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猪源乳酸杆菌与益生元组合筛选及其体外发酵特性 猪源乳酸杆菌与益生元组合筛选及其体外发酵特性
Pub Date : 2020-06-08 DOI: 10.13343/j.cnki.wsxb.20200102
朱晓峰 | 张桢 | 崔雷鸿 | 朱伟云 | 杭苏琴
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引用次数: 0
高效组成型分泌表达木素过氧化物酶酿酒酵母工程菌的构建 高效组成型分泌表达木素过氧化物酶酿酒酵母工程菌的构建
Pub Date : 2020-01-21 DOI: 10.13343/j.cnki.wsxb.20190373
肖建龙 | 张斯童 | 孙晓仲 | 陈光
{"title":"高效组成型分泌表达木素过氧化物酶酿酒酵母工程菌的构建","authors":"肖建龙 | 张斯童 | 孙晓仲 | 陈光","doi":"10.13343/j.cnki.wsxb.20190373","DOIUrl":"https://doi.org/10.13343/j.cnki.wsxb.20190373","url":null,"abstract":"","PeriodicalId":7120,"journal":{"name":"微生物学报","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142059145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
有氧条件下污染禾谷镰刀菌的玉米品质变化规律和呕吐毒素的积累动态变化规律 有氧条件下污染禾谷镰刀菌的玉米品质变化规律和呕吐毒素的积累动态变化规律
Pub Date : 2020-01-17 DOI: 10.13343/j.cnki.wsxb.20190514
闫珊珊 | 刘光芒 | 王建萍 | 林燕 | 白世平 | 张克英 | 吴彩梅
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引用次数: 0
[Antibacterial activity of silver nanoparticles against multiple drug resistant strains]. [银纳米颗粒对多种耐药菌株的抑菌活性]。
Pub Date : 2017-04-04
Xueqing Chen, Jiaxuan Jiang, Zhihong Ren, Juan Li, Hongying Zhang, Jianguo Xu, Huamao Du

Objective: The objective of the study was to assess the antimicrobial activity of silver nanoparticles (AgNPs) against multiple drug resistant strains.

Methods: Minimal inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of AgNPs against three model microbes, namely Escherichia coli, Staphylococcus aureus, Candida albicans were measured by microdilution broth method. Time-kill curve within 24 h was made according to colony count method after three model microbes were treated with a series concentration of AgNPs. Post-antibiotic effect was tested by colony count method. Finally, we determined the antimicrobial efficacy against multiple drug resistant strains in biological safety laboratory grade 2 (BSL-2).

Results: AgNPs with a diameter of 5 nm to 30 nm were synthesized by the biological method. The zeta potential was -19.5 mV. The time-kill curve of the three model microbes showed time-dependent antibacterial activity. The effect of AgNPs on E. coli and C. albicans after "antibiotic effect" increased with time, there was no obvious "post-antibiotic effect" on S. aureus. Both MIC values and MBC values of AgNPs for the three model microbes were between 1 μg/mL and 4 μg/mL. However, the MIC value of AgNPs for the three human multidrug-resistant strains was 6 μg/mL to 26 μg/mL and MBC value of AgNPs was 10 μg/mL to 32 μg/mL. The MIC values of AgNPs for 14 animal multi-drug resistant strains were between 4 μg/mL and 10 μg/mL, and the MBC values were between 8 μg/mL and 16 μg/mL. The MBC/MIC values of all the tested strains were less than 2.

Conclusion: AgNPs is a time-dependent antimicrobial agent with different "post-antibiotic effect", which can inhibit both human and animal-derived multi-drug resistant bacteria.

