Biomaterials and biosystems最新文献_第2页

Gallic acid released by a layered double hydroxide-coated scaffold of hydroxyapatite and β-tricalcium phosphate inhibits the osteoclast formation In Vitro 羟基磷灰石和β-磷酸三钙层状双羟基包被支架释放没食子酸抑制体外破骨细胞的形成

Q3 Biochemistry, Genetics and Molecular Biology

Biomaterials and biosystems

Pub Date : 2025-08-20 DOI: 10.1016/j.bbiosy.2025.100119

Chiara Suvieri , Maria Bastianini , Stefano Pagano , Lorella Marinucci , Valeria Ambrogi , Leonardo Leonardi , Carmela Conte , Maria Teresa Pallotta , Bernard Fioretti , Giovanna Traina , Michele Sisani , Maria Laura Belladonna

Following dental extraction, alveolar bone loss, driven by the osteoclast (OC) bone-eroding cells, is a relevant concern in dental practice since it could compromise the possibility of installing dental implants. This study aimed to develop a drug delivery system releasing the antiosteoclastogenic molecule gallic acid (GA) at the alveolar bone level to control the dysregulated balance between OCs and bone-building osteoblasts and thus delay bone erosion. We functionalized small blocks of the hydroxyapatite- and β-tricalcium phosphate-based RIGENERA BTK BCP biomaterial with layered double hydroxide (LDH) and GA (RIG_LDH-GA). By the in vitro model of Receptor Activator of Nuclear factor Kappa-Β Ligand (RANKL)-induced osteoclastogenesis in RAW 264.7 macrophages, we demonstrated that the conditioned medium (CM) obtained after 1-day incubation with RIG_LDH-GA contrasts the OC formation in a dose-dependent manner until a complete inhibition at the highest tested dose, while the unfunctionalized control (RIG) is ineffective. TRAP enzyme activity, OC marker gene expression, and bone resorption activity confirmed the antiosteoclastogenic effect of RIG_LDH-GA CM. Moreover, the expression of RANK (the RANKL’s receptor), otherwise induced by RANKL treatment, was reduced to the untreated control extent, consistent with the decreased expression of the transcription factors c-Fos and NFATc1, activated downstream in the RANK signaling pathway and inducing RANK itself. Thus, since GA released by the RIG_LDH-GA system effectively exerted an antiosteoclastogenic effect, RIGENERA BTK BCP functionalization with LDH and GA likely appears to be an osteoprotective upgrade of this biomaterial, already possessing bone regenerative properties, and might find successful clinical application in preventing osteoclast-mediated alveolar bone loss.

拔牙后，破骨细胞（OC）骨侵蚀细胞导致的牙槽骨丢失是牙科实践中一个值得关注的问题，因为它可能会影响种植体的安装。本研究旨在开发一种在牙槽骨水平释放抗破骨细胞分子没食子酸（GA）的药物递送系统，以控制OCs与成骨细胞之间的失调平衡，从而延缓骨侵蚀。我们用层状双氢氧化物（LDH）和GA （RIG_LDH-GA）功能化了小块羟基磷灰石和β-磷酸三钙基的RIGENERA BTK BCP生物材料。通过RANKL诱导RAW 264.7巨噬细胞破骨形成的体外模型，我们证明了与RIG_LDH-GA孵育1天后获得的条件培养基（CM）以剂量依赖的方式对比OC的形成，直到在最高测试剂量下完全抑制，而非功能化对照（RIG）无效。TRAP酶活性、OC标记基因表达和骨吸收活性证实了RIG_LDH-GA CM的抗破骨作用。此外，RANKL处理诱导的RANKL受体RANK的表达降低到未处理的对照水平，这与在RANK信号通路下游激活并诱导RANK本身的转录因子c-Fos和NFATc1表达降低一致。因此，由于RIG_LDH-GA系统释放的GA有效地发挥了抗破骨作用，因此LDH和GA对RIGENERA BTK BCP的功能化可能是对这种已经具有骨再生特性的生物材料的骨保护升级，并可能在预防破骨细胞介导的牙槽骨丢失方面获得成功的临床应用。

