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EFFECTS OF DIFFERENT PHOSPHORUS IMMOBILIZATION WAYS ON PHOSPHORUS SORPTION BEHAVIORS IN SEDIMENT OF LAKE CHAOHU: EFFECTS OF DIFFERENT PHOSPHORUS IMMOBILIZATION WAYS ON PHOSPHORUS SORPTION BEHAVIORS IN SEDIMENT OF LAKE CHAOHU 不同磷固定方式对巢湖湖沉积物磷吸附行为的影响:不同磷固定方式对巢湖湖沉积物磷吸附行为的影响
Pub Date : 2011-04-28 DOI: 10.3724/SP.J.1035.2011.00319
Yunbing Liu, A. Sun, Xiuyun Cao, Chunlei Song, Yiyong Zhou
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引用次数: 2
SNPS AND THEIR APPLICATIONS IN STUDIES ON GENETICS AND BREEDING OF AQUACULTURE ANIMALS: SNPS AND THEIR APPLICATIONS IN STUDIES ON GENETICS AND BREEDING OF AQUACULTURE ANIMALS SNPS及其在水产动物遗传育种研究中的应用:SNPS及其在水产动物遗传育种研究中的应用
Pub Date : 2011-04-28 DOI: 10.3724/SP.J.1035.2011.00348
X. Tan, J. Tong
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引用次数: 0
PCR AMPLIFICATION, SEQUENCING AND ANALYSIS OF 18S rDNA OF BALANTIDIUM CTENOPHARYNGODONI INHABITING GRASS CARP: PCR AMPLIFICATION, SEQUENCING AND ANALYSIS OF 18S rDNA OF BALANTIDIUM CTENOPHARYNGODONI INHABITING GRASS CARP 草鱼中棘球蚴18S rDNA的PCR扩增、测序和分析:草鱼中棘球蚴18S rDNA的PCR扩增、测序和分析
Pub Date : 2011-04-27 DOI: 10.3724/SP.J.1035.2011.00203
Ming Li, Chong Wang, Jianguo Wang, Da Yu, Wen-bo Wang, X. Ge, P. Xu, Jun Xie, Xiaoning Gong
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引用次数: 3
ISOLATION, TRANSCRIPTS POLYMORPHISM AND TISSUES EXPRESSION OF GROWTH HORMONE RECEPTOR GENES IN CYPRINUS CARPIO VAR. JIAN: ISOLATION, TRANSCRIPTS POLYMORPHISM AND TISSUES EXPRESSION OF GROWTH HORMONE RECEPTOR GENES IN CYPRINUS CARPIO VAR. JIAN 鲤生长激素受体基因的分离、转录本多态性及组织表达:鲤生长激素受体基因的分离、转录本多态性及组织表达
Pub Date : 2011-04-27 DOI: 10.3724/SP.J.1035.2011.00218
Ju-hua Yu, Hongxia Li, Yongkai Tang, Jian-lin Li, Z. Dong
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引用次数: 1
PRELIMINARY STUDIES ON THE CORRELATION BETWEEN MICROSATELLITE MARKERS AND GROWTH TRAITS IN CHINESE MITTEN CRAB ( ERIOCHEIR SINENSIS ): PRELIMINARY STUDIES ON THE CORRELATION BETWEEN MICROSATELLITE MARKERS AND GROWTH TRAITS IN CHINESE MITTEN CRAB ( ERIOCHEIR SINENSIS ) 中华绒螯蟹微卫星标记与生长性状相关性的初步研究:中华绒螯蟹微卫星标记与生长性状相关性的初步研究
Pub Date : 2011-04-27 DOI: 10.3724/SP.J.1035.2011.00197
Yan Wu, Chunpeng Fu, Sufei Jiang, H. Fu, H. Qiao, Y. Gong, Y. Xiong
{"title":"PRELIMINARY STUDIES ON THE CORRELATION BETWEEN MICROSATELLITE MARKERS AND GROWTH TRAITS IN CHINESE MITTEN CRAB ( ERIOCHEIR SINENSIS ): PRELIMINARY STUDIES ON THE CORRELATION BETWEEN MICROSATELLITE MARKERS AND GROWTH TRAITS IN CHINESE MITTEN CRAB ( ERIOCHEIR SINENSIS )","authors":"Yan Wu, Chunpeng Fu, Sufei Jiang, H. Fu, H. Qiao, Y. Gong, Y. Xiong","doi":"10.3724/SP.J.1035.2011.00197","DOIUrl":"https://doi.org/10.3724/SP.J.1035.2011.00197","url":null,"abstract":"","PeriodicalId":6937,"journal":{"name":"水生生物学报","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2011-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70058943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
CLONING AND EXPRESSION ANALYSIS OF MOLT-INHIBITING HORMONE GENE ( Es-MIH ) IN ERIOCHEIR SINENSIS : CLONING AND EXPRESSION ANALYSIS OF MOLT-INHIBITING HORMONE GENE ( Es-MIH ) IN ERIOCHEIR SINENSIS 中华绒螯蟹蜕皮抑制激素基因(Es-MIH)的克隆与表达分析:中华绒螯蟹蜕皮抑制激素基因(Es-MIH)的克隆与表达分析
Pub Date : 2011-04-27 DOI: 10.3724/SP.J.1035.2011.00210
Yan Sun, Yichen Zhang, Yichen Liu, Xuehui Wang, Yufan Wang, Xuyun Geng, Jinsheng Sun, Wei-Jun Yang