目的:研究银纳米颗粒(AgNPs)对多种耐药菌株的抑菌活性。方法:采用微量稀释肉汤法测定AgNPs对大肠杆菌、金黄色葡萄球菌、白色念珠菌3种模式微生物的最低抑菌浓度(MIC)和最低杀菌浓度(MBC)。用系列浓度AgNPs处理3种模型微生物后,按菌落计数法绘制24h内的时间杀伤曲线。采用菌落计数法检测抗菌后效果。最后,在生物安全二级实验室(BSL-2)中测定其对多重耐药菌株的抗菌效果。结果:采用生物法制备了直径为5 ~ 30 nm的AgNPs。zeta电位为-19.5 mV。三种模式微生物的抑菌时间曲线均表现出时间依赖性。AgNPs对大肠杆菌和白色念珠菌的“抗生素效应”后作用随时间增加,对金黄色葡萄球菌无明显的“抗生素后效应”。3种模式微生物AgNPs的MIC值和MBC值均在1 ~ 4 μg/mL之间。AgNPs对3株人多药耐药菌株的MIC值为6 ~ 26 μg/mL, MBC值为10 ~ 32 μg/mL。14株动物多重耐药菌株的AgNPs MIC值在4 ~ 10 μg/mL之间,MBC值在8 ~ 16 μg/mL之间。所有菌株的MBC/MIC值均小于2。结论:AgNPs是一种具有不同“抗生素后效应”的时间依赖性抗菌药物,对人源性和动物源性多重耐药菌均有抑制作用。
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引用次数: 0
[Distribution, structure and sequence alignment, and metagenomics analysis of two nitrite reductases with NO forming]. [两种亚硝酸盐还原酶的分布、结构和序列比对及宏基因组学分析]。
Pub Date : 2017-04-04
Yufeng Xin, Tianying Zhao, Xiaohua Qu

Objective: To reflect the importance of nitrite reductase (NIR) in the environment, we studied its distribution.

Methods: The sequences of NIR were searched in the sequenced genome database at NCBI based on previous reported NIR sequences. The sequence similarity was done by multiple sequence alignment, and phylogenetic relationship was evaluated via constructing the phylogenetic tree. Furthermore, their distribution in the marine metagenome was studied by metagenomics.

Results: The homologues of these two enzymes were 397 and 812 strains in sequenced genome, and the proportion was 8 and 15.7 percent, respectively. Almost all of archaea containing type II NIR. They have high identity by multiple sequence alignment analysis. The cofactor, the substrate and the cooper binding sites in type II were high conserved, suggesting that these enzymes had the specific function in denitrification. Phylogenetic analysis showed the two enzymes may have the common ancestor. In marine metagenome analysis, type I and II have 6 and 35 reads per 100000 reads, respectively, the two types of NIRs have the biggest proportion at the tropical south pacific area.

Conclusion: Collectively, we suggested NIR, especially type II, play a key role in bioremediation of nitrogen contamination.

目的:研究亚硝酸盐还原酶(NIR)在环境中的分布,以反映其在环境中的重要性。方法:根据已有报道的NIR序列,在NCBI测序基因组数据库中检索NIR序列。通过多序列比对确定序列相似性,构建系统发育树评价系统发育关系。利用宏基因组学研究了它们在海洋宏基因组中的分布。结果:两种酶在测序基因组中同源菌株分别为397株和812株,所占比例分别为8%和15.7%。几乎所有的古菌都含有II型近红外光谱。经多序列比对分析,它们具有较高的同源性。II型酶的辅因子、底物和铜结合位点高度保守,表明这些酶在反硝化过程中具有特定的功能。系统发育分析表明,这两种酶可能具有共同的祖先。在海洋宏基因组分析中,I型和II型的近红外光谱分别为6和35 / 100000 reads,这两种近红外光谱在热带南太平洋地区所占比例最大。结论:综上所述,NIR,尤其是II型,在氮污染的生物修复中起着关键作用。
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引用次数: 0
[Phylogenetic and genetic heterogeneity of 23 Acidithiobacillus strains isolated from different geographical locations]. [不同地理位置分离的23株酸性硫杆菌的系统发育和遗传异质性]。
Pub Date : 2017-04-04
Pengwu Lin, Minrui Liu, Min Zhang, Aiying Wang, Yongqing Ni

Objective: To study the phylogenetic and genetic heterogeneity of 23 Acidithiobacillus strains from various geographical locations, as well as the relationship between the DNA fingerprinting classification and geographical origin of Acidithiobacillus.

