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Matrix Matters: novel insights for the extraction, preparation, and quantitation of microplastics in a freshwater mesocosm study 基质问题:在淡水生态研究中提取,制备和定量微塑料的新见解
Pub Date : 2023-06-05 DOI: 10.1186/s43591-023-00062-6
Jeanne M. Hankett, Jennifer L. Holtz, Imari Walker-Franklin, Kathryn Shaffer, Jerome Jourdan, Derek C. Batiste, Jessie M Garcia, Christine Kaczan, W. Wohlleben, L. Ferguson
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引用次数: 0
Quantification of Very Low Concentrations of Colloids with Light Scattering Applied to Micro(Nano)Plastics in Seawater 光散射法定量测定海水中微(纳米)塑料的极低浓度胶体
Pub Date : 2023-06-02 DOI: 10.3390/microplastics2020016
Gireeshkumar Balakrishnan, F. Lagarde, C. Chassenieux, T. Nicolai
The detection and quantification of micro(nano)plastics in the marine environment are essential requirements to understand the full impacts of plastic pollution on the ecosystem and human health. Here, static light scattering (SLS) and dynamic (DLS) light scattering techniques are assessed for their capacity to detect colloidal particles with diameters between d = 0.1 and 0.8 µm at very low concentrations in seawater. The detection limit of the apparatus was determined using model monodisperse spherical polystyrene latex particles with diameters of 0.2 µm and 0.5 µm. It is shown that the concentration and size of colloids can be determined down to about 10−6 g/L. Light scattering measurements on seawater obtained from different locations in Western Europe show that colloidal particles were detected with DLS in seawater filtered through 0.8 µm pore size filters. The concentration of these particles was not higher than 1 µg/L, with an average diameter of about 0.6 µm. We stress that these particles are not necessarily plastic. No particles were detected after filtration through 0.45 µm pore size filters.
海洋环境中微(纳米)塑料的检测和定量是了解塑料污染对生态系统和人类健康的全面影响的基本要求。本文评估了静态光散射(SLS)和动态光散射(DLS)技术在极低浓度海水中检测直径在d = 0.1至0.8µm之间的胶体颗粒的能力。采用直径分别为0.2µm和0.5µm的单分散球形聚苯乙烯乳胶模型确定仪器的检出限。结果表明,胶体的浓度和大小可以测定到10 ~ 6 g/L左右。对西欧不同地点海水的光散射测量表明,通过0.8µm孔径过滤器过滤后的海水中,DLS检测到胶体颗粒。这些颗粒的浓度不高于1µg/L,平均直径约为0.6µm。我们强调,这些颗粒不一定是塑料。孔径0.45µm的过滤器过滤后,未检测到任何颗粒。
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引用次数: 0
Microplastic paint particle production for spiking experiments; silicone rubber as application material provide high yield with low effort 微塑涂料颗粒生产,用于打钉实验;硅橡胶作为应用材料,具有低成本、高产量的特点
Pub Date : 2023-06-01 DOI: 10.1186/s43591-023-00061-7
Alexander S. Tagg
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引用次数: 1
Impact of Degradation of Polyethylene Particles on Their Cytotoxicity 聚乙烯颗粒降解对其细胞毒性的影响
Pub Date : 2023-05-22 DOI: 10.3390/microplastics2020015
Yudai Ikuno, H. Tsujino, Y. Haga, H. Asahara, K. Higashisaka, Y. Tsutsumi
Microplastics are ubiquitous in the environment, including in the ocean, soil, and air. Therefore, there are concerns regarding human exposure. Since it is known that the surface of microplastics in various environments is chemically deteriorated by external factors such as ultraviolet rays and waves, it is essential to evaluate the biological effects of degraded microplastics. In this study, we experimented by accelerating the degradation of polyethylene (PE) using vacuum ultraviolet light and prepared PE samples with different degrees of degradation. Then, we evaluated the effects of undegraded and variously degraded PE on cells using cytotoxicity tests. Based on the cytotoxicity test results, we saw a tendency for increased cytotoxicity with increasing degradation. Therefore, this study substantially links the deterioration of microplastics with their biological effects.