{"title":"CLONING AND EXPRESSION ANALYSIS OF MOLT-INHIBITING HORMONE GENE ( Es-MIH ) IN ERIOCHEIR SINENSIS : CLONING AND EXPRESSION ANALYSIS OF MOLT-INHIBITING HORMONE GENE ( Es-MIH ) IN ERIOCHEIR SINENSIS","authors":"Yan Sun, Yichen Zhang, Yichen Liu, Xuehui Wang, Yufan Wang, Xuyun Geng, Jinsheng Sun, Wei-Jun Yang","doi":"10.3724/SP.J.1035.2011.00210","DOIUrl":"https://doi.org/10.3724/SP.J.1035.2011.00210","url":null,"abstract":"","PeriodicalId":6937,"journal":{"name":"水生生物学报","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2011-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70058599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
OBSERVATION ON THE DEVELOPMENT OF THE LATERAL LINE NEUROMAST OF SIBERIAN STURGEON(ACIPENSER BAERII) 西伯利亚鲟侧线神经肥大发育的观察
Pub Date : 2011-01-01 DOI: 10.3724/sp.j.1035.2011.00115
C. Qian
Acipenseriformes fish is a kind of the ancient chondrichthyes fish,and Siberian sturgeon(Acipenser baerii) plays an important part in the evolutionary history of vertebrates.Lateral line neuromast is the key part of the lateral line system-a very important sense organ of fish.Therefore,it is necessary to pay attention to the development of the lat-eral line neuromast of the Siberian sturgeon.In order to observing the morphology and tracking the development of lateral line neuromast of the Siberian sturgeon larvae in the different development periods after hatching.The 5μm of the paraffin sections of the adult fish of the Siberian sturgeon were prepared and then stained with HE.Meanwhile,the Siberian sturgeon larvae in different periods after hatching stained with 10μmol/L DiA [4-(4-Diethylaminostyryl)-1-methylpyridinium iodide] which can be used as the neuromast specific markers.The results showed morphology of the neuromast in the lateral line canal: The hair cells erected and arranged closely.Compared to Hammerhead shark(Sphyma lewini),Japanese eel(Lyncozymba nystromi) and Tongue sole(Cynoglossus semilaevis),all of these species had many common characteristics: The neuromast included epithelial cells,hair cells and supporting cells.However,each of these species had special characteristics: The Siberian sturgeon had smaller amount of the hair cells;the Ham-merhead shark had larger amount of the epithelial cells;the Japanese eel had many vacuoles in the supporting cells and the neuromast of the Tongue sole exposed to the skin surface.At the same time,the development of the lateral line neuromast of the Siberian sturgeon had been tracked: 3 days after hatching,the ganglia had dramatic differentiation and development in the region of the ear;10 days after hatching,the ganglia in the region of the ear had differentiation and formed lateral line neuromast above and below the orbital and also began formatting the lateral line neuromast on the opercular;15 days after hatching,the lateral line neuromast above and below the orbital became more obvious,and the lateral line neuromast on the trunk had basically developed;Finally,it is observed that the lateral line neuromast of the Siberian sturgeon larvae had developed completely in 22 days after hatching.Compared to Zebrafish(Danio rerio) and Catshark(Scyliorhinuscanicula),the development process of the lateral line neuromast of all these species were nearly the same.This study may lay morphologic foundation for the further researching on the related genes which express in the development of the lateral line neuromast of the Siberian sturgeon as well as provide useful information for the fur-ther studying on the migration of cells in the development of the lateral line neuromast of the Siberian sturgeon.
