Advances in medical sciences最新文献_第3页

Investigation of the effects of umbilical cord and adipose-derived mesenchymal stem cells on endoplasmic reticulum stress in cadmium-induced rat kidney 脐带和脂肪源性间充质干细胞对镉诱导大鼠肾内质网应激影响的研究。

IF 2.6 4区医学 Q3 MEDICINE, RESEARCH & EXPERIMENTAL

Advances in medical sciences

Pub Date : 2025-07-26 DOI: 10.1016/j.advms.2025.07.002

Bahar Kartal , Uygar Saçik , Güven Erbil

Purpose

The hazardous heavy metal cadmium (Cd) has the potential to cause long-term kidney damage, mostly dependent on autophagy. Endoplasmic reticulum (ER) stress has been recognized as a primary source of Cd-induced toxicity. The ER chaperone GRP78 binds ER stress sensors, keeping them dormant. Exposure to Cd increases ER stress, a well-known inducer of autophagy. Adipose-derived mesenchymal stem cells (AD-MSC) are potentially useful tissue engineering and cellular treatment tools. Various disorders are treated with human umbilical cord MSCs (HUC-MSCs). They possess several unique qualities that are necessary for their therapeutic uses. The study aimed to investigate the effects of AD-MSCs and HUC-MSCs on Cd-induced nephrotoxicity.

Methods

The study used 36 male Wistar albino rats that were divided into six groups: control, AD-MSC, HUC-MSC, Cd, Cd + AD-MSC, and Cd + HUC-MSC. Hematoxylin and eosin (H&E) were used to stain the renal tissues in preparation for a histological analysis. Furthermore, the ER stress level was assessed by measuring GRP78 immunoexpression. Additionally, LC3B and Beclin-1 immunostaining were used to determine the autophagy.

Results

The histopathological results showed that the glomerular structure, proximal and distal tubules were disrupted in rat kidneys from the Cd group. Treatment with AD-MSCs and HUC-MSCs restored renal histological damage caused by Cd. Additionally, in Cd-induced renal tissues, there was an increase in the immunoexpression of the autophagic sensors LC3B and Beclin-1 and the ER stress indicator GRP78.

Conclusion

MSCs enabled Cd-damaged kidney tissues to regain an almost healthy histological structure.

目的：有害重金属镉（Cd）对肾脏有潜在的长期损害，主要依赖于自噬。内质网（ER）应激已被认为是cd诱导毒性的主要来源。内质网伴侣GRP78结合内质网应激传感器，使其处于休眠状态。暴露于Cd会增加内质网应激，这是一种众所周知的自噬诱导因子。脂肪源性间充质干细胞（AD-MSC）是潜在的有用的组织工程和细胞治疗工具。人类脐带间充质干细胞（HUC-MSCs）可治疗多种疾病。它们具有治疗用途所必需的一些独特品质。本研究旨在探讨AD-MSCs和HUC-MSCs对cd所致肾毒性的影响。方法：选用雄性Wistar白化大鼠36只，分为对照组、AD-MSC组、HUC-MSC组、Cd组、Cd+AD-MSC组、Cd+HUC-MSC组。用苏木精和伊红（H&E）染色肾组织，为组织学分析做准备。此外，通过检测GRP78的免疫表达来评估内质网应激水平。LC3B和Beclin-1免疫染色检测细胞自噬情况。结果：组织病理学结果显示，Cd组大鼠肾脏肾小球结构、近端小管和远端小管被破坏。用AD-MSCs和HUC-MSCs治疗可以恢复Cd引起的肾组织损伤。此外，在Cd诱导的肾组织中，自噬传感器LC3B和Beclin-1以及内质网络应激指标GRP78的免疫表达增加。结论：MSCs使cd损伤的肾脏组织恢复了近乎健康的组织结构。

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引用次数: 0

QT variability and myocardial repolarization in sleep apnea: implications for cardiac risk 睡眠呼吸暂停的QT变异性和心肌复极：对心脏风险的影响。

IF 2.6 4区医学 Q3 MEDICINE, RESEARCH & EXPERIMENTAL

Advances in medical sciences

Pub Date : 2025-07-26 DOI: 10.1016/j.advms.2025.07.004

Aleksandra Jarecka-Dobroń , Wojciech Braksator , Paweł Chrom

Purpose

Due to the increased risk of sudden cardiac death, we decided to assess ECG parameters related to the stability of the myocardial repolarization period, i.e. the corrected QT interval (QTc) and derivatives describing its variability (QTV and QTVi).

Methods

Healthy volunteers (n = 187) with visceral obesity, aged 35–65 years, were included. Each participant underwent Holter-ECG and polygraphy simultaneously. According to the severity of breathing disorders during sleep the cohort was divided into 3 groups: Respiratory Event Index (REI) 5–14/hour, REI 15–30/hour, REI ≥ 30/hour. The values of QT parameters were compared between obstructive sleep apnea (OSA)-positive and -negative group as well as among OSA positive group (depending on the OSA severity degree).

