Advances in medical sciences最新文献_第5页

Ropivacaine and lidocaine at clinically relevant concentrations suppress proliferation and migration of ovarian cancer cells and induce morphological alterations 临床相关浓度的罗哌卡因和利多卡因抑制卵巢癌细胞的增殖和迁移，并引起形态学改变。

IF 2.6 4区医学 Q3 MEDICINE, RESEARCH & EXPERIMENTAL

Advances in medical sciences

Pub Date : 2025-10-01 Epub Date: 2025-09-08 DOI: 10.1016/j.advms.2025.08.003

Jane M. Hayden , Andreas Tinnert , Kersti Alm , Jonatan Oras , Linda Block , Anil Gupta , Sven-Egron Thörn , Stina Oredsson

Purpose

Ovarian cancer ranks as a gynecological malignancy with poor prognosis, specifically if detected late. Primary treatment includes cytoreductive surgery and adjuvant chemotherapy with curative intent. Local anesthetics (LA) administered in the perioperative period may potentially impact patient outcome by several mechanisms. The beneficial impact of LA has been attributed, among other factors, to the drug's inhibitory effect on cancer cells. The primary aim of the study was to evaluate the effect of clinically relevant concentrations of ropivacaine and lidocaine on ovarian cancer cell lines.

Method

Three ovarian cancer cell lines (SKOV-3, SW-626 and CA-OV-3) were treated with 1, 10, 100, or 1000 μM of the two LAs. Cell function and morphology were assessed in the following ways: cell counting, phase-contrast and holographic microscopy, a conventional MTT assay for dose response testing, wound healing assay for migration, and cancer stem cell (CSC) identification by aldehyde dehydrogenase.

Results

Both ropivacaine and lidocaine significantly reduced cell number, altered morphology, suppressed migration, and decreased the population of CSCs in a concentration-dependent manner.

Conclusion

LAs exert a direct inhibitory effect on ovarian cancer cell lines in vitro, suggesting their potential benefits in perioperative management for patients undergoing surgery. Clinical studies using LA during ovarian cancer surgery are needed.

目的：卵巢癌是一种预后较差的妇科恶性肿瘤，尤其是发现较晚。主要治疗包括细胞减少手术和辅助化疗。围手术期局部麻醉（LA）的使用可能会通过几种机制影响患者的预后。除其他因素外，LA的有益作用归因于该药物对癌细胞的抑制作用。该研究的主要目的是评估临床相关浓度的罗哌卡因和利多卡因对卵巢癌细胞系的影响。方法：分别用1、10、100、1000 μM的两种LAs作用于3株卵巢癌细胞系SKOV-3、SW-626和CA-OV-3。通过以下方法评估细胞功能和形态：细胞计数，相对比和全息显微镜，常规MTT试验用于剂量反应测试，伤口愈合试验用于迁移，醛脱氢酶鉴定癌症干细胞（CSC）。结果：罗哌卡因和利多卡因均显著减少细胞数量，改变形态，抑制迁移，并以浓度依赖的方式减少CSC的数量。结论：LAs在体外对卵巢癌细胞系有直接抑制作用，提示其在手术患者围手术期管理中的潜在益处。在卵巢癌手术中使用LA的临床研究是必要的。

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引用次数: 0

Restoring impaired osteogenic differentiation of diabetic rat stromal cells using epigenetic inhibitors 利用表观遗传抑制剂恢复糖尿病大鼠基质细胞成骨分化受损。

IF 2.5 4区医学 Q3 MEDICINE, RESEARCH & EXPERIMENTAL

Advances in medical sciences

Pub Date : 2025-10-01 Epub Date: 2025-06-13 DOI: 10.1016/j.advms.2025.06.001

Mahshid Hodjat , Fazlullah Khan , Hadiseh Mohammadpour , Nasrin Asadi

Purpose

Epigenetic regulation plays a crucial role in gene expression and is recognized as a key contributor to diabetes-related complications. This study explores the osteogenic differentiation potential of stem cells isolated from the periodontal ligament (PDL) and bone marrow (BM) of diabetic rats. It investigates the effects of DNA methyltransferase and histone deacetylase inhibitors on the differentiation capacity of diabetic stem cells, searching for underlying mechanisms.

Method

Diabetes was induced in 5-week-old male Wistar rats using streptozotocin (STZ). Bone parameters were assessed via micro-CT, and stem cells isolated from mandibles and femurs were treated with 5-azacytidine or Trichostatin A in osteogenic medium. Osteogenic differentiation was evaluated through alkaline phosphatase (ALP) activity, Alizarin Red staining, and mRNA expression of osteogenic markers using real-time PCR.

Results

A significant decrease in total BMD and BV/TV of the femur and mandible was observed in STZ-induced diabetic rats compared to control. Cells isolated from diabetic PDL and BM showed impaired mineralization capacity and downregulated osteogenic markers. Treatment with Trichostatin A or 5-azacytidine restored mineralization potential, increased ALP activity, and upregulated the expression of RUNX2 and β-catenin.

