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Modification of pea protein isolates by high-intensity ultrasonication: Functional, structural and nutritional properties 用高强度超声波改变豌豆蛋白分离物:功能、结构和营养特性
Pub Date : 2024-08-19 DOI: 10.1016/j.focha.2024.100793
Gulay Ozkan , Pınar Tataroglu , Sukru Gulec , Esra Capanoglu

The current study aims to modify the functional, physical, structural and nutritional characteristics of pea protein isolate. High-intensity ultrasound treatment was used at 20 kHz frequency and 25 % amplitude for 10 (US10), 20 (US20), or 30 (US30) min. Results indicated that ultrasound application enhanced the protein solubility and zeta potential. When compared to control samples, the foaming capacity (FC) and stability (FS) as well as emulsion activity (EA) and stability (ES) were also increased from 157.5, 42.03, 46.25 and 53.75 % up to 182.5, 81.57, 72.50 and 67.50 %, respectively. Besides, particle size was found to be lower for ultrasound treated samples (92.9–131.1 nm) in comparison to that of untreated commercial pea protein isolate (161.9 nm). Moreover, while the bioaccessibility of pea protein in untreated sample was calculated as 28.90 %, ultrasonication increased the retention of pea protein up to 49.36 %. It can be concluded from the results that the ultrasonication process can be used as an advantageous, green and non-thermal tool for obtaining protein isolates with improved techno-functional properties and nutritional quality. Therefore, this treatment might improve the characteristics, and thus increase the utilization of plant-based proteins, especially pea protein, in various food systems.

本研究旨在改变豌豆分离蛋白的功能、物理、结构和营养特性。使用频率为 20 kHz、振幅为 25 % 的高强度超声波处理 10 (US10)、20 (US20) 或 30 (US30) 分钟。结果表明,超声波的应用提高了蛋白质的溶解度和 zeta 电位。与对照样品相比,起泡能力(FC)和稳定性(FS)以及乳液活性(EA)和稳定性(ES)也分别从 157.5%、42.03%、46.25% 和 53.75%提高到 182.5%、81.57%、72.50% 和 67.50%。此外,与未经处理的商用豌豆蛋白分离物(161.9 nm)相比,经超声波处理的样品的粒径更小(92.9-131.1 nm)。此外,据计算,未经处理的样品中豌豆蛋白的生物可及性为 28.90%,而超声波处理可将豌豆蛋白的保留率提高到 49.36%。从结果中可以得出结论,超声波处理可作为一种有利的、绿色的、非热处理的工具,用于获得具有更好的技术功能特性和营养质量的蛋白质分离物。因此,这种处理方法可以改善植物蛋白(尤其是豌豆蛋白)的特性,从而提高其在各种食品体系中的利用率。
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引用次数: 0
Enhancing probiotic survival and quality of fermented goat milk beverages with bael (Aegle marmelos) fruit pulp 用椴树果肉提高发酵羊奶饮料中益生菌的存活率和质量
Pub Date : 2024-08-15 DOI: 10.1016/j.focha.2024.100792
Jithmi Siriwardhana , D.M.D. Rasika , Dinusha Yapa , W.A.D.V. Weerathilake , Hasitha Priyashantha

This study aimed to assess the impact of bael fruit pulp on the viability of probiotic Lacticaseibacillus rhamnosus GG (LGG) and some physicochemical properties of bael-goat milk-based beverages during 21 days of refrigerated storage. Bael fruit pulp (BFP) was incorporated into goat milk (GM) at different levels (0 %, 5 %, 10 %, and 20 %), and fermented with LGG combined with conventional yoghurt culture. Products were analyzed at weekly intervals. Fermented GM without bael served as control. Redness (a*), yellowness (b*), chroma, and LGG viability were all significantly increased while pH, lightness (L*), and whiteness were dramatically decreased by the addition of BFP. After 14 days of storage, all fermented milk containing bael exhibited considerably (p < 0.05) greater LGG counts than the control. The product containing 20 % bael had the greatest viability counts (7.01 log CFU/mL) at the end of storage. Throughout the storage period, all of the products—including the control—maintained viable probiotic counts of greater than 6 log CFU/mL. The pH of the products decreased over time but was stabilized by bael. In conclusion, the findings demonstrate that goat milk is a perfect vehicle for LGG, and adding bael may boost the fermented goat milk's probiotic viability, physicochemical qualities, and nutritional value, thus improving its overall quality.

