilyuk, I. Aksyonov, Nataliya Matsay, A. Beseda, I. Aksyonova","doi":"10.14720/aas.2023.119.2.2492","DOIUrl":"https://doi.org/10.14720/aas.2023.119.2.2492","url":null,"abstract":"The article presents the results of many years of research for the period 2003-2020, which were aimed at studying the dissemination and expansion of plant groups of quarantine species of Ambrosia weeds in the eastern steppe of Ukraine. It has been established that the intensive dissemination of the species of the quarantine ragweed weed occurs both naturally and anthropically. Monitoring of the dissemination and growth of ragweed species is noted in all types of phytocenoses of the steppe. The increase in the areas of dissemination of aggressive species of ragweed weeds poses a widespread threat to all plant populations. Studies show a tendency to increase the number of ragweed plants in such plant groups as agrophytocenoses, phytocenoses of planted forests, phytocenoses of urban areas, meadows and pastures. For the period 2011-2002 the number of ragweed plants increased in meadows by 7.5 times, in pastures by 14.8 times, in agrophytocenoses by 2.95 times, in phytocenoses of urban areas by 1.68 times, in phytocenoses of planted forests by 1.28 times. Due to the lack of control over the dissemination and appropriate eradication measures, the largest increase in the number of ragweed plants over the past 10 years has been observed in meadows and pastures. It is recommended in phytocenoses of planted forests and urban areas in meadows and pastures, total mowing of ragweed plants before their flowering 5-7 times during the growing season in order to prevent replenishment of the seed stock of this weed in the soil during the growing season.","PeriodicalId":6884,"journal":{"name":"Acta agriculturae Slovenica","volume":"6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81495844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-28DOI: 10.14720/aas.2023.119.1.2970
Mehdi Jamshideyni, M. Behdani, S. Parsa, S. Khoramdel
The current study was aim to evaluate the interaction effects of gamma aminobutyric acid (GABA) and vermicompost on yield and yield components of quinoa under different levels of drought stress. For this, two experiments were similarly designed as the factorial-split with four replicates for evaluating this hypothesis. Irrigation regimes (50, 75, and 100 % of plant water requirement (PWR)) as the main plot and vermicompost V (0, 5 t ha-1) × gamma aminobutyric acid GABA levels (0, 5, 10 mg l-1) as the subplot were designed. Severe drought stress had a significant effect on plant height. Plant height reduced 31.8 % after using 50 % of PWR compared to the control conditions. Although drought stress negatively affected the 1000 seed mass and seed yield, GABA foliar application alleviated these effects. After using 50 % of PWR, 10 mg l-1 of GABA increased the seed yield and harvest index up to 21.22 and 15.5 %, respectively, compared to the non-foliar application. The reduction in PWR from 100 to 50 % led to increasing in P and K concentrations, as well as sugar and proline contents. In the same conditions, the use of GABA or V had a significant effect on improving these traits. A similar trend was also recorded in relation to water use efficiency. Therefore, using 10 mg l-1 of GABA and 5 t ha-1 of V can be effective in alleviating water stress.
本研究旨在评价不同干旱胁迫水平下γ氨基丁酸(GABA)和蚯蚓堆肥对藜麦产量及产量成分的互作效应。为此，两个实验被类似地设计为四次重复的因子分裂来评估这一假设。设计灌溉方案(50%、75%和100%植物需水量)为主小区，蚯蚓堆肥V (0.5 t ha-1) × γ氨基丁酸GABA水平(0,5、10 mg l-1)为副小区。严重干旱胁迫对株高影响显著。与对照条件相比，使用50%压水堆后植株高度降低了31.8%。虽然干旱胁迫对种子质量和产量有负面影响，但叶面施用GABA可以缓解这些影响。施用50%压水堆后，10 mg l-1 GABA比无叶处理种子产量和收获指数分别提高21.22%和15.5%。压水比从100%降低到50%，导致磷、钾浓度增加，糖和脯氨酸含量增加。在相同条件下，使用GABA或V对这些性状的改善效果显著。在用水效率方面也记录了类似的趋势。因此，使用10 mg l-1的GABA和5 t ha-1的V可以有效缓解水分胁迫。
{"title":"Evaluation of yield and water use efficiency of quinoa under irrigation regimes, gamma aminobutyric acid, and vermicompost application","authors":"Mehdi Jamshideyni, M. Behdani, S. Parsa, S. Khoramdel","doi":"10.14720/aas.2023.119.1.2970","DOIUrl":"https://doi.org/10.14720/aas.2023.119.1.2970","url":null,"abstract":"The current study was aim to evaluate the interaction effects of gamma aminobutyric acid (GABA) and vermicompost on yield and yield components of quinoa under different levels of drought stress. For this, two experiments were similarly designed as the factorial-split with four replicates for evaluating this hypothesis. Irrigation regimes (50, 75, and 100 % of plant water requirement (PWR)) as the main plot and vermicompost V (0, 5 t ha-1) × gamma aminobutyric acid GABA levels (0, 5, 10 mg l-1) as the subplot were designed. Severe drought stress had a significant effect on plant height. Plant height reduced 31.8 % after using 50 % of PWR compared to the control conditions. Although drought stress negatively affected the 1000 seed mass and seed yield, GABA foliar application alleviated these effects. After using 50 % of PWR, 10 mg l-1 of GABA increased the seed yield and harvest index up to 21.22 and 15.5 %, respectively, compared to the non-foliar application. The reduction in PWR from 100 to 50 % led to increasing in P and K concentrations, as well as sugar and proline contents. In the same conditions, the use of GABA or V had a significant effect on improving these traits. A similar trend was also recorded in relation to water use efficiency. Therefore, using 10 mg l-1 of GABA and 5 t ha-1 of V can be effective in alleviating water stress.","PeriodicalId":6884,"journal":{"name":"Acta agriculturae Slovenica","volume":"63 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84723465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-28DOI: 10.14720/aas.2023.119.1.2764
