Pub Date : 2011-01-01DOI: 10.3724/sp.j.1035.2011.00300
W. Gao
In order to determine the interaction between nutrient concentration and simulated microgravity(SMG),Microcystis aeruginosa,cultured under rich and low nutrient medium(BG-11 and 1/10 BG-11 medium),were treated with different gravity conditions.Both of M.aeruginosa in BG-11 and 1/10 BG-11 were sensitive to SMG.Their growth and metabolism changed significantly after 2day-treatments.The effects of SMG varied significantly with the nutrient concentrations of medium.For M.aeruginosa cultured in BG-11,SMG led to the decreased cell number and photochemical efficiency of PS II as well as the increased cellular concentrations of chlorophyll a,carotenoid,phycocyanin,soluble protein and microcystin.The SMG induced changes of cells in 1/10 BG-11 were contrary to the above-mentioned changes of cells in BG-11.In1/10 BG-11,SMG led to the increased cell number and photochemical efficiency of PS II as well as the decreased cellular concentrations of chlorophyll a,carotenoid,phycocyanin,soluble protein and micro-cystin.The effects of SMG and nutrient limitation were alike when they were applied to M.aeruginosa separately.The influences of nutrient limitation were stronger than those of SMG.Under SMG,maybe the nutrient demand of cells was reduced,as a result of the suppressed growth and photochemical activity.Otherwise,SMG might enable cells to use nutriments more effectively.Thus,SMG could alleviate the stress of low nutrient medium to M.aeruginosa.Collectively,these findings supported that the nutrient concentration of medium could significantly affect the responses of M.aeruginosa to SMG.
{"title":"THE EFFECTS OF SIMULATED MICROGRAVITY ON MICROCYSTIS AERUGINOSA DEPENDING ON THE NUTRIENT CONCENTRATIONS OF MEDIUM","authors":"W. Gao","doi":"10.3724/sp.j.1035.2011.00300","DOIUrl":"https://doi.org/10.3724/sp.j.1035.2011.00300","url":null,"abstract":"In order to determine the interaction between nutrient concentration and simulated microgravity(SMG),Microcystis aeruginosa,cultured under rich and low nutrient medium(BG-11 and 1/10 BG-11 medium),were treated with different gravity conditions.Both of M.aeruginosa in BG-11 and 1/10 BG-11 were sensitive to SMG.Their growth and metabolism changed significantly after 2day-treatments.The effects of SMG varied significantly with the nutrient concentrations of medium.For M.aeruginosa cultured in BG-11,SMG led to the decreased cell number and photochemical efficiency of PS II as well as the increased cellular concentrations of chlorophyll a,carotenoid,phycocyanin,soluble protein and microcystin.The SMG induced changes of cells in 1/10 BG-11 were contrary to the above-mentioned changes of cells in BG-11.In1/10 BG-11,SMG led to the increased cell number and photochemical efficiency of PS II as well as the decreased cellular concentrations of chlorophyll a,carotenoid,phycocyanin,soluble protein and micro-cystin.The effects of SMG and nutrient limitation were alike when they were applied to M.aeruginosa separately.The influences of nutrient limitation were stronger than those of SMG.Under SMG,maybe the nutrient demand of cells was reduced,as a result of the suppressed growth and photochemical activity.Otherwise,SMG might enable cells to use nutriments more effectively.Thus,SMG could alleviate the stress of low nutrient medium to M.aeruginosa.Collectively,these findings supported that the nutrient concentration of medium could significantly affect the responses of M.aeruginosa to SMG.","PeriodicalId":6937,"journal":{"name":"水生生物学报","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2011-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70059143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2011-01-01DOI: 10.3724/sp.j.1035.2011.00246
Z. Qiu
