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Alleviation the effects of salinity stress using titanium dioxide nano and bulk particles in Echinacea seeds and seedlings 二氧化钛纳米颗粒和大块颗粒对紫锥菊种子和幼苗盐度胁迫的缓解作用
Q3 Agricultural and Biological Sciences Pub Date : 2021-10-29 DOI: 10.36253/ahsc-10850
Habibeh Behnam, H. Feizi, M. Alipanah
This study aimed to investigate the effect of nanoparticles and non-nanoparticles of titanium dioxide on germination indices of Echinacea under salinity stress. Experimental treatments included nano and bulk particles of titanium dioxide at concentrations of 0, 10, 50, 100 and 150 mg/l and salinity stress from NaCl at levels of 0, -3, -6 and -9 bar. The results showed that Echinacea is sensitive to high salinity stress levels (-6 and -9 bar). The use of nano and non-nano titanium dioxide treatment improved some traits under severe salinity stress. The germination percentage did not occur at salinity levels of -6 and -9 bar, but the addition of nano titanium dioxide with a concentration of 150 mg/l and 50 mg/l non-nano increased germination by 50.6%. Application of nano titanium dioxide increased the seedling weight in control by 1.28 mg to 4.26 mg in the treatment of 150 mg/l nanoparticles. The application of nano and bulk titanium dioxide could significantly reduce the negative effect of high salinity stress levels. This can be a valuable and hopeful solution to solve the problem of salinity stress in Echinacea.
本研究旨在研究纳米二氧化钛和非纳米二氧化钛在盐度胁迫下对紫锥菊发芽指数的影响。实验处理包括浓度为0、10、50、100和150 mg/l的二氧化钛纳米颗粒和大块颗粒,以及来自NaCl的盐度应力水平为0、-3、-6和-9巴。结果表明,紫锥菊对高盐度胁迫水平(-6和-9bar)敏感。纳米和非纳米二氧化钛处理改善了严重盐度胁迫下的一些性状。在-6和-9巴的盐度水平下,发芽率没有发生,但添加浓度为150毫克/升和50毫克/升的纳米二氧化钛使非纳米的发芽率提高了50.6%。在150毫克/l的纳米颗粒处理中,纳米二氧化钛使对照的幼苗重量增加了1.28毫克至4.26毫克。纳米二氧化钛和大块二氧化钛的应用可以显著减少高盐度应力水平的负面影响。这对解决紫锥菊的盐度胁迫问题是一个有价值和希望的解决方案。
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引用次数: 2
Strigolactone analogue GR24 reduces axillary bud out break and growth in tea tree, Melaleuca alternifolia (Maiden & Betche) Cheel Strigolactone类似物GR24减少茶树、互叶千层(Maiden&Betche)Cheel的腋芽脱落和生长
Q3 Agricultural and Biological Sciences Pub Date : 2021-10-21 DOI: 10.36253/ahsc-11519
G. Lowe, M. Shepherd, Terry J. Rose, C. Raymond
Strigolactone acts with other plant hormones to influence shoot architecture by suppressing axiliary bud outgrowth. The exogenous application of synthetic analogues of strigolactone, such as GR24, have been investigated as a way to manage plant architecture in a number of crops. In this study we test whether GR24 can be used to supress bud outgrowth in clonal propagules of tea tree (Melaleuca alternifolia) in order to retain a “single stem” form desirable for machine planting. GR24 was applied to decapitated rooted cuttings of tea tree at two rates (0.5 mg L-1 and 1.5 mg L-1), with and without auxin. By 21 days post-treatment, GR24 at both rates had significantly (p<0.05), reduced the mean number of axiliary buds (5.7 ± 0.4 and 5.5 ± 0.3 buds respectively) compared to decapitated untreated control plants (8.9 ± 0.6 buds). Suppression of buds was significantly higher again when auxin was applied in conjunction with GR24. Nonetheless, no exogenous hormone treatment was as effective at suppressing bud outgrowth as the apical dominance that occurred in intact control plants (1.1 ± 0.4 buds).
