Current research in physiology最新文献

From molecular to physical function: The aging trajectory 从分子到物理功能：衰老轨迹。

IF 2.1 Q3 PHYSIOLOGY

Current research in physiology

Pub Date : 2025-01-01 DOI: 10.1016/j.crphys.2024.100138

Tom A.H. Janssen , Caroline V. Lowisz , Stuart Phillips

Aging is accompanied by a decline in muscle mass, strength, and physical function, a condition known as sarcopenia. Muscle disuse attributed to decreased physical activity, hospitalization, or illness (e.g. sarcopenia) results in a rapid decline in muscle mass in aging individuals and effectively accelerates sarcopenia. Consuming protein at levels above (at least 50–100% higher) the current recommended intakes of ∼0.8 g protein/kg bodyweight/d, along with participating in both resistance and aerobic exercise, will aid in the preservation of muscle mass. Physiological muscle adaptations often accompany the observable changes in physical independence an older adult undergoes. Muscle fibre adaptations include a reduction in type 2 fibre size and number, a loss of motor units, reduced sensitivity to calcium, reduced elasticity, and weak cross-bridges. Mitochondrial function and structure are impaired in relation to aging and are worsened with inactivity and disease states but could be overcome by engaging in exercise. Intramuscular connective tissue adaptations with age are evident in animal models; however, the adaptations in collagenous tissue within human aging are less clear. We know that the satellite muscle cell pool decreases with age, and there is a reduced capacity for muscle repair/regeneration. Finally, a pro-inflammatory state associated with age has detrimental impacts on the muscle. The purpose of this review is to highlight the physiological adaptations driving muscle aging and their potential mitigation with exercise/physical activity and nutrition.

衰老伴随着肌肉量、力量和身体功能的下降，这种情况被称为肌肉减少症。由于体力活动减少、住院治疗或疾病（如肌肉减少症）导致的肌肉废用会导致老年人肌肉量迅速下降，并有效地加速肌肉减少症。摄入高于（至少高出50-100%）当前推荐摄入量（每公斤体重/天~ 0.8克蛋白质）的蛋白质，同时参加阻力运动和有氧运动，将有助于保持肌肉质量。生理肌肉适应通常伴随着老年人身体独立性的可观察变化。肌纤维的适应包括2型纤维大小和数量的减少，运动单位的丧失，对钙的敏感性降低，弹性降低，以及弱的交叉桥。线粒体功能和结构受损与衰老有关，并且随着不活动和疾病状态而恶化，但可以通过参与运动来克服。在动物模型中，肌内结缔组织随年龄的变化是明显的；然而，胶原组织在人类衰老过程中的适应性尚不清楚。我们知道卫星肌细胞池随着年龄的增长而减少，并且肌肉修复/再生的能力降低。最后，与年龄相关的促炎状态对肌肉有不利影响。这篇综述的目的是强调驱动肌肉衰老的生理适应及其通过运动/身体活动和营养的潜在缓解。

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引用次数: 0

Metabolic effects of late-onset estradiol replacement in high-fat-fed ovariectomized mice

IF 2.1 Q3 PHYSIOLOGY

Current research in physiology

Pub Date : 2025-01-01 DOI: 10.1016/j.crphys.2025.100144

Alessandra Gonçalves da Cruz, Jessica Denielle Matos dos Santos, Ester dos Santos Alves, Anne Raissa Melo dos Santos, Bruna Fantini Trinca, Felipe Nunes de Camargo, Guilherme Fancio Bovolin, João Paulo Camporez

Background

Decreased estrogen levels in postmenopausal women negatively impact metabolic health. It is known that estradiol (E2) replacement can reverse this condition. However, there is no consensus on whether the effects mediated by E2 depend on the starting time of E2 replacement after menopause. We aimed to investigate the effects of different onset E2 treatments on glucose tolerance and metabolic parameters in high-fat-fed ovariectomized mice.

Material and methods

Eight-week-old female C57BL/6J mice were divided into three groups: SHAM, OVX, and E2, to evaluate three different time points of E2 replacement after ovariectomy: early (after 4 weeks), intermediate (after 12 weeks), and late replacement (after 20 weeks). E2 groups received treatment through subcutaneous pellets.