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引用次数: 0

Integrated approach to cell growth and recovery in silk fibroin scaffolds via a spin-down system 丝素蛋白支架中细胞生长和恢复的综合方法

Q3 Biochemistry, Genetics and Molecular Biology

Biomaterials and biosystems

Pub Date : 2025-08-11 DOI: 10.1016/j.bbiosy.2025.100118

Irem Duman , Verena Schwingenschlögl-Maisetschläger , Ceren Okuducu , Christian Kraule , Haider Sami , Manfred Ogris , Andreas Teuschl-Woller , Verena Pichler

Silk fibroin scaffolds are a versatile platform for biomedical applications due to their biocompatibility and tunable properties. Successful clinical translation requires standardized production and characterization methods to ensure high reproducibility in cell seeding, growth profiling and recovery for downstream analysis. The intrinsic autofluorescence of silk and the limited diffusion of reagents through its porous structure present significant challenges for conventional assays, such as cell viability tests, DNA quantification, and optical imaging-based approaches. These assays are a requirement for validation procedures and characterization. In this study, we introduce a standardized protocol for efficiently assessing cell seeding and growth behavior. By analyzing the physicochemical properties of the silk sponge, we determined the optimal volumes required for silk swelling and cell seeding. Additionally, we developed a spin-down system that enables the application of endpoint assays while ensuring gentle cell recovery. We established and experimentally validated the relationship between silk sponge volume and the growth limitations of embedded cells. Overall, this study underscores the importance of a standardized procedure for efficient cell seeding and recovery, ultimately facilitating clinical translation.

丝素蛋白支架由于其生物相容性和可调特性而成为生物医学应用的通用平台。成功的临床翻译需要标准化的生产和表征方法，以确保细胞播种、生长分析和下游分析的恢复的高重复性。丝绸固有的自身荧光和试剂通过其多孔结构的有限扩散对传统的分析提出了重大挑战，如细胞活力测试、DNA定量和基于光学成像的方法。这些试验是验证程序和表征的要求。在这项研究中，我们引入了一个标准化的方案来有效地评估细胞播种和生长行为。通过分析海绵蚕丝的理化性质，确定了海绵蚕丝膨胀和细胞播种所需的最佳体积。此外，我们还开发了一种spin-down系统，可以在确保细胞恢复的同时应用端点分析。我们建立并实验验证了海绵体积与包埋细胞生长限制之间的关系。总的来说，这项研究强调了有效的细胞播种和恢复的标准化程序的重要性，最终促进了临床转化。

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引用次数: 0

The emerging role of biomaterial applications in cerebral lymphatic surgical interventions: A narrative review 生物材料在脑淋巴手术干预中的应用：综述

Q3 Biochemistry, Genetics and Molecular Biology

Biomaterials and biosystems

Pub Date : 2025-08-09 DOI: 10.1016/j.bbiosy.2025.100117

Mark Jessup , Nicholas J. Iglesias , Kashyap K. Tadisina , Kyle Xu , Juan Mella-Catinchi , Leonard Pinchuk , Devinder Singh , David T. Tse , Vasudev Vivekanand Nayak , Paulo G. Coelho

The cerebral lymphatic system plays a critical role in central nervous system (CNS) homeostasis through fluid regulation, toxin clearance, and immune modulation. Recent discoveries in the glial-lymphatic (glymphatic) and meningeal lymphatic systems have demonstrated their involvement in a spectrum of CNS pathologies such as Alzheimer's disease (AD), Parkinson’s disease (PD), Huntington's disease (HD), multiple sclerosis (MS), traumatic brain injury (TBI), stroke, and cancers. This review summarizes current understanding of the cerebral lymphatic system’s mechanisms and their contribution to CNS health. Furthermore, we emphasize the linkage of lymphatic dysfunction and the pathogenesis of neurodegenerative and neuroinflammatory etiologies. Lymphatic alterations such as deep cervical lymph node (DCLN) manipulation have been shown to increase function, reduce toxin accumulation and improve disease. Other techniques like supermicrosurgical interventions of lymphaticovenular (lymphovenous) anastomosis and lymphatic reconstruction have recently demonstrated therapeutic benefits. Novel approaches of supermicrosurgical techniques, such as cervical shunting to decompress lymphatic systems (CSULS), have displayed promising cognitive improvements in AD patients. We additionally explore the role of biomaterials in improving interventional outcomes and their potential to be applied to lymphatic surgical developments. Despite their widespread applications in surgical practice, the use of biomaterials in cerebral lymphatic reconstruction remains unexplored. This review discusses the potential benefits of integrating biomaterials into emerging lymphatic interventions to improve patient outcomes.