Results

We enrolled 121 patients, mean age 47.57 ± 9.36 (47 % female), mean BMI 32.18 ± 5.98 kg/m², 70 (58 %) of them were diagnosed with OSA, mean REI 26.79 ± 25.66/hour. In OSA group, QTV and QTVi were higher (p < 0.001) however QTc max was not significantly longer (p = 0.06).

Furthermore, we found significantly increased QTc max and QTVi in OSA positive patients during respiratory events compared to normal breathing (p = 0.02 and p = 0.008, respectively). Additionally, we found a positive correlation between REI and QTc max (p = 0.004, R = 0.22). Parameters related to hypoxia (oxygen desaturation index 4 %, time with SpO2 <90 %, SpO2 min, SpO2 mean) also presented a positive correlation with QTc max. Variables were not dependent on age or BMI.

Conclusions

Repolarization of cardiomyocytes is impaired in patients with OSA. The severity of impairment is positively correlated with the severity of sleep-related breathing disorders and hypoxemia.

目的：由于心源性猝死的风险增加，我们决定评估与心肌复极期稳定性相关的心电图参数，即校正QT间期（QTc）和描述其变异性的衍生物（QTV和QTVi）。方法：纳入年龄在35 ~ 65岁之间的内脏型肥胖健康志愿者187例。每位参与者同时进行动态心电图和测谎。根据睡眠中呼吸障碍的严重程度将队列分为3组：呼吸事件指数（REI） 5 ~ 14/h、REI 15 ~ 30/h、REI≥30/h。比较阻塞性睡眠呼吸暂停（OSA）阳性组和阴性组以及OSA阳性组（视OSA严重程度而定）QT间期参数值。结果：121例患者入组，平均年龄47.57±9.36（女性占47%），平均BMI 32.18±5.98 kg/m2，其中70例（58%）确诊为OSA，平均REI 26.79±25.66/h。结论：OSA患者心肌细胞复极功能受损。损害的严重程度与睡眠相关呼吸障碍和低氧血症的严重程度呈正相关。

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引用次数: 0

Prevalence of hepatitis D virus in chronic hepatitis B patients: findings from Poland 慢性乙型肝炎患者中丁型肝炎病毒的流行：来自波兰的调查结果

IF 2.6 4区医学 Q3 MEDICINE, RESEARCH & EXPERIMENTAL

Advances in medical sciences

Pub Date : 2025-07-25 DOI: 10.1016/j.advms.2025.07.003

Dorota Zarębska-Michaluk , Michał Brzdęk , Krystyna Dobrowolska , Diana Martonik , Anna Parfieniuk-Kowerda , Jakub Janczura , Kinga Brzdęk , Robert Pleśniak , Agnes Piszcz , Robert Flisiak

Purpose

This study aimed to determine the prevalence of hepatitis B/hepatitis D virus (HBV/HDV) co-infections in eastern Poland.

Materials/methods

We included consecutive patients with chronic hepatitis B treated with nucleos(t)ide analogues (NA) in the study, in whom we performed anti-HDV assays verified by molecular testing.

Results

The analysed population consisted of 398 patients, predominantly male with a median age of 50 years, 58.3 % of whom had comorbidities. Forty-three patients (10.8 %) were diagnosed with cirrhosis. Patients were treated with entecavir, tenofovir, or lamivudine for a median of 5 years. At the start of treatment, the median viral load was 5110 IU/ml, and 98 % of patients achieved viral eradication during the therapy. In the study population, anti-HDV antibodies were detected in 6 patients (1.5 %), 3 men and 3 women, of whom in 1 (0.25 %) the presence of genetic material of the HDV was confirmed by molecular testing.

Among anti-HDV-positive patients, 3 (50 %) had cirrhosis, 1 of them underwent liver transplantation, and 2 had extrahepatic malignancy. All of them were negative for hepatitis B virus envelope (HBe) antigen and had antibodies to HBe antigen present at the beginning of antiviral treatment. All anti-HDV-positive patients achieved viral clearance during the therapy.

Conclusion

In a population of almost four hundred patients infected with HBV, we observed a low prevalence of anti-HDV antibodies of 1.5 % and HDV replication of only 0.25 %. Half of the 6 patients with serologic evidence of co-infection had cirrhosis, so HDV likely played a role in disease progression.

目的：本研究旨在确定波兰东部乙型肝炎/丁型肝炎病毒（HBV/HDV）合并感染的患病率。材料/方法我们在研究中纳入了连续接受核苷类似物（NA）治疗的慢性乙型肝炎患者，我们对他们进行了抗hdv检测，并通过分子检测进行了验证。结果分析人群包括398例患者，以男性为主，中位年龄50岁，58.3%的患者有合并症。43例（10.8%）被诊断为肝硬化。患者接受恩替卡韦、替诺福韦或拉米夫定治疗的中位时间为5年。在治疗开始时，中位病毒载量为5110 IU/ml， 98%的患者在治疗期间实现了病毒根除。在研究人群中，6名患者（1.5%）检测到抗HDV抗体，其中3名男性和3名女性，其中1名（0.25%）通过分子检测证实存在HDV遗传物质。抗hiv阳性患者中，肝硬化3例（50%），肝移植1例，肝外恶性肿瘤2例。所有患者乙型肝炎病毒包膜（HBe）抗原呈阴性，抗病毒治疗开始时均有HBe抗原抗体。在治疗期间，所有抗hiv阳性患者都实现了病毒清除。结论在近400名感染HBV的患者中，我们观察到抗HDV抗体的低患病率为1.5%，HDV复制率仅为0.25%。有血清学证据的6例合并感染患者中有一半患有肝硬化，因此HDV可能在疾病进展中起作用。

