Anita Birinji, Dušan Lalošević, Željko Mihaljev, Kristina Pogrmić-Majkić, Anita Radovanović, Radmila Glišić, Marija Marin
This study aimed to measure the total arsenic content deposited in the ovaries of three consecutive generations of mice. The animals were treated with two different concentrations to determine whether histological changes were caused in the ovaries. The control group of mice received tap water, whereas the experimental groups were given different concentrations of dissolved arsenic (III)-oxide. The arsenic content in the ovaries in both experimental groups increased with each generation. The highest content was recorded in the third generation of the second experimental group. Between the two experimental groups in each generation, significant differences in the average number of corpora lutea and ovarian follicles were identified. Arsenic caused structural changes in the ovaries in both experimental groups in all three consecutive generations.
{"title":"Impact of Arsenic on Mouse Ovaries Over Three Generations","authors":"Anita Birinji, Dušan Lalošević, Željko Mihaljev, Kristina Pogrmić-Majkić, Anita Radovanović, Radmila Glišić, Marija Marin","doi":"10.2478/acve-2024-0005","DOIUrl":"https://doi.org/10.2478/acve-2024-0005","url":null,"abstract":"This study aimed to measure the total arsenic content deposited in the ovaries of three consecutive generations of mice. The animals were treated with two different concentrations to determine whether histological changes were caused in the ovaries. The control group of mice received tap water, whereas the experimental groups were given different concentrations of dissolved arsenic (III)-oxide. The arsenic content in the ovaries in both experimental groups increased with each generation. The highest content was recorded in the third generation of the second experimental group. Between the two experimental groups in each generation, significant differences in the average number of <jats:italic>corpora lutea</jats:italic> and ovarian follicles were identified. Arsenic caused structural changes in the ovaries in both experimental groups in all three consecutive generations.","PeriodicalId":7379,"journal":{"name":"Acta Veterinaria","volume":"155 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140154853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Isidora Prošić, Natalija Milčić-Matić, Nenad Milić, Andrea Radalj, Ksenija Aksentijević, Milica Ilić, Jakov Nišavić, Marina Radojičić, Vladimir Gajdov, Dejan Krnjaić
The escalating global concern of antimicrobial resistance in human and veterinary medicine is exacerbated by the inappropriate prescription of antibiotics for bacterial infections in companion animals. This study aimed to determine the distribution of coagulase-positive staphylococci causing clinical skin and ear infections in dogs and to determine methicillin-resistant isolates. A total of 78 staphylococcal strains were isolated from clinical samples taken from patients at the Dermatology Clinic at the Faculty of Veterinary Medicine in Belgrade, Serbia. Multiplex PCR was used for species-specific identification, and mecA and mecC genes were used to determine methicillin resistance, in addition to phenotypic determination, MIC values and detection of PBP2a. Out of the 78 samples analyzed, 65.8% were identified as Staphylococcus pseudintermedius, 22.4% as S. aureus, 7.9% as S. coagulans, and 3.9% as S. intermedius. Four S. aureus isolates exhibited methicillin resistance confirmed by cefoxitin disk diffusion, while five were confirmed with MIC testing and latex agglutination. MecA gene was detected in 29.4% of S. aureus and 30% of S. pseudintermedius isolates. These isolates were classified as methicillin-resistant S. aureus (MRSA) and methicillin-resistant S. pseudintermedius (MRSP), respectively. No isolates carried the mecC gene. This study provides insights into the prevalence of CoPS species and methicillin resistance in isolates from dogs. Continued surveillance is essential to monitor and understand the emergence and dissemination of antimicrobial resistance in veterinary medicine and the results of this study accent the need for establishment of a continuous antimicrobial resistance surveillance program in the Republic of Serbia.