脑淋巴系统通过体液调节、毒素清除和免疫调节在中枢神经系统（CNS）稳态中起着关键作用。最近在神经胶质淋巴系统（glymatic）和脑膜淋巴系统的发现表明，它们参与了一系列中枢神经系统病理，如阿尔茨海默病（AD）、帕金森病（PD）、亨廷顿病（HD）、多发性硬化症（MS）、创伤性脑损伤（TBI）、中风和癌症。本文综述了目前对脑淋巴系统机制及其对中枢神经系统健康的贡献的理解。此外，我们强调淋巴功能障碍与神经退行性和神经炎症病因的发病机制的联系。淋巴改变，如颈深淋巴结（DCLN）操作已被证明可以增加功能，减少毒素积累和改善疾病。其他技术，如超显微手术干预淋巴-小静脉（淋巴静脉）吻合和淋巴重建最近显示出治疗效益。超显微外科技术的新方法，如颈椎分流减压淋巴系统（CSULS），已经显示出有希望改善AD患者的认知能力。我们还探讨了生物材料在改善介入结果中的作用及其在淋巴外科发展中的应用潜力。尽管生物材料在外科实践中广泛应用，但其在脑淋巴重建中的应用仍未得到探索。本综述讨论了将生物材料整合到新兴淋巴干预中以改善患者预后的潜在益处。

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引用次数: 0

Mimicking scaffold membrane based electrospun polyurethane fibers with functional extracellular-like component coating for guided bone regeneration scaffolds 带功能细胞外成分涂层的仿支架膜基静电纺聚氨酯纤维用于引导骨再生支架

Q3 Biochemistry, Genetics and Molecular Biology

Biomaterials and biosystems

Pub Date : 2025-08-08 DOI: 10.1016/j.bbiosy.2025.100116

Nattawat Watcharajittanont , Worasak Prarokijjak , Chayada Teanchai , Kanon Jatuworapruk , Jirut Meesane

Mimicking scaffold membranes for guided bone regeneration were fabricated using electrospun polyurethane (PU) coated with polyvinyl alcohol (PVA) and sodium alginate. Five different proportions of PVA and sodium alginate (AGN) were compared as coating solutions for the electrospun PU membranes. Molecular organization, morphology, and physical, mechanical, and biological properties were investigated. The results demonstrated that the electro-spun PU membranes with PVA and sodium alginate coating showed molecular organization via chemical bonding. The coated membranes, especially PU/PVA:AGN (30:70), exhibited higher hydrophilicity, maximum load, and elasticity than membranes without coating, and they exhibited better cell adhesion, cell proliferation, ALP activity, and calcium deposition. In conclusion, we determined that our PU/PVA:AGN (30:70) periosteum mimicking membrane is a promising candidate for guided bone regeneration applications.

采用静电纺聚氨酯（PU）包覆聚乙烯醇（PVA）和海藻酸钠制备了用于引导骨再生的模拟支架膜。比较了五种不同配比的聚乙烯醇（PVA）和海藻酸钠（AGN）作为静电纺PU膜的涂覆溶液。研究了分子的组织、形态、物理、机械和生物特性。结果表明，涂有PVA和海藻酸钠的静电纺PU膜通过化学键形成分子结构。包覆后的膜，特别是PU/PVA:AGN(30:70)，比未包覆的膜表现出更高的亲水性、最大负荷和弹性，并表现出更好的细胞粘附、细胞增殖、ALP活性和钙沉积。总之，我们确定我们的PU/PVA:AGN（30:70）骨膜模拟膜是引导骨再生应用的有希望的候选材料。

引用次数: 0

Gynecologic postoperative anti-adhesion barriers: From biomaterials to barrier development 妇科术后抗粘连屏障：从生物材料到屏障的发展