Methods: Partial 16S-23S rRNA gene intergenic spacer (ITS) was used to construct corresponding phylogenetic trees based on the sequence homology. rus gene amplification and rep-PCR assay with two different primers (BOXAIR and ERIC) were performed to analyze genetic heterogeneity of Acidithiobacillus strains from diverse environment.

Results: Acidithiobacillus revealed a great genetic heterogeneity. The whole isolates were classified into five groups by ITS sequence analysis. This result was similar with that obtained by rep-PCR. Acidithiobacillus ferrooxidans strains were always divided into two groups of phylogenetic and BOXAIR fingerprinting cluster analysis. However, these were clustered one group in the ERIC dendrogram. Genotypic analysis of the rus gene suggested that different iron oxidation pathways have been evolved in these closely related bacteria. Taken together, the iron oxidation pathway of Acidithiobacillus and phylogenetic groups have no obvious correlation. ITS gene has been proven very useful in distinguishing closely related species or subspecies of Acidithiobacillus, to BOXAIR-PCR, which has been recommended as reliable tool for genetic heterogeneity analysis of Acidithiobacillus.

目的:研究不同地理位置的23株酸性硫杆菌的系统发育和遗传异质性,以及DNA指纹图谱分类与酸性硫杆菌地理来源的关系。方法:采用部分16S-23S rRNA基因间间隔(ITS)基于序列同源性构建相应的系统发育树。采用两种不同引物(BOXAIR和ERIC)对不同环境下的酸性硫杆菌进行基因扩增和rep-PCR分析。结果:酸性硫杆菌具有很强的遗传异质性。通过ITS序列分析将整个菌株分为5个类群。该结果与rep-PCR结果相似。酸性氧化亚铁硫杆菌的系统发育和BOXAIR指纹聚类分析均将菌株分为两类。然而,这些都聚集在ERIC树形图中的一个组。rus基因的基因型分析表明,这些密切相关的细菌已经进化出不同的铁氧化途径。综上所述,酸性硫杆菌的铁氧化途径与系统发育类群无明显相关性。ITS基因已被证明对酸性硫杆菌的近缘种或亚种的区分非常有用,BOXAIR-PCR已被推荐为酸性硫杆菌遗传异质性分析的可靠工具。
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引用次数: 0
[Diversity of microbial community structure in the spermosphere of saline-alkali soil in shandong area]. [山东地区盐碱地土壤中微生物群落结构多样性]。
Pub Date : 2017-04-04
Zhimeng Zhang, Dunwei Ci, Guanchu Zhang, Hong Ding, Jishun Yang, Liangxiang Dai, Dai Zhang

Objective: Three soil types in different salt contents were taken as the experiment objectives. We evaluated the effect of various saline alkali soil types on diversity of bacterial community structure in spermosphere soil during water absorption and germination of peanut seeds.

Methods: The V3-V4 region of 16S ribosomal RNA genes was amplified using PCR, and the PCR products were then analyzed using Illumina high-throughput sequencing technology.

Results: (1) The diversity of soil bacterial community in saline alkali soil was higher than that in non-saline alkali soil. Especially, the highest diversity was in spermosphere soil from Qingtuo. (2) The microflora structures in different soils were distinct at the class level. Soil bacteria in four samples were classified into six classes, including Proteobacteria, Actinobacteria, Actinobacteria, Bacteroidetes, Acidobacteria and Firmicutes. Proteobacteria and Actinobacteria groups were dominant in colonies. The analysis of whole samples colony structure showed that the difference of type and abundance at phylum and genus level during different adsorption time was most significant (P<0.05). (3) The analysis of beta diversity and phylogenetic distances of constructed phylogenetic trees revealed that the sequenced clones fell into two major groups within the domain bacteria.

Conclusion: The diversity of bacteria community compositions in the high salt content soil was higher. There were obvious differences in microbial community structure of different soil types at class level, primarily in the Proteobacteria and Actinobacteria. The type and abundance of microbial colonies at both phylum and genus levels were affected by the seed germination time. However, there was no influence on the genetic distance between the samples from the same soil type.