Conclusion

Our results revealed the underlying epigenetic mechanisms responsible for the impaired osteogenic differentiation capacity of stem cells in diabetes. These findings highlight the potential of epigenetic modulators to restore stem cell function and enhance bone regeneration. This approach holds promise for improving diabetes-related skeletal complications and advancing tissue engineering strategies, including the development of scaffold-based therapies for fracture repair, periodontal regeneration, and implant integration in diabetic patients.

目的：表观遗传调控在基因表达中起着至关重要的作用，被认为是糖尿病相关并发症的关键因素。本研究探讨了糖尿病大鼠牙周韧带（PDL）和骨髓（BM）干细胞的成骨分化潜力。研究DNA甲基转移酶和组蛋白去乙酰化酶抑制剂对糖尿病干细胞分化能力的影响，寻找潜在的机制。方法：采用链脲佐菌素（STZ）诱导5周龄雄性Wistar大鼠患糖尿病。通过micro-CT评估骨参数，并在成骨培养基中用5-氮扎胞苷或曲古霉素A处理从下颌骨和股骨分离的干细胞。通过碱性磷酸酶（ALP）活性、茜素红染色和成骨标志物mRNA的实时PCR表达来评估成骨分化。结果：与对照组相比，stz诱导的糖尿病大鼠股骨和下颌骨的总骨密度和BV/TV明显降低。从糖尿病PDL和BM分离的细胞显示矿化能力受损和成骨标志物下调。曲古霉素A或5-氮杂胞苷处理恢复矿化电位，增加ALP活性，上调RUNX2和β-catenin的表达。结论：我们的研究结果揭示了糖尿病干细胞成骨分化能力受损的潜在表观遗传机制。这些发现强调了表观遗传调节剂在恢复干细胞功能和增强骨再生方面的潜力。这种方法有望改善糖尿病相关的骨骼并发症和推进组织工程策略，包括开发基于支架的治疗方法，用于骨折修复、牙周再生和糖尿病患者的种植体整合。

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引用次数: 0

Safety and efficacy of therapeutic plasma exchange in pediatric neuro-immunological diseases 血浆置换治疗小儿神经免疫疾病的安全性和有效性。

IF 2.5 4区医学 Q3 MEDICINE, RESEARCH & EXPERIMENTAL

Advances in medical sciences

Pub Date : 2025-03-01 Epub Date: 2025-01-15 DOI: 10.1016/j.advms.2025.01.003

Magdalena Błasiak , Przemysław Korohoda , Katarzyna Zachwieja , Dorota Drożdż , Aleksandra Gergont , Karina Madej-Świątkowska , Monika Miklaszewska

Purpose

Therapeutic plasma exchange (TPE) is the treatment of choice in various neuro-immunological disorders in pediatric populations. This study assesses the safety and efficacy of TPE in this demographic.

Materials and methods

A single-center, retrospective cohort study involving pediatric patients who experienced neuro-immunological events and underwent TPE procedures at a tertiary referral center was conducted.

Results

The study included 81 patients (Guillain-Barre: 65, polyneuropathies: 5, myasthenia gravis: 8, multiple sclerosis: 3), undergoing collectively 360 TPE procedures. Fresh frozen plasma (FFP) was used in 76.1 % of the TPE procedures. Adverse events (AEs) occurred in 50 % of TPEs using FFP compared to 39.5 % without. For procedures with at least two AEs, the rates were 24.5 % with FFP vs 8.1 % without. Incidence of allergic AEs was significantly higher in the FFP group (94.2 % of TPE with at least one AE) compared to those without FFP (47.2 %). Serious AE accounted for 1.2 % of TPE procedures and 2.5 % of patients. Effectiveness evaluations using a scale developed for this study and the Hughes Functional Grading Scale showed no significant clinical pre-treatment differences. After completing the treatment, children in the polyneuropathies group had the most severe clinical condition, and the largest relative improvement in clinical status was in the myasthenia gravis group.

Conclusions

TPE conducted by filtration is an effective and safe therapy for pediatric neuro-immunological diseases, with benefits outweighing the risks of complications. The use of FFP in therapy increases the probability of AE by 27 %, and significantly raises the risk of allergic and multiple AEs.

目的：治疗性血浆置换（TPE）是儿科人群中各种神经免疫疾病的治疗选择。本研究评估了TPE在这一人群中的安全性和有效性。材料和方法：一项单中心、回顾性队列研究，涉及在三级转诊中心经历神经免疫事件并接受TPE手术的儿科患者。结果：81例患者（吉兰-巴雷综合征65例，多发性神经病变5例，重症肌无力8例，多发性硬化症3例）共接受了360例TPE手术。76.1%的TPE手术采用新鲜冷冻血浆（FFP）。与未使用FFP的39.5%相比，使用FFP的tpe中有50%发生不良事件（ae）。对于至少有两次ae的手术，有FFP的发生率为24.5%，而没有FFP的发生率为8.1%。FFP组过敏性不良事件的发生率（94.2%）明显高于无FFP组（47.2%）。严重AE占TPE手术的1.2%，占患者的2.5%。使用为本研究开发的量表和Hughes功能分级量表进行的有效性评估显示，临床治疗前无显著差异。治疗结束后，多神经病变组患儿临床状况最严重，重症肌无力组患儿临床状况相对改善最大。结论：滤过式TPE治疗小儿神经免疫疾病是一种安全有效的治疗方法，其益处大于并发症的风险。使用FFP治疗可使AE的发生概率增加27%，并显著增加过敏和多发AE的发生风险。

