Dominik Oleś, A. Szczepankiewicz, M. Wołuń-Cholewa, W. Butowska, P. Sobkowiak, Z. Kycler, Warchoł Jb, A. Bręborowicz
Introduction: Bronchial epithelial cell lines enable analysis of influence of different factors, such as exposure to inhaled antiasthmatic agents, on their morphology and function in vitro. Aim: To investigate whether chronic exposure to different concentrations of therapeutic agents used in the inhala - tion therapy of asthma influence bronchial epithelial cells growth and morphology. Material and methods: Bronchial epithelial cell line 16HBE14o- was used. We analyzed changes in viability between cells treated with different drug concentrations: budesonide, fluticasone propionate, salbutamol and ipratropium bromide. Observations were performed every 24 h for 4 days. Cells viability was analyzed by fluorescent staining and XTT assay. Results: An inverse correlation between drug concentration and cells viability was observed. The only exception was ipratropium bromide which was toxic at all studied concentrations. Steroids at the two highest concentrations led to a significant decrease in cells viability with fluticasone propionate being more potent than budesonide. Incuba - tion with salbutamol also demonstrated decreased cells viability at the two highest concentrations. Ipratropium bromide was toxic for the bronchial cells at all concentrations leading to a significant decrease in cells viability. Conclusions: Chronic exposure to the highest concentrations of steroids or β2-agonists decreases viability of epithe - lial cells, whereas ipratropium bromide has the strongest influence on cell viability regardless of its concentration.
{"title":"Original paper Does concentration influence viability of the bronchial epithelial cell line chronically exposed to antiasthmatic drugs","authors":"Dominik Oleś, A. Szczepankiewicz, M. Wołuń-Cholewa, W. Butowska, P. Sobkowiak, Z. Kycler, Warchoł Jb, A. Bręborowicz","doi":"10.5114/PDIA.2012.31489","DOIUrl":"https://doi.org/10.5114/PDIA.2012.31489","url":null,"abstract":"Introduction: Bronchial epithelial cell lines enable analysis of influence of different factors, such as exposure to inhaled antiasthmatic agents, on their morphology and function in vitro. Aim: To investigate whether chronic exposure to different concentrations of therapeutic agents used in the inhala - tion therapy of asthma influence bronchial epithelial cells growth and morphology. Material and methods: Bronchial epithelial cell line 16HBE14o- was used. We analyzed changes in viability between cells treated with different drug concentrations: budesonide, fluticasone propionate, salbutamol and ipratropium bromide. Observations were performed every 24 h for 4 days. Cells viability was analyzed by fluorescent staining and XTT assay. Results: An inverse correlation between drug concentration and cells viability was observed. The only exception was ipratropium bromide which was toxic at all studied concentrations. Steroids at the two highest concentrations led to a significant decrease in cells viability with fluticasone propionate being more potent than budesonide. Incuba - tion with salbutamol also demonstrated decreased cells viability at the two highest concentrations. Ipratropium bromide was toxic for the bronchial cells at all concentrations leading to a significant decrease in cells viability. Conclusions: Chronic exposure to the highest concentrations of steroids or β2-agonists decreases viability of epithe - lial cells, whereas ipratropium bromide has the strongest influence on cell viability regardless of its concentration.","PeriodicalId":7212,"journal":{"name":"Advances in Dermatology and Allergology/Postȩpy Dermatologii i Alergologii","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79071971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The authors present physical properties of laser light, operating principles, kinds of lasers and also physical and biochemical bases of biological laser light action on human cells, tissues and living organisms. The authors discuss a broad range of biological mechanisms of laserotherapy, which were examined on different levels (cell, tissue, organ) and which let them explain the positive influence of laser light in different diseases. Currently known ther apeutic effects of lasers may be in many cases beneficial, allowing for effective rehabilitation of the chronically ill, the possibility of replacing hospital-based surgery with outpatient conservative treatment (which is significantly shorter and the costs are reduced), as well as creating opportunities for the treatment of diseases as yet incurable.