目的:以3种不同含盐量的土壤类型为试验对象。研究了不同盐碱土类型对花生种子吸水和萌发过程中精子圈土壤细菌群落结构多样性的影响。方法:采用PCR扩增16S核糖体RNA基因v3 ~ v4区,PCR产物采用Illumina高通量测序技术进行分析。结果:(1)盐碱地土壤细菌群落多样性高于非盐碱地。其中,青坨的气圈土壤多样性最高。(2)不同土壤的微生物区系结构在类水平上存在差异。将4个样品的土壤细菌分为变形菌门(Proteobacteria)、放线菌门(Actinobacteria)、放线菌门(Actinobacteria)、拟杆菌门(Bacteroidetes)、酸性菌门(Acidobacteria)和厚壁菌门(Firmicutes) 6类。菌落以变形菌群和放线菌群为主。对全样品菌落结构的分析表明,不同吸附时间在门、属水平上的类型和丰度差异最为显著(p)。结论:高含盐量土壤中细菌群落组成的多样性更高。不同土壤类型的微生物群落结构在纲水平上存在明显差异,以变形菌门和放线菌门为主。门和属水平上的微生物菌落类型和丰度均受种子萌发时间的影响。同一土壤类型样品间的遗传距离不受影响。
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引用次数: 0
[Putative regulatory protein STM14_3514 decreases Salmonella Typhimurium invasion of epithelial cells]. [推测的调节蛋白STM14_3514减少鼠伤寒沙门氏菌侵袭上皮细胞]。
Pub Date : 2017-04-04
Lingyan Jiang, Qixing Zhou, Peisheng Wang, Xiaohan Jiang, Lu Feng

Objective: To study the function and mechanism of STM14_3514 gene that encoded in Salmonella pathogenicity island (SPI)-1 of Salmonella enterica serovar Typhimurium strain ATCC 14028.

Methods: We constructed STM14_3514 mutant strain and a complemented strain of the mutant. Through mice experiment, attachment assays, invasion assays, macrophage replication assays, western blot, and Quantitative real-time PCR analysis (qRT-PCR), we compared the virulence of the mutant strain to that of the wild-type 14028.

Results: STM14_3514 mutant shows increased virulence to mice, and the bacterial number of STM14_3514 mutant in liver, spleen, and ileum was more abundant than that of the wild-type strain. The increased virulence of STM14_3514 mutant is caused by its elevated invasion ability to epithelial cells (>2-fold and P<0.05). qRT-PCR and western blot results show that STM14_3514 reduced the expression of HilA and another SPI-1invasion locus. Moreover, the repression of HilA by STM14_3514 is mediated by HilC.

Conclusion: STM14_3514 is a negative regulator in SPI-1, which can repress HilA and SPI-1invasion locus through HilC, and possibly contribute to the repression on SPI-1 after bacterial invasion.

目的:研究肠沙门氏菌血清型鼠伤寒杆菌ATCC 14028沙门氏菌致病性岛(SPI)-1中编码的STM14_3514基因的功能及作用机制。方法:构建STM14_3514突变株及其互补株。通过小鼠实验、附着实验、侵袭实验、巨噬细胞复制实验、western blot和实时荧光定量PCR (Quantitative real-time PCR)分析,我们比较了突变株与野生型14028的毒力。结果:STM14_3514突变株对小鼠的毒力增强,其在肝脏、脾脏和回肠中的细菌数量比野生型菌株丰富。STM14_3514突变体毒力的增强是由于其对上皮细胞的侵袭能力提高(>2倍)所致。结论:STM14_3514是SPI-1的负调控因子,可通过HilC抑制HilA和SPI-1的侵袭位点,可能与细菌侵袭后SPI-1的抑制有关。
{"title":"[Putative regulatory protein STM14_3514 decreases Salmonella Typhimurium invasion of epithelial cells].","authors":"Lingyan Jiang,&nbsp;Qixing Zhou,&nbsp;Peisheng Wang,&nbsp;Xiaohan Jiang,&nbsp;Lu Feng","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>To study the function and mechanism of STM14_3514 gene that encoded in Salmonella pathogenicity island (SPI)-1 of Salmonella enterica serovar Typhimurium strain ATCC 14028.</p><p><strong>Methods: </strong>We constructed STM14_3514 mutant strain and a complemented strain of the mutant. Through mice experiment, attachment assays, invasion assays, macrophage replication assays, western blot, and Quantitative real-time PCR analysis (qRT-PCR), we compared the virulence of the mutant strain to that of the wild-type 14028.</p><p><strong>Results: </strong>STM14_3514 mutant shows increased virulence to mice, and the bacterial number of STM14_3514 mutant in liver, spleen, and ileum was more abundant than that of the wild-type strain. The increased virulence of STM14_3514 mutant is caused by its elevated invasion ability to epithelial cells (>2-fold and P<0.05). qRT-PCR and western blot results show that STM14_3514 reduced the expression of HilA and another SPI-1invasion locus. Moreover, the repression of HilA by STM14_3514 is mediated by HilC.</p><p><strong>Conclusion: </strong>STM14_3514 is a negative regulator in SPI-1, which can repress HilA and SPI-1invasion locus through HilC, and possibly contribute to the repression on SPI-1 after bacterial invasion.</p>","PeriodicalId":7120,"journal":{"name":"微生物学报","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36095181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Two sample pooling strategies revealed different root-associated fungal diversity of Rhododendron species]. [两种样本池策略揭示了不同杜鹃花物种根相关真菌多样性]。
Pub Date : 2017-04-04
Caiwei Huang, Yinghui Liao, Qiong Ding