Q3 Biochemistry, Genetics and Molecular Biology

Biomaterials and biosystems

Pub Date : 2025-08-05 DOI: 10.1016/j.bbiosy.2025.100115

Abbas Fazel Anvari-Yazdi , Daniel J. MacPhee , Ildiko Badea , Xiongbiao Chen

Gynecologic postoperative adhesions (GPOA) remain an under-appreciated source of morbidity despite advances in minimally invasive surgery. Adhesions forming after myomectomy, extensive endometriosis excision, repeat caesarean section, or hysteroscopic adhesiolysis develop in 20 – 90 % of patients and account for up to 40 % of secondary infertility, chronic pelvic pain, bowel obstruction, and life-threatening obstetric complications such as placenta accreta spectrum. Because the uterus is hormonally responsive and destined for potential pregnancy, anti-adhesion barriers for gynecologic tissues must meet stricter criteria for biocompatibility, resorption timing, teratogenic safety, and reproductive regulatory classification than barriers designed for bowel or tendon repair.

This review consolidates the rapidly expanding literature on biomaterial-based barriers specifically tailored for gynecologic applications. We first dissect the pathophysiology of uterine and adnexal adhesion formation—including mesothelial disruption, fibrin persistence, and estrogen-modulated wound remodeling—to highlight design targets unique to the female reproductive tract. Next, we critically appraise natural and synthetic polymers, discussing how formulation parameters govern in-vivo elimination or excretion routes and influence fertility outcomes. Cutting-edge fabrication strategies—such as electrospinning, 3D bioprinting, melt electrowriting, Janus hydrogels, and microneedle patches—are reviewed with an eye toward uterine conformity, minimally invasive deployability, and on-demand release of drugs or exosomes. We further map current FDA-cleared films (INTERCEED™, Seprafilm®, SurgiWrap®) against unmet gynecologic needs, delineate limitations, and identify opportunities for multifunctional, self-healing, image-visible, and patient-specific barrier platforms.

By framing adhesion prevention through a gynecologic lens, this article provides clinicians, materials scientists, and device developers with a roadmap for translating next-generation barriers from bench to bedside, ultimately reducing infertility, surgical re-admission, and obstetric risk in women worldwide.

尽管微创手术取得了进展，妇科术后粘连（GPOA）仍然是一个未被充分认识的发病率来源。20 - 90%的患者在子宫肌瘤切除术、广泛子宫内膜异位症切除术、重复剖宫产或宫腔镜下粘连松解术后形成粘连，占继发性不孕症、慢性盆腔疼痛、肠梗阻和危及生命的产科并发症（如胎盘增生）的40%。由于子宫对激素有反应，并且注定要怀孕，因此妇科组织的抗粘连屏障必须满足比用于肠或肌腱修复的屏障更严格的生物相容性、吸收时间、致畸安全性和生殖调节分类标准。这篇综述整合了快速扩展的专门针对妇科应用的生物材料屏障的文献。我们首先剖析子宫和附件粘连形成的病理生理学，包括间皮破坏、纤维蛋白持久性和雌激素调节的伤口重塑，以突出女性生殖道独特的设计目标。接下来，我们批判性地评估天然和合成聚合物，讨论配方参数如何控制体内消除或排泄途径并影响生育结果。尖端的制造策略-如静电纺丝，3D生物打印，熔融电书写，Janus水凝胶，微针贴片-回顾着眼于子宫整合，微创部署，按需释放药物或外泌体。我们进一步将目前fda批准的膜（INTERCEED™，separfilm®，SurgiWrap®）与未满足的妇科需求进行对比，描述局限性，并确定多功能，自我修复，图像可见和患者特异性屏障平台的机会。通过从妇科角度构建粘连预防，本文为临床医生、材料科学家和设备开发人员提供了将下一代屏障从实验台转化为床边的路线图，最终减少全世界妇女的不孕症、手术再入院和产科风险。

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引用次数: 0

Substrate stiffness and pressure alter retinal Müller glia response and extracellular matrix production 基质硬度和压力改变视网膜<s:1>神经胶质反应和细胞外基质的产生

Q3 Biochemistry, Genetics and Molecular Biology

Biomaterials and biosystems

Pub Date : 2025-07-07 DOI: 10.1016/j.bbiosy.2025.100114

Laura Prieto-López , Xandra Pereiro , Emilio J. González Ramírez , Noelia Ruzafa , Alicia Alonso , Kristian Franze , Elena Vecino

Background

The retina is highly influenced by its mechanical environment, with Müller glia (MG) acting as key mechanosensors and extracellular matrix (ECM) producers. This study examined MG responses to substrate stiffness and high pressure (HP), and whether TGF-β1 modulation could mitigate these effects.