{"title":"Molecular Prevalence of MecA and MecC Genеs in Coagulasе-Positive Staphylococci Isolated From Dogs with Dermatitis and Otitis in Belgrade, Serbia: A One Year Study","authors":"Isidora Prošić, Natalija Milčić-Matić, Nenad Milić, Andrea Radalj, Ksenija Aksentijević, Milica Ilić, Jakov Nišavić, Marina Radojičić, Vladimir Gajdov, Dejan Krnjaić","doi":"10.2478/acve-2024-0009","DOIUrl":"https://doi.org/10.2478/acve-2024-0009","url":null,"abstract":"The escalating global concern of antimicrobial resistance in human and veterinary medicine is exacerbated by the inappropriate prescription of antibiotics for bacterial infections in companion animals. This study aimed to determine the distribution of coagulase-positive staphylococci causing clinical skin and ear infections in dogs and to determine methicillin-resistant isolates. A total of 78 staphylococcal strains were isolated from clinical samples taken from patients at the Dermatology Clinic at the Faculty of Veterinary Medicine in Belgrade, Serbia. Multiplex PCR was used for species-specific identification, and <jats:italic>mecA</jats:italic> and <jats:italic>mecC</jats:italic> genes were used to determine methicillin resistance, in addition to phenotypic determination, MIC values and detection of PBP2a. Out of the 78 samples analyzed, 65.8% were identified as <jats:italic>Staphylococcus pseudintermedius</jats:italic>, 22.4% as <jats:italic>S. aureus</jats:italic>, 7.9% as <jats:italic>S. coagulans</jats:italic>, and 3.9% as <jats:italic>S. intermedius</jats:italic>. Four <jats:italic>S. aureus</jats:italic> isolates exhibited methicillin resistance confirmed by cefoxitin disk diffusion, while five were confirmed with MIC testing and latex agglutination. <jats:italic>MecA</jats:italic> gene was detected in 29.4% of <jats:italic>S. aureus</jats:italic> and 30% of <jats:italic>S. pseudintermedius</jats:italic> isolates. These isolates were classified as methicillin-resistant <jats:italic>S. aureus</jats:italic> (MRSA) and methicillin-resistant <jats:italic>S. pseudintermedius</jats:italic> (MRSP), respectively. No isolates carried the <jats:italic>mecC</jats:italic> gene. This study provides insights into the prevalence of CoPS species and methicillin resistance in isolates from dogs. Continued surveillance is essential to monitor and understand the emergence and dissemination of antimicrobial resistance in veterinary medicine and the results of this study accent the need for establishment of a continuous antimicrobial resistance surveillance program in the Republic of Serbia.","PeriodicalId":7379,"journal":{"name":"Acta Veterinaria","volume":"55 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140155269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Brucella ovis infects sheep and causes a clinical or subclinical disease characterized by genital lesions and reduced fertility in rams, placentitis and abortions in ewes, and increased perinatal mortality in lambs. As part of this study, analyses were conducted on a sheep farm near Belgrade (Serbia). Of the serological tests, indirect ELISA was performed. A total of 94 blood sera were analyzed, 33 from rams and 61 from ewes. The results showed 23 (69.7%) positive findings in rams and 2 (3.3%) positive findings in ewes, with an overall prevalence of 25.4% bounded by a 95% confidence interval. Bruce-ladder multiplex PCR and Real time PCR were performed on 19 serologically positive rams and two serologically positive ewes. The results indicate a slightly higher sensitivity of Real time PCR compared to conventional PCR in diagnosing B. ovis from the reproductive tissues of rams. However, the differences in sensitivity between different nucleic acid extraction protocols were not significant. Most rams showed a positive PCR result in only one sample of reproductive tissue, suggesting the recommendation to take multiple samples from each animal. Further research is needed to bring the sensitivity of molecular tests in diagnosing ram epididymitis closer to the sensitivity of serological tests.