鲟目鱼类是一种古老的软骨鱼类鱼类,西伯利亚鲟在脊椎动物进化史上占有重要地位。侧线神经杆是鱼类重要的感觉器官侧线系统的关键部分。因此,有必要重视西伯利亚鲟鱼侧线神经肥大的发育。为了观察西伯利亚鲟鱼幼鱼在孵化后不同发育时期的侧线神经肥大形态和发育情况。制作西伯利亚鲟鱼成鱼的5μm石蜡切片,用HE染色。同时,用10μmol/L的DiA[4-(4-二乙基氨基苯乙烯基)-1-甲基碘化吡啶]染色,可作为神经肥大特异性标记物。结果显示:侧线管神经肥大形态:毛细胞竖立,排列紧密。与锤头鲨(Sphyma lewini)、日本鳗鲡(Lyncozymba nystromi)和舌鳎(Cynoglossus semilaevis)相比,这些物种都具有许多共同的特征:神经肥大包括上皮细胞、毛细胞和支持细胞。然而,这些物种中的每一个都有自己的特点:西伯利亚鲟鱼的毛细胞数量较少;锤头鲨的上皮细胞数量较多;日本鳗鱼的支持细胞中有许多液泡,舌底的神经肥大暴露在皮肤表面。与此同时,对西伯利亚鲟鱼侧线神经肥大的发育进行了跟踪:孵化后第3天,耳区神经节分化发育明显;孵化后第10天,耳区神经节分化形成眶上、眶下侧线神经杆,并开始形成眼上侧线神经杆;孵化后第15天,眶上、眶下侧线神经杆更加明显,躯干侧线神经杆基本发育观察到西伯利亚鲟鱼幼鱼的侧线神经肥大在孵化后22天发育完全。与斑马鱼(Danio rerio)和猫鲨(Scyliorhinuscanicula)相比,这些物种的侧线神经肥大的发育过程几乎相同。本研究可为进一步研究西伯利亚鲟鱼侧线神经肥大发育过程中表达的相关基因奠定形态学基础,并为进一步研究西伯利亚鲟鱼侧线神经肥大发育过程中细胞的迁移提供有用信息。
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引用次数: 0
KARYOTYPE ANALYSIS OF THE FRESHWATER DUGESIA PLANARIANS FROM YUNNAN PROVINCE,CHINA 云南淡水涡虫的核型分析
Pub Date : 2011-01-01 DOI: 10.3724/sp.j.1035.2011.00333
Chen Guang
In order to provide evidence for taxonomy,evolutionary biology,biogeography and phylogenetics of the ge-nus Dugesia,the chromosomes and karyotypes of the freshwater planarians,Dugesia sp.,collected from Shuhe town in Lijiang City,Longwangtang pond in Baoshan City,and Xiaozhaongdian town in Xianggelila City,Yunnan Province,were studied.The adult planarians,15—20 mm in length and 1.5—2.0 mm in width,were used.After starving for a week,each planarian was transversally cut into five pieces,the planarian pieces were cultured in distilled water for three or four days to regenerate.For the karyological analysis,the metaphase chromosomes were prepared from regenerating blastemas by an air-drying method.Chromosome numbers of over 100 well-spreaded cells were counted and the chro-mosomes were photographed using Leica DMLB light microscope.The karyotype parameters of five well-spreaded metaphase plates from five individuals were measured.Relative length(r.l.) was calculated as chromosome length ×100/ total length of haploid genome.Centromeric index(c.i.) was calculated as length of the short arm×100/ total length of the chromosome.Arm ratio(a.r.) was calculated as length of the long arm/that of the short arm.Mean values and standard deviations(S.D.) were calculated from five metaphase plates obtained from a minimum of five fissiparous specimens from each experimental population.The karyotype were arranged firstly according to the gradually decreasing lengths of chromosomes and then the centromeric indices.The chromosomes were classified based on the criteria pro-posed by Levan,et al(1964).The results showed that planarians collected from Shuhe town had a diploid chromosome number of 16 with a karyotype