Methods

Primary MG from adult rat retinas were cultured on glass (Young’s modulus E’=∼1 gigapascal (GPa)) and polyacrylamide gels (10 kPa and 100 kPa). MG were exposed to atmospheric and 70 mmHg (HP) conditions, with TGF-β1 pharmacologically blocked.

Results

On glass and 100 kPa gels, MG survival, cell area, and ECM deposition (collagen I, IV, and fibronectin) increased, with cells adopting a fusiform shape and more dedifferentiated state. Under HP, survival decreased on stiffer substrates, though cell area and morphology remained unchanged. HP increased ECM deposition, which was reduced by TGF-β1 inhibition.

Conclusions

Our findings suggest that MG response to mechanical stress alter their survival and cell area, and increases ECM secretion, highlighting TGF-β1 as a potential therapeutic target.

视网膜受其机械环境的高度影响，突触神经胶质（MG）是关键的机械传感器和细胞外基质（ECM）的产生者。本研究检测了MG对基质刚度和高压（HP）的响应，以及TGF-β1调节是否可以减轻这些影响。方法用玻璃（杨氏模量E′= ~ 1gigapascal (GPa)）和聚丙烯酰胺凝胶（10kpa和100kpa）培养成年大鼠视网膜原代MG。MG暴露于大气和70 mmHg （HP）条件下，TGF-β1被药物阻断。结果玻璃凝胶和100 kPa凝胶的MG存活率、细胞面积和ECM沉积（胶原I、IV、纤维连接蛋白）均增加，细胞呈梭状，呈去分化状态。HP作用下，在较硬的基质上存活率下降，但细胞面积和形态保持不变。HP增加了ECM沉积，而抑制TGF-β1则降低了ECM沉积。结论MG对机械应力的反应改变了它们的存活和细胞面积，增加了ECM的分泌，提示TGF-β1是潜在的治疗靶点。

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引用次数: 0

Evaluating the potential hepatotoxicity from hip implant wear products—An in-vitro and in-vivo study 评估髋关节植入物的潜在肝毒性——一项体外和体内研究

Q3 Biochemistry, Genetics and Molecular Biology

Biomaterials and biosystems

Pub Date : 2025-05-18 DOI: 10.1016/j.bbiosy.2025.100113

Shriya Thakur , Hemalatha Kanniyappan , Puranjay Gupta , Govindaraj Perumal , Robert Hillwig , Vedant V. Bodke , Salman R. Khetani , Mathew T. Mathew

Total hip replacement (THR) is recognized as an effective treatment for patients suffering from severe arthritis or hip issues, with approximately 2.5 million hip and knee replacements recorded annually (AJJR, 2021). A significant clinical concern associated with THR is the toxicity caused by metal particles and ions released from the implant surfaces, which can damage local tissue and potentially spread to distant organs, resulting in systemic toxicity. The toxicity, influenced by the size and concentration of the particles and ions, is especially critical in the liver, the body's main metabolic organ and a primary site for implant accumulation. In this study, we aim to investigate the hepatotoxicity of increasing concentrations of cobalt-chromium-molybdenum (CoCrMo) and titanium (Ti) particles/ions (generally called wear products) in remote organs, particularly the liver. We found that these particles and ions enter cells through metal ion transporters and phagocytosis, leading to significant cellular damage, with titanium ions exhibiting the highest toxicity levels, followed by cobalt ions and CoCrMo particles. Our combined in-vitro and in-vivo research supports the hypothesis that metal particles and ions from implants pose a substantial risk of liver cell damage. This underscores the importance of addressing the systemic impacts of implant-derived metal toxicity in patients with THR.