In order to basically figure out the metabolism and demand on the fatty acids of rice field eel,Monopterus albus Zuiew,and provide a frame of reference on selective using different lipid sources for rice diets production of field eel,six isonitrogenous(44% crude protein) and isoenergetic(19.4 MJ/kg) experimental diets were formulated by using six different lipid sources:fish oil(FO),soybean oil(SO),and linseed oil(LO),ARA + peanut oil(APO),peanut oil(PO) and pork lard(PL).Female rice field eels(initial weight of 15.5 g) were fed with these diets for 10 weeks.The fats and fatty acid compositions in the muscle liver and ovary of these eels were analyzed by means of chloroform-methanol and gas chromatography(GC) and the growth performance were evaluated with the parameter of specific growth rate(SGR).The results showed that there were effects of different lipid sources on the growth performance of rice field eels.The SGR of rice field eels fed with PO without n-3 polyunsaturated fatty acids(PUFAs) was significantly lower than those fed with SO and FO(P0.05);the specific growth rate(SGR) of rice field eels fed with SO was significantly higher than those fed with LO,APO,PO and PL,except FO(P0.05).There were positive linear correlation between the content both 18:3n-3 and 18:2n-6 in the various tissues of rice field eels and those in experimental diets.When the experiment diets were lack of eicosapentaenoic acid(EPA,20:5n-3) and docosahexaenoic acid(DHA,22:6n-3),there were positive logarithmic correlation between the content of EPA,DHA in ovaries and the content of 18:3n-3 in diets;while the ex-perimental diets were lack of Arachidonic acid(ARA,20:4n-6),there were positive linear correlation between the con-tent of ARA in muscle,liver and ovary and the content of 18:2n-6 in diets.The content of EPA and DHA in the various tissues of the rice field eels fed with diet(FO) with EPA and DHA were significantly higher than those of the rice field eels fed diets(LO,SO,PL,PO) without EPA and DHA(P0.05).In general,the n-3PUFAs and appropriate n-6/n-3 ratio were important for the growth of rice field eels.Soybean oil,linseed oil and pork lard were feasible lipid resources for replace fish oil on growth of rice field eels and soybean oil is preferable lipid resources.The fatty acid(18:2n-6,18:3n-3,ARA,EPA and DHA) profiles of liver,muscle and ovary reflected profiles of the corresponding dietary lipid sources.The rice field eel only possess the infirmatory ability to synthesize EPA and DHA from linolenic acid(18:3n-3) and ARA from linoleic acid(18:2n-6) for the limitation in the capacity of fatty acid desaturation.
{"title":"EFFECTS OF DIETARY LIPID SOURCES ON TISSUE FATTY ACIDS PROFILE ANDGROWTH PERFORMANCE IN FEMALE RICE FIELD EEL MONOPTERUS ALBUS,ZUIEW","authors":"Z. Qiu","doi":"10.3724/sp.j.1035.2011.00246","DOIUrl":"https://doi.org/10.3724/sp.j.1035.2011.00246","url":null,"abstract":"In order to basically figure out the metabolism and demand on the fatty acids of rice field eel,Monopterus albus Zuiew,and provide a frame of reference on selective using different lipid sources for rice diets production of field eel,six isonitrogenous(44% crude protein) and isoenergetic(19.4 MJ/kg) experimental diets were formulated by using six different lipid sources:fish oil(FO),soybean oil(SO),and linseed oil(LO),ARA + peanut oil(APO),peanut oil(PO) and pork lard(PL).Female rice field eels(initial weight of 15.5 g) were fed with these diets for 10 weeks.The fats and fatty acid compositions in the muscle liver and ovary of these eels were analyzed by means of chloroform-methanol and gas chromatography(GC) and the growth performance were evaluated with the parameter of specific growth rate(SGR).The results showed that there were effects of different lipid sources on the growth performance of rice field eels.The SGR of rice field eels fed with PO without n-3 polyunsaturated fatty acids(PUFAs) was significantly lower than those fed with SO and FO(P0.05);the specific growth rate(SGR) of rice field eels fed with SO was significantly higher than those fed with LO,APO,PO and PL,except FO(P0.05).There were positive linear correlation between the content both 18:3n-3 and 18:2n-6 in the various tissues of rice field eels and those in experimental diets.When the experiment diets were lack of eicosapentaenoic acid(EPA,20:5n-3) and docosahexaenoic acid(DHA,22:6n-3),there were positive logarithmic correlation between the content of EPA,DHA in ovaries and the content of 18:3n-3 in diets;while the ex-perimental diets were lack of Arachidonic acid(ARA,20:4n-6),there were positive linear correlation between the con-tent of ARA in muscle,liver and ovary and the content of 18:2n-6 in diets.The content of EPA and DHA in the various tissues of the rice field eels fed with diet(FO) with EPA and DHA were significantly higher than those of the rice field eels fed diets(LO,SO,PL,PO) without EPA and