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引用次数: 0

Evaluation of salusin-β in paediatric patients with chronic kidney disease or hypertension 慢性肾病或高血压患儿salusin-β的评价

IF 2.5 4区医学 Q3 MEDICINE, RESEARCH & EXPERIMENTAL

Advances in medical sciences

Pub Date : 2025-03-01 Epub Date: 2025-02-26 DOI: 10.1016/j.advms.2025.02.006

Mirjam Močnik , Sonja Golob Jančič , Martina Filipič , Evgenija Homšak , Mateja Svetej , Nataša Marčun Varda

Purpose

Salusins are newly identified endogenous peptides implicated in the atherosclerotic process. Salusin-β, in particular, is recognized for its proatherogenic role. Given that atherosclerosis can commence in childhood, salusin-β holds promise as a potential biomarker for cardiovascular risk assessment. The objective of our study was to investigate salusin-β levels in children with early stages of chronic kidney disease (CKD) or hypertension (HTN), and compare them to healthy controls. Furthermore, we aimed to evaluate its association with obesity and pulse wave velocity (PWV), the latter being a well-established marker for determining arterial elasticity.

Materials and methods

This cross-sectional study involved 96 paediatric patients, including 46 with CKD and 50 with HTN, as well as 33 healthy controls. Anthropometric measurements, PWV assessments, serum salusin-β values, and basic laboratory investigations were conducted for all participants.

Results

Salusin-β levels were found to be elevated in patients with CKD (p = 0.014), but not in patients with HTN when compared to healthy controls. When correlating salusin-β levels with PWV, a significant but weak correlation was observed (r = 0.211, p = 0.020).

Conclusions

Salusin-β levels were elevated in paediatric patients with CKD. Additionally, salusin-β levels correlated significantly with PWV. Obesity played a smaller role in these correlations, with significant correlations observed only after combining cardiovascular risk factors revealing certain associations between salusin-β levels and some cardiovascular variables, but with inconclusive findings and, in some instances, even contrary to anticipated outcomes.

目的alusins是新发现的与动脉粥样硬化过程有关的内源性肽。特别是Salusin-β，被认为具有促进动脉粥样硬化的作用。鉴于动脉粥样硬化可以在儿童时期开始，salusin-β有望成为心血管风险评估的潜在生物标志物。本研究的目的是调查早期慢性肾脏疾病（CKD）或高血压（HTN）儿童的salusin-β水平，并将其与健康对照进行比较。此外，我们旨在评估其与肥胖和脉搏波速度（PWV）的关系，后者是确定动脉弹性的公认标志。材料和方法本横断面研究纳入96例儿科患者，包括46例CKD患者和50例HTN患者，以及33例健康对照。对所有参与者进行了人体测量、PWV评估、血清salusin-β值和基础实验室调查。结果与健康对照相比，CKD患者的salusin -β水平升高（p = 0.014），而HTN患者的salusin -β水平没有升高。当salusin-β水平与PWV相关时，观察到显著但微弱的相关性（r = 0.211, p = 0.020）。结论儿童CKD患者salusin -β水平升高。此外，salusin-β水平与PWV显著相关。肥胖在这些相关性中所起的作用较小，只有在结合心血管危险因素揭示salusin-β水平与一些心血管变量之间的某些关联后，才能观察到显著的相关性，但结果不确定，在某些情况下，甚至与预期结果相反。

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引用次数: 0

To shock or not to shock - The accuracy of cardiac arrest rhythm assessment by paramedics in a simulated environment 电击还是不电击——护理人员在模拟环境中心脏骤停节律评估的准确性。

IF 2.5 4区医学 Q3 MEDICINE, RESEARCH & EXPERIMENTAL

Advances in medical sciences

Pub Date : 2025-03-01 Epub Date: 2024-12-18 DOI: 10.1016/j.advms.2024.12.002

Jerzy Jaskuła , Katarzyna Stolarz-Skrzypek , Klaudia Jaskuła , Jerzy Wordliczek , Grzegorz Cebula , Wojciech Zaręba , Małgorzata Kloch

Purpose

Defibrillation in shockable rhythm is a well-known key intervention in cardiopulmonary resuscitation (CPR). The aim of this study was to analyze accuracy (the sum of the numbers of true positive results and true negative results, divided by the number of total results) of deciding by paramedics whether the rhythm was shockable or non-shockable.

Methods

In this study 103 paramedics from various regions of Poland participated voluntarily. Study participants were presented with 22 simulated various electrocardiogram (ECG) recordings based on 10-s videos. These rhythms were also assessed using a manual defibrillator with shock-advisory mode known as automated external defibrillator (AED) mode.

Results

Among the 103 participants, the mean of correct answers (correct decision to defibrillate or correct decision not to defibrillate) was 18/22 (83.7 %). The highest possible score was achieved by the participant with 22/22 (100 %) correct answers, while the lowest was 10/22 (45.5 %). The highest score obtained for single rhythm was 97.1 % and the lowest was 32 %. Mean accuracy of shock-advisory mode was 77.3 %.