微塑料在环境中无处不在,包括在海洋、土壤和空气中。因此,有关于人类接触的担忧。众所周知,在各种环境中,微塑料的表面会受到紫外线和波浪等外部因素的化学变质,因此有必要评估降解微塑料的生物效应。在本研究中,我们利用真空紫外光加速降解聚乙烯(PE),制备了不同降解程度的PE样品。然后,我们使用细胞毒性试验评估了未降解和不同降解的PE对细胞的影响。根据细胞毒性测试结果,我们看到随着降解的增加,细胞毒性有增加的趋势。因此,这项研究实质上将微塑料的恶化与其生物效应联系起来。
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引用次数: 0
Microplastic Pollution in Sea Turtle Nests on the Beaches of Nautla and Vega de Alatorre, Veracruz 韦拉克鲁斯州纳特拉和维加德阿拉托雷海滩上的海龟巢中的微塑料污染
Pub Date : 2023-05-12 DOI: 10.3390/microplastics2020014
Belem Anahy Estrella-Jordan, F. Lango-Reynoso, M. Castañeda-Chávez, Jesús Montoya-Mendoza, David Reynier-Valdés
Microplastic contamination has become a topic of interest and concern worldwide due to its persistence and the possible effects it may cause to the environment. When microplastics are present, they can alter their physical properties, negatively affecting the surrounding fauna, such as sea turtles that use the beaches to nest in the sand. In this study, the exposure of sea turtle nests to microplastics on the beaches of Nautla and Vega de Alatorre, Veracruz, one of the main nesting areas for the green turtle Chelonia mydas, as well as Kemp’s ridley turtle Lepidochelis kempii from the Gulf of Mexico, was determined. Sand samples were obtained directly from the nests in situ on four beaches in the area and from two nesting pens, revealing the presence of microplastics in 100% of the nests in situ, with an average abundance of 2.43 ± 2.66 #MP/kg SS and a concentration of 0.00672 ± 0.02286 mgMP/kg SS, predominantly the form of foam, white in color, and from 1 to 2 mm in size.
微塑料污染由于其持久性和可能对环境造成的影响,已成为全世界关注的话题。当微塑料存在时,它们会改变其物理特性,对周围的动物群产生负面影响,比如海龟利用海滩在沙滩上筑巢。在这项研究中,确定了韦拉克鲁斯州诺特拉和维加德阿拉托雷海滩上的海龟巢暴露于微塑料的情况。韦拉克鲁斯州是绿龟龟mydas的主要筑巢区之一,以及来自墨西哥湾的肯普雷氏龟Lepidochelis kempii。直接从该地区4个海滩的原位巢和2个巢圈中获得的沙样显示,100%的原位巢中存在微塑料,平均丰度为2.43±2.66 #MP/kg SS,浓度为0.00672±0.02286 mgMP/kg SS,主要以泡沫形式存在,颜色为白色,大小为1 ~ 2 mm。
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引用次数: 1
Protocol for the production of micro- and nanoplastic test materials 微塑料和纳米塑料试验材料生产规程
Pub Date : 2023-05-05 DOI: 10.1186/s43591-023-00058-2
Luke A. Parker, E. M. Höppener, Edward van Amelrooij, S. Henke, I. Kooter, K. Grigoriadi, Merel G. A. Nooijens, A. M. Brunner, A. Boersma
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引用次数: 1
Development of a machine learning-based method for the analysis of microplastics in environmental samples using µ-Raman spectroscopy 开发了一种基于机器学习的方法,用于使用微拉曼光谱分析环境样品中的微塑料
Pub Date : 2023-04-25 DOI: 10.1186/s43591-023-00057-3
Felix Weber, Andreas Zinnen, Jutta Kerpen
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引用次数: 0
Hot or not: systematic review and laboratory evaluation of the hot needle test for microplastic identification 热或不热:微塑料鉴定热针试验的系统综述和实验室评价
Pub Date : 2023-04-24 DOI: 10.1186/s43591-023-00056-4
B. Beckingham, Adriana Apintiloaiei, C. Moore, Jay Brandes
