Pub Date : 2021-01-01DOI: 10.1007/978-1-0716-1637-6_21
D. Pamies, M. Chesnut, Hélène Paschoud, M. Zurich, T. Hartung, H. Hogberg
{"title":"Quantification of Oligodendrocytes and Myelin in Human iPSC-Derived 3D Brain Cell Cultures (BrainSpheres)","authors":"D. Pamies, M. Chesnut, Hélène Paschoud, M. Zurich, T. Hartung, H. Hogberg","doi":"10.1007/978-1-0716-1637-6_21","DOIUrl":"https://doi.org/10.1007/978-1-0716-1637-6_21","url":null,"abstract":"","PeriodicalId":74285,"journal":{"name":"Neuromethods","volume":"47 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"51710504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Omamuyovwi M Ijomone, Priscila Gubert, Comfort O A Okoh, Alexandre M Varão, Leandro de O Amara, Oritoke M Aluko, Michael Aschner
The nematode Caenorhabditis elegans (C. elegans) is a prevailing model which is commonly utilized in a variety of biomedical research arenas, including neuroscience. Due to its transparency and simplicity, it is becoming a choice model organism for conducting imaging and behavioral assessment crucial to understanding the intricacies of the nervous system. Here, the methods required for neuronal characterization using fluorescent proteins and behavioral tasks are described. These are simplified protocols using fluorescent microscopy and behavioral assays to examine neuronal connections and associated neurotransmitter systems involved in normal physiology and aberrant pathology of the nervous system. Our aim is to make available to readers some streamlined and replicable procedures using C. elegans models as well as highlighting some of the limitations.
{"title":"Application of Fluorescence Microscopy and Behavioral Assays to Demonstrating Neuronal Connectomes and Neurotransmitter Systems in <i>C. elegans</i>.","authors":"Omamuyovwi M Ijomone, Priscila Gubert, Comfort O A Okoh, Alexandre M Varão, Leandro de O Amara, Oritoke M Aluko, Michael Aschner","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The nematode <i>Caenorhabditis elegans</i> (<i>C. elegans</i>) is a prevailing model which is commonly utilized in a variety of biomedical research arenas, including neuroscience. Due to its transparency and simplicity, it is becoming a choice model organism for conducting imaging and behavioral assessment crucial to understanding the intricacies of the nervous system. Here, the methods required for neuronal characterization using fluorescent proteins and behavioral tasks are described. These are simplified protocols using fluorescent microscopy and behavioral assays to examine neuronal connections and associated neurotransmitter systems involved in normal physiology and aberrant pathology of the nervous system. Our aim is to make available to readers some streamlined and replicable procedures using <i>C. elegans</i> models as well as highlighting some of the limitations.</p>","PeriodicalId":74285,"journal":{"name":"Neuromethods","volume":"172 ","pages":"399-426"},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8575032/pdf/nihms-1737525.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39604355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.1007/978-1-0716-0428-1_15
R. Pelc, W. Walz, J. R. Doucette
{"title":"Correction to: Neurohistology and Imaging Techniques","authors":"R. Pelc, W. Walz, J. R. Doucette","doi":"10.1007/978-1-0716-0428-1_15","DOIUrl":"https://doi.org/10.1007/978-1-0716-0428-1_15","url":null,"abstract":"","PeriodicalId":74285,"journal":{"name":"Neuromethods","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"51706521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.1007/978-1-0716-1319-1_2
P. Lewczuk
{"title":"Nephelometry and Turbidimetry: Methods to Quantify Albumin and Immunoglobulins Concentrations in Clinical Neurochemistry","authors":"P. Lewczuk","doi":"10.1007/978-1-0716-1319-1_2","DOIUrl":"https://doi.org/10.1007/978-1-0716-1319-1_2","url":null,"abstract":"","PeriodicalId":74285,"journal":{"name":"Neuromethods","volume":"34 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"51707103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.1007/978-1-0716-1530-0_14
M. El‐Ghanem, Muhammad H Niazi, K. Cockroft
{"title":"Carotid Cavernous Fistulas","authors":"M. El‐Ghanem, Muhammad H Niazi, K. Cockroft","doi":"10.1007/978-1-0716-1530-0_14","DOIUrl":"https://doi.org/10.1007/978-1-0716-1530-0_14","url":null,"abstract":"","PeriodicalId":74285,"journal":{"name":"Neuromethods","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"51708887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.1007/978-1-0716-1589-8_3
Andrea K H Stavoe, E. Holzbaur
{"title":"Live Imaging of Autophagosome Biogenesis and Maturation in Primary Neurons","authors":"Andrea K H Stavoe, E. Holzbaur","doi":"10.1007/978-1-0716-1589-8_3","DOIUrl":"https://doi.org/10.1007/978-1-0716-1589-8_3","url":null,"abstract":"","PeriodicalId":74285,"journal":{"name":"Neuromethods","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"51709262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.1007/978-1-0716-1637-6_7
Susanna B. Park, C. Renn, J. Lees, S. Dorsey, G. Cavaletti, V. Carozzi
{"title":"Electrophysiological Assessments in and in Rodent Models of Chemotherapy-Induced Painful Peripheral","authors":"Susanna B. Park, C. Renn, J. Lees, S. Dorsey, G. Cavaletti, V. Carozzi","doi":"10.1007/978-1-0716-1637-6_7","DOIUrl":"https://doi.org/10.1007/978-1-0716-1637-6_7","url":null,"abstract":"","PeriodicalId":74285,"journal":{"name":"Neuromethods","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"51710625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.1007/978-1-0716-1637-6_5
T. Venet, Aurélie Thomas, L. Wathier, B. Pouyatos
{"title":"DPOAEs for the Assessment of Noise- or Toxicant-Induced Cochlear Damage in the","authors":"T. Venet, Aurélie Thomas, L. Wathier, B. Pouyatos","doi":"10.1007/978-1-0716-1637-6_5","DOIUrl":"https://doi.org/10.1007/978-1-0716-1637-6_5","url":null,"abstract":"","PeriodicalId":74285,"journal":{"name":"Neuromethods","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"51711004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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