全髋关节置换术（THR）被认为是治疗严重关节炎或髋关节问题的有效方法，每年约有250万例髋关节和膝关节置换术（AJJR, 2021）。与THR相关的一个重要临床问题是由植入物表面释放的金属颗粒和离子引起的毒性，这可能损害局部组织并可能扩散到远处器官，导致全身毒性。这种毒性受颗粒和离子的大小和浓度的影响，在肝脏中尤其重要，肝脏是人体的主要代谢器官，也是植入物积累的主要部位。在这项研究中，我们的目的是研究钴铬钼（CoCrMo）和钛（Ti）颗粒/离子（通常称为磨损产物）浓度增加对远端器官，特别是肝脏的肝毒性。我们发现这些颗粒和离子通过金属离子转运体和吞噬作用进入细胞，导致明显的细胞损伤，其中钛离子表现出最高的毒性水平，其次是钴离子和CoCrMo颗粒。我们的体外和体内联合研究支持这样的假设，即植入物中的金属颗粒和离子会造成肝细胞损伤的重大风险。这强调了解决THR患者植入物源性金属毒性的系统性影响的重要性。

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引用次数: 0

Regenerative potential of biopolymers - calcium phosphate osteogenic composites in the healing of an experimental defect of the parietal bone 生物聚合物-磷酸钙成骨复合材料在实验性顶骨缺损愈合中的再生潜力

Q3 Biochemistry, Genetics and Molecular Biology

Biomaterials and biosystems

Pub Date : 2025-05-04 DOI: 10.1016/j.bbiosy.2025.100112

Leonid Sukhodub, Oleksii Korenkov, Liudmyla Sukhodub, Mariia Kumeda

Given the problem of healing defects of the skull vault, this work is designed to compare in vivo on experimental animals the regenerative potential of osteogenic composite materials different in composition and properties: a) modified with nanostructured hydroxyapatite (HA) and Zn²⁺ ions biopolymer alginate (Alg) matrix (Alg_HA), and b) loaded with nanoparticles (NPs) brushite (dicalcium phosphate dihydrate, DCPD) biopolymer chitosan (CS) matrix (CS_DCPD). Regeneration of the parietal bone defect in rats was studied on the 90th and 140th day using light and electron microscopy. Starting from the 90th day, mature lamellar bone tissue formation was observed in the peripheral parts of both implants, which was not yet detected in animals of the control group (healing under the blood clot). The materials underwent resorption during the experiment and were replaced by regenerated tissues. The complete biocompatibility of both materials and the absence of signs of inflammation have been proven. Alg_HA implantation contributed to faster formation and maturation of bone tissue compared to CS_DCPD.

针对颅骨拱顶缺损的修复问题，本研究在实验动物体内比较了不同组成和性能的成骨复合材料的再生潜力：a)由纳米结构羟基磷灰石（HA）和Zn2+离子修饰的生物聚合物海藻酸盐（Alg）基质（Alg_HA）和b)负载纳米（NPs）刷石（二水合磷酸二钙，DCPD）生物聚合物壳聚糖（CS）基质（CS_DCPD）。采用光镜和电镜观察大鼠顶骨缺损第90天和第140天的再生情况。从第90天开始，两种种植体的外周部分均观察到成熟的板层骨组织形成，而对照组动物尚未发现这种情况（血凝块下愈合）。在实验过程中，材料被吸收，并被再生组织所取代。两种材料的完全生物相容性和无炎症迹象已被证明。与CS_DCPD相比，植入Alg_HA能更快地形成和成熟骨组织。

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引用次数: 0

Arterial Junctional Hemostasis without Compression: Evaluation of Visco-liquid Hemostats in Male Swine✰ 无压迫的动脉结缔组织止血：粘液止血剂在雄性猪✰中的应用评价

Q3 Biochemistry, Genetics and Molecular Biology

Biomaterials and biosystems

Pub Date : 2025-04-11 DOI: 10.1016/j.bbiosy.2025.100111

Michelle A. Tucci , Joseph D. Lichtenhan , Hamad A. Benghuzzi , Drew A. Hildebrandt