{"title":"Reliability of Molecular Tests in Diagnosing Ovine Brucellosis Caused by Brucella Ovis","authors":"Mladen Zelenović, Darko Marinković, Nataša Stević, Slavoljub Stanojević, Milan Aničić, Vesna Milićević, Olivera Valčić, Sonja Radojičić","doi":"10.2478/acve-2024-0010","DOIUrl":"https://doi.org/10.2478/acve-2024-0010","url":null,"abstract":"<jats:italic>Brucella ovis</jats:italic> infects sheep and causes a clinical or subclinical disease characterized by genital lesions and reduced fertility in rams, placentitis and abortions in ewes, and increased perinatal mortality in lambs. As part of this study, analyses were conducted on a sheep farm near Belgrade (Serbia). Of the serological tests, indirect ELISA was performed. A total of 94 blood sera were analyzed, 33 from rams and 61 from ewes. The results showed 23 (69.7%) positive findings in rams and 2 (3.3%) positive findings in ewes, with an overall prevalence of 25.4% bounded by a 95% confidence interval. Bruce-ladder multiplex PCR and Real time PCR were performed on 19 serologically positive rams and two serologically positive ewes. The results indicate a slightly higher sensitivity of Real time PCR compared to conventional PCR in diagnosing <jats:italic>B. ovis</jats:italic> from the reproductive tissues of rams. However, the differences in sensitivity between different nucleic acid extraction protocols were not significant. Most rams showed a positive PCR result in only one sample of reproductive tissue, suggesting the recommendation to take multiple samples from each animal. Further research is needed to bring the sensitivity of molecular tests in diagnosing ram epididymitis closer to the sensitivity of serological tests.","PeriodicalId":7379,"journal":{"name":"Acta Veterinaria","volume":"5 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140154999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The utilization of stored platelet transfusion has emerged as an effective approach in the management of thrombocytopenia. However, the limited availability of fresh platelets in veterinary medicine due to the challenging storage requirements poses a significant constraint. Lyophilized platelets offer extended shelf-life and convenient storage options, enhancing their accessibility for thrombocytopenic patients. However, only a limited number of studies have examined the clearance and survival rate of transfused lyophilized platelets, particularly regarding the lyophilization technique utilizing trehalose as a platelet stabilization agent. The objective of this study was to assess the recovery and survival rate of trehalose lyophilized platelets within the circulatory system. To investigate these parameters, CMFDA-labeled lyophilized platelets were administered to mice, and their recovery and survival rates were analyzed. Flow cytometric analysis revealed the rapid clearance of lyophilized platelets from the systemic circulation. The immediate post-infusion percent recovery of labeled platelet particles was 42.7 ± 8.15 %. The average survival rates at post-infusion time points at 15, 30, 45, and 60 minutes were 28.2 ± 4.31, 14.5 ± 3.56, 5.1 ± 2.02, and 0.82 ± 0.57, respectively. The calculated mean half-life was 8.39 ± 0.44 minutes. The most pronounced decrease in labeled lyophilized platelet count occurred during the 30-minute timeframe immediately following infusion. Subsequently, over 99% of lyophilized platelets were eliminated after 60 minutes post-infusion. These findings indicate that higher dosages and more frequent administration of trehalose lyophilized platelets might be necessary to achieve a therapeutic effect comparable to that of fresh platelets.
{"title":"Clearance of Trehalose Lyophilized Platelets in Mice","authors":"Mu-Young Kim, Hyun-Jung Han","doi":"10.2478/acve-2023-0039","DOIUrl":"https://doi.org/10.2478/acve-2023-0039","url":null,"abstract":"The utilization of stored platelet transfusion has emerged as an effective approach in the management of thrombocytopenia. However, the limited availability of fresh platelets in veterinary medicine due to the challenging storage requirements poses a significant constraint. Lyophilized platelets offer extended shelf-life and convenient storage options, enhancing their accessibility for thrombocytopenic patients. However, only a limited number of studies have examined the clearance and survival rate of transfused lyophilized platelets, particularly regarding the lyophilization technique utilizing trehalose as a platelet stabilization agent. The objective of this study was to assess the recovery and survival rate of trehalose