独角麦内酯与其他植物激素共同作用,通过抑制腋芽的生长来影响茎结构。己金内酯的合成类似物(如GR24)的外源应用已被研究作为一种管理许多作物植物结构的方法。在本研究中,我们测试了GR24是否可以用于抑制茶树(千层树)无性系繁殖体的芽生长,以保持机器种植所需的“单茎”形态。GR24以2种剂量(0.5 mg L-1和1.5 mg L-1)施用于茶树的无头根插条,添加和不添加生长素。处理21 d后,与未处理的对照(8.9±0.6芽)相比,GR24处理组的平均腋芽数(分别为5.7±0.4和5.5±0.3芽)显著减少(p<0.05)。当生长素与GR24同时施用时,对芽的抑制程度再次显著提高。尽管如此,没有任何外源激素处理在抑制芽生长方面能像完整对照植物(1.1±0.4个芽)的顶端优势那样有效。
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引用次数: 0
Hexachlamys edulis (Berg) Kausel & Legrand, “ubajay”, a native fruit species from South America Hexachlamys edulis (Berg) Kausel & Legrand, " ubajay ",南美洲的一种本地水果
Q3 Agricultural and Biological Sciences Pub Date : 2021-09-17 DOI: 10.36253/ahsc-10660
I. Povilonis, M. Arena, S. Radice
Hexachlamys edulis (Berg) Kausel & Legrand, “ubajay” is a native fruit species from South America belonging to the Myrtaceae family. Undoubtedly, it is a prominent species that provides potentially nutraceutical fruits, leaves with secondary metabolites of interest, and other organs with great benefits for human health and new alternatives for production systems. The aim of this work is to carry out a bibliographic review of all the scientific material published on this species to date. Research of this species could reveal and register its ethnobotanical potential.
Hexachlamys edulis (Berg) Kausel & Legrand,“ubajay”是一种来自南美洲的原生水果,属于桃金娘科。毫无疑问,它是一个突出的物种,提供潜在的营养保健果实,具有次级代谢物的叶子,以及对人类健康有很大好处的其他器官和生产系统的新替代品。这项工作的目的是对迄今为止发表的关于该物种的所有科学材料进行书目审查。本种的研究可以揭示和记录其民族植物学研究的潜力。
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引用次数: 4
Reflective benches for improving lighting in residential basil cultivation 用于改善住宅罗勒种植照明的反光长椅
Q3 Agricultural and Biological Sciences Pub Date : 2021-09-17 DOI: 10.36253/ahsc-10983
Daniele Ferreira Cavalcante, Eduardo Pradi Vendruscolo, A. Seleguini, Cássio De Castro Seron, E. Costa, Larissa Leandro Pires
Light-reflecting covers can help plant development in shaded environments, improving growing conditions in small residential spaces, encouraging the practice of small cultivations of food and ornamental species. Thus, the objective of this work was to evaluate the effect of reflective bench covers on the growth and development of different cultivars of basil, in pot and shaded environment. The treatments consisted of three reflective materials (aluminum thermal reflective mesh, shiny red and white coating on laminates), arranged on the cultivation benches, and two basil cultivars (green and purple). After 22 days under these environments, the number of leaves and nodes, stem diameter, plant height, relative chlorophyll content, fresh and dry masses of the aerial and roots parts were determined. The highest value of reflective photosynthetically active radiation was shown when using white laminate, followed by the red laminate and thermal reflective mesh. The green basil varieties showed higher values for number of leaves, stem diameter, plant height, fresh shoot weight and number of nodes, compared to the purple variety; however, in relation to the relative chlorophyll content, the purple variety was superior. The bright white and red laminate covers positively influence environmental conditions, increasing the reflectance of photosynthetically active radiation, and the development of basil plants in conditions of greater shading.