Conclusions

Improving the quality of paramedic training and continuous quality monitoring (e.g., by analyzing ECG recordings from resuscitations) is essential to improve the accuracy of defibrillation rhythm recognition. The role of the AED mode can be advisory, but is not a substitute for assessment by medical professionals in Emergency Medical Service.

目的：休克心律除颤是众所周知的心肺复苏（CPR）的关键干预措施。本研究的目的是分析护理人员判断心律是震荡还是非震荡的准确性（真阳性结果和真阴性结果的总和，除以总结果的数量）。方法：在本研究中，来自波兰不同地区的103名护理人员自愿参与。研究参与者观看了22个基于10秒视频的模拟心电图（ECG）记录。这些节律也使用具有休克咨询模式的手动除颤器进行评估，称为自动体外除颤器（AED）模式。结果：在103名参与者中，正确答案（正确决定除颤器或正确决定不除颤器）的平均值为18/22（83.7%）。满分为22/22(100%)，最低为10/22（45.5%）。单节律得分最高为97.1%，最低为32%。冲击预警模式的平均准确率为77.3%。结论：提高护理人员的培训质量和持续的质量监测（例如，通过分析复苏的心电图记录）对于提高除颤节律识别的准确性至关重要。AED模式的作用可以是咨询，但不能代替医疗专业人员在紧急医疗服务中的评估。

{"title":"To shock or not to shock - The accuracy of cardiac arrest rhythm assessment by paramedics in a simulated environment","authors":"Jerzy Jaskuła , Katarzyna Stolarz-Skrzypek , Klaudia Jaskuła , Jerzy Wordliczek , Grzegorz Cebula , Wojciech Zaręba , Małgorzata Kloch","doi":"10.1016/j.advms.2024.12.002","DOIUrl":"10.1016/j.advms.2024.12.002","url":null,"abstract":"<div><h3>Purpose</h3><div>Defibrillation in shockable rhythm is a well-known key intervention in cardiopulmonary resuscitation (CPR). The aim of this study was to analyze accuracy (the sum of the numbers of true positive results and true negative results, divided by the number of total results) of deciding by paramedics whether the rhythm was shockable or non-shockable.</div></div><div><h3>Methods</h3><div>In this study 103 paramedics from various regions of Poland participated voluntarily. Study participants were presented with 22 simulated various electrocardiogram (ECG) recordings based on 10-s videos. These rhythms were also assessed using a manual defibrillator with shock-advisory mode known as automated external defibrillator (AED) mode.</div></div><div><h3>Results</h3><div>Among the 103 participants, the mean of correct answers (correct decision to defibrillate or correct decision not to defibrillate) was 18/22 (83.7 %). The highest possible score was achieved by the participant with 22/22 (100 %) correct answers, while the lowest was 10/22 (45.5 %). The highest score obtained for single rhythm was 97.1 % and the lowest was 32 %. Mean accuracy of shock-advisory mode was 77.3 %.</div></div><div><h3>Conclusions</h3><div>Improving the quality of paramedic training and continuous quality monitoring (e.g., by analyzing ECG recordings from resuscitations) is essential to improve the accuracy of defibrillation rhythm recognition. The role of the AED mode can be advisory, but is not a substitute for assessment by medical professionals in Emergency Medical Service.</div></div>","PeriodicalId":7347,"journal":{"name":"Advances in medical sciences","volume":"70 1","pages":"Pages 51-56"},"PeriodicalIF":2.5,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142870971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mesenchymal stem cells versus mesenchymal stem cells-derived exosomes as potential autophagy pathway modulators in a diabetic model 间充质干细胞与间充质干细胞衍生的外泌体在糖尿病模型中作为潜在的自噬通路调节剂。

IF 2.5 4区医学 Q3 MEDICINE, RESEARCH & EXPERIMENTAL

Advances in medical sciences

Pub Date : 2025-03-01 Epub Date: 2025-02-14 DOI: 10.1016/j.advms.2025.02.004

Fatma Y. Meligy , Hanan Sharaf El-Deen Mohammed , Amal T. Abou Elghait , Heba K. Mohamed , Israa El-Sayed Mohamed Ashry , Ayat Abdel-Rahman Sayed , Ola A. Hussein , Ahmed Salman , Tarek Atia , Abir S. Mohamed , Nour H. Behnsawy , Safy Salah Gaber , Hader I. Sakr , Salwa Fares Ahmed

Purpose

This work compared the potential effects of bone marrow mesenchymal stem cells (BM-MSCs) with BM-MSCs-derived exosomes against impaired autophagy in streptozotocin (STZ)-induced diabetic rats.

Materials and methods

Three days after STZ injection, a single dose of (3 × 10^6) BM- MSCs or BM-MSCs-derived exosomes (80 μg/rat) was administered to evaluate their effects against nondiabetic and diabetic control rats. We assessed pancreatic structure via light and electron microscopy and evaluated its staining for insulin and the autophagy marker P62 immunohistochemically. Moreover, autophagy marker LC3 gene expression was examined by PCR.