本研究旨在评估桦木果肉对益生菌鼠李糖乳杆菌(LGG)活力的影响,以及桦木果肉山羊奶饮料在21天冷藏储存期间的一些理化特性。在山羊奶(GM)中添加不同比例(0 %、5 %、10 % 和 20 %)的桦树果肉(BFP),并用 LGG 与传统酸奶培养物结合进行发酵。每周对产品进行一次分析。不含酵母的发酵转基因作为对照。红度(a*)、黄度(b*)、色度和 LGG 的活力都有显著提高,而 pH 值、亮度(L*)和白度则因添加 BFP 而显著降低。贮存 14 天后,所有含蝙蝠葛的发酵乳的 LGG 数量都大大高于对照组(p < 0.05)。贮藏结束时,含 20% 桦脂的产品的存活率最高(7.01 log CFU/mL)。在整个贮藏期间,所有产品(包括对照组)的益生菌存活计数都大于 6 log CFU/mL。产品的 pH 值会随着时间的推移而降低,但在贝尔的作用下会趋于稳定。总之,研究结果表明,羊奶是 LGG 的理想载体,添加桦胶可提高发酵羊奶的益生菌活力、理化质量和营养价值,从而改善其整体质量。
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引用次数: 0
Harnessing the health perks of underrated spices in bakery products- A review 在烘焙食品中利用被低估的香料的健康益处--综述
Pub Date : 2024-08-15 DOI: 10.1016/j.focha.2024.100790
Hassan Mehmood Sipra , Asad Ali , Qamar Abbas Syed , Muhammad Irfan , Syed Ali Hassan

The trend for eating foods which confer health benefits is increasing day by day. Spices are widely used in the world for adding flavor or aroma to different food products. Besides adding unique flavor and aroma to products several spices have been documented to possess functional and therapeutic abilities. Regular eating of these spices reduces the incidence of many diseases. Bakery products are consumed throughout the world and constitute one of the biggest food industries. Some spices that are not a part of our daily diet, have been incorporated into bakery products to enhance the nutritive and functional potential of the products as consumer demand is higher for more nutritious and health beneficial foods. The population is rising and the incidence of diseases is also increasing, so it has become necessary to add components like these spices for health benefits and to prevent the occurrence of many diseases. This review summarizes the functional and therapeutic potential of some underrated spices including rosemary, thyme, fenugreek, clove, cinnamon, oregano, and their incorporation to formulate the valuable bakery products.

食用有益健康的食品的趋势与日俱增。香料在世界上被广泛用于为不同的食品增添风味或香气。除了为产品增添独特的风味和香气外,一些香料还被证实具有功能性和治疗性。经常食用这些香料可以降低许多疾病的发病率。烘焙食品在世界各地都有消费,是最大的食品工业之一。由于消费者对营养和健康食品的需求越来越高,一些不属于我们日常饮食的香料被添加到烘焙食品中,以提高产品的营养和功能潜力。人口在不断增长,疾病的发病率也在不断上升,因此有必要添加这些香料等成分,以获得健康益处并预防多种疾病的发生。本综述总结了一些被低估的香料(包括迷迭香、百里香、葫芦巴、丁香、肉桂、牛至)的功能和治疗潜力,以及将它们用于配制有价值的烘焙产品的情况。
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引用次数: 0
Optimization of soya enriched burfi by using response surface methodology and its impact on biochemical parameters in albino rats 利用响应面方法优化富含大豆的布菲及其对白化大鼠生化指标的影响
Pub Date : 2024-08-15 DOI: 10.1016/j.focha.2024.100791
Tarun Verma, Jai Singh, Ankur Aggarwal

Increasing interest in soya based dairy products is growing due to their health benefits. This study aims to formulate soya-enriched burfi by varying levels of milk fat, soya cake powder (SCP), and sugar based on sensory and textural characteristics using response surface methodology. The optimised formulation consisting of 4.59 % milk fat, 19.24 % SCP, and 26.93 % sugar. Burfi formulations were developed with 10 % SCP (G3), 20 % SCP (G4), and control burfi (G2) which undergo analysis for chemical parameters (moisture, fat, protein, ash, total carbohydrate, free fatty acid, 5-hydroxymethylfurfural, thiobarbituric acid, and peroxide value), microbiological counts (total plate count, coliform, yeast & mold), growth metrics (FI, BWG & FE) and biochemical impact on albino rats fed with burfi (G3 and G4). Results showed that the soya-fed groups (G3 and G4) experienced less weight gain and an increase in albumin (4.05 %), globulin (5.33 %), and total protein (4.05 %), while levels of total cholesterol (5.36 %), triglycerides (5.17 %), creatinine (2.78 %), glucose (2.70 %), aspartate aminotransferase (8.15 %), and alanine transaminase (15.71 %) were reduced as compared to the control burfi group (G2). The study demonstrated that incorporating soya into burfi significantly enhances its nutritional and functional attributes, making it a promising functional food.