Results

E2 replacement improved the parameters analyzed independently of the time since ovariectomy, reducing body weight gain and fat mass, as well as increasing the percentage of lean mass. Glucose intolerance, fasting insulin, HOMA-IR, and cholesterol levels were also reduced after treatment with E2. In the liver, there was a decrease in triacylglycerol (TAG) deposition, with no difference in the expression of SREBP1 and ERα proteins. In the muscle, there was a decrease in TAG deposition. In periuterine adipose tissue, there was an increase in the expression of SREBP1, FASN, and SCD, with no difference in the expression of ERα.

Conclusions

Our findings reinforce the critical role of E2 in regulating both glucose and lipid metabolism and indicate that E2 action on metabolic health was not dependent on time since ovariectomy for the parameters analyzed.

背景绝经后妇女体内雌激素水平下降，会对代谢健康产生负面影响。众所周知，雌二醇（E2）替代品可以扭转这种状况。然而，关于雌二醇的作用是否取决于绝经后开始补充雌二醇的时间，目前尚未达成共识。我们旨在研究不同起始 E2 处理对高脂喂养卵巢切除小鼠葡萄糖耐量和代谢参数的影响：SHAM组、OVX组和E2组，以评估卵巢切除术后E2替代的三个不同时间点：早期（4周后）、中期（12周后）和晚期（20周后）。结果 E2 替代改善了自卵巢切除术后不同时间段的分析参数，减少了体重增加和脂肪量，并增加了瘦体重的百分比。使用 E2 治疗后，葡萄糖不耐受、空腹胰岛素、HOMA-IR 和胆固醇水平也有所降低。在肝脏中，三酰甘油（TAG）沉积减少，SREBP1 和 ERα 蛋白的表达没有差异。在肌肉中，TAG沉积减少。结论我们的研究结果加强了 E2 在调节葡萄糖和脂质代谢中的关键作用，并表明 E2 对代谢健康的作用与所分析参数的卵巢切除时间无关。

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引用次数: 0

Enhancing laboratory education through collaborative online international learning: A case study between USA and UK students

IF 2.1 Q3 PHYSIOLOGY

Current research in physiology

Pub Date : 2025-01-01 DOI: 10.1016/j.crphys.2025.100141

Matthew Allan Jones , Pika Miklavc , MaryAnne Stewart

Collaborative Online International Learning (COIL) has emerged in recent years as an effective and viable alternative to increase the international opportunities within taught curricula. Through recent innovations in online collaboration tools, and elevated demand for international opportunities, there has been a recent increase in the development of COIL opportunities globally across a range of health aligned disciplines. This style of learning has been described as positively contributing to the internationalisation of students and enhancement of their transferable skills. However, there have been no reported COIL opportunities described in the fields of laboratory medicine and biomedicine, likely due to the large practical elements associated with the subjects. This study therefore aims to develop a COIL opportunity that incorporates practical laboratory elements and evaluate the efficacy of this teaching and learning approach.

A laboratory-based COIL was developed and delivered across two days. Day one was composed of synchronous livestreamed laboratory demonstrations and practical activities, with day two focused on the completion of a time-dependent team-based task. The pedagogical impact of this laboratory-based COIL was evaluated through 1) pre and post surveys and 2) an overall survey utilising Likert scales.

The laboratory-based COIL was well received by students (n = 34) with the majority enjoying (94.1 %) and learnt a lot (94.1 %) by participating in the session. It also produced highly positive benefits to student confidence (97.1 %), teamworking (100 %), and communication (97.1 %). Pre (n = 46) and Post-analysis (n = 35) revealed significant enhancement of students international education knowledge, international medical practice knowledge, cultural intelligence, social initiative, emotional stability, and work-based flexibility (P < 0.05). Further analysis based on participants international institution revealed significant differences in responses between the two participating cohorts, namely questions relating to cultural intelligence and their confidence of working with cultures unfamiliar to themselves.