lyophilized platelets within the circulatory system. To investigate these parameters, CMFDA-labeled lyophilized platelets were administered to mice, and their recovery and survival rates were analyzed. Flow cytometric analysis revealed the rapid clearance of lyophilized platelets from the systemic circulation. The immediate post-infusion percent recovery of labeled platelet particles was 42.7 ± 8.15 %. The average survival rates at post-infusion time points at 15, 30, 45, and 60 minutes were 28.2 ± 4.31, 14.5 ± 3.56, 5.1 ± 2.02, and 0.82 ± 0.57, respectively. The calculated mean half-life was 8.39 ± 0.44 minutes. The most pronounced decrease in labeled lyophilized platelet count occurred during the 30-minute timeframe immediately following infusion. Subsequently, over 99% of lyophilized platelets were eliminated after 60 minutes post-infusion. These findings indicate that higher dosages and more frequent administration of trehalose lyophilized platelets might be necessary to achieve a therapeutic effect comparable to that of fresh platelets.","PeriodicalId":7379,"journal":{"name":"Acta Veterinaria","volume":"20 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138682793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Andrea Pereira da Costa, Thaliane França Costa, Suellen de Araújo Barbosa, Alcyjara Rêgo Costa, Ana Lúcia Abreu-Silva, Fábio Henrique Evangelista Andrade, Isabel Silva Oliveira, Rita de Maria Seabra Nogueira
An 11-year-old male dog presenting exophthalmia and perforation of the cornea of the left eye was seen at the veterinary hospital of the State University of Maranhão. Upon physical examination, irregularly shaped granulomas of crumbly appearance were observed on the dog’s tongue. The dog was sent for ocular enucleation surgery. The following tests were requested: complete blood count, serum biochemistry, hemoparasite investigation, chest X-Ray, echocardiogram, electrocardiogram, cytology and nodule histopathology. The blood count revealed neutrophilia, signs of anemia, increased total plasma protein and thrombocytopenia. Serum biochemistry and the imaging examinations showed normal results. The cytological examination on the tongue nodules showed microfilariae and the histopathological examination showed chronic glossitis. Polymerase chain reaction (PCR) and genetic sequencing were performed on a blood sample, which made it possible to identify the species Acanthocheilonema reconditum. This is the first record worldwide of the occurrence of microfilariae of A. reconditum on tongue nodules. This finding serves to alert dermatologists and dentists regarding the need for a differential diagnosis for types of oral cavity lesions of unknown etiological origin.
马拉尼昂州立大学兽医院接诊了一只 11 岁的雄性犬，它患有眼球外翻和左眼角膜穿孔。经体格检查，发现该犬的舌头上有形状不规则的肉芽肿，呈碎屑状。该犬被送去进行眼球摘除手术。医生要求对该犬进行以下检查：全血细胞计数、血清生化检查、血液寄生虫检查、胸部 X 光检查、超声心动图检查、心电图检查、细胞学检查和结节组织病理学检查。血细胞计数显示中性粒细胞增多、贫血迹象、血浆总蛋白增加和血小板减少。血清生化和影像学检查结果显示正常。舌结节的细胞学检查显示有微丝蚴，组织病理学检查显示有慢性舌炎。对血液样本进行了聚合酶链式反应（PCR）和基因测序，从而确定了 Acanthocheilonema reconditum 这一物种。这是世界上首次在舌结节上发现 A. reconditum 的微丝蚴。这一发现提醒皮肤科医生和牙科医生需要对病因不明的口腔病变类型进行鉴别诊断。
{"title":"Acanthocheilonema Reconditum (Spirurida: Onchocercidae) in the Oral Cavity of a Dog in Northeastern Brazil: Case Report","authors":"Andrea Pereira da Costa, Thaliane França Costa, Suellen de Araújo Barbosa, Alcyjara Rêgo Costa, Ana Lúcia Abreu-Silva, Fábio Henrique Evangelista Andrade, Isabel Silva Oliveira, Rita de Maria Seabra Nogueira","doi":"10.2478/acve-2023-0040","DOIUrl":"https://doi.org/10.2478/acve-2023-0040","url":null,"abstract":"An 11-year-old male dog presenting exophthalmia and perforation of the cornea of the left eye was seen at the veterinary hospital of the State University of Maranhão. Upon physical examination, irregularly shaped granulomas of crumbly appearance were observed on the dog’s tongue. The dog was sent for ocular enucleation surgery. The following tests were requested: complete blood count, serum biochemistry, hemoparasite investigation, chest X-Ray, echocardiogram, electrocardiogram, cytology and nodule histopathology. The blood count revealed neutrophilia, signs of anemia, increased total plasma protein and thrombocytopenia. Serum biochemistry and the imaging examinations showed normal results. The cytological examination on the tongue nodules showed microfilariae and the histopathological examination showed chronic glossitis. Polymerase chain reaction (PCR) and genetic sequencing were performed on a blood sample, which made it possible to identify the species <jats:italic>Acanthocheilonema reconditum.