{"title":"Review paper Physical and biological bases of laser phototherapy","authors":"J. Rykala, P. Szychta, H. Witmanowski","doi":"10.5114/PDIA.2012.31491","DOIUrl":"https://doi.org/10.5114/PDIA.2012.31491","url":null,"abstract":"The authors present physical properties of laser light, operating principles, kinds of lasers and also physical and biochemical bases of biological laser light action on human cells, tissues and living organisms. The authors discuss a broad range of biological mechanisms of laserotherapy, which were examined on different levels (cell, tissue, organ) and which let them explain the positive influence of laser light in different diseases. Currently known ther apeutic effects of lasers may be in many cases beneficial, allowing for effective rehabilitation of the chronically ill, the possibility of replacing hospital-based surgery with outpatient conservative treatment (which is significantly shorter and the costs are reduced), as well as creating opportunities for the treatment of diseases as yet incurable.","PeriodicalId":7212,"journal":{"name":"Advances in Dermatology and Allergology/Postȩpy Dermatologii i Alergologii","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88637227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
D. Wasiluk, E. Stefanska, L. Ostrowska, A. Serwin, A. Klepacki, B. Chodynicka
Introduction: Psoriasis is a chronic inflammatory systemic disorder. It has been suggested that dietary factors may influence the course and severity of the disease. Aim: To assess nutritive values of daily food rations (DFRs) of patients with psoriasis vulgaris and to correlate them with the severity of the disease, as well as to compare them with DFRs of patients with other chronic inflammato ry skin disorders. Material and methods: The study was conducted among 39 out-patients with psoriasis vulgaris and 18 patients with other chronic inflammatory skin disorders. The severity of psoriasis was assessed using the Psoriasis Area and Severity Index (PASI). The dietary habits were evaluated using a 24-hour interview. The energetic and nutritive value of DFRs were calculated using Dieta 2 software and the statistical analysis was performed using Statistica 9.0 software. Results: Daily food rations in psoriasis patients were characterized by an excessive intake of fats and energy derived from fats in both groups. Males and females with psoriasis consumed more monounsaturated fatty acids than con trols. The PASI value in females correlated with the total intake of fatty acids and that of monounsaturated fatty acids. The intake of vitamin D 3in females of both groups was very low. Conclusions: The nutritive value of DFRs both in psoriasis patients and in controls was imbalanced taking into con sideration various nutrients.
{"title":"Original paper Nutritive value of daily food rations of patients with psoriasis vulgaris: a preliminary report","authors":"D. Wasiluk, E. Stefanska, L. Ostrowska, A. Serwin, A. Klepacki, B. Chodynicka","doi":"10.5114/PDIA.2012.31487","DOIUrl":"https://doi.org/10.5114/PDIA.2012.31487","url":null,"abstract":"Introduction: Psoriasis is a chronic inflammatory systemic disorder. It has been suggested that dietary factors may influence the course and severity of the disease. Aim: To assess nutritive values of daily food rations (DFRs) of patients with psoriasis vulgaris and to correlate them with the severity of the disease, as well as to compare them with DFRs of patients with other chronic inflammato ry skin disorders. Material and methods: The study was conducted among 39 out-patients with psoriasis vulgaris and 18 patients with other chronic inflammatory skin disorders. The severity of psoriasis was assessed using the Psoriasis Area and Severity Index (PASI). The dietary habits were evaluated using a 24-hour interview. The energetic and nutritive value of DFRs were calculated using Dieta 2 software and the statistical analysis was performed using Statistica 9.0 software. Results: Daily food rations in psoriasis patients were characterized by an excessive intake of fats and energy derived from fats in both groups. Males and females with psoriasis consumed more monounsaturated fatty acids than con trols. The PASI value in females correlated with the total intake of fatty acids and that of monounsaturated fatty acids. The intake of vitamin D 3in females of both groups was very low. Conclusions: The nutritive value of DFRs both in psoriasis patients and in controls was imbalanced taking into con sideration various nutrients.","PeriodicalId":7212,"journal":{"name":"Advances in Dermatology and Allergology/Postȩpy Dermatologii i Alergologii","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85232810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Introduction: Recruitment of the inflammatory cells from blood to the airways in asthma is mediated by adhesive molecules, e.g. selectins and integrins. The most important integrins in cells trafficking are molecules containing α4 and β2 subunits. We hypothesized that also α1β1 and α2β1 integrins (both found by us on blood eosinophils of asthmatic subjects) are important in asthma pathogenesis. Aim: To assess the expression of selected very late antigen (VLA) subunits (α1, α2, α4 and β1) on blood CD4 and CD8 T lymphocytes from stable atopic asthmatic patients. Material and methods: The study was conducted on 25 adult atopic asthmatics (mild to moderate persistent asthma in a stable clinical condition) and 17 matched healthy controls using flow cytometry. Results: Expression of α4 and β1 on CD4 T cells was significantly higher in asthma than in controls. The α1 subunit was absent from blood lymphocytes. The α2 chain hardly detected on lymphocytes from healthy subjects was distinctively present in asthmatics. Surprisingly, in subjects suffering from asthma for longer than 4 years (n = 15), the overexpression of α2, α4 and β1 was observed on both: CD4 and CD8 T cells. Conclusions: Expression of selected VLA subunits on blood T cells may depend on asthma duration. The biological role of α2β1 integrin in asthma is unknown, but as it was described as a stimulator of collagen accumulation in the airways, α2β1 integrin could be, at least in part, responsible for asthma airway remodelling.