We are the first to report that implementation of laboratory-based COIL opportunities have significant potential in enhancing students' international, cultural and transferable competencies within laboratory and health education. These findings suggest that practical-based COILs are effective methods for preparing students to thrive in a globalized healthcare environment, making a strong case for its continued use and expansion in educational programmes.

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引用次数: 0

Assessment of the hypoglycemic effect of Cyanthillium cinereum (L.) H. Rob. and its dual impact on uterine contraction in gestational diabetic rats

IF 2.1 Q3 PHYSIOLOGY

Current research in physiology

Pub Date : 2025-01-01 DOI: 10.1016/j.crphys.2025.100139

Sasitorn Kerdsuknirund , Arreeya Kosinan , Panida Khunkaewla , Pakanit Kupittayanant , Pattama Tongdee , Porntip Nimkuntod , Susan Wray , Sajeera Kupittayanant

Objective

This study investigates the effects of Cyanthillium cinereum (L.) H. Rob. ethanolic extract (CCE) on gestational diabetes mellitus (GDM) in rats using biochemical, histological, and uterine contractility studies.

Methods

Diabetes was induced in pregnant rats using streptozotocin (60 mg/kg). CCE was administered orally at low (50 mg/kg BW) and high (500 mg/kg BW) doses from gestation day 7 to day 21. Maternal evaluations included body weight, gravid uterine weight, and biochemical assays for serum glucose, insulin, lipids, and liver enzymes. Fetal outcomes included fetal size. Histological analyses of maternal pancreatic and uterine tissues and uterine contractility studies using ex vivo muscle strip experiments were also performed.

Results

CCE and metformin (MET) significantly reduced elevated blood glucose levels and improved the Islets of Langerhans area compared to the GDM group (P < 0.05). Both treatments showed a trend toward increased insulin levels (P > 0.05) and significantly reduced lipids, AST, and ALP levels (P < 0.05). High-dose CCE and MET increased gravid uterine weight and fetal size (P < 0.05) while showing a trend toward reducing placental weight and index (P > 0.05). Histological analysis revealed increased fiber area and decreased interstitial space in uterine sections (P < 0.05). Ex vivo, CCE enhanced spontaneous and oxytocin-induced contractions (P < 0.05), while MET had no effect.

Conclusion

CCE reduces elevated glucose levels and exhibits hypolipidemic and hepatoprotective effects, improving maternal and fetal outcomes in GDM. Its uterine contractility effects suggest potential as a complementary therapy to MET for GDM management.

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引用次数: 0

Acidosis enhances contribution of Ca2+-activated chloride channels to vascular tone regulation in early postnatal period

IF 2.1 Q3 PHYSIOLOGY

Current research in physiology

Pub Date : 2025-01-01 DOI: 10.1016/j.crphys.2025.100143

Anastasia A. Shvetsova , Margarita A. Khlystova , Valentina S. Shateeva , Sofia D. Simonenko , Anna A. Borzykh , Denis V. Abramochkin , Dina K. Gaynullina

Introduction

Acidosis often occurs during clinical complications in newborns and can lead to changes in the mechanisms of arterial tone regulation. However, it is unknown how acidosis affects the activity of Ca²⁺-activated chloride channels (CaCC) in arteries during early ontogenesis. We hypothesized that their activity may increase during acidosis.

Methods

We studied isometric contractions of saphenous arteries isolated from adult and 10-13-day-old rats. Intracellular pH was measured using a fluorescent indicator BCECF-AM simultaneously with recording the contractile activity of the arterial preparation in isometric mode.

Results

Metabolic acidosis with pH = 6.8 caused a significant decrease in the arterial contractile responses of adult and 10-13-day-old rats. The functional contribution of CaCC was absent in the adult rat arteries both at pH = 7.4 and pH = 6.8. However, in 10-13-day-old rat pups, the functional contribution of CaCC was higher at pH = 6.8 compared to pH = 7.4.

Conclusion

Acidosis augments the functional role of CaCC in arteries during early postnatal ontogenesis, but not in adulthood.