Results

Both BM-MSCs and BM-MSCs derived exosomes showed histological restoration of pancreatic tissues. Both treatments markedly increased the amount of insulin and significantly decreased the autophagy markers P62 and LC3.

Conclusion

Our findings suggest that both BM-MSCs and BM-MSCs-derived exosomes provides a potential alternative to modulate diabetes mellitus.

目的：本研究比较了骨髓间充质干细胞（BM-MSCs）和BM-MSCs来源的外泌体对链脲佐菌素（STZ）诱导的糖尿病大鼠自噬受损的潜在作用。材料与方法：注射STZ后3 d，给予单剂量（3 × 10 × 6） BM-MSCs或BM-MSCs来源的外泌体（80 μg/大鼠），评估其对非糖尿病和糖尿病对照大鼠的作用。我们通过光镜和电镜评估胰腺结构，并评估其胰岛素和自噬标记物P62的免疫组织化学染色。采用PCR检测自噬标志物LC3基因的表达。结果：BM-MSCs和BM-MSCs衍生的外泌体均表现出胰腺组织的组织学恢复。两种处理均显著提高胰岛素水平，显著降低自噬标志物P62和LC3。结论：我们的研究结果表明，BM-MSCs和BM-MSCs衍生的外泌体都为调节糖尿病提供了一种潜在的选择。

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引用次数: 0

Optimizing in vitro lung cancer therapy with folate-conjugated polydopamine-coated liposomes loaded with gemcitabine 叶酸偶联多多巴胺包被脂质体负载吉西他滨优化肺癌体外治疗。

IF 2.5 4区医学 Q3 MEDICINE, RESEARCH & EXPERIMENTAL

Advances in medical sciences

Pub Date : 2025-03-01 Epub Date: 2025-02-07 DOI: 10.1016/j.advms.2025.02.001

Chandramohan Govindasamy , Muhammad Ibrar Khan , Chitrakani Bose , Muruganantham Bharathi , Shamini Senthilkumar , Parthasarathy Surya

Purpose

Surface-altered, targeted nanocarriers play crucial roles in chemotherapy. Incorporating ligands into polymers may alter their chemical composition, potentially compromising their drug storage and encapsulation capacity. Polydopamine (PDA) is a novel, biocompatible, and versatile agent for producing targeted nanoparticles that serve as a base for conjugating specific ligands to non-reactive polymeric nanocarriers. This investigation aimed to evaluate whether gemcitabine (GEM)-loaded liposomes conjugated with PDA could enhance cancer treatment.

Materials and methods

A series of liposomes, named plain GEM, GEM@FA, and GEM@FA/PDA, was designed. Transmission Electron Microscopy (TEM), Fourier Transform Infrared Spectroscopy (FTIR), and X-ray Photoelectron Spectroscopy (XPS) were used to confirm the presence of PDA coating and folic acid (FA) and PDA conjugations. Cellular uptake, cytotoxicity, and cell death were evaluated using biochemical and flow cytometric assays.

Results

Compared to typical liposomes, GEM@FA/PDA liposomes were smaller, more stable, and exhibited a spherical shape with excellent cellular uptake. GEM@FA and GEM@FA/PDA liposomes showed significantly higher cytotoxicity against lung cancer (H1299) cells compared to GEM liposomes and pure GEM solution at all concentrations, while causing much less cytotoxicity to normal cells (NIH3T3).

Conclusions

GEM@FA/PDA liposomes demonstrated enhanced cancer-fighting effectiveness while minimizing harm to healthy tissues, making them a promising approach for chemotherapy.

目的：表面修饰的靶向纳米载体在化疗中发挥着重要作用。将配体掺入聚合物可能会改变其化学组成，从而潜在地影响其药物储存和封装能力。聚多巴胺（PDA）是一种新型的、生物相容性的、多功能的药物，用于生产靶向纳米颗粒，作为将特定配体偶联到非反应性聚合物纳米载体的基础。本研究旨在评估负载吉西他滨（GEM）的脂质体与PDA结合是否可以增强癌症治疗。材料与方法：设计了一系列脂质体，分别命名为plain GEM、GEM@FA和GEM@FA/PDA。透射电镜（TEM）、傅里叶红外光谱（FTIR）和x射线光电子能谱（XPS）证实了PDA涂层以及叶酸（FA）和PDA偶联物的存在。使用生化和流式细胞术测定细胞摄取、细胞毒性和细胞死亡。结果：与典型脂质体相比，GEM@FA/PDA脂质体更小，更稳定，呈球形，具有良好的细胞摄取性。与GEM脂质体和纯GEM溶液相比，GEM@FA和GEM@FA/PDA脂质体在所有浓度下对肺癌（H1299）细胞的细胞毒性显著提高，而对正常细胞的细胞毒性要小得多（NIH3T3）。结论：GEM@FA/PDA脂质体显示出增强的抗癌效果，同时最大限度地减少对健康组织的伤害，使其成为一种有希望的化疗方法。

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引用次数: 0

Clinical value of CEUS with tumour marker monitoring in evaluating the prognosis of HCC after MWA 超声造影联合肿瘤标志物监测对肝癌MWA术后预后评价的临床价值。

IF 2.5 4区医学 Q3 MEDICINE, RESEARCH & EXPERIMENTAL

Advances in medical sciences

Pub Date : 2025-03-01 Epub Date: 2025-01-02 DOI: 10.1016/j.advms.2025.01.001

Qiang Guo , Yang Yu , Ruyun Ye , Zhiliang Huang , Tingting Shi

Purpose

This study aims to evaluate the prognostic value of contrast-enhanced ultrasound (CEUS) combined with tumour markers in patients with hepatocellular carcinoma (HCC) undergoing microwave ablation (MWA).