</jats:italic> This is the first record worldwide of the occurrence of microfilariae of <jats:italic>A. reconditum</jats:italic> on tongue nodules. This finding serves to alert dermatologists and dentists regarding the need for a differential diagnosis for types of oral cavity lesions of unknown etiological origin.","PeriodicalId":7379,"journal":{"name":"Acta Veterinaria","volume":"68 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138682794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Various endogenous and exogenous factors influence the occurrence of oxidative stress in all organisms, as well as in dogs. An increase in reactive oxygen species (ROS) concentration and the occurrence of oxidative stress can lead to changes in the structure of proteins, lipids, and DNA. The level of oxidative stress can be determined by measuring the end products of lipid peroxidation known as reactive substances of thiobarbituric acid (TBARS) of which malondialdehyde (MDA) is the most important. The concentration of MDA can be easily measured in various tissues and body excretions, but also by a non-invasive method of hair sampling. In this research, we have collected dog hair in grooming saloons, fluorometrically measured TBARS levels and compared the obtained values with factors such as breed, sex, age, passive smoking, sterilization, and season. No significant difference between sterilized and non-sterilized dogs was observed. The intensity of lipid peroxidation differed between the sexes, dog breeds, status of smoking by owner and exposure to UV radiation.
各种内源性和外源性因素都会影响氧化应激在所有生物体和狗体内的发生。活性氧（ROS）浓度的增加和氧化应激的发生会导致蛋白质、脂质和 DNA 结构的改变。氧化应激的程度可以通过测量脂质过氧化的最终产物--硫代巴比妥酸活性物质（TBARS）来确定，其中丙二醛（MDA）是最重要的物质。MDA 的浓度很容易在各种组织和身体排泄物中测量，也可以通过毛发取样这种非侵入性方法测量。在这项研究中，我们在美容院采集了狗的毛发，用荧光计测量了 TBARS 水平，并将所得数值与品种、性别、年龄、被动吸烟、绝育和季节等因素进行了比较。结果表明，绝育犬和未绝育犬之间没有明显差异。脂质过氧化的强度在性别、狗的品种、主人吸烟情况和暴露于紫外线辐射下的情况之间存在差异。
{"title":"Thiobarbituric Acid Reactive Substances (TBARS) in Dog’s Hair as a Sign of Oxidative Stress – Preliminary Study","authors":"Nikolina Boić, Branimir Hackenberger Kutuzović, Davorka Hackenberger Kutuzović","doi":"10.2478/acve-2023-0034","DOIUrl":"https://doi.org/10.2478/acve-2023-0034","url":null,"abstract":"Various endogenous and exogenous factors influence the occurrence of oxidative stress in all organisms, as well as in dogs. An increase in reactive oxygen species (ROS) concentration and the occurrence of oxidative stress can lead to changes in the structure of proteins, lipids, and DNA. The level of oxidative stress can be determined by measuring the end products of lipid peroxidation known as reactive substances of thiobarbituric acid (TBARS) of which malondialdehyde (MDA) is the most important. The concentration of MDA can be easily measured in various tissues and body excretions, but also by a non-invasive method of hair sampling. In this research, we have collected dog hair in grooming saloons, fluorometrically measured TBARS levels and compared the obtained values with factors such as breed, sex, age, passive smoking, sterilization, and season. No significant difference between sterilized and non-sterilized dogs was observed. The intensity of lipid peroxidation differed between the sexes, dog breeds, status of smoking by owner and exposure to UV radiation.","PeriodicalId":7379,"journal":{"name":"Acta Veterinaria","volume":"52 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138682789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Linden honey represents a unique honey variety valued for its nutritional benefits, distinctive taste and aroma. Phenols, polyphenols, flavonoids, ascorbic acid and phenolic acids in honey have antioxidant activities. This study aimed to investigate the effects of linden honey on the activities of antioxidant enzymes catalase (CAT) and glutathione peroxidase (GPx), as well as on the concentration of malondialdehyde (MDA) in individually housed animals. The investigated parameters were quantified using spectrophotometric method for determination of enzyme activities and MDA concentration in the blood. We found that treatment with linden honey in the socially isolated animals significantly increased the enzyme activities of CAT and GPx, and significantly decreased the concentration of MDA. The modulation of CAT and GPx activities in socially isolated animals treated with linden honey may be very important for understanding the role of honey in the capacity of antioxidant defense system to increase and maintain its stability in psychosocial stress conditions. Our results may be important in biomedical research for understanding the role of honey in the amelioration of oxidative stress.