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引用次数: 0

Microvascular endothelial cells display organ-specific responses to extracellular matrix stiffness

IF 2.1 Q3 PHYSIOLOGY

Current research in physiology

Pub Date : 2025-01-01 DOI: 10.1016/j.crphys.2025.100140

Rana Haidari , Wesley J. Fowler , Stephen D. Robinson , Robert T. Johnson , Derek T. Warren

The extracellular matrix was originally thought of as simply a cellular scaffold but is now considered a key regulator of cell function and phenotype from which cells can derive biochemical and mechanical stimuli. Age-associated changes in matrix composition drive increases in matrix stiffness. Enhanced matrix stiffness promotes the progression of numerous diseases including cardiovascular disease, musculoskeletal disease, fibrosis, and cancer. Macrovascular endothelial cells undergo endothelial dysfunction in response to enhanced matrix stiffness. However, endothelial cells are highly heterogeneous, adopting structural and gene expression profiles specific to their organ of origin. Endothelial cells isolated from different vessels (i.e. arteries, veins or capillaries) respond differently to changes in substrate stiffness. It is unknown whether microvascular endothelial cells isolated from different organs also display organ-specific responses to substrate stiffness. In this study, we compare the response of microvascular endothelial cells isolated from both the mouse lung and mammary gland to a range of physiologically relevant substrate stiffnesses. We find that endothelial origin influences microvascular endothelial cell response to substrate stiffness in terms of both proliferation and migration speed. In lung-derived endothelial cells, proliferation is bimodal, where both physiologically soft and stiff substrates drive enhanced proliferation. Conversely, in mammary gland-derived endothelial cells, proliferation increases as substrate stiffness increases. Substrate stiffness also promotes enhanced endothelial migration. Enhanced stiffness drove greater increases in migration speed in mammary gland-derived than lung-derived endothelial cells. However, stiffness-induced changes in microvascular endothelial cell morphology were consistent between both cell lines, with substrate stiffness driving an increase in endothelial volume. Our research demonstrates the importance of considering endothelial origin in experimental design, especially when investigating how age-associated changes in matrix stiffness drive endothelial dysfunction and disease progression.

{"title":"Microvascular endothelial cells display organ-specific responses to extracellular matrix stiffness","authors":"Rana Haidari , Wesley J. Fowler , Stephen D. Robinson , Robert T. Johnson , Derek T. Warren","doi":"10.1016/j.crphys.2025.100140","DOIUrl":"10.1016/j.crphys.2025.100140","url":null,"abstract":"<div><div>The extracellular matrix was originally thought of as simply a cellular scaffold but is now considered a key regulator of cell function and phenotype from which cells can derive biochemical and mechanical stimuli. Age-associated changes in matrix composition drive increases in matrix stiffness. Enhanced matrix stiffness promotes the progression of numerous diseases including cardiovascular disease, musculoskeletal disease, fibrosis, and cancer. Macrovascular endothelial cells undergo endothelial dysfunction in response to enhanced matrix stiffness. However, endothelial cells are highly heterogeneous, adopting structural and gene expression profiles specific to their organ of origin. Endothelial cells isolated from different vessels (i.e. arteries, veins or capillaries) respond differently to changes in substrate stiffness. It is unknown whether microvascular endothelial cells isolated from different organs also display organ-specific responses to substrate stiffness. In this study, we compare the response of microvascular endothelial cells isolated from both the mouse lung and mammary gland to a range of physiologically relevant substrate stiffnesses. We find that endothelial origin influences microvascular endothelial cell response to substrate stiffness in terms of both proliferation and migration speed. In lung-derived endothelial cells, proliferation is bimodal, where both physiologically soft and stiff substrates drive enhanced proliferation. Conversely, in mammary gland-derived endothelial cells, proliferation increases as substrate stiffness increases. Substrate stiffness also promotes enhanced endothelial migration. Enhanced stiffness drove greater increases in migration speed in mammary gland-derived than lung-derived endothelial cells. However, stiffness-induced changes in microvascular endothelial cell morphology were consistent between both cell lines, with substrate stiffness driving an increase in endothelial volume. Our research demonstrates the importance of considering endothelial origin in experimental design, especially when investigating how age-associated changes in matrix stiffness drive endothelial dysfunction and disease progression.</div></div>","PeriodicalId":72753,"journal":{"name":"Current research in physiology","volume":"8 ","pages":"Article 100140"},"PeriodicalIF":2.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143134703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The story so far………- current opinion in the use and applications of interactive storytelling in physiology and clinical education 迄今为止的故事.........--当前在生理学和临床教育中使用和应用互动故事的观点

IF 2.1 Q3 PHYSIOLOGY

Current research in physiology

Pub Date : 2025-01-01 DOI: 10.1016/j.crphys.2025.100142

Bagley L. , Wilson J. , Kime A.