DHA(P0.05).In general,the n-3PUFAs and appropriate n-6/n-3 ratio were important for the growth of rice field eels.Soybean oil,linseed oil and pork lard were feasible lipid resources for replace fish oil on growth of rice field eels and soybean oil is preferable lipid resources.The fatty acid(18:2n-6,18:3n-3,ARA,EPA and DHA) profiles of liver,muscle and ovary reflected profiles of the corresponding dietary lipid sources.The rice field eel only possess the infirmatory ability to synthesize EPA and DHA from linolenic acid(18:3n-3) and ARA from linoleic acid(18:2n-6) for the limitation in the capacity of fatty acid desaturation.","PeriodicalId":6937,"journal":{"name":"水生生物学报","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2011-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70058879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2011-01-01DOI: 10.3724/sp.j.1035.2011.00360
Zuo Ming
{"title":"THE VARIATION OF PHYTOPLANKTON COMMUNITY STRUCTURE IN WINTER AND SPRING IN LAKE SHAHU AT WUHAN","authors":"Zuo Ming","doi":"10.3724/sp.j.1035.2011.00360","DOIUrl":"https://doi.org/10.3724/sp.j.1035.2011.00360","url":null,"abstract":"","PeriodicalId":6937,"journal":{"name":"水生生物学报","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2011-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70059359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2011-01-01DOI: 10.3724/sp.j.1035.2011.00180
Huang Pu
{"title":"MICROCYSTIN-LR INDUCES PHOSPHORYLATION OF ERK AND JNK IN HUMAN HEPATOCYTE HL7702","authors":"Huang Pu","doi":"10.3724/sp.j.1035.2011.00180","DOIUrl":"https://doi.org/10.3724/sp.j.1035.2011.00180","url":null,"abstract":"","PeriodicalId":6937,"journal":{"name":"水生生物学报","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2011-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70058809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2011-01-01DOI: 10.3724/sp.j.1035.2011.00276
Z. Ling
The effects of short-term acute temperature change(20℃→10℃ and 20℃→30℃) and long-term temperature acclimation(10,20 and 30℃,two weeks) on resting oxygen consumption rate(Resting oxygen consumption rate,VO2) and critical swimming speed(Critical swimming speed,Ucrit) of juvenile southern catfish(Silurus meridionalis Chen) were conducted to examine the different physiological responses of fish underwent acute and chronic temperature change.The results of present study showed that the relative critical swimming speeds(Relative critical swimming speed,Ur) of 10,20 and 30 ℃ acclimation group were 1.83,2.87 and 3.37 BL/s(Body length per second)(P0.05),respectively.The Ur of both acute temperature elevation group and acute temperature decline group was not significantly different from that of corresponding acclimation group(P0.05).The relationship between Ur and acclimation temperature showed as Ur =0.8114T+ 1.0976(n=37,R2 = 0.973,P0.05).The VO2 increased profoundly with temperature(P0.05) and those values were 14.91,28.34 and 44.98 mg O2/kg·h,respectively.Both acute high temperature exposure and acute cold temperature exposure have a significant influence on VO2 of juvenile southern catfish.The VO2 of acute temperature elevation group firstly increased and then decreased slowly to a relative stable VO2.The peak value(3.1) of Q10 of VO2 in high temperature exposure group appeared at 0.5h after acute temperature change and was about two folds that of control group while the peak value(3.1) of Q10 of VO2 in acute temperature decline group appeared at 8h after acute temperature change and was about four folds that of control group.It suggested that acute temperature change exerted a profoundly influence on the metabolic physiological process of juvenile southern catfish.The metabolic responses to acute temperature decline group differed significantly from that of acute temperature elevation group.The physiological stress elicited by acute temperature decline might be much higher than that of acute temperature elevation group.When faced the acute change of environmental temperature,juvenile southern catfish can compensate for the physical negative effect of acute temperature change on swimming performance physiologically.Fish might adjust the metabolism strategy to meet the energy demand of locomotion when necessary and hence increase the fitness of fish during an acute temperature descendent adversity.