由于大豆对健康有益,人们对大豆乳制品的兴趣与日俱增。本研究旨在利用响应面方法,根据感官和质构特征,通过改变乳脂、豆饼粉(SCP)和糖的含量来配制富含大豆的布菲。优化配方包括 4.59 % 的乳脂、19.24 % 的豆饼粉和 26.93 % 的糖。开发了含 10 % SCP(G3)、20 % SCP(G4)和对照组 Burfi(G2)的 Burfi 配方,并对这些配方进行了化学参数(水分、脂肪、蛋白质、灰分、总碳水化合物、游离脂肪酸、5-羟甲基糠醛、硫代巴比妥酸和过氧化值)、微生物计数(总菌落总数、大肠菌群、酵母菌和霉菌)、生长指标(酵母菌和霉菌的生长指标、酵母菌和霉菌的生长指标、酵母菌和霉菌的生长指标、酵母菌和霉菌的生长指标、酵母菌和霉菌的生长指标)分析;霉菌)、生长指标(FI、BWG & FE)和生化影响。结果显示,喂食大豆的组(G3 和 G4)体重增加较少,白蛋白(4.05 %)、球蛋白(5.33 %)和总蛋白(4.05 %)增加,而总胆固醇(5.36 %)、甘油三酯(5.17 %)、肌酐(2.78 %)、葡萄糖(2.70 %)、天门冬氨酸氨基转移酶(8.15 %)和丙氨酸转氨酶(15.71 %)的水平与对照组(G2)相比有所降低。该研究表明,在布菲中加入大豆可显著提高其营养和功能属性,使其成为一种前景广阔的功能性食品。
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引用次数: 0
In vivo and in silico studies of membrane-stabilizing and clot lysis activities of Trachyspermum ammi 茵陈的膜稳定和凝块溶解活性的体内和硅学研究
Pub Date : 2024-08-13 DOI: 10.1016/j.focha.2024.100789
Md. Showkoth Akbor , Mst. Farjanamul Haque , Ahmmed Zunaed Rahman , Manik Chandra Shill , Hossam Kamli , Catarina Martins Tahim , Ivo Cavalcante Pita Neto , Henrique D.M. Coutinho , Muhammad Torequl Islam

Trachyspermum ammi contains many important phytochemicals, including thymol, which is used to treat many diseases. This study aimed to check the membrane-stabilizing and clot-lysis capacity of the ethanol extract of T. ammi (ETA) and its main chemical component, thymol (THY). For this, we used hypotonic solution-induced erythrocyte lysing and egg-albumin protein denaturation for membrane-stabilizing capacity and human blood clot lysis for anti-atherombosis capacity. Further, we observed in silico study of THY and ntric oxide synthase (NOSs) isoform. The stabilizing effects of ETA and THY on the membrane changed depending on the concentration. At the highest concentration (160 μg/mL), they stopped the most bleeding, while the standard drug, acetylsalicylic acid, stopped less bleeding at the same concentration. In the erythrocyte lysing test, ETA showed 102.60 ± 0.01 and 75.83 ± 1.44% membrane protection. In the egg-albumin denaturation test, they showed 27.62 ± 0.02% and 56.71 ± 0.02% membrane protection, respectively. In our clot lysis experiment, ETA and THY also showed significant and concentration-dependent clot lysis capacity. The IC50 and EC50 values of THY were lower than ETA when it came to keeping the membrane stable and breaking up clots, respectively. In our in silico investigation, THY and NOSs showed significant binding interaction. Taken together, ETA and its major component, THY, exhibited potent membrane stabilizing activity and clot lysis activity in in vitro studies. Further studies are required to elucidate its other active principles and their biological effects, along with possible mechanisms.

