BMC genomic data最新文献

Objectives: This study presents the whole-genome sequence of Streptococcus agalactiae 40LHNT and 10RH2NT, isolated from bovine milk with subclinical mastitis in the North-West Province of South Africa. The objective was to characterize the genomes to understand the antibiotic resistance and virulent potential of the isolates.

Data description: Genomic DNA of S. agalactiae strains was extracted and sequenced using the Oxford Nanopore MiniON platform. De novo assembly was carried out using Flye v. v2.9, resulting in an average draft genome size of 2.25 Mb for both strains, assembled into 4 contigs for strain 40LHNT and 91 contigs for strain 10RH2NT with an average GC content of 35.5% for both strains. Genome quality assessment revealed an average completeness of 92.64% and a contamination level of 1.6%. For both strains, the closest reference type strain was S. agalactiae NCTC8181 (accession GCA_900458965.1), showing an average nucleotide identity of 97%, thereby confirming species-level identity and close genetic relatedness. Annotation using the NCBI Prokaryotic Genome Annotation Pipeline identified 2,067 and 1977 protein-coding genes for strains 40LHNT and 10RH2NT, respectively..

Objectives: The bacterial genus Exiguobacterium includes many versatile species isolated from diverse environments. This draft genome can serve as a fundamental tool for subsequent studies, including the design of specific primers for strain identification, and as a basis for comparative genomics.

Data description: Herein, we report the draft genome sequence of Exiguobacterium acetylicum GM-14, which was isolated from a conventionally grown bean (Phaseolus vulgaris) field located in Culiacán, Sinaloa, Mexico. The nucleotide sequence of this genome was deposited into NCBI GenBank under accession number PRJNA 1,175,395. In this report, we introduce the entire genomic sequence of Exiguobacterium acetylicum GM-14, which is 3,139,301 bp long, with a 47.3% G + C content and 3,178 coding sequences (CDSs). The use of the Exiguobacterium acetylicum GM-14 genome to help analyse possible virulence genes will contribute to the development of control strategies.

Clinical trial number: Not applicable.

Aim: The purpose of this study was to discuss effects of exosome miRNA-369-3p on the biological behavior of trophoblast cells in gestational diabetes.

Materials and methods: Measuring miRNA-369-3p mRNA expression in Normal and GDM pregnant women by RT-qPCR assay; using high glucose to make GDM cell model; using CCK-8 and flow cytometry to evaluate cell proliferation andapoptosis; measuring cell invasion and migration by transwell and wound healing assay; IL-10 mRNA and protein expression by RT-qPCR and WB assay; correlation between miRNA-369-3p and IL-10 by Dual luciferase assay.

Results: Compared with normal pregnant women, miRNA-369-3p mRNA level significantly up-regulation in GDM pregnant women (P<0.001); with high glucose stimulating, compared with Normal group, the cell proliferation rate (78.30 ± 4.27%) significantly inhibited with cell apoptosis significantly increasing (24.55 ± 2.01%) (P<0.001), cell invasion (65.33 ± 3.65%) and migration (48.31 ± 4.18) activities significantly inhibited with IL-10 significantly down-regulation (P<0.001, respectively). With miRNA-369-3p inhibitor supplement, the cell activities were significantly improved with IL-10 increasing.

Conclusion: Exosome miRNA-369-3pknockdown had effects to improved high glucose induced HTR-8/Svneo cell biological activities by stimulating IL-10.

Coastal ecosystems along Nigeria's Gulf of Guinea face increasing pollution from industrial discharges and oil-related activities, posing serious threats to marine biodiversity. This study investigated detoxification gene expression cytochrome P450, aryl hydrocarbon receptor (AhR), and metallothionein (MT) in two sympatric croaker species, Pseudotolithus typus and Pseudotolithus elongatus, collected from Awoye, Ayetoro, and Idi-Ogba in Ondo State. Heavy metals and Polycyclic Aromatic Hydrocarbons (PAHs) were quantified in liver, gill, kidney, and muscle tissues, while gene expression was assessed using qPCR. Results revealed distinct spatial, seasonal, and tissue-specific bioaccumulation patterns, with higher contaminant loads during the dry season. The liver exhibited the highest total metal and PAH concentrations, followed by kidney, muscle, and gill tissues. P. elongatus accumulated higher contaminant levels than P. typus, particularly at Idi-Ogba, indicating greater anthropogenic influence. Gene expression analyses showed significant upregulation (p < 0.05) of AhR, cytochrome P450, and MT in liver and kidney tissues, correlating positively with contaminant concentrations. Regression models indicated strong associations between heavy metals and MT expression (R² = 0.999) and between PAHs and AhR/P450 expression (R² = 0.96-0.97). P. elongatus exhibited a stronger transcriptional response to organic pollutants, while P. typus responded more to metals, reflecting species-specific detoxification strategies. This study provides the first comparative molecular information into contaminant-induced stress in Pseudotolithus species, highlighting their potential as biomarkers for monitoring coastal pollution in the Gulf of Guinea.

Objectives: Thermophilic bacteria are valuable biotechnological resources. A thermophilic bacterium isolated from the Thai hot spring sediments was subjected to complete genome sequencing. Genome data will facilitate bacterial identification and comparative genomics within the Hydrogenophilus genus and provide insights into polyhydroxybutyrate biosynthesis pathways for industrial applications.

Data description: The H. thermoluteolus KS0102 genome comprises a single circular chromosome containing 2,136,028 bp and 61.70% GC. The genome was sequenced using Illumina and Oxford Nanopore technologies and assembled using Unicycler. It contained 1,848 CDS, 9 rRNAs, 49 tRNAs, and 1 CRISPR region. This dataset enables the investigation of polyhydroxybutyrate biosynthesis pathways and comparative genomic studies of thermophilic adaptation mechanisms in Hydrogenophilus species.