反光罩可以帮助植物在荫蔽环境中发育,改善小型住宅空间的生长条件,鼓励小型种植食物和观赏物种。因此,本工作的目的是评估反射台罩对不同品种罗勒在盆栽和荫蔽环境中生长发育的影响。处理由三种反射材料(铝热反射网、层压板上闪亮的红白涂层)和两种罗勒品种(绿色和紫色)组成,这些材料排列在培养台上。在这些环境下22天后,测定了地上部和根部的叶和节数、茎直径、株高、相对叶绿素含量、新鲜和干燥质量。当使用白色层压板时,反射性光合活性辐射的值最高,其次是红色层压板和热反射网。与紫色品种相比,绿色罗勒品种在叶数、茎径、株高、鲜梢重和节数方面表现出更高的值;然而,就相对叶绿素含量而言,紫色品种更为优越。明亮的白色和红色层压板覆盖物对环境条件产生了积极影响,增加了光合活性辐射的反射率,并在遮荫更大的条件下促进了罗勒植物的生长。
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引用次数: 0
Exogenous salicylic acid and ferulic acid improve growth, phenolic and carotenoid content in tomato 外源水杨酸和阿魏酸对番茄生长、酚类和类胡萝卜素含量有促进作用
Q3 Agricultural and Biological Sciences Pub Date : 2021-09-17 DOI: 10.36253/ahsc-8295
P. H. Gorni, Bianca da Silva Cornelissen, A. A. Pereira
Salicylic acid (SA) and ferulic acid (FA) are considered phenolic compounds that act as elicitors due to their regulatory functions on plant growth, development, metabolic and physiological responses in plants. The aim of this research was to evaluate the effect of SA and FA on growth, fruit quality and synthesis of secondary metabolites in tomato (Solanum lycopersicum cultivar Santa Clara). The experiment was conducted in pots in a greenhouse. The application of SA and FA was performed at concentration of 1.0 mmol L-1 alone and in combination, with water treated plants as control. Exogenous application of SA and FA either alone or in combination (SA + FA) resulted in increases in biomass accumulation and chlorophyll contents in tomato plant; and soluble sugar, total polyphenol, flavonoids, lycopene and β-carotene contents in fruits. It was concluded that application of SA and FA resulted in higher production and concentration of secondary compounds in tomato.
水杨酸(SA)和阿魏酸(FA)被认为是酚类化合物,由于其对植物生长、发育、代谢和生理反应的调节作用而起到诱导子的作用。本研究的目的是评估SA和FA对番茄生长、果实品质和次生代谢产物合成的影响。这个实验是在温室的花盆里进行的。SA和FA的施用以1.0mmol L-1的浓度单独和组合进行,水处理植物作为对照。SA和FA单独或联合施用(SA+FA)可增加番茄植株的生物量积累和叶绿素含量;以及果实中可溶性糖、总多酚、黄酮类化合物、番茄红素和β-胡萝卜素的含量。结果表明,SA和FA的施用提高了番茄次生化合物的产量和浓度。
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引用次数: 4
Influence of 6-benzylaminopurine spray time after pinching on growth and flowering of Veronica dahurica Steven 摘心后6-苄氨基嘌呤喷雾时间对白芷生长和开花的影响
Q3 Agricultural and Biological Sciences Pub Date : 2021-09-17 DOI: 10.36253/ahsc-10158
Jin-Ho Kim, H. Oh, Sang Yong Kim, G. Suh
Veronica dahurica Steven (family Scrophulariaceae) is an ornamental plant from Korea. The aim of the present study was to produce multiple-branched plants by using 6-benzylaminopurine (BA) spray application at several time intervals after pinching. All 10 cm long plants were sprayed with 0, 500, 1000, and 2000 mg·L-1 BA at 0, 7, and 14 days after pinching. Growth characteristics were examined 10 weeks after pinching and flowering time was recorded. The number of branches was highest in the group sprayed with 1000 mg·L-1 BA at 0 d after pinching. The greatest plant height was observed in the group treated with 1000 mg·L-1 BA 14 d after pinching and the minimum plant height was observed in the group sprayed with 500 mg·L-1 BA at 0 d after pinching. The groups sprayed with higher BA concentrations and with longer intervals between pinching and spraying showed greater delay in the time to first flower. The flower length was decreased in the pinched and BA-treated group compared with the control. Thus, BA application and pinching could promote multiple branch induction and control flowering time in V. dahurica Steven.