{"title":"Original paper Increased expression of selected very late antigen integrin subunits on CD4 and CD8 blood T lymphocytes in patients with clinically stable asymptomatic atopic asthma","authors":"S. Bazan-Socha, J. Żuk, J. Musial","doi":"10.5114/PDIA.2012.31485","DOIUrl":"https://doi.org/10.5114/PDIA.2012.31485","url":null,"abstract":"Introduction: Recruitment of the inflammatory cells from blood to the airways in asthma is mediated by adhesive molecules, e.g. selectins and integrins. The most important integrins in cells trafficking are molecules containing α4 and β2 subunits. We hypothesized that also α1β1 and α2β1 integrins (both found by us on blood eosinophils of asthmatic subjects) are important in asthma pathogenesis. Aim: To assess the expression of selected very late antigen (VLA) subunits (α1, α2, α4 and β1) on blood CD4 and CD8 T lymphocytes from stable atopic asthmatic patients. Material and methods: The study was conducted on 25 adult atopic asthmatics (mild to moderate persistent asthma in a stable clinical condition) and 17 matched healthy controls using flow cytometry. Results: Expression of α4 and β1 on CD4 T cells was significantly higher in asthma than in controls. The α1 subunit was absent from blood lymphocytes. The α2 chain hardly detected on lymphocytes from healthy subjects was distinctively present in asthmatics. Surprisingly, in subjects suffering from asthma for longer than 4 years (n = 15), the overexpression of α2, α4 and β1 was observed on both: CD4 and CD8 T cells. Conclusions: Expression of selected VLA subunits on blood T cells may depend on asthma duration. The biological role of α2β1 integrin in asthma is unknown, but as it was described as a stimulator of collagen accumulation in the airways, α2β1 integrin could be, at least in part, responsible for asthma airway remodelling.","PeriodicalId":7212,"journal":{"name":"Advances in Dermatology and Allergology/Postȩpy Dermatologii i Alergologii","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78924700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Polańska, A. Dańczak‑Pazdrowska, W. Silny, D. Jenerowicz, A. Osmola-Mańkowska, K. Olek-Hrab
Introduction: Atopic dermatitis (AD) is an inflammatory pruritic dermatosis, which is characterized by an impaired skin barrier function manifested as an increased transepidermal water loss (TEWL). Aim: Presentation results of instrumental evaluation of the skin barrier function in AD patients in relation to the dis ease severity as well as pruritus. Material and methods: Fifty-five AD patients aged from 8 to 60 years were enrolled to the study. Epidermal barrier function (TEWL measurement and corneometry) as well as erythema measurement were determined in each patient within the same affected skin region, antecubital fossa. Clinical evaluation was performed using the W-AZS scor ing system. Results: There was a statistically significant difference in the mean TEWL values between groups of patients with different degrees of skin inflammation expressed by the W-AZS index, while no significant differences were observed in relation to the skin hydration and ertyhema measurement. There was a statistically significant correlation between the W-AZS index and TEWL as well as between the W-AZS I index and TEWL. Conclusions: Selected parameters of the skin barrier function (TEWL, skin hydration) and the level of erythema are useful in evaluation of AD. Transepidermal water loss measurement presents a good correlation with W-AZS and with intensity of patients itching. Instrumental assessment provides us with a fast and objective evaluation of the eczema status, what seems to be very important in the era of evidence-based medicine.