{"title":"Antioxidant Status in the Blood of Psychosocially Stressed Rats Treated with Honey","authors":"Ljubica Gavrilović, Vesna Stojiljković, Vojislav Stanić, Dragoljub Jovanović, Snežana Pejić, Branka Borović, Snežana B. Pajović","doi":"10.2478/acve-2023-0035","DOIUrl":"https://doi.org/10.2478/acve-2023-0035","url":null,"abstract":"Linden honey represents a unique honey variety valued for its nutritional benefits, distinctive taste and aroma. Phenols, polyphenols, flavonoids, ascorbic acid and phenolic acids in honey have antioxidant activities. This study aimed to investigate the effects of linden honey on the activities of antioxidant enzymes catalase (CAT) and glutathione peroxidase (GPx), as well as on the concentration of malondialdehyde (MDA) in individually housed animals. The investigated parameters were quantified using spectrophotometric method for determination of enzyme activities and MDA concentration in the blood. We found that treatment with linden honey in the socially isolated animals significantly increased the enzyme activities of CAT and GPx, and significantly decreased the concentration of MDA. The modulation of CAT and GPx activities in socially isolated animals treated with linden honey may be very important for understanding the role of honey in the capacity of antioxidant defense system to increase and maintain its stability in psychosocial stress conditions. Our results may be important in biomedical research for understanding the role of honey in the amelioration of oxidative stress.","PeriodicalId":7379,"journal":{"name":"Acta Veterinaria","volume":"12 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138682588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Csilla Tóthová, Alexandra Valenčáková, Ľubica Horňáková, Oskar Nagy
Alterations in the serum protein pattern may be associated with many diseases, including neoplastic processes. In veterinary medicine, these changes are poorly understood. Therefore, this study was aimed at the analysis of the distribution of blood serum protein fractions separated by agarose gel electrophoresis, and at the determination of the concentrations of main acute phase proteins in bitches with mammary gland neoplasia. The evaluation was conducted on twelve female dogs with palpable single or multiple nodules in the parenchyma of the mammary gland and on ten tumor-free clinically healthy bitches to compare the possible differences in the obtained results. Blood serum was used to perform agarose gel electrophoresis of the main blood serum protein fractions and to analyze the concentrations of total serum proteins and the following canine acute phase proteins: serum amyloid A, haptoglobin, C-reactive protein and α1-acid glycoprotein. The concentrations of total serum proteins were slightly higher in bitches with mammary gland tumors. Serum protein electrophoresis showed lower mean concentrations of albumin and α1-globulins in the affected dogs, while the concentrations of α2-and β1-globulins were significantly higher (P=0.0032 and P=0.0021, respectively) compared to dogs without mammary gland tumors. In the concentrations of acute phase proteins, significantly higher mean concentrations of C-reactive protein and haptoglobin were obtained in dogs with mammary tumors (P=0.0025 and P=0.0002, respectively). The values of α1-acid glycoprotein did not vary markedly between the bitches with and without mammary tumors. Presented data suggest that neoplastic processes in the mammary gland may also alter the electrophoretic pattern of blood serum proteins and induce changes in the production of some inflammatory proteins.