白芷(玄参科)是韩国的一种观赏植物。本研究的目的是通过在摘心后的几个时间间隔使用6-苄基氨基嘌呤(BA)喷雾来生产多分枝植物。在摘心后0、7和14天,用0、500、1000和2000mg·L-1 BA对所有10cm长的植株进行喷雾。在摘心10周后检查生长特性,并记录开花时间。1000 mg·L-1 BA组在摘心后0 d枝条数最高。1000 mg·L-1 BA处理组摘心后14d株高最高,500 mg·L-1BA处理组在摘心后0d株高最低。BA浓度越高、摘心和喷雾间隔越长的组,初花时间延迟越大。与对照组相比,摘心组和BA处理组的花朵长度减少。因此,施用BA和摘心可以促进白芷的多分枝诱导并控制开花时间。
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引用次数: 0
Comparison of wild and domesticated hot peppers fruit: volatile emissions, pungency and protein profiles 野生和驯化辣椒果实的比较:挥发性排放、辛辣和蛋白质特征
Q3 Agricultural and Biological Sciences Pub Date : 2021-08-03 DOI: 10.36253/ahsc-10630
Diego Comparini, C. Taiti, M. Lanza, Federico Vita, C. Pandolfi, S. Luti, F. Spinelli, L. Pazzagli, S. Mancuso
Capsicum plant species are globally cultivated in warm and temperate regions, being important for agro-economic, biological and cultural aspects. While their worldwide spread and their ability of cross-pollination to easily hybridize play an important role in the formation of numerous species and varieties but also create confusion for their classification. For this reason, the categorization of species and varieties is complex and several methods have been used to evaluate pepper plant origin and evolution. Therefore, the objectives of this study were to compare a wild pepper (Capsicum chacoense) with other two domesticated cultivars belonging to different species such as Capsicum annuum and C. baccatum and draw conclusions about their origins using different approaches. For this purpose three methodologies have been used and compared: the comparison of their fruits volatile organic compounds (VOCs) emissions , their capsaicin and dihydrocapsaicin content and the leaves proteomic profiles. The VOCs analysis has been conducted by a time-of-flight mass spectrometry (ToF-MS) with an innovative approach to better identify all the compounds detected, in particular using two different ionization agents (H3O+ and NO+) to better identify all the compounds detected. The VOCs and pungency analyses were then used to build back propagation neural networks (BPNN) and a Random Tree classifier to conduct a multivariate analysis and evaluate the most species-specific volatiles. The outcomes appeared to be a most accurate approach with respect to the traditional varieties descriptors used for peppers discrimination. The BPNN led to the identification of several putative volatiles as good candidates for the recognition of these species or significant nodes in a decision learning tool. Finally, protein profiles have been obtained by SDS-PAGE analysis on the leaves to perform a fast proteomic comparison among the species. The protein profiles showed the C. baccatum and C. chacoense were more similar to the domesticated pepper C. annuum.