formula of 2n=2x=16=16m.Planarians collected from Longwangtang pond had both the triploid 24(2n=3x=24=24m) and partial diploid 16(2n=2x=16=16m),i.e,which were mixoploids.It was noteworthy that the planarians collected from Xiaozhaongdian town had a triploid aneuploid chromosome number of 26 with a karyotype formula of 2n=3x+2=24+2=21m+3st+2m,but a few were 25(2n=3x+1=24+1=21m+3st+1m) or 24(2n=3x=24=21m+3st).The karyological results of the planarians collected from Xiaozhaongdian town were obviously different from those of Dugesia japonica and other species in Dugesia,The main factor that affected the karyological results might be the change of environment(altitude,water temperature,atmospheric pressure and UV radiation).Per-haps it was the change of chromosomes and karyotypes that led to speciation and the evolution of species.Based on the results of karyotype analysis of freshwater planarians,Dugesia sp.,from the three places,classification of freshwater planarians and chromosome aneuploidy had been discussed in this paper.In order to obtain more precise karyological data of Dugesia planarian,other sensitive techniques such as chromosome banding,fluorescent staining or in situ hy-bridization should be useful to get more evidence for taxonomy,genetics,evoluti
为了为杜氏属(genus Dugesia)的分类、进化生物学、生物地理学和系统发育提供依据,对云南省丽江市树河镇、宝山市龙王塘塘和香格里拉市小昭店镇的淡水涡虫杜氏属(Dugesia sp.)的染色体和核型进行了研究。采用长15 ~ 20mm,宽1.5 ~ 2.0 mm的成虫。饥饿一周后,将每只涡虫横切成5块,在蒸馏水中培养3 - 4天,使其再生。为进行核学分析,采用风干法制备了再生胚的中期染色体。利用徕卡DMLB光镜对100多个分布均匀的细胞进行染色体计数,并对染色体进行拍照。测定了来自5个个体的5个伸展良好的中期板的核型参数。相对长度(r.l.)计算为染色体长度×100/单倍体基因组总长度。着丝粒指数(c.i.)计算为短长度arm×100/染色体总长度。臂比(a.r.)计算为长臂的长度/短臂的长度。从每个实验群体中至少五个分裂标本中获得五个中期板,计算平均值和标准差(sd)。核型首先按照染色体长度逐渐减小的顺序排列,然后按照着丝粒指数排列。根据Levan等人(1964)提出的标准对染色体进行分类。结果表明,树河镇涡虫的二倍体染色体数为16,核型公式为2n=2x=16=16m。龙王塘纯涡虫既有三倍体24(2n=3x=24=24m),也有部分二倍体16(2n=2x=16=16m)。E,它们是混合倍体。值得注意的是,小肇店镇采得的涡虫染色体数目为26条三倍体非整倍体,核型公式为2n=3x+2=24+2=21m+3st+2m,但也有少数为25条(2n=3x+1=24+1=21m+3st+1m)或24条(2n=3x=24=21m+3st)。小昭店镇采得的涡虫的核生理结果与日本杜鹃及杜鹃属其他物种有明显差异,影响其核生理结果的主要因素可能是环境(海拔、水温、气压和紫外线辐射)的变化。也许是染色体和核型的改变导致了物种形成和物种进化。本文根据三地淡水涡虫Dugesia sp.的核型分析结果,讨论了淡水涡虫的分类和染色体非整倍性。为了获得更精确的全涡虫核生物学资料,需要利用染色体显带、荧光染色或原位杂交等敏感技术,为涡虫的分类、遗传学、进化生物学、生物地理学和生理遗传学等方面提供更多的证据。
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引用次数: 1
RIPARIAN PLANTS IN THE MAINSTREAM OF THE YELLOW RIVER:ASSEMBLAGE CHARACTERISTICS AND ITS INFLUENCING FACTORS 黄河干流河岸植物:群落特征及其影响因素
Pub Date : 2011-01-01 DOI: 10.3724/sp.j.1035.2011.00051
She Ya