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引用次数: 0

Restoring impaired osteogenic differentiation of diabetic rat stromal cells using epigenetic inhibitors 利用表观遗传抑制剂恢复糖尿病大鼠基质细胞成骨分化受损。

IF 2.5 4区医学 Q3 MEDICINE, RESEARCH & EXPERIMENTAL

Advances in medical sciences

Pub Date : 2025-06-13 DOI: 10.1016/j.advms.2025.06.001

Mahshid Hodjat , Fazlullah Khan , Hadiseh Mohammadpour , Nasrin Asadi

Purpose

Epigenetic regulation plays a crucial role in gene expression and is recognized as a key contributor to diabetes-related complications. This study explores the osteogenic differentiation potential of stem cells isolated from the periodontal ligament (PDL) and bone marrow (BM) of diabetic rats. It investigates the effects of DNA methyltransferase and histone deacetylase inhibitors on the differentiation capacity of diabetic stem cells, searching for underlying mechanisms.

Method

Diabetes was induced in 5-week-old male Wistar rats using streptozotocin (STZ). Bone parameters were assessed via micro-CT, and stem cells isolated from mandibles and femurs were treated with 5-azacytidine or Trichostatin A in osteogenic medium. Osteogenic differentiation was evaluated through alkaline phosphatase (ALP) activity, Alizarin Red staining, and mRNA expression of osteogenic markers using real-time PCR.

Results

A significant decrease in total BMD and BV/TV of the femur and mandible was observed in STZ-induced diabetic rats compared to control. Cells isolated from diabetic PDL and BM showed impaired mineralization capacity and downregulated osteogenic markers. Treatment with Trichostatin A or 5-azacytidine restored mineralization potential, increased ALP activity, and upregulated the expression of RUNX2 and β-catenin.

Conclusion

Our results revealed the underlying epigenetic mechanisms responsible for the impaired osteogenic differentiation capacity of stem cells in diabetes. These findings highlight the potential of epigenetic modulators to restore stem cell function and enhance bone regeneration. This approach holds promise for improving diabetes-related skeletal complications and advancing tissue engineering strategies, including the development of scaffold-based therapies for fracture repair, periodontal regeneration, and implant integration in diabetic patients.

目的：表观遗传调控在基因表达中起着至关重要的作用，被认为是糖尿病相关并发症的关键因素。本研究探讨了糖尿病大鼠牙周韧带（PDL）和骨髓（BM）干细胞的成骨分化潜力。研究DNA甲基转移酶和组蛋白去乙酰化酶抑制剂对糖尿病干细胞分化能力的影响，寻找潜在的机制。方法：采用链脲佐菌素（STZ）诱导5周龄雄性Wistar大鼠患糖尿病。通过micro-CT评估骨参数，并在成骨培养基中用5-氮扎胞苷或曲古霉素A处理从下颌骨和股骨分离的干细胞。通过碱性磷酸酶（ALP）活性、茜素红染色和成骨标志物mRNA的实时PCR表达来评估成骨分化。结果：与对照组相比，stz诱导的糖尿病大鼠股骨和下颌骨的总骨密度和BV/TV明显降低。从糖尿病PDL和BM分离的细胞显示矿化能力受损和成骨标志物下调。曲古霉素A或5-氮杂胞苷处理恢复矿化电位，增加ALP活性，上调RUNX2和β-catenin的表达。结论：我们的研究结果揭示了糖尿病干细胞成骨分化能力受损的潜在表观遗传机制。这些发现强调了表观遗传调节剂在恢复干细胞功能和增强骨再生方面的潜力。这种方法有望改善糖尿病相关的骨骼并发症和推进组织工程策略，包括开发基于支架的治疗方法，用于骨折修复、牙周再生和糖尿病患者的种植体整合。

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引用次数: 0

Genetic instability at the PTEN locus and altered miRNA-21 and miRNA-200a expression in gastric cancer patients in Poland 波兰胃癌患者PTEN位点遗传不稳定性及miRNA-21和miRNA-200a表达改变

IF 2.5 4区医学 Q3 MEDICINE, RESEARCH & EXPERIMENTAL

Advances in medical sciences

Pub Date : 2025-05-28 DOI: 10.1016/j.advms.2025.05.003

Magdalena Dzikowiec , Sandra Galant , Przemysław Lik , Monika Migdalska-Sęk , Dariusz Nejc , Janusz Piekarski , Alicja Majos , Ewa Brzeziańska-Lasota , Dorota Pastuszak-Lewandoska

Purpose

Gastric cancer is often diagnosed late, and is associated with poor long-term prognosis. The aim of the study was to look for non-invasive potential biomarkers involved in gastric carcinogenesis, with diagnostic or prognostic significance.

