Omamuyovwi M Ijomone, Priscila Gubert, Comfort O A Okoh, Alexandre M Varão, Leandro de O Amara, Oritoke M Aluko, Michael Aschner
The nematode Caenorhabditis elegans (C. elegans) is a prevailing model which is commonly utilized in a variety of biomedical research arenas, including neuroscience. Due to its transparency and simplicity, it is becoming a choice model organism for conducting imaging and behavioral assessment crucial to understanding the intricacies of the nervous system. Here, the methods required for neuronal characterization using fluorescent proteins and behavioral tasks are described. These are simplified protocols using fluorescent microscopy and behavioral assays to examine neuronal connections and associated neurotransmitter systems involved in normal physiology and aberrant pathology of the nervous system. Our aim is to make available to readers some streamlined and replicable procedures using C. elegans models as well as highlighting some of the limitations.
{"title":"Application of Fluorescence Microscopy and Behavioral Assays to Demonstrating Neuronal Connectomes and Neurotransmitter Systems in <i>C. elegans</i>.","authors":"Omamuyovwi M Ijomone, Priscila Gubert, Comfort O A Okoh, Alexandre M Varão, Leandro de O Amara, Oritoke M Aluko, Michael Aschner","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The nematode <i>Caenorhabditis elegans</i> (<i>C. elegans</i>) is a prevailing model which is commonly utilized in a variety of biomedical research arenas, including neuroscience. Due to its transparency and simplicity, it is becoming a choice model organism for conducting imaging and behavioral assessment crucial to understanding the intricacies of the nervous system. Here, the methods required for neuronal characterization using fluorescent proteins and behavioral tasks are described. These are simplified protocols using fluorescent microscopy and behavioral assays to examine neuronal connections and associated neurotransmitter systems involved in normal physiology and aberrant pathology of the nervous system. Our aim is to make available to readers some streamlined and replicable procedures using <i>C. elegans</i> models as well as highlighting some of the limitations.</p>","PeriodicalId":74285,"journal":{"name":"Neuromethods","volume":"172 ","pages":"399-426"},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8575032/pdf/nihms-1737525.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39604355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.1007/978-1-0716-1522-5_8
V. Fernández-Dueñas, T. Durroux, F. Ciruela
{"title":"Time-Resolved Fluorescence Resonance Energy Transfer Using Fluorescent Ligands to Study Native G Protein-Coupled Receptor Heteromerization in Brain","authors":"V. Fernández-Dueñas, T. Durroux, F. Ciruela","doi":"10.1007/978-1-0716-1522-5_8","DOIUrl":"https://doi.org/10.1007/978-1-0716-1522-5_8","url":null,"abstract":"","PeriodicalId":74285,"journal":{"name":"Neuromethods","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"51708836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.1007/978-1-0716-1530-0_15
B. M. Grory, Nasir Fakhri, S. Yaghi
{"title":"Thrombotic Strokes","authors":"B. M. Grory, Nasir Fakhri, S. Yaghi","doi":"10.1007/978-1-0716-1530-0_15","DOIUrl":"https://doi.org/10.1007/978-1-0716-1530-0_15","url":null,"abstract":"","PeriodicalId":74285,"journal":{"name":"Neuromethods","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"51708898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.1007/978-1-0716-1530-0_21
A. Gusdon, Mackenzie Lerario, E. Lavi, G. Bernardini
{"title":"Central Nervous System Vasculitis: Primary Angiitis of the Central Nervous System","authors":"A. Gusdon, Mackenzie Lerario, E. Lavi, G. Bernardini","doi":"10.1007/978-1-0716-1530-0_21","DOIUrl":"https://doi.org/10.1007/978-1-0716-1530-0_21","url":null,"abstract":"","PeriodicalId":74285,"journal":{"name":"Neuromethods","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"51708989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.1007/978-1-0716-1589-8_7
A. Lopez, A. Fleming, D. Rubinsztein
{"title":"Measurement of Neuronal Clearance In Vivo","authors":"A. Lopez, A. Fleming, D. Rubinsztein","doi":"10.1007/978-1-0716-1589-8_7","DOIUrl":"https://doi.org/10.1007/978-1-0716-1589-8_7","url":null,"abstract":"","PeriodicalId":74285,"journal":{"name":"Neuromethods","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"51709399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.1007/978-1-0716-1522-5_22
Megumu Takahashi, Yoko Ishida, N. Kataoka, Kazuhiro Nakamura, H. Hioki
{"title":"Efficient Labeling of Neurons and Identification of Postsynaptic Sites Using Adeno-Associated Virus Vector","authors":"Megumu Takahashi, Yoko Ishida, N. Kataoka, Kazuhiro Nakamura, H. Hioki","doi":"10.1007/978-1-0716-1522-5_22","DOIUrl":"https://doi.org/10.1007/978-1-0716-1522-5_22","url":null,"abstract":"","PeriodicalId":74285,"journal":{"name":"Neuromethods","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"51708804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.1007/978-1-0716-1522-5_30
M. López-Cano, V. Fernández-Dueñas, F. Ciruela
{"title":"Optical Control of Brain Receptors Using Photoactive Drugs in Behaving Animals","authors":"M. López-Cano, V. Fernández-Dueñas, F. Ciruela","doi":"10.1007/978-1-0716-1522-5_30","DOIUrl":"https://doi.org/10.1007/978-1-0716-1522-5_30","url":null,"abstract":"","PeriodicalId":74285,"journal":{"name":"Neuromethods","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"51708818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.1007/978-1-0716-1522-5_32
Jaume J. Taura, V. Fernández-Dueñas, F. Ciruela
{"title":"Monitoring GPCR-Mediated cAMP Accumulation in Rat Striatal Synaptosomes","authors":"Jaume J. Taura, V. Fernández-Dueñas, F. Ciruela","doi":"10.1007/978-1-0716-1522-5_32","DOIUrl":"https://doi.org/10.1007/978-1-0716-1522-5_32","url":null,"abstract":"","PeriodicalId":74285,"journal":{"name":"Neuromethods","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"51708829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.1007/978-1-0716-1530-0_17
M. Padrick, S. Lahiri, K. Schlick
{"title":"Embolic Ischemic Stroke","authors":"M. Padrick, S. Lahiri, K. Schlick","doi":"10.1007/978-1-0716-1530-0_17","DOIUrl":"https://doi.org/10.1007/978-1-0716-1530-0_17","url":null,"abstract":"","PeriodicalId":74285,"journal":{"name":"Neuromethods","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"51708915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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