{"title":"Original paper Evaluation of selected skin barrier functions in atopicdermatitis in relation to the disease severity and pruritus","authors":"A. Polańska, A. Dańczak‑Pazdrowska, W. Silny, D. Jenerowicz, A. Osmola-Mańkowska, K. Olek-Hrab","doi":"10.5114/PDIA.2012.31492","DOIUrl":"https://doi.org/10.5114/PDIA.2012.31492","url":null,"abstract":"Introduction: Atopic dermatitis (AD) is an inflammatory pruritic dermatosis, which is characterized by an impaired skin barrier function manifested as an increased transepidermal water loss (TEWL). Aim: Presentation results of instrumental evaluation of the skin barrier function in AD patients in relation to the dis ease severity as well as pruritus. Material and methods: Fifty-five AD patients aged from 8 to 60 years were enrolled to the study. Epidermal barrier function (TEWL measurement and corneometry) as well as erythema measurement were determined in each patient within the same affected skin region, antecubital fossa. Clinical evaluation was performed using the W-AZS scor ing system. Results: There was a statistically significant difference in the mean TEWL values between groups of patients with different degrees of skin inflammation expressed by the W-AZS index, while no significant differences were observed in relation to the skin hydration and ertyhema measurement. There was a statistically significant correlation between the W-AZS index and TEWL as well as between the W-AZS I index and TEWL. Conclusions: Selected parameters of the skin barrier function (TEWL, skin hydration) and the level of erythema are useful in evaluation of AD. Transepidermal water loss measurement presents a good correlation with W-AZS and with intensity of patients itching. Instrumental assessment provides us with a fast and objective evaluation of the eczema status, what seems to be very important in the era of evidence-based medicine.","PeriodicalId":7212,"journal":{"name":"Advances in Dermatology and Allergology/Postȩpy Dermatologii i Alergologii","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80992390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Kuźmiński, M. Przybyszewski, M. Graczyk, Z. Bartuzi
During the migration to the site of inflammation, allergic inflammatory cells must overcome several structures that constitute an impregnable barrier under normal conditions. One such structure is the extracellular matrix respon sible for closely filling the intercellular space of tissues. The loosening of this tight structure by the activity of spe cific tissue enzymes, i.e. extracellular matrix metalloproteinases (MMPs), enables the influx of effector cells to the site of inflammation, thus facilitating the development of allergic inflammation and sustaining its presence. Specific tissue inhibitors of metalloproteinases (TIMPs) represent the most important factor involved in the regu lation of MMP activity. Under physiological conditions, MMPs and TIMPs co-exist in a particular specific equilibri um, which is disturbed under pathological circumstances. The MMP-2 and MMP-9, referred to as gelatinases, belong to the best-studied MMPs associated with the development of allergic disorders. Their role pertains to the degra dation of type IV collagen, the main component of basal membranes; this facilitates the influx of cells to the site of allergic inflammation.