Material/methods

Gastric tissue samples, from three different regions of the stomach, including the primary tumor, macroscopically unchanged gastric tissues, as well as serum, were collected from patients diagnosed with gastric cancer. Serum samples were also obtained from a control group. The analyzed parameters were: expression levels of PTEN, miRNA-21 and miRNA-200a using qPCR method and the frequency of LOH/MSI at the PTEN locus using four microsatellite markers.

Results

The obtained results revealed significantly decreased expression of PTEN in gastric tumor tissue and statistically significant differences between the studied tissue samples from different stomach regions. PTEN expression in patients with LOH/MSI was decreased two-fold compared to patients without genetic instability, indicating a potential mechanism of gene silencing. Another mechanism of PTEN silencing could be due to miRNA activity: significant negative correlations were found between PTEN and the studied miRNAs expression levels. In serum, miRNA-21 expression was increased in the group of patients, while miRNA-200a expression was decreased, and the differences were statistically significant compared to controls. Receiver operating characteristic curve analysis for miRNA-200a revealed 92 % sensitivity and 77 % specificity.

Conclusions

The obtained results suggest that miRNA-21 and miRNA-200a could be considered as diagnostic biomarkers differentiating patients with gastric cancer from healthy individuals; however, it should be verified on a larger group of patients.

目的：胃癌往往诊断较晚，长期预后较差。该研究的目的是寻找与胃癌发生有关的具有诊断或预后意义的非侵入性潜在生物标志物。材料/方法：对诊断为胃癌的患者，分别从胃的三个不同区域收集胃组织样本，包括原发肿瘤、宏观未改变的胃组织和血清。另取对照组血清样本。分析参数为：qPCR法检测PTEN、miRNA-21和miRNA-200a的表达水平，4个微卫星标记检测PTEN位点LOH/MSI频率。结果：得到的结果显示PTEN在胃肿瘤组织中的表达明显降低，不同胃区组织样本间差异有统计学意义。与没有遗传不稳定的患者相比，LOH/MSI患者的PTEN表达减少了两倍，这表明基因沉默的潜在机制。PTEN沉默的另一个机制可能与miRNA活性有关：PTEN与所研究的miRNA表达水平呈显著负相关。血清中，患者组miRNA-21表达升高，miRNA-200a表达降低，与对照组比较差异有统计学意义。miRNA-200a的受试者工作特征曲线分析显示灵敏度为92%，特异性为77%。结论：miRNA-21和miRNA-200a可作为胃癌患者与健康人的诊断性生物标志物；然而，它应该在更大的患者群体中得到验证。

{"title":"Genetic instability at the PTEN locus and altered miRNA-21 and miRNA-200a expression in gastric cancer patients in Poland","authors":"Magdalena Dzikowiec , Sandra Galant , Przemysław Lik , Monika Migdalska-Sęk , Dariusz Nejc , Janusz Piekarski , Alicja Majos , Ewa Brzeziańska-Lasota , Dorota Pastuszak-Lewandoska","doi":"10.1016/j.advms.2025.05.003","DOIUrl":"10.1016/j.advms.2025.05.003","url":null,"abstract":"<div><h3>Purpose</h3><div>Gastric cancer is often diagnosed late, and is associated with poor long-term prognosis. The aim of the study was to look for non-invasive potential biomarkers involved in gastric carcinogenesis, with diagnostic or prognostic significance.</div></div><div><h3>Material/methods</h3><div>Gastric tissue samples, from three different regions of the stomach, including the primary tumor, macroscopically unchanged gastric tissues, as well as serum, were collected from patients diagnosed with gastric cancer. Serum samples were also obtained from a control group. The analyzed parameters were: expression levels of <em>PTEN</em>, miRNA-21 and miRNA-200a using qPCR method and the frequency of LOH/MSI at the <em>PTEN locus</em> using four microsatellite markers.</div></div><div><h3>Results</h3><div>The obtained results revealed significantly decreased expression of <em>PTEN</em> in gastric tumor tissue and statistically significant differences between the studied tissue samples from different stomach regions. <em>PTEN</em> expression in patients with LOH/MSI was decreased two-fold compared to patients without genetic instability, indicating a potential mechanism of gene silencing. Another mechanism of <em>PTEN</em> silencing could be due to miRNA activity: significant negative correlations were found between <em>PTEN</em> and the studied miRNAs expression levels. In serum, miRNA-21 expression was increased in the group of patients, while miRNA-200a expression was decreased, and the differences were statistically significant compared to controls. Receiver operating characteristic curve analysis for miRNA-200a revealed 92 % sensitivity and 77 % specificity.</div></div><div><h3>Conclusions</h3><div>The obtained results suggest that miRNA-21 and miRNA-200a could be considered as diagnostic biomarkers differentiating patients with gastric cancer from healthy individuals; however, it should be verified on a larger group of patients.</div></div>","PeriodicalId":7347,"journal":{"name":"Advances in medical sciences","volume":"70 2","pages":"Pages 248-254"},"PeriodicalIF":2.5,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144186205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Therapeutic potential of bone marrow-derived very small embryonic-like stem cells followed by thrombin-activated platelet-rich plasma for endometrial regeneration 骨髓来源的非常小的胚胎样干细胞与凝血酶激活的富血小板血浆在子宫内膜再生中的治疗潜力。