Riparian vegetation,as a key component of riparian ecosystem,offers suitable habitat and migrating or dif-fusing corridor for animals and plants,and has an important function in buffering and filtrating non-point pollution.The Yellow River is an important wetland in China,and also an essential zone supporting biodiversity.However,the devel-opment of riparian vegetation along the Yellow River is threatened by flow regime alteration caused by the overexploi-tation of water resources such as agricultural irrigation and hydraulic engineering construction.Embankment along the lower river is also destroying the habitats of the riparian vegetation.However,previous researches on the riparian vege-tation along the Yellow River focused on only some specific area.To ensure a deep understanding of the present status of the riparian vegetation and provide support for the protection of riparian vegetation diversity and the riparian man-agement of the Yellow River,two systematic surveys were carried out in April to June and September to October,2008,respectively.The species composition and species diversity of riparian plant communities were analyzed.Classifications of the vegetation by two way indicator species analysis(TWINSPAN) were also carried out.Altogether 169 species belonging to 37 families and 124 genera were recorded.Floristic analysis showed that the flora had diverse geographical origins.With regard to the spatial distribution,the herbaceous plants in the middle river sections were more abundant than those in the upper and lower river sections both in biomass and density.TWINSPAN classification divided the vegetation into 17 communities.The characteristics of the communities were described.The correlative analyses indi-cated that altitude,annual mean temperature,annual mean rainfall,annual mean runoff,mean maximum velocity were the main factors influencing the structure and distribution of the assemblages.
河岸植被作为河岸生态系统的重要组成部分,为动植物提供了适宜的栖息地和迁移或扩散通道,具有缓冲和过滤非点源污染的重要功能。黄河是中国重要的湿地,也是支持生物多样性的重要地带。然而,由于农业灌溉和水利工程建设等水资源过度开发导致的流态改变,黄河沿岸植被的发展受到威胁。下游河堤也在破坏河岸植被的栖息地。然而,以往对黄河河岸植被的研究主要集中在特定区域。为深入了解黄河河岸植被现状,为黄河河岸植被多样性保护和河岸管理提供依据,于2008年4 - 6月和9 - 10月进行了两次系统调查。分析了滨水植物群落的物种组成和物种多样性。利用双向指示种分析法(TWINSPAN)对植被进行了分类。共记录到37科124属169种。植物区系分析表明,该植物区系具有不同的地理来源。从空间分布上看,中游草本植物的生物量和密度均高于上、下游草本植物。TWINSPAN分类将植被分为17个群落。描述了群落的特征。相关分析表明,海拔高度、年平均气温、年平均降雨量、年平均径流量、年平均最大流速是影响群落结构和分布的主要因素。
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引用次数: 2
EFFECTS OF DIETARY LIPID LEVELS ON GROWTH PERFORMANCE,NUTRIENT DIGESTIBILITY AND BLOOD BIOCHEMICAL INDICES OF GIFT TILAPIA(OREOCHROMIS NILOTICUS) 饲料脂肪水平对馈赠罗非鱼生长性能、营养物质消化率和血液生化指标的影响
Pub Date : 2011-01-01 DOI: 10.3724/sp.j.1035.2011.00080
W. Ai