{"title":"Review paper The role of extracellular matrix metalloproteinases and their inhibitors in allergic diseases","authors":"A. Kuźmiński, M. Przybyszewski, M. Graczyk, Z. Bartuzi","doi":"10.5114/PDIA.2012.31493","DOIUrl":"https://doi.org/10.5114/PDIA.2012.31493","url":null,"abstract":"During the migration to the site of inflammation, allergic inflammatory cells must overcome several structures that constitute an impregnable barrier under normal conditions. One such structure is the extracellular matrix respon sible for closely filling the intercellular space of tissues. The loosening of this tight structure by the activity of spe cific tissue enzymes, i.e. extracellular matrix metalloproteinases (MMPs), enables the influx of effector cells to the site of inflammation, thus facilitating the development of allergic inflammation and sustaining its presence. Specific tissue inhibitors of metalloproteinases (TIMPs) represent the most important factor involved in the regu lation of MMP activity. Under physiological conditions, MMPs and TIMPs co-exist in a particular specific equilibri um, which is disturbed under pathological circumstances. The MMP-2 and MMP-9, referred to as gelatinases, belong to the best-studied MMPs associated with the development of allergic disorders. Their role pertains to the degra dation of type IV collagen, the main component of basal membranes; this facilitates the influx of cells to the site of allergic inflammation.","PeriodicalId":7212,"journal":{"name":"Advances in Dermatology and Allergology/Postȩpy Dermatologii i Alergologii","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90474601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
P. Chomik, Adam Michcik, I. Michajłowski, M. Sobjanek, A. Włodarkiewicz
The investigators wish to discuss the diagnostic difficulties, histology and immunohistochemical profile of a soli tary fibrous tumor (SFT) based on the presented case, as well as previously reported cases within the oral cavity. A young woman was referred to the Department of Maxillofacial Surgery, Medical University of Gdansk, Poland, due to a considerable palpable mass within the hard palate. Pain and discomfort caused by the tumor’s location were the main complaints. A clinical examination revealed a tumor of the right hard palate along the alveolar crest, measuring approximately 5 cm ◊ 3 cm. Panoramic X-ray depicted a bone defect of the alveolar crest around tooth 17. Histological and immunohistochemical evaluation of the biopsy specimen and resected tumor established the diagnosis of SFT. This rare spindle cell neoplasm of mesenchymal origin is typical of serosal sites and approxi mately 80 cases within the head and neck region have been reported so far. The hard palate is one of the least fre quent locations. Approximately 5-20% of lesions may present features of aggressiveness. The authors wish to empha size that the diagnosis of SFT is challenging due to a relatively small number of cases reported in the literature, uncertain etiology of the tumor, as well as clinical and histological similarity to other, more frequently occurring benign neoplasms of mesenchymal origin, i.e. hemangiopericytoma, myofibroblastoma, schwannoma, neurofibro ma, leiomyoma, as well as inflammatory disorders, especially nodular fasciitis. Radical excision of the tumor is mandatory for effective treatment due to the possibility of recurrence and malignant transformation after subrad ical resection.
{"title":"Case report Solitary fibrous tumor in the oral cavity: a case report and diagnostic dilemma","authors":"P. Chomik, Adam Michcik, I. Michajłowski, M. Sobjanek, A. Włodarkiewicz","doi":"10.5114/PDIA.2012.31495","DOIUrl":"https://doi.org/10.5114/PDIA.2012.31495","url":null,"abstract":"The investigators wish to discuss the diagnostic difficulties, histology and immunohistochemical profile of a soli tary fibrous tumor (SFT) based on the presented case, as well as previously reported cases within the oral cavity. A young woman was referred to the Department of Maxillofacial Surgery, Medical University of Gdansk, Poland, due to a considerable palpable mass within the hard palate. Pain and discomfort caused by the tumor’s location were the main complaints. A clinical examination revealed a tumor of the right hard palate along the alveolar crest, measuring approximately 5 cm ◊ 3 cm. Panoramic X-ray depicted a bone defect of the alveolar crest around tooth 17. Histological and immunohistochemical evaluation of the biopsy specimen and resected tumor established the diagnosis of SFT. This rare spindle cell neoplasm of mesenchymal origin is typical of serosal sites and approxi mately 80 cases within the head and neck region have been reported so far. The hard palate is one of the least fre quent locations. Approximately 5-20% of lesions may present features of aggressiveness. The authors wish to empha size that the diagnosis of SFT is challenging due to a relatively small number of cases reported in the literature, uncertain etiology of the tumor, as well as clinical and histological similarity to other, more frequently occurring benign neoplasms of mesenchymal origin, i.e. hemangiopericytoma, myofibroblastoma, schwannoma, neurofibro ma, leiomyoma, as well as inflammatory disorders, especially nodular fasciitis. Radical excision of the tumor is mandatory for effective treatment due to the possibility of recurrence and malignant transformation after subrad ical resection.","PeriodicalId":7212,"journal":{"name":"Advances in Dermatology and Allergology/Postȩpy Dermatologii i Alergologii","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73433931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Introduction: A bee pollen allergy is rare and often confused with a pollen allergy. The possibility of inducing an allergy in pollen allergic patients by bee pollen is still controversial. Aim: To determine the prevalence of bee pollen allergy in beekeepers and their families and its possible associa tion with other conditions. Material and methods: The questionnaire study was carried out on 493 Polish beekeepers. The questionnaire was based on the current literature and touched on several aspects of a bee products allergy. Results: Only 19.4% (493 from 2540 questionnaires sent) of questionnaires were returned by 15 October 2011. From the total group, only 2 beekeepers reported adverse reactions after bee pollen ingestion. They presented only minor reactions. From 493 beekeepers, 43 responders reported other allergies. From that group, no one reported a con comitant bee pollen allergy. Additionally, beekeepers reported only 22 cases of bee pollen intolerance among their customers and in family members a bee pollen allergy occurred in 0.56% of cases. Conclusions: This preliminary study provides some new aspects on the bee pollen allergy. The thesis that a bee pol len allergy is associated with the occurrence of proteins from bee saliva rather than the occurrence of anemophilous plant pollen should be evaluated in future studies.