辣椒属植物在全球暖温带地区种植,在农业经济、生物和文化方面具有重要意义。虽然它们在世界范围内的传播和容易杂交的异花授粉能力在众多物种和品种的形成中发挥了重要作用,但也给它们的分类带来了混乱。因此,物种和品种的分类很复杂,已经使用了几种方法来评估辣椒植物的起源和进化。因此,本研究的目的是将野生辣椒(Capsicum chacoense)与属于不同物种的其他两个驯化品种(如辣椒和辣椒)进行比较。baccatum,并使用不同的方法得出关于其起源的结论。为此,使用并比较了三种方法:果实挥发性有机化合物(VOCs)排放量、辣椒素和二氢辣椒素含量以及叶片蛋白质组学图谱的比较。挥发性有机物分析是通过飞行时间质谱法(ToF-MS)进行的,采用了一种创新的方法来更好地识别检测到的所有化合物,特别是使用两种不同的电离剂(H3O+和NO+)来更好地鉴定检测到的全部化合物。然后,使用挥发性有机物和刺激性分析来建立反向传播神经网络(BPNN)和随机树分类器来进行多元分析,并评估大多数物种特异性挥发物。与用于辣椒歧视的传统品种描述符相比,结果似乎是最准确的方法。BPNN将几种假定的挥发物识别为识别决策学习工具中这些物种或重要节点的良好候选者。最后,通过对叶片的SDS-PAGE分析获得了蛋白质图谱,以在物种之间进行快速蛋白质组学比较。蛋白质图谱显示,C.baccatum和C.chacoense与驯化辣椒C.annuum更相似。
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引用次数: 1
Biochemical changes in pear fruits during storage at ambient conditions 梨果实常温贮藏过程中的生化变化
Q3 Agricultural and Biological Sciences Pub Date : 2021-08-03 DOI: 10.36253/ahsc-10740
Avninder Kaur, Sucheta Sharma, N. Singh
‘Patharnakh’ (PN) (Pyrus pyrifolia Burm. Nakai) and ‘Punjab Beauty’ (PB) [Pyrus communis L. × Pyrus pyrifolia Burm. (Nakai)] are leading low-chill pear cultivars of subtropics of India. Diurnal temperature and relative humidity during fruit harvest period is high which considerably affect the shelf life of fruits. Fruits of ‘PN’ and ‘PB’ pear harvested at physiological maturity were stored for 12 days at ambient temperature and effects of storage temperature on physical and qualitative parameters were studied. Both cultivars showed reduction in fruit weight and firmness, reducing sugars, sucrose, starch and pectin content. However, total soluble solids and juice acid content increased during storage. Sucrose synthase activity and sucrose content showed significant positive correlation in ‘PN’ cultivar. Activities of fruit softening enzymes such as polygalacturonase (PG) and cellulase was enhanced; whereas, pectinmethylesterase (PME) was reduced during storage. Fruit firmness was negatively correlated with PG in both the cultivars. In ‘PN’ cultivar, fruit firmness was positively correlated with cellulase and negatively with PME enzyme but reverse trend was observed in ‘PB’ cultivar. Fruit minerals content didn’t show any substantial disparities in both the cultivars during storage. ‘Patharnakh’ and ‘Punjab Beauty’ fruits maintain desirable quality parameters up to 6-9 days and 3-6 days, respectively, during storage at ambient conditions.
“Patharnakh”(PN)(Pyrus pyrifolia Burm.Nakai)和“Punjab Beauty”(PB)【Pyrus commons L。水果收获期的昼夜温度和相对湿度较高,这对水果的保质期有很大影响。将生理成熟期收获的‘PN’和‘PB’梨果实在常温下贮藏12天,研究了贮藏温度对其物理和品质参数的影响。两个品种的果实重量和硬度、还原糖、蔗糖、淀粉和果胶含量都有所降低。然而,总可溶性固形物和果汁酸含量在储存过程中增加。‘PN’品种蔗糖合成酶活性与蔗糖含量呈显著正相关。果实软化酶如聚半乳糖醛酸酶(PG)和纤维素酶的活性增强;而果胶甲基酯酶(PME)在贮藏过程中降低。两个品种的果实硬度与PG呈负相关。在‘PN’品种中,果实硬度与纤维素酶呈正相关,与PME酶呈负相关,而在‘PB’品种中则相反。在贮藏过程中,两个品种的果实矿物质含量没有任何显著差异“Patharnakh”和“Punjab Beauty”水果在环境条件下储存期间,分别保持了6-9天和3-6天的理想质量参数。
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引用次数: 2
Investigation of modified WPM medium for the best meristem proliferation of Corylus avellana L. 改良WPM培养基对桔梗分生组织增殖效果的研究。
Q3 Agricultural and Biological Sciences Pub Date : 2021-08-03 DOI: 10.36253/ahsc-10536
Secil Kivrak Kiran, S. Galatali, Sevil Yeniocak, Damla Ekin Ozkaya, Taner Mercan, Sevinc Guldag, O. Çelįk, N. Abdul Ghafoor, Eergun Kaya
Cultivation of Corylus avellana L. in Turkey is performed generally in the northern regions where it is an important source of livelihood for the local farmers. More than 70% of world hazelnut production is supplied by Turkey, but compared with other countries, Turkey’s hazelnut production area is quite narrow. In this study was aimed to develop an effective in vitro production for seven local cultivars of C. avellana. Therefore, WPM medium supplemented with 6-Benzylaminopurine (BAP) was modified by using single or in combination of Fe-EDDHA, AgNO3, H3BO3, charcoal and gibberellic acid. In all varieties, the best regeneration rates varying between 68% and 94% were obtained from WPM medium supplemented with 4.4 µM BAP, 27.8 µM Fe-EDDHA and 10g/L Charcoal. Genetic stability of shoots derived from meristem culture using the best medium was analysed using ISSR primers, when the gel images of the PCR products were examined, no polymorphic band was observed in samples collected from seven provinces, and the genetic stability was determined as 100%.