Methods

MWA patients with HCC were divided into good prognosis (n = 75) and poor prognosis (n = 69) groups. The levels of alpha-fetoprotein (AFP), carbohydrate antigen (CA19-9), and carcinoembryonic antigen (CEA) before and after MWA were analysed using an independent sample t-test. The correlation between prognosis, ablation lesion area, and tumour marker levels were analysed by Pearson's correlation. The diagnostic power of the ablation lesion area combined with tumour marker levels for the prognosis of patients with MWA was analysed using receiver operating characteristic (ROC) curves.

Results

The levels of AFP, CA19-9, and CEA in the good prognosis group were significantly lower than those in the poor prognosis group (all P < 0.001). The levels of all tumour markers were significantly negatively correlated with the prognosis of patients who underwent MWA (all r < 0, P < 0.001) and positively correlated with the area of tumour-ablated lesions (r > 0, P < 0.001). Moreover, the diagnostic efficacy of CEUS combined with tumour markers for the prognosis of patients who underwent MWA was significantly higher than that of either single diagnostic modality.

Conclusions

CEUS combined with tumour marker monitoring can effectively improve the accuracy of prognostic diagnosis in patients with MWA and provide a reference for timely and reasonable treatment and therapeutic evaluation.

目的：本研究旨在评价超声造影（CEUS）联合肿瘤标志物在肝细胞癌（HCC）微波消融（MWA）患者中的预后价值。方法：将MWA合并HCC患者分为预后良好组（n = 75）和预后不良组（n = 69）。采用独立样本t检验分析MWA前后甲胎蛋白（AFP）、碳水化合物抗原（CA19-9）、癌胚抗原（CEA）水平。采用Pearson相关分析预后、消融病灶面积与肿瘤标志物水平的相关性。采用受试者工作特征（ROC）曲线分析消融病灶面积结合肿瘤标志物水平对MWA患者预后的诊断能力。结果：预后良好组患者AFP、CA19-9、CEA水平显著低于预后不良组（P均< 0.001）。所有肿瘤标志物水平与MWA患者预后呈显著负相关（均r < 0, P < 0.001），与肿瘤消融灶面积呈正相关（均r < 0, P < 0.001）。此外，超声造影联合肿瘤标志物对MWA患者预后的诊断效果显著高于任何一种单一诊断方式。结论：超声造影联合肿瘤标志物监测可有效提高MWA患者预后诊断的准确性，为及时合理的治疗和疗效评价提供参考。

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引用次数: 0

Characteristics of patients with severe asthma reported to be eligible or non-eligible for biologic therapy in Poland: Results from the international observational study RECOGNISE 波兰报道的符合或不符合生物治疗条件的严重哮喘患者的特征：来自国际观察性研究recognition的结果

IF 2.5 4区医学 Q3 MEDICINE, RESEARCH & EXPERIMENTAL

Advances in medical sciences

Pub Date : 2025-03-01 Epub Date: 2025-03-17 DOI: 10.1016/j.advms.2025.03.003

Robert M. Mróz , Łukasz Minarowski , Jolanta Biegalska

Purpose

To analyze characteristics of real-world patients with severe asthma in Poland and identify factors determining clinicians’ opinion on the eligibility for biologics.

Methods

In this retrospective, multi-national, single-visit study (NCT03629782), investigators were asked whether the patient might benefit from a referral for further clinical assessment and potentially biologic therapy. Asthma exacerbations and healthcare resource utilization (HCRU) in the last 12 months were documented by the investigators. Patient-reported outcomes included St George's Respiratory Questionnaire (SGRQ) and Asthma Control Questionnaire (ACQ-6).

Results

Of 1025 patients, 146 were enrolled in Poland. Investigators considered 77.4 % of patients eligible for biologics and 22.6 % non-eligible. Patients considered eligible were diagnosed at younger age than non-eligible (median 39 vs 45 years; p = 0.0113) and more frequently had a history of atopy (46 % vs 21.2 %; p = 0.0106). Eligible patients had a higher frequency (93.8 % vs 66.7 %, p < 0.0001) and number of exacerbations (median 2 vs 1, p = 0.0003). Among eligible patients, 81.4 % and among non-eligible, 66.7 % had not well-controlled asthma; long-term oral corticosteroids were used by 46.0 % and 51.5 %, respectively. Short-term corticosteroid use was more frequent in biologics-eligible than in non-eligible patients (82.3 % vs 48.5 %, p < 0.0001). Patients eligible for biologics had a higher median SGRQ total score (64.7 vs 48.4; p = 0.0075) and HRCU (63.7 % vs 9.1 %; p < 0.0001).