IF 2.5 4区医学 Q3 MEDICINE, RESEARCH & EXPERIMENTAL

Advances in medical sciences

Pub Date : 2025-05-24 DOI: 10.1016/j.advms.2025.05.002

Jeevitaa Kshersagar , Akshay A. Kawale , Leena R. Chaudhari , Mrunal N. Damle , Rakesh Kumar Sharma , Meghnad G. Joshi

Purpose

Endometrium, a dynamic tissue undergoing cyclic changes, plays a pivotal role in reproductive health. Disruptions in its structure and function can lead to infertility and pregnancy complications. Stem cell-based therapies, including very small embryonic-like stem cells (VSELS) and platelet-rich plasma (PRP), have shown promise in tissue regeneration.

Methods

We investigated the efficacy of bone marrow-derived VSELS combined with thrombin-activated PRP (aPRP) for endometrial regeneration in a murine model of disturbed endometrium (DE). Characterization of bone marrow very small embryonic like stem cells (BM VSELS) revealed pluripotency markers and negative expression for CD34, Tie-2, Thy, CD133, CD90, and delta-like protein (DLK).

Results

Transplantation of BM VSELS-aPRP resulted in their engraftment in the endometrium, with enhanced endometrial thickness, collagen reformation, and improved marker expression compared to controls. Immunohistochemical analysis demonstrated increased expression of α-SMA, CK-18, CK-19, E-Cad, Cla-1, CX-40, and ZO-1 in the transplant group. Pregnancy outcomes improved in the BM VSELS-aPRP group, with successful conception and delivery of healthy pups.

Conclusion

This study highlights the regenerative potential of BM VSELS-aPRP for endometrial repair and suggests a novel therapeutic approach for endometrial disorders and infertility.

目的：子宫内膜是一种周期性变化的动态组织，在生殖健康中起着关键作用。其结构和功能的破坏可导致不孕和妊娠并发症。基于干细胞的治疗，包括非常小的胚胎样干细胞（VSELS）和富血小板血浆（PRP），在组织再生方面显示出前景。方法：研究骨髓源性VSELS联合凝血酶激活PRP （aPRP）对小鼠子宫内膜紊乱（DE）模型子宫内膜再生的作用。骨髓非常小胚胎样干细胞（BM VSELS）的表征显示了多能性标记和CD34、Tie-2、Thy、CD133、CD90和δ样蛋白（DLK）的负表达。结果：BM VSELS-aPRP移植后，其在子宫内膜中植入，与对照组相比，子宫内膜厚度增加，胶原蛋白重组，标志物表达改善。免疫组化分析显示移植组α-SMA、CK-18、CK-19、E-Cad、Cla-1、CX-40、ZO-1表达增加。BM VSELS-aPRP组妊娠结局改善，成功受孕并产下健康幼崽。结论：本研究强调了BM VSELS-aPRP在子宫内膜修复中的再生潜力，为子宫内膜疾病和不孕症的治疗提供了新的途径。

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引用次数: 0

Meconium ferritin amounts and birth size of neonates: a pilot study 胎铁蛋白含量与新生儿出生尺寸的初步研究。

IF 2.5 4区医学 Q3 MEDICINE, RESEARCH & EXPERIMENTAL

Advances in medical sciences

Pub Date : 2025-05-09 DOI: 10.1016/j.advms.2025.05.001

Ewa Skarżyńska , Artur Jakimiuk , Tadeusz Issat , Krzysztof Krasuski , Barbara Lisowska-Myjak

Purpose

Ferritin amounts that accumulate in the meconium may provide new postnatal insights into intrauterine iron homeostasis and neonatal preparedness for the postnatal period. The most dynamic increases in fetal iron stores and fetal growth occur during the third trimester.

Materials and methods

This study involved 122 neonates born between 36 and 41 weeks of gestation, with birth weights from 2650 g to 4960 g and birth lengths ranging from 50 cm to 60 cm. Ferritin amounts per gram of meconium were determined via ELISA in the first meconium passed after birth.

Results

A significant week-by-week increase in the birth weight and length (p < 0.05) was accompanied by decreasing meconium ferritin amounts (p = 0.021) across the gestational age range of 36–41 weeks. There were negative correlations (p < 0.05) between the systematic decrease in meconium ferritin amounts and the gestational age across the same range (r = -0.18) and between ferritin amounts and the birth weight and length of newborns (r = -0.20 and r = -0.31). Neonates born at 36–37 weeks of gestation had lower birth weight and length, while their meconium ferritin amounts were nearly twice as high as in neonates born at 38–39 weeks or 40–41 weeks (p < 0.05).

Conclusions

Systematic decreases in meconium ferritin amounts from 36 to 41 weeks of gestation may suggest a gradual and gestational age-appropriate maturation of the mechanisms responsible for adaptation of the fetus to postnatal life. Determining a cut-off value for meconium ferritin amounts could aid in optimal management of newborns after birth.