{"title":"Original paper Bee pollen allergy in Polish beekeepers and their families","authors":"K. Basista, B. Filipek, K. Sodzawiczny","doi":"10.5114/PDIA.2012.31486","DOIUrl":"https://doi.org/10.5114/PDIA.2012.31486","url":null,"abstract":"Introduction: A bee pollen allergy is rare and often confused with a pollen allergy. The possibility of inducing an allergy in pollen allergic patients by bee pollen is still controversial. Aim: To determine the prevalence of bee pollen allergy in beekeepers and their families and its possible associa tion with other conditions. Material and methods: The questionnaire study was carried out on 493 Polish beekeepers. The questionnaire was based on the current literature and touched on several aspects of a bee products allergy. Results: Only 19.4% (493 from 2540 questionnaires sent) of questionnaires were returned by 15 October 2011. From the total group, only 2 beekeepers reported adverse reactions after bee pollen ingestion. They presented only minor reactions. From 493 beekeepers, 43 responders reported other allergies. From that group, no one reported a con comitant bee pollen allergy. Additionally, beekeepers reported only 22 cases of bee pollen intolerance among their customers and in family members a bee pollen allergy occurred in 0.56% of cases. Conclusions: This preliminary study provides some new aspects on the bee pollen allergy. The thesis that a bee pol len allergy is associated with the occurrence of proteins from bee saliva rather than the occurrence of anemophilous plant pollen should be evaluated in future studies.","PeriodicalId":7212,"journal":{"name":"Advances in Dermatology and Allergology/Postȩpy Dermatologii i Alergologii","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76264678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Introduction: Phenolic compounds have inhibitory effects on tyrosinase and inflammatory activities which lead to reduction in the melanin and erythema content. This is important for hyperpigmentation, dermatitis and other oxidative stress diseases. Aim: Investigation of the effects of a cream containing phenolic-rich extract of Acacia bark on the human skin melanin and erythema content. Material and methods: Eleven healthy male volunteers were analyzed and treated with active cream versus base for a period of three months by non-invasive bioengineering techniques. Results: No serious adverse effects were observed after patch testing. Base showed insignificant results and active cream showed a significant decrease in skin melanin and erythema content after statistically applied ANOVA. Conclusions: Our results show that the Acacia bark extract represents a valuable source of phenolic compounds produced naturally. Treatments of human skin with active cream containing phenolic-rich Acacia bark extract sig nificantly reduce skin melanin and erythema in vivo and could be used for topical treatment of hyperpigmentation and dermatitis.