Sergey Сhetverikov, A. Feoktistova, M. Timergalin, T. Rameev, Gaisar Hkudaygulov, Aliya Kendjieva, M. Bakaeva, Darya Сhetverikova, Sergey Starikov, D. Sharipov
Effect of auxin-producing bacterial strain (Pseudomonas protegens DA1.2) was investigated under conditions of drought and herbicide treatment in wheat plants. Positive effect of the bacterial suspension on wheat plants treated with auxinic herbicide under drought conditions was manifested in reducing the content of malondialdehyde and proline, preventing inhibition of plant growth and normalizing chlorophyll content. Under combined stress, changes in concentrations and redistribution of phytohormones in plants were detected. An imbalance in auxin distribution between shoots and roots could be the reason for the decrease in plant resistance to drought in combination with the herbicide. Treatment of plants with the bacterial suspension restored normal shoot-to-root ratio of auxins in plants. Thus, this bacterial strain showed the properties of synthetic auxin antidotes and can be recommended for optimizing the technology of herbicide application under drought conditions.
{"title":"Mitigation of the negative effect of auxinic herbicide by bacterial suspension of Pseudomonas protegens DA1.2 in wheat plants under drought conditions","authors":"Sergey Сhetverikov, A. Feoktistova, M. Timergalin, T. Rameev, Gaisar Hkudaygulov, Aliya Kendjieva, M. Bakaeva, Darya Сhetverikova, Sergey Starikov, D. Sharipov","doi":"10.14720/aas.2023.119.1.2764","DOIUrl":"https://doi.org/10.14720/aas.2023.119.1.2764","url":null,"abstract":"Effect of auxin-producing bacterial strain (Pseudomonas protegens DA1.2) was investigated under conditions of drought and herbicide treatment in wheat plants. Positive effect of the bacterial suspension on wheat plants treated with auxinic herbicide under drought conditions was manifested in reducing the content of malondialdehyde and proline, preventing inhibition of plant growth and normalizing chlorophyll content. Under combined stress, changes in concentrations and redistribution of phytohormones in plants were detected. An imbalance in auxin distribution between shoots and roots could be the reason for the decrease in plant resistance to drought in combination with the herbicide. Treatment of plants with the bacterial suspension restored normal shoot-to-root ratio of auxins in plants. Thus, this bacterial strain showed the properties of synthetic auxin antidotes and can be recommended for optimizing the technology of herbicide application under drought conditions.","PeriodicalId":6884,"journal":{"name":"Acta agriculturae Slovenica","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84113393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-28DOI: 10.14720/aas.2023.119.1.2953
Saša Gačnik, Stanislav Trdan, Maja MIKULIČ-PETKOVŠEK
Global food production is forced to search for new approaches to protect plants from harmful organisms and environmental factors. One of the alternatives could be the use of salicylic acid (SA) and its derivatives. Overall, the effects of SA at the primary ecosystem level are encouraging, contributing to improved productivity and quality of many plants and improving tolerance to many stressors. The secondary level of effects of SA in the environment represents the effects on harmful organisms due to direct action and also the indirect effects of SA that occur due to morphological and physiological changes when the plant adapts to stressors. In many cases, SA has the effect of reducing infections, and it also acts as a deterrent to some pests. After being attacked by a pest, plants release volatile compounds into the environment, mainly SA derivatives such as methylated SA (MeSA). This attracts the natural enemies of pests, which could be used to protect plants from pests, as MeSA has been found to act as an attractant in many species. Salicylates have a very wide spectrum of action, which trigger various effects in the environment, which intertwine with each other and consequently affect several levels in the exosystem. In this article, we divided the effects of salicylates according to different levels in the environment, which gave us a broader insight into the potential use of salicylates in agriculture.