目的：积累在胎便铁蛋白量可能提供新的产后见解宫内铁稳态和新生儿准备产后期。胎儿铁储存和胎儿生长最动态的增加发生在妊娠晚期。材料与方法：本研究纳入122例妊娠36 ~ 41周出生的新生儿，出生体重2650 ~ 4960 g，出生体长50 ~ 60 cm。在出生后通过的第一次胎中，通过ELISA测定每克胎中铁蛋白的含量。结论：胎铁蛋白含量在妊娠36 ~ 41周期间的系统性下降可能表明胎儿适应产后生活的机制逐渐成熟。确定胎粪铁蛋白量的临界值有助于新生儿出生后的最佳管理。

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引用次数: 0

Association of systemic immune–inflammation index with in-hospital mortality of cardiogenic shock patients supported with extracorporeal membrane oxygenation 体外膜氧合支持下心源性休克患者全身免疫炎症指数与住院死亡率的关系

IF 2.5 4区医学 Q3 MEDICINE, RESEARCH & EXPERIMENTAL

Advances in medical sciences

Pub Date : 2025-05-03 DOI: 10.1016/j.advms.2025.04.002

Shixing Li , Hao Wang , Jin Yu, Jingsong Xu, Yan Xu

Purpose

The systemic immune-inflammation index (SII) is a useful predictor in cardiovascular diseases. The purpose of this study was to investigate the association of SII with in–hospital mortality in patients with cardiogenic shock (CS) supported with extracorporeal membrane oxygenation (ECMO).

Patients and Methods

A total of 126 CS patients received ECMO implantation between January 2020 and December 2023. SII was calculated as follows: SII = neutrophil count × platelet count / lymphocyte count at admission. Participants were divided into high or low SII group based on the cut–off value of SII. In–hospital mortality was compared between the groups.

Results

The optimal SII cut–off value for predicting in–hospital mortality in CS patients supported with ECMO was 1735.9 (AUC 0.68, p = 0.001). In–hospital mortality was significantly higher in the high SII group compared to the low SII group (59.09 % vs. 21.67 %, p <0.001). The univariate and multivariate logistic regression analyses had shown that SII and left ventricular ejection fraction (LVEF) were identified as independent predictors of in–hospital mortality in CS patients supported with ECMO (OR: 1.001, 95 % CI: 1.000–1.002, p = 0.007 and OR: 0.881, 95 % CI: 0.803–0.966, p = 0.007, respectively). SII combined with LVEF offered a superior prognostic capability compared to SII alone (AUC 0.707, v. s. AUC 0.68).

Conclusion

We demonstrated that elevated admission SII was independently associated with in-hospital mortality in CS patients supported with ECMO. These findings highlight the potential role of SII as an indicator of mortality risk in this population.

目的：全身免疫炎症指数（SII）是心血管疾病的有效预测指标。本研究的目的是探讨SII与体外膜氧合（ECMO）支持的心源性休克（CS）患者住院死亡率的关系。患者和方法：2020年1月至2023年12月，共126例CS患者接受ECMO植入。SII计算公式如下：SII =入院时中性粒细胞计数×血小板计数/淋巴细胞计数。根据SII的临界值将参与者分为高SII组和低SII组。比较两组之间的住院死亡率。结果：预测经ECMO支持的CS患者住院死亡率的最佳SII临界值为1735.9 （AUC 0.68, p = 0.001）。高SII组的住院死亡率明显高于低SII组（59.09% vs. 21.67%, p）结论：我们证明了入院SII升高与ECMO支持下CS患者的住院死亡率独立相关。这些发现强调了SII作为该人群死亡风险指标的潜在作用。

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引用次数: 0

Collagen (rs3134646) and ARHGAP15 (rs4662344) genetic variants may predispose to colonic diverticulosis 胶原蛋白（rs3134646）和ARHGAP15 （rs4662344）基因变异可能易患结肠憩室病

IF 2.5 4区医学 Q3 MEDICINE, RESEARCH & EXPERIMENTAL

Advances in medical sciences

Pub Date : 2025-04-22 DOI: 10.1016/j.advms.2025.04.001

Piotr Nehring , Grzegorz Placha , Adam Przybyłkowski

Purpose

Colonic diverticulosis is a common condition in older adults in the entire world. Besides environmental factors, there are emerging data on underlying genetic predisposition to diverticula formation. The study aimed to identify genetic factors associated with colonic diverticulosis.

Patients and methods

The study involved 323 patients, 134 with colonic diverticulosis, and 189 healthy controls. In all study participants the following genetic variants were assessed using RT-PCR: rs1800587 in IL1, rs16944, and rs2853550 in IL1B, rs3134646, and rs6434304 in COL3A1, rs3771810, and rs3771863 in TACR1, rs4644560 in NK2R, rs4662344 in ARHGAP15, rs67153654 in FAM155A, and rs7848647 in TNFSF15.

Results

Both, the allele C of the COL3A1 (rs3134646) variant and allele T of the ARHGAP15 (rs4662344) variant, were shown to be associated with diverticulosis, compared to healthy controls in co-dominant and recessive models. Both of them were more frequently reported in patients with colonic diverticulosis, compared to healthy controls.