{"title":"Original paper In vivo evaluation: the effects of a cream containing Acacia bark extract on skin melanin and erythema content","authors":"Atif Ali, N. Akhtar, Muhammad Shoaib Khan","doi":"10.5114/PDIA.2012.31490","DOIUrl":"https://doi.org/10.5114/PDIA.2012.31490","url":null,"abstract":"Introduction: Phenolic compounds have inhibitory effects on tyrosinase and inflammatory activities which lead to reduction in the melanin and erythema content. This is important for hyperpigmentation, dermatitis and other oxidative stress diseases. Aim: Investigation of the effects of a cream containing phenolic-rich extract of Acacia bark on the human skin melanin and erythema content. Material and methods: Eleven healthy male volunteers were analyzed and treated with active cream versus base for a period of three months by non-invasive bioengineering techniques. Results: No serious adverse effects were observed after patch testing. Base showed insignificant results and active cream showed a significant decrease in skin melanin and erythema content after statistically applied ANOVA. Conclusions: Our results show that the Acacia bark extract represents a valuable source of phenolic compounds produced naturally. Treatments of human skin with active cream containing phenolic-rich Acacia bark extract sig nificantly reduce skin melanin and erythema in vivo and could be used for topical treatment of hyperpigmentation and dermatitis.","PeriodicalId":7212,"journal":{"name":"Advances in Dermatology and Allergology/Postȩpy Dermatologii i Alergologii","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81207010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Anna Szponar-Bojda, D. Krasowska, A. Pietrzak, G. Chodorowska
Introduction: Recent years’ studies have revealed a link between the metabolic syndrome and psoriasis. The com mon elements of pathogenesis of both diseases, primarily associated with severe inflammation, may be responsi ble for an increased incidence of metabolic syndrome components in psoriasis patients. Aim: To evaluate the prevalence of selected risk factors for the metabolic syndrome in patients with psoriasis and possible involvement of these factors in the severity of psoriasis. Material and methods: The study included 117 adult psoriatic patients compared with a control group. The severi ty of psoriasis was evaluated using the PASI and mNAPSI score. Basic laboratory tests were performed. The body mass index (BMI), De Ritis ratio and blood pressure were determined for each patient. Results: In the psoriasis group, excess body weight and abnormal values of BMI were significantly more frequent. Body mass index and body weight were significantly higher in patients with the calculated De Ritis ratio than in other subjects. The levels of erythrocyte sedimentation rate and C-reactive protein were significantly higher in the psoriasis group and positively correlated with BMI. Compared with controls, patients with psoriasis had significantly higher values of systolic and diastolic blood pressure, with no correlation with the severity of psoriasis evaluated using the PASI and mNAPSI score. Conclusions: The results obtained in our study indicate that psoriatic patients appear to be at a higher risk of the metabolic disorders such as hypertension, obesity, liver dysfunction, increased inflammatory parameters than healthy subjects, irrespective of the full manifestation of the metabolic syndrome. We suggest that patients with psoriasis should be routinely screened for the presence of the above-mentioned disorders.
{"title":"Original paper Metabolic syndrome in psoriasis","authors":"Anna Szponar-Bojda, D. Krasowska, A. Pietrzak, G. Chodorowska","doi":"10.5114/PDIA.2012.31488","DOIUrl":"https://doi.org/10.5114/PDIA.2012.31488","url":null,"abstract":"Introduction: Recent years’ studies have revealed a link between the metabolic syndrome and psoriasis. The com mon elements of pathogenesis of both diseases, primarily associated with severe inflammation, may be responsi ble for an increased incidence of metabolic syndrome components in psoriasis patients. Aim: To evaluate the prevalence of selected risk factors for the metabolic syndrome in patients with psoriasis and possible involvement of these factors in the severity of psoriasis. Material and methods: The study included 117 adult psoriatic patients compared with a control group. The severi ty of psoriasis was evaluated using the PASI and mNAPSI score. Basic laboratory tests were performed. The body mass index (BMI), De Ritis ratio and blood pressure were determined for each patient. Results: In the psoriasis group, excess body weight and abnormal values of BMI were significantly more frequent. Body mass index and body weight were significantly higher in patients with the calculated De Ritis ratio than in other subjects. The levels of erythrocyte sedimentation rate and C-reactive protein were significantly higher in the psoriasis group and positively correlated with BMI. Compared with controls, patients with psoriasis had significantly higher values of systolic and diastolic blood pressure, with no correlation with the severity of psoriasis evaluated using the PASI and mNAPSI score. Conclusions: The results obtained in our study indicate that psoriatic patients appear to be at a higher risk of the metabolic disorders such as hypertension, obesity, liver dysfunction, increased inflammatory parameters than healthy subjects, irrespective of the full manifestation of the metabolic syndrome. We suggest that patients with psoriasis should be routinely screened for the presence of the above-mentioned disorders.","PeriodicalId":7212,"journal":{"name":"Advances in Dermatology and Allergology/Postȩpy Dermatologii i Alergologii","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75844986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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