Trimethylpentyl polysilsesquioxane (POSS) gels containing kaolin and chitin promote clotting and stabilize and strengthen thrombi in vitro, and may offer a potential solution for treating non-compressible wounds. This study was designed to determine if the gels could stop bleeding in a junctional arterial hemorrhage model. Anesthetized male swine were instrumented for systemic arterial pressure (MAP) measurement and saline infusion. A femoral artery was punctured (2 × 6 mm), allowed to bleed freely for 45 s, and then either QuikClot gauze bandage (QC; n = 7), POSS-Kaolin (PK) or POSS-Chitin (PC) (40 ml; n = 7/group) applied with no external compression. Blood loss (BL) at 60 min post-treatment was greater in QC (1166±79 ml) than PK (188±74 ml; p < 0.0001) or PC (523±116 ml; p = 0.0001); BL in PC was greater than in PK (p = 0.03). Total BL (180 min) was higher in QC (1210±93 ml) than PK (475±85 ml, p < 0.001) or PC (632±133 ml; p = 0.002) and in PC vs PK (p = 0.008). Time to clot was not different between PK (3 ± 1) or PC (10±3 min), but was longer in QC (44±9 min) than PK or PC (p < 0.0001 vs PK, p < 0.0003 vs PC). MAP fell 40±3 mmHg in QC by 10 min post-injury (p < 0.0003), and remained below control. PC MAP fell 41±5 mmHg, but returned to control, and MAP did not change in PK. POSS in combination with kaolin or chitin provided hemorrhage control and systemic hemodynamic stability without compression. These results support the treatment concept that this new approach to hemostasis can be efficacious in treating non-compressible trauma wounds.

含有高岭土和几丁质的三甲基戊基聚硅氧烷（POSS）凝胶促进体外凝血，稳定和强化血栓，可能为治疗不可压缩性伤口提供潜在的解决方案。本研究旨在确定凝胶是否可以在结膜动脉出血模型中止血。麻醉后的公猪进行全身动脉压（MAP）测量和生理盐水输注。穿刺股动脉（2 × 6mm），让其自由出血45s，然后使用QuikClot纱布包扎(QC；n = 7)， poss -高岭土（PK）或poss -甲壳素（PC） (40 ml；N = 7/组)，无外压。治疗后60min， QC组失血量（1166±79 ml）大于PK组（188±74 ml）；p & lt;0.0001)或PC(523±116 ml；P = 0.0001)；PC组的BL大于PK组（p = 0.03）。QC组总BL（1210±93 ml）高于PK组（475±85 ml）， p <；0.001)或PC(632±133 ml；p = 0.002), PC vs PK （p = 0.008）。PK组（3±1）和PC组（10±3 min）到凝块的时间无显著差异，但QC组（44±9 min）比PK组和PC组(p <；0.0001 vs PK, p <；0.0003 vs PC)。损伤后10 min， QC MAP下降40±3 mmHg (p <；0.0003)，并保持在控制之下。PC MAP下降41±5 mmHg，但恢复到对照组，MAP在PK中没有变化。POSS与高岭土或几丁质联合使用可以控制出血和全身血流动力学稳定，无需压迫。这些结果支持治疗概念，这种新的止血方法可以有效地治疗不可压缩的创伤伤口。

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引用次数: 0

Corrigendum to “In vitro and in vivo assessment of a non-animal sourced chitosan scaffold loaded with xeno-free umbilical cord mesenchymal stromal cells cultured under macromolecular crowding conditions” [Biomaterials and Biosystems, Volume 16, December 2024, 100102] “在大分子拥挤条件下负载无异种脐带间充质间质细胞的非动物源壳聚糖支架的体外和体内评估”的更正[生物材料和生物系统，第16卷，2024年12月，100102]

Q3 Biochemistry, Genetics and Molecular Biology

Biomaterials and biosystems

Pub Date : 2025-03-01 DOI: 10.1016/j.bbiosy.2025.100106

Alessia Di Nubila , Meletios-Nikolaos Doulgkeroglou , Mehmet Gurdal , Stefanie H. Korntner , Dimitrios I. Zeugolis

引用次数: 0