安米(Trachyspermum ammi)含有许多重要的植物化学物质,其中包括可用于治疗多种疾病的百里酚。本研究旨在检测安米的乙醇提取物(ETA)及其主要化学成分百里酚(THY)的膜稳定和凝血分解能力。为此,我们用低渗溶液诱导的红细胞裂解和鸡蛋白蛋白变性来检测膜稳定能力,用人血凝块裂解来检测抗动脉粥样硬化能力。此外,我们还对 THY 和一氧化氮合酶(NOSs)同工酶进行了硅学研究。ETA 和 THY 对膜的稳定作用随浓度的变化而变化。在最高浓度(160 微克/毫升)下,它们的止血效果最好,而在相同浓度下,标准药物乙酰水杨酸的止血效果较差。在红细胞裂解试验中,ETA 的膜保护率分别为 102.60 ± 0.01 和 75.83 ± 1.44%。在鸡蛋白蛋白变性试验中,它们的膜保护率分别为 27.62 ± 0.02% 和 56.71 ± 0.02%。在血凝块溶解实验中,ETA 和 THY 也显示出显著的浓度依赖性血凝块溶解能力。在保持膜稳定和分解凝块方面,THY 的 IC50 值和 EC50 值分别低于 ETA。在我们的硅学研究中,THY 和 NOSs 表现出明显的结合相互作用。综上所述,ETA 及其主要成分 THY 在体外研究中表现出了强大的膜稳定活性和凝块溶解活性。还需要进一步的研究来阐明其其他活性成分及其生物效应和可能的机制。
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引用次数: 0
Application of non-thermal plasma technology for enhancing food processing and storage: A Review 应用非热等离子体技术改进食品加工和储存:综述
Pub Date : 2024-08-08 DOI: 10.1016/j.focha.2024.100788
Udaya Vaka, MC Ramkumar

The food industry faces challenges as consumers aim for perceived health and less processed food. Non-thermal plasma technology is a unique strategy aimed at the food industry in this age of green technology. The primary goal of using non-thermal plasma processing is to preserve the purity of food products, while also improving their sensory attributes, nutritional and functional content. Non-thermal plasma has already demonstrated notable advantages in spore, enzyme, and toxin inactivation. Recent research examinations demonstrate that plasma processing has captured the interest of food processing and storage. The current review describes the process of non-thermal or cold plasma methods for food industrial benefits, such as improved cooking quality, increased activity of enzymes, starch modification, microbial elimination, and retaining the purity of food during storage.

由于消费者追求健康和少加工食品,食品工业面临着挑战。在这个绿色技术时代,非热等离子体技术是针对食品工业的一项独特战略。使用非热等离子体处理技术的主要目的是保持食品的纯度,同时改善其感官属性、营养和功能含量。非热等离子体在孢子、酶和毒素灭活方面已显示出显著的优势。最近的研究表明,等离子体处理已引起食品加工和储存领域的关注。本综述介绍了非热等离子体或冷等离子体加工方法对食品工业的益处,如改善烹饪质量、提高酶的活性、改良淀粉、消除微生物以及在贮藏过程中保持食品的纯度。
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引用次数: 0
Treatment and prevention of recurrent urolithiasis: Insights on molecular mechanism of occurrence and medical care 复发性尿路结石的治疗和预防:发生的分子机制和医疗护理的启示
Pub Date : 2024-07-26 DOI: 10.1016/j.focha.2024.100751
Arshi Khanam , Gurvirender Singh , Smita Narwal , Balram

Urolithiasis, commonly known as kidney stones, affects a significant portion of the global population. This disease raises the risk of urinary tract infections and kidney failure. According to the WHO study, the anticipated incidence ranges from 1 to 13 percent in different parts of the globe. The recurrence rate of kidney stones is significant. There are effective treatment options, preventative strategies are needed for both new and reoccurring stones to lessen the financial and bodily consequences of urolithiasis. Recent research shows that how common urolithiasis is in both genders changes over time. Males over 35 now have a greater lifetime risk of developing urolithiasis than they did previously. This increased risk is attributed to the influence of sex hormones on urolithiasis. A large majority of scientific and clinical urology research, especially related to renal conditions, concentrates on the diagnosis and treatment of the ailment, with minimal focus on its etiology or recurrence prevention. This study aimed to offer a holistic assessment of the causes and processes of stone formation, metabolic abnormalities, and particularly preventative ways of disease recurrence. This study also advances public understanding of the connection between urolithiasis and dietary, fluid intake, and lifestyle decisions.