In order to determine the optimal levels of the lipid of GIFT strain Nile tilapia(Oreochromis niloticus),630 GIFT with average weight of(2.63±0.16) g were divided into six groups(1.73% lipid,3.71% lipid,5.69% lipid,7.67% lipid,9.64% lipid and 16.55% lipid) randomly.One with triplication was the control group fed with basal diet(1.73% lipid),the others were the treated groups fed with basal diet(1.73% lipid) supplemented with 2%,4%,6%,8% and 15% fish oil,respectively.After rearing 90d,the growth,feed conversion ratio,nutrient apparent digestibility and blood bio-chemical parameter were determined.Results showed that,along with dietary lipid level raising,weight gain rate and specific growth rate had a trend of going up first then falling down(P0.05),protein efficiency ratio improved(P0.01) and feed conversion ratio reduced(P0.01).Second-order regression of weight gain rate on concentrations of dietary lipid indicated that the optimal dietary lipid for maximal growth of GIFT tilapia was about 9.34%.Crude protein and dry-matter digestibility had no significant effected by dietary lipid levels(P0.05).Crude lipid and phosphorus digesti-bility raised significantly with dietary lipid level increasing(P0.05).Albumin and albumin/globulin ratio in 1.73% group(this group did not added fish oil) was significantly higher than other group(P0.05).Along with dietary lipid level raising,cholesterol and alkaline phosphatase of fish serum raised significantly(P0.01).Dietary lipid level had significantly influence on blood glucose(P0.05),and there was no significantly influence on the content of triglyceride and the activities of glutamic-pyruvic transaminase and glutamic-oxaloacetic transaminase(P0.05).It indicated that the level of lipid in the dietary could improve the growth,the apparent digestibilities of crude lipid and phosphorus to some degree,but excessive amount of lipid content in the dietary may be negative effects of fish growth and immune ability in GIFT.So 7.67%-9.34% lipid of the dietary are the optimal levels of GIFT Seedlings in aquaculture.
为确定GIFT品系尼罗罗非鱼(Oreochromis niloticus)的最佳脂质水平,将630尾平均体重为(2.63±0.16)g的GIFT鱼随机分为6组(1.73%脂质、3.71%脂质、5.69%脂质、7.67%脂质、9.64%脂质和16.55%脂质)。对照组饲喂基础饲粮(脂肪含量为1.73%),试验组分别饲喂在基础饲粮(脂肪含量为1.73%)中添加2%、4%、6%、8%和15%鱼油的试验组。饲养90d后,测定生长、饲料系数、营养物质表观消化率和血液生化指标。结果表明:随着饲粮脂肪水平的升高,增重率和特定生长率呈先上升后下降的趋势(P0.05),蛋白质效率提高(P0.01),饲料系数降低(P0.01)。体重增加率对饲料脂肪浓度的二阶回归表明,GIFT罗非鱼最大生长所需的饲料脂肪浓度为9.34%左右。饲粮脂肪水平对粗蛋白质和干物质消化率无显著影响(P0.05)。随着饲粮脂肪水平的升高,粗脂肪和磷消化率显著升高(P0.05)。1.73%组(不添加鱼油)的白蛋白和白蛋白/球蛋白比显著高于其他组(P0.05)。随着饲粮脂肪水平的升高,鱼血清胆固醇和碱性磷酸酶显著升高(P0.01)。饲粮脂肪水平对血糖有显著影响(P0.05),对甘油三酯含量、谷丙转氨酶和谷草酰转氨酶活性无显著影响(P0.05)。综上所述,饲料中脂肪含量在一定程度上促进了GIFT鱼的生长,提高了粗脂肪和磷的表观消化率,但饲料中脂肪含量过高可能会对GIFT鱼的生长和免疫能力产生负面影响。因此,饲料中脂肪含量为7.67% ~ 9.34%为GIFT苗水产养殖的最佳水平。
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引用次数: 14
期刊
水生生物学报
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