There were no allelic or genetic associations in patients with diverticulosis or diverticulitis compared to healthy controls, for the following genetic variants: IL1A (rs1800587), IL1B (rs16944, rs2853550), COL3A1 (rs6434304), TACR1 (rs3771810, rs3771863), NK2R (rs4644560), FAM155A (rs67153654) and TNFSF15 (rs7848647).

Conclusions

The allele C of COL3A1 (rs3134646) and allele T of the ARHGAP15 (rs4662344) may predispose to colonic diverticulosis. When viewed in the context of previous studies, these findings suggest that the development of colonic diverticula may be influenced by the involvement and degradation of the extracellular matrix.

目的：结肠憩室病是世界范围内老年人的常见病。除了环境因素外，关于憩室形成的潜在遗传易感性的数据也在不断涌现。该研究旨在确定与结肠憩室病相关的遗传因素。患者和方法本研究纳入323例患者，134例结肠憩室病，189例健康对照。在所有研究参与者中，使用RT-PCR评估以下遗传变异：IL1中的rs1800587、IL1B中的rs16944和rs2853550、COL3A1中的rs3134646和rs6434304、TACR1中的rs3771810和rs3771863、NK2R中的rs4644560、ARHGAP15中的rs4662344、FAM155A中的rs67153654和TNFSF15中的rs7848647。结果在共显性和隐性模型中，与健康对照相比，COL3A1 （rs3134646）变异的等位基因C和ARHGAP15 （rs4662344）变异的等位基因T都与憩室病有关。与健康对照组相比，这两种情况在结肠憩室病患者中更常见。与健康对照组相比，憩室病或憩室炎患者的以下遗传变异：IL1A （rs1800587）、IL1B （rs16944、rs2853550）、COL3A1 （rs6434304）、TACR1 （rs3771810、rs3771863）、NK2R （rs4644560）、FAM155A （rs67153654）和TNFSF15 （rs7848647）没有等位基因或遗传关联。结论COL3A1等位基因C （rs3134646）和ARHGAP15等位基因T （rs4662344）可能易患结肠憩室病。从以往的研究来看，这些发现表明结肠憩室的发育可能受到细胞外基质的受累和降解的影响。

{"title":"Collagen (rs3134646) and ARHGAP15 (rs4662344) genetic variants may predispose to colonic diverticulosis","authors":"Piotr Nehring , Grzegorz Placha , Adam Przybyłkowski","doi":"10.1016/j.advms.2025.04.001","DOIUrl":"10.1016/j.advms.2025.04.001","url":null,"abstract":"<div><h3>Purpose</h3><div>Colonic diverticulosis is a common condition in older adults in the entire world. Besides environmental factors, there are emerging data on underlying genetic predisposition to diverticula formation. The study aimed to identify genetic factors associated with colonic diverticulosis.</div></div><div><h3>Patients and methods</h3><div>The study involved 323 patients, 134 with colonic diverticulosis, and 189 healthy controls. In all study participants the following genetic variants were assessed using RT-PCR: rs1800587 in <em>IL1</em>, rs16944, and rs2853550 in <em>IL1B</em>, rs3134646, and rs6434304 in <em>COL3A1</em>, rs3771810, and rs3771863 in <em>TACR1</em>, rs4644560 in <em>NK2R</em>, rs4662344 in <em>ARHGAP15</em>, rs67153654 in <em>FAM155A</em>, and rs7848647 in <em>TNFSF15</em>.</div></div><div><h3>Results</h3><div>Both, the allele C of the <em>COL3A1</em> (rs3134646) variant and allele T of the <em>ARHGAP15</em> (rs4662344) variant, were shown to be associated with diverticulosis, compared to healthy controls in co-dominant and recessive models. Both of them were more frequently reported in patients with colonic diverticulosis, compared to healthy controls.</div><div>There were no allelic or genetic associations in patients with diverticulosis or diverticulitis compared to healthy controls, for the following genetic variants: <em>IL1A</em> (rs1800587), <em>IL1B</em> (rs16944, rs2853550), <em>COL3A1</em> (rs6434304), <em>TACR1</em> (rs3771810, rs3771863), <em>NK2R</em> (rs4644560), <em>FAM155A</em> (rs67153654) and <em>TNFSF15</em> (rs7848647).</div></div><div><h3>Conclusions</h3><div>The allele C of <em>COL3A1</em> (rs3134646) and allele T of the <em>ARHGAP15</em> (rs4662344) may predispose to colonic diverticulosis. When viewed in the context of previous studies, these findings suggest that the development of colonic diverticula may be influenced by the involvement and degradation of the extracellular matrix.</div></div>","PeriodicalId":7347,"journal":{"name":"Advances in medical sciences","volume":"70 2","pages":"Pages 231-236"},"PeriodicalIF":2.5,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143907093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of salusin-β in paediatric patients with chronic kidney disease or hypertension 慢性肾病或高血压患儿salusin-β的评价

IF 2.5 4区医学 Q3 MEDICINE, RESEARCH & EXPERIMENTAL

Advances in medical sciences

Pub Date : 2025-03-01 DOI: 10.1016/j.advms.2025.02.006

Mirjam Močnik , Sonja Golob Jančič , Martina Filipič , Evgenija Homšak , Mateja Svetej , Nataša Marčun Varda

Purpose

Salusins are newly identified endogenous peptides implicated in the atherosclerotic process. Salusin-β, in particular, is recognized for its proatherogenic role. Given that atherosclerosis can commence in childhood, salusin-β holds promise as a potential biomarker for cardiovascular risk assessment. The objective of our study was to investigate salusin-β levels in children with early stages of chronic kidney disease (CKD) or hypertension (HTN), and compare them to healthy controls. Furthermore, we aimed to evaluate its association with obesity and pulse wave velocity (PWV), the latter being a well-established marker for determining arterial elasticity.