Physiology and clinical practice are subjects of study which demand integration of multiple sources of systems working knowledge and information on the performance of those systems to come to meaningful conclusions. This is made more complex by the interpretation and actions as a result of this conclusion having direct impact on the sum of the component systems, the human, thereby integrating significant social and psychological considerations into an already complex situation.

As higher education educators, it is a significant challenge to provide our learners with training and most importantly, practice, in these knowledge, skills and behaviours in the classroom. There has been a significant interest in recent years in providing active learning opportunities which allow learners to apply subject knowledge to multi-faceted, immersive, continuously evolving stories which reflect a graduate's professional aspirations. This review highlights practices from the literature of storytelling education which the higher education educator can utilise in promoting “meaning making” in the classroom. Here, the case for interactive storytelling in physiology and clinical education is argued, as well as presenting commonly utilised techniques and practices with which educators can embed storytelling into their pedagogy as well as highlighting future directions in this field.

{"title":"The story so far………- current opinion in the use and applications of interactive storytelling in physiology and clinical education","authors":"Bagley L. , Wilson J. , Kime A.","doi":"10.1016/j.crphys.2025.100142","DOIUrl":"10.1016/j.crphys.2025.100142","url":null,"abstract":"<div><div>Physiology and clinical practice are subjects of study which demand integration of multiple sources of systems working knowledge and information on the performance of those systems to come to meaningful conclusions. This is made more complex by the interpretation and actions as a result of this conclusion having direct impact on the sum of the component systems, the human, thereby integrating significant social and psychological considerations into an already complex situation.</div><div>As higher education educators, it is a significant challenge to provide our learners with training and most importantly, practice, in these knowledge, skills and behaviours in the classroom. There has been a significant interest in recent years in providing active learning opportunities which allow learners to apply subject knowledge to multi-faceted, immersive, continuously evolving stories which reflect a graduate's professional aspirations. This review highlights practices from the literature of storytelling education which the higher education educator can utilise in promoting “meaning making” in the classroom. Here, the case for interactive storytelling in physiology and clinical education is argued, as well as presenting commonly utilised techniques and practices with which educators can embed storytelling into their pedagogy as well as highlighting future directions in this field.</div></div>","PeriodicalId":72753,"journal":{"name":"Current research in physiology","volume":"8 ","pages":"Article 100142"},"PeriodicalIF":2.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143594159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

How PPAR-alpha mediated inflammation may affect the pathophysiology of chronic kidney disease PPAR-α 介导的炎症如何影响慢性肾病的病理生理学

IF 2.1 Q3 PHYSIOLOGY

Current research in physiology

Pub Date : 2024-11-14 DOI: 10.1016/j.crphys.2024.100133

Sepiso K. Masenga , Selam Desta , Mark Hatcher , Annet Kirabo , Dexter L. Lee

Chronic kidney disease (CKD) is a major risk factor for death in adults. Inflammation plays a role in the pathogenesis of CKD, but the mechanisms are poorly understood. Peroxisome proliferator-activated receptor alpha (PPAR-α) is a nuclear receptor and one of the three members (PPARα, PPARβ/δ, and PPARγ) of the PPARs that plays an important role in ameliorating pathological processes that accelerate acute and chronic kidney disease. Although other PPARs members are well studied, the role of PPAR-α is not well described and its role in inflammation-mediated chronic disease is not clear. Herein, we review the role of PPAR-α in chronic kidney disease with implications for the immune system.