Objective: ooling of multiple samples is widely used in studying general patterns of microbial communities that are heterogeneously structured in space. Pooling strategies and the number of sequence reads generate biases in the description of diversity and community structure of root-associated fungi. Therefore, we developed a molecular toolbox for fast and accurate identification of the root-associated fungal community of Rododendron species.

Methods: Multiple root samples of R. lutescens and R. bureavii were collected for DNA extraction. Effects of two different pooling strategies, i.e. pooling samples prior to vs. post PCR, on fungal species composition were studied by comparing results within host species.

Results: Species richness and Shannon-Wiener index of fungal communities of clone library constructed by pooling samples after PCR were higher than that of pooling prior to PCR. High frequency fungal species were detected by both pooling strategies, whereas infrequent species detected by the two strategies differed. Notably, the prior to PCR pooling strategy effectively alleviated the unwanted amplification of host plant sequences when fungal specific primer ITS1f and ITS4 were used. Accumulation curves of fungal species suggested that sequencing at least 50 clones can fully reflect species composition of clone library of the two Rhododendron root-associated fungal community.

Conclusion: Clone library constructed by post PCR pooling of samples is better in providing accurate views of fungal diversity and community structure of Rhododendron species.

目的:多样品冷却被广泛应用于研究空间异质性结构的微生物群落的一般模式。池化策略和序列读取数在描述根相关真菌的多样性和群落结构时会产生偏差。因此,我们开发了一个分子工具箱,用于快速准确地鉴定杜鹃属植物的根相关真菌群落。方法:采集多根木犀草和布氏木犀草进行DNA提取。通过比较宿主物种内的结果,研究了两种不同的池化策略(即PCR前和PCR后的池化策略)对真菌物种组成的影响。结果:PCR后池化构建的克隆文库真菌群落的物种丰富度和Shannon-Wiener指数均高于PCR前池化构建的克隆文库。两种方法均能检测到高频真菌种类,而两种方法检测到的低频率真菌种类不同。值得注意的是,当使用真菌特异性引物ITS1f和ITS4时,预先PCR池策略有效地减轻了寄主植物序列的不必要扩增。真菌物种积累曲线表明,至少50个克隆的测序可以充分反映两个杜鹃根相关真菌群落克隆文库的物种组成。结论:采用聚合酶链反应(post - PCR)方法构建的克隆文库能够较准确地了解杜鹃属植物的真菌多样性和群落结构。
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引用次数: 0
[Effects of glucose on photosynthesis and growth of Chloralla sp. HN08 cells]. 葡萄糖对小藻HN08细胞光合作用和生长的影响[j]。
Pub Date : 2017-04-04
Xiaoyu Lang, Zhiyuan Liu, Meng Xu, Lingyu Xie, Rongzhen Li

Objective: To study the potential of using glucose as carbon source to produce microalgae biomass and biochemical components, such as photosynthetic pigments, lipids, carbohydrates and proteins by tropical marine microalgae Chloralla sp. HN08.