{"title":"EFFECTS OF ACUTE TEMPERATURE CHANGE ON RESTING OXYGEN CONSUMPTION RATE AND CRITICAL SWIMMING SPEED IN JUVENILE SOUTHERN CATFISH (SILURUS MERIDIONALIS CHEN)","authors":"Z. Ling","doi":"10.3724/sp.j.1035.2011.00276","DOIUrl":"https://doi.org/10.3724/sp.j.1035.2011.00276","url":null,"abstract":"The effects of short-term acute temperature change(20℃→10℃ and 20℃→30℃) and long-term temperature acclimation(10,20 and 30℃,two weeks) on resting oxygen consumption rate(Resting oxygen consumption rate,VO2) and critical swimming speed(Critical swimming speed,Ucrit) of juvenile southern catfish(Silurus meridionalis Chen) were conducted to examine the different physiological responses of fish underwent acute and chronic temperature change.The results of present study showed that the relative critical swimming speeds(Relative critical swimming speed,Ur) of 10,20 and 30 ℃ acclimation group were 1.83,2.87 and 3.37 BL/s(Body length per second)(P0.05),respectively.The Ur of both acute temperature elevation group and acute temperature decline group was not significantly different from that of corresponding acclimation group(P0.05).The relationship between Ur and acclimation temperature showed as Ur =0.8114T+ 1.0976(n=37,R2 = 0.973,P0.05).The VO2 increased profoundly with temperature(P0.05) and those values were 14.91,28.34 and 44.98 mg O2/kg·h,respectively.Both acute high temperature exposure and acute cold temperature exposure have a significant influence on VO2 of juvenile southern catfish.The VO2 of acute temperature elevation group firstly increased and then decreased slowly to a relative stable VO2.The peak value(3.1) of Q10 of VO2 in high temperature exposure group appeared at 0.5h after acute temperature change and was about two folds that of control group while the peak value(3.1) of Q10 of VO2 in acute temperature decline group appeared at 8h after acute temperature change and was about four folds that of control group.It suggested that acute temperature change exerted a profoundly influence on the metabolic physiological process of juvenile southern catfish.The metabolic responses to acute temperature decline group differed significantly from that of acute temperature elevation group.The physiological stress elicited by acute temperature decline might be much higher than that of acute temperature elevation group.When faced the acute change of environmental temperature,juvenile southern catfish can compensate for the physical negative effect of acute temperature change on swimming performance physiologically.Fish might adjust the metabolism strategy to meet the energy demand of locomotion when necessary and hence increase the fitness of fish during an acute temperature descendent adversity.","PeriodicalId":6937,"journal":{"name":"水生生物学报","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2011-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70059041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2011-01-01DOI: 10.3724/sp.j.1035.2011.00238
Zhang De, Pr Chin
The Aphanizomenon flos-aquae DC-1,frequently appeares in freshwater,is a dominant species in Dianchi Lake of Yunnan Province,China,in winter and spring.During the past 20 years,blooms of Aphanizomenon flos-aquae DC-1 have occurred in the Dianchi Lake per year because of heavy pollution and the dominant species overgrowth.The blooms always sustained for six months in a year,even throughout a year in some water of Dianchi Lake.And the blooms could also give off distasteful smell into the air.As a result,visitors are more likely to breathe the odorous air than fresh air today,and see a bad scenery of green-mud water than clear blue waters.Especially the dominant species can produce aphantoxins,which is attributed to the paralytic shellfish poisons(PSPs) and influences aquatic ecosystem and damages the nerve system of animals and human.However,little research has been carried out on the toxin,in particular the effects on central nervous system(CNS) of animals and human.Thus the present research is to investigate abnormity of apoptotic gene expression,ultrastructural damage of brain induced by aphantoxin,to reveal mechanism of response of brain on zebrafish to aphantoxins.Virus-free male zebrafish(Danio rerio),110 days old or so,average bodyweight of(0.4±0.01) g,after 7—10 days of acclimatization,were randomly assigned into control and treated groups(45 fish per group).The two groups were received 30μL 0.01 mol/L acetic acid