泌尿系统结石,俗称肾结石,影响着全球相当一部分人口。这种疾病会增加尿路感染和肾衰竭的风险。根据世卫组织的研究,全球不同地区的预期发病率从 1%到 13%不等。肾结石的复发率很高。虽然有有效的治疗方案,但对于新发和复发的结石都需要采取预防策略,以减轻尿路结石对经济和身体造成的影响。最新研究表明,泌尿系结石在男女两性中的常见程度会随着时间的推移而发生变化。现在,35 岁以上的男性终生罹患尿路结石的风险比以前更高。这种风险的增加归因于性激素对尿路结石的影响。泌尿科的大部分科学和临床研究,尤其是与肾脏疾病有关的研究,都集中在该疾病的诊断和治疗上,而很少关注其病因或复发预防。这项研究旨在全面评估结石形成的原因和过程、代谢异常,尤其是预防疾病复发的方法。这项研究还有助于公众了解泌尿系结石与饮食、液体摄入量和生活方式之间的联系。
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引用次数: 0
Optimization & characterization of chips developed from cabbage powder, maize and rice flour using simplex lattice mixture design 利用简单网格混合物设计优化用卷心菜粉、玉米和大米粉制成的薯片并确定其特性
Pub Date : 2024-07-25 DOI: 10.1016/j.focha.2024.100787
Prachi Deshmukh , Dhanashree Patil , Prasanna P Bhalerao , Ashish Dabade , Sachin K Sonawane

This study employed a simplex lattice mixture design to optimize cabbage chips by blending cabbage powder, maize flour, and rice flour. Fourteen formulations were created using Design-Expert® software, with sensory attributes including crispiness, taste, appearance, aroma, and overall acceptability assessed. The approach revealed that cabbage powder (12 %), maize flour (10 %), and rice flour (27.97 %) effectively produced chips with desirable characteristics. Proximate analysis of the final chips (per 100 g) indicated 409.69 kcal energy, 70.97 g carbohydrates, 7.22 g protein, 10.77 g fat, 5.52 g total sugar, 4.04 % moisture, and 7.00 g ash. Mineral analysis revealed 9.48 mg calcium, 84.26 mg potassium, and 38.46 mg magnesium. This research optimizes cabbage chips, emphasizing their balanced nutrition and potential as a healthier snack.

本研究采用了简单网格混合物设计,通过混合卷心菜粉、玉米粉和大米粉来优化卷心菜片。使用 Design-Expert® 软件创建了 14 种配方,并对酥脆度、口感、外观、香气和总体可接受性等感官属性进行了评估。该方法显示,卷心菜粉(12%)、玉米粉(10%)和大米粉(27.97%)能有效生产出具有理想特性的薯片。最终薯片(每 100 克)的近似分析表明,能量为 409.69 千卡,碳水化合物为 70.97 克,蛋白质为 7.22 克,脂肪为 10.77 克,总糖为 5.52 克,水分为 4.04 %,灰分为 7.00 克。矿物质分析显示,钙含量为 9.48 毫克,钾含量为 84.26 毫克,镁含量为 38.46 毫克。这项研究对卷心菜片进行了优化,强调了其均衡的营养和作为更健康零食的潜力。
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引用次数: 0
Exploring the nutritional profiles and dietary value of indigenous fish species from the Mathabanga River in Bangladesh 探索孟加拉国马塔班加河土著鱼类的营养成分和膳食价值
Pub Date : 2024-07-23 DOI: 10.1016/j.focha.2024.100786
Mohajira Begum , Farzana Mim , Md. Selim Reza , Lailatul Ferdousi , Md. Sabir Hossain , Farha Matin Juliana , Md. Rakibul Hasan , Supriya Ahmed , Md. Zia Uddin Al Mamun , Md. Sujan Hossen , Rahima Akter Sathee , Ayan Goshwami

The study aimed to address the pervasive issue of inadequate nutrition in Bangladesh by analyzing eight distinct fish species, a pivotal step toward developing fish powder as a promising dietary supplement for nutritional enhancement. Samples from the Mathabhanga River in Chuadanga were rigorously processed and oven-dried to assess their nutritional properties, focusing on proximate composition, mineral content, and amino acid profile. Among the studied fish species, C. reba demonstrated the highest energy content (198.56 Kcal/100 g, p < 0.001), with notably elevated crude protein content (16.99 g/100 g, p < 0.001) compared to all except N. notopterus. The mineral composition order in fish powders was Ca > P > Fe > Na > Mg. Leucine emerged as the predominant essential amino acid (EAA) (6.84 to 32.15 mg/g, p < 0.001) among the species. O. pabo exhibited notably higher total EAA (∑EAA) levels (124.30 mg/g, p < 0.05) compared to other species. P. ticto showed a significantly higher ratio of total essential and non-essential amino acid content (∑EAA/∑NEAA) (0.41, p < 0.05) compared to other species. These findings underscored the nutritional value of the studied fish species, advocating their utilization as a dietary supplement to address malnutrition in Bangladesh.