{"title":"Comparison of the Serum Protein Electrophoretic Pattern and Concentrations of Acute Phase Proteins in Bitches with and Without Mammary Gland Tumors","authors":"Csilla Tóthová, Alexandra Valenčáková, Ľubica Horňáková, Oskar Nagy","doi":"10.2478/acve-2023-0037","DOIUrl":"https://doi.org/10.2478/acve-2023-0037","url":null,"abstract":"Alterations in the serum protein pattern may be associated with many diseases, including neoplastic processes. In veterinary medicine, these changes are poorly understood. Therefore, this study was aimed at the analysis of the distribution of blood serum protein fractions separated by agarose gel electrophoresis, and at the determination of the concentrations of main acute phase proteins in bitches with mammary gland neoplasia. The evaluation was conducted on twelve female dogs with palpable single or multiple nodules in the parenchyma of the mammary gland and on ten tumor-free clinically healthy bitches to compare the possible differences in the obtained results. Blood serum was used to perform agarose gel electrophoresis of the main blood serum protein fractions and to analyze the concentrations of total serum proteins and the following canine acute phase proteins: serum amyloid A, haptoglobin, C-reactive protein and α<jats:sub>1</jats:sub>-acid glycoprotein. The concentrations of total serum proteins were slightly higher in bitches with mammary gland tumors. Serum protein electrophoresis showed lower mean concentrations of albumin and α<jats:sub>1</jats:sub>-globulins in the affected dogs, while the concentrations of α<jats:sub>2</jats:sub>-and β<jats:sub>1</jats:sub>-globulins were significantly higher (P=0.0032 and P=0.0021, respectively) compared to dogs without mammary gland tumors. In the concentrations of acute phase proteins, significantly higher mean concentrations of C-reactive protein and haptoglobin were obtained in dogs with mammary tumors (P=0.0025 and P=0.0002, respectively). The values of α<jats:sub>1</jats:sub>-acid glycoprotein did not vary markedly between the bitches with and without mammary tumors. Presented data suggest that neoplastic processes in the mammary gland may also alter the electrophoretic pattern of blood serum proteins and induce changes in the production of some inflammatory proteins.","PeriodicalId":7379,"journal":{"name":"Acta Veterinaria","volume":"26 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138682589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Alexandra Valencakova, Beata Kiselova-Bilekova, Maria Figurova, Slavomir Hornak, Lubica Hornakova
The aim of this study was to determine and compare values of carcinoembryonic antigen (CEA) and cancer antigen (CA 15-3) in 50 bitches with mammary tumors and 150 clinically healthy dogs. A modified procedure was used to determine the CEA and CA 15-3 markers with the human kits using the radioimmunoassay method (RIA). Samples collected from extirpated tumors of mammary glands were histologically processed and classified as per WHO guidelines. The mean values of the carcinoembryonic antigen markers ± SD were as follows: control group 0.89 ± 0.79, group with mammary gland tumor 1.53 ± 1.15. The values of cancer antigen markers CA 15-3 ± SD were: 1.52 ± 0.66 and 2.87 ± 1.11, respectively. The statistical significance for the carcinoembryonic antigen marker between groups was P < 0.0001. The cancer antigen CA 15-3 values between groups were also statistically significant with P < 0.0001. The results of the present study show that there are significant differences in both antigens between the control group and groups with mammary gland tumor in dogs.