土耳其的榛树种植主要集中在北部地区,是当地农民重要的生计来源。世界榛子产量的70%以上由土耳其供应,但与其他国家相比,土耳其的榛子生产面积相当狭窄。本研究旨在开发7个地方品种的有效离体生产技术。因此,采用Fe-EDDHA、AgNO3、H3BO3、木炭和赤霉素酸对添加6-苄基氨基嘌呤(BAP)的WPM培养基进行单独或联合改性。在WPM培养基中,添加4.4µM BAP、27.8µM Fe-EDDHA和10g/L木炭,再生率在68% ~ 94%之间。采用ISSR引物对最佳培养基分生组织培养的芽进行遗传稳定性分析,对PCR产物凝胶图像进行检测,在7个省采集的样品中均未发现多态性条带,遗传稳定性为100%。
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引用次数: 2
Genetic diversity and trait profiles of some Amaranthus genotypes 苋属植物部分基因型的遗传多样性及性状分析
Q3 Agricultural and Biological Sciences Pub Date : 2021-08-02 DOI: 10.36253/ahsc-10523
O. Oyetunde, M. O. Olayiwola, B. T. Osho
Knowledge of the pattern of trait variation among accessions, and the trait profiles of the accessions is crucial for improvement of a crop. Twenty-one Amaranthus accessions were evaluated in 2018 and 2019 to investigate the extent of genotypic diversity among the amaranth accessions and their trait profiles. Data were subjected to analysis of variance, and correlation and principal component analyses. Taking stem weight (SWT) as the yield, the accession × yield-trait combination (GYT) biplot was employed to investigate the trait profiles of the accessions. Accession, year, and accession × year mean squares were significant (P≤0.05/0.01) for most of the measured traits. The first three principal components explained 88.18% of observable variation among the accessions and identified plant height (PHT), number of leaves per plant (NOL), and root weight (RWT) as the major contributors. Significant (P≤0.01) correlation was observed in the association of SWT with NOL, TBM, and RWT. Accessions NGB00019 and NGB00061 were associated with the sector containing all the YT combinations considered. However, NGB00019 was identified as the best combiner of yield with other traits. Further studies involving more traits should determine the trait profiles of the remaining accessions.
了解种质间的性状变异规律和种质间的性状特征对作物改良具有重要意义。以2018年和2019年21份苋菜种质为研究对象,研究了不同种质间的基因型多样性程度及其性状特征。对数据进行方差分析、相关分析和主成分分析。以茎重(SWT)为产量,采用品种×产量性状组合(GYT)双标图对各品种的性状进行分析。大部分性状的加入、年份和加入×年均方差均显著(P≤0.05/0.01)。前3个主成分解释了88.18%的可观测变异,其中株高(PHT)、单株叶数(NOL)和根重(RWT)是主要贡献因子。SWT与NOL、TBM、RWT呈显著相关(P≤0.01)。编号NGB00019和NGB00061与包含所考虑的所有YT组合的扇区相关。而NGB00019是产量与其他性状的最佳组合。进一步的研究应涉及更多的性状,以确定剩余材料的性状特征。
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引用次数: 2
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Advances in horticultural science
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