solution(control) and 30 μL 0.01 mol/L acetic acid solution contained aphantoxins(5.3 μg STXeq/kg bodyweight,treated) through intraperitoneal injections(i.p.).At each time point(1,3,6,9,12 and 24h),five zebrafish in each group were sacrificed by cold shock(embedding into crushed ice at -8℃),and cerebra were removed from whole brain and washed at once in 0.86% ice-cold physiological saline,prefixed in 2.5% glutaraldehyde solution for electron microscopy.In another 5 fish at each timepoint,whole brains were removed as described above,frozen in liquid nitrogen,and stored at either -70℃ for subsequent RNA analysis.The prefixed cerebra(at 9h) were dehydrated in graded ethanol and propylene oxide,embedded in Epon 812,and cut into the ultra-thin sections using glass knives on an ultramicrotome(Leica,Germany).Sections were stained with uranyl acetate and lead citrate before electron microscopy(JEM-1230,Japan).The 45mg frozen(-70 ℃) whole brain(five fish each timepoint) of zebrafish were homogenized(IKA Werke,Germany) in ice-cold TRIzoL reagent(w:v,1:20,30s) to extract total RNA.Gene-specific RNA levels were measured using an RT-PCR kit(Promega,USA) according to the protocol of manufacturer.The system of reactions was carried out into 50μL as follows:Reactions contained 25μL of 2× AccessQuick TM Master Mix,5 μL oligo-dT,10 μmol/L upstream and downstream primers,1 μg template RNA,and RNase-free MilliQ water to 50μL.AMV reverse transcriptase(1μL) was added and incubated at 45℃ for 45 min for reverse transcription.Samples were then heated at
{"title":"RESPONSE OF APOPTOTIC GENES TO APHANTOXIN-PARALYTIC SHELLFISH POISON IN FRESHWATER EXTRACTED FROM THE APHANIZOMENON FLOS-AQUAE DC-1 IN CELLS OF BRAIN ON ZEBRAFISH (DANIO RERIO)","authors":"Zhang De, Pr Chin","doi":"10.3724/sp.j.1035.2011.00238","DOIUrl":"https://doi.org/10.3724/sp.j.1035.2011.00238","url":null,"abstract":"The Aphanizomenon flos-aquae DC-1,frequently appeares in freshwater,is a dominant species in Dianchi Lake of Yunnan Province,China,in winter and spring.During the past 20 years,blooms of Aphanizomenon flos-aquae DC-1 have occurred in the Dianchi Lake per year because of heavy pollution and the dominant species overgrowth.The blooms always sustained for six months in a year,even throughout a year in some water of Dianchi Lake.And the blooms could also give off distasteful smell into the air.As a result,visitors are more likely to breathe the odorous air than fresh air today,and see a bad scenery of green-mud water than clear blue waters.Especially the dominant species can produce aphantoxins,which is attributed to the paralytic shellfish poisons(PSPs) and influences aquatic ecosystem and damages the nerve system of animals and human.However,little research has been carried out on the toxin,in particular the effects on central nervous system(CNS) of animals and human.Thus the present research is to investigate abnormity of apoptotic gene expression,ultrastructural damage of brain induced by aphantoxin,to reveal mechanism of response of brain on zebrafish to aphantoxins.Virus-free male zebrafish(Danio rerio),110 days old or so,average bodyweight of(0.4±0.01) g,after 7—10 days of acclimatization,were randomly assigned into control and treated groups(45 fish per group).The two groups were received 30μL 0.01 mol/L acetic acid solution(control) and 30 μL 0.01 mol/L acetic acid solution contained aphantoxins(5.3 μg STXeq/kg bodyweight,treated) through intraperitoneal injections(i.p.).At each time point(1,3,6,9,12 and 24h),five zebrafish in each group were sacrificed by cold shock(embedding into crushed ice at -8℃),and cerebra were removed from whole brain and washed at once in 0.86% ice-cold physiological saline,prefixed in 2.5% glutaraldehyde solution for electron microscopy.In another 5 fish at each timepoint,whole brains were removed as described above,frozen in liquid nitrogen,and stored at either -70℃ for subsequent RNA analysis.The prefixed cerebra(at 9h) were dehydrated in graded ethanol and propylene oxide,embedded in Epon 812,and cut into the ultra-thin sections using glass knives on an ultramicrotome(Leica,Germany).Sections were stained with uranyl acetate and lead citrate before electron microscopy(JEM-1230,Japan).The 45mg frozen(-70 ℃) whole brain(five fish each timepoint) of zebrafish were homogenized(IKA Werke,Germany) in ice-cold TRIzoL reagent(w:v,1:20,30s) to extract total RNA.Gene-specific RNA levels were measured using an RT-PCR