本研究的目的是测定并比较 50 只患有乳腺肿瘤的母犬和 150 只临床健康犬的癌胚抗原 (CEA) 和癌抗原 (CA 15-3) 值。使用改良程序，采用放射免疫分析法（RIA）用人用试剂盒测定癌胚抗原（CEA）和癌抗原（CA 15-3）标记物。从切除的乳腺肿瘤中采集的样本经组织学处理后，按照世界卫生组织的指南进行分类。癌胚抗原标记物的平均值（± SD）如下：对照组 0.89 ± 0.79，乳腺肿瘤组 1.53 ± 1.15。癌症抗原标记物 CA 15-3 的值（± SD）为：对照组 0.89 ± 0.79，乳腺肿瘤组 1.53 ± 1.15：分别为（1.52 ± 0.66）和（2.87 ± 1.11）。组间癌胚抗原标志物的统计学意义为 P < 0.0001。组间癌抗原 CA 15-3 值的统计学意义也是 P < 0.0001。本研究结果表明，对照组和患乳腺肿瘤组之间的两种抗原均存在显著差异。
{"title":"Tumor Markers in Dogs with Mammary Gland Tumors","authors":"Alexandra Valencakova, Beata Kiselova-Bilekova, Maria Figurova, Slavomir Hornak, Lubica Hornakova","doi":"10.2478/acve-2023-0036","DOIUrl":"https://doi.org/10.2478/acve-2023-0036","url":null,"abstract":"The aim of this study was to determine and compare values of carcinoembryonic antigen (CEA) and cancer antigen (CA 15-3) in 50 bitches with mammary tumors and 150 clinically healthy dogs. A modified procedure was used to determine the CEA and CA 15-3 markers with the human kits using the radioimmunoassay method (RIA). Samples collected from extirpated tumors of mammary glands were histologically processed and classified as per WHO guidelines. The mean values of the carcinoembryonic antigen markers ± SD were as follows: control group 0.89 ± 0.79, group with mammary gland tumor 1.53 ± 1.15. The values of cancer antigen markers CA 15-3 ± SD were: 1.52 ± 0.66 and 2.87 ± 1.11, respectively. The statistical significance for the carcinoembryonic antigen marker between groups was P < 0.0001. The cancer antigen CA 15-3 values between groups were also statistically significant with P < 0.0001. The results of the present study show that there are significant differences in both antigens between the control group and groups with mammary gland tumor in dogs.","PeriodicalId":7379,"journal":{"name":"Acta Veterinaria","volume":"268 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138682796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ljubiša Veljović, Jelena Maksimović Zorić, Dimitrije Glišić, Jakov Nišavić, Jelena Maletić, Vesna Milićević
Schmallenberg disease is an infectious disease of cattle, sheep, and goats of viral aetiology caused by the Schmallenberg virus that belongs to the family Bunyaviridae and the genus Ortobunyavirus. Schmallenberg disease is a vector-borne disease transmitted by midges from the genus Culicoides but also by other hematophagous insects. The disease has a seasonal character and most often occurs in the warm months, from late spring to autumn. In this study, we investigated the seroprevalence of Schmallenberg disease in sheep in the epizootic area of Belgrade in the period from 2017 to 2022. A total of 600 sheep serums from the serum bank were tested, 100 serum samples from each year. Sera were tested by commercial ELISA test for the detection of specific antibodies against the Schmallenberg virus. The results showed an average annual seroprevalence of 24.5% in sheep in the Belgrade area in these six years with an increasing trend predicted for the next years.
{"title":"Seroprevalence of Schmallenberg Virus in Sheep in Belgrade Epizootic Area","authors":"Ljubiša Veljović, Jelena Maksimović Zorić, Dimitrije Glišić, Jakov Nišavić, Jelena Maletić, Vesna Milićević","doi":"10.2478/acve-2023-0038","DOIUrl":"https://doi.org/10.2478/acve-2023-0038","url":null,"abstract":"Schmallenberg disease is an infectious disease of cattle, sheep, and goats of viral aetiology caused by the Schmallenberg virus that belongs to the family <jats:italic>Bunyaviridae</jats:italic> and the genus <jats:italic>Ortobunyavirus</jats:italic>. Schmallenberg disease is a vector-borne disease transmitted by midges from the genus <jats:italic>Culicoides</jats:italic> but also by other hematophagous insects. The disease has a seasonal character and most often occurs in the warm months, from late spring to autumn. In this study, we investigated the seroprevalence of Schmallenberg disease in sheep in the epizootic area of Belgrade in the period from 2017 to 2022. A total of 600 sheep serums from the serum bank were tested, 100 serum samples from each year. Sera were tested by commercial ELISA test for the detection of specific antibodies against the Schmallenberg virus. The results showed an average annual seroprevalence of 24.5% in sheep in the Belgrade area in these six years with an increasing trend predicted for the next years.","PeriodicalId":7379,"journal":{"name":"Acta Veterinaria","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138682797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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