Materials and methods

This cross-sectional study involved 96 paediatric patients, including 46 with CKD and 50 with HTN, as well as 33 healthy controls. Anthropometric measurements, PWV assessments, serum salusin-β values, and basic laboratory investigations were conducted for all participants.

Results

Salusin-β levels were found to be elevated in patients with CKD (p = 0.014), but not in patients with HTN when compared to healthy controls. When correlating salusin-β levels with PWV, a significant but weak correlation was observed (r = 0.211, p = 0.020).

Conclusions

Salusin-β levels were elevated in paediatric patients with CKD. Additionally, salusin-β levels correlated significantly with PWV. Obesity played a smaller role in these correlations, with significant correlations observed only after combining cardiovascular risk factors revealing certain associations between salusin-β levels and some cardiovascular variables, but with inconclusive findings and, in some instances, even contrary to anticipated outcomes.

目的alusins是新发现的与动脉粥样硬化过程有关的内源性肽。特别是Salusin-β，被认为具有促进动脉粥样硬化的作用。鉴于动脉粥样硬化可以在儿童时期开始，salusin-β有望成为心血管风险评估的潜在生物标志物。本研究的目的是调查早期慢性肾脏疾病（CKD）或高血压（HTN）儿童的salusin-β水平，并将其与健康对照进行比较。此外，我们旨在评估其与肥胖和脉搏波速度（PWV）的关系，后者是确定动脉弹性的公认标志。材料和方法本横断面研究纳入96例儿科患者，包括46例CKD患者和50例HTN患者，以及33例健康对照。对所有参与者进行了人体测量、PWV评估、血清salusin-β值和基础实验室调查。结果与健康对照相比，CKD患者的salusin -β水平升高（p = 0.014），而HTN患者的salusin -β水平没有升高。当salusin-β水平与PWV相关时，观察到显著但微弱的相关性（r = 0.211, p = 0.020）。结论儿童CKD患者salusin -β水平升高。此外，salusin-β水平与PWV显著相关。肥胖在这些相关性中所起的作用较小，只有在结合心血管危险因素揭示salusin-β水平与一些心血管变量之间的某些关联后，才能观察到显著的相关性，但结果不确定，在某些情况下，甚至与预期结果相反。

{"title":"Evaluation of salusin-β in paediatric patients with chronic kidney disease or hypertension","authors":"Mirjam Močnik , Sonja Golob Jančič , Martina Filipič , Evgenija Homšak , Mateja Svetej , Nataša Marčun Varda","doi":"10.1016/j.advms.2025.02.006","DOIUrl":"10.1016/j.advms.2025.02.006","url":null,"abstract":"<div><h3>Purpose</h3><div>Salusins are newly identified endogenous peptides implicated in the atherosclerotic process. Salusin-β, in particular, is recognized for its proatherogenic role. Given that atherosclerosis can commence in childhood, salusin-β holds promise as a potential biomarker for cardiovascular risk assessment. The objective of our study was to investigate salusin-β levels in children with early stages of chronic kidney disease (CKD) or hypertension (HTN), and compare them to healthy controls. Furthermore, we aimed to evaluate its association with obesity and pulse wave velocity (PWV), the latter being a well-established marker for determining arterial elasticity.</div></div><div><h3>Materials and methods</h3><div>This cross-sectional study involved 96 paediatric patients, including 46 with CKD and 50 with HTN, as well as 33 healthy controls. Anthropometric measurements, PWV assessments, serum salusin-β values, and basic laboratory investigations were conducted for all participants.</div></div><div><h3>Results</h3><div>Salusin-β levels were found to be elevated in patients with CKD (<em>p</em> = 0.014), but not in patients with HTN when compared to healthy controls. When correlating salusin-β levels with PWV, a significant but weak correlation was observed (r = 0.211, <em>p</em> = 0.020).</div></div><div><h3>Conclusions</h3><div>Salusin-β levels were elevated in paediatric patients with CKD. Additionally, salusin-β levels correlated significantly with PWV. Obesity played a smaller role in these correlations, with significant correlations observed only after combining cardiovascular risk factors revealing certain associations between salusin-β levels and some cardiovascular variables, but with inconclusive findings and, in some instances, even contrary to anticipated outcomes.</div></div>","PeriodicalId":7347,"journal":{"name":"Advances in medical sciences","volume":"70 1","pages":"Pages 184-190"},"PeriodicalIF":2.5,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143527227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0