Conclusions

In Poland, 3 in 4 patients with severe asthma are recognized by clinicians as potential candidates for biologics. However, eligibility is not associated with long-term oral corticosteroid use, highlighting the need to better inform the physicians about the benefits of biologic therapy.

目的：分析波兰现实世界严重哮喘患者的特征，并确定影响临床医生对生物制剂资格的因素。方法：在这项回顾性、多国、单次来访的研究（NCT03629782）中，研究人员被问及患者是否可能从进一步的临床评估和潜在的生物治疗中获益。研究人员记录了过去12个月的哮喘恶化和医疗资源利用（HCRU）。患者报告的结局包括圣乔治呼吸问卷（SGRQ）和哮喘控制问卷（ACQ-6）。结果：1025例患者中，146例在波兰入组。研究人员认为77.4%的患者符合使用生物制剂的条件，22.6%的患者不符合。被认为符合条件的患者比不符合条件的患者诊断年龄更小(中位39 vs 45岁；P =0.0113)，有特异反应史的患者较多(46% vs 21.2%；p = 0.0106)。符合条件的患者有更高的频率（93.8% vs 66.7%）。结论：在波兰，临床医生认为4例严重哮喘患者中有3例是生物制剂的潜在候选者。然而，适格性与长期口服皮质类固醇使用无关，强调有必要更好地告知医生生物治疗的益处。

{"title":"Characteristics of patients with severe asthma reported to be eligible or non-eligible for biologic therapy in Poland: Results from the international observational study RECOGNISE","authors":"Robert M. Mróz , Łukasz Minarowski , Jolanta Biegalska","doi":"10.1016/j.advms.2025.03.003","DOIUrl":"10.1016/j.advms.2025.03.003","url":null,"abstract":"<div><h3>Purpose</h3><div>To analyze characteristics of real-world patients with severe asthma in Poland and identify factors determining clinicians’ opinion on the eligibility for biologics.</div></div><div><h3>Methods</h3><div>In this retrospective, multi-national, single-visit study (NCT03629782), investigators were asked whether the patient might benefit from a referral for further clinical assessment and potentially biologic therapy. Asthma exacerbations and healthcare resource utilization (HCRU) in the last 12 months were documented by the investigators. Patient-reported outcomes included St George's Respiratory Questionnaire (SGRQ) and Asthma Control Questionnaire (ACQ-6).</div></div><div><h3>Results</h3><div>Of 1025 patients, 146 were enrolled in Poland. Investigators considered 77.4 % of patients eligible for biologics and 22.6 % non-eligible. Patients considered eligible were diagnosed at younger age than non-eligible (median 39 vs 45 years; p = 0.0113) and more frequently had a history of atopy (46 % vs 21.2 %; p = 0.0106). Eligible patients had a higher frequency (93.8 % vs 66.7 %, p < 0.0001) and number of exacerbations (median 2 vs 1, p = 0.0003). Among eligible patients, 81.4 % and among non-eligible, 66.7 % had not well-controlled asthma; long-term oral corticosteroids were used by 46.0 % and 51.5 %, respectively. Short-term corticosteroid use was more frequent in biologics-eligible than in non-eligible patients (82.3 % vs 48.5 %, p < 0.0001). Patients eligible for biologics had a higher median SGRQ total score (64.7 vs 48.4; p = 0.0075) and HRCU (63.7 % vs 9.1 %; p < 0.0001).</div></div><div><h3>Conclusions</h3><div>In Poland, 3 in 4 patients with severe asthma are recognized by clinicians as potential candidates for biologics. However, eligibility is not associated with long-term oral corticosteroid use, highlighting the need to better inform the physicians about the benefits of biologic therapy.</div></div>","PeriodicalId":7347,"journal":{"name":"Advances in medical sciences","volume":"70 1","pages":"Pages 203-208"},"PeriodicalIF":2.5,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143661896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Downregulation and inhibition of TRPM2 calcium channel prevent oxidative stress-induced endothelial dysfunction in the EA.hy926 endothelial cells model - Preliminary studies 下调和抑制TRPM2钙通道可预防EA.hy926内皮细胞模型中氧化应激诱导的内皮功能障碍——初步研究

IF 2.5 4区医学 Q3 MEDICINE, RESEARCH & EXPERIMENTAL

Advances in medical sciences

Pub Date : 2025-03-01 Epub Date: 2025-01-06 DOI: 10.1016/j.advms.2025.01.002

Wioletta Arendt , Klaudia Piekarska , Marta Hałas-Wiśniewska , Magdalena Izdebska , Alina Grzanka , Maciej Gagat

Purpose

Proper functioning of the endothelial barrier is crucial for cardiovascular system homeostasis. Oxidative stress can lead to endothelial dysfunction (ED), damaging lipids, proteins, and DNA. Reactive oxygen species also increase cytoplasmic Ca²⁺ levels, activating transient receptor potential melastatin 2 (TRPM2), a membrane non-selective calcium channel. The study aimed to assess TRPM2's significance in vascular endothelial cells' response to oxidative stress and the potential use of TRPM2 direct and indirect inhibitors in the prevention of oxidative stress-induced ED.