慢性肾脏病（CKD）是成年人死亡的主要风险因素。炎症在慢性肾脏病的发病机制中起着一定的作用，但对其机制却知之甚少。过氧化物酶体增殖激活受体α（PPAR-α）是一种核受体，也是 PPARs 三大成员（PPARα、PPARβ/δ 和 PPARγ）之一，在改善加速急性和慢性肾病的病理过程中发挥着重要作用。虽然对 PPARs 的其他成员进行了深入研究，但 PPAR-α 的作用尚未得到很好的描述，其在炎症介导的慢性疾病中的作用也不明确。在此，我们回顾了 PPAR-α 在慢性肾病中的作用以及对免疫系统的影响。

引用次数: 0

Cell-based homologous expression system for in-vitro characterization of environmental effects on transmembrane peptide transport in fish 基于细胞的同源表达系统，用于体外鉴定环境对鱼类跨膜肽转运的影响

Q3 PHYSIOLOGY

Current research in physiology

Pub Date : 2024-01-01 DOI: 10.1016/j.crphys.2024.100118

Pazit Con , Jens Hamar , Jakob Biran , Dietmar Kültz , Avner Cnaani

All organisms encounter environmental changes that lead to physiological adjustments that could drive evolutionary adaptations. The ability to adjust performance in order to cope with environmental changes depends on the organism's physiological plasticity. These adjustments can be reflected in behavioral, physiological, and molecular changes, which interact and affect each other. Deciphering the role of molecular adjustments in physiological changes will help to understand how multiple levels of biological organization are synchronized during adaptations. Transmembrane transporters, which facilitate a cell's interaction with its surroundings, are prime targets for molecular studies of the environmental effects on an organism's physiology. Fish are subjected to environmental fluctuations and exhibit different coping mechanisms. To study the molecular adjustments of fish transporters to their external surrounding, suitable experimental systems must be established. The Mozambique tilapia (Oreochromis mossambicus) is an excellent model for environmental stress studies, due to its extreme salinity tolerance. We established a homologous cellular-based expression system and uptake assay that allowed us to study the effects of environmental conditions on transmembrane transport. We applied our expression system to investigate the effects of environmental conditions on the activity of PepT2, a transmembrane transporter critical in the absorption of dietary peptides and drugs. We created a stable, modified fish cell-line, in which we exogenously expressed the tilapia PepT2, and tested the effects of water temperature and salinity on the uptake of a fluorescent di-peptide, β-Ala-Lys-AMCA. While temperature affected only Vmax, medium salinity had a bi-directional effect, with significantly reduced Vmax in hyposaline conditions and significantly increased Km in hypersaline conditions. These assays demonstrate the importance of suitable experimental systems for fish ecophysiology studies. Furthermore, our in-vitro results show how the effect of hypersaline conditions on the transporter activity can explain expression shifts seen in the intestine of saltwater-acclimated fish, emphasizing the importance of complimentary studies in better understanding environmental physiology. This research highlights the advantages of using homologous expression systems to study environmental effects encountered by fish, in a relevant cellular context. The presented tools and methods can be adapted to study other transporters in-vitro.

所有生物都会遇到环境变化，从而导致生理调整，进而推动进化适应。调整性能以应对环境变化的能力取决于生物的生理可塑性。这些调整可以反映在行为、生理和分子变化上，而这些变化是相互作用和相互影响的。破译分子调整在生理变化中的作用将有助于了解生物组织的多个层次在适应过程中是如何同步进行的。跨膜转运体可促进细胞与周围环境的相互作用，是研究环境对生物生理影响的分子研究的主要目标。鱼类会受到环境波动的影响，并表现出不同的应对机制。要研究鱼类转运体对外界环境的分子调节，必须建立合适的实验系统。莫桑比克罗非鱼（Oreochromis mossambicus）具有极强的耐盐性，是研究环境压力的绝佳模型。我们建立了一个基于细胞的同源表达系统和摄取检测方法，使我们能够研究环境条件对跨膜转运的影响。我们应用我们的表达系统研究了环境条件对 PepT2 活性的影响，PepT2 是一种跨膜转运体，对膳食肽和药物的吸收至关重要。我们创建了一个稳定的改良鱼细胞系，在其中外源表达罗非鱼 PepT2，并测试了水温和盐度对荧光二肽 β-Ala-Lys-AMCA 吸收的影响。温度只影响Vmax，而中盐度则具有双向影响，在低盐度条件下Vmax显著降低，而在高盐度条件下Km显著增加。这些实验证明了合适的实验系统对鱼类生态生理学研究的重要性。此外，我们的体外实验结果表明，低盐条件对转运体活性的影响可以解释盐水适应性鱼类肠道中的表达变化，强调了辅助研究对更好地理解环境生理学的重要性。这项研究强调了利用同源表达系统在相关细胞环境中研究鱼类所受环境影响的优势。介绍的工具和方法可用于体外研究其他转运体。