Methods: We compared the growth characteristics of Chloralla sp. HN08 cells under photoautotrophic and mixotrophic (10 g/L glucose was added into the medium) conditions. The photosynthesis, specific growth rates, cell densities, and the content of cell's major components including lipids, starch, soluble sugar, and soluble protein were determined and compared.

Results: Glucose (10 g/L in medium) could promote Chlorella growth and increase the final cell density under light condition. However, cells declined gradually under heterotrophic condition. Under mixotrophic condition, the specific growth rate and the final cell density were 6.8 and 1.3 times as that of cells under photoautotrophic condition, respectively. The content of soluble sugar, starch, and lipids in mixotrophic cells was also significantly higher (P<0.05) than that in photoautotrophic cells. However, the content of soluble protein and photosynthetic pigments of mixotrophic cells was significantly lower (P<0.05) than that of autotrophic cells. Algae culture with glucose addition showed a higher light saturation as well as respiration rate. No significant difference in net photosynthesis rate was found between autotrophic and mixotrophic cultures (P>0.05).

Conclusion: Under light condition, glucose as a carbon source can promote lipids and starch accumulation, as well as biomass production.

目的:研究热带海洋微藻小藻(Chloralla sp. HN08)以葡萄糖为碳源生产微藻生物量及光合色素、脂类、碳水化合物和蛋白质等生化成分的潜力。方法:比较光自养和混合营养(培养基中添加10 g/L葡萄糖)条件下小藻HN08细胞的生长特性。测定并比较了光合作用、特定生长率、细胞密度以及细胞主要成分脂质、淀粉、可溶性糖和可溶性蛋白的含量。结果:在光照条件下,葡萄糖(10 g/L)能促进小球藻生长,提高最终细胞密度。而在异养条件下,细胞逐渐衰退。混合营养条件下的特定生长率和最终细胞密度分别是光自养条件下的6.8倍和1.3倍。混合营养细胞中可溶性糖、淀粉和脂质含量也显著升高(P0.05)。结论:在光照条件下,葡萄糖作为碳源可以促进脂质和淀粉的积累,促进生物量的产生。
{"title":"[Effects of glucose on photosynthesis and growth of Chloralla sp. HN08 cells].","authors":"Xiaoyu Lang,&nbsp;Zhiyuan Liu,&nbsp;Meng Xu,&nbsp;Lingyu Xie,&nbsp;Rongzhen Li","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>To study the potential of using glucose as carbon source to produce microalgae biomass and biochemical components, such as photosynthetic pigments, lipids, carbohydrates and proteins by tropical marine microalgae Chloralla sp. HN08.</p><p><strong>Methods: </strong>We compared the growth characteristics of Chloralla sp. HN08 cells under photoautotrophic and mixotrophic (10 g/L glucose was added into the medium) conditions. The photosynthesis, specific growth rates, cell densities, and the content of cell's major components including lipids, starch, soluble sugar, and soluble protein were determined and compared.</p><p><strong>Results: </strong>Glucose (10 g/L in medium) could promote Chlorella growth and increase the final cell density under light condition. However, cells declined gradually under heterotrophic condition. Under mixotrophic condition, the specific growth rate and the final cell density were 6.8 and 1.3 times as that of cells under photoautotrophic condition, respectively. The content of soluble sugar, starch, and lipids in mixotrophic cells was also significantly higher (P<0.05) than that in photoautotrophic cells. However, the content of soluble protein and photosynthetic pigments of mixotrophic cells was significantly lower (P<0.05) than that of autotrophic cells. Algae culture with glucose addition showed a higher light saturation as well as respiration rate. No significant difference in net photosynthesis rate was found between autotrophic and mixotrophic cultures (P>0.05).</p><p><strong>Conclusion: </strong>Under light condition, glucose as a carbon source can promote lipids and starch accumulation, as well as biomass production.</p>","PeriodicalId":7120,"journal":{"name":"微生物学报","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36095596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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微生物学报
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