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引用次数: 5
Exploring expert perceptions about microplastics: from sources to potential solutions 探索专家对微塑料的看法:从来源到潜在解决方案
Pub Date : 2023-04-11 DOI: 10.1186/s43591-023-00055-5
Maja Grünzner, S. Pahl, M. White, Richard C. Thompson
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引用次数: 0
Microplastic Contamination in Cultured Mussels and Pearl Oysters in Greece 希腊养殖贻贝和珍珠贝中的微塑料污染
Pub Date : 2023-04-03 DOI: 10.3390/microplastics2020013
Stefania M. Manolaki, Dimitra Chatzivasileiou, Maria Lampa, Panagiotis D. Dimitriou, A. Philippidis, Ioannis Karakassis, N. Papageorgiou
Τhis study aims to measure the abundance of microplastic (MP) particles in the soft tissue of mussel (Mytilus galloprovincialis) and pearl oyster (Pinctada imbricata radiata) specimens. Samples were collected at four sites in Greece (Sagiada, Malesina, Elounda, Rhodes) from wild and farmed populations. The identification of MPs was accomplished by Raman spectroscopy. Comparisons were made between the two different species where the two species co-existed (Malesina), between the four study sites (five sampling stations) in relation to P. imbricata radiata individuals, and also in every station for the different MP types found. For the specimens from Malesina, M. galloprovincialis had more MPs in their soft tissue compared to P. imbricata radiata. Microfibers were found in abundance in M. galloprovincialis, while microfragments were found in P. imbricata radiata specimens. The main MP type found in P. imbricata radiata specimens was microfragments in all five sampling stations, and ranged between 1.54 ± 0.63 (Rhodes-baskets) and 3.56 ± 0.35 (Sagiada) MP particles/g. While the samples of mussels and pearl oysters were similar in age, the differences found in the concentrations of MPs appears to be due to their different farming methods and location characteristics concerning the five sampling stations of pearl oysters. This study indicates that the culturing system does not affect MP concentration in bivalves, and further investigation is needed to find the most appropriate method to limit and reduce MPs that end up in the farmed organisms.
Τhis研究旨在测量贻贝(Mytilus galloprovincialis)和珍珠牡蛎(Pinctada imbricata radiata)标本软组织中微塑料(MP)颗粒的丰度。在希腊的四个地点(Sagiada、Malesina、Elounda和Rhodes)从野生和养殖种群中采集样本。用拉曼光谱法对MPs进行了鉴定。比较了两种共存的两种(马勒斯纳)、4个研究点(5个采样点)与辐射斑杨个体的关系,以及每个采样点所发现的不同MP类型。在马列西那岛的标本中,加洛省m.g loloprovincialis在其软组织中的MPs含量高于辐射P. imbricata radiata。微纤维在加洛省密螺旋藻中大量存在,而微碎片在辐射密螺旋藻中大量存在。5个采样点辐射柽柳标本的主要MP类型均为微片段,分布在1.54±0.63 (rhodes -筐)和3.56±0.35 (Sagiada)之间。贻贝和珍珠牡蛎的样本年龄相近,但MPs浓度的差异似乎是由于五个珍珠牡蛎采样站不同的养殖方法和位置特征所致。该研究表明,培养系统不影响双壳类动物的MPs浓度,需要进一步研究以找到最合适的方法来限制和减少最终在养殖生物中的MPs。
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引用次数: 2
期刊
Microplastics and nanoplastics
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