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引用次数: 0

Effect of a cajuína hydroelectrolytic drink on the physical performance and hydration status of recreational runners 水苏碱水电解饮料对休闲跑步者运动表现和水合状态的影响

Q3 PHYSIOLOGY

Current research in physiology

Pub Date : 2024-01-01 DOI: 10.1016/j.crphys.2024.100119

Valmir Oliveira Silvino , Mara Cristina Carvalho Batista , Manoel Miranda Neto , André Luiz Berzoti Ribeiro , Paulo Pedro do Nascimento , Esmeralda Maria Lustosa Barros , Rayane Carvalho de Moura , Karen Christie Gomes Sales , Luanne Morais Vieira Galvão , Lívio César Cunha Nunes , Alessandra Durazzo , Alexandre Sérgio Silva , Marcos Antonio Pereira dos Santos

Cajuína is a processed drink derived from cashew and is widely consumed in the northeast region of Brazil. This study evaluated the effect of a cajuína-based hydroelectrolytic drink on the aerobic performance and hydration status of recreational runners. Seventeen males (31.9 ± 1.6 years, 51.0 ± 1.4 ml/kg/min) performed three time-to-exhaustion running sessions on a treadmill at 70% VO₂max, ingesting cajuína hydroelectrolytic drink (CJ), high carbohydrate commercial hydroelectrolytic drink (CH) and mineral water (W) every 15 min during the running test. The participants ran 80.3 ± 8.4 min in CJ, 70.3 ± 6.8 min in CH and 71.8 ± 6.9 min in W, with no statistical difference between procedures. Nevertheless, an effect size of η² = 0.10 (moderate) was observed. No statistical difference was observed in the concentrations of sodium, potassium, and osmolality in both serum and urine between the three conditions. However, the effect size was moderate (urine sodium) and high (serum sodium, potassium, and osmolality). Urine specific gravity, sweating rate and heart rate were not significantly different between drinks. The cajuína-based hydroelectrolytic drink promotes similar effects compared to commercial hydroelectrolytic drink and water, considering specific urine gravity, heart rate, sweating, and time to exhaustion in recreational runners.

Cajuína 是一种从腰果中提取的加工饮料，在巴西东北部地区被广泛饮用。本研究评估了卡朱伊纳水电解饮料对休闲跑步者有氧运动表现和水合状态的影响。17 名男性（31.9±1.6 岁，51.0±1.4 毫升/千克/分钟）在跑步机上以 70% VO2max 的速度进行了三次耗时耗力跑步测试，在跑步测试期间每 15 分钟摄入一次水苏碱水电解饮料（CJ）、高碳水化合物商业水电解饮料（CH）和矿泉水（W）。参试者饮用 CJ 后的跑步时间为 80.3 ± 8.4 分钟，饮用 CH 后的跑步时间为 70.3 ± 6.8 分钟，饮用 W 后的跑步时间为 71.8 ± 6.9 分钟。不过，观察到的效应大小为 η2 = 0.10（中等）。在三种条件下，血清和尿液中的钠、钾和渗透压浓度均无统计学差异。然而，影响大小为中度（尿钠）和高度（血清钠、钾和渗透压）。不同饮料之间的尿比重、出汗率和心率没有显著差异。考虑到休闲跑步者的尿比重、心率、出汗率和体力耗尽时间，水苏碱水电解饮料与商业水电解饮料和水相比具有相似的效果。

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引用次数: 0