Materials and methods

EA.hy926 endothelial cells were exposed to hydrogen peroxide for 24 h to mimic oxidative stress conditions. To assess the significance of TRPM2 in the response of EA.hy926 cells to hydrogen peroxide TRPM2 siRNA as well as direct (N-(p-Amylcinnamoyl)anthranilic acid, flufenamic acid) and indirect (3-aminobenzamide, 3,4-dihydro-5[4-(1-piperidinyl)butyl]-1(2H)-isoquinolinone) TRPM2 inhibitors were tested.

Results

Results showed that hydrogen peroxide-induced ED is alleviated by TRPM2 downregulation. Moreover, preincubation of cells with both direct and indirect TRPM2 inhibitors for 30 min before hydrogen peroxide treatment reduces its negative effects on cell viability, cell migration, and junctional proteins.

Conclusions

The obtained results suggest that TRPM2 channel may be a potential target in therapy and prevention of cardiovascular diseases connected with oxidative stress-induced ED. However, further research is needed for clinical applications of direct and indirect TRPM2 inhibitors.

目的：内皮屏障的正常功能对心血管系统的稳态至关重要。氧化应激可导致内皮功能障碍（ED），损害脂质、蛋白质和DNA。活性氧也增加细胞质Ca2+水平，激活瞬时受体电位美拉抑素2 (TRPM2)，这是一种膜非选择性钙通道。本研究旨在评估TRPM2在血管内皮细胞对氧化应激反应中的意义，以及TRPM2直接和间接抑制剂在预防氧化应激诱导ed中的潜在应用。材料和方法：将EA.hy926内皮细胞暴露在过氧化氢环境中24小时，模拟氧化应激条件。为了评估TRPM2在EA.hy926细胞对过氧化氢TRPM2 siRNA以及直接（N-（对氨基肉桂基）苯甲酸、氟芬那酸）和间接（3-氨基苯甲酰胺、3,4-二氢-5[4-（1-哌替啶基）丁基]-1(2H)-异喹啉酮）TRPM2抑制剂反应中的意义。结果：过氧化氢诱导的ED可通过下调TRPM2得到缓解。此外，在过氧化氢处理前，将细胞与直接和间接TRPM2抑制剂一起预孵养30分钟，可以减少其对细胞活力、细胞迁移和连接蛋白的负面影响。结论：上述结果提示TRPM2通道可能是治疗和预防氧化应激性ED相关心血管疾病的潜在靶点，但TRPM2直接和间接抑制剂的临床应用还需进一步研究。

{"title":"Downregulation and inhibition of TRPM2 calcium channel prevent oxidative stress-induced endothelial dysfunction in the EA.hy926 endothelial cells model - Preliminary studies","authors":"Wioletta Arendt , Klaudia Piekarska , Marta Hałas-Wiśniewska , Magdalena Izdebska , Alina Grzanka , Maciej Gagat","doi":"10.1016/j.advms.2025.01.002","DOIUrl":"10.1016/j.advms.2025.01.002","url":null,"abstract":"<div><h3>Purpose</h3><div>Proper functioning of the endothelial barrier is crucial for cardiovascular system homeostasis. Oxidative stress can lead to endothelial dysfunction (ED), damaging lipids, proteins, and DNA. Reactive oxygen species also increase cytoplasmic Ca<sup>2+</sup> levels, activating transient receptor potential melastatin 2 (TRPM2), a membrane non-selective calcium channel. The study aimed to assess TRPM2's significance in vascular endothelial cells' response to oxidative stress and the potential use of TRPM2 direct and indirect inhibitors in the prevention of oxidative stress-induced ED.</div></div><div><h3>Materials and methods</h3><div>EA.hy926 endothelial cells were exposed to hydrogen peroxide for 24 h to mimic oxidative stress conditions. To assess the significance of TRPM2 in the response of EA.hy926 cells to hydrogen peroxide TRPM2 siRNA as well as direct (N-(p-Amylcinnamoyl)anthranilic acid, flufenamic acid) and indirect (3-aminobenzamide, 3,4-dihydro-5[4-(1-piperidinyl)butyl]-1(2H)-isoquinolinone) TRPM2 inhibitors were tested.</div></div><div><h3>Results</h3><div>Results showed that hydrogen peroxide-induced ED is alleviated by TRPM2 downregulation. Moreover, preincubation of cells with both direct and indirect TRPM2 inhibitors for 30 min before hydrogen peroxide treatment reduces its negative effects on cell viability, cell migration, and junctional proteins.</div></div><div><h3>Conclusions</h3><div>The obtained results suggest that TRPM2 channel may be a potential target in therapy and prevention of cardiovascular diseases connected with oxidative stress-induced ED. However, further research is needed for clinical applications of direct and indirect TRPM2 inhibitors.</div></div>","PeriodicalId":7347,"journal":{"name":"Advances in medical sciences","volume":"70 1","pages":"Pages 62-78"},"PeriodicalIF":2.5,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142942466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0