kit(Promega,USA) according to the protocol of manufacturer.The system of reactions was carried out into 50μL as follows:Reactions contained 25μL of 2× AccessQuick TM Master Mix,5 μL oligo-dT,10 μmol/L upstream and downstream primers,1 μg template RNA,and RNase-free MilliQ water to 50μL.AMV reverse transcriptase(1μL) was added and incubated at 45℃ for 45 min for reverse transcription.Samples were then heated at ","PeriodicalId":6937,"journal":{"name":"水生生物学报","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2011-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70058832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2011-01-01DOI: 10.3724/sp.j.1035.2011.00313
L. Jing
Microcystins(MCs) are hepatotoxins which inhibit the activity of protein phosphatase 2A(PP2A) notably.The cells were incubated with different concentrations of MC-LR to determine the effect of MCs on PP2A in HL7702 respectively for 24h.The mRNA and protein levels of PP2A subunits were detected.Furthermore,the activity of PP2A was assayed after 3h,6h,12h and 24h exposure.The protein levels were not changed significantly except B55α which decreased concentration-dependently and significantly in 1000 nmol/L.For mRNA levels,15 subunits increased in 100 nmol/L group,whereas 7 subunits in 500 nmol/L group and 6 subunits in 1000 nmol/L group decreased.The activity of PP2A was inhibited significantly and concentration-dependently after 6h exposure while there were no changes in 3,12 and 24h exposure.In conclusion,MC-LR affects the transcription of PP2A subunits after 24h exposure,without concomitant changes in the interrelated proteins.Exposure time might be a determinant when considering the effect of MC-LR on PP2A activity.
{"title":"EFFECT OF MICROCYSTIN LR ON PP2A IN HL7702 CELL","authors":"L. Jing","doi":"10.3724/sp.j.1035.2011.00313","DOIUrl":"https://doi.org/10.3724/sp.j.1035.2011.00313","url":null,"abstract":"Microcystins(MCs) are hepatotoxins which inhibit the activity of protein phosphatase 2A(PP2A) notably.The cells were incubated with different concentrations of MC-LR to determine the effect of MCs on PP2A in HL7702 respectively for 24h.The mRNA and protein levels of PP2A subunits were detected.Furthermore,the activity of PP2A was assayed after 3h,6h,12h and 24h exposure.The protein levels were not changed significantly except B55α which decreased concentration-dependently and significantly in 1000 nmol/L.For mRNA levels,15 subunits increased in 100 nmol/L group,whereas 7 subunits in 500 nmol/L group and 6 subunits in 1000 nmol/L group decreased.The activity of PP2A was inhibited significantly and concentration-dependently after 6h exposure while there were no changes in 3,12 and 24h exposure.In conclusion,MC-LR affects the transcription of PP2A subunits after 24h exposure,without concomitant changes in the interrelated proteins.Exposure time might be a determinant when considering the effect of MC-LR on PP2A activity.","PeriodicalId":6937,"journal":{"name":"水生生物学报","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2011-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70059266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2011-01-01DOI: 10.3724/sp.j.1035.2011.00355
Z. Jun
{"title":"GENE EXPRESSION AND THYROID HORMONE REGULATED TRANSCRIPT OF IGF-I DURING METAMORPHOSIS OF THE FLOUNDER,PARALICHTHYS OLIVACEUS","authors":"Z. Jun","doi":"10.3724/sp.j.1035.2011.00355","DOIUrl":"https://doi.org/10.3724/sp.j.1035.2011.00355","url":null,"abstract":"","PeriodicalId":6937,"journal":{"name":"水生生物学报","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2011-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70059232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2010-12-24DOI: 10.3724/SP.J.1035.2010.01211
Mingzheng Li, Wei-Chuan Jiang, Xin Gao, Z. Duan, Huanzhang Liu
{"title":"STATUS QUO OF EARLY LIFE HISTORY STAGES AT WUXUE CROSS-SECTION OF THE YANGTZE RIVER: STATUS QUO OF EARLY LIFE HISTORY STAGES AT WUXUE CROSS-SECTION OF THE YANGTZE RIVER","authors":"Mingzheng Li, Wei-Chuan Jiang, Xin Gao, Z. Duan, Huanzhang Liu","doi":"10.3724/SP.J.1035.2010.01211","DOIUrl":"https://doi.org/10.3724/SP.J.1035.2010.01211","url":null,"abstract":"","PeriodicalId":6937,"journal":{"name":"水生生物学报","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2010-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70057174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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