{"title":"Vplivi salicilne kisline in njenih derivatov na rastline, škodljive in koristne organizme in njihove interakcije v okolju","authors":"Saša Gačnik, Stanislav Trdan, Maja MIKULIČ-PETKOVŠEK","doi":"10.14720/aas.2023.119.1.2953","DOIUrl":"https://doi.org/10.14720/aas.2023.119.1.2953","url":null,"abstract":"Global food production is forced to search for new approaches to protect plants from harmful organisms and environmental factors. One of the alternatives could be the use of salicylic acid (SA) and its derivatives. Overall, the effects of SA at the primary ecosystem level are encouraging, contributing to improved productivity and quality of many plants and improving tolerance to many stressors. The secondary level of effects of SA in the environment represents the effects on harmful organisms due to direct action and also the indirect effects of SA that occur due to morphological and physiological changes when the plant adapts to stressors. In many cases, SA has the effect of reducing infections, and it also acts as a deterrent to some pests. After being attacked by a pest, plants release volatile compounds into the environment, mainly SA derivatives such as methylated SA (MeSA). This attracts the natural enemies of pests, which could be used to protect plants from pests, as MeSA has been found to act as an attractant in many species. Salicylates have a very wide spectrum of action, which trigger various effects in the environment, which intertwine with each other and consequently affect several levels in the exosystem. In this article, we divided the effects of salicylates according to different levels in the environment, which gave us a broader insight into the potential use of salicylates in agriculture.","PeriodicalId":6884,"journal":{"name":"Acta agriculturae Slovenica","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76296248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-28DOI: 10.14720/aas.2023.119.1.2564
H. Sammama, Mohamed Najib Alfeddy, D. Hsissou, Mimoun El Kaoua
Intercropping has proved to be a promising alternative in the biological control of biotic factors by reducing the excessive use of plant protection products that are harmful to the environment and human health. In this study, aimed to examinate the effect of intercropping systems on diseases, weeds and pests control in organic field experiments in Western Morocco. Two field experiments were conducted during 2017-2018 and 2018-2019. Three cropping regimes (monocropped wheat, monocropped faba bean, and intercropped wheat-faba bean) and three nitrogen levels N0 (0 kg N ha-1), N1 (50 kg N ha-1), and N2 (100 kg N ha-1) were evaluated. Compared with monocropping, intercropping (N0 level) reduced the incidence of stripe rust by 71–120 % and severity by 244–337 % in 1st and 2nd experiments respectively. In addition, the incidence of septoria was reduced by 236 % and severity by 276 %. Obviously, the intercrops significantly decreased the total weed biomass by more than 40 % in both experiments. Black aphid populations in faba bean were reduced by 80 %. In contrast, the nitrogen fertilizer increased the attack of diseases and black aphids. It is concluded that wheat-faba bean intercrops can be used as a method of reduction of inputs, reduction of environmental impacts of crops, and stability in the face of biotic factors.
间作通过减少对环境和人类健康有害的植物保护产品的过度使用，已被证明是生物控制生物因素的一种有前途的替代方法。本研究旨在考察摩洛哥西部有机大田试验中间作制度对病虫害防治的影响。在2017-2018年和2018-2019年分别进行了两次田间试验。试验评价了3种种植制度(单作小麦、单作蚕豆和间作小麦-蚕豆)和3个氮肥水平(N0 (0 kg N ha-1)、N1 (50 kg N ha-1)和N2 (100 kg N ha-1)。与单作相比，间作(no水平)在第1次和第2次试验中，条锈病发生率分别降低71 ~ 120%，严重程度分别降低244 ~ 337%。此外，室间隔的发生率降低了236%，严重程度降低了276%。在两个试验中，间作显著降低了杂草总生物量，降幅均在40%以上。蚕豆黑蚜种群减少80%。相反，施氮肥增加了病害和黑蚜的侵袭。综上所述，小麦-蚕豆间作可以作为一种减少投入、减少作物对环境影响、在生物因素面前保持稳定性的方法。
{"title":"Potential effect of intercropping in the control of weeds, diseases, and pests in a wheat-faba bean system","authors":"H. Sammama, Mohamed Najib Alfeddy, D. Hsissou, Mimoun El Kaoua","doi":"10.14720/aas.2023.119.1.2564","DOIUrl":"https://doi.org/10.14720/aas.2023.119.1.2564","url":null,"abstract":"Intercropping has proved to be a promising alternative in the biological control of biotic factors by reducing the excessive use of plant protection products that are harmful to the environment and human health. In this study, aimed to examinate the effect of intercropping systems on diseases, weeds and pests control in organic field experiments in Western Morocco. Two field experiments were conducted during 2017-2018 and 2018-2019. Three cropping regimes (monocropped wheat, monocropped faba bean, and intercropped wheat-faba bean) and three nitrogen levels N0 (0 kg N ha-1), N1 (50 kg N ha-1), and N2 (100 kg N ha-1) were evaluated. Compared with monocropping, intercropping (N0 level) reduced the incidence of stripe rust by 71–120 % and severity by 244–337 % in 1st and 2nd experiments respectively. In addition, the incidence of septoria was reduced by 236 % and severity by 276 %. Obviously, the intercrops significantly decreased the total weed biomass by more than 40 % in both experiments. Black aphid populations in faba bean were reduced by 80 %. In contrast, the nitrogen fertilizer increased the attack of diseases and black aphids. It is concluded that wheat-faba bean intercrops can be used as a method of reduction of inputs, reduction of environmental impacts of crops, and stability in the face of biotic factors.","PeriodicalId":6884,"journal":{"name":"Acta agriculturae Slovenica","volume":"57 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81627663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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