该研究旨在通过分析八种不同的鱼类来解决孟加拉国普遍存在的营养不足问题,这是将鱼粉作为一种有前途的膳食补充剂来开发以增强营养的关键一步。对来自 Chuadanga 的 Mathabhanga 河的样本进行了严格处理和烘干,以评估其营养特性,重点是近似成分、矿物质含量和氨基酸谱。在所研究的鱼类物种中,C. reba 的能量含量最高(198.56 千卡/100 克,p < 0.001),粗蛋白含量(16.99 克/100 克,p < 0.001)显著高于除 N. notopterus 以外的所有鱼类。鱼粉中的矿物质组成顺序为 Ca > P > Fe > Na > Mg。亮氨酸是各物种中最主要的必需氨基酸(EAA)(6.84 至 32.15 mg/g,p < 0.001)。与其他物种相比,O. pabo 的总 EAA(∑EAA)水平明显更高(124.30 毫克/克,p < 0.05)。与其他物种相比,P. ticto 的必需氨基酸和非必需氨基酸总含量比(∑EAA/∑NEAA)明显更高(0.41,p < 0.05)。这些发现强调了所研究鱼类物种的营养价值,提倡将其作为膳食补充剂,以解决孟加拉国的营养不良问题。
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引用次数: 0
Preparation and characterization of Arctium lappa L. polysaccharide-Zn(II) complex and evaluation of its immune activity 牛蒡多糖-锌(II)复合物的制备、表征及其免疫活性评估
Pub Date : 2024-07-22 DOI: 10.1016/j.focha.2024.100784
Yang Liu , Wanbing Pan , Lingyu Li , Jianquan Gong , Hongjing Dong , Bin Zhang , Jiahao Lin , Zhenjia Zheng

A novel Arctium lappa L. polysaccharide zinc (ALP-Zn) complex was synthesized with success. The structural characteristics, stability, and in vitro digestive properties of ALP-Zn were studied. Moreover, the immunoreactivity of ALP-Zn on murine bone marrow-derived dendritic cells (BMDCs) was investigated by examining surface molecule expression, cytokine production and endocytosis. The results showed that the weight-average molecular weight (Mw) of ALP-Zn was 60,268 Da. ALP-Zn mainly consisted of fructose, arabinose, galactose, and glucose in a mass ratio of 50.74:27.94:12.49:7.26. Zn-O characteristic peak was identified by fourier transform infrared spectroscopy at 433.42 cm−1, indicating that zinc was successfully complexed with ALP. The stability and in vitro digestive properties suggested that ALP-Zn had good stability and biological acceptability. Furthermore, ALP and ALP-Zn demonstrated excellent immunological effects on dendritic cells (DCs). After being treated with ALP and ALP-Zn, CD80 and CD86 expression on the surface of DCs were increased and DCs maturation was induced. Moreover, ALP and ALP-Zn also significantly reduced DC endocytosis and increased cytokine (IFN-γ, TNF-α, IL-1β and IL-6). As a consequence, ALP-Zn complex could be as a potential zinc supplement with immune properties.

成功合成了一种新型牛蒡多糖锌(ALP-Zn)复合物。研究了 ALP-Zn 的结构特征、稳定性和体外消化特性。此外,还通过检测表面分子的表达、细胞因子的产生和内吞作用,研究了 ALP-Zn 在小鼠骨髓树突状细胞(BMDCs)上的免疫活性。结果表明,ALP-Zn 的平均分子量(Mw)为 60,268 Da。ALP-Zn 主要由果糖、阿拉伯糖、半乳糖和葡萄糖组成,质量比为 50.74:27.94:12.49:7.26。傅立叶变换红外光谱在 433.42 cm-1 处发现了 Zn-O 特征峰,表明锌与 ALP 成功络合。稳定性和体外消化特性表明,ALP-锌具有良好的稳定性和生物可接受性。此外,ALP 和 ALP-Zn 对树突状细胞(DCs)具有良好的免疫效应。经 ALP 和 ALP-Zn 处理后,树突状细胞表面的 CD80 和 CD86 表达增加,并诱导树突状细胞成熟。此外,ALP和ALP-Zn还能显著减少DC的内吞,增加细胞因子(IFN-γ、TNF-α、IL-1β和IL-6)。因此,ALP-锌复合物可作为一种具有免疫特性的潜在锌补充剂。
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Food chemistry advances
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